CN105806816A - Detecting device and method for multichannel up-conversion luminescence - Google Patents
Detecting device and method for multichannel up-conversion luminescence Download PDFInfo
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- CN105806816A CN105806816A CN201610146631.3A CN201610146631A CN105806816A CN 105806816 A CN105806816 A CN 105806816A CN 201610146631 A CN201610146631 A CN 201610146631A CN 105806816 A CN105806816 A CN 105806816A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0461—Simultaneous, e.g. video imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
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Abstract
The invention discloses a detecting device and method for multichannel up-conversion luminescence.The detecting device comprises an exciting light path for exciting up-conversion luminescence samples to emit up-conversion fluorescence signals, a sample array pond for sampling the multiple up-conversion luminescence samples at the same time, a detecting module for collecting the up-conversion fluorescence signals of the up-conversion luminescence samples and converting the up-conversion fluorescence signals into fluorescence images and a mobile phone for receiving the fluorescence images and calculating to obtain the concentration information of the up-conversion luminescence samples.By means of the detecting method and device, the up-conversion luminescence signals of the multiple samples can be excited and collected at the same time, and the detecting method and device have the advantages of being high in sensitivity, good in linearity and linearity and the like.
Description
Technical field
The present invention relates to a kind of multichannel up-conversion luminescence detecting device and detection method, it is possible to be applied to the numerous areas such as biochemistry, aqueous foods, medical drugs.
Background technology
Up-conversion luminescent material is a kind of to excite the lower novel fluorescence marker material that can launch visible ray near infrared light, its the special characteristics of luminescence makes it play exclusive characteristic at detection field: first, owing to using near-infrared excitation light, major part material does not have fluorescence signal when this wave band excites, and drastically increases detection signal-to-noise ratio and sensitivity;Second, it can avoid sample etches and the fluorescent quenching caused that self decays;3rd, itself is all fanout free region for human body and environment.These characteristics of up-conversion luminescent material are that other fluorescent materials are incomparable, and it has a wide range of applications at biomedical sectors such as bio-imaging, fluorescent labeling, optical dynamic therapies.
In first technology, the device being specifically designed to up-conversion luminescent material detection is also fewer.Patent of invention CN201510253603.7 discloses a kind of method utilizing mobile phone detection up-conversion luminescence and device, and it is made up of excitation module, sample cell and detection module.Excitation module includes a quasiconductor exciter, and the exciting light that this laser instrument sends passes sequentially through prefilter and collimating mirror, and prefilter filters the light disturbance in infrared excitation light, and collimating mirror is used for focusing on infrared excitation light.Sample cell is the microscope slide with a shrinkage pool, or angled relationships perpendicular with excitation light path, it is possible to gather a up-conversion fluorescence sample.Detection module and excitation module be perpendicular or line relationship, it includes the smart mobile phone of rearmounted optical filter, two-sided concave mirror and built-in camera, rearmounted optical filter filters the infrared light interference in fluorescence, and two-sided concave mirror expands fluorescence hot spot, and smart mobile phone utilizes built-in camera obtain fluoroscopic image and analyze and process.During detection, a up-conversion fluorescence sample is collected by sample cell, a branch of exciting light that quasiconductor exciter sends, this exciting light after prefilter elimination veiling glare by collimating mirror focus on formed a high intensity excite hot spot, this excites hot spot to be irradiated in the upper switching sample in sample cell, excites sample to send visible fluorescence.The visible fluorescence being excited is amplified by concave mirror after rearmounted optical filter denoising and is imaged on smart mobile phone built-in camera, collected by camera is to fluorescence and is transferred to interior of mobile phone after being converted into image and carries out image procossing, the red, green, blue three primary colours component of fluorescence hot spot in mobile phone analysis of fluorescence image, by obtaining the concentration information of up-conversion fluorescence sample with standard curve contrast.
A lot of shortcoming is there is in above-mentioned detecting device in first technology in actual sample detects:
1, the focusing of exciting light is made excitation module cannot excite the up-conversion luminescence of multiple sample by collimating mirror simultaneously.Excitation module adopt the exciting light that semiconductor laser is sent by collimating mirror excite the upper transition detection sample on sample cell after focusing on, but the exciting light after focusing on has only formed a hot spot, thus a up-conversion fluorescence sample can only be excited, it is impossible to excite switching sample in two and two or more simultaneously.
2, the expansion of fluorescence hot spot is made detection module cannot obtain the fluorescence of multiple upper switching sample by two-sided concave mirror simultaneously.Detection module utilize the fluorescence hot spot that upper switching sample is sent by two-sided concave mirror expand and be imaged onto on mobile phone camera, fluorescence hot spot after expansion occupies mobile phone camera areas imaging, makes detection module cannot detect switching sample in two and two or more simultaneously.
Summary of the invention
It is an object of the invention to overcome the above-mentioned deficiency in first technology, it is provided that a kind of multichannel up-conversion luminescence detecting device and detection method, it is intended to can excite simultaneously and gather the up-conversion fluorescence signal of multiple sample.
This invention address that technical problem, following technical scheme of sampling
The multichannel up-conversion luminescence detecting device of the present invention, it is characterized in that: include for exciting up-conversion luminescence sample to send the excitation light path of up-conversion fluorescence signal, sample array pond for up-conversion luminescence sample of sampling, for gathering the up-conversion fluorescence signal of up-conversion luminescence sample and changing into the detection module of fluoroscopic image, and for receiving fluoroscopic image and calculating the mobile phone of the concentration information obtaining up-conversion luminescence sample;
Described excitation light path includes laser instrument, beam splitter and the conduction optical fiber being connected between described laser instrument and described beam splitter;Described laser instrument is used for sending a branch of near-infrared excitation light, described conduction optical fiber is input to beam splitter for the exciting light sent by laser instrument, described beam splitter, for a branch of exciting light received is divided into multi beam exciting light, excites the multiple up-conversion luminescence samples on sample array pond simultaneously;
Described sample array pond is positioned at the surface of beam splitter, for multiple up-conversion luminescence sample of sampling simultaneously;
Described detection module includes infrared fileter, wide-angle lens and luminous signal harvester;Described infrared fileter is for carrying out denoising to the multiple up-conversion fluorescence signals produced by multiple up-conversion luminescence samples on sample array pond, described wide-angle lens, for expanding the imaged viewing angle of luminous signal harvester, makes luminous signal harvester collect multiple up-conversion fluorescence signal simultaneously and change into fluoroscopic image;
Described mobile phone analyzes and processes after being used for receiving fluoroscopic image, calculates the concentration information obtaining multiple up-conversion fluorescence sample;
Described excitation light path, sample array pond and detection module are arranged in the shell of lucifuge, are provided with luminous signal harvester and the insert port in sample array pond on the housing.
Wherein: described beam splitter is one point of many types of fiber splitter.
Described sample array pond is the microscope slide with multiple shrinkage pools.
Described luminous signal harvester is the CMOS camera of mobile phone.
The method utilizing above-mentioned detecting device detection up-conversion luminescence, comprises the steps:
A, at least one up-conversion luminescence sample of sampling with sample array pond;
B, laser instrument send a branch of near-infrared excitation light, and the exciting light that laser instrument is sent by conduction optical fiber is input to beam splitter, and a branch of exciting light received is divided into multi beam exciting light by beam splitter, excites each the up-conversion luminescence sample on sample array pond simultaneously;
C, infrared fileter are imaged on the photosurface of luminous signal harvester by wide-angle lens, change into fluoroscopic image after the up-conversion fluorescence signal that each up-conversion luminescence sample produces is carried out denoising;
D, mobile phone analyze and process after receiving fluoroscopic image, calculate the concentration information obtaining each up-conversion fluorescence sample.
Compared with prior art, beneficial effects of the present invention is embodied in:
The multichannel up-conversion luminescence detecting device of the present invention, utilize sample array pond can gather multiple up-conversion luminescence sample simultaneously, utilize a branch of exciting light that laser instrument is exported by beam splitter after being divided into multi beam exciting light, to excite these multiple up-conversion luminescence samples simultaneously, wide-angle lens is utilized to expand luminous signal harvester imaged viewing angle so that luminous signal harvester can obtain the fluorescence signal of these multiple samples and be converted into fluoroscopic image simultaneously, mobile phone analysis of fluorescence image calculating is utilized to obtain the concentration information of multiple up-conversion fluorescence sample, therefore while inventive apparatus can be readily adapted multiple up-conversion fluorescence sample, synchronous detecting, detection efficiency is high, more practical, and have highly sensitive, the linearity is good and the feature such as good stability.
Accompanying drawing explanation
Fig. 1 is the structural representation of multichannel up-conversion luminescence detecting device of the present invention;
Fig. 2 is the example test result that the present invention detects four up-conversion luminescence samples simultaneously;
Number in the figure: 1 laser instrument;2 conduction optical fiber;3 shells;4 beam splitters;5 sample array ponds;6 infrared fileters;7 wide-angle lens;8 luminous signal harvesters;9 mobile phones.
Detailed description of the invention
For making the purpose of the present invention, technical scheme and effect become apparent from understanding, below with reference to the drawings and specific embodiments, the present invention is described in further details.It should be understood that following example are only in order to explain the present invention, and the present invention is not done any restriction.
Referring to Fig. 1, Fig. 1 is the structural representation of a kind of four-way up-conversion luminescence detecting device, it includes for exciting up-conversion luminescence sample to send the excitation light path of up-conversion fluorescence signal, sample array pond for up-conversion luminescence sample of sampling, for gathering the up-conversion fluorescence signal of up-conversion luminescence sample and changing into the detection module of fluoroscopic image, and for receiving fluoroscopic image and calculating the mobile phone of the concentration information obtaining up-conversion luminescence sample;
Wherein:
Excitation light path includes laser instrument 1, beam splitter 4 and the conduction optical fiber 2 being connected between laser instrument 1 and described beam splitter 4;Laser instrument 1 is used for sending a branch of near-infrared excitation light, conduction optical fiber 2 is for being input to beam splitter 4 by the exciting light that laser instrument 1 sends, beam splitter 4 is one point of four type fiber splitter, for a branch of exciting light received is divided into four bundle exciting lights, from four the up-conversion luminescence samples excited sample array pond 5 simultaneously;
Sample array pond 5 is the microscope slide with four shrinkage pools, is positioned at the surface of beam splitter 4, it is possible to four up-conversion luminescence samples of carrying simultaneously.
Detection module includes infrared fileter 6, wide-angle lens 7 and luminous signal harvester 8, four the up-conversion fluorescence signals produced on sample array pond 5 are by after infrared fileter 6 denoising, it is imaged on the photosurface of luminous signal harvester 8 by wide-angle lens 7, wide-angle lens 7 is the imaging lens that focal length is very short, expands luminous signal harvester 8 imaged viewing angle and makes luminous signal harvester 8 can collect four up-conversion fluorescence signals simultaneously and change into fluoroscopic image.Luminous signal harvester 8 is the CMOS camera that mobile phone 9 carries.
Mobile phone 9 analyzes and processes after being used for receiving fluoroscopic image, calculates the concentration information obtaining four up-conversion fluorescence samples;
Excitation light path, sample array pond and detection module are arranged in the metal shell 3 of lucifuge, are provided with luminous signal harvester 8 and the insert port in sample array pond 5 on shell 3.
Utilize above-mentioned detecting device that four different up-conversion luminescence samples carry out detection checking simultaneously:
Step 1, configure solution to be detected
First, it is respectively configured concentration and is all four kinds of up-conversion luminescence sample original solutions of 10mg/mL;Then, utilize deionized water to dilute four kinds of up-conversion luminescence sample original solutions respectively, obtain a series of detection solution that concentration is 5mg/mL, 2.5mg/mL, 1.25mg/mL, 0.625mg/mL and 0.3125mg/mL, encapsulate preservation respectively with brown delivery flask, stand-by.
Step 2: utilize detecting device in the present embodiment that the detection solution of four kinds of up-conversion luminescence samples is detected simultaneously, every kind of sample uses same passage, four different samples that each detectable concentration is identical, it is thus achieved that its fluorescence intensity.
Step 3: draw curve.
After the fluorescence intensity of four kinds of up-conversion luminescence samples of detection means measure of the present embodiment, draw the standard working curve of each passage respectively, as shown in Figure 2.
Fig. 2 illustrates, when the detecting device of the present embodiment detects four kinds of up-conversion luminescence samples simultaneously, along with the increase of sample solution concentration in each passage, the change curve of fluorescence intensity detected.
As from the foregoing, the working curve of each passage of the present embodiment detecting device has the good linearity, the present embodiment can gather simultaneously, excite and detect multiple up-conversion luminescence sample solution, can be widely applied to the fields such as biological and chemical metering, water quality and food analysis, medical science and drug inspection.
The foregoing is only the preferred embodiments of the present invention, it is not intended to limit the invention to or be constrained in above-mentioned implementation.All within the scope of the invention the made any amendment of the present invention and replacement be should be included in the scope of the claims in the present invention.
Claims (5)
1. a multichannel up-conversion luminescence detecting device, it is characterized in that: include for exciting up-conversion luminescence sample to send the excitation light path of up-conversion fluorescence signal, sample array pond for up-conversion luminescence sample of sampling, for gathering the up-conversion fluorescence signal of up-conversion luminescence sample and changing into the detection module of fluoroscopic image, and for receiving fluoroscopic image and calculating the mobile phone of the concentration information obtaining up-conversion luminescence sample;
Described excitation light path includes laser instrument (1), beam splitter (4) and the conduction optical fiber (2) being connected between described laser instrument (1) and described beam splitter (4);Described laser instrument (1) is used for sending a branch of near-infrared excitation light, described conduction optical fiber (2) is for being input to beam splitter (4) by the exciting light that laser instrument (1) sends, described beam splitter (4), for a branch of exciting light received is divided into multi beam exciting light, excites the multiple up-conversion luminescence samples on sample array pond (5) simultaneously;
Described sample array pond is positioned at the surface of beam splitter (4), for multiple up-conversion luminescence sample of sampling simultaneously;
Described detection module includes infrared fileter (6), wide-angle lens (7) and luminous signal harvester (8);Described infrared fileter (6) is for carrying out denoising to the multiple up-conversion fluorescence signals produced by multiple up-conversion luminescence samples on sample array pond (5), described wide-angle lens (7) is used for expanding the imaged viewing angle of luminous signal harvester (8), makes luminous signal harvester (8) collect multiple up-conversion fluorescence signal simultaneously and change into fluoroscopic image;
Described mobile phone (9) analyzes and processes after being used for receiving fluoroscopic image, calculates the concentration information obtaining multiple up-conversion fluorescence sample;
Described excitation light path, sample array pond and detection module are arranged in the shell (3) of lucifuge, are provided with luminous signal harvester (8) and the insert port of sample array pond (5) on described shell (3).
2. multichannel up-conversion luminescence detecting device according to claim 1, it is characterised in that: described beam splitter (4) is one point of many types of fiber splitter.
3. multichannel up-conversion luminescence detecting device according to claim 1, it is characterised in that: described sample array pond (5) is the microscope slide with multiple shrinkage pools.
4. multichannel up-conversion luminescence detecting device according to claim 1, it is characterised in that: described luminous signal harvester (8) is the CMOS camera that mobile phone (9) carries.
5. the method for the detecting device detection up-conversion luminescence that a kind utilizes described in claim 1, it is characterised in that comprise the steps:
A, at least one up-conversion luminescence sample of sampling with sample array pond;
B, laser instrument send a branch of near-infrared excitation light, and the exciting light that laser instrument is sent by conduction optical fiber is input to beam splitter, and a branch of exciting light received is divided into multi beam exciting light by beam splitter, excites each the up-conversion luminescence sample on sample array pond simultaneously;
C, infrared fileter are imaged on the photosurface of luminous signal harvester by wide-angle lens, change into fluoroscopic image after the up-conversion fluorescence signal that each up-conversion luminescence sample produces is carried out denoising;
D, mobile phone analyze and process after receiving fluoroscopic image, calculate the concentration information obtaining each up-conversion fluorescence sample.
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Cited By (15)
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CN106404733A (en) * | 2016-09-18 | 2017-02-15 | 天津博硕东创科技发展有限公司 | Mobile intelligent terminal-based food safety detection device and method |
CN107121421A (en) * | 2017-06-20 | 2017-09-01 | 内江师范学院 | Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample |
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CN108872175A (en) * | 2018-07-19 | 2018-11-23 | 湖北大学 | A kind of green fluorescence carbon quantum dot and preparation method, application |
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CN109696427A (en) * | 2018-12-28 | 2019-04-30 | 西安建筑科技大学 | A method of determining river microbe condition and selection control techniques |
CN109900894A (en) * | 2019-04-22 | 2019-06-18 | 广西埃韦迪生物科技有限公司 | A kind of hospital handheld formula fluorescence immunity analyzer |
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CN113640265A (en) * | 2021-08-11 | 2021-11-12 | 江苏大学 | Portable device based on smart mobile phone imaging detection diethylstilbestrol |
WO2024045220A1 (en) * | 2022-08-31 | 2024-03-07 | 集美大学 | Biosensing platform for in-situ sampling and target detection on basis of upconversion luminescence |
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Application publication date: 20160727 |