CN105596432A - Application of jasminum cinnamomifolium kobuski extract in preparation of medicine resisting free radical oxidation damage of cardiac muscle cells - Google Patents
Application of jasminum cinnamomifolium kobuski extract in preparation of medicine resisting free radical oxidation damage of cardiac muscle cells Download PDFInfo
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- CN105596432A CN105596432A CN201610075451.0A CN201610075451A CN105596432A CN 105596432 A CN105596432 A CN 105596432A CN 201610075451 A CN201610075451 A CN 201610075451A CN 105596432 A CN105596432 A CN 105596432A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses an application of a jasminum cinnamomifolium kobuski extract in preparation of a medicine resisting free radical oxidation damage of cardiac muscle cells. The jasminum cinnamomifolium kobuski extract serving as an active ingredient can improve the T wave of the ST segment of a patient suffering from acute myocardial ischemia and also can resist the free radical oxidation damage of cardiac muscle cells and enhance the energy supply of the body so as to achieve an aim of treating myocardial ischemia.
Description
The application is the divisional application of the application for a patent for invention that proposes of applicant Wang Xueyan (denomination of invention is: a kind of medical composition and its use resisting myocardial ischemia, application number is: 2014103651409, the applying date is: on July 29th, 2014).
Technical field
The invention belongs to Chinese medicine extractive technique field, the purposes in particular to a kind of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury.
Background technology
Myocardial ischemia refers to that the hemoperfusion of heart reduces, and causes the oxygen supply of heart to reduce, and energy metabolism of myocardial is undesired, can not support a kind of pathological state of the normal work of heart. Interrelated data demonstration, myocardial ischemia mostly occurred after 40 years old, and average illness rate is about 6.49%. Along with the raising of living standards of the people, myocardial ischemia is the trend rising year by year in the illness rate of China at present, and morbidity crowd rejuvenation trend is obvious. Myocardial ischemia is also one of disease kind that the death rate is the highest, is common in acute sudden death, myocardial infarction etc. Modern medicine is thought, the common reason of myocardial ischemia is coronary atherosclerosis, secondly also has inflammation (rheumatic, syphilis, Kawasaki disease and thromboangitis obliterans etc.), spasm, embolism, connective tissue disease, wound and congenital abnormality etc. multiple.
Doctor trained in Western medicine has medicine treatment to this sick methods for the treatment of, and as antiplatelet drug, receptor blocking agent, calcium ion antagonist, statins, nitric acid lipid drug, thrombolysis class medicine etc., its curative effect is all not ideal, only only alleviate, and side effect is larger. Take stopgap measures and be difficult to effect a permanent cure in view of western medical treatment is limited to, even if comparatively winner of PCI and operative treatment, be also difficult to multiple to patient with healthy physique, the traditional Chinese medicine of therefore finding many target treatments seems particularly urgent.
Spun gold Calamus is in climbing shrub, high 1-4m. Complete stool unit hair. Cylindrical or the tool rill of sprig, footpath 1-2mm. Leaf is to life, Dan Ye; The long 4-10mm of petiole, reverses, and has joint; Blade papery or thin keratin, oval or narrow ellipse, rare by aciculiform, long 5-10.5cm, wide 1.5-4.5cm, the sharp point of tip is point extremely gradually, base portion wedge shape or circle, leaf margin warp, base goes out 5, arteries and veins, and 1 pair, outside is not obvious, upwards extends through top, and is connected with transverse vein. Flower Dan Sheng, or be umbrella cyme, top is raw or armpit is raw, has colored 1-5 piece; Inflorescence is without stalk or the long 0.2-2cm of stalk; Bract is linear, long 2-4mm; Bennet is elongated, and long 1.4-2.5cm is upwards cumulative thick; Calyx pipe range 2-3mm, 5 pieces of slivers, sharp triangle, long 1-2mm; Corolla white, high pin dish shape, corolla pipe range 0.9-1.3cm, footpath 1-2mm, sliver 9-11 piece, lanceolar, long 1.1-2cm, wide 1.5-3mm, tip is point gradually. Fruit is spherical or oval, long 1-1.5cm, and footpath 0.8-1.5cm, is black. The florescence 3-9 month, the fruit phase 5-11 month. Be born in woods or sand ground. Be distributed in Hainan, Yunnan.
At present, spun gold rattan is in the news and has clearing heat and detoxicating, synthetism is cured the wound effect as Chinese medicine, be mainly used in controlling abscess of throat, pathopyretic ulcer, fracture and traumatism and bleeding, there is no bibliographical information spun gold rattan or its extract and there is the biologically active that strengthens acute myocardial ischemia patient cardiac muscle energy supply or anti-myocardial and be subject to Free radicals injury.
Summary of the invention
The object of the invention is to by secret prescription handed down in the family from generation to generation is furtherd investigate, thereby a kind of pharmaceutical composition resisting myocardial ischemia is provided, a kind of pharmaceutical composition of anti-acute myocardial ischemia is especially provided. This spun gold boisiana extract can be developed into the pharmaceutical preparation of the disease due to the myocardial ischemias such as treatment coronary heart diseases and angina pectoris.
Particularly, the object of the invention is to adopt following technical scheme to realize:
The pharmaceutical composition resisting myocardial ischemia, is prepared from by acceptable auxiliary material in active component and pharmacy, it is characterized in that, described active component comprises spun gold boisiana extract.
Preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, described spun gold boisiana extract is prepared from by the following method: get spun gold rattan raw material, add aqueous sodium carbonate refluxing extraction, extract regulates pH=2.8-3.2 with hydrochloric acid, leave standstill, filter, get precipitation and be dried, obtain spun gold boisiana extract.
Further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, the concentration of described aqueous sodium carbonate is 3%-10%.
Again further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, the concentration of described aqueous sodium carbonate is 4%-7%.
Again further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, the 8-15 that the consumption of described aqueous sodium carbonate is raw material weight is doubly.
Further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, the number of times of described refluxing extraction is 1-4 time, boils 2-4h at every turn.
Further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, described extract regulates pH=3.0 by acid solution.
Further preferably, the pharmaceutical composition resisting myocardial ischemia as mentioned above, wherein, the time of described standing step is 4-24h.
It should be noted that the tender leaf of the spun gold rattan raw material choose Oleaceae plants leaf of cinnamomi Jasmine JasminumcinnamomifoliumKobuski that the present invention adopts in the time of the described spun gold boisiana extract of preparation. Can select the dry product drying, also can select fresh wet product.
The inventor causes that by lumbar injection Pit coronarospasm is to copy Model Rats with Acute Myocardial Ischemia. Pit extracts the material obtaining from animal pituitary, contain pitressin (antidiuretin) and oxytocins, pitressin can directly make blood vessel, particularly capillary and parteriole shrink, thereby can make coronarospasm, contraction make myocardial ischemia-anoxemia. Therefore, the Model Rats with Acute Myocardial Ischemia that the present invention copies occurs that abnormal electrocardiographic pattern changes, and is mainly reflected in ST section, T ripple is abnormal. Carry out therapeutic intervention discovery by giving spun gold boisiana extract, spun gold boisiana extract can improve rat model electrocardiographic abnormality situation, to play protection Myocardial Effects. In addition, in acute myocardial ischemia process, ultra-oxygen anion free radical (O2 -) a large amount of formation and the increase of lipid peroxidation be one of main pathologic process of cellular damage. SOD is extensively present in organism and the closely-related protein of cell oxygen metabolism, highly in specific manner the catalysis O harmful to body2 -Disproportionated reaction, and get rid of O2 -To the infringement of body cell, tissue, the damage of antagonism ischemic hypoxia disease, hypoxic-ischemic again perfused tissue damage have important protective effect. MDA is the stable metabolin of peroxidatic reaction of lipid, measure MDA content can reflecting myocardium tissue in O2 -Content and the peroxidatic reaction of lipid degree of initiation, indirectly reflect that cell is subject to the degree of Free radicals injury. The present invention shows by above-mentioned experimental study, and spun gold boisiana extract can significantly strengthen model rat heart muscle SOD activity, reduces MDA content, thus protection damaged myocardium. Moreover ATP is a kind of coenzyme, participate in the metabolism such as body protein, fat, sugar, nucleic acid, nucleotides, be again the main source of human body energy, its metabolic disorder can reflect that body energy supply is abnormal. The present invention shows by above-mentioned experimental study, and spun gold boisiana extract can significantly strengthen model rat heart muscle ATP content, thereby ensures the energy supply abundance of damaged myocardium. In sum, spun gold boisiana extract can play a protective role to Model of Acute Myocardial Ischemia rat, and it is machine-processed and improve heart ST section, T ripple and anti-oxidant, strengthens body energy supply relevant.
Based on above-mentioned result of study, another object of the present invention is to provide a kind of pharmaceutical applications, that is: the purposes in the medicine that above-mentioned spun gold boisiana extract resists myocardial ischemia in preparation. Or: above-mentioned spun gold boisiana extract improves the purposes in the medicine of acute myocardial ischemia patient electrocardiographic abnormality in preparation; The purposes of above-mentioned spun gold boisiana extract in the medicine of preparation enhancing acute myocardial ischemia patient cardiac muscle energy supply; The purposes of above-mentioned spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury.
Compared with prior art, the pharmaceutical composition the present invention relates to is prepared from as unique raw material by spun gold rattan, contained spun gold boisiana extract can improve acute myocardial ischemia patient's heart ST section, T ripple as active component, can be subject to Free radicals injury by anti-myocardial simultaneously, strengthen body energy supply, thereby reach the therapeutic purposes of the myocardial ischemia such as resisting coronary heart disease, angina pectoris disease.
Detailed description of the invention
Be below Preparation Example and the effect test example of spun gold boisiana extract of the present invention, the present invention will be further described and explanation. It should be noted that the dry tender leaf of the spun gold rattan raw material choose Oleaceae plants leaf of cinnamomi Jasmine JasminumcinnamomifoliumKobuski that following examples adopt in the time of the described spun gold boisiana extract of preparation.
The preparation of embodiment 1 spun gold boisiana extract
Take dry spun gold rattan 850g, impurity elimination, adds 10L mass percentage concentration and is 5% sodium carbonate liquor, soak 40min, refluxing extraction 2 times is boiled 3h at every turn, filter, merging filtrate, regulates filtrate pH=3.0 with concentrated hydrochloric acid, normal temperature hold over night, filters, and gets precipitation, dry, pulverize, cross 100 mesh sieves, obtain spun gold boisiana extract dry powder.
The affect experimental study of embodiment 2 spun gold boisiana extracts on Model of Acute Myocardial Ischemia rat
60 of clean level SD rats, male, body weight 150～180g, experiment is divided into following five groups, be Normal group (wait and hold physiological saline), model control group (wait and hold physiological saline), ISDN (15mg/kg) and spun gold boisiana extract high and low dose (spun gold boisiana extract dry powder 180,60mg/kg prepared by embodiment 1) group, 12 every group. Gastric infusion, every day 1 time, 15d continuously, 1h after last administration, except Normal group, all the other respectively organize lumbar injection Pit (20U/kg) to copy Acute Myocardial Ischemia Rats model.
After last administration 1h, lumbar injection 3% yellow Jackets (1mL/kg) anesthesia rats in normal control group, back of the body position is fixing, and the needle electrode of polygraph inserts respectively subcutaneous, and first Continuous Observation also records one section of normal ECG; After reconstructed model, detect all the other each group Rat Ecg ST section and T ripples. Then put to death rat, get each group of rat cardiac ventricular flesh, homogenate in frozen water, the centrifugal 5min of l000r/min, gets supernatant as sample. With TBA method thought-read flesh MDA content, by pyrogallol method survey SOD activity. Separately get each group of rat cardiac ventricular flesh and shred, the cooling lower pulverize of nitrogen, adds 1mL acetone immediately after weighing, and room temperature is placed 15min, and in 90 DEG C of water-baths, about 4min volatilizes acetone, adds 5mL2mmol/LMgSO4, cooling after boiling water boiling 15min, the centrifugal 10min of 1000r/min, gets supernatant as sample, by the content of biloluminescence method working sample ATP.
The demonstration of detecting ECG result, with Normal group comparison, model group Rat Ecg ST section is obviously raised, and T wave height point obviously, shows that Model of Acute Myocardial Ischemia copies successfully; Spun gold boisiana extract high dose group Rat Ecg ST section is slightly raised, and low dose group Rat Ecg ST section is obviously raised, and shows that high dose spun gold boisiana extract is improved effect to rat model ECG ST section.
The impact of spun gold boisiana extract active on rat model SOD of heart tissue in MDA content is in Table l. Result of the test by table 1 can find out, with Normal group comparison, model control group rat heart muscle SOD activity significantly weakens, MDA content significantly raise (P< 0.01), show that rat heart muscle oxidation resistance weakens. With model control group comparison, spun gold boisiana extract high and low dose group rat heart muscle SOD is active significantly to be strengthened (P< 0.01), MDA content significantly reduce (P< 0.01 orP< 0.05). This result shows that spun gold boisiana extract has certain antioxidation, and is dose-effect relationship.
Table l respectively organizes rat heart muscle SOD activity and the comparison of MDA content
Model control group and Normal group comparison,● P＜0.05，●● P＜0.01；
Treatment group and model control group comparison,★ P＜0.05，★★ P＜0.01。
Spun gold boisiana extract on the impact of rat model myocardium ATP content in table 2. Result of the test by table 2 can find out, with Normal group comparison, model control group rat heart muscle ATP content significantly reduce (P< 0.01), show that rat heart muscle energy supply is abnormal. With model group comparison, spun gold boisiana extract high and low dose group rat heart muscle ATP content significantly raise (P< 0.01 orP< 0.05). This result shows that spun gold boisiana extract has the abnormal effect of the myocardium energy supply of certain improvement, and is dose-effect relationship.
The comparison of the each group of table 2 rat heart muscle ATP content
Model control group and Normal group comparison,● P＜0.05，●● P＜0.01；
Treatment group and model control group comparison,★ P＜0.05，★★ P＜0.01。
Claims (7)
1. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury, it is characterized in that: described spun gold boisiana extract is prepared from by the following method: get spun gold rattan raw material, add aqueous sodium carbonate refluxing extraction, extract regulates pH=2.8-3.2 with hydrochloric acid, leave standstill, filter, get precipitation and be dried, obtain spun gold boisiana extract;
The dry tender leaf of described spun gold rattan raw material choose Oleaceae plants leaf of cinnamomi Jasmine JasminumcinnamomifoliumKobuski.
2. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 1, is characterized in that: the concentration of described aqueous sodium carbonate is 3%-10%.
3. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 2, is characterized in that: the concentration of described aqueous sodium carbonate is 4%-7%.
4. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 3, is characterized in that: the 8-15 that the consumption of described aqueous sodium carbonate is raw material weight doubly.
5. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 1, is characterized in that: the number of times of described refluxing extraction is 1-4 time, boils 2-4h at every turn.
6. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 1, is characterized in that: described extract acid solution regulates pH=3.0.
7. the purposes of spun gold boisiana extract in the medicine of preparing anti-myocardial and be subject to Free radicals injury according to claim 1, is characterized in that: the time of described standing step is 4-24h.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410365140.9A CN104083444B (en) | 2014-07-29 | 2014-07-29 | A kind of medical composition and its use resisted myocardial ischemia |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410365140.9A Division CN104083444B (en) | 2014-07-29 | 2014-07-29 | A kind of medical composition and its use resisted myocardial ischemia |
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| CN201610075413.5A Withdrawn CN105596431A (en) | 2014-07-29 | 2014-07-29 | Application of jasminum cinnamomifolium kobuski extract in preparation of medicine improving electrocardiographic abnormality of patient suffering from acute myocardial ischemia |
| CN201410365140.9A Active CN104083444B (en) | 2014-07-29 | 2014-07-29 | A kind of medical composition and its use resisted myocardial ischemia |
| CN201610075451.0A Withdrawn CN105596432A (en) | 2014-07-29 | 2014-07-29 | Application of jasminum cinnamomifolium kobuski extract in preparation of medicine resisting free radical oxidation damage of cardiac muscle cells |
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| CN201610075413.5A Withdrawn CN105596431A (en) | 2014-07-29 | 2014-07-29 | Application of jasminum cinnamomifolium kobuski extract in preparation of medicine improving electrocardiographic abnormality of patient suffering from acute myocardial ischemia |
| CN201410365140.9A Active CN104083444B (en) | 2014-07-29 | 2014-07-29 | A kind of medical composition and its use resisted myocardial ischemia |
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| CN1813902A (en) * | 2005-12-02 | 2006-08-09 | 蓝子花 | Salacia medicinal liquor for dispelling wetness-evil and stopping pain |
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| CN104083444B (en) | 2016-04-13 |
| CN105596431A (en) | 2016-05-25 |
| CN104083444A (en) | 2014-10-08 |
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Inventor after: You Fuli Inventor after: Liu Xuejian Inventor before: Liu Xuejian |
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Application publication date: 20160525 |