CN105601405A - Microbial targeting flora liquid organic fertilizer - Google Patents
Microbial targeting flora liquid organic fertilizer Download PDFInfo
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- CN105601405A CN105601405A CN201510690986.4A CN201510690986A CN105601405A CN 105601405 A CN105601405 A CN 105601405A CN 201510690986 A CN201510690986 A CN 201510690986A CN 105601405 A CN105601405 A CN 105601405A
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Abstract
The invention belongs to a microbial targeting flora application technology, and especially relates to a microbial targeting flora liquid organic fertilizer. A basic raw material formula comprises: a 50% d1 organic fertilizer as a main material, wherein the main material comprises 20% of organic farm manure, 10% of organic waste residues and 20% of organic crop straws; a 25% flora medium raw material used as an auxiliary material, wherein the auxiliary material comprises 2% of beef extract, 5% of (juiced) soybeans, 7% of syrup, 5% of a bamboo juice and 6% of starch; and 25% microbial targeting florae and an additive as a function synergist, wherein the function synergist comprises 4% of (first generation) Bacillus subtilis with the quantity of 100 billions/g, 4% of lactic acid bacteria, 4% of yeast original inoculum, 5% of Trichoderma spp., 4% of polyaspartic acid (polypeptide active enzyme) and 4% of a biological enzyme activator. The microbial targeting flora liquid organic fertilizer is prepared through crushing, sieving, concentrated stacking, synthesis fermentation and air drying.
Description
Technical field
The invention belongs to microorganism target flora application technology, relate in particular to a kind of microorganism target flora liquidFertilizer.
The meaning of the target that the present invention locates, refers to by between the different biological inoculums in the biological Pseudomonas of differenceInterpromotion and interrestraint relation and function orientation. Choose, optimize cultivation, flora combination, effect target calmly by bacterial classificationThe problem concept that the thoughtcast of position is established.
Technical background
Along with globalization extensive use of chemical fertilizer, the impact of agricultural chemicals main trend, the use amount of world Fertilizer is by 1400 of nineteen fiftyTen thousand tons are increased to 100,000,000 2 thousand 6 hundred ten thousand tons in 1984. In the meantime, also increasing every year. This artificially contraryVicious circle, not only cause the deterioration of the ecological environment such as soil, water body, agricultural quality decline. SimultaneouslyAlso bring serious threat also to human survival health.
According to statistics, the whole world has 2,100 ten thousand hectares of fertile arable lands to lose production capacity every year, sends out by this trendExhibition is gone down, and to the end of this century, the arable area in the whole world will reduce 1/3. To ecological environment, health bandCome, with the serious impact that becomes, to have caused people's great attention. The total cultivated area of China drops to1800000000 mu, 1.4 mu of less thaies per capita, have the per capita cultivated land of 664 cities and counties the definite per capita cultivated land of the United NationsBelow the warning line of 0.8 mu. Realize foodgrain self-sufficiency self-sustaining, ensure the fundamental state policy of staple food supply as China,All previous governments all attach great importance to. In order to meet the demand of people to grain and agricultural byproducts, must increase grainOutput is the effective means that improves grain yield and apply chemical fertilizer. At present traditional fertilizer as: nitrate fertilizer,Ammoniacal nitrogen fertilizer, amide nitrogen fertilizer, cyanamide nitrogen fertilizer etc., all have certain effect of increasing production.
Conventionally, use the chemical fertilizer of a ton, can increase the grain yield of 9 tons. But after 1984,The anxious of fertilizer application amount increases, the increase that does not but bring grain yield, and contrary soil has but been subject in various degreeDestruction. Such as, in some developed areas of China, nitrogen application is obviously excessive, causes the nitre of soilState nitrogen accumulates in a large number, thereby easily causes accumulation and the conversion of nitrate at food chain. Copper, zinc, manganese etc. are micro-Secondary element is being served as important role in plant, animal and human's vital functions, lacks this dvielement meetingCause physiological function obstacle and cause corresponding disease. Thus, develop with the good environment of sacrificing soilEconomy is a kind of harmful acts and efforts for expediency, be subject in the end the Nature be further intensified revenge or our mankind,Therefore find and prevent that soil pollution countermeasure from being agriculturist's obligation one can't decline.
The sudden emergence of micro-ecological agriculture, has not only reflected that the mankind are to back to nature, U.S. for protection of the environmentGood hope. , also can man's lot to change meanwhile, bring new life.
Summary of the invention
Bring serious threat for improving above-mentioned present situation, the present invention is agricultural and organic using the ecological agriculture as industrializationAgricultural combination, is mainly conceived to the dynamic equilibrium of whole Agro-ecological System.
The object of the invention is: micro-for solving the ecocrisis of facing mankind and one that commercial articles safety providesBiological targeting flora liquid organic fertilizer. The present invention has improvement soil, guarantees that soil is balanced in nutrition, improves agricultureThe quality of crop, increases output, pollution-free, is conducive to the effect of environmental protection.
Original effect of the present invention:
Not only there is improvement soil, improve soil organic matter of cultivated land, effectively prevent and treat diseases and pests of agronomic crop, special agricultural productionThe organic quality of product, strengthen fruit and vegetable food mouthfeel, extend the shelf life, reduce the polynary effect of agricultural product input costShould. Meanwhile, also can form to transgenic product market the challenge of contrast property, thoroughly allow China and even the world eachGround, is completely free of and violates the rules of nature and carry out the dependence of genetically modified crops.
Can also serve as soil conditioner, the feasible greenhouse vegetable planting soil salinization of soil feelings of effectively having controlledCondition and improved soil fertility. After using, can increase soil organic matter content, ECE, available nutrient,The miniature organism amount of the voidage of soil and permeability and soil, has obviously improved soil fertility. Itself and undergroundThe method of closed drainage combination can be controlled greenhouse soil salinization of soil most effectively, and farm manure and chemical fertilizer improve relativelyThe yield and quality of vegetables.
As soil sterilants, can pollute the soil of damage and the soil that environmental disruption causes to industrialness simultaneouslyWith use chemical fertilizer and agricultural chemicals and excessively cause the soil hardening, take sedimentation etc. to carry out soil remediation, completely canEffect that cost is low to reach, instant effect, control are held concurrently mutually.
The present invention has: production cost is low, cost of equipment is low, technique is simple, fabrication cycle is short, both canTo carry out industrialization production, can walk close to again huge numbers of families' feature.
Nonhazardous: use for a long time this product, also noresidue is poisoned. Share and can remove in contaminated soil with solidAgriculture is residual, medicine is residual;
Many effects: effectively phosphorus decomposing, potassium decomposing, increase photosynthesis, keep soil from packing together; Can eliminate in paddy fieldBlue-green algae green alga and water duckweed, prevent that itself and crop from striving fertilizer; The prosperity of crops root system, stalk is sturdy, volume increaseAmplitude is large.
The accelerating phase: the present invention can make crops maturation shift to an earlier date 10%.
Technical scheme is by the original technology of the physical property optimum organization of science, functional effect target location. FromMore than 10 Pseudomonas such as photosynthetic bacteria, actinomyces, saccharomycete, lactic acid bacteria in kind, chosen wherein more than 90:Lactic acid bacteria (Lactobacillus), yeast original seed bacterium, Trichoderma, bacillus subtilis 5% (generation), 1000Individual hundred million/gram upper, biological enzyme activator, poly-aspartate (poly-peptide organized enzyme) are target effect flora. Pass through againThe high-tech means such as breeding strain, target combination, fermentation increment, compound cultivation are made.
The present invention requires the Essential Environment feature possessing to be:
Microenvironment: the microenvironment of microorganism refers to directly to affect microorganism survival and development, with the pass of microorganismIt is the surrounding environment of the closest microbial cell;
Stability: due to the diversity of microbe species in microbial ecosystem, make it in environmental condition oneWhile determining the variation of scope, the characteristic of microbe species composition C.T;
Adaptability: when the microenvironment generation acute variation of microorganism, the structure of community of microorganism can occur correspondingThe characteristic of variation.
Growth of microorganism rule and breeding method that the present invention follows are:
The growth rhythm of micropopulation: adjustment period, logarithmic phase, stationary phase and decline phase;
Certain a small amount of microbial bacteria is inoculated on the fluid nutrient medium of constant container, and is positioned over suitable barUnder part, cultivate the micropopulation growing state in period sampling measuring culture medium;
Step is: inoculation---manually cultivates---regularly sampling---and measures growth.
The environmental factor that the present invention focuses on is:
Temperature: growth of microorganism the temperature in vigorous period optimum growth temperature;
PH value: after exceeding optimum PH scope, can affect activity, the stability of cell membrane etc. of enzyme, fromAnd affect the absorption of microorganism to nutriment etc.; The optimum PH of most microbial bacterials is 6.5-7.5,Fungi optimum PH is 5.0-6.0;
Oxygen: the situation of oxygen content in environment, the growth of the micropopulation to different metabolic type, has notSame impact. Requirement according to microorganism to oxygen content, can be divided into: aerobic type, anaerobic type and amphimicrobianMicroorganism.
Concrete formula of the present invention and target be quantitatively:
Bacteria group culture based raw material 30%:
Wherein: syrup: 20% (being formed by white granulated sugar 5%, 15% modulation of sugared slag); Soybean: 10%.
Target flora and additive 30%:
Wherein: lactic acid bacteria (Lactobacillus) 5%; Yeast original seed bacterium 5%; Trichoderma 5%; Withered grass budOn spore bacillus 5% (generation), 1000 hundred million/grams; Biological enzyme activator 5%; Poly-aspartate (poly-peptide activityEnzyme): 5%.
Water 40% (being advisable with 42-45 degree).
Flora in formula material of the present invention and the target function of additive:
(1) bacillus subtilis (Bacillussubtilis); Bacillus subtilis thalline is in growth courseThe subtilin of middle generation, polymyxins, nystatin, gramicidins isoreactivity material have bright to pathogenic bacteriaAobvious inhibitory action, can promote the growth of plant, has increased plant height and leaf area, has improved plant SOD, PODWith the activity of CAT protective enzyme, reduce the content of MDA, activating soil, prevents from hardening, and improves soil originallyThe fertilizer efficiency of body, improves the output of crops and the quality of fruit.
(2) lactic acid bacteria (Lactobacillus); Bifidobacterium, can suppress the breeding of harmful bacteria, can be highEffect decomposing organic matter, degradation of ammonia nitrogen, nitrite and heavy metal ion, lactic acid bacteria can pass through at short noticeBreeding produces metabolic active substance, and available amino acid (as lysine and methionine) is provided, organic acid,Hydrogen peroxide, carbon dioxide etc. and various vitamin (vitamin B complex and K, H etc.), can also produce bacteriocin,As: nisalpin, lactein, bites sour rhzomorph etc. Suppress harmful bacteria breeding, make fertilizer produce slant acidity,Improve overall fertile matter. Lactic acid bacteria is the activating agent of all biologies, plant, to vegetables, fruit and cereal etc.Growing plays important active function, and accelerates the fermentation of compost. And to deodorizing and soil improvement alsoThere is remarkable result.
(3) Trichoderma: trichoderma, in filamentous fungi class, has antagonism for various plants pathogen,Especially native transmissibility fungi, for example reaping hook germ (Fusariumspp.), rotten mildew fungus (Pythiumspp.),Southern blight bacterium (Sclerotiumrolfsii) and Rhizoctonia solani Kuhn (Rhizoctoniasolani) etc., often applicationIn biological control. Trichoderma mainly contains antibiosis for the antagonism of phytopathogen, super parasitism and competing life etc.Effect. Because Trichoderma mycelial growth is quick, strong for stress tolerance, be conducive to the competition of soil environmentAnd the foundation of group, also can produce antibiotics, as gliotoxin and gliovirin, there is anti-system rotten mouldThe effect of germ Pythiumultimum. Its super parasitics can make it parasitize the thalline of disease fungusOn cause phytopathogen death, on Rhizoctonia solani Kuhn, cause the contracting of hanging of its thalline dead as Trichoderma can parasitizeDie.
(4) saccharomycete: most yeast can be located away from the environment that is rich in carbohydrate, such as some fruit (PortugalsGrape, apple, peach etc.) or plant secretion thing (as the juice of cactus). Some yeast are raw in insect bodiesLive. Saccharomycete is unicellular eukaryotic microorganisms. Saccharomycete has typical eucaryotic cell structures, have cell membrane,Cell membrane, nucleus, cytoplasm, vacuole, mitochondria etc., what have also has a microbody.
Yeast is divided into two kinds, yeast cake, dry ferment, is a kind of edible, nutritious unicellular micro-lifeThing, in nutrition is called it " inexhaustible nutrient source ". Except protein, carbohydrate, fatBeyond class, yeast is also rich in multivitamin, mineral matter and enzyme. Experiment showed, every 1 kilogram of dry fermentFemale contained protein, is equivalent to the protein content of 5 kilograms of rice, 2 kilograms of soybean or 2.5 kilograms of porks.Therefore, nutritional labeling contained in steamed bun, bread will exceed 3-4 doubly than large flat bread, noodles, and protein increasesNearly 2 times.
Yeast after fermentation or a kind of very strong polyphenoils, can protect liver, has certain removing toxic substances to doWith. The mineral energy such as selenium, chromium in yeast is anti-ageing, antitumor, prevention of arterial sclerosis, and improves human bodyImmunity. After fermentation, in flour, a kind of phytic acid that affects the element absorption such as calcium, magnesium, iron can be decomposed,Thereby improve absorption and the utilization of human body to these nutriments.
Yeast produces a large amount of carbon dioxide in dough fermentation, and due to the formation of gluten network tissue, andBe left in desmachyme, make baked goods tissue looseness porous, volume increases.
Yeast increases the effect of gluten expansion in addition, and the carbon dioxide producing while making to ferment can be retained in doughIn, the gas ability of holding of raising dough. As with chemical raising agent without this effect.
Because the main component of yeast is protein, almost account for the half content of yeast dry matter, Er QierenThe lysine content lacking in body essential amino acids content abundance, especially cereal is more. On the other hand,Contain a large amount of vitamin B1s, vitamin B2 and niacin. So yeast can improve the nutrition of fermented foodBe worth.
The saccharomycete of unicellular eukaryote has more complete gene expression regulation mechanism and to expression productProcessing Modifying Capability. Saccharomyces cerevisiae (Saccharomyces.Cerevisiae) is aspect molecular geneticsBy people's understanding the earliest, be also the yeast host as exogenous gene expression at first.
Saccharomycete with other live organism equally need similar nutriment, bacteria-like the same it have a set ofIn born of the same parents and ectoenzyme system, the little molecule thing easily utilizing in order to macromolecular substances is resolved into cell metabolismMatter belongs to heterotrophism.
As bacterium, saccharomycete must have water to survive, but the moisture that yeast needs is fewer than bacterium, certainA little yeast can be grown in the few environment of moisture, and as honey and jam, this shows that they are pressed with phase to infiltrationWhen high tolerance.
Saccharomycete can be grown in the scope that be 3.0-7.5 in pH value, and optimum pH is pH4.5-5.0.In the freezing point lower than water or at higher than the temperature of 47 DEG C, yeast cells generally can not be grown, the most suitable growth temperatureDegree is generally at 20 DEG C-30 DEG C.
Saccharomycete can be grown in the environment of aerobic and anaerobic, and saccharomycete is facultative anaerobe, at aerobicSituation under, it breaks sugar into carbon dioxide and water, in the time that aerobic exists, Yeast Growth is very fast. ?In the situation of anoxic, saccharomycete breaks sugar into alcohol and carbon dioxide. The yeast that purposes is the most often mentioned is madeBrewer yeast (also claiming Saccharomyces cerevisiae) (Saccharomycescerevisiae), since prehuman in several thousand justWith its fermentation bread and drinks, in the process of sour ferment bread and steamed bun, in dough, can emit carbon dioxide.Because yeast belongs to simple unicellular eukaryote, be easy to cultivate, and growth rapidly, is widely used in the modern timesIn biological study. If saccharomyces cerevisiae is as important model organism, be also science of heredity and molecular biologicalImportant research material. In saccharomycete, contain cyclic DNA---plasmid, can be used for engineered carrier.
(5) biological enzyme activator: be on the basis of biology enzyme phosphorus decomposing bacterium, added and promoted the work that phosphorus decomposesOrganic matter and the efficient activator produced activates multiple enzyme, promotes the synthetic multiple antibacterial material of crop,Significantly strengthen crop resistance; Fully meet multiple element demand, nutrient balance is reasonable, and fertilizer efficiency is surely long, adjustsJoint Stoma of Leaves is opened a business, and keeps moisture content, promotes root growth and vigor, resists arid and hot dry wind; ImproveChlorophyll content in leaf blades, stimulates photosynthesis, strengthens the anti-adversity abilities such as cold-resistant, freeze proof, impels precocity; SwashSoil beneficial microbe alive, the nutrients such as activation unavailable phosphorus, iron, calcium, magnesium, improvement soil physico-chemical property;In promotion, acid agrichemical dissolve, Synergistic slow release, reduce toxicity, reduce consumption, improve chemistry security.Stimulate rapidly and make synthetic multiple enzyme in object, impel crop root more flourishing, nutrient absorption is more sufficient, leafSheet photosynthesis significantly strengthens, and effectively improves the speed of growth of trees, and the speed that treelet grows into forest is significantly accelerated.
Basic equipment required for the present invention mainly comprises: first class seed pot, secondary seed tank, fermentation tank, batchingTank, bottle placer, air compressor machine, cold-producing medium, zymotic fluid storage tank, automatic filling machine, pump, filter, squeeze the juiceMachine.
Specific embodiments
Concrete implementation step and the technological process of microorganism target flora liquid organic fertilizer are as follows:
1, preferred → target flora combination that flora is trained grow → bacterial classification → by bacillus subtilis (generation), 1000Individual hundred million/gram upper 5%; Lactic acid bacteria (Lactobacillus) 5%; Yeast original seed bacterium 5%; Trichoderma 5% and poly-skyWinter propylhomoserin (poly-peptide organized enzyme) 5%; Biological enzyme activator 5%; After the science of carrying out compatibility, putting into seeding tank cultivates.
2, culture medium raw material preparation: first by 30% culture medium raw material in products material: syrup 20% (white granulated sugar5%, sugared slag 15%), soybean 10% use juice extractor is synthetic squeezes the juice → after filtering with filter, put into fermentation tank.
3, water distribution: when culture medium raw material in the time that fermentation cylinder for fermentation is spent to 42-45 → put into products material(42-45 degree) special reactive hydrogen water of 40% → add water is simultaneously with sending oxygen machine to send oxygen → put into 30% target floraPreparation → target flora carries out in the fermentation cultivation → tank of 20 days temperature and exceedes 60 and use while spending in fermentation tankThe temperature adjustment of cold-producing medium refrigeration.
4, product storage: the qualified microorganism target flora fertilizer stoste of detection is put into holding vessel and protectMatter stores (common 18 months of storage period).
5, detect pH value 3.5-7.5.
6, product is filling: qualified products are filling with bottle placer.
7, sizing packaging: common every bottle: 1 kilogram, every case: 10 kilograms.
8, using method:
Foliage spray: various crops (containing various fruit and vegetables): blade face after the dilution of 1: 400 times, the former source of bacterial manureSpray.
Crop fertilization: in the anti-blue or green phase of crop, growth period, each punching was executed once, common 5000 milliliters every mu.
Claims (5)
1. a microorganism target flora liquid organic fertilizer, is characterized in that, is made up of three parts, comprises targetTo flora and additive 30%, bacteria group culture based raw material 30%, water 40%.
2. according to a kind of microorganism target flora liquid organic fertilizer described in claims 1, it is characterized in that,Described target flora and additive 30%, comprise bacillus subtilis (generation), 1000 hundred million/grams upper 5%;Lactic acid bacteria (Lactobacillus) 5%; Yeast original seed bacterium 5%; (poly-peptide is lived for Trichoderma 5% and poly-aspartateProperty enzyme): 5%; Biological enzyme activator 5%; After the science of carrying out compatibility, putting into seeding tank cultivates.
3. according to a kind of microorganism target flora liquid organic fertilizer described in claims 1, it is characterized in that,Described bacteria group culture based raw material 30%, comprises syrup: 20%, and soybean: 10%, squeeze the juice with juice extractor is synthetic.
4. according to a kind of microorganism target flora liquid organic fertilizer described in claims 3, it is characterized in that,Described syrup: 20%, comprise white granulated sugar 5%, sugared slag 15%.
5. according to a kind of microorganism target flora liquid organic fertilizer described in claims 1, it is characterized in thatComprise following preparation technology's flow process:
Flora training is grown: bacterial classification preferably, the combination of target flora, carry out putting into seeding tank after science compatibility and cultivate,By 30% culture medium raw material in products material, after filtering with filter, put into fermentation tank;
Water distribution: when culture medium raw material when fermentation cylinder for fermentation is spent to 42-45, put into products material 40%(42-45 degree) special reactive hydrogen water, add water simultaneously with sending oxygen machine to send oxygen, putting into 30% target flora preparation,The fermentation that target flora carries out 20 days in fermentation tank is cultivated, and temperature exceedes 60 and uses cold-producing medium system while spending in tankCool tone temperature;
Product storage: put into the holding vessel storage of guaranteeing the quality by detecting qualified microorganism target flora fertilizer stosteDeposit (common 18 months of storage period);
Detect pH value 3.5-7.5;
Product is filling: qualified products are filling with bottle placer;
Using method: foliage spray: various crops (containing various fruit and vegetables): 1: 400 times, the former source of bacterial manureFoliage spray after dilution; Crop fertilization: execute once common every mu 5000 in anti-blue or green phase of crop, each punching in growth periodMilliliter.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111289756A (en) * | 2018-12-10 | 2020-06-16 | 北京师范大学 | Urine marker related to lung metastasis and tumor formation of ovarian cancer |
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| CN102964178A (en) * | 2012-11-13 | 2013-03-13 | 湖南泰谷生物科技股份有限公司 | Composite microbial bactericide, and preparation method and application thereof |
| CN103193543A (en) * | 2013-04-03 | 2013-07-10 | 郭同洲 | Pollution-free biological organic bacteria fertilizer with nutrient balancing function |
| CN103724126A (en) * | 2013-12-15 | 2014-04-16 | 张彩年 | Multielement high-efficiency active organic liquid fertilizer and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101774853A (en) * | 2010-01-07 | 2010-07-14 | 杨雪枣 | Antibacterial type composite biological organic liquid fertilizer and preparation method thereof |
| CN102964178A (en) * | 2012-11-13 | 2013-03-13 | 湖南泰谷生物科技股份有限公司 | Composite microbial bactericide, and preparation method and application thereof |
| CN103193543A (en) * | 2013-04-03 | 2013-07-10 | 郭同洲 | Pollution-free biological organic bacteria fertilizer with nutrient balancing function |
| CN103724126A (en) * | 2013-12-15 | 2014-04-16 | 张彩年 | Multielement high-efficiency active organic liquid fertilizer and preparation method thereof |
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| CN111289756A (en) * | 2018-12-10 | 2020-06-16 | 北京师范大学 | Urine marker related to lung metastasis and tumor formation of ovarian cancer |
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