CN105624044A - Method for achieving culturing and production increasing of euglena gracilis and water body purification - Google Patents
Method for achieving culturing and production increasing of euglena gracilis and water body purification Download PDFInfo
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- CN105624044A CN105624044A CN201610119436.1A CN201610119436A CN105624044A CN 105624044 A CN105624044 A CN 105624044A CN 201610119436 A CN201610119436 A CN 201610119436A CN 105624044 A CN105624044 A CN 105624044A
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Abstract
The invention discloses a method for achieving culturing and production increasing of euglena gracilis and water body purification. The method comprises the following steps that algae seeds of the euglena gracilis are inoculated into an HUT culture medium, and culturing is conducted for one week at room temperature under 24-h light exposure; the obtained euglena gracilis is inoculated into a eutrophic water body which is rich in nitrogen and phosphorus, the euglena gracilis is placed under natural light exposure for growth in the daytime, and the euglena gracilis is irradiated through LED blue light of which the illuminance is 2,400 lxs, and the wavelength ranges from 460 nm to 470 nm at night, so that growth of the euglena gracilis is facilitated, and the growth cycle is 7-10 days. According to the method for achieving culturing and production increasing of the euglena gracilis and water body purification, high practicability is achieved, and the method can be mainly used for landscape ponds, culturing and production increasing of the euglena gracilis, purifying of the eutrophic water body and the like; compared with other method for facilitating growth of the euglena gracilis and purifying water bodies, the method has the advantages of being capable of saving energy, environmentally friendly, wide in range, low in cost, low in technical requirement, simple and easy to use.
Description
Technical field
The present invention relates to biological technical field, specifically refer to the method for the cultivation volume increase of a kind of very thin Euglena and purifying water body.
Background technology
Very thin Euglena is division euglenophyta, Euglena, is the protozoon living in fresh water. Become the environmental protection food of extensive concern since the 00's of 21st century across the series health-care products of the green Euglena (Euglena) of vegitabilia and animal kingdom, and start to be applied to the trial of agricultural, makeup and food raw material. Euglena is the minicell algae simultaneously having possessed plant and animal two kinds of features. The necessary amino acid of needed by human body is contained as breast milk. Euglena photosynthesis is strong, containing abundant nutrition composition. Acellular wall and balancedly containing the nutritive element of 59 kinds of needed by human, it is possible to comprehensively supplement the nutrition of needed by human body. Euglena can suppress purine (people's gout simultaneously, it is because having eaten a large amount of meat, adenyl-deoxyribonucleotide (dAMP Desoxyadenosine), guanine deoxyribonucleoside acid (dGMP pancreatic desoxyribonuclease) metabolism in meat DNA go wrong) absorb, utilize natural Euglena treatment gout also to realize. And very thin Euglena has strong photosynthesis, acellular wall, moves freely, the characteristic of nutritious property, has unprecedented research prospect.
The pH value cultivating very thin Euglena is advisable with 6.4. Warm season, water temperature was bred when 20��30 DEG C soon, but owing to substratum having yeast extract paste, therefore bacterium usually with algae competition, become the big difficult problem cultivating very thin Euglena.
Nowadays, along with the rapid development of China's economic, bringing a large amount of pollutions to water body environment, wherein, the phenomenon of body eutrophication is particularly serious. The improvement of body eutrophication is extremely urgent. The method administered is broadly divided into physics method, chemical method, biological process three kinds, and wherein chemical method is effective, but the side effect brought is greatly, and is not that people accept usually, and physics method is difficult to radical cure problem, and balance adopts biological process best and environmental injury is minimum.
Research according to forefathers, with spirulina, the improvement of body eutrophication is realized, but it is still not enough to some extent, because the absorption of the nutritive substance in water body is completed by spirulina by self adsorptivity, therefore after having purified water body, its use value can be lost, cause a large amount of wastes.
But very thin Euglena is different, the absorption of the Eutrophication materials of water body is completed by it by own absorption, that is, the nutritive ingredients such as those N, P can be absorbed by algae and by algae metabolism, the number of algae is increased, and experiment afterwards also demonstrates this result.
Therefore, it is a thing killed two birds with one stone by adding that water body purifies by very thin Euglena, it is possible not only to the environmental improvement making water body, it is possible to so that alga yield increasing.
Because LED/light source is cold light source, owing to electric energy can be converted into luminous energy by cold light source greatly, and non-thermal energy, therefore relatively conventional light source has the advantages such as life-span length, spectroscopically pure, energy consumption are low, will play a significant role in facility luminous environment regulates and controls. Now have and regulate and control the report of the research on the vegetables such as cherry tomato, cucumber, Leaf lettuce about vegetable in seedling period LED light on a small quantity, for the present invention provides exploitativeness.
Summary of the invention
Compared to spirulina, chlorella, when it as feed or during foodstuff additive, very thin Euglena has higher digestibility. Meanwhile, owing to it is to the good absorption ability of nitrogen phosphorus in water body, the waste water being rich in nitrogen phosphorus can be utilized in breeding process as the raw material of mass propgation. Therefore very thin Euglena also can be used for the nitrogen and phosphorous nutrient in purifying eutrophication water body. The rudimentary knowledge such as the culture condition studying very thin Euglena have very high potential value for the further Application and Development of this algae. The object of the present invention just is to propose the cultivation volume increase of a kind of very thin Euglena and the method for purifying water body, while the water body of eutrophication being purified, moreover it is possible to promote that algae carries out metabolism, impel the growth of algae, obtain the effect killed two birds with one stone.
The present invention is verified by research light the impact of cleaning action of the growth of very thin Euglena and eutrophication water, adopt controlled variable method, keep all the other illumination factors such as intensity of illumination, periodicity of illumination constant, the spectrum only controlling its light being subject to is different, and set up the one group of control group irradiated by fluorescent lamp to test, have found the physical method promoting the growth of very thin Euglena, and probed into and used when different light medium very thin Euglena on the impact of the cleaning action of eutrophic water body. The concrete technical scheme that the present invention obtains is:
The method of the cultivation volume increase of a kind of very thin Euglena and purifying water body, it is characterised in that, comprise the following steps:
(3) by, in the algae kind access HUT substratum of very thin Euglena, at room temperature, 24 h light are cultivated one week;
(4) the very thin Euglena that step (1) obtains is rich in the eutrophication water of nitrogen phosphorus, grow under making daytime it be placed in natural light irradiation, 2400lxs illumination is used when night is unglazed, it is irradiated by wavelength at the blue light of LED of 460-470nm, to promote the growth of very thin Euglena, growth around 7-10 days.
A preferred version of the present invention is: the ruddiness adding wavelength 645nm-655nm when phosphorus element content is higher relative to nitrogen element in water body irradiates, otherwise, adding wavelength at the blue light of 460-470nm irradiates.
Due to the effect difference to algae, therefore adjustable smooth matter condition, changes the speed of growth and the quality of algae. Therefore, another optimal technical scheme of the present invention is: needs algae to breed the ruddiness adding wavelength 645nm-655nm when increasing results frequency or be there are certain requirements by the carbohydrate content in frond fast and irradiates; Adding wavelength when algae protein matter content or lipid material content be there are certain requirements at the blue light of 460-470nm irradiates.
The present invention separately wards off path in the selection of algae, select studied number of times few, very thin Euglena rarely known by the people is studied, draw the growth curve of very thin Euglena under different light medium (photochromic) first, obtain a kind of method that very thin Euglena killed two birds with one stone cultivates volume increase and purifying water body.
The method of the present invention has very strong practicality, but it is mainly used in view pool, the cultivation volume increase of very thin Euglena, the purification etc. to eutrophication water, it is compared to the method that other promote the growth of very thin Euglena and purifying water body, have energy-conservation, environmental protection, scope is wide, low cost, technical requirements is low, feature easy to use.
Accompanying drawing explanation
The absorbance of very thin Euglena algae liquid under 665nm wavelength when Fig. 1 different light medium.
The absorbance of very thin Euglena algae liquid under 750nm wavelength when Fig. 2 different light medium.
Embodiment
The better embodiment of the present invention is provided, so that the technical scheme of the present invention to be described in detail below in conjunction with accompanying drawing.
Embodiment 1:
By in the algae kind of 5ml access 100mlHut substratum (controlling the substratum in all bottles and algae kind equivalent), control the illumination equal (2400lxs) that all Erlenmeyer flasks receive, control 12 h light, 12 h dark. Establish 4 groups altogether, it is respectively white light (fluorescent lamp) group, ruddiness group, blue light group and green glow group. Often organize spectrum and have 3 Erlenmeyer flasks (establish three parallel). Between 4 groups except light matter difference, the culture condition controls such as intensity of illumination, light application time, inoculum density are consistent.
Table 1
| # | Group part | Consumption |
| 1 | KH2PO4 | 0.02g/L |
| 2 | Peptone | 0.6g/L |
| 3 | MgSO4��7H2O | 0.025g/L |
| 4 | Yeast mannan | 0.4g/L |
| 5 | Sodium acetate | 0.4g/L |
| 6 | Vitamin B12 | 0.5ug/L |
| 7 | Tripotassium Citrate | 0.04g/L |
| 8 | VITMAIN B1 | 0.4mg/L |
pH6.4
Variable: often organize the light matter difference (red, blue, green) (ruddiness 645nm-655nm, green glow 550nm-560nm, blue light 460-470nm) that Erlenmeyer flask receives.
Algae OD value under every two days use twin-beam ultraviolet-visible pectrophotometer survey different light medium, draws growth curve, explores under the irradiation of different light medium, the growing state of algae and growth cycle. Its result is as illustrated in fig. 1 and 2. After experiment terminates, filter algae kind measure dry weight, contrast. Its result is as shown in table 2.
The very thin Euglena dry cell weight cultivated when table 2 different light medium
| White 1 | White 2 | White 3 | Green 1 | Green 2 | Green 3 | |
| Dry weight | 0.0066 | 0.0073 | 0.0068 | 0.002 | 0.0016 | 0.002 |
| Blue 1 | Blue 2 | Blue 3 | Red 1 | Red 2 | Red 3 | |
| Dry weight | 0.0054 | 0.0041 | 0.0044 | 0.0038 | 0.0055 | 0.005 |
Known when each condition is all identical by experiment, the growth of very thin Euglena is slightly better than ruddiness by blue LED lamplight, is greatly better than green glow.
Because in frustule, the photosynthetic pigment of different participation is different to the degree of absorption of the light of different light matter conditions. Such as Chlorophyll A dominant absorption blood orange light and royal purple light, Chlorophyll B is dominant absorption royal purple light then, xenthophylls and carotene then all dominant absorption royal purple light. And in green alga, especially in green algate of fresh water, the content of Chlorophyll A accounts for the overwhelming majority in all participation Photosynthesis Pigment, so the growth of very thin Euglena is slightly better than ruddiness by LED lamplight blue in an experiment. And ruddiness looks the fastest at the beginning of experiment, and stop growing the soonest be because red light wavelength long, energy is lower, can directly activate the electronics in Chlorophyll A and carry out photosynthesis, photosynthetic efficiency is the highest, makes algal grown speed fast, but due to the accessory pigment such as Chlorophyll B, xenthophylls and carotene cannot good absorptive red light, promoting algal grown, therefore the growth cycle of ruddiness is also the shortest.
And for green glow, Chlorophyll A almost cannot absorb, so the LED lamplight of green is very little to the promoter action of the growth of algae, in addition, by the lasting observation very thin Euglena of various light-illuminating carried out under microscope, finding that the very thin Euglena that green LED lamplight is irradiated is almost all dead, therefore, the growth of very thin Euglena is had restraining effect by green glow.
Embodiment 2:
With SODIUMNITRATE and potassium primary phosphate analog configuration eutrophication water, surveying the initial value of TP/TN, by algae centrifuging after cultivating when different light medium two weeks, the numerical evaluation algae surveying remaining TP/TN in water body is to the specific absorption of body eutrophication.
When different light medium, very thin Euglena is to the assimilation effect of phosphorus total in water body:
When table 3 different light medium, very thin Euglena is to the assimilation effect of phosphorus total in water body
When table 4 different light medium, very thin Euglena is to the assimilation effect of nitrogen total in water body
Can find out that the phosphoric in water body is had good assimilation effect by very thin Euglena by table 3 and table 4, the nitrogen unit in water body is have certain assimilation effect. To, in the assimilation effect of phosphoric, the effect of the algae that blue light irradiates and natural light are suitable, all close to 80%; And green glow also has certain effect to be about 36%. In the assimilation effect to nitrogen element, natural light is better, is 18.25%, but the assimilation effect of ruddiness and green glow is comparatively faint a little more than 10%.
Sum up:
The very thin Euglena shining blue light is best to the sorption of the N in water body, P element. The average data of dry weight is then ruddiness a little more than blue light, is much higher than green glow. And prove that the rich nutritive material in water body can be absorbed metabolism by very thin Euglena, and increase the number of self, and as spirulina, the effect of purifying water body is not reached by self adsorptivity.
Owing to red light is mainly absorbed by Chlorophyll A, and the photosynthesis that Chlorophyll A participates in is mainly algae accumulation carbohydrate, energy is provided to make it grow (breeding), therefore, in the water body that red light is irradiated, algae is higher to the specific absorption of the phosphoric that can form biological cell membrane. And the nitrogen element absorption effect being mainly used in synthetic protein is not good.
And under the irradiation of blue light, each pigment of algae can play a role, but due to blue light wave length, photon energy height, therefore must damping capacity become ruddiness after could accumulate the carbohydrate in algae, therefore dry weight does not have ruddiness weight.
More than show and describe the ultimate principle of the present invention, the advantage of main characteristic sum the present invention. The technician of the industry should understand; the present invention is not restricted to the described embodiments; the principle that the present invention is just described described in above-described embodiment and specification sheets; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.
Claims (3)
1. the method for a very thin Euglena cultivation volume increase and purifying water body, it is characterised in that, comprise the following steps:
(1) by, in the algae kind access HUT substratum of very thin Euglena, at room temperature, 24 h light are cultivated one week;
(2) the very thin Euglena that step (1) obtains is rich in the eutrophication water of nitrogen phosphorus, grow under making daytime it be placed in natural light irradiation, 2400lxs illumination is used when night is unglazed, it is irradiated by wavelength at the blue light of LED of 460-470nm, to promote the growth of very thin Euglena, growth around 7-10 days.
2. the method for very thin Euglena according to claim 1 cultivation volume increase and purifying water body, it is characterized in that, the ruddiness adding wavelength 645nm-655nm when phosphorus element content is higher relative to nitrogen element in water body irradiates, otherwise, adding wavelength at the blue light of 460-470nm irradiates.
3. the method for very thin Euglena according to claim 1 cultivation volume increase and purifying water body, it is characterized in that, it is necessary to algae breeds the ruddiness adding wavelength 645nm-655nm when increasing results frequency or be there are certain requirements by the carbohydrate content in frond fast and irradiates; Adding wavelength when algae protein matter content or lipid material content be there are certain requirements at the blue light of 460-470nm irradiates.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644860A (en) * | 2019-01-17 | 2019-04-19 | 深圳市千百辉照明工程有限公司 | Inhibit the new light sources of algal grown in a kind of illumination of solution cavity |
| CN112662563A (en) * | 2020-12-25 | 2021-04-16 | 暨南大学 | Culture medium for promoting microalgae to accumulate high value-added active substances and preparation method thereof |
| CN112680359A (en) * | 2020-12-25 | 2021-04-20 | 暨南大学 | Microalgae culture medium and application thereof |
| CN114621313A (en) * | 2020-12-10 | 2022-06-14 | 启迪禾美生物科技(嘉兴)有限公司 | Euglena protein extract and application thereof in cosmetics |
| CN117025402A (en) * | 2023-08-23 | 2023-11-10 | 任茂智 | Euglena gracilis capable of producing nano-selenium, application thereof and nano-selenium preparation method |
| CN118545843A (en) * | 2024-07-30 | 2024-08-27 | 中国农业科学院北京畜牧兽医研究所 | Purification and comprehensive utilization method for cattle farm fecal sewage |
| CN118545843B (en) * | 2024-07-30 | 2024-11-12 | 中国农业科学院北京畜牧兽医研究所 | Purification and comprehensive utilization method for cattle farm fecal sewage |
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| CN101633894A (en) * | 2009-08-20 | 2010-01-27 | 北京芳能科技有限公司 | Culture medium of euglena gracilis and open type high-density culture method |
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Patent Citations (1)
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| CN101633894A (en) * | 2009-08-20 | 2010-01-27 | 北京芳能科技有限公司 | Culture medium of euglena gracilis and open type high-density culture method |
Non-Patent Citations (2)
| Title |
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| KENTA TORIHARA ET AL: "Evaluation of Growth Characteristics of Euglena gracilis for Microalgal Biomass Production Using Wastewater", 《JOURNAL OF WATER AND ENVIRONMENT TECHNOLOGY》 * |
| TETSUAKI OSAFIINE ET AL: "Meets of Blue and Red Light on Chloroplast Development in Dark-Grown Wax-Rich Cells of Euglena gracilis Transferred to Inorganic Medium", 《J ELECTRON MLCROSC》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644860A (en) * | 2019-01-17 | 2019-04-19 | 深圳市千百辉照明工程有限公司 | Inhibit the new light sources of algal grown in a kind of illumination of solution cavity |
| CN114621313A (en) * | 2020-12-10 | 2022-06-14 | 启迪禾美生物科技(嘉兴)有限公司 | Euglena protein extract and application thereof in cosmetics |
| CN114621313B (en) * | 2020-12-10 | 2024-08-23 | 禾美生物科技(浙江)有限公司 | Euglena protein extract and its use in cosmetics |
| CN112662563A (en) * | 2020-12-25 | 2021-04-16 | 暨南大学 | Culture medium for promoting microalgae to accumulate high value-added active substances and preparation method thereof |
| CN112680359A (en) * | 2020-12-25 | 2021-04-20 | 暨南大学 | Microalgae culture medium and application thereof |
| CN117025402A (en) * | 2023-08-23 | 2023-11-10 | 任茂智 | Euglena gracilis capable of producing nano-selenium, application thereof and nano-selenium preparation method |
| CN117025402B (en) * | 2023-08-23 | 2024-11-15 | 任茂智 | Euglena gracilis capable of producing nano-selenium, application thereof and nano-selenium preparation method |
| CN118545843A (en) * | 2024-07-30 | 2024-08-27 | 中国农业科学院北京畜牧兽医研究所 | Purification and comprehensive utilization method for cattle farm fecal sewage |
| CN118545843B (en) * | 2024-07-30 | 2024-11-12 | 中国农业科学院北京畜牧兽医研究所 | Purification and comprehensive utilization method for cattle farm fecal sewage |
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