KR101611966B1 - Production of antlers and medicinal herbs extract for arthritis care medicine - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for enhancing stamina and treating inflammation of joints using antler extract of Chinese medicine and MSM, which comprises extracting antler antioxidant and a predetermined herbal medicine, extracting it with hot water to obtain a herbal medicine extract, A method for preparing a pharmaceutical composition having a stamina enhancing effect and a joint inflammation therapeutic effect is disclosed.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for producing a pharmaceutical composition,

More particularly, the present invention relates to a pharmaceutical composition using antler extract containing an antler, and to a method for preparing the same, and more particularly, to a method for preparing a medicinal composition for antler by mixing antler antler with a predetermined medicinal herb and at least one mushroom, And a method for producing the same. The present invention also provides a novel antler-containing pharmaceutical composition for improving stamina and treating joint inflammation.

The prevalence of arthritis is estimated to be around 0.8% worldwide. There are more than 500,000 patients in Korea, and about 80% of people over 55 years of age have degenerative knee arthritis on radiology. Conservative treatments for degenerative arthritis include nonpharmacologic physical therapy and exercise therapy. Drug therapy (NSAIDs, etc.) and intra-articular injection are used when non-drug treatment is ineffective. However, NSAIDs, which are commonly used as therapeutic agents for degenerative arthritis, cause systemic side effects such as digestive disorders, gastrointestinal disorders, and decreased renal function. As the patient's age increases, the incidence of side effects increases. Overdosage has many problems. NSAIDs work by inhibiting the synthesis of prostaglandins by cyclooxygenase enzymes. Prostaglandins are destroyed in the lungs if they are made in small amounts in many tissues. It is a major side effect of the action on the filamentous roots of the uterus, blood vessels, organs, platelet aggregation, and decreased secretion of gastric acid. Causing an increase in flare and local blood flow, and may interfere with the vasoconstriction of norepinephrine and angiotensin. Despite these side effects, however, it is also difficult to expect a satisfactory therapeutic effect.

Korean Patent Publication No. 10-2001-0097872 Korean Patent Laid-Open No. 10-2005-0104875 Korean Patent No. 100729513

The present invention has been made to solve the above-mentioned conventional problems, and it is an object of the present invention to improve the stamina of osteoarthritis patients and improve the stamina of osteoarthritis patients by using a dry mixture obtained by hot-water extracting various herbal medicine and mushroom mixture including antler, A composition for treating inflammation is provided. In order to accomplish this, in the present invention, NIAIDs (Nabumetone), steroids (Dexamethasone), MSM, antler extracts containing herbs, mixtures (MSM + antler) using a CIA (collagen-induced arthritis) The efficacy and safety of antler extracts were compared by comparing the treadmill exercise duration, arthritis index, and joint histological changes of each group. In addition, (VAS, Visual Analog Scale), WOMAC (Short-Form-12) Health Survey Score, and SF-12 ) In order to demonstrate the efficacy of the pharmaceutical composition according to the present invention.

As a means for attaining the object of the present invention, there is provided a method for preparing a medicinal herb including antler, comprising the steps of: 0.001-40% by weight of antler, 2-10% by weight of horseradish, Weighed so that the weight of the pearl bean is from 10 to 50 wt%, from about 2 to about 20 wt%, from about 2 to about 30 wt%, from about 1 to about 10 wt% of licorice, from about 1 to about 20 wt% The mixture was mixed with 2 to 50% by weight of at least one mushroom selected from the group consisting of Mushroom mushroom, Mushroom mushroom, Cordyceps mushroom, Ganoderma mushroom, Mushroom mushroom, Mushroom mushroom and Shiitake mushroom, An extracting step of adding water corresponding to the drainage weight to a hot water tank and heating the hot water extract to a temperature of 100 ° C or more for 6 hours to 24 hours and a step of cooling the mixed extract of herbal medicine and mushroom containing deer antioxidant extracted from the hot water tank to room temperature, The extract solution Which is obtained by filtration of the extract to obtain a filtrate or by using a conventional method of freeze-drying or hot-air spray drying the extract to prepare an extract of a perennial antioxidant, By mixing 10 to 80% by weight of MSM in the extracted mixed powder, the present invention has been completed by completing a composition for strengthening stamina as well as improving basic physical strength and for reducing and treating degenerative joint inflammation.

The pharmaceutical composition according to the present invention is primarily capable of controlling the biological rhythm by the various physiologically active ingredients of antler, various herbal medicine, and mushroom, and improving the stamina through the same, The blood circulation can be improved and the mental health index can be improved by this, so that the activity can be increased and the basic physical strength can be increased, so that each function of the body can be improved. The mixture of antler antler extract and MSM, which cause the improvement of the body function, was able to further enhance the effect of alleviating joint inflammation and provided a way to utilize it as a composition for treating joint inflammation.

1 is a photograph showing a joint inflammation relieving test by an experimental animal,
2 is a photograph showing the measurement of the distance of exercise by the treadmill of an experimental animal,
Figure 3 shows an identification diagram showing a visual similarity measure,
FIG. 4 is a photograph showing tissue regeneration ability by MSM alone; and
FIG. 5 is a photograph showing tissue regeneration test according to administration of a composition containing MSM.

Hereinafter, the present invention will be described in detail with reference to examples.

Deer antler is an untrimmed young horn of the deer. It is one of the herbal medicines used as an excellent antiseptic and tonic for ginseng. It has been widely used for the purpose of treating diseases through folk remedies. The antler includes hexose, pentose, hexoamnine, uronic acid, free amino acids, minerals, prostaglandins such as a kind of special fatty acids, gangliosides in glycolipids, hyaluronic acid hyaluronic acid) and many other biological hormones. It has been reported that antler antioxidant, anti-fatigue, anti-stress, antioxidant, antithrombotic, anti-aging, blood pressure lowering and anti-inflammatory effect, and according to domestic studies, growth promotion, cholesterol lowering, hepatocyte and liver function , Hematopoiesis, and thyroid function recovery. However, it is still insufficient to be recognized for the efficacy of antler in numerical and qualitative aspects.

Hwang Jung is a perennial plant of pizza plant known as Rongoli. It is an anti-pathogenic microorganism that suppresses the development of typhoid, yellow primary bacillus, improvement bacterium, tubercle bacillus, etc .; protection of cardiovascular system which increases blood flow of cardiac coronary artery, , beta-lipoprotein, and hyperlipidemic action that lower the cholesterol level.

Hwanggi is a perennial herb that belongs to the legume which mainly uses roots. It improves the heart function and strengthens the nourishment as mitigating tonic agent. It has diuretic, laxative, sugar, and exciting action. , Which is known to weaken the circulation and enhance the absorption of lesion materials, thereby promoting the augmenting ability to the new tissue.

Angelica is a perennial herb that has been known to be effective against various female diseases as a herb for women. Its root is derived from a derivative of Coumarin such as Decursin, Decursinol, Nodakenin, And a large amount of essential oils such as α-pinene, Limonene, β-oudesmol, and Elemol, which are used to regulate uterine function, sedation, analgesic action, , Diarrhea, and vitamin E deficiency.

It is the root of the perennial grasshopper (Ligusticum officinale Kitag) or Ligusticum jeholense Nakai belonging to the periwinkle, which is spicy and tastes warm, has good blood circulation, smoothes the menstrual flow, It is known that there is a pharmacological effect of eliminating pain (wind) and stopping pain.

Gugija is a herb that is distributed in Korea, China, and Japan. It is known to inhibit absorption of cholesterol into the intestines, prevent hypertension, and promote blood circulation.

The roots of rosemary are the medicinal herbs that are widely used for the treatment of colds, headaches and night blindness. The main functional ingredients contain atactilon, atlactilol and vitamins A and D, which are the main components of essential oils.

Sukjyanghwang is a medicinal herb that is processed with steamed roots of ginseng, plant rhubarb or rhizospheric sulfur and steamed and dried in the sun. Its properties are warm, tasty, slightly worn and poisonous. It protects the blood scarcely, It is known that it replenishes, fattenes and strengthens tendons and bones.

Mushrooms are rich in beta-glucan, which is a kind of fiber, guanylic acid, which reduces cholesterol in the blood, and minoric acid, which is an unsaturated fatty acid that prevents thrombosis, and can be expected to normalize blood glucose, blood pressure and blood cholesterol. In addition, since ancient times, mushrooms have been used as important food for preventing adult diseases and are recognized as foods effective for diabetes, hyperlipemia, arteriosclerosis, hypertension, digestive ulcer, bronchitis and kidney failure. It can be obtained at the same time.

MSM (methylsulfonylmethane) is a natural product produced by the oxidation process of dimethyl sulfoxide (DMSO). It is formulated into food additives, medicines and cosmetics. It is also used as an anticancer agent, antidote, muscle blocker, pain reliever, antidepressant, , Skin external agent, and the like. The actions of MSM known so far include pain relief, inflammation inhibition, application as a drug delivery carrier in an excellent drug delivery system, improvement of blood vessel swelling and blood flow, improvement of constipation by aging and intestinal interactions, collagen ) To promote the regeneration of scar tissue. It is intended to provide a way to maximize the effect of alleviating joint inflammation by mixing it with the efficacy of Korean medicine such as antler.

In the present invention, the functional ingredient is hydrolyzed in a variety of herbal medicines whose functions have been confirmed as described above, and the resulting mixture is used as a functional mixture for enhancing stamina and improving body function. MSM is added to the mixture to form a composition for alleviating and treating degenerative arthritis And the embodiments described below do not limit the scope of the present invention.

Preparation of herbal medicinal herbs containing antler

The Korean native antler was used as the antler product. The Korean medicine was washed with running water and shaded to remove moisture. In order to prepare antler extracts, 50 g of antler, 10 g of Hwangjeong, 70 g of Angelica gigas, 40 g of Angelica gigas, 35 g of Ganoderma lucidum, 20 g of Gugija, 12 g of ginseng, 6 g of licorice, 9 g of cinnamon, Is added to a hot water bath, and 1.36 liters of water, which is about five times the total solids, is added and heated at 100 DEG C or more for 12 hours to obtain a hot water extract, or the extract is lyophilized or spray dried and pulverized. In the present invention, an extract powder was prepared by spray drying, and the efficiency of spray drying was increased by using 50% of dextrin as an excipient in spray drying. MSM used raw materials sold by US Cardinal.

Animal experiment and drug administration

Animals were divided into 7 groups of 7-week-old male SD rats (control group, control group, Nabumetone, Dexamethasone, antler combination medicinal herb extract, MSM, antler mixture medicinal herb extract and MSM mixture) 8 dogs) were used in total 51 dogs. 1) were housed separately (Fig. 1), reared at an aseptic facility controlled at a temperature of 20-25 ° C and a humidity of 30-35%, and the light-dark cycle was adjusted to 12:12 hours. Feeds and water were supplied and adapted to the laboratory environment for one week before use in the experiment. After 1 week of adaptation period, all SD rats except non-treated group were injected with 0.3 ml of Zoletil 50 (purchased from PowerLab) diluted 10-fold and intramuscularly injected. (Complete Freund's adjuvant, CFA, Chondrex, USA) was injected (0.15 ml, 250 ug) to induce arthritis. Rats treated under anesthesia recovered from anesthesia within 30 minutes without any problems. The drug dose of each group was calculated as the dose of Nabumetone 79 mg / kg, Dexamethasone 0.5 mg / kg, antler extract 1493 mg / kg, MSM 25 mg / kg, / Day.

Athletic performance evaluation

The maximum exercise distance was measured through the exhaustion exercise on the treadmill, and the electric stimulation was applied when slipping out on the treadmill. The treadmill was run at 10 ° and 30m / s tilt and recorded the total exercise time when the electric stimulation and other stimuli showed no reaction for 5 seconds and calculated the maximum exercise distance.

Arthritis evaluation and joint biopsy

After 2 weeks of arthritis-induced injection, measurements were taken at 4-week intervals from 1 week. Three observers assessed the severity by visual examination according to the degree of arthritis. In the absence of one point edema or swelling, severe edema and flare limited to 2-point foot or ankle joint, moderate edema and flaring from 3-point ankle joint to metatarsal, It is the case that severe edema and redness occur throughout. After 4 weeks, 4, 8, 12, and 16 weeks after the start of the experiment, histological examinations were performed. In the 4th week, no manipulation and control were performed. In the remaining weeks, 1 mouse was sacrificed for each group. Hematoxylin-eosin staining was performed after demineralization of the left knee joint arthritis-induced knee joint. Subsequently, it was commissioned to the ASAN PMC pathology center and examined by microscopy (CARL ZEISS, x 40) for intimal hyperplasia ), Lymphocyte / plasmocytic infiltration, subintimal fibrosis, and vascularity were evaluated, and the total points were compared with a total of 12 points (0-3 points per item). Statistical analysis was performed using the SPSS 12.0 window program. Changes in measured values were analyzed using Oneway ANOVA. P values less than 0.05 were considered statistically significant.

Clinical trial design

On the anteroposterior and lateral radiographs of bilateral knee joints taken at the time of preloading, there was no significant difference between the two groups. Kellgren-Lawrence grade III, grade I suspicious or small bony spur, grade II clear spondylolisthesis, Grade IV osteosynthesis, severely narrowed joint space), and patients with bilateral knee pain who were not previously treated with degenerative knee osteoarthritis. Ten patients were treated with Nabumetone, Dexamethasone, Mixed medicinal herb extracts, MSM group, and antler mix medicinal herb extract / MSM mixed group, and 10 control groups were also selected. To ensure the homogeneity of the study subjects, the study was limited to men aged 60 to 80 years. The study period was set from January 9, 2012 to January 13, 2012, taking medication from January 16, 2012 to 2012 April 15, April 20, 2012 to April 20, 2012 after the post evaluation.

Taking clinical trial medication

The drug dose of each group was determined by oral administration of Nabumetone 79 mg / kg, Dexamethasone 0.5 mg / kg, 1493 mg / kg of antler mixed herbal extract and MSM 25 mg / kg once / . Statistical analysis was performed using the SPSS 12.0 window program. The change in measured values before and after treatment was analyzed. The paired t-test was used for the measurements. A p-value less than 0.05 was considered statistically significant.

Clinical Test Test Method

Pain measurements were measured using the Visual Analog Scale (VAS, Figure 3). The visual analog scale was a method of indicating the degree of pain above a certain straight line. The degree of pain was evaluated by indicating the intensity of the pain felt by the patient himself, with 0cm and 10cm in the absence of pain on a straight line of 10cm.

The Western Ontario and McMaster Universities Index (WOMAC) is a tool developed by Bellamy (1989) to measure the functional status of osteoarthritis patients. The measurement was from 0 to 96 points.

The SF-12 (Short Form-12) health recovery score was a simple abbreviation of SF-36. It consisted of 9 sub-domains and 12 items. The physical component score (PCS), mental health index component score, MCS), and the calculation method using each factor was calculated by Qualitymetric Inc., a copyright holder of SF-12.

Of antler extract Stamina  Improvement effect

The stamina enhancement effect by each treatment was measured by the increase of the exercise distance using the treadmill. The mean treadmill maximum exercise distance of each group was 78.31 m in the control group, 125.78 m in the Dexamethasone group, 138.71 m in the Nabumetone group, 164.71 m in the Nabumetone group, 164.71 m in the Nexumetone group, 142.57 meters, and the mixed group was 224.87 meters (Table 1). (P = 0.053), Nabumetone group (p = 0.365), MSM group (p = 0.541), and the other groups were significantly higher than those of the mixed group ) Were not statistically significant. The MSM group had a mean treadmill maximum exercise distance of 142.57 meters, higher than the Dexamethasone and Nabumetone groups, but the Dexamethasone group (p = 0.790). There was no statistically significant difference from the Nabumetone group (p = 1.000) and the antler mixed herbal medicine extract group (p = 0.541). It is believed that the other test group is a highly effective therapeutic or functional ingredient for alleviating joint inflammation. Although the antler mix herbal medicine extract somewhat helps alleviate joint inflammation, it strengthens stamina in patients suffering from joint inflammation, This is because it improves body rhythm and greatly improves mobility. In the mixed group, the mean treadmill maximum exercise distance was 224.9 meters. Statistical analysis showed that the superiority of the control group and the other groups showed superior exercise ability, and the herbal medicine extract, including antler, greatly contributes to the improvement of stamina (Table 2).

Control group Dexamethasone group Nabumetone group Antler extract group MSM group Mixed group Average Treadmill Maximum Exercise Distance 78.31 + - 23.43 125.78 ± 27.52 138.71 + - 10.00 164.71 + - 38.15 142.57 ± 10.38 224.87 ± 24.42

<Table 1> Average Treadmill Maximum Exercise Distance by Each Group>

Control group Dexamethasone group Nabumetone group Antler extract group MSM group Mixed group Control group .010 .001 .000 .000 .000 Dexamethasone group .010 .918 .053 .790 .000 Nabumetone group .001 .918 .365 1,000 .000 Antler .000 .053 .365 .541 .000 MSM group .000 .790 1,000 .541 .001 Mixed group .000 .000 .000 .001 .000

<Table 2> Statistical significance (p-value) of maximum exercise distance of treadmill by each group>

Improvement of arthritis in mice

Arthritis of the experimental rats in each group was evaluated at intervals of 4 weeks across the tea. The mean scores of arthritis were 2.85 points in the control group, 1.59 points in the Dexamethasone group, 1.55 points in the Nabumetone group, 2.28 points in the antler mixed herbal extract group, 1.91 points in the MSM group, and 1.65 points in the mixed group (Table 3). Statistically significant reductions in arthritis index (p = 0.000) were observed in the Dexamethasone and Nabumetone groups compared to the control group. There was a statistically significant decrease in arthritis index (p = 0.000) in MSM group and no statistically significant difference in arthritis index between Nabumetone group (p = 0.069) and Dexamethasone group (p = 0.154) , Indicating that MSM group is effective in decreasing arthritis index with Nabumetone group and Dexamethasone group. (P = 0.000) and there was no statistically significant difference (p = 0.023) with the Nabumetone group in the mixed group compared with the control group. However, there was no statistically significant difference (p = 0.038) between the herbal medicinal herb extract group and the control group, but there was a statistical difference between the medicinal herb extract group and the other medicines. (Table 4). When this result is compared with Example 8, it can be seen that, when the herbal mixed medicinal herb extracts contribute to alleviation of joint inflammation, it is effective for improvement of body rhythm such as improvement of stamina, The effect is increased. Therefore, in order to achieve the purpose of the treatment of joint inflammation through the improvement of body rhythm and the recovery of health which the present invention intends to achieve, it can be considered that the extract of antler containing herbal medicine greatly contributes.

Control group Dexamethasone group Dexamethasone group Antler extract group MSM group Mixed group Average
Arthritic index 2.85 ± 0.08 1.59 ± 0.31 1.55 + 0.09 2.28 0.38 1.91 + - 0.10 1.65 ± 0.23

<Table 3> Average arthritis index by each group>

Control group Dexamethasone group Nabumetone group Antler extract group MSM group Mixed group Control group .000 .000 .038 .000 .000 Dexamethasone group .000 .999 .000 .154 .058 Nabumetone group .000 .999 .000 .069 .023 Antler .058 .000 .000 .001 .003 MSM group .000 .154 .069 .001 .997 Mixed group .000 .058 .023 .003 .997

Table 4. Statistical significance of arthritis index (p-value)

Joint biopsy

One group per each group was sacrificed at 4-week intervals (sacrifice only in the first group and no control group) at 4-week intervals. As a result, the antler-mixed medicinal herb extract group showed 9 Point and tertiary biopsy showed 7 points lower than the control group. There were 7 points in the secondary histology, 7 points in the secondary histology, 7 points in the tertiary histology and 4 points in the Dexamethasone group and 4 points in the secondary histology. (Table 4). The mean scores of the Nabumetone group were similar to those of the Nabumetone group. When the herbal medicine extract of MSM was mixed with herbal medicine, the degree of inflammation of the hyaluronic acid was lowered as well as the arthritis index (Example 9). Thus, It is believed that it plays a role of helping the treatment of joint inflammation by improving the function of the body such as stamina, that is, the role of helping the efficacy of M. SM rather than the cycle.

Control group Dexamethasone group Nabumetone group Antler extract group MSM group Mixed group Primary Secondary Third Primary Secondary Third Primary Secondary Third Primary Secondary Third Primary Secondary Third Primary Secondary Third Zenon Castle
Initial cell number 0 One 0 0 0 0 0 One 0 0 One 0 One Lymphocyte
Circular cant 3 3 3 3 2 3 0 2 2 3 3 2 0 Close
fibrosis 3 3 3 2 One 2 2 3 2 2 One 3 2 Hemorrhage 3 3 3 2 One 2 3 3 3 3 2 2 3 Total score 9 10 9 7 4 7 5 9 7 8 7 7 6

<Table 5> Histopathological examination of each group>

Clinical effect

In clinical trials of the effects of each treatment group on pain relief in patients with joint inflammation, the visual superiority scale decreased from an average of 6.3 before administration to 3.1 after administration of nabumetone (p = 0.0002) Dexamethasone group decreased from 6.5 to 4.4 on average (p = 0.0012). MSM group decreased from 6.7 points before the administration to 3.6 points after the administration (p = 0.0004). In the mixed group, the addition of the antler combination medicinal extract showed a larger decrease than the MSM Decrease, p = 0.0005). On the other hand, the concentration of antler in the herbal medicinal herb extract group was 5.9 (p = 0.192) before 6.8 (p = 0.192) and 6.4 (p = 0.201) . There was a statistically significant decrease in pain in all groups except the control and antler herbal medicine extract groups (Table 6).

Control group Nabumetone group Dexamethasone group Antler extract group MSM group Mixed group Before dosing 6.9 ± 0.25 6.3 ± 0.21 6.5 ± 0.23 6.8 ± 0.31 6.7 ± 0.11 6.1 ± 0.19 After dosing 6.4 ± 0.27 3.1 ± 0.12 4.4 ± 0.18 5.9 ± 0.18 3.6 ± 0.15 3.2 ± 0.25 p-value 0.201 0.002 0.012 0.192 0.004 0.005

<Table 6> Changes in visual analog scale before and after drug administration in each group>

In the WOMAC group, the Nabumetone group decreased from 38.5 before administration to 26.3 after administration (p = 0.001). In the Dexamethasone group, it decreased from 37.1 before administration to 25.9 after administration (p = 0.0001) And decreased from 37.4 before the administration to 29.6 after the administration (p = 0.035). The mixed group also decreased from 38.1 before the administration to 29.1 after the administration (p = 0.021). The antimicrobial herbal extract powder group decreased from 37.9 before administration to 30.2 after administration (p = 0.042), and the WOMAC scale showed a statistically significant decrease in all but the control group (Table 7). Therefore, as shown in 'Example 8-10', herbal medicine extract containing antler is very effective in improving the health condition of the patient, and the health of the patient thus improved is very positive for increasing the effect of the joint inflammation treatment .

Control group Nabumetone group Dexamethasone group Antler extract group MSM group Mixed group Before dosing 37.7 ± 0.25 38.5 ± 0.21 37.1 ± 0.23 37.9 ± 0.31 37.4 ± 0.11 38.1 ± 0.19 After dosing 35.6 ± 0.27 26.3 ± 0.12 25.9 ± 0.18 30.2 ± 0.18 29.6 ± 0.15 29.1 ± 0.25 p-value 0.452 0.001 0.001 0.042 0.035 0.021

<Table 7> Changes of WOMAC Scale Before and After Administration of Each Drug Group>

The SF-12 index was significantly higher in the Dexamethasone group compared to the SF-12 (Short Form-12). The SF-12 index increased to 47.9 from the mean of 42.5 in the physical fitness index (MCS) Increased from 43.1 on average to 44.5 on average. In the Nabumetone group, the physical health index decreased from 43.5 to 41.8 and the mental health index increased from 43.8 to 46.7. The physical health index increased significantly from 40.1 to 48.9 in the antler extract group, and the physical health index increased from 42.9 to 49.8 in the mixed group. As a result, it was found that the physical health index was significantly increased in the group taking the antler as compared with the other groups. As a result, it was found that the herb medicine such as antler was very effective in improving the physical function. This is because the antler mixture extract prepared in the present invention is very effective in enhancing the effect of the composition for alleviating or treating joint inflammation and is added to the composition for the treatment of joint inflammation so as to be a very important factor for completing the invention of the therapeutic composition (Table 8).

Control group Dexamethasone group Nabumetone group Antler extract group MSM group Mixed group PCS MCS PCS MCS PCS MCS PCS MCS PCS MCS PCS MCS Before dosing 42.1 ±
7.56 44.1 ±
8.62 42.5 ±
9.42 43.1 ±
6.84 43.5 ±
7.41 43.8 ±
5.84 40.1 ±
7.23 41.5 ±
7.56 42.9 ±
5.81 43.5 ±
6.81 42.9 ±
6.56 41.2 ±
7.12 After dosing 42.3 ±
6.21 44.5 ±
6.52 47.9 ±
7.43 44.5 ±
6.35 41.8 ±
8.47 46.7 ±
6.11 48.9 ±
6.13 49.2 ±
5.96 44.7 ±
6.86 42.7 ±
5.23 49.8 ±
6.78 46.5 ±
6.56 p-value 0.946 0.917 0.546 0.897 0.741 0.602 0.231 0.147 0.782 0.891 0.289 0.451

<Table 8> Changes of SF-12 before and after administration of each drug>

Claims (6)

A method for producing a pharmaceutical composition using antler containing herbal medicine and MSM, comprising the steps of: 0.001-40% by weight of antler, 2-10% by weight of hibernation, 10-50% by weight of sulfur, 10-50% by weight of Angelica gigas, 2 ~ 20% by weight of guaji, 2 ~ 30% by weight of liquorice, 1~10% by weight of licorice, 1~20% by weight of cinnamon, 2~50% by weight of sorghum persimmon, mushroom, mushroom, 2 to 50% by weight of at least one mushroom selected from the group consisting of mushroom, shiitake mushroom, and shiitake mushroom is mixed, and 2 to 8 times the weight of the mushroom is heated to 100 ° C or higher for 6 to 24 hours, (10) to 80% by weight of methylsulfonylmethane ( MSM ) is added to the extract of the antler containing Chinese medicine obtained by cooling the hot water bath to room temperature and filtering the hot water extract through a microfilter to obtain a filtrate. The method of learning the composition.
delete delete A pharmaceutical composition for alleviating inflammation of joints comprising an extract of antler-containing herbal medicine prepared according to claim 1 or 2 and an EMM mixture as an active ingredient.
A health supplement composition for health promotion comprising an extract of antler herb medicine prepared according to any one of claims 1 or 2 and an EMM mixture as an active ingredient.
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