CN105052937A - Preparation method for low-concentration IBA and glycerin gel - Google Patents
Preparation method for low-concentration IBA and glycerin gel Download PDFInfo
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- CN105052937A CN105052937A CN201510525393.2A CN201510525393A CN105052937A CN 105052937 A CN105052937 A CN 105052937A CN 201510525393 A CN201510525393 A CN 201510525393A CN 105052937 A CN105052937 A CN 105052937A
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- hormone
- iba
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Abstract
The invention discloses a preparation method for low-concentration IBA and glycerin gel, and belongs to the field of plant biological technologies. First, IBA mother liquor of 1 mg/mL is prepared; the mother liquor is diluted to be low-concentration IBA and glycerin working solution of 10 mg/L; the working solution is used for preparing the low-concentration IBA and glycerin gel confining liquid of sodium carboxymethyl cellulose and sodium alginate, wherein the mass concentration of the sodium carboxymethyl cellulose is 1%, and the mass concentration of the sodium alginate is 8%.
Description
Technical field
A compound method for low concentration IBA and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IBA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A compound method for low concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously according to the glycerine adding 10ml in the hormone working solution of 90ml;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A compound method for low concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously according to the glycerine adding 10ml in the hormone working solution of 90ml;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.
Claims (1)
1. a compound method for low concentration IBA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously according to the glycerine adding 10ml in the hormone working solution of 90ml;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition.
Priority Applications (1)
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CN201510525393.2A CN105052937A (en) | 2015-08-25 | 2015-08-25 | Preparation method for low-concentration IBA and glycerin gel |
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CN201510525393.2A CN105052937A (en) | 2015-08-25 | 2015-08-25 | Preparation method for low-concentration IBA and glycerin gel |
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CN201510525393.2A Pending CN105052937A (en) | 2015-08-25 | 2015-08-25 | Preparation method for low-concentration IBA and glycerin gel |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4942158A (en) * | 1988-10-13 | 1990-07-17 | Eastman Kodak | Transdermal steroid penetrant compositions and methods utilizing isopropanol and isobutanol |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525393.2A patent/CN105052937A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4942158A (en) * | 1988-10-13 | 1990-07-17 | Eastman Kodak | Transdermal steroid penetrant compositions and methods utilizing isopropanol and isobutanol |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
Non-Patent Citations (3)
Title |
---|
KAZIMIERA HENRYKA BODEK: "MICROCRYSTALLINE CHITOSAN AS PHARMACEUTICAL PREPARATION", 《POLISH CHITIN SOCIETY, MONOGRAPH XI》 * |
张溃珍 等: "高分子·激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
林鹏英: "甲基纤维素-海藻酸钠复合膜的制备及其性能研究", 《长春理工大学学报(自然科学版)》 * |
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Application publication date: 20151118 |
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