CN105658813B - 包括选择和富集步骤的染色体构象捕获方法 - Google Patents

包括选择和富集步骤的染色体构象捕获方法 Download PDF

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CN105658813B
CN105658813B CN201480049002.8A CN201480049002A CN105658813B CN 105658813 B CN105658813 B CN 105658813B CN 201480049002 A CN201480049002 A CN 201480049002A CN 105658813 B CN105658813 B CN 105658813B
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nucleic acid
dna
pcr
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CN105658813A (zh
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P.弗拉泽
C.奥斯本
S.舍恩费尔德
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Babraham Institute
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/6855—Ligating adaptors
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    • C12Q2600/00—Oligonucleotides characterized by their use
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CN201480049002.8A 2013-09-05 2014-09-04 包括选择和富集步骤的染色体构象捕获方法 Active CN105658813B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1315777.1A GB2517936B (en) 2013-09-05 2013-09-05 Chromosome conformation capture method including selection and enrichment steps
GB1315777.1 2013-09-05
PCT/GB2014/052664 WO2015033134A1 (en) 2013-09-05 2014-09-04 Chromosome conformation capture method including selection and enrichment steps

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CN105658813A CN105658813A (zh) 2016-06-08
CN105658813B true CN105658813B (zh) 2021-01-05

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US (1) US20160194713A1 (enExample)
EP (1) EP3041951B1 (enExample)
JP (1) JP6514210B2 (enExample)
CN (1) CN105658813B (enExample)
AU (2) AU2014316880A1 (enExample)
CA (1) CA2922244C (enExample)
GB (1) GB2517936B (enExample)
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Families Citing this family (12)

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WO2017066907A1 (zh) * 2015-10-19 2017-04-27 安诺优达基因科技(北京)有限公司 一种构建高可利用数据率的Hi-C文库的方法
GB201518843D0 (en) * 2015-10-23 2015-12-09 Isis Innovation Method of analysing DNA sequences
CN107119120A (zh) * 2017-05-04 2017-09-01 河海大学常州校区 一种基于染色质3d构象技术的关键作用分子检测方法
CN108300767B (zh) * 2017-10-27 2021-08-20 清华大学 一种核酸复合体中核酸区段相互作用的分析方法
CN108220394B (zh) * 2018-01-05 2021-03-23 清华大学 基因调控性染色质相互作用的鉴定方法、系统及其应用
CN109448783B (zh) * 2018-08-07 2022-05-13 清华大学 一种染色质拓扑结构域边界的分析方法
EP3844288A1 (en) 2018-10-01 2021-07-07 Lonza Ltd. Ssi cells with predictable and stable transgene expression and methods of formation
US20220213546A1 (en) * 2019-05-22 2022-07-07 Oxford Nanopore Technologies Plc Protocol for detecting interactions within one or more dna molecules within a cell
GB201914325D0 (en) * 2019-10-04 2019-11-20 Babraham Inst Novel meethod
CN114729351A (zh) * 2019-11-15 2022-07-08 相位基因组公司 从组织样品进行染色体构象捕获
CN115335519A (zh) * 2020-03-31 2022-11-11 凯杰有限公司 从固定的生物样品纯化核酸
GB202319199D0 (en) 2023-12-14 2024-01-31 Enhanc3D Genomics Ltd Conformation capture methods

Citations (4)

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Publication number Priority date Publication date Assignee Title
CN101688237A (zh) * 2007-01-11 2010-03-31 伊拉兹马斯大学医疗中心 环状染色体构象捕获（4c）
WO2012108864A1 (en) * 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
WO2012159025A2 (en) * 2011-05-18 2012-11-22 Life Technologies Corporation Chromosome conformation analysis
CN103180459A (zh) * 2010-07-09 2013-06-26 赛尔冉迪思股份有限公司 3-d目的基因组区域的测序策略

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PL1899488T3 (pl) * 2005-07-04 2016-03-31 Erasmus Univ Medical Center Test uchwytu konformacji chromosomu na czipie (4C)
EP2245198A1 (en) * 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US9434985B2 (en) * 2008-09-25 2016-09-06 University Of Massachusetts Methods of identifying interactions between genomic loci
JP5413924B2 (ja) * 2009-08-21 2014-02-12 国立大学法人大阪大学 特定ゲノム領域の単離方法
WO2012061600A1 (en) * 2010-11-05 2012-05-10 The Broad Institute, Inc. Hybrid selection using genome-wide baits for selective genome enrichment in mixed samples
US8663924B2 (en) * 2011-11-30 2014-03-04 Agilent Technologies, Inc. Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101688237A (zh) * 2007-01-11 2010-03-31 伊拉兹马斯大学医疗中心 环状染色体构象捕获（4c）
CN103180459A (zh) * 2010-07-09 2013-06-26 赛尔冉迪思股份有限公司 3-d目的基因组区域的测序策略
WO2012108864A1 (en) * 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
WO2012159025A2 (en) * 2011-05-18 2012-11-22 Life Technologies Corporation Chromosome conformation analysis

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CA2922244C (en) 2023-01-24
AU2020280999B2 (en) 2023-02-02
CN105658813A (zh) 2016-06-08
GB2517936B (en) 2016-10-19
EP3041951B1 (en) 2018-11-28
AU2020280999A1 (en) 2021-01-07
EP3041951A1 (en) 2016-07-13
GB201315777D0 (en) 2013-10-23
GB2517936A (en) 2015-03-11
AU2014316880A1 (en) 2016-03-10
WO2015033134A1 (en) 2015-03-12
US20160194713A1 (en) 2016-07-07
CA2922244A1 (en) 2015-03-12
JP6514210B2 (ja) 2019-05-22
JP2016533758A (ja) 2016-11-04

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