CN105628679B - Blood identifier based on infrared Raman Ultraluminescence super continuous spectrums - Google Patents
Blood identifier based on infrared Raman Ultraluminescence super continuous spectrums Download PDFInfo
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- CN105628679B CN105628679B CN201610167774.2A CN201610167774A CN105628679B CN 105628679 B CN105628679 B CN 105628679B CN 201610167774 A CN201610167774 A CN 201610167774A CN 105628679 B CN105628679 B CN 105628679B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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Abstract
The invention discloses a kind of blood identifiers based on infrared Raman Ultraluminescence super continuous spectrums.Identifier includes power supply module, it can be seen that and infrared spectral coverage spectrometer, Two In and Two Out optical fiber, master control and data analysis system, integrating sphere, test tube manipulator, connecting rod, guide rail, robot movement controller, sample room outer cover plate, bottom plate, stent, light source chamber outer cover plate, fiber optic collimator connector, laser tail optical fiber, super continuous spectrums laser, objective lens support, microcobjective, object lens connector, fiber optic collimator mirror, Y shape coaxial optical fiber, first and second fiber optic connector, Y shape optical fiber, infrared and ultraviolet narrow linewidth laser.Hardware uses Y shape fiberoptic connection Two In and Two Out optical fiber multiplexing spectrometer, is superimposed on software using sectionally weighting, realizes infrared Raman spectrum, Ultraluminescence spectrum and super continuous unrestrained integrated spectral data information fusion.The blood identifier of the present invention is applicable to the automatic identification of whole blood, blood plasma and serum.
Description
Technical field
The present invention relates to a kind of people and animal blood classifying apparatus and method, more particularly to one kind is based on infrared Raman, purple
Outer fluorescence combines the instrument and method that are used to seal the contactless discriminating of test tube blood sample of super continuous unrestrained comprehensive laser spectrum.
Background technology
China takes credit system for customs's inlet and outlet of blood and other kinds biomaterial at present, and to all kinds of
The authenticity of biomaterial but can not be detected directly for various reasons.More particularly to special material as blood sample,
Many times condition is not allowed to for open contact sampling.One side blood sample may be detected operational pollution；
The virulence factor that another aspect blood sample may carry itself can cause occupational exposure to testing staff.In view of the foregoing,
It is very urgent to develop the contactless Fast Detection Technique method of blood sample.
Human blood is similar to animal's whole blood principal component, is mainly made of haemocyte and blood plasma, all takes on a red color, and is visually difficult to
Difference, but people and the pattern and composition of animal haemocyte and blood plasma have technicality, select appropriate method can area
Divide human blood and animal blood.The detection method of classical contact can detect part blood parameters, can be according to this
The blood that a little blood parameters are carried out between different genera differentiates.At present, it is commercial to differentiate that product is all based on greatly using more blood
Flow cytometry need to carry out contacting the parameter for the representative phylogenetic feature that the sampling that declines obtains blood.Non-contacting seal blood reflects
It is not a subject for being rich in challenge, because most of species are sealed in either whole blood or serum, blood in cuvette
The samples such as slurry, in ultraviolet, visible, infrared spectral coverage, the optical characteristics externally reflected is extremely similar, in non-sampled detection
In, it is differentiated by optical means extremely difficult.
First, the blood product encapsulated in test tube may the anti-coagulants containing different components, including heparin, ethylenediamine tetrem
Hydrochlorate (edta salt), citrate, oxalates etc., tube material may be quartz glass or PET plastic etc. in addition, and major part is tried
Pipe also all label.These disturbing factors will seriously affect the optical property of blood so that common optics and spectrographic technique exist
It is upper helpless to seal the discriminating of test tube blood.
For these reasons, exploitation is a kind of seals test tube blood for people and animal, including whole blood, serum, blood plasma it is non-
Contact taxonomic history instrument and correlation method are urgent need to solve the problems.
For the problem, the present invention proposes a kind of continuous with reference to visible to middle infrared excess based on infrared Raman, Ultraluminescence
Unrestrained comprehensive laser spectrum for sealing the instrument and method of the contactless discriminating of test tube blood sample, in infrared Raman and ultraviolet
Using narrow linewidth laser in fluorescence spectrum detection, blood sample in test tube is focused to reference to fiber optic collimator and microcobjective, and
It is received after coaxial optical fiber end face design acquisition blood sample to Raman and fluorescent scattering signal using transmitting；In super continuous unrestrained synthesis
In laser spectrum detection, wide spectrum super continuous spectrums lasing light emitter and the integrating sphere of special designing are used as core instrument hardware structure,
Obtain the unrestrained comprehensive laser spectrum data of different samples.The method combination three of the above laser spectrum detection technique of the present invention, and
Using Y shape fiberoptic connection Two In and Two Out optical fiber multiplexing spectrometer, the fusion of spectroscopic data information is realized.Establish different plant species, difference
The fusion spectra database of test tube, different blood, and based on Principal Component Analysis (principal components
Analysis, abbreviation PCA) calculate these fusion spectroscopic datas obtain principal component analysis shot chart, obtained in shot chart people with
Animal's whole blood, blood plasma, serum cluster areas, using these regions as differentiating that criterion seals blood into pedestrian and animal test tube
The non-contact discriminating of sample.
Invention content
It is continuously overflow with reference to visible to middle infrared excess based on infrared Raman, Ultraluminescence the purpose of the present invention is to provide one kind
The instrument for being used to seal the contactless discriminating of test tube blood sample of comprehensive laser spectrum, can be into pedestrian and animal's whole blood, blood plasma
And the automatic identification of serum, meet the needs of detection quarantine departments are to blood product Rough Inspection.
The technical scheme is that be achieved in, super continuous unrestrained synthesis is combined based on infrared Raman, Ultraluminescence
The hardware system of the sealing blood identifier of laser spectrum is mainly by power supply module, visible spectral coverage spectrometer, infrared spectral coverage spectrum
Instrument, Two In and Two Out optical fiber, master control and data analysis system, integrating sphere, test tube manipulator, connecting rod, guide rail, robot movement control
Device processed, sample room outer cover plate, bottom plate, stent, light source chamber outer cover plate, fiber optic collimator connector, laser tail optical fiber, super continuous spectrums laser
Device, objective lens support, microcobjective, object lens connector, fiber optic collimator mirror, Y shape coaxial optical fiber, the first fiber optic connector, the second light
Fine connector, Y shape optical fiber, infrared narrow linewidth laser and ultraviolet narrow linewidth laser composition.
Wherein, integrating sphere is made of integrating sphere right side and the hemisphere of integrating sphere left side two, they are connect by integrating sphere
Head is coupled, and be fixed on bottom plate.Integrating sphere inner wall coats diffusing reflection coating, the light for being irradiated to inner wall is risen unrestrained
Reflect the effect of even light.Integrating sphere right side is provided with integrating sphere sample well, and sample room outer cover plate is mounted on bottom plate and integrating sphere is right
In half portion, enclosure space sample room is formed, to eliminate the influence of stray light.Circular hole, handling master are provided with above the outer cover plate of sample room
Axis passes through the center of integrating sphere sample well and circular hole, and vertical with bottom plate.Integrating sphere left side is provided with integrating sphere light source hole and product
Bulb separation optical fiber interface.
Y shape coaxial optical fiber is made of laser emitting fiber and signal reception optical fiber, and the two pools an optical fiber, optical fiber
End face is coaxial distribution, and central circular is arranged for laser emitting fiber, and outer concentric circular ring region is signal reception optical fiber
Arrangement, the geometric configuration can the backward Raman scattering of efficient reception infrared laser excitation and the backward fluorescence letters of ultraviolet laser excitation
Number.Y shape coaxial optical fiber coupled with fiber optic collimator mirror can be achieved collimation transmitting and receive, fiber optic collimator mirror by object lens connector with
Microcobjective is connected, and infrared and UV laser beam can be focused to spectrum test point and the back scattering of spectrum test point is believed
It number is collected.Microcobjective is fixedly installed on bottom plate by objective lens support.
Y shape optical fiber there are two input terminal and an output terminal, two input terminals couple respectively infrared narrow linewidth laser with
Ultraviolet narrow linewidth laser, output terminal are connected by the second fiber optic connector and the laser emitting fiber in Y shape coaxial optical fiber.
The ultraviolet laser that the infrared laser that Y shape optical fiber can send out infrared narrow linewidth laser is sent out with ultraviolet narrow linewidth laser is simultaneously
It is pooled in laser emitting fiber.
It can be seen that spectral coverage spectrometer uses same light spectrometer optical fiber interface, Two In and Two Out optical fiber point with infrared spectral coverage spectrometer
For two receiving terminals (i.e. two into) and two output terminals (i.e. scene 2).One receiving terminal couples with integrating sphere optical fiber interface, can receive
Collect from the diffusing reflection light for receiving optical axis；Another receiving terminal is joined with signal Raman reception optical fiber by the first fiber optic connector
It connects, collects Raman and fluorescence signal from signal reception optical fiber；Two output terminals respectively with visible spectral coverage spectrometer and red
Outer spectral coverage spectrometer connection.
The visible and infrared spectral coverage super continuous spectrums pulse laser that super continuous spectrums laser is sent out is transmitted through laser tail optical fiber, so
Super continuous spectrums pulse collimated laser beam is exported after carrying out beam collimation by fiber optic collimator connector afterwards, and product is passed through along transmitting optical axis
Bulb separation light source hole enters integrating sphere.
Fiber optic collimator connector is fixed on by stent on bottom plate, and is passed through light source chamber outer cover plate and joined with integrating sphere left side shell
It connects, enclosure space light source chamber is formed, to eliminate the influence of stray light.
The infrared continuous laser beam that infrared narrow linewidth laser is sent out is sent out through the laser in Y shape optical fiber to Y shape coaxial optical fiber
Optical fiber transmission is penetrated, transmits, collimated through fiber optic collimator mirror, microcobjective along laser beam axis after transmitting from laser emitting fiber arrangement
Infrared induction Raman excitation (the note to spectrum test point can be achieved after focusing：Laser beam axis intersects with handling main shaft, and intersection point is
Spectrum test point), the back scattering Raman signal of spectrum test point is coaxial by Y shape after microcobjective and fiber optic collimator mirror successively
The arrangement of the outer concentric circular ring region of fiber end face, i.e. signal reception optical fiber is collected, then through signal reception optical fiber, Two In and Two Out light
Fibre to infrared spectral coverage spectrometer is received and is analyzed.
The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser is sent out is sent out through the laser in Y shape optical fiber to Y shape coaxial optical fiber
Optical fiber transmission is penetrated, transmits, collimated through fiber optic collimator mirror, microcobjective along laser beam axis after transmitting from laser emitting fiber arrangement
It can be achieved to excite the ultraviolet induced fluorescence of spectrum test point after focusing, the back scattering fluorescence signal of spectrum test point passes through successively
It is arranged after microcobjective and fiber optic collimator mirror by the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. signal reception optical fiber
It collects, then is received and analyzed through signal reception optical fiber, Two In and Two Out optical fiber to visible spectral coverage spectrometer.
Guide rail and bottom plate right angle setting, test tube manipulator are coupled with guide rail by connecting rod and can be controlled in robot movement
It is slided under device control along guide rail.Blood to be checked is encapsulated in test tube by test tube cap.Test tube manipulator is in robot movement controller
Under control, test tube cap can be firmly grasped and test tube is driven to move up and down along handling major axes orientation.
Power supply module is to visible spectral coverage spectrometer, infrared spectral coverage spectrometer, master control and data analysis system, super continuous
Spectrum laser, infrared narrow linewidth laser, ultraviolet narrow linewidth laser and robot movement controller are powered.Master control and number
According to analysis system to visible spectral coverage spectrometer, infrared spectral coverage spectrometer, super continuous spectrums laser, infrared narrow-linewidth laser
Device, ultraviolet narrow linewidth laser and robot movement controller are controlled, and are passed through USB interface and received visible spectral coverage spectrometer
And the spectroscopic data of infrared spectral coverage spectrometer output is handled and is analyzed.Master control and data analysis system included touch screen are man-machine
Interactive interface for the human-computer interaction with user, receives the instruction of user and exports result to user.
The blood point of the sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman, Ultraluminescence
Its step of analysis method is：
(1) instrument starts and super continuous spectrums test sample introduction
Power supply module is opened, to visible spectral coverage spectrometer, infrared spectral coverage spectrometer, master control and data analysis system, super company
Continuous spectrum laser, infrared narrow linewidth laser, ultraviolet narrow linewidth laser and robot movement controller are powered.
User starts test main program by touch screen human-computer interaction interface.At this point, master control and data analysis system are sent out
Sample introduction, which instructs, gives robot movement controller, and robot movement controller control test tube manipulator captures test tube sample introduction to its edge dress
It unloads major axes orientation and enters integrating sphere.Test tube bottom position is higher than transmitting optical axis and non-intersect with receiving optical axis, avoids laser beam straight
Irradiation test tube to be checked is connect, and the transflector light of test tube is avoided directly to transmit along transmitting optical axis and collected by Two In and Two Out optical fiber.
(2) super continuous spectrums laser overflows integrated spectral test
Master control and data analysis system send out instruction and start super continuous spectrums laser, visible spectral coverage spectrometer and infrared spectral coverage
Spectrometer.The super continuous spectrums collimated laser beam of super continuous spectrums laser output enters integrating sphere, laser beam irradiation along transmitting optical axis
To after diffusing reflection coating, reflected light is diffusing reflection, i.e., is transmitted in integrating sphere along all directions, become uniform light.Test tube
After by the super continuous spectrums light irradiation of different directions, test tube (note：Including its material and outer labelling) it is unrestrained anti-with blood to be checked
Penetrate, diffusing transmission, absorption, the light after transmitting will be transmitted along space any direction, encounter the diffusing reflection of diffusing reflection coating to arbitrary side
To on the light transmission in integrating sphere with unrestrained integrated spectral influence.
After being collected along the diffusing reflection light of transmitting optical axis transmission by Two In and Two Out optical fiber, sent respectively to visible spectral coverage spectrometer
And infrared spectral coverage spectrometer carries out opto-electronic conversion and becomes spectroscopic data.It can be seen that the sampled point of spectral coverage spectrometer is N1, sampling number
N1=1300 is taken, the sampled point of infrared spectral coverage spectrometer is N2, and sampling number takes N2=512；It can be seen that spectral coverage spectrometer and infrared
The common N=N1+N2 spectroscopic data of spectral coverage spectrometer output, which is sent through USB interface to master control and data analysis system, to be stored.
(3) Raman fluorescence spectrum test sample introduction
Master control and data analysis system, which send out sample and instruct, gives robot movement controller, the control of robot movement controller
Test tube machinery hand-motion test tube removes integrating sphere and sample room to it along handling major axes orientation, until spectrum test point is located in test tube
Raman fluorescence spectrum test sample introduction is completed in the center of blood to be checked at this time.
(4) infrared laser Raman spectrum and ultraviolet laser fluorometric investigation
Master control and data analysis system send out instruction and start infrared narrow linewidth laser, ultraviolet narrow linewidth laser, visible
Spectral coverage spectrometer and infrared spectral coverage spectrometer.
The collimated post-concentration that focuses on of ultraviolet continuous laser beam that ultraviolet narrow linewidth laser is sent out is at spectrum test point
Blood to be checked, the fluorescence spectrum signal that ultraviolet narrow linewidth continuous laser is induced successively after microcobjective and fiber optic collimator mirror by
The arrangement of the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. signal reception optical fiber is collected, then through signal reception optical fiber, two
Opto-electronic conversion is carried out into scene 2 optical fiber to visible spectral coverage spectrometer and becomes spectroscopic data, and fluorescence spectrum is used and is surveyed with super continuous spectrums
Try the same sampling location of visible spectral coverage and sampling number, i.e. N3=N1.It can be seen that N3 spectroscopic data of spectral coverage spectrometer output
It send to master control and data analysis system 7 and is stored through USB interface 6.
Meanwhile the collimated post-concentration that focuses on of infrared continuous laser beam that infrared narrow linewidth laser is sent out is to spectrum test point
The blood to be checked at place, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced is successively through microcobjective and light
It is collected, then through signal by the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. signal reception optical fiber arrangement after fine collimating mirror
Reception optical fiber, Two In and Two Out optical fiber to infrared spectral coverage spectrometer carry out opto-electronic conversion and become spectroscopic data, and Raman spectrum is used
Sampling location and the sampling number as infrared spectral coverage, i.e. N4=N2 are tested with super continuous spectrums.Infrared spectral coverage spectrometer output
N4 spectroscopic data, which is sent through USB interface to master control and data analysis system, to be stored.
(5) data analysis and fusion
It will be seen that the super continuous spectrums laser of spectral coverage overflows N1 spectroscopic data of integrated spectral and N3 spectrum of fluorescence spectrum
Data are weighted superposition, obtain the fusion spectroscopic data of N1 visible spectral coverages.Wherein the weighted value of fluorescence spectrum is F, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-F.(note：F=0.7 in the present embodiment)
The super continuous spectrums laser of infrared spectral coverage is overflow to N2 spectroscopic data of integrated spectral and N4 spectrum of Raman spectrum
Data are weighted superposition, obtain the fusion spectroscopic data of N2 infrared spectral coverages.Wherein the weighted value of Raman spectrum is L, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-L.(note：L=0.4 in the present embodiment)
The fusion spectroscopic data of N1 visible spectral coverages is formed into N number of spectrum altogether with the spectroscopic data that merges of N2 infrared spectral coverages
Data are used for subsequent analysis.It is calculated based on Principal Component Analysis (principal components analysis, abbreviation PCA)
M principal component numerical value of this N number of spectroscopic data, carries out dimension-reduction treatment, and number of principal components M is taken as 7.
(6) blood differentiates and judges
By M principal component numerical value of blood to be checked, obtain its feature vector in M dimension principal components space, by this feature to
Amount is with M dimension principal components space by the people that this blood identifier obtains and animal's whole blood, blood plasma, serum M dimension principal components space number
It is compared according to the cluster centre feature vector in library, determines the type blood of blood to be checked first according to feature vector similarity,
It is whole blood, blood plasma or serum.Then, then the M principal component numerical value by blood to be checked, with the people under the type blood with it is dynamic
The cluster centre feature vector of the M dimension principal component spatial databases of object different genera is compared, according to feature vector similarity
Kind is determined again, is people or animal blood, is which kind of animal blood if animal blood, so far, completes blood to be checked
Type blood and kind judgement.
Then, master control and data analysis system induce the infrared laser induction Raman spectrum of blood to be checked, ultraviolet laser
Fluorescence spectrum and visible infrared excess continuously overflow the curve of spectrum of comprehensive laser spectrum and judgement result to be shown in touch screen man-machine
On interactive interface, with for reference.So far entire test process is completed.
The invention has the advantages that narrow-linewidth laser is used in infrared Raman spectrum and Ultraluminescence spectral detection
Device focuses to blood sample in test tube, and receive the design of coaxial optical fiber end face using transmitting with reference to fiber optic collimator and microcobjective
To Raman and fluorescent scattering signal after acquisition blood sample, blood laser infrared Raman and Ultraluminescence light in test tube are effectively improved
The signal-to-noise ratio of spectrum signal；Using the integrating sphere of special designing, either blood plasma, the diffusing transmission of serum transparency liquid or whole blood
And the diffusing reflection of test tube label and the unrestrained suction of entire test sample are penetrated and the wide range segment signal of unrestrained transmitting can receive, you can
It is uniformly adopted suitable for the spectroscopic data under all kinds of blood, all kinds of material test tubes, all kinds of different anti-coagulants and different label conditions
Collection.Employ it is visible in infrared high power super continuous spectrums lasing light emitter and corresponding bispectrum section spectrometer, obtain wide spectrum overflow it is comprehensive
Laser spectrum data are closed, the subtle optical difference for encapsulating blood can be captured, discriminating accuracy can be improved；Hardware uses Y
Shape fiberoptic connection Two In and Two Out optical fiber multiplexing spectrometer using weighted superposition on software, realizes the fusion of spectroscopic data information.Due to
Hardware configuration and the robustness of software analysis, blood identifier of the invention are applicable to the automatic knowledge of whole blood, blood plasma and serum
Not.
Description of the drawings
Fig. 1 is the principle of the present invention figure, wherein (a) super continuous spectrums laser overflows integrated spectral test；(b) infrared laser Raman
And ultraviolet laser fluorescence spectrum test；(c) 1 figure of fibre profile；(d) 2 figure of fibre profile；(e) 3 figure of fibre profile.In figure：
1 --- power supply module；2 --- visible spectral coverage spectrometer；3 --- infrared spectral coverage spectrometer；4 --- spectrometer optical fiber interface；
5 --- Two In and Two Out optical fiber；6 --- USB interface；7 --- master control and data analysis system；8 --- integrating sphere optical fiber interface；
9 --- integration spherojoint；10 --- test tube manipulator；11 --- connecting rod；12 --- guide rail；13 --- robot movement controls
Device；14 --- test tube cap；15 --- test tube；16 --- circular hole；17 --- integrating sphere sample well；18 --- sample room outer cover plate；
19 --- blood to be checked；20 --- receive optical axis；21 --- sample room；22 --- bottom plate；23 --- integrating sphere right side；
24 --- diffusing reflection coating；25 --- transmitting optical axis；26 --- integrating sphere left side；27 --- light source chamber；28 --- integrating sphere
Light source hole；29 --- stent；30 --- light source chamber outer cover plate；31 --- fiber optic collimator connector；32 --- laser tail optical fiber；
33 --- touch screen human-computer interaction interface；34 --- super continuous spectrums laser；35 --- integrating sphere；36 --- handling main shaft；
37 --- laser beam axis；38 --- spectrum test point；39 --- objective lens support；40 --- microcobjective；41 --- object lens couple
Device；42 --- fiber optic collimator mirror；43 --- Y shape coaxial optical fiber；44 --- the first fiber optic connector；45 --- Laser emission light
It is fine；46 --- signal reception optical fiber；47 --- signal reception optical fiber arranges；48 --- laser emitting fiber arranges；49 --- it is red
Outer narrow linewidth laser；50 --- the second fiber optic connector；51 --- Y shape optical fiber；52 --- ultraviolet narrow linewidth laser.
Specific embodiment
The hardware system of the sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman, Ultraluminescence
Unite structure it is as shown in Figure 1, hardware system mainly by power supply module 1, visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, two into
Scene 2 optical fiber 5, master control and data analysis system 7, integrating sphere 35, test tube manipulator 10, connecting rod 11, guide rail 12, manipulator fortune
Movement controller 13, sample room outer cover plate 18, bottom plate 22, stent 29, light source chamber outer cover plate 30, fiber optic collimator connector 31, laser
Tail optical fiber 32, super continuous spectrums laser 34, objective lens support 39, microcobjective 40, object lens connector 41, fiber optic collimator mirror 42, Y shape are same
It is axis optical fiber 43, the first fiber optic connector 44, the second fiber optic connector 50, Y shape optical fiber 51, infrared narrow linewidth laser 49, ultraviolet
Narrow linewidth laser 52 forms.
Wherein, integrating sphere 35 is made of integrating sphere right side 23 and the hemisphere of integrating sphere left side 26 two, they pass through product
Bulb separation connector 9 is coupled, and is fixed on bottom plate 22.35 inner wall of integrating sphere coating diffusing reflection coating 24, to being irradiated to
The light of inner wall plays the even light of diffusing reflection.Integrating sphere right side 23 is provided with integrating sphere sample well 17, sample room outer cover plate 18
On bottom plate 22 and integrating sphere right side 23, enclosure space sample room 21 is formed, to eliminate the influence of stray light.Sample room
The top of outer cover plate 18 is provided with circular hole 16, and handling main shaft 36 passes through the center of integrating sphere sample well 17 and circular hole 16, and with bottom plate 22
Vertically.Integrating sphere left side 26 is provided with integrating sphere light source hole 28 and the (note of integrating sphere optical fiber interface 8：It is SMA905 in the present embodiment
Interface).
Y shape coaxial optical fiber 43 is made of laser emitting fiber 45 and signal reception optical fiber 46, and the two pools an optical fiber,
Its fiber end face is coaxial distribution, and central circular arranges 48 for laser emitting fiber, and outer concentric circular ring region is signal
Reception optical fiber arrangement 47, the geometric configuration can efficient reception infrared laser excitation backward Raman scattering signal and ultraviolet laser
The backward fluorescence signal of excitation.Y shape coaxial optical fiber 43 is coupled with fiber optic collimator mirror 42 can be achieved collimation transmitting and receives, and optical fiber is accurate
Straight mirror 42 is connected by object lens connector 41 and microcobjective 40, and infrared and UV laser beam can be focused to spectrum test point
38 and the backscatter signal of spectrum test point 38 is collected.Microcobjective 40 is fixedly installed in bottom plate by objective lens support 39
On 22.
For Y shape optical fiber 51 there are two input terminal and an output terminal, two input terminals to couple infrared narrow linewidth laser respectively
49 pass through the second fiber optic connector 50 and the Laser emission in Y shape coaxial optical fiber 43 with ultraviolet narrow linewidth laser 52, output terminal
Optical fiber 45 is connected.The infrared laser that Y shape optical fiber 51 can send out infrared narrow linewidth laser 49 and ultraviolet narrow linewidth laser
52 ultraviolet lasers sent out are pooled to simultaneously in laser emitting fiber 45.
It can be seen that spectral coverage spectrometer 2 (is noted with infrared spectral coverage spectrometer 3 using same light spectrometer optical fiber interface 4：The present embodiment
In be SMA905 interfaces), Two In and Two Out optical fiber 5 is divided to for two receiving terminals (i.e. two into) and two output terminals (i.e. scene 2).One
Receiving terminal couples with integrating sphere optical fiber interface 8, collects from the diffusing reflection light for receiving optical axis 20；Another receiving terminal and letter
Number reception optical fiber 46 couples (note by the first fiber optic connector 44：It is SMA905 connectors in the present embodiment), it collects and comes from
The Raman and fluorescence signal of signal reception optical fiber 46；Two output terminals respectively with visible spectral coverage spectrometer 2 and infrared spectral coverage spectrum
Instrument 3 couples.
(the note of super continuous spectrums laser 34：Its spectral region 400nm-2400nm in the present embodiment, power 1W, repetition 1MHz,
Pulsewidth 150ps) the visible and infrared spectral coverage super continuous spectrums pulse laser that sends out transmits through laser tail optical fiber 32, then pass through optical fiber
It collimates after connector 31 carries out beam collimation and exports super continuous spectrums pulse collimated laser beam, and integrating sphere light is passed through along transmitting optical axis 25
Source aperture 28 enters integrating sphere 35.
Fiber optic collimator connector 31 is fixed on by stent 29 on bottom plate 22, and passes through light source chamber outer cover plate 30 and integrating sphere left half
Portion's 26 shell connection, forms enclosure space light source chamber 27, to eliminate the influence of stray light.
The infrared continuous laser beam that infrared narrow linewidth laser 49 is sent out is through in Y shape optical fiber 51 to Y shape coaxial optical fiber 43
Laser emitting fiber 45 is transmitted, is transmitted, through fiber optic collimator mirror along laser beam axis 37 after transmitting from laser emitting fiber arrangement 48
Infrared induction Raman excitation (the note to spectrum test point 38 can be achieved in 42 collimations, microcobjective 40 after focusing on：Laser beam axis 37 with
It is intersecting to load and unload main shaft 36, intersection point is spectrum test point 38), the back scattering Raman signal of spectrum test point 38 is successively through micro-
It is arranged after object lens 40 and fiber optic collimator mirror 42 by the outer concentric circular ring region of 43 end face of Y shape coaxial optical fiber, i.e. signal reception optical fiber
Row 47 are collected, then are received and analyzed through signal reception optical fiber 46, Two In and Two Out optical fiber 5 to infrared spectral coverage spectrometer 3.
The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser 52 is sent out is through in Y shape optical fiber 51 to Y shape coaxial optical fiber 43
Laser emitting fiber 45 is transmitted, is transmitted, through fiber optic collimator mirror along laser beam axis 37 after transmitting from laser emitting fiber arrangement 48
42 collimations, microcobjective 40 can be achieved to excite the ultraviolet induced fluorescence of spectrum test point 38 after focusing on, spectrum test point 38
Outer ring of the back scattering fluorescence signal successively by 43 end face of Y shape coaxial optical fiber after microcobjective 40 and fiber optic collimator mirror 42 is concentric
Circle ring area, i.e. signal reception optical fiber arrangement 47 is collected, then through signal reception optical fiber 46, Two In and Two Out optical fiber 5 to visible spectral coverage
Spectrometer 2 is received and is analyzed.
Guide rail 12 and 22 right angle setting of bottom plate, test tube manipulator 10 are coupled with guide rail 12 by connecting rod 11 and can be in machineries
Hands movement controller 13 slides under controlling along guide rail 12.Blood 19 to be checked is encapsulated in by test tube cap 14 in test tube 15.Test tube machinery
Hand 10 can firmly grasp test tube cap 14 and drive test tube 15 along above and below handling main shaft 36 direction under the control of robot movement controller 13
Movement.
Power supply module 1 is to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, master control and data analysis system 7, super
Continuous spectrum laser 34, infrared narrow linewidth laser 49, ultraviolet narrow linewidth laser 52 and robot movement controller 13 carry out
Power supply.Master control and data analysis system 7 are to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, super continuous spectrums laser
34th, infrared narrow linewidth laser 49, ultraviolet narrow linewidth laser 52 and robot movement controller 13 are controlled, and pass through
USB interface 6 receives visible spectral coverage spectrometer 2 and the spectroscopic data of infrared spectral coverage spectrometer 3 output is handled and analyzed.Master control
And 7 included touch screen human-computer interaction interface 33 of data analysis system, for the human-computer interaction with user, receive the instruction of user simultaneously
Result is exported to user.
The blood point of the sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman, Ultraluminescence
Its step of analysis method is：
(1) instrument starts and super continuous spectrums test sample introduction
Open power supply module 1, to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, master control and data analysis system 7,
Super continuous spectrums laser 34, infrared narrow linewidth laser 49, ultraviolet narrow linewidth laser 52 and robot movement controller 13 into
Row power supply.
User starts test main program by touch screen human-computer interaction interface 33.At this point, master control and data analysis system 7 are sent out
Go out sample introduction to instruct to robot movement controller 13, robot movement controller 13 control test tube manipulator 10 capture test tube 15 into
Sample enters integrating sphere 35 to it along 36 direction of handling main shaft.15 bottom position of test tube is higher than transmitting optical axis 25 and with receiving optical axis 20
It is non-intersect, laser beam direct irradiation test tube 15 to be checked is avoided, and avoid the transflector light of test tube 15 directly along transmitting optical axis 25
It transmits and is collected by Two In and Two Out optical fiber 5.
(2) super continuous spectrums laser overflows integrated spectral test
As shown in Figure 1a, master control and data analysis system 7 send out instruction startup super continuous spectrums laser 34, visible spectral coverage light
Spectrometer 2 and infrared spectral coverage spectrometer 3.Super continuous spectrums laser 34 export super continuous spectrums collimated laser beam along transmitting optical axis 25 into
Enter integrating sphere 35, after laser beam is irradiated to diffusing reflection coating 24, reflected light is diffusing reflection, i.e., along all directions in integrating sphere 35
Transmission, becomes uniform light.Test tube 15 is after by the super continuous spectrums light irradiation of different directions, (the note of test tube 15：Including its material
With outer labelling) it will be transmitted with the light after the diffusing reflection of blood 19 to be checked, diffusing transmission, absorption, transmitting along space any direction,
24 diffusing reflection of diffusing reflection coating is encountered to any direction, there is unrestrained integrated spectral to influence the light transmission in integrating sphere 35.
After the diffusing reflection light transmitted along transmitting optical axis 25 is collected by Two In and Two Out optical fiber 5, sent respectively to visible spectral coverage light
Spectrometer 2 and infrared spectral coverage spectrometer 3 carry out opto-electronic conversion and become spectroscopic data.Visible spectral coverage spectrometer 2 in this specific embodiment
Spectral region be 370-750nm, sampled point N1=1300.The spectral region of infrared spectral coverage spectrometer 3 is 800-1750nm,
Sampled point is N2=512.It can be seen that spectral coverage spectrometer 2 and the common N=N1+N2 spectroscopic data warp of infrared spectral coverage spectrometer 3 output
USB interface 6 send to master control and data analysis system 7 and is stored.
(3) Raman fluorescence spectrum test sample introduction
Master control and data analysis system 7 send out sample and instruct to robot movement controller 13, robot movement controller
13 control test tube manipulators 10 drive test tube 15 to remove integrating sphere 35 and sample room 21 to it along 36 direction of handling main shaft, until spectrum
Test point 38 is located at the center of blood 19 to be checked in test tube 15, completes Raman fluorescence spectrum test sample introduction at this time.
(4) infrared laser Raman and the test of ultraviolet laser fluorescence spectrum
As shown in Figure 1 b, master control and data analysis system 7 send out the infrared narrow linewidth laser 49 of instruction startup, ultraviolet narrow line
Wide laser 52, visible spectral coverage spectrometer 2 and infrared spectral coverage spectrometer 3.
Ultraviolet (the note of narrow linewidth laser 52：Wavelength is used in the present embodiment, and for 360nm ± 1nm, power 0.05W's partly leads
Body pumped solid continuous wave laser) the collimated post-concentration that focuses on of the ultraviolet continuous laser beam that sends out treats at spectrum test point 38
Blood 19 is examined, the fluorescence spectrum signal that ultraviolet narrow linewidth continuous laser is induced is successively through microcobjective 40 and fiber optic collimator mirror 42
Afterwards by the outer concentric circular ring region of 43 end face of Y shape coaxial optical fiber, i.e. signal reception optical fiber arrangement 47 is collected, then received through signal
Optical fiber 46, Two In and Two Out optical fiber 5 to visible spectral coverage spectrometer 2 carry out opto-electronic conversion and become spectroscopic data, in this specific embodiment
It can be seen that the spectral region of spectral coverage spectrometer 2 is 370-750nm, fluorescence spectrum is used and is tested as visible spectral coverage with super continuous spectrums
Sampling location and sampling number, i.e. N3=N1=1300.Since the wavelength of ultraviolet narrow linewidth laser 52 is located at visible spectral coverage
Except the spectral region of spectrometer 2, therefore ultraviolet laser echo does not interfere with the acquisition to fluorescence spectrum signal, without auspicious
Sharp optical filter inhibits echo interference.It can be seen that the N3 spectroscopic data that spectral coverage spectrometer 2 exports is sent through USB interface 6 to master control and number
It is stored according to analysis system 7.
Meanwhile infrared (the note of narrow linewidth laser 49：Wavelength is used in the present embodiment as 785nm ± 1nm, power 0.3W's
Semiconductor pumped solid continuous wave laser) the collimated post-concentration that focuses on of the infrared continuous laser beam that sends out is at spectrum test point 38
Blood to be checked 19, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced successively through microcobjective 40 and
It is collected after fiber optic collimator mirror 42 by the outer concentric circular ring region of 43 end face of Y shape coaxial optical fiber, i.e. signal reception optical fiber arrangement 47,
Opto-electronic conversion is carried out through signal reception optical fiber 46, Two In and Two Out optical fiber 5 to infrared spectral coverage spectrometer 3 become spectroscopic data again, this
The spectral region of infrared spectral coverage spectrometer 3 is 800-1750nm in specific embodiment, and Raman spectrum is used and is surveyed with super continuous spectrums
Try the same sampling location of infrared spectral coverage and sampling number, i.e. N4=N2=512.Due to the wavelength of infrared narrow linewidth laser 49
Except the spectral region of infrared spectral coverage spectrometer 3, therefore infrared laser echo does not interfere with and raman spectral signal is adopted
Collection inhibits echo interference without Rayleigh optical filter.The N4 spectroscopic data that infrared spectral coverage spectrometer 3 exports is through USB interface 6
It send to master control and data analysis system 7 and is stored.
(5) data analysis and fusion
It will be seen that the super continuous spectrums laser of spectral coverage overflows N1 spectroscopic data of integrated spectral and N3 spectrum of fluorescence spectrum
Data are weighted superposition, obtain the fusion spectroscopic data of N1 visible spectral coverages.Wherein the weighted value of fluorescence spectrum is F, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-F.(note：F=0.7 in the present embodiment)
The super continuous spectrums laser of infrared spectral coverage is overflow to N2 spectroscopic data of integrated spectral and N4 spectrum of Raman spectrum
Data are weighted superposition, obtain the fusion spectroscopic data of N2 infrared spectral coverages.Wherein the weighted value of Raman spectrum is L, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-L.(note：L=0.4 in the present embodiment)
The fusion spectroscopic data of N1 visible spectral coverages is formed into N number of spectrum altogether with the spectroscopic data that merges of N2 infrared spectral coverages
Data are used for subsequent analysis.It is calculated based on Principal Component Analysis (principal components analysis, abbreviation PCA)
The M principal component numerical value (note of this N number of spectroscopic data：M=7 in the present embodiment calculates 7 principal component numerical value), it is dropped
Dimension processing.
(6) blood differentiates and judges
By M principal component numerical value of blood 19 to be checked, its feature vector in M dimension principal components space is obtained, by this feature
Vector is with M dimension principal components space by the people that this blood identifier obtains and animal's whole blood, blood plasma, serum M dimension principal components space
The cluster centre feature vector of database is compared, and determines the blood class of blood 19 to be checked first according to feature vector similarity
Type is whole blood, blood plasma or serum.Then, then the M principal component numerical value by blood 19 to be checked, under the type blood
People and the cluster centre feature vector of the M dimension principal component spatial databases of animal different genera are compared, according to feature vector
Similarity determines kind again, is people or animal blood, is which kind of animal blood if animal blood, so far, completes to treat
Examine the type blood of blood 19 and the judgement of kind.
Then, master control and data analysis system 7 lure the infrared laser induction Raman spectrum of blood 19 to be checked, ultraviolet laser
Fluorescence spectrum and visible infrared excess is led continuously to overflow the curve of spectrum of comprehensive laser spectrum and judge that result is shown in touch screen people
On machine interactive interface 33, with for reference.So far entire test process is completed.
Claims (1)
1. a kind of sealing blood that super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman, Ultraluminescence differentiates instrument, it
Including power supply module (1), visible spectral coverage spectrometer (2), infrared spectral coverage spectrometer (3), Two In and Two Out optical fiber (5), master control and number
According to analysis system (7), integrating sphere (35), test tube manipulator (10), connecting rod (11), guide rail (12), robot movement controller
(13), sample room outer cover plate (18), bottom plate (22), stent (29), light source chamber outer cover plate (30), fiber optic collimator connector (31), swash
Light device tail optical fiber (32), super continuous spectrums laser (34), objective lens support (39), microcobjective (40), object lens connector (41), optical fiber
Collimating mirror (42), Y shape coaxial optical fiber (43), the first fiber optic connector (44), the second fiber optic connector (50), Y shape optical fiber (51),
Infrared narrow linewidth laser (49), ultraviolet narrow linewidth laser (52), it is characterised in that：
The integrating sphere (35) is made of integrating sphere right side (23) and the hemisphere of integrating sphere left side (26) two, they pass through
Integration spherojoint (9) is coupled, and be fixed on bottom plate (22)；Integrating sphere (35) inner wall coats diffusing reflection coating
(24), play the even light of diffusing reflection to the light for being irradiated to inner wall；Integrating sphere right side (23) is provided with integrating sphere sample well
(17), sample room outer cover plate (18) forms enclosure space sample room on bottom plate (22) and integrating sphere right side (23)
(21), to eliminate the influence of stray light；Circular hole (16) is provided with above sample room outer cover plate (18), loads and unloads main shaft (36) across integration
The center of ball sample well (17) and circular hole (16), and it is vertical with bottom plate (22)；Integrating sphere left side (26) is provided with integrating sphere light source
Hole (28) and integrating sphere optical fiber interface (8)；
The Y shape coaxial optical fiber (43) is made of laser emitting fiber (45) and signal reception optical fiber (46), and the two pools
A piece optical fiber, fiber end face are coaxial distribution, and central circular arranges (48) for laser emitting fiber, outer concentric circular ring
Region for signal reception optical fiber arrange (47), the geometric configuration can efficient reception infrared laser excitation backward Raman scattering signal
And the backward fluorescence signal of ultraviolet laser excitation；Y shape coaxial optical fiber (43) is coupled with fiber optic collimator mirror (42) can be achieved collimation
It transmitting and receives, fiber optic collimator mirror (42) is connected by object lens connector (41) and microcobjective (40), can will be infrared and purple
Outer laser beam focus is collected to spectrum test point (38) and to the backscatter signal of spectrum test point (38)；Objective lens support
(39) microcobjective (40) is fixedly installed on bottom plate (22)；
There are two input terminal and an output terminal, two input terminals to couple infrared narrow linewidth and swash the Y shape optical fiber (51) respectively
Light device (49) and ultraviolet narrow linewidth laser (52), output terminal pass through the second fiber optic connector (50) and Y shape coaxial optical fiber (43)
In laser emitting fiber (45) be connected；The infrared laser that Y shape optical fiber (51) can send out infrared narrow linewidth laser (49)
The ultraviolet laser sent out with ultraviolet narrow linewidth laser (52) is pooled to simultaneously in laser emitting fiber (45)；
The visible spectral coverage spectrometer (2) and infrared spectral coverage spectrometer (3) are using same light spectrometer optical fiber interface (4), and two
It is divided into two receiving terminals and two output terminals into scene 2 optical fiber (5)；One receiving terminal couples with integrating sphere optical fiber interface (8), can
It collects from the diffusing reflection light for receiving optical axis (20)；Another receiving terminal is joined with signal reception optical fiber (46) by the first optical fiber
Device (44) connection is connect, collects Raman and fluorescence signal from signal reception optical fiber (46)；Two output terminals respectively with it is visible
Spectral coverage spectrometer (2) and infrared spectral coverage spectrometer (3) connection；
The visible and infrared spectral coverage super continuous spectrums pulse laser that the super continuous spectrums laser (34) is sent out is through laser tail optical fiber
(32) it transmits, super continuous spectrums pulse collimated laser beam is exported after then carrying out beam collimation by fiber optic collimator connector (31), and
Along transmitting optical axis (25) across integrating sphere light source hole (28) into integrating sphere (35)；
The fiber optic collimator connector (31) is fixed on by stent (29) on bottom plate (22), and pass through light source chamber outer cover plate (30) with
Integrating sphere left side (26) shell couples, and enclosure space light source chamber (27) is formed, to eliminate the influence of stray light；
The infrared continuous laser beam that the infrared narrow linewidth laser (49) is sent out is through Y shape optical fiber (51) to Y shape coaxial optical fiber
(43) laser emitting fiber (45) in is transmitted, is passed after transmitting along laser beam axis (37) from laser emitting fiber arrangement (48)
Defeated, the achievable infrared induction drawing to spectrum test point (38) after fiber optic collimator mirror (42) collimation, microcobjective (40) focus on
Graceful excitation, the back scattering Raman signal of spectrum test point (38) successively after microcobjective (40) and fiber optic collimator mirror (42) by
The outer concentric circular ring region of Y shape coaxial optical fiber (43) end face, i.e. signal reception optical fiber arrangement (47) are collected, then are received through signal
Optical fiber (46), Two In and Two Out optical fiber (5) to infrared spectral coverage spectrometer (3) are received and are analyzed；
The ultraviolet continuous laser beam that the ultraviolet narrow linewidth laser (52) is sent out is through Y shape optical fiber (51) to Y shape coaxial optical fiber
(43) laser emitting fiber (45) in is transmitted, is passed after transmitting along laser beam axis (37) from laser emitting fiber arrangement (48)
It is defeated, achievable glimmering to the uv induction of spectrum test point (38) after fiber optic collimator mirror (42) collimation, microcobjective (40) focus on
Light excites, the back scattering fluorescence signal of spectrum test point (38) successively after microcobjective (40) and fiber optic collimator mirror (42) by
The outer concentric circular ring region of Y shape coaxial optical fiber (43) end face, i.e. signal reception optical fiber arrangement (47) are collected, then are received through signal
Optical fiber (46), Two In and Two Out optical fiber (5) to visible spectral coverage spectrometer (2) are received and are analyzed；
The guide rail (12) and bottom plate (22) right angle setting, test tube manipulator (10) are joined by connecting rod (11) and guide rail (12)
It connects and can be slided under robot movement controller (13) control along guide rail (12)；Blood (19) to be checked is encapsulated by test tube cap (14)
In test tube (15)；Test tube manipulator (10) can firmly grasp test tube cap (14) and band under robot movement controller (13) control
Dynamic test tube (15) moves up and down along handling main shaft (36) direction；
The power supply module (1) is to visible spectral coverage spectrometer (2), infrared spectral coverage spectrometer (3), master control and data analysis
System (7), super continuous spectrums laser (34), infrared narrow linewidth laser (49), ultraviolet narrow linewidth laser (52) and manipulator
Motion controller (13) is powered；Master control and data analysis system (7) are to visible spectral coverage spectrometer (2), infrared spectral coverage
Spectrometer (3), super continuous spectrums laser (34), infrared narrow linewidth laser (49), ultraviolet narrow linewidth laser (52) and machinery
Hands movement controller (13) is controlled, and is passed through USB interface (6) and received visible spectral coverage spectrometer (2) and infrared spectral coverage spectrum
The spectroscopic data of instrument (3) output is handled and is analyzed；Master control and data analysis system (7) included touch screen human-computer interaction interface
(33), for the human-computer interaction with user, receive the instruction of user and export result to user；
Master control and data analysis system send out instruction and start super continuous spectrums laser, visible spectral coverage spectrometer and infrared spectral coverage spectrum
Instrument, the super continuous spectrums collimated laser beam of super continuous spectrums laser output enter integrating sphere along transmitting optical axis, and laser beam is irradiated to unrestrained
After reflectance coating, reflected light is diffusing reflection, after test tube is irradiated by the super continuous spectrums light of different directions, test tube, test tube outer patch
Light after the diffusing reflection of label and blood to be checked, diffusing transmission, absorption, transmitting will transmit along space any direction, encounter it is unrestrained instead
Coating diffusing reflection is penetrated to any direction, there is unrestrained integrated spectral to influence the light transmission in integrating sphere；It is passed along transmitting optical axis
After defeated diffusing reflection light is collected by Two In and Two Out optical fiber, sent respectively to visible spectral coverage spectrometer and the progress of infrared spectral coverage spectrometer
Opto-electronic conversion becomes spectroscopic data；Master control and data analysis system send out instruction and start infrared narrow linewidth laser, ultraviolet narrow line
Wide laser, visible spectral coverage spectrometer and infrared spectral coverage spectrometer；The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser is sent out
The collimated blood to be checked for focusing on post-concentration at spectrum test point, the fluorescence spectrum letter that ultraviolet narrow linewidth continuous laser is induced
It is received after microcobjective and fiber optic collimator mirror by the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. signal number successively
Optical fiber arrangement is collected, then is carried out opto-electronic conversion through signal reception optical fiber, Two In and Two Out optical fiber to visible spectral coverage spectrometer and become light
Modal data；The collimated post-concentration that focuses on of infrared continuous laser beam that infrared narrow linewidth laser is sent out is treated at spectrum test point
Blood is examined, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced is successively through microcobjective and fiber optic collimator
By the outer concentric circular ring region of Y shape coaxial optical fiber end face after mirror, i.e. signal reception optical fiber arrangement is collected, then receives light through signal
Fine, Two In and Two Out optical fiber to infrared spectral coverage spectrometer carries out opto-electronic conversion and becomes spectroscopic data；It can be seen that spectral coverage spectrometer and infrared
The spectroscopic data of spectral coverage spectrometer output, which is sent through USB interface to master control and data analysis system, to be stored, is analyzed and processed.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108267571A (en) * | 2017-01-03 | 2018-07-10 | 中国医学科学院医学实验动物研究所 | A kind of blood kind sentences method for distinguishing |
| CN107064084B (en) * | 2017-03-13 | 2023-10-17 | 哈尔滨工业大学（威海） | Microminiature laser fluorescence spectrometer and spectrum detection method |
| CN107643253B (en) * | 2017-10-13 | 2020-01-10 | 中国科学院上海技术物理研究所 | Cryopreserved whole blood analyzer based on time domain resolution supercontinuum |
| CN108303422B (en) * | 2018-01-26 | 2020-09-15 | 中国科学院广州生物医药与健康研究院 | Imaging device |
| CN111323399A (en) * | 2018-12-15 | 2020-06-23 | 中国科学院深圳先进技术研究院 | Multi-color fluorescence synchronous detection liquid drop micro-fluidic chip |
| CN112558230B (en) * | 2020-12-07 | 2022-10-21 | 中国科学院合肥物质科学研究院 | Multichannel time-sharing retest optical coupling device and use method thereof |
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