CN105614843B - A kind of nutrient protein food preparation processes and its application with anti-fatigue effect - Google Patents
A kind of nutrient protein food preparation processes and its application with anti-fatigue effect Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Beans For Foods Or Fodder (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a kind of nutrient protein food preparation processes and its application with anti-fatigue effect, and invention belongs to functional food field, the in particular to remarkable efficacy alleviated to sports fatigue.The Major Nutrient base-material of nutrient protein food prepared by the present invention includes soybean protein isolate and lactalbumin, according to nitrogen (N) content of every kind of albumen of detection in the nutrient protein food, the parts by weight of soybean protein isolate and lactalbumin form in the nutrient protein food are as follows: 44-46 parts of soybean protein isolate, 53-55 parts of lactalbumin；The present invention uses nutrient protein food (nitrogen is waited to match), pass through nutritional intervention after rats weight bearing hydrostatic swimming in seven weeks, lactic acid dehydrogenase activity in rat body can be significantly improved under the precursor for not increasing rat body weight, extended rat swimming with a load attached to the body power and exhausted the time.Have effects that significantly to alleviate sports fatigue, so that rat swimming with a load attached to the body power is exhausted average time and reach 135 seconds that there is good utility value in the exploitation of anti-fatigue effect food.
Description
Technical field:
The invention belongs to functional food fields, in particular to the remarkable efficacy alleviated to sports fatigue.
Background technique:
Vigorous exercise, high pressure and the allegro modern life can bring different degrees of fatigue.Motility
Fatigue refers to that organism physiology process cannot maintain it to complete scheduled exercise intensity.Fatigue, which cannot effectively be alleviated, causes muscle mistake
The material buildups such as degree anxiety, free radical, lactic acid finally influence movement effects.Therefore, how applied nutrition means are developed in recent years
Functional food, which alleviates sports fatigue, becomes research hotspot.
Lactalbumin belongs to high-quality protein, and containing eight kinds of amino acid necessary to human body, proportion meets human body requirements.Whey
Albumen is easy to be absorbed, under stomach acidic environment can rapid digestion degradation, it is a large amount of required to release in a relatively short period of time
Amino acid, especially using leucine as the branched-chain amino acid of representative.In addition, lactalbumin includes that beta lactoglobulin (accounts for about 45%-
48%), a large amount of bioactive substances such as α-lactalbumin (accounting for about 18%), immunoglobulin (accounting for about 8%) and lactoferrin.
Clinical research shows that lactalbumin intake can improve the expression of reduced glutathione, to improve immune response energy
Power.Albumin include such as ferritin, can be to free radical resisting, the effect of making it have anti-aging.
Soybean protein isolate component is single and cholesterol-free, and the soybean protein of 86%-88% belongs to water-solubility protein, main
If accounting for the 85% of water soluble protein based on globulin.Soybean protein contains 16 kinds of amino acid, is known today unique
Protein and rich content containing 9 kinds of essential amino acids, protein digestibility correction Acid proteins (PDCAAS) reach
Maximum value 1.In addition to methionine and cysteine content are less, remaining essential amino acids content reaches or surpasses soybean protein isolate
The level for the essential amino acid requirement that the Guo Liao World Health Organization (WHO) is recommended.It is different that soybean protein contains soya-bean polypeptides, soybean
Flavones, soyasaponin and various trace elements have extensive alimentary health-care function.Relatively biological regulation effect has certainly at present
Blood lipid is adjusted, blood glucose is adjusted, mitigates kidney burden, many effects such as adjusts blood pressure and prevent osteoporosis.
A kind of egg-pair white spirit and preparation method thereof application number: 201310280490.0 applyings date: the 2013-07-04 present invention
Belong to food processing technology field, discloses a kind of egg-pair white spirit and preparation method thereof, solve with egg, soybean protein isolate
Powder and/or the concentrated albumen-powder of soybeans are raw material, with first circulation hardness, targeted loads, viscous force, viscosity, elastic force, cohesion, bullet
Property, deadlocked property and chewiness be measurement index, design beat up eggs, deploy, boiling, stripping and slicing, baking, seasoning, vacuum packaging, sterilization
The egg-pair white spirit with high-quality nutrition and good physical property is made in the process route of technique.The present invention is excellent rich in animality by two kinds
Matter albumen and the mixing of vegetalitas high-quality protein raw material, have given full play to the complementation of food protein, have improved protein
Digestibility and nutritive value of food.Product has drawn the essence of boiling and baking, crisp outside and soft inside, avoid traditional dried eggs and
Special fishy smell possessed by dried bean curd, and the egg-pair white spirit of six kinds of tastes such as barbecue taste, beef flavour is produced, various differences can be met
The dietary requirements and taste of crowd are accustomed to.A kind of double-protein liquid milk and preparation method thereof, application number: 200910143367.8 Shens
Please day: 2009-05-25 the present invention relates to a kind of liquid milk, especially a kind of double-protein liquid milk for adding phytosterin ester and
Preparation method.Belong to dairy technology field.Double-protein liquid milk provided by the present invention, component and weight ratio are as follows:
Milk (or reconstituted milk) 885~995KG, emulsion stabilizer 2-5KG, 5~10KG of soybean protein isolate, phytosterin ester 0.5~
1.5KG, 0.5~3KG of essence, remaining is water.Milk in conjunction with soybean protein, while being added phytosterin ester by the present invention, is obtained
It is balanced to a kind of nutrition, be conducive to the double-protein liquid milk of cardioprotection.
Belong to animal protein from nutrition angle analysis lactalbumin, long-term consumption animal protein is be easy to cause in vivo
Cholesterol, fat and heat build-up have an adverse effect to body.Soybean protein isolate belongs to vegetable protein, long-term consumption with
Soybean is that the vegetable protein of representative be easy to cause methionine intake not enough, and vegetable protein contains anti-nutritional factors, they
It can influence absorption of the body to nutriment.Therefore, soybean and cow's milk protein animal/vegetable protein advantage how are made full use of,
Animal/vegetable protein mutual supplement with each other's advantages effect is played in terms of nutrition, taste, improves the comprehensive utilization ratio of animal/vegetable protein, and exploitation has good
The albumen food of good alimentary health-care function meets and improves people's sport nutrition health status, alleviates city crowd inferior health shape
State is the fields such as current sports science, Food Science and medicine urgent problem to be solved.
Summary of the invention:
Present invention solution technical problem, which is to provide one kind, can alleviate the functional protein nutriment of sports fatigue, and mention
Core preparation method for the functional protein food and its prove experimental data in the animal body of its effect.
The functional protein nutriment, comprising main component be the soybean protein that is extracted from Non-transgenic soybean and
The milk protein extracted from cow's milk.
The functional protein nutriment the preparation method is as follows:
Prepare the determination of principle: factor 1: amino acid release mode difference.By the soybean protein isolate of equivalent and whey egg
It is white to feed empty stomach wistar rat (Nutritional studies model) respectively, rat peripheral blood essential amino acid is detected by tandem mass spectrum
Dynamic release mode, higher by lactalbumin digestion rate as the result is shown, 30min amino acid discharges after gastric acid digests
Reach peak value, 60min amino acid just reaches peak value after soybean protein isolate intake.Factor 2: amino acid compositional model difference.Benefit
It is the unique high-quality egg containing 9 kinds of essential amino acids reported at present with Mass Spectrometer Method amino acid classes discovery soybean protein isolate
White, lactalbumin contains 8 kinds of essential amino acids.Factor 3: protein component difference.Soybean protein isolate contains 7S, 11s etc.
The protein isolate of main component, and lactalbumin belongs to uniform albumen.Fuzzy comprehensive estimation side is established based on above-mentioned three big factors
The possibility scheme of method evaluation optimal proportion is that two kinds of protein are matched in a manner of waiting nitrogen, passes through later period animal experiment in vivo
The nitrogen proportioning effect such as data verification is best, can utmostly guarantee the release of amino acid duration, amino after two kinds of albumen intakes
Acid composition type is comprehensive and functional 7s, 11s protein content is abundant.
A kind of nutrient protein food with anti-fatigue effect:
Technical solution is as follows:
The Major Nutrient base-material of nutrient protein food prepared by the present invention includes soybean protein isolate and lactalbumin, institute
State in nutrient protein food according to nitrogen (N) content of every kind of albumen of detection, in the nutrient protein food soybean protein isolate and
The parts by weight of lactalbumin form are as follows: 44-46 parts of soybean protein isolate, 53-55 parts of lactalbumin；
Preferably, the parts by weight of soybean protein isolate and lactalbumin form in the nutrient protein food are as follows: soybean
45-46 parts of protein isolate, 54-55 parts of lactalbumin；
Preferably, the parts by weight of soybean protein isolate and lactalbumin form in the nutrient protein food are as follows: soybean
45.4 parts of protein isolate, 54.6 parts of lactalbumin；
The key of fluid nutrient protein food the preparation method is as follows:
It weighs 45.4 parts of soybean protein isolate respectively according to parts by weight, 54.6 parts of lactalbumin, is sufficiently mixed after hydration
Nutrient protein food is made.
The nitrogen nutrient protein foods such as specific preparation 100g need soybean protein isolate 45.4g, lactalbumin 54.6g.Respectively
45.4g soybean protein isolate and 54.6g lactalbumin are weighed, is sufficiently mixed after hydration and both obtained.
Preferably various food products can be prepared by addition food auxiliary material on the basis of the nutrient protein food of above-mentioned configuration.
Dumars azotometer detects every gram of soybean protein isolate and lactalbumin nitrogen content NBeans=0.12 and NCream/ g=0.10,
The nitrogen nutrient protein foods such as specific preparation 100g need soybean protein isolate 45.4g, lactalbumin 54.6g.Respectively
45.4g soybean protein isolate and 54.6g lactalbumin are weighed, is sufficiently mixed after hydration and both obtained.
The rat experiment configuration of albumen food solns:
The nitrogen nutrient protein foods such as 100 milligrams, under conditions of 50 degrees Centigrade, 300rpm are added in every milliliter of pure water
Hydration 30 minutes, effect is best, and the nitrogen nutrient protein food such as 100g addition ultrapure water is configured to final concentration of 100mg/ml's
Equal nitrogen nutrient protein food solns.
Fluid nutrient protein food preparation processes: 100g fluid nutrient protein food, soybean protein isolate 45.4g, whey
Albumen 54.6g.Soybean protein isolate is weighed respectively to mix with lactalbumin, and 50 DEG C of warm water 300rpm is added and is hydrated 30 minutes,
Rotation 300rpm is carried out using magnetic bead rotor during hydration, the fluid nutrient protein of final concentration of 100mg/ml is made
Food.
Experiments have shown that this temperature hydration effect is best, the maxima solubility of soybean protein isolate and lactalbumin can be reached.
Meanwhile the formation of two kinds of albumen suspensions can be promoted in this way.The advantages of operation, is that two kinds of equal abundant water of nitrogen albumen can be made
Conjunction forms suspension, can be absorbed in the form of waiting nitrogen by pipe intestinal digesting after body intake.
A, it needs to be hydrated with 50 DEG C of warm water before soybean protein isolate is mixed with lactalbumin, experiments have shown that this temperature
That aspect effect of hydration effect is best, can reach the maxima solubility of soybean protein isolate and lactalbumin.Meanwhile in hydration
Rotation 300rpm, 30min are carried out using magnetic bead rotor in the process, the formation of two kinds of albumen suspensions can be promoted in this way.It should
The advantages of operation, is to make two kinds of equal nitrogen albumen to be sufficiently hydrated to form suspension, after body intake can in the form of equal nitrogen quilt
Pipe intestinal digesting absorbs.
B, nutrient protein food formulation solution using etc. nitrogen mode, soybean protein isolate and whey egg in nutrient protein food
It is white to be prepared according to equal nitrogen mode.Need by Dumars azotometer by soybean protein isolate prepared by the present invention and lactalbumin into
Row nitrogen analysis.Measurement result NSoybean protein isolate/ g=0.12 and NLactalbumin/ g=0.10, it is dry to prepare 100g nutrient protein food
Powder calculates, and needs soybean protein isolate 45.4g, lactalbumin 54.6g.It is dense according to nitrogen nutrient protein foods such as experimental data discoveries
Degree can effectively enhance rat anti-fatigue ability under the concentration of 200mg/2ml, extend walking weight load.
The soybean separation protein white matter preparation process is as follows: Non-transgenic soybean dregs of beans is obtained by alkali extraction method.Soybean
Distilled water mixing is added in dregs of beans, and mixed proportion is+90% distilled water of 10% dregs of beans, and NaOH is added after mixing, adjusts PH=7, molten
Liquid is centrifuged, centrifugal speed 3800rpm, centrifugation time 30min.Supernatant is taken, hydrochloric acid adjustment PH is added into supernatant extremely
4.5, after the protein such as standing are precipitated completely, 3800rpm is centrifuged 30min, abandons supernatant, takes precipitating.NaOH is added into sediment
Make PH=7, by being dried by spraying.It is greater than 90% by detection protein content, moisture teller detection moisture is less than
7%, ash content determinator is detected less than 6%.
The milk protein preparation process is as follows: extracting crude protein in cow's milk using 25% dehydrated alcohol, gained is filtered
Dehydrated alcohol is added according to 2.8:1 ratio in liquid, after standing 30min minutes, obtains lactalbumin with 4000rpm centrifugation 30min, passes through
Cross clarification, ultrafiltration, dry acquisition whey protein concentrate.It is greater than 90% by detection protein content, moisture teller detects water
Divide less than 8%, ash content determinator is detected less than 5%.
The utility model has the advantages that
Soybean protein isolate and lactalbumin are deployed into nutrient protein food by equal nitrogen mode by the present invention, are scheduled to last
Seven weeks wistar rats'swimmings move Intervention trial.Feed is maintained using free diet and restricted scale of rationing rat in test, often
It carry out swimming instruction, training after four groups carry out respectively equivalent lactalbumin, soybean protein isolate, etc. nitrogen nutrient protein food
With non-equal nitrogen nutrient protein food stomach-filling intervention.By movements in seven weeks and protein diversity intervention, carries out sports fatigue correlation and refer to
Mark detection.Experiments have shown that: the nutrient protein food of 1. equal nitrogen proportions of the invention can significantly improve lactic dehydrogenase after movement
The activity of enzyme.Nutrient protein food (waiting nitrogen) group lactic acid dehydrogenase activity is consistently higher than independent whey in 150 minutes after exercise
Albumen intervention group and soybean protein isolate intervention group.2. nutrient protein food (waiting nitrogen) of the invention can significantly extend once
Property swimming with a load attached to the body power exhausts the time.Nutrient protein food (waiting nitrogen) group rat negative G exhausted average time at 135 seconds, was significantly higher than cream
Albumin group (88 seconds average) and soybean protein isolate group (99.8 seconds average).3. nutrient protein food (waiting nitrogen) of the invention is eaten
Object will not obviously increase rat body weight.Rat lactalbumin, soybean protein isolate and nutrient protein food are fed by equivalent, seven
Zhou Housi group weight does not have obvious statistical difference.
Experiment shows that nutrient protein food of the invention acquisition its effect of food in the case where waiting nitrogen ratio is best, in equal nitrogen ratio
The just decline in various degree of attachment effect.It is just remarkably decreased deviateing 1.5% or more effect of parts by weight.
The present invention uses nutrient protein food (nitrogen is waited to match), passes through nutritional intervention after rat hydrostatic swimming in seven weeks, energy
It is enough that lactic acid dehydrogenase activity in rat body is significantly improved under the precursor for not increasing rat body weight, extend rat swimming with a load attached to the body power
Exhaust the time.Have effects that significantly to alleviate sports fatigue, so that rat swimming with a load attached to the body power is exhausted average time and reach 135 seconds, in anti-fortune
There is good utility value in dynamic fatigue function food exploitation.
Detailed description of the invention:
Disposable power exhausts time diagram after Fig. 1 lactalbumin, soybean protein isolate and feeding in nutrient protein food seven weeks
Specific embodiment: below by nutrient protein food (soybean and ox in the specific embodiment narration present invention
The nitrogen such as cream proportion) in anti-sports fatigue good result and application.Unless stated otherwise, technological means used in the present invention is
Method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, to be not intended to limit the present invention model
It encloses, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this hair
Under the premise of bright spirit and scope, double protein preparation in these embodiments and corresponding nutritional intervention scheme are carried out various
Change or change also belongs to protection scope of the present invention.
Embodiment 1:
The nutrient protein food of 100 kilograms of preparation measures raw material lactalbumin using Dumars azotometer and soybean separates
The N content of albumen is respectively NCreamAnd NBeans, according to mentioned above principle, that is, NCream=NBeansIt calculates needed for 100 kilograms of equal nitrogen double protein nutriments
Material content, with the N that measures of the present inventionSoybean protein isolate/ g=0.12 and NLactalbuminFor/g=0.10, it is double to prepare 100 kilograms of equal nitrogen
Nutrient protein food needs to weigh 45.4 kilograms of soybean protein isolates and 54.6 kilograms of lactalbumins.In 500 kilograms of profession mixing
It is separately added into 45.4 kilograms of soybean protein isolates and 54.6 kilograms of lactalbumins in device, 12 revs/min, mixing 15 minutes.
It is prepared as follows out fluid nutrient protein food as needed, it is Celsius to be added corresponding 50 in above-mentioned albumen powder
It spends pure water to be hydrated 30 minutes, prepares the equal nitrogen nutrient protein food liquid of various concentration.
1 example 1 of table
| Equal nitrogen nutrient protein food (kilogram) | Soybean protein isolate (kilogram) | Lactalbumin (kilogram) |
| 100 | 45.4 | 54.6 |
The preparation method of control experiment nutrient protein food:
The nutrient protein food of 100 kilograms of preparation, weighs 40 kilograms of soybean protein isolates and 60 kilograms of lactalbumins.?
40 kilograms of soybean protein isolates and 60 kilograms of lactalbumins, 12 revs/min, mixing are separately added into 500 kilograms of professional mixers
15 minutes.It is prepared as follows out fluid nutrient protein food as needed, it is Celsius to be added corresponding 50 in above-mentioned albumen powder
It spends pure water to be hydrated 30 minutes, prepares the nutrient protein food liquid of various concentration.
Embodiment 2
The nutrient protein food of 10 kilograms of preparation measures raw material lactalbumin and soybean separation protein using Dumars azotometer
White N content is respectively NCreamAnd NBeans, according to mentioned above principle, that is, NCream=NBeansIt calculates needed for 10 kilograms of equal nitrogen double protein nutriments
Material content, the N measured with the present inventionSoybean protein isolate/ g=0.12 and NLactalbuminFor/g=0.10,10 kilograms of equal nitrogen egg-pairs are prepared
White nutriment needs to weigh 4.54 kilograms of soybean protein isolates and 5.46 kilograms of lactalbumins.In 50 kilograms of mixers respectively
It is added 4.54 kilograms of soybean protein isolates and 5.46 kilograms of lactalbumins, 12 revs/min, mixing 15 minutes.Concentration as needed
The equal nitrogen albumen powder for weighing corresponding weight again is added corresponding 50 degrees Celsius of pure water and is hydrated 30 minutes, prepares the equal nitrogen of various concentration
Nutrient protein food liquid.
2 example 2 of table
| Equal nitrogen nutrient protein food (kilogram) | Soybean protein isolate (kilogram) | Lactalbumin (kilogram) |
| 10 | 4.54 | 5.46 |
Effect experiment technical solution of the present invention is as follows:
1, animal selects
6 SPF grades of week old male Wistar rats 24, are purchased from Beijing dimension experimental animal Co., Ltd, tonneau China, and experiment is dynamic
Object quality certification NO.11400700105348, production licence number: SCXK (capital) 2010-0001.Using IVC rat feeding system,
12h periodicity of illumination strictly observes Chinese animal practical guide.Weight 202-226g, is randomly divided into four groups: lactalbumin group, big
The nitrogen groups such as beans protein isolate group, nutrient protein food and the nitrogen group such as non-, motion model exhaust trip using the disposable power of wistar rat
Swimming model.
3, motion model
Swimming is derived from the domestic water for meeting sanitary standard with water, uses preceding carry out high temperature high pressure process.Depth of water control exists
Twice rat height.Water temperature selects to make it closer to normal rat body temperature between 30-35 DEG C.The side of sheet lead is wound by tail
Formula carries out weight loading exercise, and loading weight is the 10% of every rat body weight.The state for observing rat at any time during the motion, when
Still the judgment criteria of generation cannot be exhausted as power by making great efforts to return to the water surface after 10 seconds in its whole body immersion water.After the completion of movement,
Isothermal holding is carried out to rat as early as possible, is placed on after being wiped hair with towel in the mouse cage of dry padding.
4, testing program
Wistar rat is randomized into four groups, and is marked by ear tag.Firstly, carrying out by a definite date one to all rats
The adaptability feeding in week, second week test experience rat body weight, sheet lead weight needed for calculating swimming with a load attached to the body.It carries out on every Mondays primary
Property power exhaust training, Tuesday to Friday carries out the common swimming exercise that 2/3 power exhausts the time.Every time movement after respectively to four groups of rats into
Row stomach-filling processing (only, total concentration 200mg, every group carries out waiting nitrogen feeding stomach-filling amount 2ml/).Continuous feeding 6 weeks, the 7th week (week
One) stomach-filling intervention after exhaustive exercise, 30min, 60min, 90min, 120min and 150min are adopted after stomach-filling respectively
Collect tail vein.Blood physiology and biochemistry measures tired relevant parameter creatine kinase (CK) and lactic dehydrogenase (LDH).Furthermore it records
The different disposable power of all rats of intervention group exhaust the time within 7th week.
5, diet and drinking-water during testing
All wistar rats of participating in the experiment are using free water.The basic maintenance feed of diet use (quantitative feeding: 20g//
It) the uniform SPF grades of wistar rats of 6 week old male of weight are randomly divided into four groups i.e. lactalbumin group, soybean protein isolate
Group, nutrient protein food (waiting nitrogen) group, nutrient protein food (non-equal nitrogen) group, every group six.Ear is carried out to every group of rat respectively
Hereafter mark label carries out adaptability feeding in 1 week by a definite date, takes free water and quantitative feeding SPF rat to tie up during adaptability
Hold feed (20 gram/pcs/day).Carry out the swimming instruction of rat hydrostatic daily simultaneously.Second week starts to measure rat body weight, calculates big
Mouse hydrostatic swimming weight-bearing lead tare weight amount.A negative G is completed daily and exhausts swimming exercise, immediately respectively to every group of progress after movement
Albumen stomach-filling intervention.Stomach-filling amount is 2 milliliters, and concentration is 200 milligrams.Weekend rest maintains drinking-water and quantitative feeding rat to maintain
Feed.It records within 7th week every group of rat body weight (table 2) and power exhausts the time.Power carries out albumen stomach-filling after exhausting, and exists respectively after stomach-filling
30min, 60min, 90min, 120min and 150min carry out tail vein blood detection lactic acid dehydrogenase activity (Fig. 1).As a result such as
Under:
1, under the same terms, nutrient protein fed diets will not obviously increase rat body weight.
Different group the 7th week changes of weight situation of wistar rat of table 3
(every group of quantity=6, means standard deviation, gram)
The uniform SPF grade wistar rat of this experimental selection weight, every rat SPF grades of rats of daily quantitative feeding 20g
Maintain feed, free water.After swimming with a load attached to the body training, every group of rat takes in the protein (whey of equivalent by stomach-filling respectively
Albumen, soybean protein isolate, etc. nitrogen albumen and the nitrogen albumen such as non-).It is handled by hydrostatic swimming instructions in six weeks and albumen stomach-filling,
7th week detection each group rat body weight.Testing result shows that rat body weight does not have apparent statistics between each albumen intervention group
Difference (table 3), lactalbumin intervention group rat average weight are 360.83 grams, slightly above other three groups.
2, lactic acid dehydrogenase activity after rat motor is remarkably reinforced in nutrient protein fed diets.
The different group wistar rat lactic acid dehydrogenase activity dynamic changes of table 4
(every group of quantity=6, means standard deviation, U/L)
Lactic acid is the metabolite of internal glycogen oxygenolysis under anoxic conditions, and the accumulation of lactic acid is to lead to sports fatigue
One of substance.Lactic dehydrogenase is widely present in body tissue cell, can be catalyzed between lactic acid and pyruvic acid and be converted.Knot
Fruit shows, before swimming exercise, four groups of rat whey dehydrogenase activities are identical not to have statistical difference, after " movement+albumen " intervention
The nitrogen group such as 30min nutrient protein food lactic acid dehydrogenase activity is significantly raised, and other three groups of raising trend are consistent, significant low
In nutrient protein food (waiting nitrogen) group (P < 0.05).Then to 60min, nutrient protein food (waiting nitrogen) group lactic dehydrogenase
Activity drastically reduce to other three groups of consistent levels.From 60min to four groups of rat blood serum lactic dehydrogenases between 150min
Activity does not have notable difference, and 150min each group rat substantially returns to quiescent condition.
3, nutrient protein fed diets are obviously prolonged the disposable swimming time of rat (attached drawing 1)
According to the 7th Thursday group rat body weight numerical value, tail portion sheet lead weight is calculated according to the weight bearing principle of weight 10%, respectively
Swimming with a load attached to the body campaign is carried out to every group of rat.It records every rat and reaches the time that power exhausts state, lactalbumin group mean force exhausts
Time is 88s, and it is 99.8s, standard deviation 10.7, albumen battalion that standard deviation 21.7, soybean protein isolate group mean force, which exhaust the time,
Support the nitrogen group mean force such as food exhaust the time be 135.8s standard deviation be 16.2, nutrient protein food is non-etc., and nitrogen group mean force exhausts the time
105.7s standard deviation 15.4.The non-equal nitrogen group group rat of statistical analysis nutrient protein food reaches the product equal time that power exhausts state
It is significantly higher than other three groups (P < 0.05), mean force exhausts the time and do not count between lactalbumin group and soybean protein isolate group
Learn difference.
Claims (7)
1. a kind of protein food, is made of soybean protein isolate and lactalbumin, in the protein food soybean protein isolate and
The parts by weight of lactalbumin are as follows: 45.4 parts of soybean protein isolate, 54.6 parts of lactalbumin, which is characterized in that
The soybean protein isolate preparation process is as follows:
Distilled water mixing is added in soybean meal, and mixed proportion is+90% distilled water of 10% dregs of beans, and NaOH is added after mixing, adjusts
PH=7, solution are centrifuged, centrifugal speed 3800rpm, centrifugation time 30min；Supernatant is taken, hydrochloric acid is added into supernatant
PH to 4.5 is adjusted, after the protein such as standing are precipitated completely, 3800rpm is centrifuged 30min, abandons supernatant, takes precipitating；Into sediment
NaOH, which is added, makes pH=7, by being dried by spraying；
The lactalbumin preparation process is as follows: using 25% dehydrated alcohol extract cow's milk in crude protein, by gained filtrate according to
Dehydrated alcohol is added in 2.8:1 ratio, after standing 30min, lactalbumin is obtained with 4000rpm centrifugation 30min, by clarifying, surpassing
Filter, dry acquisition whey protein concentrate.
2. it is each to add the preparation of food auxiliary material on the basis of the protein food for a kind of protein food according to claim 1
Kind food product.
3. a kind of protein food according to claim 1,100 kilograms of protein foods, 45.4 kilograms of soybean protein isolate, whey
54.6 kilograms of albumen.
4. a kind of protein food according to claim 1,10 kilograms of protein foods, 4.54 kilograms of soybean protein isolate, whey
5.46 kilograms of albumen.
5. a kind of preparation method of protein food according to claim 1, specifically includes:
It weighs 45.4 parts of soybean protein isolate respectively according to parts by weight, 54.6 parts of lactalbumin, is mixed to prepare protein food.
6. a kind of preparation method that fluid albumen food is made according to any protein food of claim 1-4, soybean separation
50 DEG C of warm water 300rpm is added after albumen and lactalbumin mixing adds water to mix 30 minutes, is made final concentration of 100mg/ml's
Fluid nutrient protein food.
7. application of a kind of protein food in terms of preparing the antifatigue food of animal according to claim 1.
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