CN105052911A - Preparation method of low-concentration IBA gel - Google Patents
Preparation method of low-concentration IBA gel Download PDFInfo
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- CN105052911A CN105052911A CN201510525492.0A CN201510525492A CN105052911A CN 105052911 A CN105052911 A CN 105052911A CN 201510525492 A CN201510525492 A CN 201510525492A CN 105052911 A CN105052911 A CN 105052911A
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- hormone
- iba
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Abstract
The invention discloses a preparation method of low-concentration IBA gel, and belongs to the field of plant biological technologies. First, IBA mother liquor of 1 mg/mL is prepared; the mother liquor is diluted to be low-concentration IBA working solution of 10 mg/L; the working solution is used for preparing the low-concentration IBA gel confining liquid of sodium carboxymethyl cellulose and sodium alginate, wherein the mass concentration of the sodium carboxymethyl cellulose is 1%, and the mass concentration of the sodium alginate is 8%.
Description
Technical field
A compound method for low concentration IBA gel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IBA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A compound method for low concentration IBA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A compound method for low concentration IBA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
Claims (1)
1. a compound method for low concentration IBA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
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CN201510525492.0A CN105052911A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration IBA gel |
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CN201510525492.0A CN105052911A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration IBA gel |
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CN105052911A true CN105052911A (en) | 2015-11-18 |
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CN201510525492.0A Pending CN105052911A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration IBA gel |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
CN103918650A (en) * | 2014-04-09 | 2014-07-16 | 河北省农林科学院昌黎果树研究所 | Growth regulator for improving fruit setting rate of peaches and regulating method |
-
2015
- 2015-08-25 CN CN201510525492.0A patent/CN105052911A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
CN103918650A (en) * | 2014-04-09 | 2014-07-16 | 河北省农林科学院昌黎果树研究所 | Growth regulator for improving fruit setting rate of peaches and regulating method |
Non-Patent Citations (1)
Title |
---|
张溃珍 等: "高分子·激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
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Application publication date: 20151118 |