CN105660526B - A kind of method for collecting Sacalis bealei ovum - Google Patents
A kind of method for collecting Sacalis bealei ovum Download PDFInfo
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- CN105660526B CN105660526B CN201610012800.4A CN201610012800A CN105660526B CN 105660526 B CN105660526 B CN 105660526B CN 201610012800 A CN201610012800 A CN 201610012800A CN 105660526 B CN105660526 B CN 105660526B
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- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 112
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 112
- 210000004681 ovum Anatomy 0.000 title claims abstract description 112
- 238000000034 method Methods 0.000 title claims abstract description 27
- 241000270708 Testudinidae Species 0.000 claims abstract description 116
- 235000019354 vermiculite Nutrition 0.000 claims description 40
- 229910052902 vermiculite Inorganic materials 0.000 claims description 36
- 239000010455 vermiculite Substances 0.000 claims description 36
- 210000004185 liver Anatomy 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 210000000481 breast Anatomy 0.000 claims description 14
- 241001237160 Kallima inachus Species 0.000 claims description 10
- 230000012447 hatching Effects 0.000 claims description 9
- 238000007689 inspection Methods 0.000 claims description 7
- 230000018109 developmental process Effects 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 238000009313 farming Methods 0.000 abstract description 5
- 230000017448 oviposition Effects 0.000 abstract description 4
- 230000001850 reproductive effect Effects 0.000 abstract description 4
- 230000006399 behavior Effects 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000008092 positive effect Effects 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 241000270711 Malaclemys terrapin Species 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000270605 Chelonia Species 0.000 description 1
- 241000270693 Chinemys Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241001194892 Sacalia bealei Species 0.000 description 1
- 241001453636 Salvinia Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008011 embryonic death Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Housing For Livestock And Birds (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of method for collecting Sacalis bealei ovum.The present invention is based on culture experiment research for many years, there is provided a kind of method for collecting Sacalis bealei ovum, compared with prior art, this method takes into full account the ecological biology features such as spawning period of Sacalis bealei, oviposition behavior, egg-laying environment demand；With this method, can effectively solve the problems, such as the tortoise ovum collecting during Sacalis bealei artificial farming, under conditions of being substantially reduced to the interference of female tortoise, significantly improve the efficiency of tortoise ovum collecting, ensure that more than 80% tortoise ovum being capable of safe collection, the loss of tortoise ovum is significantly reduced, improves reproductive efficiency；This method positive effect, easily operation, saves space resources, saves human cost, is advantageous to popularization and application.
Description
Technical field：
The invention belongs to aquatic economic animals cultural technique field, and in particular to a kind of side of artificially collecting of Sacalis bealei ovum
Method.
Background technology：
Sacalis bealei (Sacalia bealei) is under the jurisdiction of Chelonia, damp Testudinidae, eye spot Chinemys, is Chinese unique wheat, main
It is distributed in the ground such as Guangdong, Guangxi, Jiangxi, Fujian, Guizhou, Hong Kong.Sacalis bealei has important edible, medicinal and views and admires valency
Value, market economy value is higher, and current market price is up to 300-4000 members/kg.For a long time, due to excessively catching and trade
Easily, wild resource drastically declines, and causes Sacalis bealei to turn into endangered species.Carry out Sacalis bealei captive, not only can
Effectively expand population quantity, avoid species extinction, and be advantageously implemented the sustainable use of Animal resources.As a kind of excellent
Terrapin breed variety, in recent years Sacalis bealei cultivation increasingly paid close attention to by culture fishery.
However, during Sacalis bealei captive, still suffer from many technical problems have it is to be solved.Wherein, tortoise
The collection problem of ovum is one of main bugbear faced during Sacalis bealei chartering cost.Under the conditions of artificial farming, eye spot
Terrapin generally lays eggs in water, causes embryonic death, it is impossible to normal development；Or nest can not be normally dug, lay eggs on ground, this
Sample easily causes ovum to be eaten up or destroyed by animal.At present, during Sacalis bealei artificial farming, the collection problem of tortoise ovum is still
It is one of important restriction factor for influenceing Sacalis bealei healthy aquaculture.
The content of the invention：
It is an object of the invention to provide a kind of method for collecting Sacalis bealei ovum, this method can effectively solve Sacalis bealei
Collection problem in breeding process to ovum, under conditions of being substantially reduced to the interference of female tortoise, significantly improve the effect of tortoise ovum collecting
Rate, the loss of tortoise ovum is reduced, improve reproductive efficiency, solid technical support is provided for the healthy aquaculture of Sacalis bealei.
The method of the collection Sacalis bealei ovum of the present invention, it is characterised in that comprise the following steps：
A, female tortoise spawning situation inspection and tortoise ovum collecting time：In the first tenday period of a month in early April to May, periodically examined using medical X-ray
The bosom ovate condition of female tortoise is surveyed, the development degree of bosom ovum quantity and ovum to tortoise judges, if finding female tortoise bosom ovum, that is, starts tortoise
The collection work of ovum；The first tenday period of a month in May to early June is Sacalis bealei egg production peak time, therefore this period is the pass of tortoise ovum collecting
Key period；
B, the management during the setting and collection of tortoise ovum collecting case：Described tortoise ovum collecting case includes casing and shadow shield case
Lid, several holes are respectively provided with four perisporiums of casing, are covered with vermiculite layers in the bottom of casing, in vermiculite layers again covered with liver moss or
Dead leaf layer, this tortoise ovum collecting case is positioned over interior, keeps indoor quiet and good ventilation condition, sprayed on liver moss or dead leaf
Water, liver moss or dead leaf is kept moist, the female tortoise of a bosom ovum is then put in tortoise ovum collecting case, covers shadow shield case lid, is protected
Rather dark in case, tortoise ovum collecting case of inspection per 1-2 days is held, opens vermiculite, inspection spawning situation, while remove the excrement of tortoise
Just, tortoise is taken out to be positioned in bucket and fed, after tortoise feeds, tortoise is put back in tortoise ovum collecting case；
C, tortoise ovum collecting：When finding tortoise ovum in tortoise ovum collecting case, marked in the surface of ovum with oil pen, by tortoise
Ovum is gently taken out and (can not rock or shake), and the position for keeping making marks is in surface, collection tortoise ovum.
It is preferred that the tortoise ovum gathered can be positioned in preprepared hatching box, 5-10cm is set in hatching box
Vermiculite layers, contain water in vermiculite layers, tortoise ovum is lain against in vermiculite layers, then covers the thick vermiculites of 1-2cm again on tortoise ovum,
Hatching house is put into be hatched.
It is preferred that described step B casing is plastic box, its color is black, dark-brown, grey black or navy blue, is grown
Wide a height of 40cm × 30cm × 20cm, 6-12 diameter 2cm circle is set below the top edge of the wall of plastic box four at 3-4cm
Hole, circular hole are evenly distributed in surrounding.
It is preferred that the thickness of described step B vermiculite layers is 5cm, the thickness of described liver moss or dead leaf layer is 5-10cm.
It is preferred that the described step B female tortoise that a bosom ovum is put in tortoise ovum collecting case, multiple tortoise ovum collecting casees can stack
Into multilayer, to improve space availability ratio.
It is preferred that described take out tortoise is positioned in bucket that to carry out feeding be to take out tortoise to be positioned over 1 hour left side in bucket
Right to be fed, water depth is advisable with flooding 2/3rds of tortoise body.
It is preferred that setting 5-10cm vermiculite layers in described hatching box, contain water in vermiculite layers, its water and vermiculite mass ratio
For 0.9：1.
It is preferred that described vermiculite is all by 0.5 ‰ liquor potassic permanganate soaking disinfection of vermiculite mass fraction 30 minutes, so
Cleaning is carried out with water afterwards and remove potassium permanganate, then dry or dry the vermiculite after being sterilized.
Described liver moss and dead leaf, liver moss can be withered liver moss, or fresh liver moss；The dead leaf of selection for
It is withered and yellow but there is no decomposed leaves, the leaf of bamboo or blade of grass, it is clearly clean with water to liver moss or dead leaf.
Described shadow shield case lid, is made of plank or plastic plate, and size is slightly larger than plastic box upper opening.
Described multiple tortoise ovum collecting casees can be superimposed as multilayer, refer to stack up in 2-5 tortoise ovum collecting case, so
Space availability ratio can be improved.
The present invention is based on culture experiment research for many years, there is provided a kind of method for collecting Sacalis bealei ovum, with existing skill
Art is compared, and this method takes into full account that the ecological biologies such as spawning period of Sacalis bealei, oviposition behavior, egg-laying environment demand are special
Point；With this method, can effectively solve the problems, such as the tortoise ovum collecting during Sacalis bealei artificial farming, be substantially reduced to female
Under conditions of tortoise interference, significantly improve the efficiency of tortoise ovum collecting, ensure more than 80% tortoise ovum can safe collection, significantly subtract
Lack the loss of tortoise ovum, improve reproductive efficiency；This method positive effect, easily operation, save space resources, save manpower into
This, is advantageous to popularization and application.
Brief description of the drawings：
Fig. 1 is the structural representation of the tortoise ovum collecting case of the present invention；
Fig. 2 is the schematic diagram that multiple tortoise ovum collecting casees are stacked together；
Wherein 1, casing；2nd, shadow shield case lid；3rd, hole；4th, vermiculite layers；5th, liver moss layer.
Embodiment：
Following examples are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1：
First, tortoise ovum collecting case
As shown in figure 1, described tortoise ovum collecting case includes casing 1 and shadow shield case lid 2, described casing is plastic box,
Its color is black, a height of 40cm × 30cm of length and width × 20cm, sets 12 at 3-4cm below the top edge of the wall of plastic box four
Diameter 2cm circular hole 3,3 a diameter of 2cm circular hole is respectively provided with each perisporium, the thick vermiculites of 5cm are covered with the bottom of casing
Layer 4, again covered with liver moss layer 5 thick 5-10cm in vermiculite layers, the liver moss of liver moss layer is tongue that is fresh, being cleaned up with clear water
Moss.Described shadow shield case lid is made of plank, and size is slightly larger than plastic box upper opening, and the length of shadow shield and wide ratio are moulded
The length of hopper upper opening and wide 1cm more respectively are advisable, and shading plate thickness 2-5mm is advisable.
2nd, the method for collecting Sacalis bealei ovum
A, female tortoise spawning situation inspection and tortoise ovum collecting time：In the first tenday period of a month in early April to May, medical X was used every 15 days
Light detects the bosom ovate condition of female tortoise, and the development degree of bosom ovum quantity and ovum to tortoise judges, if finding female tortoise bosom ovum, that is, opens
The collection work of beginning tortoise ovum；Sacalis bealei is generally laid eggs during the 5-6 months, therefore when this period is the key of tortoise ovum collecting
Phase；
B, the management during the setting and collection of tortoise ovum collecting case：Tortoise ovum collecting case is positioned over interior, keeps indoor peace
Quiet and good ventilation condition, is sprayed water on liver moss, liver moss is kept moist, and one is then put in tortoise ovum collecting case and cherishes ovum
Female tortoise, shadow shield case lid is covered, keep rather dark in case, as shown in Fig. 2 multiple tortoise ovum collecting casees can be superimposed as multilayer,
To improve space availability ratio, a tortoise ovum collecting case was checked per 1-2 days, opens vermiculite, checks spawning situation, while remove tortoise
Excrement, tortoise is taken out to be positioned over 1 hour or so in bucket being fed, water depth is to flood 2/3rds of tortoise body
Preferably, after tortoise feeds, tortoise is put back in tortoise ovum collecting case；
C, tortoise ovum collecting：When finding tortoise ovum in tortoise ovum collecting case, marked in the surface of ovum with oil pen, by tortoise
Ovum is gently taken out and (can not rock or shake), keeps the position that makes marks that tortoise ovum is positioned over into preprepared in surface
Hatch in box；Hatch the vermiculite layers that 5-10cm is set in box, water is contained in vermiculite layers, vermiculite is with water quality than 0.9：1 mixing, tortoise
Ovum is lain against in vermiculite layers, is then covered the thick vermiculites of 1-2cm again on tortoise ovum, is put into hatching house and is hatched.
Above-mentioned vermiculite is all to divide the liquor potassic permanganate soaking disinfection 30 of vermiculite mass fraction 0.5 ‰ that collection is returned
Clock, then carry out cleaning with water and remove potassium permanganate, then dry or dry, the vermiculite after being sterilized reuses.
According to the present embodiment method collect Sacalis bealei ovum, can effectively solve Sacalis bealei artificial farming during
Tortoise ovum collecting problem, under conditions of being substantially reduced female tortoise is disturbed, significantly improve the efficiency of tortoise ovum collecting, more than 80%
Tortoise ovum can safe collection, significantly reduce the loss of tortoise ovum, improve reproductive efficiency.
Claims (8)
- A kind of 1. method for collecting Sacalis bealei ovum, it is characterised in that comprise the following steps：A, female tortoise spawning situation inspection and tortoise ovum collecting time：In the first tenday period of a month in early April to May, periodically detected using medical X-ray female The bosom ovate condition of tortoise, the development degree of bosom ovum quantity and ovum to tortoise judge, if finding female tortoise bosom ovum, that is, start tortoise ovum Collection work；B, the management during the setting and collection of tortoise ovum collecting case：Described tortoise ovum collecting case includes casing and shadow shield case lid, Several holes are respectively provided with four perisporiums of casing, are covered with vermiculite layers in the bottom of casing, again covered with liver moss or withered in vermiculite layers Leaf layer, this tortoise ovum collecting case is positioned over interior, keeps indoor quiet and good ventilation condition, sprayed on liver moss or dead leaf Water, liver moss or dead leaf is kept moist, the female tortoise of a bosom ovum is then put in tortoise ovum collecting case, covers shadow shield case lid, is protected Rather dark in case, tortoise ovum collecting case of inspection per 1-2 days is held, opens vermiculite, inspection spawning situation, while remove the excrement of tortoise Just, tortoise is taken out to be positioned in bucket and fed, after tortoise feeds, tortoise is put back in tortoise ovum collecting case；C, tortoise ovum collecting：When finding tortoise ovum in tortoise ovum collecting case, marked directly over ovum, tortoise ovum gently taken out, The position for keeping making marks is in surface, collection tortoise ovum.
- 2. the method according to claim 1 for collecting Sacalis bealei ovum, it is characterised in that be positioned over the tortoise ovum gathered In preprepared hatching box, 5-10cm vermiculite layers are set in hatching box, contain water in vermiculite layers, tortoise ovum lies against vermiculite On layer, then cover the thick vermiculites of 1-2cm again on tortoise ovum, be put into hatching house and hatched.
- 3. the method according to claim 1 or 2 for collecting Sacalis bealei ovum, it is characterised in that described step B casing For plastic box, its color is black, dark-brown, grey black or navy blue, a height of 40cm × 30cm of length and width × 20cm, in plastic box 6-12 diameter 2cm circular hole is set below the top edge of four walls at 3-4cm, and circular hole is evenly distributed in surrounding.
- 4. the method according to claim 1 or 2 for collecting Sacalis bealei ovum, it is characterised in that described step B vermiculite The thickness of layer is 5cm, and the thickness of described liver moss or dead leaf layer is 5-10cm.
- 5. it is according to claim 1 or 2 collect Sacalis bealei ovum method, it is characterised in that described step B in tortoise The female tortoise of a bosom ovum is put in ovum collecting case, multiple tortoise ovum collecting casees stack into multilayer.
- 6. the method according to claim 1 or 2 for collecting Sacalis bealei ovum, it is characterised in that described take out tortoise is put It is to take out tortoise to be positioned in bucket to be fed to be placed in bucket and carry out feeding, water depth with flood the three of tortoise body/ Two.
- 7. the method according to claim 2 for collecting Sacalis bealei ovum, it is characterised in that 5- is set in described hatching box 10cm vermiculite layers, contain water in vermiculite layers, its water is 0.9 with vermiculite mass ratio：1.
- 8. it is according to claim 1 or 2 collect Sacalis bealei ovum method, it is characterised in that described vermiculite be all by Vermiculite 0.5 ‰ liquor potassic permanganate soaking disinfection of mass fraction 30 minutes, then carries out cleaning with water and removes potassium permanganate, then Dry or dry, the vermiculite after being sterilized.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106417120A (en) * | 2016-12-14 | 2017-02-22 | 中国电建集团贵阳勘测设计研究院有限公司 | Artificial fish spawning bed |
| CN107318720B (en) * | 2017-08-02 | 2023-04-14 | 中国水产科学研究院南海水产研究所 | Clown fish hatching egg collection device |
| CN109548745A (en) * | 2018-12-07 | 2019-04-02 | 广东省生物资源应用研究所 | A kind of method of low temperature induction tortoise ovum diapause |
| CN112011601A (en) * | 2020-08-19 | 2020-12-01 | 广东省生物资源应用研究所 | Identification primer, kit and identification method for sex-related microsatellite markers of scarab beetles |
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| CN102283170A (en) * | 2011-07-04 | 2011-12-21 | 安徽省农业科学院水产研究所 | Low-carbon ecological breeding system for turtles |
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