CN105588821A - Total-reflection-type laser induced fluorescence confocal scanning device and method - Google Patents
Total-reflection-type laser induced fluorescence confocal scanning device and method Download PDFInfo
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- CN105588821A CN105588821A CN201410559361.XA CN201410559361A CN105588821A CN 105588821 A CN105588821 A CN 105588821A CN 201410559361 A CN201410559361 A CN 201410559361A CN 105588821 A CN105588821 A CN 105588821A
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Abstract
The invention provides a total-reflection-type laser induced fluorescence confocal scanning device and method. The device comprises a laser device, a macroporous diaphragm, a collimating mirror, a beam splitter mirror, an object lens, a to-be-measured chip, an object support, a convergent mirror, an aperture diaphragm, a photoelectric detector, and a computer. The laser emitted by the laser device passes through the macroporous diaphragm, the collimating mirror, the beam splitter mirror and the object lens and then is irradiated to the to-be-measured chip, the to-be-measured chip is excited to emit another type of fluorescence, the other fluorescence passes through the object lens and the beam splitter mirror and then is reflected to the convergent mirror, the other convergent fluorescence passes through the aperture diaphragm and is received by the photoelectric detector, and then is transmitted to the computer to obtain the scan information. The device and the method have the beneficial effect that excitation of the to-be-measured chip by a spot light source is guaranteed, point imaging reception is carried out, mutual interference due to luminescence at different positions can be avoided, collection efficiency and scan sensitivity of fluorescence can be increased, operation is easy and convenient, and the device and the method can be widely used in the field of biology chip detection.
Description
Technical field
The invention belongs to biological chip testing technology field, especially relate to a kind of total reflection type laser and lureLead fluorescence co-focusing scan device and method.
Background technology
Biochip is just being widely used in life science, medical research as the emerging high-tech product of oneAnd application. In existing biological chip testing technology, main technological means has confocal scanning methodWith the detection method based on CCD (Charge-coupleddevice), because confocal scanning method hasHigher sensitivity, is convenient to the analysis of testing result, and most micro-array biochip scanner allAdopt confocal scanning principle. But in common laser-induction fluorescence co-focusing scan device, glimmeringLight signal collection light path is coaxial with fluorescent emission point, makes the fluorescence of induced with laser generation to space everywhereTransmitting, only has the fluorescence signal of small scale very collected, causes fluorescent collecting efficiency not high; Simultaneously byWhat adopt in fluorescence excitation is xenon lamp, and the laser beam intensity of generation is low, of poor quality, makes fluorescenceIt is less that matter is excited to the molecular number of excitation state, the fluorescence intensity that fluorescent material produces also a little less than, thereforeExisting checkout gear sensitivity is also lower.
Summary of the invention
The object of this invention is to provide a kind of scan sensitivity high, the total-reflection type that fluorescent collecting efficiency is highLaser-induction fluorescence co-focusing scan device and method, the scanning that is especially applicable to biochip detects.
For reaching above-mentioned purpose, the technical solution used in the present invention is: a kind of total reflection type laser induced glimmeringLight co-focusing scan device, comprises laser instrument, macropore diaphragm, collimating mirror, beam splitter, object lens, treatsSurvey chip, article carrying platform, convergent mirror, aperture, photodetector, computer, its feature existsIn: under described laser instrument, be placed with described macropore diaphragm, under described macropore diaphragm, be placed withDescribed collimating mirror, is placed with described beam splitter under described collimating mirror, described beam splitter and ground are45 ° of placements, are placed with described object lens under described beam splitter, the mirror heart of described object lens, described standardDescribed in the mirror heart of straight mirror, the Kong Xinyu of described macropore diaphragm, the light outlets of laser instrument is located along the same line,Described chip to be measured is put on adjustable described article carrying platform, and described beam splitter front-right is vertically placed withDescribed convergent mirror, the front-right of described convergent mirror is placed with described aperture, described apertureThe hole heart is positioned on the vertical line of the described convergent mirror mirror heart, and described aperture front-right is placed with described photoelectricityDetector, described computer is connected with described photodetector.
Described laser instrument is green laser, and wavelength is 532nm.
Described macropore diaphragm is positioned at described collimating mirror front focus, and described aperture is positioned at described convergent mirrorBack focus.
Described chip to be measured is put on adjustable described article carrying platform, and described article carrying platform is by described levelFixed head, be affixed on described horizontal fixed head described in move horizontally plate, move horizontally plate described in being affixed onDescribed cross sliding clock composition.
Advantage and good effect that the present invention has are: owing to adopting technique scheme, laser intensity is carriedHeight, described chip to be measured can move freely, and has ensured that spot light excites described chip to be measured to carry out a littleImaging receives, and has avoided phase mutual interference, has improved the sensitivity of scanning; Described macropore diaphragm position simultaneouslyIn described collimating mirror front focus, described aperture is positioned at described convergent mirror back focus, and conjugation focuses onMode has effectively been removed veiling glare around, has improved the collection efficiency of fluorescence; Apparatus structure is simple, behaviourFacilitate, there is higher production practical value.
Brief description of the drawings
Fig. 1 is showing of a kind of total reflection type laser induced fluorescent common focusing scanning means of the present invention and methodIntention;
Fig. 2 is the biochip scanning schematic diagram in example of the present invention.
In figure:
1, laser instrument 2, macropore diaphragm 3, collimating mirror
4, beam splitter 5, object lens 6, chip to be measured
7, article carrying platform 8, convergent mirror 9, aperture
10, photodetector 11, computer 7-1, cross sliding clock
7-2, move horizontally plate 7-3, horizontal fixed head
Detailed description of the invention
Describe the specific embodiment of the present invention in detail below in conjunction with accompanying drawing.
As shown in Figure 1, the present invention include laser instrument 1, macropore diaphragm 2, collimating mirror 3, beam splitter 4,Object lens 5, chip to be measured 6, article carrying platform 7, convergent mirror 8, aperture 9, photodetector 10,Computer 11. Under laser instrument 1, be placed with macropore diaphragm 2, under macropore diaphragm 2, be placed withCollimating mirror 3, the position of collimating mirror 3 makes macropore diaphragm 2 be positioned at collimating mirror 3 front focus, collimating mirror 3Under be placed with beam splitter 4, beam splitter 4 is 45 ° of placements with ground, under beam splitter 4, putsBe equipped with object lens 5, the Kong Xinyu laser instrument of the mirror heart of object lens 5, the mirror heart of collimating mirror 3, macropore diaphragm 21 light outlets is located along the same line, and chip 6 to be measured is put on adjustable article carrying platform 7, and loading is flatPlatform 7 by horizontal fixed head 7-3, be affixed on moving horizontally plate 7-2, being affixed on level of horizontal fixed head 7-3The cross sliding clock 7-1 composition of movable plate 7-2, the front-right of beam splitter 4 is vertically placed with convergent mirror 8,The front-right of convergent mirror 8 is placed with aperture 9, and the hole heart of aperture 9 is positioned at rear Jiao of convergent mirror 8Point, aperture 9 front-rights are placed with photodetector 10, computer 11 and photodetector 10Be connected, obtain the information of chip 6 to be measured with this.
The course of work of this example:
(1) chip 6 to be measured is placed in to cross sliding clock 7-1 above, opens laser instrument 1 preheating, observe lightElectric explorer 10, sees and whether can receive optical signal, if can directly enter step (3),Can not enter step (2);
(2) mobile and horizontal slide block 7-1 regulates object lens 5 height simultaneously, and photodetector 10 can be connectReceive optical signal;
(3) expansion that the laser that laser instrument 1 sends forms through macropore diaphragm 2, collimating mirror 3, beam splitter 4Beam system expands, and then sees through object lens 5 and converges on chip 6 to be measured;
(4), under the exciting of laser, the fluorescence molecule on chip 6 to be measured absorbs light-wave energy, spontaneousGround releases energy fast, sends the fluorescence of another kind of wavelength, and the fluorescence of another kind of wavelength is logicalCross object lens 5, beam splitter 4 reflexes on convergent mirror 8;
(5) the another kind of fluorescence that convergent mirror 8 converges to is connect by photoelectric detector 10 through aperture 9Receive, the optical signal of this point is converted into the signal of telecommunication, input in computer 11;
(6) continue mobile and horizontal slide block 7-1, repeat above-mentioned steps, make laser point by point scanning whole to be measuredChip 6.
In sum, a kind of total reflection type laser induced fluorescent common focusing scanning means of the present invention, ensuresSpot light excite chip to be measured to carry out a some imaging to receive, avoided the luminous caused phase of diverse locationMutual interference, has improved the collection efficiency of fluorescence and the sensitivity of scanning, simple to operation, extensively shouldFor biochip test field.
Above one embodiment of the present of invention are had been described in detail, but described content is only of the present inventionPreferred embodiment, can not be considered to for limiting practical range of the present invention. All according to the present patent application modelEnclose done equalization variation and improvement etc., within all should still belonging to patent covering scope of the present invention.
Claims (4)
1. total reflection type laser induced fluorescent common focusing scanning means and a method, comprises laser instrument, largeHole diaphragm, collimating mirror, beam splitter, object lens, chip to be measured, article carrying platform, convergent mirror, aperture lightDoor screen, photodetector, computer, is characterized in that: under described laser instrument, be placed with described largeHole diaphragm, is placed with described collimating mirror under described macropore diaphragm, under described collimating mirror, placesHave described beam splitter, described beam splitter and ground are 45 ° of placements, under described beam splitter, are placed withDescribed object lens, the mirror heart of the mirror heart of described object lens, described collimating mirror, the Kong Xinyu of described macropore diaphragmThe light outlets of described laser instrument is located along the same line, and described chip to be measured is put in adjustable described loadingOn platform, the front-right of described beam splitter is vertically placed with described convergent mirror, the positive right side of described convergent mirrorSide is placed with described aperture, and the hole heart of described aperture is positioned at the vertical line of the described convergent mirror mirror heartUpper, described aperture front-right is placed with described photodetector, described computer and described photoelectricityDetector is connected.
A kind of total reflection type laser induced fluorescent common focusing scanning means according to claim 1 andMethod, is characterized in that: described laser instrument is green laser, and wavelength is 532nm.
A kind of total reflection type laser induced fluorescent common focusing scanning means according to claim 1 andMethod, is characterized in that: described macropore diaphragm is positioned at described collimating mirror front focus, described apertureBe positioned at described convergent mirror back focus.
A kind of total reflection type laser induced fluorescent common focusing scanning means according to claim 1 andMethod, is characterized in that: described chip to be measured is put on adjustable described article carrying platform, described loadingPlatform by described horizontal fixed head, be affixed on described horizontal fixed head described in move horizontally plate, be affixed on instituteState the described cross sliding clock composition that moves horizontally plate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410559361.XA CN105588821A (en) | 2014-10-18 | 2014-10-18 | Total-reflection-type laser induced fluorescence confocal scanning device and method |
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| CN201410559361.XA CN105588821A (en) | 2014-10-18 | 2014-10-18 | Total-reflection-type laser induced fluorescence confocal scanning device and method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106681015A (en) * | 2017-01-09 | 2017-05-17 | 成都曙光光纤网络有限责任公司 | Automatic focusing device and method suitable for reflection-type TeraHertz wave system |
| CN107335912A (en) * | 2017-06-05 | 2017-11-10 | 赵猛 | Coaxial profile-followed focusing system |
| CN109406478A (en) * | 2018-12-29 | 2019-03-01 | 上海市激光技术研究所 | Based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and method |
| CN109445081A (en) * | 2018-12-07 | 2019-03-08 | 哈尔滨工业大学 | A kind of high speed tracking scanning confocal micro-measurement apparatus and data processing method |
| CN114994892A (en) * | 2022-05-09 | 2022-09-02 | 中国科学院化学研究所 | Laser confocal microscopic imaging system and method |
-
2014
- 2014-10-18 CN CN201410559361.XA patent/CN105588821A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106681015A (en) * | 2017-01-09 | 2017-05-17 | 成都曙光光纤网络有限责任公司 | Automatic focusing device and method suitable for reflection-type TeraHertz wave system |
| CN107335912A (en) * | 2017-06-05 | 2017-11-10 | 赵猛 | Coaxial profile-followed focusing system |
| CN107335912B (en) * | 2017-06-05 | 2019-07-19 | 赵猛 | Coaxial profile-followed focusing system |
| CN109445081A (en) * | 2018-12-07 | 2019-03-08 | 哈尔滨工业大学 | A kind of high speed tracking scanning confocal micro-measurement apparatus and data processing method |
| CN109406478A (en) * | 2018-12-29 | 2019-03-01 | 上海市激光技术研究所 | Based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and method |
| CN109406478B (en) * | 2018-12-29 | 2024-05-31 | 上海市激光技术研究所有限公司 | Laser-induced fluorescence spectrum detection device based on liquid lens automatic focusing and use method thereof |
| CN114994892A (en) * | 2022-05-09 | 2022-09-02 | 中国科学院化学研究所 | Laser confocal microscopic imaging system and method |
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Application publication date: 20160518 |
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