CN105661084A - Feed capable of relieving symptom of manganism of chickens, as well as preparation method and using method of feed - Google Patents
Feed capable of relieving symptom of manganism of chickens, as well as preparation method and using method of feed Download PDFInfo
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- CN105661084A CN105661084A CN201610004137.3A CN201610004137A CN105661084A CN 105661084 A CN105661084 A CN 105661084A CN 201610004137 A CN201610004137 A CN 201610004137A CN 105661084 A CN105661084 A CN 105661084A
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 43
- 208000024891 symptom Diseases 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 13
- 235000013330 chicken meat Nutrition 0.000 title abstract 10
- 208000005903 Manganese Poisoning Diseases 0.000 title abstract 6
- 238000000855 fermentation Methods 0.000 claims abstract description 45
- 230000004151 fermentation Effects 0.000 claims abstract description 42
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000011572 manganese Substances 0.000 claims abstract description 34
- 229910052748 manganese Inorganic materials 0.000 claims abstract description 34
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 29
- 244000062793 Sorghum vulgare Species 0.000 claims abstract description 11
- 240000008042 Zea mays Species 0.000 claims abstract description 11
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 11
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 11
- 235000005822 corn Nutrition 0.000 claims abstract description 11
- 235000019713 millet Nutrition 0.000 claims abstract description 11
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims abstract description 7
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims abstract description 7
- 235000002867 manganese chloride Nutrition 0.000 claims abstract description 7
- 239000011565 manganese chloride Substances 0.000 claims abstract description 7
- 229940099607 manganese chloride Drugs 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 57
- 241000195493 Cryptophyta Species 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 21
- 230000001988 toxicity Effects 0.000 claims description 21
- 231100000419 toxicity Toxicity 0.000 claims description 21
- 230000004913 activation Effects 0.000 claims description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 13
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 244000046109 Sorghum vulgare var. nervosum Species 0.000 claims description 10
- 238000002525 ultrasonication Methods 0.000 claims description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 abstract description 7
- 244000144977 poultry Species 0.000 abstract description 6
- 239000012530 fluid Substances 0.000 abstract 4
- 240000001046 Lactobacillus acidophilus Species 0.000 abstract 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 abstract 1
- 244000046052 Phaseolus vulgaris Species 0.000 abstract 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 abstract 1
- 240000006394 Sorghum bicolor Species 0.000 abstract 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 abstract 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 6
- 231100000572 poisoning Toxicity 0.000 description 6
- 230000000607 poisoning effect Effects 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 5
- 230000037396 body weight Effects 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- AMWRITDGCCNYAT-UHFFFAOYSA-L hydroxy(oxo)manganese;manganese Chemical compound [Mn].O[Mn]=O.O[Mn]=O AMWRITDGCCNYAT-UHFFFAOYSA-L 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Inorganic Chemistry (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a feed capable of relieving the symptom of manganism of chickens, as well as a preparation method and using method of the feed, and relates to the technical field of feeds, in particular to the feed capable of relieving the symptom of manganism of chickens, as well as the preparation method and using method of the feed. The preparation method comprises the following steps of adding manganese chloride to cyanobacteria crushing fluid, performing anaerobic fermentation, and adding lactobacillus acidophilus activate fluid so as to prepare fermentation fluid; and uniformly mixing the fermentation fluid with corn, millet, sorghum and bean cakes. The feed capable of relieving the symptom of manganism of chickens is fed for the chickens according to the consumption that every chicken is fed with 20-40 grams of the feed. The feed disclosed by the invention can sufficiently guarantee manganese elements required by the growth of poultry animals, including chickens and the like; on the other aspect, the symptom of the manganism of the chickens can be relieved, the growth rate of the chickens is notably increased, and the death rate of the chickens, caused by the manganism is reduced.
Description
Technical field
The present invention relates to field of feed, particularly relate to a kind of feed and the preparation and application thereof of alleviating chicken toxicity symptom.
Background technology
Manganese is one of necessary trace element of animal, has the important physiological actions such as growth promoting effects, strengthening immunity and raising animal reproduction performance.
In the feed of the poultry animals such as chicken, the content of manganese can not too high can not be too low. If shortage manganese, it is easy to cause the metabolic disturbance of carbohydrate and lipid, skeleton deformity, cessation of growth cessation, the problem such as reproductive function is abnormal.
In existing poultry cultivation process, in order to meet chicken to the demand of manganese, prevent chicken to lack manganese, feed formulation process often needs a large amount of interpolation manganese preparation, has too much of a good thing, manganese even can be caused poisoning. Also some region is polluted owing to environment is subject to manganese, is also easy to occur the phenomenon that manganese is poisoning. After manganese is poisoning, the symptom that bird produces has: the speed of growth slows down, or even mortality ratio increases.
Traditional manganese preparation, such as manganous sulfate, manganese oxide or Manganous chloride tetrahydrate etc., can destroy the VITAMIN in feed and other active substances, and absorption rate is very low, a large amount of manganese not absorbed excretes with excrement, has both wasted limited promoter manganese, brings potential manganese to environment and pollutes.
Summary of the invention
In view of the problem existing for prior art, the present invention provides a kind of and carries out the standby feed of fermentation and its preparation method, using method taking blue-green algae and Lactobacterium acidophilum as main raw material, the manganese element needed for poultry growth of animal such as guarantee chicken that on the one hand can be sufficient; On the other hand, it is possible to alleviate chicken manganese toxicity symptom, the speed of growth significantly improving chicken reduces by the poisoning mortality ratio caused of manganese.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
Alleviate a preparation method for the feed of chicken manganese toxicity symptom, comprise the following steps:
1) by blue-green algae ultrasonication, the broken liquid of blue-green algae is obtained;
2) adding manganese chloride solution in the broken liquid of blue-green algae, Manganous chloride tetrahydrate final concentration is 20-50g/L, in anaerobic condition fermentation, obtains blue algae fermentation liquid;
3) Lactobacterium acidophilum powder is placed in MRS liquid nutrient medium to activate, when OD600 value is 0.8-1.0, collects bacterium liquid, be activation solution;
4) adding in blue algae fermentation liquid that dipotassium hydrogen phosphate final concentration is 10g/L, to add magnesium sulfate final concentration be 5g/L, add sodium-chlor final concentration is 10g/L, mixed even obtains blue algae fermentation mixed solution;Accessing activation solution in blue algae fermentation mixed solution, the mass ratio of described activation solution and blue algae fermentation mixed solution is 1:10, and culture temperature is 37 DEG C, and incubation time is 48 hours, obtained fermented liquid;
5) fermented liquid is mixed with corn, millet, Chinese sorghum, soya-bean cake; The mass ratio of described fermented liquid, corn, millet, Chinese sorghum, soya-bean cake is 35:30:10:14:11, obtains feed.
The invention has the beneficial effects as follows: contriver's unexpected mixed fermentation finding that blue-green algae and Lactobacterium acidophilum carry out in feed for many years is studied can ensure that the offer of enough manganese elements can be alleviated again because manganese is poisoning, so the present invention carries out complex ferment with blue-green algae and Lactobacterium acidophilum, and have selected rational fermentation condition, further ensure the effect that feed possesses. First by blue-green algae cytoclasis, the polypeptide, amino acid and the albumen that contain in the broken liquid of blue-green algae can carry out chelating with mn ion, utilize mn ion by the metabolism of the part blue-green algae cell in the broken liquid of blue-green algae simultaneously. After Lactobacterium acidophilum activates so that it is have good vigor, with blue algae fermentation liquid mixed fermentation, on the one hand, secondly, blue algae fermentation liquid provides sufficient nutrient material for Lactobacterium acidophilum, is conducive to the fermentation of Lactobacterium acidophilum; On the other hand, the acidic substance that Lactobacterium acidophilum produces are conducive to blue-green algae to the utilization of mn ion. In fermenting process, whether the selection of various parameter is suitable important impact to fermentation results, especially, the present invention relate to the mixed fermentation of two kinds of different substancess, substratum more complicated, this also makes the selection difficulty of conditions suitable very big, and the present invention has found the most suitable culture condition and each parameter unexpectedly, proves by experiment really to have and alleviates the poisoning good result of manganese.
On the basis of technique scheme, the present invention can also do following improvement.
Further, the described ultrasonication time is 15 minutes.
Adopting the useful effect of above-mentioned further scheme to be: if the broken time is too short, content is more difficult to flow out from cell walls, is unfavorable for the sequestering action of content (such as polypeptide, amino acid and protein) with mn ion; If broken overlong time, overwhelming majority blue-green algae cell is destroyed, and affects follow-up fermenting process.
Further, step 2) in, described anaerobic fermentation conditions is: temperature is 37 DEG C, cultivates 24 hours.
The useful effect of above-mentioned further scheme is adopted to be: the fermentation being conducive to blue-green algae.
Further, the formula of described MRS liquid nutrient medium is: peptone 10g/L, extractum carnis 10g/L, yeast extract paste 5g/L, diammonium hydrogen citrate 2g/L, glucose 20g/L, the concentration of tween 80 is 1mL/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.58g/L, manganous sulfate 0.25g/L, pH are 6.2-6.6.
Further, described step 3) in, the mass ratio of described Lactobacterium acidophilum powder and MRS liquid nutrient medium is (1-3): 100.
Further, temperature during described activation is 37 DEG C, and rotating speed is 100-150rpm.
The useful effect of above-mentioned further scheme is adopted to be: the activation being conducive to Lactobacterium acidophilum.
The feed of the present invention's alleviation chicken manganese toxicity symptom that also prepared by offer aforesaid method.
The useful effect of employing such scheme is: the manganese element needed for poultry growth of animal such as guarantee chicken that on the one hand can be sufficient; On the other hand, it is possible to alleviate chicken manganese toxicity symptom.
The present invention also provides the using method of a kind of feed alleviating chicken manganese toxicity symptom, and concrete steps are: according to the forage feed chicken of every chicken consumption of 20-40 gram every day by above-mentioned alleviation chicken manganese toxicity symptom.
The useful effect of employing such scheme is: by rational feeding amount, the manganese element needed for poultry growth of animal such as guarantee chicken that on the one hand can be sufficient; On the other hand, it is possible to alleviate chicken manganese toxicity symptom.
Embodiment
Principle and feature to the present invention is described below, and example, only for explaining the present invention, is not intended to limit the scope of the present invention.
In the embodiment of the present invention, Lactobacterium acidophilum powder is purchased from Shandong Zhong Kejia hundred million biotechnology company limited.
In each embodiment, MRS liquid nutrient medium is lactic-acid-bacterium substratum, and its formula is: peptone 10g/L, extractum carnis 10g/L, yeast extract paste 5g/L, diammonium hydrogen citrate 2g/L, glucose 20g/L, the concentration of tween 80 is 1mL/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.58g/L, manganous sulfate 0.25g/L, pH are 6.2-6.6. 121 DEG C of sterilizings use for 15 minutes.
Embodiment 1
Alleviate a preparation method for the feed of chicken manganese toxicity symptom, comprise the following steps:
1) by the blue-green algae ultrasonication of fresh salvaging, the broken liquid of blue-green algae is obtained; The described ultrasonication time is 15 minutes, it may also be useful to the output rating of Ultrasonic Cell Disruptor be 1 kilowatt;
2) adding manganese chloride solution in the broken liquid of blue-green algae, Manganous chloride tetrahydrate final concentration is 20g/L, and in anaerobic condition fermentation, temperature is 37 DEG C, cultivates 24 hours, obtains blue algae fermentation liquid;
3) Lactobacterium acidophilum powder being placed in MRS liquid nutrient medium to activate, the mass ratio of described Lactobacterium acidophilum powder and MRS liquid nutrient medium is 1:100, and temperature is 37 DEG C, rotating speed is 100rpm, when OD600 value is 0.8, collect bacterium liquid, it is activation solution;
4) adding in blue algae fermentation liquid that dipotassium hydrogen phosphate final concentration is 10g/L, to add magnesium sulfate final concentration be 5g/L, add sodium-chlor final concentration is 10g/L, mixed even obtains blue algae fermentation mixed solution; Accessing activation solution in blue algae fermentation mixed solution, the mass ratio of described activation solution and blue algae fermentation mixed solution is 1:10, and culture temperature is 37 DEG C, and incubation time is 48 hours, obtained fermented liquid;
5) fermented liquid is mixed with corn, millet, Chinese sorghum, soya-bean cake; The mass ratio of described fermented liquid, corn, millet, Chinese sorghum, soya-bean cake is 35:30:10:14:11, obtains feed.
Embodiment 2
Alleviate a preparation method for the feed of chicken manganese toxicity symptom, comprise the following steps:
1) by the blue-green algae ultrasonication of fresh salvaging, the broken liquid of blue-green algae is obtained; The described ultrasonication time is 15 minutes, it may also be useful to the output rating of Ultrasonic Cell Disruptor be 1 kilowatt;
2) adding manganese chloride solution in the broken liquid of blue-green algae, Manganous chloride tetrahydrate final concentration is 30g/L, and in anaerobic condition fermentation, temperature is 37 DEG C, cultivates 24 hours, obtains blue algae fermentation liquid;
3) Lactobacterium acidophilum powder being placed in MRS liquid nutrient medium to activate, the mass ratio of described Lactobacterium acidophilum powder and MRS liquid nutrient medium is 2:100, and temperature is 37 DEG C, rotating speed is 120rpm, when OD600 value is 0.9, collect bacterium liquid, it is activation solution;
4) adding in blue algae fermentation liquid that dipotassium hydrogen phosphate final concentration is 10g/L, to add magnesium sulfate final concentration be 5g/L, add sodium-chlor final concentration is 10g/L, mixed even obtains blue algae fermentation mixed solution; Accessing activation solution in blue algae fermentation mixed solution, the mass ratio of described activation solution and blue algae fermentation mixed solution is 1:10, and culture temperature is 37 DEG C, and incubation time is 48 hours, obtained fermented liquid;
5) fermented liquid is mixed with corn, millet, Chinese sorghum, soya-bean cake; The mass ratio of described fermented liquid, corn, millet, Chinese sorghum, soya-bean cake is 35:30:10:14:11, obtains feed.
Embodiment 3
Alleviate a preparation method for the feed of chicken manganese toxicity symptom, comprise the following steps:
1) by the blue-green algae ultrasonication of fresh salvaging, the broken liquid of blue-green algae is obtained; The described ultrasonication time is 15 minutes, it may also be useful to the output rating of Ultrasonic Cell Disruptor be 1 kilowatt;
2) adding manganese chloride solution in the broken liquid of blue-green algae, Manganous chloride tetrahydrate final concentration is 50g/L, and in anaerobic condition fermentation, temperature is 37 DEG C, cultivates 24 hours, obtains blue algae fermentation liquid;
3) Lactobacterium acidophilum powder being placed in MRS liquid nutrient medium to activate, the mass ratio of described Lactobacterium acidophilum powder and MRS liquid nutrient medium is 3:100, and temperature is 37 DEG C, rotating speed is 150rpm, when OD600 value is 1.0, collect bacterium liquid, it is activation solution;
4) adding in blue algae fermentation liquid that dipotassium hydrogen phosphate final concentration is 10g/L, to add magnesium sulfate final concentration be 5g/L, add sodium-chlor final concentration is 10g/L, mixed even obtains blue algae fermentation mixed solution; Accessing activation solution in blue algae fermentation mixed solution, the mass ratio of described activation solution and blue algae fermentation mixed solution is 1:10, and culture temperature is 37 DEG C, and incubation time is 48 hours, obtained fermented liquid;
5) fermented liquid is mixed with corn, millet, Chinese sorghum, soya-bean cake; The mass ratio of described fermented liquid, corn, millet, Chinese sorghum, soya-bean cake is 35:30:10:14:11, obtains feed.
Embodiment 4
Step 3 by embodiment 3) in Lactobacterium acidophilum powder change the water of equivalent into, all the other are all identical with embodiment 3.
Embodiment 5
Step 1 by embodiment 3) blue-green algae replace to the water of equivalent, all the other are all identical with embodiment 3.
Embodiment 6
Step 1 by embodiment 3) blue-green algae and step 3) in Lactobacterium acidophilum powder all change the water of equivalent into, all the other are all identical with embodiment 3.
Embodiment 7
Step 1 by embodiment 3) blue-green algae, step 3) in Lactobacterium acidophilum, step 2) in manganese chloride solution all change the water of equivalent into, all the other are identical with embodiment 3.
Comparative example
Getting the blue cock 700 in healthy sea age on the 1st respectively, be divided into 7 groups at random, often organize 100, routine was raised to age on the 100th, and every chicken feeds 40 grams of feeds every day.
First group of feed fed embodiment 1 and prepare; The 2nd group of feed fed embodiment 2 and prepare; The 3rd group of feed fed embodiment 3 and prepare; The 4th group of feed fed embodiment 4 and prepare; The 5th group of feed fed embodiment 5 and prepare; The 6th group of feed fed embodiment 6 and prepare; The 7th group of feed fed embodiment 7 and prepare.
The body weight of the blue cock in the every only sea of record, carries out statistical analysis respectively, calculates the mean body weight of the often blue cock in group sea, and statistics is in table 1. Recording the mortality ratio from the blue cock in the sea between the length of time in age to 100 day on the 1st, concrete outcome is in table 2 simultaneously.
The body weight of first group, table 1 to the 6th group
Data from table 1, it may be seen that prepared by the present invention feed can significantly promote growing of the blue cock in sea, are conducive to the increase of the body weight of the blue cock in sea.
The mortality ratio of the blue cock in the sea between table 21 day length of time in age to 100 day
| First group | 2nd group | 3rd group | 4th group | 5th group | 6th group | 7th group | |
| Mortality ratio (%) | 8 | 7 | 6 | 23 | 19 | 42 | 31 |
As can be seen from Table 2, the feed that prepared by the present invention can obviously reduce the mortality ratio of the blue cock in sea.
The foregoing is only the better embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. alleviate the preparation method of the feed of chicken manganese toxicity symptom for one kind, it is characterised in that, comprise the following steps:
1) by blue-green algae ultrasonication, the broken liquid of blue-green algae is obtained;
2) adding manganese chloride solution in the broken liquid of blue-green algae, Manganous chloride tetrahydrate final concentration is 20-50g/L, in anaerobic condition fermentation, obtains blue algae fermentation liquid;
3) Lactobacterium acidophilum powder is placed in MRS liquid nutrient medium to activate, treats OD600When value is for 0.8-1.0, collects bacterium liquid, it is activation solution;
4) adding in blue algae fermentation liquid that dipotassium hydrogen phosphate final concentration is 10g/L, to add magnesium sulfate final concentration be 5g/L, add sodium-chlor final concentration is 10g/L, mixed even obtains blue algae fermentation mixed solution; Accessing activation solution in blue algae fermentation mixed solution, the mass ratio of described activation solution and blue algae fermentation mixed solution is 1:10, and culture temperature is 37 DEG C, and incubation time is 48 hours, obtained fermented liquid;
5) fermented liquid is mixed with corn, millet, Chinese sorghum, soya-bean cake; The mass ratio of described fermented liquid, corn, millet, Chinese sorghum, soya-bean cake is 35:30:10:14:11, obtains feed.
2. the preparation method of a kind of feed alleviating chicken manganese toxicity symptom according to claim 1, it is characterised in that, the described ultrasonication time is 15 minutes.
3. the preparation method of a kind of feed alleviating chicken manganese toxicity symptom according to claim 1, it is characterised in that, step 2) in, described anaerobic fermentation conditions is: temperature is 37 DEG C, cultivates 24 hours.
4. the preparation method of a kind of feed alleviating chicken manganese toxicity symptom according to claim 1, it is characterised in that, the formula of described MRS liquid nutrient medium is: peptone 10g/L, extractum carnis 10g/L, yeast extract paste 5g/L, diammonium hydrogen citrate 2g/L, glucose 20g/L, the concentration of tween 80 is 1mL/L, sodium acetate 5g/L, dipotassium hydrogen phosphate 2g/L, magnesium sulfate 0.58g/L, manganous sulfate 0.25g/L, pH are 6.2-6.6.
5. the preparation method of a kind of feed alleviating chicken manganese toxicity symptom according to claim 1, it is characterised in that, described step 3) in, the mass ratio of described Lactobacterium acidophilum powder and MRS liquid nutrient medium is (1-3): 100.
6. the preparation method of a kind of feed alleviating chicken manganese toxicity symptom according to claim 5, it is characterised in that, step 3) in, temperature during described activation is 37 DEG C, and rotating speed is 100-150rpm.
7. the feed of alleviation chicken manganese toxicity symptom prepared by claim 1 to the 6 described method of arbitrary item.
8. described in claim 7, alleviate the using method of the feed of chicken manganese toxicity symptom, it is characterised in that, according to every chicken consumption of 20-40 gram every day by the feed of alleviation chicken manganese toxicity symptom for chicken feed.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610004137.3A CN105661084A (en) | 2016-01-05 | 2016-01-05 | Feed capable of relieving symptom of manganism of chickens, as well as preparation method and using method of feed |
| CN201910039783.7A CN109549020A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
| CN201910039782.2A CN109549019A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
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| CN201610004137.3A CN105661084A (en) | 2016-01-05 | 2016-01-05 | Feed capable of relieving symptom of manganism of chickens, as well as preparation method and using method of feed |
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| CN201910039782.2A Division CN109549019A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
| CN201910039783.7A Division CN109549020A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
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| CN201910039782.2A Withdrawn CN109549019A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
| CN201910039783.7A Withdrawn CN109549020A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
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| CN201910039783.7A Withdrawn CN109549020A (en) | 2016-01-05 | 2016-01-05 | A kind of preparation method and application method of feed that alleviating chicken manganese poisoning symptom |
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| US11882453B2 (en) | 2018-02-21 | 2024-01-23 | Telefonaktiebolaget Lm Ericsson (Publ) | Fraudelent subscription detection |
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| US11882453B2 (en) | 2018-02-21 | 2024-01-23 | Telefonaktiebolaget Lm Ericsson (Publ) | Fraudelent subscription detection |
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| CN109549020A (en) | 2019-04-02 |
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