CN105628679B - Infrared Raman UV fluorescence supercontinuum blood Discriminator - Google Patents

Infrared Raman UV fluorescence supercontinuum blood Discriminator Download PDF

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CN105628679B
CN105628679B CN201610167774.2A CN201610167774A CN105628679B CN 105628679 B CN105628679 B CN 105628679B CN 201610167774 A CN201610167774 A CN 201610167774A CN 105628679 B CN105628679 B CN 105628679B
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laser
fiber
infrared
spectral
optical
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CN105628679A (en
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万雄
刘鹏希
章婷婷
陈学岗
张志敏
张华明
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中国科学院上海技术物理研究所
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Abstract

本发明公开了种基于红外拉曼紫外荧光超连续谱的血液鉴别仪。 The present invention discloses an identification device types of blood-based Raman infrared supercontinuum UV fluorescence. 鉴别仪包括电源模组、可见及红外谱段光谱仪、两进两出光纤、主控及数据分析系统、积分球、试管机械手、连接杆、导轨、机械手运动控制器、样品室外盖板、底板、支架、光源室外盖板、光纤准直接头、激光器尾纤、超连续谱激光器、物镜支架、显微物镜、物镜联接器、光纤准直镜、Y形同轴光纤、第及第二光纤联接器、Y形光纤、红外及紫外窄线宽激光器。 Identification device includes a power supply module, the visible and infrared spectral spectroscopy, two-two optical fibers, the main control and data analysis systems, integrating sphere, manipulator tube, connecting rods, rails, the robot motion controller, sample chamber cover plate, a bottom plate, holder, a light source outside the cover, direct fiber collimator head, a laser pigtail, supercontinuum laser, an objective lens holder, a microscope objective, a coupling lens, a fiber collimator mirror, Y-shaped fiber coaxial, the first and the second optical coupler , Y-shaped fiber optics, infrared and ultraviolet narrow linewidth laser. 硬件采用Y形光纤联接两进两出光纤复用光谱仪,软件上采用分段加权叠加,实现红外拉曼光谱、紫外荧光光谱及超连续漫综合光谱数据信息融合。 Hardware using two coupled Y-shaped optical fiber into two multiplexed optical spectrometer, using a weighted stack segment software, infrared Raman spectroscopy, UV fluorescence spectroscopy and diffuse supercontinuum comprehensive spectral data fusion. 本发明的血液鉴别仪可适用于全血、血浆及血清的自动识别。 Blood identification device according to the present invention is applicable to whole blood, plasma and serum automatic identification.

Description

基于红外拉曼紫外荧光超连续谱的血液鉴别仪 Infrared Raman UV fluorescence supercontinuum blood Discriminator

技术领域 FIELD

[0001]本发明涉及一种人与动物血液分类伩器及方法,尤其涉及一种基于红外拉曼、紫外荧光结合超连续漫综合激光光谱的用于密封试管血液样品非接触式鉴别的仪器及方法。 [0001] The present invention relates to a classification of human and animal blood Xin and method, particularly to an infrared-based Raman, UV fluorescence binding supercontinuum laser spectroscopy instrument integrated diffuse sealed tube for blood samples and non-contact identification of method.

背景技术 Background technique

[0002]目前我国对于血液和其他各类生物材料的海关进出口均采取信用制度,而对各类生物材料的真实性却由于种种原因无法直接检测。 [0002] At present, China Customs import and export for blood and other biological materials of all kinds are taking credit system, and the authenticity of the various types of biological material, but for various reasons can not be directly detected. 特别是涉及血液样品这样的特殊材料, 开放式的接触式采样很多时候条件是不被允许的。 More particularly, to a blood sample special materials, open-contact sampling conditions often are not allowed. 一方面血液样品可能被检测操作污染; 另一方面血液样品自身可能携带的致病因子会对检测人员造成职业暴露。 Aspect of the blood sample may be detected operation pollution; on the other hand the blood sample itself may carry the causative agent will cause occupational exposure testing personnel. 鉴于上述原因, 开发血液样品非接触式快速检测技术方法十分急迫。 For these reasons, the development of a blood sample non-contact method for the rapid detection of very urgent.

[0003]人类血液与动物全血主成分相似,主要由血细胞和血浆组成,都呈红色,肉眼难以区别,但人与动物血细胞和血浆的形貌和组成是有细微区别的,选用恰当的方法就可以区分人类血液与动物血液。 [0003] Similar human blood and animal whole blood main component, is mainly composed of blood cells and plasma composition, radiate red, visually indistinguishable, but human and animal blood cells and plasma of the morphology and composition are slightly different, by appropriate means We can distinguish human blood and animal blood. 经典的接触式的检测方法可以检测出部分血液参数,可以根据这些血液参数进行不同种属之间的血液鉴别。 The classic method of detecting contact portion can be detected in blood parameters, blood discrimination between different species of blood according to these parameters. 目前,商用使用较多的血液鉴别产品大都基于流式细胞术,需进行接触式微采样得到血液的代表种属特性的参数。 Currently, more commercial use of blood products are mostly based on flow cytometry to identify, in need of contact micro sampling parameters to be represented species characteristic of the blood. 非接触式密封血液鉴别是一个极富挑战的课题,因为大部分物种密封在透明试管中的无论是全血还是血清、血浆等样品,其在紫外、可见、红外谱段,对外所反映的光学特性极其相似,在非采样的检测中,依靠光学的手段分辨极其困难。 Identify the non-contact seal blood is a challenging problem, since the sealing of the transparent tube or whether it is whole blood serum and plasma samples most species, which in the ultraviolet, visible, infrared spectral, as reflected in the external optical very similar properties, the non-detection of sample, rely on the optical means is extremely difficult to distinguish.

[0004]首先,试管内封装的血液制品可能含有不同成份的抗凝剂,包括肝素、乙二胺四乙酸盐(EDTA盐)、枸橼酸盐、草酸盐等,此外试管材料可能是石英玻璃或PET塑料等,大部分试管还都贴有标签。 [0004] First, in vitro packaging of blood products may contain different ingredients anticoagulants, including heparin, ethylenediaminetetraacetic acid (EDTA salts), citrate, oxalate and the like, in addition to the tube material may be quartz glass or PET plastic, etc., most of the tubes are also labeled. 这些干扰因素,将严重影响血液的光学性质,使得常用光学及光谱方法在密封试管血液鉴别上无能为力。 These disturbances, will seriously affect the optical properties of blood, so that the optical and spectroscopic methods commonly used in the inability of blood sealed tube identification.

[0005]鉴于以上原因,开发一种用于人与动物密封试管血液,包括全血、血清、血浆的非接触式分类鉴别仪器及相应方法是急需解决的难题。 [0005] For these reasons, development of a sealed tube for human and animal blood, including whole blood, serum, plasma Discrimination of non-contact apparatus and corresponding method are urgently needed to solve the problem.

[0006]针对该问题,本发明提出一种基于红外拉曼、紫外荧光结合可见至中红外超连续漫综合激光光谱的用于密封试管血液样品非接触式鉴别的仪器及方法,在红外拉曼及紫外^光光谱检测中采用窄线宽激光器,结合光纤准直及显微物镜聚焦至试管内血液样品,并采用发射接收同轴光纤端面设计采集血液样品后向拉曼及荧光散射信号;在超连续漫综合激光光谱检测中,采用宽光谱超连续谱激光源及特殊设计的积分球为核心仪器硬件结构, 得到不同样品的漫综合激光光谱数据。 [0006] To solve this problem, the present invention provides a method and apparatus to be seen in conjunction with a sealed tube a blood sample of a non-contact identification integrated diffuse infrared supercontinuum laser Raman spectroscopy based on infrared, UV fluorescence, infrared Raman ^ spectroscopy and UV light in a narrow linewidth laser, in conjunction with the fiber collimator and focusing the microscope objective into the blood sample tube, and using the signal to Raman scattering and fluorescence emission after receiving the fiber end faces coaxially design blood samples were collected; the supercontinuum laser spectroscopy detection integrated diffuse, broad spectrum integrating sphere supercontinuum laser light source and a special design of the hardware structure of the core instruments, to obtain an integrated laser diffuse spectral data of different samples. 本发明的方法结合以上三种激光光谱检测技术,并采用Y形光纤联接两进两出光纤复用光谱伩,实现光谱数据信息融合。 The method of the present invention in conjunction with the above three kinds of laser spectroscopy techniques, using two coupled Y-shaped optical fiber into two optical multiplexing Xin spectrum, the spectral data to achieve fusion. 建立不同物种、不同试官、不冋血液的rill合光谱数据库,并基于主成分分析法(principal components analysis,简称PCA)计算这些融合光谱数据得到主成分分析得分图,在得分图中得到人与动物全血、血浆、血清的聚类区域,将这些区域作为鉴别判断标准进行人与动物试管密封血样非接触鉴别。 Establishing different species, different interviewer, not Jiong blood rill spectrometry database, based on principal component analysis (principal components analysis, referred to as PCA) to calculate these fusion spectral data to obtain the principal components analysis FIG obtain human and scoring figures animal whole blood, plasma, serum clustering regions, these regions of non-contact identification of human and animal blood sample tube was sealed as the authentication criteria.

发明内容 SUMMARY

[0007]本发明的目的在于提供一种基于红外拉曼、紫外焚光结合可见至中红外超连续漫综合激光光谱的用于密封试管血液样品非接触式鉴别的仪器,可进行人与动物全血、血浆及血清的自动识别,满足检测检疫部门对血液制品粗检的需求。 [0007] The object of the present invention is to provide an infrared-based Raman, ultraviolet light burning binding visible to infrared diffuse integrated supercontinuum laser spectrum for a blood sample tube sealed non-contact identification equipment, people and animals can be whole blood, plasma and serum of automatic identification, detection needs crude quarantine inspection department of blood products.

[0008]本发明的技术方案是这样来实现的,基于红外拉曼、紫外荧光结合超连续漫综合激光光谱的密封血液鉴别仪的硬件系统主要由电源模组、可见谱段光谱仪、红外谱段光谱仪、两进两出光纤、主控及数据分析系统、积分球、试管机械手、连接杆、导轨、机械手运动控制器、样品室外盖板、底板、支架、光源室外盖板、光纤准直接头、激光器尾纤、超连续谱激光器、物镜支架、显微物镜、物镜联接器、光纤准直镜、Y形同轴光纤、第一光纤联接器、第二光纤联接器、Y形光纤、红外窄线宽激光器及紫外窄线宽激光器组成。 [0008] The aspect of the present invention is achieved, based on infrared Raman, UV fluorescence binding supercontinuum laser spectroscopy diffuse integrated sealing blood analyzer hardware identification system consists of the power module, the visible spectral spectrum, infrared spectral spectroscopy, two-two optical fibers, the main control and data analysis systems, integrating sphere, manipulator tube, connecting rods, rails, the robot motion controller, sample chamber cover plate, base plate, the bracket, the light source outside the cover, direct fiber collimator head, laser pigtail, supercontinuum laser, an objective lens holder, a microscope objective, a coupling lens, a fiber collimator mirror, Y-shaped optical coaxially, a first fiber coupler, second fiber coupler, Y-shaped optical fiber, infrared narrow line width of narrow linewidth laser and ultraviolet lasers.

[0009]其中,积分球由积分球右半部和积分球左半部两个半球组成,它们通过积分球接头进行联接,并安装固定在底板上。 [0009] wherein the integrating sphere integrating sphere integrating sphere left and right portions of the two hemispheres, they are coupled by fitting integrating sphere and fixed to the base plate. 积分球内壁涂覆漫反射涂层,对照射到内壁的光线起漫反射匀光的作用。 The inner wall of the integrating sphere coated with diffusely reflective coating, the light radiated onto the inner wall acts uniform diffuse reflection of light. 积分球右半部开有积分球样品孔,样品室外盖板安装在底板及积分球右半部上,形成封闭空间样品室,以消除杂散光的影响。 The right half of the integrating sphere integrating sphere sample wells are opened, sample chamber cover mounted on the base portion and the right half of the integrating sphere, the sample chamber forms a closed space, in order to eliminate the influence of stray light. 样品室外盖板上方开有圆孔,装卸主轴穿过积分球样品孔及圆孔的中心,并与底板垂直。 Sample chamber above the circular opening cover, detachably spindle through the center of the integrating sphere and circular holes of the sample and perpendicular to the base plate. 积分球左半部开有积分球光源孔及积分球光纤接口。 Integrating sphere left portion is opened and holes integrating sphere integrating sphere optical interface.

[0010] Y形同轴光纤由激光发射光纤与信号接收光纤组成,两者汇聚成一根光纤,其光纤端面为同轴分布,中心圆形区域为激光发射光纤排列,外圈同心圆环区域为信号接收光纤排列,该几何配置可高效接收红外激光激发的后向拉曼散射及紫外激光激发的后向荧光信号。 [0010] Y-shape coaxial with the optical signal emitted by the laser fiber receiving optical fibers, both the converging an optical fiber distribution of the fiber end faces are coaxial, arranged in the central circular zone emitting a laser fiber, the outer concentric annular region signal receiving fiber arrangement, the geometrical configuration of the excitation to the UV laser Raman scattering and efficiently to receive infrared laser excitation fluorescent signal. Y形同轴光纤与光纤准直镜耦合可实现准直发射与接收,光纤准直镜通过物镜联接器与显微物镜相联接,可将红外及紫外激光束聚焦至光谱测试点并对光谱测试点的后向散射信号进行收集。 Y-shaped optical fiber and coaxial coupling may realize a collimator lens collimating the transmitting and receiving, by the optical fiber collimator lens and a coupling lens coupled to a microscope objective, infrared and ultraviolet laser beam may be focused to a point and spectra spectroscopy after the point of collection to the scattering signal. 物镜支架将显微物镜固定安装于底板上。 The microscope objective lens holder is fixedly mounted on the bottom plate.

[0011] Y形光纤有两个输入端及一个输出端,两个输入端分别联接红外窄线宽激光器与紫外窄线宽激光器,输出端通过第二光纤联接器与Y形同轴光纤中的激光发射光纤相联接。 [0011] Y-shaped fiber has two input terminals and an output terminal, two input terminals respectively coupled to the infrared narrow linewidth laser of UV laser narrow linewidth output terminal through the second optical fiber with a Y-shaped coupler optical fiber coaxially in the optical fiber coupled to the laser emission. Y形光纤可将红外窄线宽激光器发出的红外激光与紫外窄线宽激光器发出的紫外激光同时汇集到激光发射光纤中。 UV ultraviolet laser and infrared laser light emitted by the laser narrow linewidth Y shaped fiber can be emitted from an infrared laser, narrow linewidth laser emission simultaneously together to the optical fiber.

[0012]可见谱段光谱仪与红外谱段光谱仪均采用相同光谱伩光纤接口,两进两出光纤分为两个接收端(即两进)及两个输出端(即两出)。 [0012] Visible spectroscopy and infrared spectral spectral spectroscopy spectrum Xin use the same optical interface, into two optical fibers into two two receiving ends (i.e., two feed) and two outputs (i.e., two). 一个接收端与积分球光纤接口联接,可收集来自接收光轴的漫反射光线;另一个接收端与信号拉曼接收光纤通过第一光纤联接器联接,可收集来自信号接收光纤的拉曼及荧光信号;两个输出端分别与可见谱段光谱仪及红外谱段光谱仪联接。 A receiving end and coupled to the integrating sphere optical fiber interface may be collected from the diffuse light receiving axis; the other end of the signal received by the first receiving fiber Raman optical fiber coupler coupling may be collected and the fluorescence from the Raman signal receiving fiber signal; two spectral output of the visible spectrometer and an infrared spectrometer coupled spectral respectively. < <

[0013]超连续谱激光器发出的可见及红外谱段超连续谱脉冲激光经激光器尾纤传输,然后通过光纤准直接头进行光束准直后输出超连续谱脉冲准直激光束,并沿发射光轴穿过积分球光源孔进入积分球。 [0013] supercontinuum laser, visible and infrared spectral range emitted by the pulsed laser supercontinuum laser pigtail transmission, then the output pulses of the supercontinuum light beam collimated by the collimated laser beam direct fiber collimator head, and emit light along shaft through hole into the integrating sphere integrating sphere. 7 " 7 "

[0014]光纤准直接头由支架固定在底板上,并通过光源室外盖板与积分球左半部外壳联接,形成封闭空间光源室,以消除杂散光的影响。 [0014] Effect of Fiber Alignment head by a bracket fixed on the chassis, and the left half portion of the housing and coupled to the integrating sphere, a closed space is formed by a light source outside the chamber cover plate, to eliminate stray light.

[0015]红外窄线宽激光器发出的红外连续激光束经Y形光纤至Y形同轴光纤中的激光发射光纤传输、从激光发射光纤排列中发射后沿激光光轴传输、经光纤准直镜准直、显微物镜聚焦后可实现对光谱测试点的红外诱导拉曼激发(注:激光光轴与装卸主轴相交,其交点为光谱测试点),光谱测试点的后向散射拉曼信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至红外谱段光谱仪进行接收和分析。 [0015] continuous infrared laser beam emitted from the infrared laser narrow linewidth Y shaped fiber through the Y-shaped coaxial to the laser emission in the optical fiber transmission, the laser light emitted from the emitting fiber aligned along the optical axis of laser light transmitted through the optical fiber collimator lens collimation, infrared spectroscopy can be achieved points after induction of Raman excitation microscope objective focusing (Note: the laser optical axis intersects the spindle and handling, which is the intersection point spectroscopy), after the spectra backscattered Raman signal points sequentially after the microscope objective lens and a fiber collimator, an optical fiber signal receiver that is arranged in the outer concentric annular region is collected by the Y-shaped fiber end face coaxially, and then via signal receiving fiber, into two optical fibers to two infrared spectral spectrometer received and analysis.

[0016]紫外窄线宽激光器发出的紫外连续激光束经Y形光纤至Y形同轴光纤中的激光发射光纤传输、从激光发射光纤排列中发射后沿激光光轴传输、经光纤准直镜准直、显微物镜聚焦后可实现对光谱测试点的紫外诱导荧光激发,光谱测试点的后向散射荧光信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至可见谱段光谱仪进行接收和分析。 [0016] UV ultraviolet laser beam is continuously emitted by the laser narrow linewidth Y Y-shaped fiber coaxial to the laser emission in the optical transmission fiber, the laser light emitted from the emitting fiber aligned along the optical axis of laser light transmitted through the optical fiber collimator lens a collimator, focusing the microscope objective can be achieved by UV spectroscopy induced fluorescence excitation points, test points after the spectrum scattered fluorescence signals sequentially to the outer ring via a microscope objective lens and the optical fiber by the collimator coaxially Y-shaped fiber endface concentric annular region, i.e., an optical fiber signal receiver arranged to collect, and then received by the optical signal into two optical fibers to two visible spectrometer for spectral analysis and reception.

[0017]导轨与底板垂直安装,试管机械手通过连接杆与导轨联接并可在机械手运动控制器控制下沿导轨滑动。 [0017] The vertical mounting rail and the base plate, a test tube and the manipulator is coupled through a connecting rod with the rail slides along the guide rail in the robot motion controller. 待检血液由试管盖封装在试管中。 Blood sample to be encapsulated by a cap tubes in a test tube. 试管机械手在机械手运动控制器控制下,可抓紧试管盖并带动试管沿装卸主轴方向上下运动。 Tubes at the robot manipulator motion controller, can grasp the tube and drive tube cap in the direction of loading of the spindle up and down.

[0018]电源模组用以对可见谱段光谱仪、红外谱段光谱仪、主控及数据分析系统、超连续谱激光器、红外窄线宽激光器、紫外窄线宽激光器及机械手运动控制器进行供电。 [0018] The power module is used for the visible spectral spectrum, infrared spectral spectrometer, and data analysis system master, supercontinuum laser, infrared narrow linewidth lasers, ultraviolet narrow linewidth laser and the robot motion control power supply. 主控及数据分析系统用以对可见谱段光谱仪、红外谱段光谱仪、超连续谱激光器、红外窄线宽激光器、紫外窄线宽激光器及机械手运动控制器进行控制,并通过USB接口接收可见谱段光谱仪及红外谱段光谱仪输出的光谱数据进行处理和分析。 Master and data analysis system for the visible spectral spectrum, infrared spectroscopy spectral supercontinuum laser, infrared narrow linewidth lasers, ultraviolet narrow linewidth laser and the robot controller controls the motion, and receiving the visible spectrum through the USB interface spectral data segment and infrared spectral spectrometer spectrometer output for processing and analysis. 主控及数据分析系统内含触摸屏人机交互界面,用于与用户的人机交互,接受用户的指令并输出结果给用户。 And master data analysis system contains interactive touch screen interface for human-computer interaction with the user, receiving a user's instruction and outputs the result to the user.

[0019]基于红外拉曼、紫外荧光结合超连续漫综合激光光谱的密封血液鉴别仪的血液分析方法其步骤为: A blood analysis method of sealing a blood Discriminator [0019] Infrared Raman, UV fluorescence binding supercontinuum integrated diffuse the laser spectrum comprises the following steps:

[0020] ⑴仪器启动及超连续谱测试进样 [0020] ⑴ instrument start-up and test sample supercontinuum

[0021]开启电源模组,对可见谱段光谱仪、红外谱段光谱仪、主控及数据分析系统、超连续谱激光器、红外窄线宽激光器、紫外窄线宽激光器及机械手运动控制器进行供电。 [0021] Turn on the power module, the visible spectral spectrum, infrared spectral spectrometer, and data analysis system master, supercontinuum laser, infrared narrow linewidth lasers, ultraviolet narrow linewidth laser and the robot motion control power supply.

[0022]用户通过触摸屏人机交互界面启动测试主程序。 [0022] user-initiated test main program through the touch screen man-machine interface. 此时,主控及数据分析系统发出进样指令给机械手运动控制器,机械手运动控制器控制试管机械手抓取试管进样至其沿装卸主轴方向进入积分球。 At this time, the main control and data analysis system issues a command to the injector controller robot motion, motion controller controls the robot manipulator gripping the tube to the injection tube which enters the integrating sphere in the main loading direction. 试管底部位置高于发射光轴且与接收光轴不相交,避免激光束直接照射待检试管,并避免试管的透反射光线直接沿发射光轴传输并被两进两出光纤收集。 Position higher than the bottom of the tube and does not intersect the axis of a transmitting light receiving axis, through avoiding direct exposure to the laser beam reflected ray tube to be tested, the test tube and to avoid direct emission along the optical axis and two transmission fibers collected into two. [0023] ⑵超连续谱激光漫综合光谱测试 [0023] ⑵ diffuse integrated supercontinuum laser spectroscopy

[0024]主控及数据分析系统发出指令启动超连续谱激光器、可见谱段光谱仪及红外谱段光谱仪。 [0024] Master and the data analysis system issues a command to start supercontinuum laser, visible and infrared spectral spectrometer spectral spectrometer. 超连续谱激光器输出的超连续谱准直激光束沿发射光轴进入积分球,激光束照射3漫反射涂层后,其反射光为漫反射,即在积分球内沿各方向传输,变成了均匀光线。 Supercontinuum laser output supercontinuum along emitting collimated laser beam into the optical axis of the integrating sphere, the laser beam 3 diffusely reflecting coating which reflects light diffuse reflection, i.e. in each direction in the transport sphere, into uniform light. 试管受不同方向的超连续谱光线照射后,试管(注:包括其材质与外贴标签)与待检血液的漫反射、漫透射、吸收、发射后的光线将沿空间任意方向传输,遇到漫反射涂层漫反射至任意方向,其对积分球内的光线传输具有漫综合光谱影响。 After the tube supercontinuum light irradiation of different directions, the tube (Note: including its material and an outer labeling) and diffusely reflected to be detected blood, diffuse transmission, absorption of light after transmitting the transmission in any direction of space encountered diffusely diffuse reflective coating to an arbitrary direction, and the light transmission of a diffuse integrating sphere having an integrated impact spectra.

[0025]沿发射光轴传输的漫反射光线被两进两出光纤收集后,分别送至可见谱段光谱仪及红外谱段光谱仪进行光电转换变成光谱数据。 After the [0025] two optical fibers are collected into two along the optical axis emitted diffuse light transmission are supplied respectively to the visible and infrared spectral spectrometer spectral spectrometer photoelectric conversion into spectral data. 可见谱段光谱仪的采样点为N1,采样点数取Nl = 1300,红外谱段光谱仪的采样点为N2,采样点数取N2 = 512;可见谱段光谱仪及红外谱段光谱仪输出的共N=N1+N2个光谱数据经USB接口送至主控及数据分析系统进行存储。 Visible samples spectral spectrometer as N1, the number of samples taken Nl = 1300, sampling points infrared spectral spectrometer as N2, sampling points taken N2 = 512; total N = N1 visible spectral spectroscopy and infrared spectral spectrometer output + N2 spectral data is stored to the host via the USB interface and data analysis system. [0026] ⑶拉曼荧光光谱测试进样 [0026] ⑶ Raman spectroscopy sample fluorescence

[0027]主控及数据分析系统发送出样指令给机械手运动控制器,机械手运动控制器控制试管机械手带动试管至其沿装卸主轴方向移出积分球及样品室,至光谱测试点位于试管内待检血液的中心位置,此时完成拉曼荧光光谱测试进样。 [0027] The master control and data analysis system for transmitting a kind of instruction to the robot motion controller, the robot motion controller tubes robot drive tube to move it out of the integrating sphere and a sample chamber in handling major axis direction, to the spectral test point is to be examined in vitro the center position of the blood, fluorescence spectroscopy Raman This completes the injection. ...

[0028] 红外激光拉曼光谱及紫外激光荧光测试 [0028] Infrared Raman spectroscopy and UV laser fluorescence test

[0029]主控及数据分析系统发出指令启动红外窄线宽激光器、紫外窄线宽激光器、可见谱段光谱仪及红外谱段光谱仪。 [0029] Master and the data analysis system issues a command to start an infrared narrow linewidth lasers, narrow linewidth laser UV, visible spectroscopy and infrared spectral spectral spectrometer.

[0030]紫外窄线宽激光器发出的紫外连续激光束经准直聚焦后会聚到光谱测试点处的待检血液,紫外窄线宽连续激光所诱导的荧光光谱信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至可见谱段光谱仪进行光电转换变成光谱数据,对荧光光谱采用与超连续谱测试可见谱段一样的采样位置与采样点数,g卩N3 = N1。 Continuous UV laser beam [0030] UV narrow linewidth laser emitted by the collimator focusing to be examined converge blood, narrow linewidth UV spectroscopy at continuous laser induced fluorescence spectrum point signal sequentially through a microscope objective and fiber collimators after the Y-shaped collimator lens coaxial concentric outer region of the fiber end face of the ring, i.e., signal receiving fiber arrangement collected, and then the received optical signal, two optical fibers into two spectral bands visible spectrometer to photoelectrically converted into spectral data, fluorescence spectra were obtained using a supercontinuum test with the visible spectral sampling positions as sampling points, g Jie N3 = N1. 可见谱段光谱仪输出的⑽个光谱数据经USB接口6送至主控及数据分析系统7进行存储。 ⑽ the visible spectral data of the spectrometer spectral output to the host via the USB interface 6 and 7 store the data analysis system.

[0031]同时,红外窄线宽激光器发出的红外连续激光束经准直聚焦后会聚到光谱测试点处的待检血液,红外窄线宽连续激光所诱导的斯托克斯拉曼光谱信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至红外谱段光谱仪进行光电转换变成光谱数据,对拉曼光谱采用与超连续谱测试红外谱段一样的采样位置与采样点数,g卩N4 = N2。 [0031] Meanwhile, the infrared laser beam continuously emitted from the infrared narrow linewidth laser focused through converging collimator blood to be examined at the test point spectrum, infrared narrow linewidth continuous laser induced Stokes Raman spectroscopy signals sequentially , i.e. the signal received after the microscope objective lens and an optical fiber collimator of the outer coaxial concentric annular-shaped regions Y arranged fiber end collecting fiber, and then received by the optical signal into two optical fibers to two infrared spectral photoelectric spectrometer converts into spectral data, and Raman spectra were obtained using a supercontinuum test infrared spectral sampling positions as sampling points, g Jie N4 = N2. 红外谱段光谱仪输出的N4个光谱数据经USB接口送至主控及数据分析系统进行存储。 Infrared spectral data N4 spectral spectrometer outputted via the USB interface to the host memory and data analysis system.

[0032] (5)数据分析及融合 [0032] (5) Analysis and Data Fusion

[0033]将可见谱段的超连续谱激光漫综合光谱的N1个光谱数据与荧光光谱的N3个光谱数据进行加权叠加,得到N1个可见谱段的融合光谱数据。 [0033] The spectral data of the visible N3 supercontinuum laser integrated spectrum N1 diffuse spectral data of the fluorescence spectrum of the weighted spectral superposition to obtain spectral data of N1 fusion visible spectral ranges. 其中荧光光谱的权重值为F,超连续谱激光漫综合光谱的权重值为1-F。 Wherein the heavy weight of the fluorescence spectrum is F, right supercontinuum laser integrated diffuse spectral weight is 1-F. (注:本实施例中F=0.7) (Note: In Example F of the present embodiment = 0.7)

[0034] 将红外谱段的超连续谱激光漫综合光谱的N2个光谱数据与拉曼光谱的N4个光谱数据进行加权叠加,得到N2个红外谱段的融合光谱数据。 [0034] The supercontinuum laser diffuse N2 integrated spectrum data of infrared spectra and Raman spectra of spectral N4 of spectral data weighted superposition, the spectral data obtained fused N2 infrared spectral ranges. 其中拉曼光谱的权重值为L,超连续谱激光漫综合光谱的权重值为1-L。 Wherein the heavy weight of the Raman spectrum is L, the right supercontinuum laser integrated diffuse spectral weight is 1-L. (注:本实施例中L=0.4) (Note: this embodiment L = 0.4)

[0035] 将N1个可见谱段的融合光谱数据与N2个红外谱段的融合光谱数据组成共N个光谱数据用于后续分析。 [0035] The fused fusion spectrum visible spectral data of N1 and N2 spectral infrared spectral data consisting of a total of N spectral data for subsequent analysis. 基于主成分分析法(principal components analysis,简称PCA)计算这N个光谱数据的M个主成分数值,进行降维处理,主成分数M取为7。 Calculating the N spectral data based on principal component analysis (principal components analysis, referred to as the PCA) M principal component values, reduce the dimension, number of principal components is taken as M 7.

[0036] ⑹血液鉴别与判定 [0036] ⑹ Identification and determination of blood

[0037] 将待检血液的M个主成分数值,得到其在M维主成分空间的特征向量,将该特征向量与M维主成分空间的由本血液鉴别仪获得的人与动物全血、血浆、血清M维主成分空间数据库的聚类中心特征向量进行对比,根据特征向量相似度首先确定待检血液的血液类型, 即是全血、血浆还是血清。 [0037] the M principal component values ​​to be the subject of blood, resulting eigenvectors in the M-dimensional principal component space, and the person is obtained by the present blood identification device with the animal eigenvectors and M-dimensional principal component space of the whole blood, plasma , the cluster centers serum M-dimensional feature vector database principal component space are compared, the similarity feature vector to be tested is first determined according to the type of blood in the blood, i.e., whole blood, plasma or serum. 然后,再将待检血液的M个主成分数值,与该血液类型下的人与动物不同种属的M维主成分空间数据库的聚类中心特征向量进行对比,根据特征向量相似度再确定种属,即是人还是动物血液,如果是动物血液,是何种动物血液,至此,完成待检血液的血液类型与种属的判定。 Then, the value M and then principal components of the blood to be tested, the central cluster of feature vectors belong to different species in the human and animal blood type M-dimensional principal component space database comparison, and then determine the types of the feature similarity vector belongs, that is, human or animal blood, animal blood if it is, what kind of animal blood, so far, to be complete blood test blood types and species determination.

[0038]然后,主控及数据分析系统将待检血液的红外激光诱导拉曼光谱、紫外激光诱导荧光光谱及可见红外超连续漫综合激光光谱的光谱曲线以及判定结果显示在触摸屏人机交互界面上,以供用户参考。 Infrared laser [0038] Then, the master and the data analysis system of blood to be tested induced Raman spectroscopy, laser induced fluorescence spectroscopy and UV-visible spectrum curve integrated diffuse infrared supercontinuum laser spectrum and a determination of the touch screen display results in the man-machine interface on, for user reference. 至此完成整个测试过程。 This completes the entire testing process.

[0039]本发明的有益效果是,在红外拉曼光谱及紫外荧光光谱检测中采用窄线宽激光器,结合光纤准直及显微物镜聚焦至试管内血液样品,并采用发射接收同轴光纤端面设计采集血液样品后向拉曼及荧光散射信号,有效提高试管内血液激光红外拉曼及紫外突光光谱信号的信噪比;采用特殊设计的积分球,无论是血浆、血清透明液体的漫透射,还是全血及试管标签的漫反射,以及整个测试样品的漫吸射及漫发射的宽谱段信号均可接收,即可适用于各类血液、各类材质试管、各类不同抗凝剂以及不同标签情况下的光谱数据均匀采集。 [0039] Advantageous effects of the present invention is the use of Raman spectroscopy in the infrared and ultraviolet fluorescence spectroscopy narrow linewidth laser, in conjunction with the fiber collimator and focusing the microscope objective into the blood sample tube, and using the coaxial transmitting and receiving fiber ends after blood samples were collected designed to Raman scattering and fluorescence signals, improve the signal to noise ratio of blood projecting infrared laser Raman and UV light spectrum signal, in vitro; integrating sphere using a specially designed, either plasma, serum diffuse transmission transparent liquid , whole blood and diffuse reflection or tube labels, the entire test sample and the diffused jet suction broad spectral range and diffusely transmitted signal can be received, it can be applicable to all types of blood, various types of tube material, different types anticoagulants spectral data in the case of homogeneous and of different labels collection. 采用了可见至中红外高功率超连续谱激光源及对应的双谱段光谱仪,得到宽谱段漫综合激光光谱数据,可对封装血液的细微光学差异进行捕捉,可提高鉴别准确性;硬件采用Y 形光纤联接两进两出光纤复用光谱仪,软件上采用加权叠加,实现光谱数据信息融合。 Using visible to infrared high-power supercontinuum laser light source and the corresponding bis spectral spectrometer, to give broad spectral diffuse integrated laser spectroscopy data, can capture subtle optically different encapsulation blood can be improved authentication accuracy; hardware employed two Y-shaped fiber coupler into two optical multiplexed optical spectrometer using the weighted stack software, the spectral data fusion. 由于硬件结构和软件分析的鲁棒性,本发明的血液鉴别仪可适用于全血、血浆及血清的自动识别。 Since the hardware configuration and software robustness analysis, identification of the blood analyzer of the present invention is applicable to whole blood, plasma and serum automatic identification.

附图说明 BRIEF DESCRIPTION

[0040]图1为本发明的原理图,其中(a)超连续谱激光漫综合光谱测试;〇3)红外激光拉曼及紫外激光荧光光谱测试;(c)光纤剖面1图;(d)光纤剖面2图;(e)光纤剖面3图。 [0040] FIG. 1 is a schematic diagram of the present invention, wherein (a) a supercontinuum laser integrated diffuse spectra; 〇3) infrared laser Raman laser fluorescence and UV spectra; (c) fiber cross-section of FIG. 1; (d) fiber cross-sectional view of FIG. 2; (e) fiber cross-section 3 in FIG. 图中: 1--电源模组;2 可见谱段光谱仪;3--红外谱段光谱仪;4--光谱仪光纤接口; 5--两进两出光纤;6--USB接口; 7--主控及数据分析系统;8——积分球光纤接口; 9 积分球接头;10 试管机械手;11--连接杆;12--导轨;13--机械手运动控制器;14--试管盖;I5 试管;I6--圆孔;17--积分球样品孔;18——样品室外盖板; 19--待检血液;2〇接收光轴;21--样品室;22--底板;23--积分球右半部; 24 漫反射涂层;25 发射光轴;26--积分球左半部;27--光源室;28--积分球光源孔;29 支架;30 光源室外盖板;31--光纤准直接头;32--激光器尾纤; 33--触摸屏人机交互界面;34--超连续谱激光器;35——积分球;36——装卸主轴; 37 激光光轴;38 光谱测试点;39--物镜支架;40--显微物镜;41--物镜联接器;42--光纤准直镜;43--Y形同轴光纤;44——第一光纤联接器;45——激光发射光纤;妨--信号接收光纤;47--信号 FIG: 1-- power module; 2 visible spectral spectrometer; 3-- infrared spectral spectrometer; 4-- spectrometer fiber interface; 5-- two into two optical fibers; 6 - USB interfaces; main 7-- system control and data analysis; 8-- integrating sphere optical interface; integrating ball joint 9; 10 tube manipulator; 11-- connecting rod; 12-- rail; 13-- robot motion controller; 14-- test tube caps; I5 tube ; I6-- circular; 17-- integrating sphere sample wells; 18-- sample chamber cover; 19-- blood to be tested; 2〇 receiving axis; 21-- sample chamber; 22-- floor; 23-- the right half of the integrating sphere; diffusely reflective coating 24; 25 emitting an optical axis; 26-- integrating sphere left half portion; 27-- source chamber; 28-- integrating sphere aperture; bracket 29; a light source outside the cap plate 30; 31 - fiber Alignment head; 32-- laser pigtail; 33-- interactive touch-screen interface; 34-- supercontinuum laser; 35-- integrating sphere; 36-- detachably spindle; the laser optical axis 37; 38 spectrum test point; 39-- lens holder; 40-- microscope objective; 41-- coupling lens; 42-- fiber collimator; 43 - Y-shaped optical fiber coaxially; 44-- a first optical coupler; 45 - fiber laser emission; nuisance - signal receiving fiber; 47-- signal 收光纤排列;48——激光发射光纤排列;49——红外窄线宽激光器;5〇--第二光纤联接器;51——Y形光纤;52——紫外窄线宽激光器。 Receiving fiber arrangement; 48-- emitting laser fiber arrangement; 49-- infrared narrow linewidth laser; 5〇-- second fiber coupler; 51 - Y shaped fiber; 52-- ultraviolet narrow linewidth laser.

具体实施方式 Detailed ways

[0041]基于红^卜拉曼、紫外荧光结合超连续漫综合激光光谱的密封血液鉴别仪的硬件系统结构如图1所示,硬件系统主要由电源模组1、可见谱段光谱仪2、红外谱段光谱仪3、两进两出光纤5、主控及数据分析系统7、积分球3f5、试管机械手1〇、连接杆n、导轨12、机械手运动控制器13、样品室外盖板18、底板22、支架29、光源室外盖板30、光纤准直接头31、激光器尾纤32、超连续谱激光器34、物镜支架39、显微物镜40、物镜联接器41、光纤准直镜42、Y形同轴光纤43、第一光纤联接器44、第二光纤联接器50、Y形光纤5丨、红外窄线宽激光器49、紫外窄线宽激光器52组成。 [0041] ^ Bu Laman based on red, a combination of hardware architecture UV fluorescence seal blood identification device integrated diffuse supercontinuum laser spectrum as shown, mainly by the power module hardware system 1, the visible spectral spectrometer 12, the infrared spectral spectrometer 3, two optical fibers 5 into two, and the master data analysis system 7, an integrating sphere 3F5, 1〇 manipulator tube, connecting rod n, the guide rail 12, the robot motion controller 13, the cover 18 outside the sample chamber, the bottom plate 22 , bracket 29, the cover 30 outside the light source, direct fiber collimator head 31, a laser pigtail 32, supercontinuum laser 34, the lens holder 39, the microscope objective 40, the coupling lens 41, an optical fiber collimator 42, Y tantamount axis of the fiber 43, a first optical coupler 44, a second fiber optic connector 50, Y-shaped fiber 5 Shu, an infrared narrow linewidth lasers 49, 52 composed of narrow linewidth laser UV. _2]其中4只分球35由积分球右半部23和积分球左半部26两个半球组成,它们通过积分球接头9进行联接,并安装固定在底板22上。 2] wherein four ball 35 by the two hemispheres 26 right half 23 of the integrating sphere and an integrating sphere left portions, which are performed by integration of the coupling ball joint 9, and is mounted on the base plate 22 is fixed. 积分球35内壁涂覆漫反射涂层24,对照射到内壁的光线起漫反射匀光的作用。 The inner wall of the integrating sphere 35 coated with diffusely reflective coating 24, the light radiated onto the inner wall acts uniform diffuse reflection of light. 积分球右半部23开有积分球样品孔17,样品室外盖板18 安装在底板22及积分球右半部23上,形成封闭空间样品室21,以消除杂散光的影响。 Right opening portion 23 integrating sphere integrating sphere sample wells 17, sample chamber 18 is mounted on the cover plate 22 and the right half of the integrating sphere 23, a sample chamber 21 form a closed space, in order to eliminate the influence of stray light. 样品室外盖板18上方开有圆孔16,装卸主轴36穿过积分球样品孔17及圆孔16的中心,并与底板22 垂直。 Above the sample chamber 18 with circular opening the cover 16, the spindle 36 through the central handling of the sample wells 17 and the integrating sphere apertures 16 and 22 perpendicular to the base plate. 积分球左半部26开有积分球光源孔28及积分球光纤接口8 (注:本实施例中为SMA905 接口)。 Left portion of the integrating sphere integrating sphere 26 is opened and the aperture 28 of the integrating sphere optical interface 8 (Note: SMA905 the present embodiment is the interface).

[0043] Y形同轴光纤43由激光发射光纤45与信号接收光纤46组成,两者汇聚成一根光纤, 其光纤端面为同轴分布,中心圆形区域为激光发射光纤排列48,外圈同心圆环区域为信号接收光纤排列4"7,该几何配置可高效接收红外激光激发的后向拉曼散射信号以及紫外激光激发的后向荧光信号。Y形同轴光纤43与光纤准直镜42耦合可实现准直发射与接收,光纤准直镜42通过物镜联接器41与显微物镜40相联接,可将红外及紫外激光束聚焦至光谱测试点38并对光谱测试点38的后向散射信号进行收集。物镜支架39将显微物镜40固定安装于底板22上。 [0043] Y-shaped laser light emitted from the optical fiber 43 coaxially with the optical fiber 45 and the optical signal receiver 46, both of converging an optical fiber distribution of the fiber end faces are coaxial, circular central region 48 of the laser emitting optical fiber arrangement, the outer concentric receiving fibers annular region 4 a signal arrangement "7, the geometrical configuration of the received infrared laser can be efficiently excited to the Raman scattering signal and the UV laser excitation to the fluorescent signal .Y shaped coaxial optical fiber 43 and the collimator lens 42 after the coupling may realize transmission and reception of the collimator, the optical fiber collimator 42 coupled to coupler 41 through the objective lens and a microscope objective 40, the infrared and ultraviolet laser beam may be focused to a point spectroscopy spectra points 38 and 38 of the backscatter signal collection. 39 a microscope objective lens holder 40 fixedly mounted on the bottom plate 22.

[0044] Y形光纤51有两个输入端及一个输出端,两个输入端分别联接红外窄线宽激光器49与紫外窄线宽激光器52,输出端通过第二光纤联接器50与Y形同轴光纤43中的激光发射光纤45相联接。 [0044] Y shaped fiber 51 has two input terminals and an output terminal, two input terminals respectively coupled to 4952, the output of the infrared narrow linewidth laser and ultraviolet laser narrow linewidth through the second optical coupler 50 and Y is tantamount the axis of the fiber 43 coupled to laser 45 emitting fiber. Y形光纤51可将红外窄线宽激光器40发出的红外激光与紫外窄线宽激光器52发出的紫外激光同时汇集到激光发射光纤45中。 UV laser and infrared laser narrow linewidth laser 52 emits ultraviolet Y-shaped fiber 51 may be an infrared narrow linewidth laser 40 emitted laser light emission simultaneously aggregated to fiber 45.

[0045]可见谱段光谱仪2与红外谱段光谱仪3均采用相同光谱仪光纤接口4 (注:本实施例中为SMA9〇5接口),两进两出光纤5分为两个接收端(g卩两进)及两个输出端(g卩两出)。 [0045] 2 the visible spectral range and an infrared spectrometer spectral spectrometer spectrometer 3 use the same optical interface 4 (note: in this embodiment is SMA9〇5 Interface), into two optical fiber 5 is divided into two two receiving ends (g Jie two feed) and two outputs (g Jie two). 一个接收端与积分球光纤接口8联接,可收集来自接收光轴20的漫反射光线;另一个接收端与信号接收光纤站通过第一光纤联接器44联接(注:本实施例中为SMA905联接器),可收集来自信号接收光纤46的拉曼及荧光信号;两个输出端分别与可见谱段光谱仪2及红外谱段光谱仪3联接。 A receiving end and an integrating sphere coupled to optical interface 8 may be collected from the diffuse light receiving axis 20; the other end of the signal received by the receiving station 44 coupled to a first fiber optical coupler (Note: SMA905 the present embodiment is coupled devices), can collect the Raman signal and fluorescence signal from the receiving optical fiber 46; and the two output terminals are the visible spectral range and infrared absorption spectrometer 2 3 spectrometer coupled segments.

[0046]超连续谱激光器34 (注:本实施例中其光谱范围400nm-2400nm,功率1W,重频1MHz, 脉宽150ps)发出的可见及红外谱段超连续谱脉冲激光经激光器尾纤32传输,然后通过光纤准直接头31进行光束准直后输出超连续谱脉冲准直激光束,并沿发射光轴25穿过积分球光源孔28进入积分球35。 [0046] supercontinuum laser 34 (Note: In this example the spectral range 400nm-2400nm, the present embodiment 1W power, repetition frequency 1MHz, pulse width 150ps) visible and infrared spectral range emitted by the pulsed laser supercontinuum laser pigtail 32 transmission, then the output 31 over the collimated beam after quasi continuous spectrum through an optical fiber directly head pulsed collimated laser beam, and the light source 25 passes through the hole 28 into the integrating sphere integrating sphere 35 along the emission optical axis.

[0047]光纤准直接头31由支架29固定在底板22上,并通过光源室外盖板30与积分球左半部26外壳联接,形成封闭空间光源室27,以消除杂散光的影响。 [0047] Alignment optical head 31 is fixed on the base plate 29 by a bracket 22, and coupled by a light source outside the housing 26 and the cover 30 integrating sphere left portion, a light source chamber 27 form a closed space, in order to eliminate the influence of stray light.

[0048]红外窄线宽激光器49发出的红外连续激光束经Y形光纤51至Y形同轴光纤43中的激光发射光纤45传输、从激光发射光纤排列48中发射后沿激光光轴37传输、经光纤准直镜42准直、显微物镜40聚焦后可实现对光谱测试点38的红外诱导拉曼激发(注:激光光轴37与装卸主轴36相交,其交点为光谱测试点38),光谱测试点38的后向散射拉曼信号依次经显微物镜40及光纤准直镜42后由Y形同轴光纤43端面的外圈同心圆环区域,即信号接收光纤排列47收集,再经信号接收光纤46、两进两出光纤5至红外谱段光谱仪3进行接收和分析。 [0048] The continuous infrared laser beam emitted infrared narrow linewidth laser emission 49 through the transmission fiber 45 in the Y-shaped fiber 51 coaxially to the Y-shaped laser fiber 43, laser light emitted from the optical fiber 37 are arranged in the transmission along the laser emission axis 48 , the optical fiber collimator lens 42 collimating, focusing the microscope objective 40 may be implemented after the infrared spectra induced Raman excitation point 38 (Note: the laser optical axis 36 intersects the main shaft 37, loading and unloading, the intersection point 38 as spectra) after spectral test point 38 through the microscope objective 40 and the outer concentric annular region 42 by a Y-shaped optical fiber 43 coaxially with the fiber end face of the collimator lens, i.e., the Raman backscatter signal received signals are sequentially collected by the optical fiber 47 are arranged, and then 46, two optical fibers 5 into two spectral bands to an infrared spectrometer 3 receives and analyzes the signal received via the optical fiber.

[0049]紫外窄线宽激光器52发出的紫外连续激光束经Y形光纤51至Y形同轴光纤43中的激光发射光纤祁传输、从激光发射光纤排列4S中发射后沿激光光轴37传输、经光纤准直镜42准直、显微物镜40聚焦后可实现对光谱测试点38的紫外诱导荧光激发,光谱测试点38的后向散射荧光信号依次经显微物镜40及光纤准直镜似后由Y形同轴光纤43端面的外圈同心圆环区域,即信号接收光纤排列47收集,再经信号接收光纤46、两进两出光纤5至可见谱段光谱仪2进行接收和分析。 [0049] UV ultraviolet laser beam continuously narrow linewidth laser emission 52 emitted by the optical fiber transmission Qi Y to Y-shaped fiber 51 coaxially with the laser fiber 43, laser light emitted from the optical fiber 37 are arranged in the transmission along the laser emission axis 4S , the optical fiber collimator lens 42 collimating, focusing the microscope objective 40 may be implemented after ultraviolet spectra induced fluorescence excitation node 38, the node 38 spectra backscattered fluorescence signals sequentially through the microscope objective 40 and the optical fiber collimator lens after the like, i.e., signals received by the collecting fiber 47 arranged concentric annular outer area of ​​the end face 43 of the Y-shaped fiber coaxial, and then receives the signal fiber 46, two optical fibers 5 into two sections to the visible spectrum spectrophotometer 2 receives and analyzes.

[0050]导轨12与底板22垂直安装,试管机械手1〇通过连接杆n与导轨12联接并可在机械手运动控制器13控制下沿导轨12滑动。 [0050] The guide rails 12 and the base plate 22 mounted vertically, and the test tube under 1〇 robot manipulator 13 controls the movement of the controller 12 slides along the guide rail by a connecting rod 12 coupled to the guide rail n. 待检血液i 9由试管盖i 4封装在试管i 5中。 Be examined by a blood test tube caps i 9 i 4 i 5 are encapsulated in a tube. 试管机械手10在机械手运动控制器13控制下,可抓紧试管盖14并带动试管15沿装卸主轴36方向上下运动。 Manipulator tube 10 under the control of the robot motion controller 13, the lid 14 can grasp the tube and drive tube 15 in the loading direction of the main shaft 36 up and down.

[0051]电源模组1用以对可见谱段光谱伩2、红外谱段光谱仪3、主控及数据分析系统7、超连续谱激光器34、红外窄线宽激光器49、紫外窄线宽激光器52及机械手运动控制器13进行供电。 [0051] The power module 1 for the visible spectral spectrum Xin 2, infrared spectral spectrometer 3, the main control system 7 and data analysis, supercontinuum laser 34, an infrared narrow linewidth lasers 49, narrow linewidth laser 52 UV the motion controller 13 and the robot power supply. 主控及数据分析系统7用以对可见谱段光谱伩2、红外谱段光谱仪3、超连续谱激光器34、红外窄线宽激光器40、紫外窄线宽激光器52及机械手运动控制器13进行控制,并通过USB接口6接收可见谱段光谱仪2及红外谱段光谱仪3输出的光谱数据进行处理和分析。 7 master and data analysis system for the visible spectral spectrum Xin 2, 3 IR spectroscopy spectral supercontinuum laser 34, an infrared narrow linewidth laser 40, an ultraviolet narrow linewidth laser 52 and the robot controller 13 controls the movement and processing and analyzing the USB interface 6 receives the visible spectral spectrometer 2 spectrum data and infrared spectral output from the spectrometer through. 主控及数据分析系统7内含触摸屏人机交互界面33,用于与用户的人机交互,接受用户的指令并输出结果给用户。 7 and master data analysis system containing the touch screen interactive interface 33 for human interaction with a user, receiving a user's instruction and outputs the result to the user.

[0052]基于红外拉曼、紫外荧光结合超连续漫综合激光光谱的密封血液鉴别仪的血液分析方法其步骤为: A blood analysis method of sealing a blood Discriminator [0052] Infrared Raman, UV fluorescence binding supercontinuum integrated diffuse the laser spectrum comprises the following steps:

[0053] (1)仪器启动及超连续谱测试进样 [0053] (1) Start the instrument and the test sample supercontinuum

[0054]开启电源模组1,对可见谱段光谱伩2、红外谱段光谱仪3、主控及数据分析系统7、 超连续谱激光器34、红外窄线宽激光器49、紫外窄线宽激光器52及机械手运动控制器13进行供电。 [0054] The power module 1 is turned on, the visible spectral spectrum Xin 2, infrared spectral spectrometer 3, the main control system 7 and data analysis, supercontinuum laser 34, an infrared narrow linewidth lasers 49, narrow linewidth laser 52 UV the motion controller 13 and the robot power supply.

[0055]用户通过触摸屏人机交互界面33启动测试主程序。 [0055] the user through the touch screen interactive interface 33 to start the test the main program. 此时,主控及数据分析系统7发出进样指令给机械手运动控制器13,机械手运动控制器13控制试管机械手10抓取试管15进样至其沿装卸主轴36方向进入积分球35。 At this time, the main control system 7 and data analysis sample issue instructions to the robot motion controller 13, motion controller 13 controls the robot manipulator tube 10 to the injector tube 15 gripping it enters the integrating sphere 35 along the loading direction of the spindle 36. 试管15底部位置高于发射光轴25且与接收光轴20 不相交,避免激光束直接照射待检试管15,并避免试管15的透反射光线直接沿发射光轴25 传输并被两进两出光纤5收集。 A position higher than a bottom-emitting tube 15 and the optical axis 25 does not intersect with the light receiving axis 20, to avoid direct exposure to the laser beam tube 15 to be tested, and to avoid light reflection through the tube 15 directly along the emission optical axis and two transmission 25 into two fiber 5 was collected.

[0056] (2)超连续谱激光漫综合光谱测试 [0056] (2) a supercontinuum laser integrated diffuse spectra

[0057]如图la所示,主控及数据分析系统7发出指令启动超连续谱激光器34、可见谱段光谱仪2及红外谱段光谱仪3。 [0057] As shown in FIG La, and the master data analysis system issues a command to start 7 supercontinuum laser 34, the visible spectral and infrared absorption spectrometer 2 section 3 spectrometer. 超连续谱激光器34输出的超连续谱准直激光束沿发射光轴25进入积分球35,激光束照射到漫反射涂层24后,其反射光为漫反射,g卩在积分球35内沿各方向传输,变成了均匀光线。 Supercontinuum laser 34 outputs supercontinuum collimated laser beam 25 enters the integrating sphere 35 along the optical axis of the emitted laser beam is irradiated to the diffusely reflecting coating 24, the reflected light is reflected diffusely, g Jie in an integrating sphere 35 along the each direction of transmission, into a uniform light. 试管15受不同方向的超连续谱光线照射后,试管15 (注:包括其材质与外贴标签)与待检血液19的漫反射、漫透射、吸收、发射后的光线将沿空间任意方向传输, 遇到漫反射涂层24漫反射至任意方向,其对积分球35内的光线传输具有漫综合光谱影响。 After the tube supercontinuum light irradiation of different directions 15, tube 15 (Note: include the material of the outer label) to be the subject of blood diffuse reflection 19, diffuse transmission, absorption of light after transmitting the transmission in any direction of space, diffusely reflective coating 24 face diffusely reflected to any direction, which has a diffuse light transmission integrated spectral effect on the integrating sphere 35. [0058]沿发射光轴25传输的漫反射光线被两进两出光纤5收集后,分别送至可见谱段光谱伩2及红外谱段光谱仪3进行光电转换变成光谱数据。 After the [0058] two are collected into two fibers 5 in diffuse light emitting optical axis 25 of the transmission, respectively, to the visible spectral spectrum and infrared spectral Xin 2 spectrometer 3 is photoelectrically converted into spectral data. 本具体实施例中可见谱段光谱仪2 的光谱范围为370-750nm,采样点为Nl = 1300。 Examples spectral visible spectral range of the spectrometer 2 370-750nm, sample points Nl = 1300 this particular embodiment. 红外谱段光谱仪3的光谱范围为800_1750nm, 采样点为N2 = 512。 Infrared spectral spectrometer for the spectral range of 3 800_1750nm, sampling point is N2 = 512. 可见谱段光谱仪2及红外谱段光谱仪3输出的共N=N1+N2个光谱数据经USB接口6送至主控及数据分析系统7进行存储。 2 the visible spectral spectroscopy and infrared spectral co-N = N1 + N2 spectrum data at the output of the spectrometer 3 to the host via the USB interface 6 and 7 store the data analysis system.

[0059] ⑶拉曼荧光光谱测试进样 [0059] ⑶ Raman spectroscopy sample fluorescence

[0060]主控及数据分析系统7发送出样指令给机械手运动控制器13,机械手运动控制器13控制试管机械手10带动试管15至其沿装卸主轴36方向移出积分球35及样品室21,至光谱测试点38位于试管15内待检血液19的中心位置,此时完成拉曼荧光光谱测试进样。 [0060] The master control and data analysis system 7 transmits the like instructions to the robot motion controller 13, the robot motion controller 13 controls the tube manipulator 10 to drive tube 15 to move it out of the integrating sphere 35 and the sample chamber 21 in the 36 direction of loading of the spindle, to spectroscopy to be 38 point 15 is located within the tube 19 of the center position of the blood sample, Raman This completes the test sample fluorescence spectroscopy.

[0061] ⑷红外激光拉曼及紫外激光荧光光谱测试 [0061] ⑷ infrared laser Raman laser fluorescence and UV spectra

[0062]如图1b所示,主控及数据分析系统7发出指令启动红外窄线宽激光器49、紫外窄线宽激光器52、可见谱段光谱仪2及红外谱段光谱仪3。 [0062] Figure 1b, the master data analysis system 7 and issues an instruction to start an infrared narrow linewidth lasers 49, narrow linewidth laser 52 UV, visible spectral bands and infrared absorption spectrometer 2 section 3 spectrometer.

[0063]紫外窄线宽激光器52(注:本实施例中采用波长为36〇nm±lnm,功率0.05W的半导体泵浦固体连续激光器)发出的紫外连续激光束经准直聚焦后会聚到光谱测试点38处的待检血液19,紫外窄线宽连续激光所诱导的荧光光谱信号依次经显微物镜4〇及光纤准直镜42 后由Y形同轴光纤43端面的外圈同心圆环区域,即信号接收光纤排列47收集,再经信号接收光纤46、两进两出光纤5至可见谱段光谱仪2进行光电转换变成光谱数据,本具体实施例中可巧谱段光谱仪2的光谱范围为37〇_75〇nm,对荧光光谱采用与超连续谱测试可见谱段一样的采样位置与采样点数,g卩N3 = N1 = 1:300。 [0063] UV narrow linewidth laser 52 (Note: In this example a wavelength of 36〇nm ± lnm, 0.05W power diode pumped solid continuous laser of the present embodiment) after the ultraviolet laser beam continuously emitted collimated by focusing a straight converged spectrum blood to be tested at a test point 3819, UV narrow linewidth continuous laser induced fluorescence spectrum signals are sequentially via the outer ring and the microscope objective 4〇 Y-shaped fiber end face 43 is coaxial with the optical fiber collimator 42 concentric rings region, i.e., signal receiving fiber arrangement 47 to collect, and then receiving the optical fiber 46, two-two optical fibers 5 to the visible spectral spectrometer 2 is photoelectrically converted into spectral data by the signal spectral range embodiment happened that spectral spectrometer 2 of the present specific embodiment It is 37〇_75〇nm, and the fluorescence spectra were obtained using a supercontinuum test the visible spectral sampling positions as sampling points, g Jie N3 = N1 = 1: 300. 由于紫外窄线宽激光器52的波长位于可见谱段光谱仪2的光谱范围之外,因此紫外激光回波不会影响对荧光光谱信号的采集,无需采用瑞利滤光片抑制回波干扰。 Since the wavelength UV narrow linewidth laser 52 is located outside the visible spectral spectrometer spectral range 2, and thus will not affect the ultraviolet laser echo signals acquired on the fluorescence spectrum, suppressed without employing Rayleigh wave interference filters. 可见谱段光谱仪2输出的N3个光谱数据经USB接口6送至主控及数据分析系统7进行存储。 Visible spectral output from the spectrometer N3 spectral data to the host via the USB interface 6 and 7 store the data analysis system.

[00M]同时,红外窄线宽激光器的(注:本实施例中采用波长为785nm±lnm,功率〇.3_ 半导体泵浦固体连续激光器)发出的红外连续激光束经准直聚焦后会聚到光谱测试点38处的待检血液I9,红外窄线宽连续激光所诱导的斯托克斯拉曼光谱信号依次经显微物镜40及光纤准直镜42后由Y形同轴光纤43端面的外圈同心圆环区域,即信号接收光纤排列47收集, 再经信号接收光纤46、两进两出光纤5至红外谱段光谱仪3进行光电转换变成光谱数据,本具体实施例中红外谱段光谱仪3的光谱范围为800-1750nm,对拉曼光谱采用与超连续谱测试红外谱段一样的采样位置与采样点数,即N4=N2 = 512。 [00M] Meanwhile, the infrared narrow linewidth lasers (Note: In this example a wavelength of 785nm ± lnm, continuous power 〇.3_ DPSS laser of the present embodiment) after the continuous infrared laser beam collimated by converging the emitted spectrum of the linear focus a Y-shaped outer end face of the optical fiber 43 coaxially with the test after the collimator lens 42 to be examined at a point 38 of blood I9, infrared narrow linewidth continuous laser induced Stokes Raman spectroscopy signals sequentially through the microscope objective 40 and a fiber collimator circle concentric annular region, i.e., signal receiving fiber arrangement 47 to collect, and then receives the optical fiber through a signal 46, two into two optical fibers 5 to the infrared spectral spectrometer 3 is photoelectrically converted into spectral data, for example, infrared spectral bands spectrometer of the present specific embodiment 3 is a spectral range of 800-1750nm, and Raman spectra were obtained using a supercontinuum test infrared spectral sampling positions as sampling points, i.e. N4 = N2 = 512. 由于红外窄线宽激光器49的波长位于红外谱段光谱仪3的光谱范围之外,因此红外激光回波不会影响对拉曼光谱信号的采集,无需采用瑞利滤光片抑制回波干扰。 Since the wavelength of the infrared narrow linewidth laser 49 is positioned outside the infrared spectral spectroscopy spectrum 3, and therefore does not affect the echo infrared laser Raman spectrum signal acquisition, without Rayleigh wave interference suppression filter. 红外谱段光谱仪3输出的N4个光谱数据经USB接口6 送至主控及数据分析系统7进行存储。 Infrared spectral output from the spectrometer N4 spectral data to the host via the USB interface 6 and 7 store the data analysis system.

[0065] (5)数据分析及融合 [0065] (5) Analysis and Data Fusion

[0066]将可见谱段的超连续谱激光漫综合光谱的N1个光谱数据与荧光光谱的N3个光谱数据进行加权叠加,得到N1个可见谱段的融合光谱数据。 [0066] The spectral data of the visible N3 supercontinuum laser integrated spectrum N1 diffuse spectral data of the fluorescence spectrum of the weighted spectral superposition to obtain spectral data of N1 fusion visible spectral ranges. 其中荧光光谱的权重值为F,超连续谱激光漫综合光谱的权重值为1-F。 Wherein the heavy weight of the fluorescence spectrum is F, right supercontinuum laser integrated diffuse spectral weight is 1-F. (注:本实施例中F = 0.7) (Note: In Example F of the present embodiment = 0.7)

[0067] 将红外谱段的超连续谱激光漫综合光谱的N2个光谱数据与拉曼光谱的N4个光谱数据进行加权叠加,得到N2个红外谱段的融合光谱数据。 [0067] The supercontinuum laser diffuse N2 integrated spectrum data of infrared spectra and Raman spectra of spectral N4 of spectral data weighted superposition, the spectral data obtained fused N2 infrared spectral ranges. 其中拉曼光谱的权重值为L,超连续谱激光漫综合光谱的权重值为1-L。 Wherein the heavy weight of the Raman spectrum is L, the right supercontinuum laser integrated diffuse spectral weight is 1-L. (注:本实施例中L=0.4) (Note: this embodiment L = 0.4)

[0068]将N1个可见谱段的融合光谱数据与N2个红外谱段的融合光谱数据组成共N个光谱数据用于后续分析。 [0068] The fused fusion spectrum visible spectral data of N1 and N2 spectral infrared spectral data consisting of a total of N spectral data for subsequent analysis. 基于主成分分析法(principal components analysis,简称PCA)计算这N个光谱数据的M个主成分数值(注:在本实施例中M=7,即计算7个主成分数值),进行降维处理。 Calculating the N spectral data based on principal component analysis (principal components analysis, referred to as PCA) M principal component values ​​(Note: In the present embodiment, M = 7, i.e. calculate seven principal component values), dimensionality reduction .

[0069] ⑹血液鉴别与判定 [0069] ⑹ Identification and determination of blood

[0070] 将待检血液19的M个主成分数值,得到其在M维主成分空间的特征向量,将该特征向量与M维主成分空间的由本血液鉴别仪获得的人与动物全血、血浆、血清M维主成分空间数据库的聚类中心特征向量进行对比,根据特征向量相似度首先确定待检血液19的血液类型,即是全血、血浆还是血清。 [0070] to be tested Blood M principal component values ​​of 19 to obtain feature vectors in the M-dimensional principal component space, and the person is obtained by the present blood identification device with the animal eigenvectors and M-dimensional principal component space of the whole blood, plasma, serum cluster center M-dimensional feature vector space of the main component of the database by comparing feature vector similarity blood in the blood to be tested is first determined according to the type 19, i.e., whole blood, plasma or serum. 然后,再将待检血液19的M个主成分数值,与该血液类型下的人与动物不同种属的M维主成分空间数据库的聚类中心特征向量进行对比,根据特征向量相似度再确足种属,即是人还是动物血液,如果是动物血液,是何种动物血液,至此,完成待检血液19的血液类型与种属的判定。 Then, the blood to be tested and then principal component values ​​of M of 19, the cluster centers feature vector with a different species of the genus of animal and human blood in this type of an M-dimensional principal component space database are compared, and then determines the feature vector similarity full species, namely human or animal blood, animal blood if it is, what kind of animal blood, thus, complete blood type and blood to be tested 19 species of the genus judgment.

[0071]然后,主控及数据分析系统7将待检血液19的红外激光诱导拉曼光谱紫外激光诱导荧光光谱及可见红外超连续漫综合激光光谱的光谱曲线以及判定结果显示在触摸屏人机交互界面33上,以供用户参考。 [0071] Then, the main control system 7 and data analysis of blood to be tested 19 is an infrared laser-induced Raman spectrum UV laser induced fluorescence spectroscopy in the visible spectrum and IR curve of a supercontinuum laser spectroscopy and diffuse comprehensive judgment result display interactive touchscreen the interface 33, for user reference. 至此完成整个测试过程。 This completes the entire testing process.

Claims (1)

1.一种基于红外拉曼、紫外荧光结合超连续漫综合激光光谱的密封血液鉴别仪器,它包括电源模组(1)、可见谱段光谱仪(2)、红外谱段光谱仪⑶、两进两出光纤(5)、主控及数据分析系统(7)、积分球(35)、试管机械手(10)、连接杆(丨丨)、导轨(12)、机械手运动控制器(13)、样品室外盖板(18)、底板(22)、支架(29)、光源室外盖板(30)、光纤准直接头(31)、激光器尾纤C32)、超连续谱激光器CB4)、物镜支架〇39)、显微物镜(40)、物镜联接器(41)、光纤准直镜(4¾、Y形同轴光纤(43)、第一光纤联接器(44)、第二光纤联接器(50)、Y形光纤(51)、 红外窄线宽激光器(49)、紫外窄线宽激光器(52),其特征在于: 所述的积分球(35)由积分球右半部(23)和积分球左半部(26)两个半球组成,它们通过积分球接头(9)进行联接,并安装固定在底板(22)上;积分球(35)内壁涂覆漫反射涂层(24),对 An infrared-based Raman, UV fluorescence binding supercontinuum laser spectroscopy diffuse integrated differential blood sealing instrument which includes a power supply module (1), the visible spectral spectrometer (2), infrared spectroscopy spectral ⑶, two-two an optical fiber (5), the main control and data analysis system (7), an integrating sphere (35), the tube manipulator (10), a connecting rod (Shushu), the guide rail (12), the robot motion controller (13), sample chamber the cover (18), the bottom plate (22), the bracket (29), a light source outside the cover (30), the quasi-direct optical head (31), a laser pigtail C32), CB4 supercontinuum laser), lens holder 〇39) , microscope objective (40), coupling lens (41), an optical fiber collimator (4¾, Y-shaped fiber coaxial (43), a first optical coupler (44), a second optical coupler (50), Y shaped fiber (51), an infrared narrow linewidth lasers (49), an ultraviolet narrow linewidth lasers (52), characterized in that: said integrating sphere (35) by the right half integrating sphere (23) and an integrating sphere left (26) two hemispheres, they are coupled by integrating a ball joint (9), mounted and fixed to the base (22); integrating sphere (35) an inner wall coated with a diffuse reflective coating (24), of 照射到内壁的光线起漫反射匀光的作用;积分球右半部(23)开有积分球样品孔(I7),样品室外盖板(18)安装在底板(2¾及积分球右半部(23)上,形成封闭空间样品室(21),以消除杂散光的影响;样品室外盖板(1S)上方开有圆孔(16),装卸主轴(36)穿过积分球样品孔(17)及圆孔(16)的中心,并与底板(22)垂直;积分球左半部(26)开有积分球光源孔(28)及积分球光纤接口(8); 所述的Y形同轴光纤(4¾由激光发射光纤(45)与信号接收光纤(46)组成,两者汇聚成一根光纤,其光纤端面为同轴分布,中心圆形区域为激光发射光纤排列(48),外圈同心圆环区域为信号接收光纤排列(47),该几何配置可高效接收红外激光激发的后向拉曼散射信号以及紫外激光激发的后向荧光信号;Y形同轴光纤(4¾与光纤准直镜(42)耦合可实现准直发射与接收,光纤准直镜(42)通过物镜联接器(41)与显微物镜(40 Light is irradiated to the inner wall of the role played diffuse reflection of light absorbed; integrating sphere right portion (23) opening with an integrating sphere sample well (I7), sample chamber cover (18) mounted on the base plate (2¾ integrating sphere and a right half ( 23), form a closed space of the sample chamber (21), in order to eliminate the influence of stray light; sample chamber cover (1S) above the circular opening (16), detachably spindle (36) passes through the integrating sphere sample well (17) and center circular hole (16), and the bottom plate (22) vertically; integrating sphere left portion (26) opening with an integrating sphere light source apertures (28) and integrating sphere optical interface (8); Y-shape of the coaxial fiber (4¾ emitted by the laser fiber (45) and the signal receiving fiber (46), both of converging an optical fiber distribution of the fiber end faces are coaxial, the central circular zone emitting fiber arrangement (48) is a laser, a concentric outer ring receiving fibers arranged annular region (47) of the signal, the geometrical configuration of the received infrared laser can be efficiently excited to the fluorescent signal to the Raman scattering signal and the UV laser excitation; Y stands shaped fiber coaxial (with the optical fiber collimator lens 4¾ (42) coupled to the collimator may be implemented transmit and receive, the optical fiber collimator lens (42) by a coupling lens (41) and microscope objective (40 )相联接,可将红外及紫外激光束聚焦至光谱测试点(38)并对光谱测试点(38)的后向散射信号进行收集;物镜支架(39)将显微物镜(40)固定安装于底板(22)上; 所述的Y形光纤(51)有两个输入端及一个输出端,两个输入端分别联接红外窄线宽激光器(49)与紫外窄线宽激光器(5¾,输出端通过第二光纤联接器(50)与Y形同轴光纤(43) 中的激光发射光纤(45)相联接;Y形光纤(51)可将红外窄线宽激光器(49)发出的红外激光与紫外窄线宽激光器(52)发出的紫外激光同时汇集到激光发射光纤(45)中; 所述的可见谱段光谱仪(2)与红外谱段光谱仪(3)均采用相同光谱仪光纤接口(4),两进两出光纤(5)分为两个接收端及两个输出端;一个接收端与积分球光纤接口(8)联接,可收集来自接收光轴(20)的漫反射光线;另一个接收端与信号接收光纤(46)通过第一光纤联接器(44)联接,可收集来自信号接收光 ) Coupled to, and may be an infrared spectrum UV laser beam is focused to a test point (38) and spectroscopy test point (38) after the collected backscattered signal; an objective lens holder (39) the microscope objective (40) fixedly mounted to the bottom plate (22); said Y-shaped fiber (51) has two input terminals and an output terminal, two input terminals respectively coupled to the infrared narrow linewidth lasers (49) with an ultraviolet laser narrow linewidth (5¾, an output terminal Y shaped fiber infrared laser (51) may be an infrared narrow linewidth lasers (49) and emitted; coupled emitting fiber (45) through a second fiber optic connector (50) coaxial with the Y-shaped optical fiber (43) a laser UV laser UV narrow linewidth lasers (52) emitted simultaneously together to laser light transmitting fiber (45); the visible spectral range spectrometer (2) infrared spectral spectrometer (3) use the same spectrometer optical interface (4) , two into two optical fibers (5) into two receiving ends and two output ports; a receiving end and an integrating sphere optical interface (8) is coupled, may be collected from the diffuse light receiving axis (20); the other signal receiving fiber receiving end (46) (44) is coupled, can be collected by a first optical signal received from the fiber optic coupler (妨)的拉曼及荧光信号;两个输出端分别与可见谱段光谱仪(2)及红外谱段光谱仪(3)联接; 所述的超连续谱激光器(34)发出的可见及红外谱段超连续谱脉冲激光经激光器尾纤(32)传输,然后通过光纤准直接头(31)进行光束准直后输出超连续谱脉冲准直激光束,并沿发射光轴(25)穿过积分球光源孔(28)进入积分球(35); 所述的光纤准直接头(31)由支架(29)固定在底板(22)上,并通过光源室外盖板(30)与积分球左半部(26)外壳联接,形成封闭空间光源室(27),以消除杂散光的影响; 所述的红外窄线宽激光器(4¾发出的红外连续激光束经Y形光纤(51)至y形同轴光纤(43)中的激光发射光纤(45)传输、从激光发射光纤排列(4¾中发射后沿激光光轴(37)传输、经光纤准直镜(42)准直、显微物镜(40)聚焦后可实现对光谱测试点(38)的红外诱导拉曼激发,光谱测试点(38)的后向散射拉曼信号依次 (Hinder) Raman and fluorescence signals; two output terminals respectively spectrometer the visible spectral range (2) and infrared absorption spectroscopy section (3) is coupled; the visible and infrared spectral supercontinuum laser (34) emitted supercontinuum laser pigtail by a pulsed laser (32) transmission, and the output light beam collimated pulsed supercontinuum laser beam through an optical fiber collimated Alignment head (31) and (25) passing through the sphere along the emission optical axis of light source apertures (28) into the integrating sphere (35); Alignment of the fiber optic head (31) is fixed by the bracket (29) on the base plate (22), and by a light source outside the cover plate (30) and the left half of the integrating sphere (26) coupled to the housing, the light source chamber form a closed space (27), in order to eliminate the influence of stray light; coaxially to the y-continuous infrared laser beam of the infrared laser narrow linewidth (4¾ emitted by the Y-shaped optical fiber (51) fiber laser (43) of the transmitting fiber (45) transmission, transmitting the fiber arrangement ((37) after transmission along the optical axis of the laser emitted from the laser in 4¾ via an optical fiber collimator lens (42) collimates microscope objective (40) after focusing is achieved on IR spectroscopy point (38) induced Raman excitation, the spectrum of the test points (38) of the forward scattered Raman signal sequentially 显微物镜(40)及光纤准直镜(42)后由Y形同轴光纤(4¾端面的外圈同心圆环区域,即信号接收光纤排列(47)收集,再经信号接收光纤(46)、两进两出光纤⑸至红外谱段光谱仪⑶进行接收和分析; 所述的紫外窄线宽激光器(52)发出的紫外连续激光束经Y形光纤(51)至Y形同轴光纤(4¾中的激光发射光纤(4¾传输、从激光发射光纤排列(48)中发射后沿激光光轴(37)传输、经光纤准直镜(4¾准直、显微物镜(40)聚焦后可实现对光谱测试点(38)的紫外诱导荧光激发,光谱测试点(38)的后向散射荧光信号依次经显微物镜(40)及光纤准直镜(42)后由Y形同轴光纤(43)端面的外圈同心圆环区域,即信号接收光纤排列(47)收集,再经信号接收光纤(46)、两进两出光纤(5)至可见谱段光谱仪(2)进行接收和分析; 所述的导轨(1¾与底板(22)垂直安装,试管机械手(10)通过连接杆(11)与导轨(12)联接并可 Microscope objective (40) and the collimator lens after the optical fiber (42) by a Y-shaped fiber coaxial (concentric annular outer end surface 4¾ region, i.e., signal receiving fiber arrangement (47) were collected, and then the signal receiving fiber (46) , two ⑸ into two optical fibers to the infrared spectral spectrometer for receiving and analyzing ⑶; ultraviolet a continuous laser beam of the ultraviolet narrow linewidth lasers (52) emitted by the Y-shaped optical fiber (51) to a Y-shaped fiber coaxial (4¾ the laser light transmitting fiber (4¾ transmission arrangement emitting fiber (from laser 48) (37) after transmission along the optical axis of the laser emitted by the optical fiber collimating lens (collimator 4¾, microscope objective (40) after focusing is achieved spectra point (38) of ultraviolet induced fluorescence excitation spectra of the test points (38) sequentially to the fluorescent signal scatter through the microscope objective (40) and an optical fiber collimator lens (42) after the Y-shaped fiber coaxial (43) concentric annular outer end surface area, i.e., signal receiving fiber arrangement (47) were collected, and then receiving fiber (46) via a signal, two-two optical fibers (5) to the visible spectral range spectrometer (2) for receiving and analysis; the the guide rail (1¾ said bottom plate (22) mounted vertically, the manipulator tube (10) by a connecting rod (11) the guide rail (12) is coupled and 在机械手运动控制器(13)控制下沿导轨(I2)滑动;待检血液(19)由试管盖(14)封装在试管(15)中;试管机械手(10)在机械手运动控制器(13)控制下,可抓紧试管盖(M)并带动试管(15)沿装卸主轴(36)方向上下运动; 所述的电源模组(1)用以对可见谱段光谱仪(2)、红外谱段光谱仪(3)、主控及数据分析系统(7)、超连续谱激光器(34)、红外窄线宽激光器(49)、紫外窄线宽激光器(52)及机械手运动控制器(1¾进行供电;主控及数据分析系统(7)用以对可见谱段光谱仪(2)、红外谱段光谱仪(3)、超连续谱激光器(34)、红外窄线宽激光器(49)、紫外窄线宽激光器(52)及机械手运动控制器(1¾进行控制,并通过USB接口(6)接收可见谱段光谱仪(2)及红外谱段光谱仪(3)输出的光谱数据进行处理和分析;主控及数据分析系统(7)内含触摸屏人机交互界面(33),用于与用户的人机交互,接受用户的指令 In the robot motion controller (13) under control along the guide rail (I2) slide; to be examined in blood (19) by a tube cap (14) enclosed in a tube (15); tube robot (10) in the robot motion controller (13) under the control of, can grasp the cover tube (M) and drive tube (15) along the loading axis (36) moved up and down direction; said power module (1) for the visible spectral range of the spectrometer (2), infrared spectroscopy spectral (3), and the master data analysis system (7), supercontinuum laser (34), an infrared narrow linewidth lasers (49), an ultraviolet narrow linewidth lasers (52) and the robot motion controller (1¾ supplying power; main control and data analysis system (7) for the visible spectral spectrometer (2), infrared spectral spectrometer (3), supercontinuum laser (34), an infrared narrow linewidth lasers (49), narrow linewidth laser UV ( 52) and the robot motion controller (1¾ controls, via the USB interface (6) receiving the visible spectral spectrometer (2) and infrared spectral spectrometer (3) spectral data output processing and analysis; master control and data analysis system (7) containing man-machine interface touch screen (33) for human interaction with a user, receiving a user's instruction 输出结果给用户; 主控及数据分析系统发出指令启动超连续谱激光器、可见谱段光谱仪及红外谱段光谱仪,超连续谱激光器输出的超连续谱准直激光束沿发射光轴进入积分球,激光束照射到漫反射涂层后,其反射光为漫反射,试管受不同方向的超连续谱光线照射后,试管、试管外贴标签与待检血液的漫反射、漫透射、吸收、发射后的光线将沿空间任意方向传输,遇到漫反射涂层漫反射至任意方向,其对积分球内的光线传输具有漫综合光谱影响;沿发射光轴传输的漫反射光线被两进两出光纤收集后,分别送至可见谱段光谱仪及红外谱段光谱仪进行光电转换变成光谱数据;主控及数据分析系统发出指令启动红外窄线宽激光器、紫外窄线宽激光器、可见谱段光谱仪及红外谱段光谱仪;紫外窄线宽激光器发出的紫外连续激光束经准直聚焦后会聚到光谱测试 Outputs the result to the user; master control and data analysis system issues a command to start supercontinuum laser, the visible spectral spectroscopy and infrared spectral spectrometer, supercontinuum collimated laser supercontinuum laser output beam along the emission optical axis enters the integrating sphere, a laser beam is irradiated to the diffusely reflecting coating which reflects light diffuse reflection, the tube supercontinuum light irradiation of different directions, the test tube, an outer tube labeling to be diffusely reflecting object blood, diffuse transmission, absorption, emission after transmitting light in any direction of space, diffuse reflection coating face diffusely reflected to any direction, which has a diffuse effect on the spectral transmission of light integrated in the integrating sphere; two are collected into two fibers diffuse light emitted along the optical axis of the transmission after, respectively, to the visible and infrared spectral spectrometer spectral spectrometer photoelectric conversion into spectral data; master control and data analysis system issues a command to start an infrared narrow linewidth lasers, narrow linewidth laser ultraviolet, visible and infrared spectral spectrometer spectrum segment spectrometer; UV ultraviolet narrow linewidth laser beam continuously emitted by the laser collimator focusing converging spectra 处的待检血液,紫外窄线宽连续激光所诱导的荧光光谱信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至可见谱段光谱仪进行光电转换变成光谱数据;红外窄线宽激光器发出的红外连续激光束经准直聚焦后会聚到光谱测试点处的待检血液,红外窄线宽连续激光所诱导的斯托克斯拉曼光谱信号依次经显微物镜及光纤准直镜后由Y形同轴光纤端面的外圈同心圆环区域,即信号接收光纤排列收集,再经信号接收光纤、两进两出光纤至红外谱段光谱仪进行光电转换变成光谱数据;可见谱段光谱仪及红外谱段光谱仅知出的光谱数据经USB接口送至主控及数据分析系统进行存储、分析处理。 The blood to be tested, an ultraviolet continuous narrow linewidth laser induced fluorescence at the spectroscopic signals sequentially via a microscope objective lens and an optical fiber collimated by the Y-shaped fiber ends coaxially concentric annular outer area, i.e., an optical fiber signal receiver arranged to collect , then the received optical signal by two optical fibers into two spectral bands visible spectrometer to photoelectrically converted into spectral data; continuous infrared laser beam emitted from the infrared narrow linewidth laser focused through converging collimator to be examined at the test point spectrum blood, infrared narrow linewidth continuous laser induced Stokes Raman spectroscopy signals sequentially via a microscope objective lens and a fiber collimator straight rear end face of the Y-shaped fiber coaxial outer concentric annular region, i.e., signal receiving fiber arrangement were collected, and then the received optical signal, into the two optical fibers to two infrared spectrometer spectral photoelectric conversion into spectral data; spectrometer the visible spectral spectrum and the spectral data known only infrared spectral range to the host via the USB interface and data analysis system for storage, analysis and processing.
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