CN105104372A - Preparation method of low-concentration 2,4-D gel - Google Patents
Preparation method of low-concentration 2,4-D gel Download PDFInfo
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- CN105104372A CN105104372A CN201510525741.6A CN201510525741A CN105104372A CN 105104372 A CN105104372 A CN 105104372A CN 201510525741 A CN201510525741 A CN 201510525741A CN 105104372 A CN105104372 A CN 105104372A
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Abstract
The invention discloses a preparation method of low-concentration 2,4-D gel, belonging to the field of plant biotechnology. The preparation method comprises the following steps: firstly, preparing 1mg/mL 2,4-D mother liquid; then, diluting the mother liquid into 10mg/L low-concentration 2,4-D working liquid; and making the working liquid into low-concentration 2,4-D gel sealing liquid with sodium carboxymethylcellulose with mass concentration being 1% and sodium alginate with mass concentration being 8%.
Description
Technical field
A kind of compound method of low concentration 2,4-D gel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as 2,4-D, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A kind of compound method of low concentration 2,4-D gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A kind of compound method of low concentration 2,4-D gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
Claims (1)
1. the compound method of low concentration 2, a 4-D gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition.
Priority Applications (1)
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CN201510525741.6A CN105104372A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration 2,4-D gel |
Applications Claiming Priority (1)
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CN201510525741.6A CN105104372A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration 2,4-D gel |
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CN105104372A true CN105104372A (en) | 2015-12-02 |
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CN201510525741.6A Pending CN105104372A (en) | 2015-08-25 | 2015-08-25 | Preparation method of low-concentration 2,4-D gel |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101501163A (en) * | 2006-06-12 | 2009-08-05 | 约瑟夫·帕特诺斯特 | Moisturizing agent with nutrients |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
-
2015
- 2015-08-25 CN CN201510525741.6A patent/CN105104372A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101501163A (en) * | 2006-06-12 | 2009-08-05 | 约瑟夫·帕特诺斯特 | Moisturizing agent with nutrients |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
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Application publication date: 20151202 |