CN105052932A - Preparation method of intermediate-concentration 2,4-D gel - Google Patents
Preparation method of intermediate-concentration 2,4-D gel Download PDFInfo
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- CN105052932A CN105052932A CN201510525688.XA CN201510525688A CN105052932A CN 105052932 A CN105052932 A CN 105052932A CN 201510525688 A CN201510525688 A CN 201510525688A CN 105052932 A CN105052932 A CN 105052932A
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Abstract
The invention discloses a preparation method of intermediate-concentration 2,4-D gel and belongs to the technical field of plant biology. Firstly, 2,4-D mother liquor of 1 mg/mL is prepared; the mother liquor is diluted into an intermediate-concentration 2-4-D working solution of 100 mg/L; intermediate-concentration 2,4-D gel confining liquid with sodium carboxymethylcellulose with the mass concentration of 1% and sodium alga acid with the mass concentration of 8% is prepared through the working solution.
Description
Technical field
In one, the compound method of concentration 2,4-D gel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as 2,4-D, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
In one, the compound method of concentration 2,4-D gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
In one, the compound method of concentration 2,4-D gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
Claims (1)
1. the compound method of concentration 2,4-D gel in a kind, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition.
Priority Applications (1)
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CN201510525688.XA CN105052932A (en) | 2015-08-25 | 2015-08-25 | Preparation method of intermediate-concentration 2,4-D gel |
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CN201510525688.XA CN105052932A (en) | 2015-08-25 | 2015-08-25 | Preparation method of intermediate-concentration 2,4-D gel |
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CN105052932A true CN105052932A (en) | 2015-11-18 |
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CN201510525688.XA Pending CN105052932A (en) | 2015-08-25 | 2015-08-25 | Preparation method of intermediate-concentration 2,4-D gel |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525688.XA patent/CN105052932A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
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Application publication date: 20151118 |