CN105866095B - Blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman - Google Patents
Blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman Download PDFInfo
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- CN105866095B CN105866095B CN201610167545.0A CN201610167545A CN105866095B CN 105866095 B CN105866095 B CN 105866095B CN 201610167545 A CN201610167545 A CN 201610167545A CN 105866095 B CN105866095 B CN 105866095B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
Abstract
The invention discloses a kind of blood identifiers based on the super continuous unrestrained comprehensive spectrum of infrared Raman.Power supply module is being powered visible and infrared spectral coverage spectrometer, master control and data analysis system, super continuous spectrums and infrared narrow linewidth laser and robot movement controller.Master control and data analysis system receive the spectroscopic data that visible spectral coverage spectrometer and infrared spectral coverage spectrometer export by USB interface and are handled and analyzed to control visible spectral coverage spectrometer, infrared spectral coverage spectrometer, super continuous spectrums laser, infrared narrow linewidth laser and robot movement controller.The invention has the advantages that hardware uses Y shape fiberoptic connection Two In and Two Out optical fiber multiplexing spectrometer, infrared Raman spectrum and super continuous unrestrained comprehensive laser spectrum weighted superposition are used on software, realizes that spectroscopic data information merges.The automatic identification that the robustness that hardware configuration and software are analyzed makes this blood identifier be applicable to whole blood, blood plasma and serum.
Description
Technical field
The present invention relates to a kind of people and animal blood classifying apparatus and method, more particularly to a kind of infrared Raman that is based on to combine
The instrument and method for sealing the contactless discriminating of test tube blood sample of super continuous unrestrained comprehensive laser spectrum.
Background technology
China takes credit system for customs's inlet and outlet of blood and other kinds biomaterial at present, and to all kinds of
The authenticity of biomaterial but can not be detected directly for various reasons.More particularly to special material as blood sample,
Many times condition is not allowed to for open contact sampling.One side blood sample may be detected operational pollution；
The virulence factor that another aspect blood sample may carry itself can cause occupational exposure to testing staff.In view of the foregoing,
It is very urgent to develop the contactless Fast Detection Technique method of blood sample.
Human blood is similar to animal's whole blood principal component, is mainly made of haemocyte and blood plasma, all takes on a red color, and is visually difficult to
Difference, but people and the pattern and composition of animal haemocyte and blood plasma have technicality, select appropriate method can area
Divide human blood and animal blood.The detection method of classical contact can detect part blood parameters, can be according to this
A little blood parameters carry out the blood between different genera and differentiate.Currently, commercial differentiate that product is all based on greatly using more blood
Flow cytometry need to carry out the parameter for contacting the representative phylogenetic feature that the sampling that declines obtains blood.Non-contacting seal blood reflects
It is not a project for being rich in challenge, because most of species are sealed in either whole blood or serum, blood in cuvette
The samples such as slurry, in ultraviolet, visible, infrared spectral coverage, the optical characteristics externally reflected is extremely similar, in non-sampled detection
In, it is differentiated by optical means extremely difficult.
First, the blood product encapsulated in test tube may contain the anti-coagulants of different components, including heparin, ethylenediamine tetrem
Hydrochlorate (edta salt), citrate, oxalates etc., tube material may be quartz glass or PET plastic etc., most of examination in addition
Pipe also all label.These disturbing factors will seriously affect the optical property of blood so that common optics and spectrographic technique exist
It is upper helpless to seal the discriminating of test tube blood.
For these reasons, exploitation is a kind of sealing test tube blood for people and animal, including whole blood, serum, blood plasma is non-
Contact taxonomic history instrument and correlation method are urgent need to solve the problems.
For the problem, the present invention proposes that a kind of infrared Raman that is based on combines visible to middle infrared excess continuously to overflow comprehensive laser
The instrument and method for sealing the contactless discriminating of test tube blood sample of spectrum, using red in infrared Raman spectral detection
Outer narrow linewidth Ramar laser is focused to blood sample in test tube in conjunction with fiber optic collimator and microcobjective, and is received using transmitting
Coaxial optical fiber end face design acquisition blood sample backward Raman scattering signal；In super continuous unrestrained comprehensive laser spectrum detection, adopt
Integrating sphere with wide spectrum super continuous spectrums lasing light emitter and special designing is core instrument hardware structure, obtains the unrestrained comprehensive of different samples
Close laser spectrum data.Method the combination both the above laser spectrum detection technique of the present invention, and use Y shape fiberoptic connection two into
Scene 2 optical fiber multiplexing spectrometer realizes the fusion of spectroscopic data information.Establish the fusion of different plant species, different test tubes, different blood
Spectra database, and these are calculated based on Principal Component Analysis (principal components analysis, abbreviation PCA)
Fusion spectroscopic data obtains principal component analysis shot chart, and the cluster area of people and animal's whole blood, blood plasma, serum are obtained in shot chart
Domain seals the non-contact discriminating of blood sample using these regions as discriminating criterion into pedestrian and animal test tube.
Invention content
The purpose of the present invention is to provide one kind to combine visible to middle infrared excess continuously to overflow comprehensive laser based on infrared Raman
The instrument for sealing the contactless discriminating of test tube blood sample of spectrum, can be into pedestrian and animal's whole blood, blood plasma and serum
Automatic identification meets the needs of detection quarantine departments are to blood product Rough Inspection.
The technical scheme is that be achieved in, super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman
The hardware system of blood identifier is sealed mainly by power supply module, visible spectral coverage spectrometer, infrared spectral coverage spectrometer, Two In and Two Out
Optical fiber, master control and data analysis system, integrating sphere, test tube manipulator, connecting rod, guide rail, robot movement controller, sample room
Outer cover plate, bottom plate, holder, light source chamber outer cover plate, fiber optic collimator connector, laser tail optical fiber, super continuous spectrums laser, object lens branch
Frame, microcobjective, object lens connector, fiber optic collimator mirror, Y shape coaxial optical fiber, fiber optic connector, infrared narrow linewidth laser group
At.
Wherein, integrating sphere is made of integrating sphere right side and the hemisphere of integrating sphere left side two, they are connect by integrating sphere
Head is coupled, and is fixed on bottom plate.Integrating sphere inner wall coats diffusing reflection coating, and the light to being irradiated to inner wall rises unrestrained
Reflect the effect of even light.Integrating sphere right side is provided with integrating sphere sample well, and sample room outer cover plate is mounted on bottom plate and integrating sphere is right
In half portion, enclosure space sample room is formed, to eliminate the influence of stray light.Circular hole, handling master are provided with above the outer cover plate of sample room
Axis passes through the center of integrating sphere sample well and circular hole, and vertical with bottom plate.Integrating sphere left side is provided with integrating sphere light source hole and product
Bulb separation optical fiber interface.
Y shape coaxial optical fiber is made of infrared emission optical fiber and Raman reception optical fiber, and the two pools an optical fiber, optical fiber
End face is coaxial distribution, and central circular arranges for infrared emission optical fiber, and outer concentric circular ring region is Raman reception optical fiber
Arrangement, the geometric configuration can efficient reception infrared laser excitation backward Raman scattering signal.Y shape coaxial optical fiber and fiber optic collimator
Mirror coupling can be achieved collimation transmitting and receive, and fiber optic collimator mirror is connected by object lens connector with microcobjective, can will be infrared
Laser beam focus is collected to Raman spectrum test point and to the backscatter signal of Raman spectrum test point.Objective lens support will
Microcobjective is fixedly installed on bottom plate.
It can be seen that spectral coverage spectrometer is all made of same light spectrometer optical fiber interface, Two In and Two Out optical fiber point with infrared spectral coverage spectrometer
For two receiving terminals (i.e. two into) and two output ends (i.e. scene 2).One receiving terminal couples with integrating sphere optical fiber interface, can receive
Collect from the diffusing reflection light for receiving optical axis；Another receiving terminal is coupled with Raman reception optical fiber by fiber optic connector, can be received
Collect the Raman signal from Raman reception optical fiber；Two output ends join with visible spectral coverage spectrometer and infrared spectral coverage spectrometer respectively
It connects.
The visible and infrared spectral coverage super continuous spectrums pulse laser that super continuous spectrums laser is sent out is transmitted through laser tail optical fiber, so
Super continuous spectrums pulse collimated laser beam is exported after carrying out beam collimation by fiber optic collimator connector afterwards, and product is passed through along transmitting optical axis
Bulb separation light source hole enters integrating sphere.
Fiber optic collimator connector is fixed on by holder on bottom plate, and is joined by light source chamber outer cover plate and integrating sphere left side shell
It connects, enclosure space light source chamber is formed, to eliminate the influence of stray light.
The infrared continuous laser beam that infrared narrow linewidth laser is sent out is passed through the infrared emission optical fiber in Y shape coaxial optical fiber
It is defeated, from infrared emission optical fiber arrangement in emit after along infrared laser optical axis transmission, through fiber optic collimator mirror collimation, microcobjective focus
Infrared induction Raman excitation (the note to Raman spectrum test point can be achieved afterwards：Infrared laser optical axis intersects with handling main shaft, hands over
Point is Raman spectrum test point), the back scattering Raman signal of Raman spectrum test point is successively through microcobjective and fiber optic collimator
By the outer concentric circular ring region of Y shape coaxial optical fiber end face after mirror, i.e. Raman reception optical fiber arrangement is collected, then receives light through Raman
Fine, Two In and Two Out optical fiber to infrared spectral coverage spectrometer is received and is analyzed.
Guide rail and bottom plate right angle setting, test tube manipulator are coupled with guide rail by connecting rod and can be controlled in robot movement
It is slided along guide rail under device control.Blood to be checked is encapsulated in test tube by test tube cap.Test tube manipulator is in robot movement controller
Under control, test tube cap can be firmly grasped and test tube is driven to move up and down along handling major axes orientation.
Power supply module is to visible spectral coverage spectrometer, infrared spectral coverage spectrometer, master control and data analysis system, super continuous
Spectrum laser, infrared narrow linewidth laser and robot movement controller are powered.Master control and data analysis system are to right
It can be seen that spectral coverage spectrometer, infrared spectral coverage spectrometer, super continuous spectrums laser, infrared narrow linewidth laser and robot movement control
Device is controlled, and the spectroscopic data progress of visible spectral coverage spectrometer and the output of infrared spectral coverage spectrometer is received by USB interface
Processing and analysis.Master control and data analysis system included touch screen human-computer interaction interface receive for the human-computer interaction with user
The instruction of user simultaneously exports result to user.
Based on infrared Raman combine the super continuous unrestrained sealing blood identifier for integrating laser spectrum blood analysis method its
Step is：
(1) instrument starts and super continuous spectrums test sample introduction
Power supply module is opened, to visible spectral coverage spectrometer, infrared spectral coverage spectrometer, master control and data analysis system, super company
Continuous spectrum laser, infrared narrow linewidth laser and robot movement controller are powered.
User starts test main program by touch screen human-computer interaction interface.At this point, master control and data analysis system are sent out
Sample introduction, which instructs, gives robot movement controller, and robot movement controller controls test tube manipulator and captures test tube sample introduction to it along dress
It unloads major axes orientation and enters integrating sphere.Test tube bottom position is higher than transmitting optical axis and non-intersecting with optical axis is received, and avoids laser beam straight
Irradiation test tube to be checked is connect, and the transflector light of test tube is avoided directly to transmit along transmitting optical axis and collected by Two In and Two Out optical fiber.
(2) super continuous spectrums laser overflows integrated spectral test
Master control and data analysis system send out instruction and start super continuous spectrums laser, visible spectral coverage spectrometer and infrared spectral coverage
Spectrometer.The super continuous spectrums collimated laser beam of super continuous spectrums laser output enters integrating sphere, laser beam irradiation along transmitting optical axis
To after diffusing reflection coating, reflected light is diffusing reflection, i.e., is transmitted along all directions in integrating sphere, become uniform light.Test tube
After by the super continuous spectrums light irradiation of different directions, test tube (note：Including its material and outer labelling) it is unrestrained anti-with blood to be checked
Penetrate, diffusing transmission, absorption, the light after transmitting will be transmitted along space any direction, encounter the diffusing reflection of diffusing reflection coating to arbitrary side
To on the light transmission in integrating sphere with unrestrained integrated spectral influence.
After the diffusing reflection light of transmitting optical axis transmission is collected by Two In and Two Out optical fiber, sent respectively to visible spectral coverage spectrometer
And infrared spectral coverage spectrometer carries out opto-electronic conversion and becomes spectroscopic data.It can be seen that the sampled point of spectral coverage spectrometer is N1 (sampling numbers
Desirable N1=1300).The sampled point of infrared spectral coverage spectrometer is N2 (sampling number can use N2=512).It can be seen that spectral coverage spectrometer
And the total N=N1+N2 spectroscopic data of infrared spectral coverage spectrometer output is sent through USB interface to master control and data analysis system progress
Storage.
(3) Raman spectrum tests sample introduction
Master control and data analysis system, which send out sample and instruct, gives robot movement controller, the control of robot movement controller
Test tube machinery hand-motion test tube removes integrating sphere and sample room to it along handling major axes orientation, until Raman spectrum test point is located at examination
The center of blood to be checked in pipe completes Raman spectrum and tests sample introduction at this time.
(4) infrared laser Raman spectrum is tested
Master control and data analysis system send out instruction and start infrared narrow linewidth laser, visible spectral coverage spectrometer and INFRARED SPECTRUM
Section spectrometer.The collimated focusing post-concentration of infrared continuous laser beam that infrared narrow linewidth laser is sent out is to Raman spectrum test point
The blood to be checked at place, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced is successively through microcobjective and light
It is collected by the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. Raman reception optical fiber arrangement after fine collimating mirror, then through Raman
Reception optical fiber, Two In and Two Out optical fiber to infrared spectral coverage spectrometer carry out opto-electronic conversion and become spectroscopic data, are used to Raman spectrum
Sampling location and sampling number as infrared spectral coverage, i.e. N3=N2 are tested with super continuous spectrums.Infrared spectral coverage spectrometer output
N3 spectroscopic data, which is sent through USB interface to master control and data analysis system, to be stored.
(5) data analysis and fusion
The super continuous spectrums laser of infrared spectral coverage is overflow to N3 spectrum of the N2 spectroscopic data and Raman spectrum of integrated spectral
Data are weighted superposition, obtain the fusion spectroscopic data of N2 infrared spectral coverages.Wherein the weighted value of Raman spectrum is L, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-L.
By the N1 of the unrestrained integrated spectral of super continuous spectrums laser of the fusion spectroscopic data and visible spectral coverage of this N2 infrared spectral coverages
A spectroscopic data, N number of spectroscopic data is for subsequent analysis altogether.Based on Principal Component Analysis (principal components
Analysis, abbreviation PCA) M principal component numerical value of this N number of spectroscopic data is calculated, carry out dimension-reduction treatment.
(6) blood differentiates and judges
By the M principal component numerical value (number of principal components M can be taken as 7) of blood to be checked, obtains it and tie up the spy in principal component space in M
Sign vector, by the people obtained by this blood identifier and animal's whole blood, blood plasma, blood in this feature vector and M dimension principal components space
The cluster centre feature vector of clear M dimensions principal component spatial database is compared, and is waited for according to the determination first of feature vector similarity
The type blood for examining blood, is whole blood, blood plasma or serum.Then, then by M principal component numerical value of blood to be checked, with this
The cluster centre feature vector that the M of people and animal different genera under type blood tie up principal component spatial database compares,
Kind is determined again according to feature vector similarity, is people or animal blood, is which kind of animal blood if it is animal blood
Liquid so far completes the type blood of blood to be checked and the judgement of kind.
Then, the infrared laser of blood to be checked is induced Raman spectrum and visible infrared excess to connect by master control and data analysis system
The curve of spectrum and judgement result of continuous unrestrained comprehensive laser spectrum are shown on touch screen human-computer interaction interface, so that user joins
It examines.So far entire test process is completed.
The invention has the advantages that infrared narrow linewidth Ramar laser is used in infrared Raman spectral detection, in conjunction with
Fiber optic collimator and microcobjective focus to blood sample in test tube, and receive coaxial optical fiber end face design acquisition blood using transmitting
Sample backward Raman scattering signal effectively improves the signal-to-noise ratio of blood laser infrared raman spectral signal in test tube；Using infrared
Raman spectrum can effectively eliminate the fluorescence interference of blood sample in test tube；Using the integrating sphere of special designing, either blood plasma, blood
Hair is penetrated and is overflow in the diffusing reflection of the diffusing transmission of clear prescribed liquid or whole blood and test tube label, and the unrestrained suction of entire test sample
The wide range segment signal penetrated can receive, you can be suitable for all kinds of blood, all kinds of material test tubes, all kinds of different anti-coagulants and difference
Spectroscopic data under label condition uniformly acquires.Use the visible infrared high power super continuous spectrums lasing light emitter into and corresponding pair
Spectral coverage spectrometer obtains the unrestrained comprehensive laser spectrum data of wide spectrum, can be captured to the subtle optical difference for encapsulating blood, can
It improves and differentiates accuracy；Hardware uses Y shape fiberoptic connection Two In and Two Out optical fiber multiplexing spectrometer, and weighted superposition is used on software,
Realize the fusion of spectroscopic data information.Due to the robustness that hardware configuration and software are analyzed, blood identifier of the invention is applicable
In the automatic identification of whole blood, blood plasma and serum.
Description of the drawings
Fig. 1 is the principle of the present invention figure, wherein (a) super continuous spectrums laser overflows integrated spectral test；(b) infrared laser Raman
Spectrum test；(c) 1 figure of fibre profile；(d) 2 figure of fibre profile；(e) 3 figure of fibre profile.In figure：1 --- power supply module；
2 --- visible spectral coverage spectrometer；3 --- infrared spectral coverage spectrometer；4 --- spectrometer optical fiber interface；5 --- Two In and Two Out light
It is fine；6 --- USB interface；7 --- master control and data analysis system；8 --- integrating sphere optical fiber interface；9 --- integral spherojoint；
10 --- test tube manipulator；11 --- connecting rod；12 --- guide rail；13 --- robot movement controller；14 --- test tube cap；
15 --- test tube；16 --- circular hole；17 --- integrating sphere sample well；18 --- sample room outer cover plate；19 --- blood to be checked；
20 --- receive optical axis；21 --- sample room；22 --- bottom plate；23 --- integrating sphere right side；24 --- diffusing reflection coating；
25 --- transmitting optical axis；26 --- integrating sphere left side；27 --- light source chamber；28 --- integrating sphere light source hole；29 --- holder；
30 --- light source chamber outer cover plate；31 --- fiber optic collimator connector；32 --- laser tail optical fiber；33 --- touch screen human-computer interaction circle
Face；34 --- super continuous spectrums laser；35 --- integrating sphere；36 --- handling main shaft；37 --- infrared laser optical axis；38——
Raman spectrum test point；39 --- objective lens support；40 --- microcobjective；41 --- object lens connector；42 --- fiber optic collimator
Mirror；43 --- Y shape coaxial optical fiber；44 --- fiber optic connector；45 --- infrared emission optical fiber；46 --- Raman reception optical fiber；
47 --- Raman reception optical fiber arranges；48 --- infrared emission optical fiber arranges；49 --- infrared narrow linewidth laser.
Specific implementation mode
The hardware system structure of the sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on infrared Raman such as
Shown in Fig. 1, hardware system is mainly by power supply module 1, visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, Two In and Two Out optical fiber
5, master control and data analysis system 7, integrating sphere 35, test tube manipulator 10, connecting rod 11, guide rail 12, robot movement controller
13, sample room outer cover plate 18, bottom plate 22, holder 29, light source chamber outer cover plate 30, fiber optic collimator connector 31, laser tail optical fiber 32, super
Continuous spectrum laser 34, objective lens support 39, microcobjective 40, object lens connector 41, fiber optic collimator mirror 42, Y shape coaxial optical fiber 43,
Fiber optic connector 44, infrared narrow linewidth laser 49 form.
Wherein, integrating sphere 35 is made of integrating sphere right side 23 and the hemisphere of integrating sphere left side 26 two, they pass through product
Bulb separation connector 9 is coupled, and is fixed on bottom plate 22.35 inner wall of integrating sphere coats diffusing reflection coating 24, to being irradiated to
The light of inner wall plays the even light of diffusing reflection.Integrating sphere right side 23 is provided with integrating sphere sample well 17, sample room outer cover plate 18
On bottom plate 22 and integrating sphere right side 23, enclosure space sample room 21 is formed, to eliminate the influence of stray light.Sample room
The top of outer cover plate 18 is provided with circular hole 16, and handling main shaft 36 passes through the center of integrating sphere sample well 17 and circular hole 16, and with bottom plate 22
Vertically.Integrating sphere left side 26 is provided with integrating sphere light source hole 28 and the (note of integrating sphere optical fiber interface 8：It is SMA905 in the present embodiment
Interface).
Y shape coaxial optical fiber 43 is made of infrared emission optical fiber 45 and Raman reception optical fiber 46, and the two pools an optical fiber,
Its fiber end face is coaxial distribution, and central circular is that infrared emission optical fiber arranges 48, and outer concentric circular ring region is Raman
Reception optical fiber arrangement 47, the geometric configuration can efficient reception infrared laser excitation backward Raman scattering signal.Y shape coaxial optical fiber
43 couple achievable collimation transmitting with fiber optic collimator mirror 42 and receive, and fiber optic collimator mirror 42 passes through object lens connector 41 and micro- object
Mirror 40 is connected, and infrared laser beam can be focused to Raman spectrum test point 38 and to the back scattering of Raman spectrum test point 38
Signal is collected.Microcobjective 40 is fixedly installed on bottom plate 22 by objective lens support 39.
It can be seen that spectral coverage spectrometer 2 is all made of the (note of same light spectrometer optical fiber interface 4 with infrared spectral coverage spectrometer 3：The present embodiment
In be SMA905 interfaces), Two In and Two Out optical fiber 5 is divided to for two receiving terminals (i.e. two into) and two output ends (i.e. scene 2).One
Receiving terminal couples with integrating sphere optical fiber interface 8, collects from the diffusing reflection light for receiving optical axis 20；Another receiving terminal and drawing
Graceful reception optical fiber 46 couples (note by fiber optic connector 44：It is SMA905 connectors in the present embodiment), it collects and comes from Raman
The Raman signal of reception optical fiber 46；Two output ends couple with visible spectral coverage spectrometer 2 and infrared spectral coverage spectrometer 3 respectively.
(the note of super continuous spectrums laser 34：Its spectral region 400nm-2400nm in the present embodiment, power 1W, repetition 1MHz,
Pulsewidth 150ps) the visible and infrared spectral coverage super continuous spectrums pulse laser that sends out transmits through laser tail optical fiber 32, then pass through optical fiber
It collimates after connector 31 carries out beam collimation and exports super continuous spectrums pulse collimated laser beam, and integrating sphere light is passed through along transmitting optical axis 25
Source aperture 28 enters integrating sphere 35.
Fiber optic collimator connector 31 is fixed on by holder 29 on bottom plate 22, and passes through light source chamber outer cover plate 30 and integrating sphere left half
Portion's 26 shell connection, forms enclosure space light source chamber 27, to eliminate the influence of stray light.
The infrared continuous laser beam that infrared narrow linewidth laser 49 is sent out is through the infrared emission optical fiber in Y shape coaxial optical fiber 43
45 transmission collimate along the transmission of infrared laser optical axis 37, through fiber optic collimator mirror 42 after emitting in arranging 48 from infrared emission optical fiber, are aobvious
Infrared induction Raman excitation (the note to Raman spectrum test point 38 can be achieved in speck mirror 40 after focusing：Infrared laser optical axis 37 with
Load and unload main shaft 36 to intersect, intersection point is Raman spectrum test point 38), the back scattering Raman signal of Raman spectrum test point 38 according to
It is secondary to be connect by the outer concentric circular ring region of 43 end face of Y shape coaxial optical fiber, i.e. Raman after microcobjective 40 and fiber optic collimator mirror 42
Receive optical fiber arrangement 47 collect, then through Raman reception optical fiber 46, Two In and Two Out optical fiber 5 to infrared spectral coverage spectrometer 3 carry out receive and
Analysis.
Guide rail 12 and 22 right angle setting of bottom plate, test tube manipulator 10 are coupled with guide rail 12 by connecting rod 11 and can be in machineries
Hands movement controller 13 slides under controlling along guide rail 12.Blood 19 to be checked is encapsulated in by test tube cap 14 in test tube 15.Test tube machinery
Hand 10 can firmly grasp test tube cap 14 and drive test tube 15 above and below 36 direction of handling main shaft under the control of robot movement controller 13
Movement.
Power supply module 1 is to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, master control and data analysis system 7, super
Continuous spectrum laser 34, infrared narrow linewidth laser 49 and robot movement controller 13 are powered.Master control and data analysis
System 7 is to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, super continuous spectrums laser 34, infrared narrow linewidth laser
49 and robot movement controller 13 controlled, and visible spectral coverage spectrometer 2 and infrared spectral coverage light are received by USB interface 6
The spectroscopic data that spectrometer 3 exports is handled and is analyzed.7 included touch screen human-computer interaction interface of master control and data analysis system
33, for the human-computer interaction with user, receives the instruction of user and export result to user.
Based on infrared Raman combine the super continuous unrestrained sealing blood identifier for integrating laser spectrum blood analysis method its
Step is：
(1) instrument starts and super continuous spectrums test sample introduction
Open power supply module 1, to visible spectral coverage spectrometer 2, infrared spectral coverage spectrometer 3, master control and data analysis system 7,
Super continuous spectrums laser 34, infrared narrow linewidth laser 49 and robot movement controller 13 are powered.
User starts test main program by touch screen human-computer interaction interface 33.At this point, master control and data analysis system 7 are sent out
Go out sample introduction to instruct to robot movement controller 13, robot movement controller 13 control test tube manipulator 10 capture test tube 15 into
Sample enters integrating sphere 35 to it along 36 direction of handling main shaft.15 bottom position of test tube higher than transmitting optical axis 25 and with receive optical axis 20
It is non-intersecting, laser beam direct irradiation test tube 15 to be checked is avoided, and avoid the transflector light of test tube 15 directly along transmitting optical axis 25
It transmits and is collected by Two In and Two Out optical fiber 5.
(2) super continuous spectrums laser overflows integrated spectral test
As shown in Figure 1a, master control and data analysis system 7 send out instruction startup super continuous spectrums laser 34, visible spectral coverage light
Spectrometer 2 and infrared spectral coverage spectrometer 3.The super continuous spectrums collimated laser beam that super continuous spectrums laser 34 exports along transmitting optical axis 25 into
Enter integrating sphere 35, after laser beam is irradiated to diffusing reflection coating 24, reflected light is diffusing reflection, i.e., along all directions in integrating sphere 35
Transmission, becomes uniform light.Test tube 15 is after by the super continuous spectrums light irradiation of different directions, (the note of test tube 15：Including its material
With outer labelling) it will be transmitted along space any direction with the light after the diffusing reflection of blood 19 to be checked, diffusing transmission, absorption, transmitting,
24 diffusing reflection of diffusing reflection coating is encountered to any direction, there is unrestrained integrated spectral to influence the light transmission in integrating sphere 35.
After the diffusing reflection light transmitted along transmitting optical axis 25 is collected by Two In and Two Out optical fiber 5, sent respectively to visible spectral coverage light
Spectrometer 2 and infrared spectral coverage spectrometer 3 carry out opto-electronic conversion and become spectroscopic data.Visible spectral coverage spectrometer 2 in this specific embodiment
Spectral region be 400-750nm, sampled point N1=1300.The spectral region of infrared spectral coverage spectrometer 3 is 800-1750nm,
Sampled point is N2=512.It can be seen that spectral coverage spectrometer 2 and the total N=N1+N2 spectroscopic data warp of infrared spectral coverage spectrometer 3 output
USB interface 6 send to master control and data analysis system 7 and is stored.
(3) Raman spectrum tests sample introduction
Master control and data analysis system 7 send out sample and instruct to robot movement controller 13, robot movement controller
13 control test tube manipulators 10 drive test tube 15 to remove integrating sphere 35 and sample room 21 along 36 direction of handling main shaft to it, until Raman
Spectrum test point 38 is located at the center of blood 19 to be checked in test tube 15, completes Raman spectrum at this time and tests sample introduction.
(4) infrared laser Raman spectrum is tested
As shown in Figure 1 b, master control and data analysis system 7 send out the infrared narrow linewidth laser 49 of instruction startup, visible spectral coverage
Spectrometer 2 and infrared spectral coverage spectrometer 3.(the note of infrared narrow linewidth laser 49：Used in the present embodiment wavelength for 785nm ±
The semiconductor pumped solid continuous wave laser of 1nm, power 0.3W) the collimated focusing post-concentration of infrared continuous laser beam that sends out arrives
Blood to be checked 19 at Raman spectrum test point 38, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced
Successively by the outer concentric circular ring region of 43 end face of Y shape coaxial optical fiber, i.e. Raman after microcobjective 40 and fiber optic collimator mirror 42
Reception optical fiber arrangement 47 is collected, then carries out photoelectricity through Raman reception optical fiber 46, Two In and Two Out optical fiber 5 to infrared spectral coverage spectrometer 3
It converts and becomes spectroscopic data, the spectral region of infrared spectral coverage spectrometer 3 is 800-1750nm in this specific embodiment, to Raman light
Spectrum tests sampling location and sampling number as infrared spectral coverage, i.e. N3=N2=512 using with super continuous spectrums.Due to infrared narrow
The wavelength of line width laser 49 is located at except the spectral region of infrared spectral coverage spectrometer 3, therefore infrared laser echo does not interfere with
Acquisition to raman spectral signal inhibits echo interference without Rayleigh optical filter.N3 that infrared spectral coverage spectrometer 3 exports
Spectroscopic data send to master control and data analysis system 7 through USB interface 6 and is stored.
(5) data analysis and fusion
The super continuous spectrums laser of infrared spectral coverage is overflow to N3 spectrum of the N2 spectroscopic data and Raman spectrum of integrated spectral
Data are weighted superposition, obtain the fusion spectroscopic data of N2 infrared spectral coverages.Wherein the weighted value of Raman spectrum is L, surpasses and connects
The weighted value that continuous spectrum laser overflows integrated spectral is 1-L.(note：L=0.4 in the present embodiment)
By the N1 of the unrestrained integrated spectral of super continuous spectrums laser of the fusion spectroscopic data and visible spectral coverage of this N2 infrared spectral coverages
A spectroscopic data, N number of spectroscopic data is for subsequent analysis altogether.Based on Principal Component Analysis (principal components
Analysis, abbreviation PCA) calculate this N number of spectroscopic data M principal component numerical value (note：M=7 in the present embodiment calculates 7
A principal component numerical value), carry out dimension-reduction treatment.
(6) blood differentiates and judges
By M principal component numerical value of blood 19 to be checked, obtains it and tie up the feature vector in principal component space in M, by this feature
The people that by this blood identifier is obtained and animal's whole blood, blood plasma, serum M dimension principal component space of the vector with M dimension principal components space
The cluster centre feature vector of database is compared, and determines the blood class of blood 19 to be checked first according to feature vector similarity
Type is whole blood, blood plasma or serum.Then, then by M principal component numerical value of blood 19 to be checked, under the type blood
The cluster centre feature vector that people and the M of animal different genera tie up principal component spatial database compares, according to feature vector
Similarity determines kind again, is people or animal blood, is which kind of animal blood if it is animal blood, so far, completes to wait for
Examine the judgement of the type blood and kind of blood 19.
Then, master control and data analysis system 7 induce the infrared laser of blood 19 to be checked outside Raman spectrum and visible red
The curve of spectrum and judgement result of super continuous unrestrained comprehensive laser spectrum are shown on touch screen human-computer interaction interface 33, for
Family refers to.So far entire test process is completed.
Claims (1)
1. a kind of blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman, it includes power supply module (1), visible spectral coverage light
Spectrometer (2), infrared spectral coverage spectrometer (3), Two In and Two Out optical fiber (5), master control and data analysis system (7), integrating sphere (35), examination
Pipe manipulator (10), connecting rod (11), guide rail (12), robot movement controller (13), sample room outer cover plate (18), bottom plate
(22), holder (29), light source chamber outer cover plate (30), fiber optic collimator connector (31), laser tail optical fiber (32), super continuous spectrums laser
(34), objective lens support (39), microcobjective (40), object lens connector (41), fiber optic collimator mirror (42), Y shape coaxial optical fiber (43),
Fiber optic connector (44), infrared narrow linewidth laser (49), it is characterised in that：
The integrating sphere (35) is made of integrating sphere right side (23) and the hemisphere of integrating sphere left side (26) two, they pass through
Integral spherojoint (9) is coupled, and is fixed on bottom plate (22)；Integrating sphere (35) inner wall coats diffusing reflection coating
(24), play the even light of diffusing reflection to the light for being irradiated to inner wall；Integrating sphere right side (23) is provided with integrating sphere sample well
(17), sample room outer cover plate (18) is mounted on bottom plate (22) and integrating sphere right side (23), forms enclosure space sample room
(21), to eliminate the influence of stray light；Circular hole (16) is provided with above sample room outer cover plate (18), handling main shaft (36) passes through integral
The center of ball sample well (17) and circular hole (16), and it is vertical with bottom plate (22)；Integrating sphere left side (26) is provided with integrating sphere light source
Hole (28) and integrating sphere optical fiber interface (8)；
The Y shape coaxial optical fiber (43) is made of infrared emission optical fiber (45) and Raman reception optical fiber (46), and the two pools
A piece optical fiber, fiber end face are coaxial distribution, and central circular is that infrared emission optical fiber arranges (48), outer concentric circular ring
Region be Raman reception optical fiber arrange (47), the geometric configuration can efficient reception infrared laser excitation backward Raman scattering letter
Number；Y shape coaxial optical fiber (43) is coupled with fiber optic collimator mirror (42) can be achieved collimation transmitting and receives, and fiber optic collimator mirror (42) passes through
Object lens connector (41) is connected with microcobjective (40), and infrared laser beam can be focused to Raman spectrum test point (38) and right
The backscatter signal of Raman spectrum test point (38) is collected；Microcobjective (40) is fixedly installed in by objective lens support (39)
On bottom plate (22)；
The visible spectral coverage spectrometer (2) and infrared spectral coverage spectrometer (3) are all made of same light spectrometer optical fiber interface (4), and two
It is divided into two receiving terminals and two output ends into scene 2 optical fiber (5)；One receiving terminal couples with integrating sphere optical fiber interface (8), can
It collects from the diffusing reflection light for receiving optical axis (20)；Another receiving terminal passes through fiber optic connector with Raman reception optical fiber (46)
(44) couple, collect the Raman signal from Raman reception optical fiber (46)；Two output ends respectively with visible spectral coverage spectrometer
(2) and infrared spectral coverage spectrometer (3) couples；
The visible and infrared spectral coverage super continuous spectrums pulse laser that the super continuous spectrums laser (34) is sent out is through laser tail optical fiber
(32) it transmits, super continuous spectrums pulse collimated laser beam is exported after then carrying out beam collimation by fiber optic collimator connector (31), and
Integrating sphere light source hole (28) are passed through to enter integrating sphere (35) along transmitting optical axis (25)；
The fiber optic collimator connector (31) is fixed on by holder (29) on bottom plate (22), and by light source chamber outer cover plate (30) with
Integrating sphere left side (26) shell couples, and enclosure space light source chamber (27) is formed, to eliminate the influence of stray light；
The wavelength of the infrared narrow linewidth laser (49) is located at except the spectral region of infrared spectral coverage spectrometer (3), hair
The infrared continuous laser beam gone out is transmitted through the infrared emission optical fiber (45) in Y shape coaxial optical fiber (43), is arranged from infrared emission optical fiber
It arranges in (48) and is transmitted along infrared laser optical axis (37) after transmitting, after fiber optic collimator mirror (42) collimation, microcobjective (40) focus
The infrared induction Raman excitation to Raman spectrum test point (38) can be achieved；Infrared laser optical axis (37) and handling main shaft (36) phase
It hands over, intersection point is Raman spectrum test point (38), and the back scattering Raman signal of Raman spectrum test point (38) is successively through micro-
It is received by the outer concentric circular ring region of Y shape coaxial optical fiber (43) end face, i.e. Raman after object lens (40) and fiber optic collimator mirror (42)
Optical fiber arranges (47) and collects, then is carried out through Raman reception optical fiber (46), Two In and Two Out optical fiber (5) to infrared spectral coverage spectrometer (3)
It receives and analyzes；
The guide rail (12) and bottom plate (22) right angle setting, test tube manipulator (10) are joined by connecting rod (11) and guide rail (12)
It connects and can be slided along guide rail (12) under robot movement controller (13) control；Blood (19) to be checked is encapsulated by test tube cap (14)
In test tube (15)；Test tube manipulator (10) can firmly grasp test tube cap (14) and band under robot movement controller (13) control
Dynamic test tube (15) moves up and down in handling main shaft (36) direction；
The power supply module (1) is to visible spectral coverage spectrometer (2), infrared spectral coverage spectrometer (3), master control and data analysis
System (7), super continuous spectrums laser (34), infrared narrow linewidth laser (49) and robot movement controller (13) are supplied
Electricity；Master control and data analysis system (7) are to sharp to visible spectral coverage spectrometer (2), infrared spectral coverage spectrometer (3), super continuous spectrums
Light device (34), infrared narrow linewidth laser (49) and robot movement controller (13) are controlled, and pass through USB interface (6)
The spectroscopic data for receiving visible spectral coverage spectrometer (2) and infrared spectral coverage spectrometer (3) output is handled and is analyzed；Master control and number
Receive the instruction of user simultaneously for the human-computer interaction with user according to analysis system (7) included touch screen human-computer interaction interface (33)
Result is exported to user；
Master control and data analysis system send out instruction and start super continuous spectrums laser, visible spectral coverage spectrometer and infrared spectral coverage spectrum
The super continuous spectrums collimated laser beam of instrument, the output of super continuous spectrums laser enters integrating sphere along transmitting optical axis, and laser beam is irradiated to unrestrained
After reflectance coating, reflected light is diffusing reflection, after test tube is irradiated by the super continuous spectrums light of different directions, test tube, test tube outer patch
Light after the diffusing reflection of label and blood to be checked, diffusing transmission, absorption, transmitting will transmit along space any direction, encounter it is unrestrained instead
Coating diffusing reflection is penetrated to any direction, there is unrestrained integrated spectral to influence the light transmission in integrating sphere；It is passed along transmitting optical axis
After defeated diffusing reflection light is collected by Two In and Two Out optical fiber, sent respectively to visible spectral coverage spectrometer and the progress of infrared spectral coverage spectrometer
Opto-electronic conversion becomes spectroscopic data；Master control and data analysis system send out instruction and start infrared narrow linewidth laser, visible spectral coverage
Spectrometer and infrared spectral coverage spectrometer, the collimated focusing post-concentration of infrared continuous laser beam that infrared narrow linewidth laser is sent out arrive
Blood to be checked at Raman spectrum test point, the Stokes Raman spectral signal that infrared narrow linewidth continuous laser is induced is successively
It is arranged by the outer concentric circular ring region of Y shape coaxial optical fiber end face, i.e. Raman reception optical fiber after microcobjective and fiber optic collimator mirror
Row are collected, then are carried out opto-electronic conversion through Raman reception optical fiber, Two In and Two Out optical fiber to infrared spectral coverage spectrometer and become spectroscopic data；
It can be seen that spectral coverage spectrometer and the spectroscopic data of infrared spectral coverage spectrometer output through USB interface send to master control and data analysis system into
Row storage, analyzing processing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610167545.0A CN105866095B (en) | 2016-03-23 | 2016-03-23 | Blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610167545.0A CN105866095B (en) | 2016-03-23 | 2016-03-23 | Blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman |
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| CN105866095A CN105866095A (en) | 2016-08-17 |
| CN105866095B true CN105866095B (en) | 2018-10-19 |
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| CN201610167545.0A Active CN105866095B (en) | 2016-03-23 | 2016-03-23 | Blood identifier based on the super continuous unrestrained comprehensive spectrum of infrared Raman |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107907501B (en) * | 2017-10-13 | 2020-02-14 | 中国科学院上海技术物理研究所 | Cryopreservation whole blood analysis method of constant low-temperature supercontinuum |
| CN107643254B (en) * | 2017-10-13 | 2020-02-14 | 中国科学院上海技术物理研究所 | Cryopreserved whole blood analysis method based on time domain resolution supercontinuum |
| CN107688002B (en) * | 2017-10-13 | 2020-01-10 | 中国科学院上海技术物理研究所 | Cryopreserved whole blood analyzer of invariable low temperature super continuous integrated spectrum |
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| US4975581A (en) * | 1989-06-21 | 1990-12-04 | University Of New Mexico | Method of and apparatus for determining the similarity of a biological analyte from a model constructed from known biological fluids |
| CN2567566Y (en) * | 2002-09-14 | 2003-08-20 | 芜湖市皖江光电仪器有限责任公司 | Infrared, ultraviolet investigating instrument |
| CN101189503A (en) * | 2005-03-31 | 2008-05-28 | C2诊断公司 | Optical device for blood analysis, analyzing apparatus equipped with same |
| CN102365047A (en) * | 2009-01-25 | 2012-02-29 | 特鲁塔奇科技公司 | System for noninvasive determination of alcohol in tissue |
| CN105358947A (en) * | 2013-02-14 | 2016-02-24 | 曾海山 | Integrated spectral probe for raman, reflectance and fluorescence spectral measurements |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7403804B2 (en) * | 2001-04-11 | 2008-07-22 | Trutouch Technologies, Inc. | Noninvasive determination of alcohol in tissue |
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| US4975581A (en) * | 1989-06-21 | 1990-12-04 | University Of New Mexico | Method of and apparatus for determining the similarity of a biological analyte from a model constructed from known biological fluids |
| CN2567566Y (en) * | 2002-09-14 | 2003-08-20 | 芜湖市皖江光电仪器有限责任公司 | Infrared, ultraviolet investigating instrument |
| CN101189503A (en) * | 2005-03-31 | 2008-05-28 | C2诊断公司 | Optical device for blood analysis, analyzing apparatus equipped with same |
| CN102365047A (en) * | 2009-01-25 | 2012-02-29 | 特鲁塔奇科技公司 | System for noninvasive determination of alcohol in tissue |
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