CN105664020A

CN105664020A - Preparation method of medicine for treating peritoneal mesothelioma

Info

Publication number: CN105664020A
Application number: CN201610070137.3A
Authority: CN
Inventors: 孙超
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-01-29
Filing date: 2016-01-29
Publication date: 2016-06-15

Abstract

The invention provides a preparation method of a medicine for treating peritoneal mesothelioma and belongs to the field of traditional Chinese medicines. The medicine is prepared from traditional Chinese medicine raw materials in parts by weight as follows: 10-20 parts of roots of kirilow rhodiola, 15-25 parts of ganoderma lucidum, 5-10 parts of pepper, 5-10 parts of prepared rehmannia roots, 20-30 parts of membranous milkvetch roots, 15-25 parts of poria cocos, 10-20 parts of honey-fried liquorice roots, 10-20 parts of indian iphigenia bulb, 15-25 parts of snakegourd fruits, 10-20 parts of glossy privet fruits, 15-20 parts of zedoary, 20-30 parts of suberect spatholobus stems, 20-30 parts of processed pangolin scales, 20-25 parts of twotooth achyranthes roots, 30-40 parts of white peony roots, 20-30 parts of danshen roots, 15-20 parts of Japanese thistle herb or roots, 20-30 parts of thinleaf milkwort root-bark, 20-25 parts of radix cocculi trilobi and 10-15 parts of dahurian patrinia herbs. The medicine raw materials are dialectically matched and perform the efficacy of dredging meridians, promoting the secretion of body fluids, resisting inflammation, removing toxins, activating blood, relieving pain, improving the immunity, inhibiting growth of tumor cancers and the like, and the medicine has a remarkable curative effect on peritoneal mesothelioma.

Description

It is used for the treatment of the preparation method of the medicine of peritoneal mesothelium knurl

Technical field

The present invention relates to technical field of traditional Chinese medicines, it is specifically related to the preparation method of a kind of medicine being used for the treatment of peritoneal mesothelium knurl.

Background technology

Peritoneal mesothelium knurl, also known as Primary peritoneal mesothelioma, is a kind of tumour stemming from pleura or peritoneal surface mesothelial cell, refers to the tumour being primary in the mesothelial cell with two-way differentiation potential or a subcutaneous Primitive mcsenchymal cell. Its main clinical manifestation is stomachache, abdominal distension, feel sick, diarrhoea or constipation, urinary tract irritation, generates heat, becomes thin, anaemia, indivedual patient clinical manifestation such as have hypoglycemia, diffusivity belly ossified.

At present, peritoneal mesothelium knurl still lacks standardization methods for the treatment of, mainly through the comprehensive therapy of radiation and chemotherapy after excision, art, to peritoneal mesothelium knurl that is optimum and biological behaviour low potential malignancy, excision curative effect is very good, can excision again if any recurrence, need the X line being equipped with 60Co or 186kV as irradiation chemotherapy for pernicious peritoneal mesothelium knurl, its 3 years survival rates can reach more than 60%, within 5 years, survival rate is lower, illness can not be cured, and poor for patient's operative results that physique is poor.

Summary of the invention

The present invention proposes the preparation method of a kind of medicine being used for the treatment of peritoneal mesothelium knurl, the medicine that this preparation method obtains can lead to that network promotes the production of body fluid, the growth of anti inflammatory detoxication, lifting immunizing power, anticancer.

The technical solution used in the present invention is as follows:

The preparation method being used for the treatment of the medicine of peritoneal mesothelium knurl, comprises the following steps successively:

The first step, choose Chinese medicine material

Take Root of Kirilow Rhodiola 10～20 parts respectively, glossy ganoderma 15～25 parts, 5～10 parts, pepper, 5～10 parts, cultivated land, the Radix Astragali 20～30 parts, 15～25 parts, Poria cocos, Radix Glycyrrhizae Preparata 10～20 parts, Pseudobulbus Cremastrae Seu Pleiones 10～20 parts, whole Fructus Trichosanthis 15～25 parts, Glossy Privet Fruit 10～20 parts, curcuma zedoary 15～20 parts, Yunnan Caulis Spatholobi 20～30 parts, big gun mountain first 20～30 parts, Radix Achyranthis Bidentatae 20～25 parts, the root of herbaceous peony 30～40 parts, the red sage root 20～30 parts, setose thistle 15～20 parts, polygala root 20～30 parts, Radix Cocculi Orbiculati 20～25 parts and Herba Patriniae 10～15 parts,

2nd step, enzymolysis processing

Measured for the above-mentioned title Radix Astragali, glossy ganoderma, the red sage root, Poria cocos, Radix Glycyrrhizae Preparata, curcuma zedoary, the root of herbaceous peony, big gun mountain first, cultivated land are mixed and pulverizes, obtain mixture one, polygalacturonase, cellulase and water is added in mixture one, the add-on of described polygalacturonase and cellulase is the 2% of mixture one weight, the add-on of described water is 7～8 times of mixture one weight, controls water temperature 40-50 DEG C, pH4-6, enzymolysis 3-5 hour, obtains enzymolysis solution;

3rd step, alcohol extracting method process

Root of Kirilow Rhodiola, pepper, Pseudobulbus Cremastrae Seu Pleiones, whole Fructus Trichosanthis, Glossy Privet Fruit, Yunnan Caulis Spatholobi, Radix Achyranthis Bidentatae, setose thistle, polygala root, Radix Cocculi Orbiculati, Herba Patriniae are mixed and pulverizes, obtain mixture two, the extraction using alcohol of massfraction 40-60% is added in mixture two, the add-on of ethanol is 7～9 times of mixture two weight, extracts twice, and each extraction time is 1-2 hour, after oven dry, add water refrigeration 5-6 hour, is filtered to remove impurity, obtains alcohol extract;

4th step, microwave drying

After enzymolysis solution and alcohol extract mixing simmer down to pasty state, add microwave dryer, microwave frequency 2.45GHz, dry 20 minutes, obtain mixture three;

5th step, gained mixture three is mixed with honey, make pill, obtain medicine.

Preferably, the weight fraction of Chinese medicine material is: Root of Kirilow Rhodiola 15 parts, glossy ganoderma 20 parts, 8 parts, pepper, 8 parts, cultivated land, the Radix Astragali 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, curcuma zedoary 18 parts, Yunnan Caulis Spatholobi 25 parts, big gun mountain first 25 parts, Radix Achyranthis Bidentatae 22 parts, the root of herbaceous peony 35 parts, the red sage root 25 parts, setose thistle 18 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts

The pharmacology of the present invention and Advantageous Effects:

Root of Kirilow Rhodiola have strengthen the body resistance to consolidate the constitution, effect that logical god is not old;

Glossy ganoderma has effect of benefiting vital QI and blood;

Pepper warming middle-JIAO to relieve pain, the effect driving away long-pending gas;

Cultivated land has essence moist, beneficial of enriching blood to fill out effect of marrow;

The Radix Astragali has and drives wind fortune poison, mends Yuanyang, fills effect of space between skin and muscles;

Poria cocos has tranquillizing by calming the heart, relieve internal heat effect anticancer, strengthening immunity;

Radix Glycyrrhizae Preparata has effect of anti inflammatory detoxication, anticancer growth;

Pseudobulbus Cremastrae Seu Pleiones has relievings asthma and pain relieving, antitumor effect;

Whole Fructus Trichosanthis has clearing heat and moistening dryness, promotes the production of body fluid to quench thirst, effect of apocenosis detumescence;

Glossy Privet Fruit has effect of enriching yin and nourishing kidney, nourishing the liver tomorrow;

Curcuma zedoary has antitoxin anti-inflammatory, promoting the circulation of QI to relieve pain, antitumor effect;

Yunnan Caulis Spatholobi is enriched blood and invigorates blood circulation, effect of dredging the meridian;

Big gun mountain first has effect that detumescence is suppurated, scattered silt leads to network, invigorating blood circulation analgesia;

Radix Achyranthis Bidentatae has effect of promoting blood circulation to remove blood stasis, clearing damp diuresis, heat-clearing solution;

The root of herbaceous peony has effect of flat liver pain relieving, nourishing blood for regulating menstruation, astringing YIN to stop sweating;

The red sage root has scattered silt of invigorating blood circulation, detumescence hemostasis, anti-inflammatory analgetic, antibacterial effect;

Setose thistle has antibacterial anti hemorrhagic, supports effect that essence breaks the stasis of blood;

Effect that polygala root has intelligence development of calming the nerves, eliminates the phlegm, subsides a swelling;

The favourable water of Radix Cocculi Orbiculati stimulates the menstrual flow, effect of alleviating pain and detumescence;

Herba Patriniae has effect of blood clearing heat and detoxicating, cool, the carbuncle that disappears apocenosis, stasis-dispelling and pain-killing.

Above-mentioned medicinal material, through dialectical compatibility, plays the effects such as logical network promotes the production of body fluid, anti inflammatory detoxication, promoting blood circulation and stopping pain, lifting immunizing power, anticancer growth altogether, and peritoneal mesothelium knurl is evident in efficacy.

Due to Chinese medicine compound prescription complicated component, its effective constituent of some medicine is all wrapped in cell walls, and cell walls is by Mierocrystalline cellulose, the materials such as pectin substance form dense structure, in the process extracting medicinal material effective constituent, effective constituent is when spreading to Extraction medium, the resistance of cell walls must be overcome, select the cellulase of hydrocellulose, the polygalacturonase etc. of hydrolysis of pectin matter, cell walls can be decomposed by enzyme reaction, more effective extracting effective components, also the molten property of the alcohol that in the medicine having, effective constituent has had, ethanol extract from water precipitation can be utilized to obtain effective constituent, effective constituent in Chinese medicine can not all be extracted by traditional decocting method, reduce the curative effect of Chinese medicine, if making pill after medicinal material is simply pulverized, effective constituent can be effectively used, but the vegetable fibre contained in medicinal material can increase the weight of the stomach burden of patient, the present invention is with enzymolysis process and ethanol extract from water precipitation combined extracting traditional Chinese medicine effective ingredient.

Part medicine is carried out enzymolysis process extracting effective components by one aspect of the present invention, on the other hand to the heavy method extracting effective components of part medicine alcoholysis water, and extraction efficiency height, and the property of medicine can not be destroyed.

Clinical data

1, case selection: the medical person of the random outpatient service selecting 50 examples to suffer from peritoneal mesothelium knurl, man 28 example, female 22 example, minimum 5 years old of age, the oldest 48 years old;

2, clinical primary symptom: poor appetite, abdominal distension, abdominal mass, One's spirits are drooping, weak;

3, methods for the treatment of: boiling water is taken after mixing it with water pill, one time 1, twice on the one;

4, curative effect judging standard: (taking take 28 days as one the course for the treatment of standard)

Cure: symptom all disappears, without recurrence in 5 years;

Effective: symptom disappears substantially, the state of an illness obviously takes a turn for the better;

Invalid: symptom is rear unchanged before the treatment.

5, result for the treatment of

As shown in table 1, adopt medicine pill of the present invention to treat five courses for the treatment of, cure 25 examples, effective 20 examples, invalid 5 examples, always have efficiency 90%, it does not have the patient of inadaptation occur.

Table 1

Patient	Number of cases	Cure/example	Effectively/example	Invalid/example	Total effectively example
						The male sex	28	18	8	2	26
Women	22	9	10	3	19
						Amount to	50	27	18	5	45

8, model case

Model case one:

Opening certain, 20 years old, in June, 2010 goes to a doctor;

Main suit: stomachache, abdominal distension, face pale;

Checking: full abdomen tenderness, peritoneocentesis ascites is orange, finds a large amount of malignant mesothe cell;

Treatment: oral, one time 1, twice on the one. After patient takes 5 courses for the treatment of continuously, tumor disappearance, observes and has no recurrence in 5 years.

Model case two:

Lee, 43 years old, in March, 2009 went to a doctor;

Main suit: feel sick, poor appetite, abdominal distension, unable, idol have fever;

Checking: intraperitoneal is long-pending liquid obviously, nethike embrane and mesentery root are shown in a small amount of about 0.1cm size tumour, tendency malignant Peritoneal Mesothelioma;

Treatment: oral, one time 1, twice on the one. After patient takes 5 courses for the treatment of continuously, tumor disappearance, complexion is gradually good, and seroperitoneum also disappears substantially;

Efficacy determination: effectively.

Model case three:

Mulberry, 5 years old, in March, 2012 went to a doctor;

Main suit: diarrhoea, drowsiness, poor appetite;

Check: abdominal cavity has long-pending liquid, ascites to be orange, finds a large amount of mesothelioma cell;

Treatment: oral, one time 1, twice on the one. After patient takes 5 courses for the treatment of continuously, tumor disappearance, seroperitoneum also disappears substantially, observes 5 years without recurrence.

Embodiment

Embodiment 1

The first step, take the Radix Astragali 25 parts, glossy ganoderma 20 parts, the red sage root 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 25 parts, the mixing of 7 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 40-50 DEG C, pH4-6, enzymolysis 3-5 hour, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 15 parts, 7 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add amount of alcohol be medicinal material amount 7-9 doubly, extraction time is 1-2 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

3rd step, by after concentrated to enzymolysis solution and alcohol extract mixing, microwave drying, microwave frequency 2.45GHz, dry 20 minutes, obtains mixture three;

4th step, adding a certain amount of honey in mixture three, obtain medicine pill 56, have breath wind antispastic, dispersing pathogen accumulation, the effect removed obstruction in channels to relieve pain, have more antineoplastic effect.

Embodiment 2

The first step, take the Radix Astragali 20 parts, glossy ganoderma 25 parts, the red sage root 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 25 parts, the mixing of 10 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 40 DEG C, pH6, enzymolysis 5 hours, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 10 parts, 5 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add amount of alcohol be medicinal material amount 7-9 doubly, extraction time is 1-2 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Embodiment 3

The first step, take the Radix Astragali 25 parts, glossy ganoderma 15 parts, the red sage root 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 25 parts, the mixing of 7 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 50 DEG C, pH4, enzymolysis 3 hours, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 15 parts, 7 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 10 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add amount of alcohol be medicinal material amount 7-9 doubly, extraction time is 1-2 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Embodiment 4

The first step, take the Radix Astragali 25 parts, glossy ganoderma 20 parts, the red sage root 20 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 20 parts, the mixing of 5 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add the water of pharmaceutical quantities 7 times, control water temperature 50 DEG C, pH5, enzymolysis 4 hours, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 15 parts, 7 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 15 parts, polygala root 20 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add amount of alcohol be medicinal material amount 7-9 doubly, extraction time is 1-2 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Embodiment 5

The first step, take the Radix Astragali 25 parts, glossy ganoderma 20 parts, the red sage root 25 parts, 25 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 20 parts, the mixing of 7 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 50 DEG C, pH6, enzymolysis 5 hours, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 15 parts, 10 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 20 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add that amount of alcohol is medicinal material amount 8 times, extraction time is 2 hours, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Embodiment 6

The first step, take the Radix Astragali 20 parts, glossy ganoderma 20 parts, the red sage root 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 35 parts, big gun mountain first 25 parts, the mixing of 10 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 40-50 DEG C, pH4-6, enzymolysis 3-5 hour, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 15 parts, 7 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 25 parts and Herba Patriniae 15 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add that amount of alcohol is medicinal material amount 7 times, extraction time is 1 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Embodiment 7

The first step, take the Radix Astragali 20 parts, glossy ganoderma 20 parts, the red sage root 20 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, curcuma zedoary 17 parts, the root of herbaceous peony 30 parts, big gun mountain first 20 parts, the mixing of 7 parts, cultivated land, the polygalacturonase of medicinal material amount 2%, the cellulase of 2% is added after pulverizing, add pharmaceutical quantities 7-8 water doubly, control water temperature 40-50 DEG C, pH4-6, enzymolysis 3-5 hour, obtains enzymolysis solution;

2nd step, take Root of Kirilow Rhodiola 10 parts, 7 parts, pepper, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, Yunnan Caulis Spatholobi 25 parts, Radix Achyranthis Bidentatae 22 parts, setose thistle 17 parts, polygala root 25 parts, Radix Cocculi Orbiculati 20 parts and Herba Patriniae 12 parts, pulverize after mixing, add the extraction using alcohol 2 times of 40-60%, add amount of alcohol be medicinal material amount 7-9 doubly, extraction time is 1-2 hour, and after concentrated, add water refrigeration 24-36 hour, it is filtered to remove impurity, obtains alcohol extract;

Claims

1. one kind is used for the treatment of the preparation method of the medicine of peritoneal mesothelium knurl, it is characterised in that, comprise the following steps successively:

The first step, choose Chinese medicine material

2nd step, enzymolysis processing

3rd step, alcohol extracting method process

4th step, microwave drying

5th step, gained mixture three is mixed with honey, make pill, obtain medicine.

2. the preparation method of the medicine being used for the treatment of peritoneal mesothelium knurl according to claim 1, it is characterised in that: the weight fraction of Chinese medicine material is: Root of Kirilow Rhodiola 15 parts, glossy ganoderma 20 parts, 8 parts, pepper, 8 parts, cultivated land, the Radix Astragali 25 parts, 20 parts, Poria cocos, Radix Glycyrrhizae Preparata 15 parts, Pseudobulbus Cremastrae Seu Pleiones 15 parts, whole Fructus Trichosanthis 20 parts, Glossy Privet Fruit 15 parts, curcuma zedoary 18 parts, Yunnan Caulis Spatholobi 25 parts, big gun mountain first 25 parts, Radix Achyranthis Bidentatae 22 parts, the root of herbaceous peony 35 parts, the red sage root 25 parts, setose thistle 18 parts, polygala root 25 parts, Radix Cocculi Orbiculati 22 parts and Herba Patriniae 12 parts.