CN105638647A - Preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel - Google Patents
Preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel Download PDFInfo
- Publication number
- CN105638647A CN105638647A CN201610010233.9A CN201610010233A CN105638647A CN 105638647 A CN105638647 A CN 105638647A CN 201610010233 A CN201610010233 A CN 201610010233A CN 105638647 A CN105638647 A CN 105638647A
- Authority
- CN
- China
- Prior art keywords
- concentration
- hormone
- naa
- preparation
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 title abstract 7
- 239000012224 working solution Substances 0.000 claims abstract description 15
- 239000010413 mother solution Substances 0.000 claims abstract description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 5
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 5
- 239000000661 sodium alginate Substances 0.000 claims abstract description 5
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 5
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 5
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 5
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 5
- 239000005556 hormone Substances 0.000 claims description 23
- 229940088597 hormone Drugs 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000012452 mother liquor Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000007865 diluting Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 description 1
- ZMZGFLUUZLELNE-UHFFFAOYSA-N 2,3,5-triiodobenzoic acid Chemical compound OC(=O)C1=CC(I)=CC(I)=C1I ZMZGFLUUZLELNE-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 239000005976 Ethephon Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel and belongs to the field of plant biotechnology. The preparation method comprises the following step: firstly, preparing 1mg/mL NAA mother solution; diluting the mother solution into 100mg/L middle-concentration NAA working solution; and preparing middle-concentration NAA gel confining liquid containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% from the working solution above.
Description
Technical field
The compound method of a kind of middle concentration NAA gel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle branch abundance short, used and can keep the plurality of advantages such as parent's merit. Usually can use different types of hormone during cottage propagation, as NAA, IBA, NAA, 2,4-D, GA3, 6-BA, KT, TDZ, TIBA, ethephon, B9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant ahead of time at body or in vitro or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., plays important purposes in agricultural production. But, the branch being difficult to break up in vitro breeding or rooting process, due to the loss of long moisture or nutrient substance, is extremely easily caused death. And existing employing urges the method that root lures bud to bloom to have the shortcomings such as technical step is more, cost is high, unstable, on producing, effect is unsatisfactory. Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
The compound method of a kind of middle concentration NAA gel, it is characterised in that including hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother solution of 1mg/mL;
2. working solution preparation: above-mentioned mother solution is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working solution that concentration is 100mg/L;
3. hormone gel preparation: accurately weigh 1g sodium carboxymethyl cellulose and 8g sodium alginate, dissolves standby with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
It is an advantage of the current invention that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethyl cellulose and sodium alginate are gel carrier, are configured to containing gel solution dissimilar, concentration hormone. During use, branch, flower it is soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, thin film after gel drying, can be quickly formed, reaching to reduce moisture evaporation, be continuously applied hormone, the feature such as easy and simple to handle.
Detailed description of the invention:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
The compound method of a kind of middle concentration NAA gel, it is characterised in that including hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother solution of 1mg/mL;
2. working solution preparation: above-mentioned mother solution is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working solution that concentration is 100mg/L;
3. hormone gel preparation: accurately weigh 1g sodium carboxymethyl cellulose and 8g sodium alginate, dissolves standby with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
Claims (1)
1. the compound method of concentration NAA gel in a kind, it is characterised in that including hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother solution of 1mg/mL;
2. working solution preparation: above-mentioned mother solution is diluted to, according to the ratio of volume ratio 1:10, the middle concentration hormone working solution that concentration is 100mg/L;
3. hormone gel preparation: accurately weigh 1g sodium carboxymethyl cellulose and 8g sodium alginate, dissolves standby with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610010233.9A CN105638647A (en) | 2016-01-08 | 2016-01-08 | Preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610010233.9A CN105638647A (en) | 2016-01-08 | 2016-01-08 | Preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105638647A true CN105638647A (en) | 2016-06-08 |
Family
ID=56490685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610010233.9A Pending CN105638647A (en) | 2016-01-08 | 2016-01-08 | Preparation method of middle-concentration NAA (Naphthalene Acetic Acid) gel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105638647A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
| CN105010318A (en) * | 2015-08-25 | 2015-11-04 | 钱夕华 | Preparation method of low-concentration NAA (naphthalene acetic acid) gel |
| CN105052928A (en) * | 2015-08-25 | 2015-11-18 | 钱夕华 | Preparing method for high-concentration NAA gel |
-
2016
- 2016-01-08 CN CN201610010233.9A patent/CN105638647A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
| CN105010318A (en) * | 2015-08-25 | 2015-11-04 | 钱夕华 | Preparation method of low-concentration NAA (naphthalene acetic acid) gel |
| CN105052928A (en) * | 2015-08-25 | 2015-11-18 | 钱夕华 | Preparing method for high-concentration NAA gel |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160608 |