CN107121421A - Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample - Google Patents
Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample Download PDFInfo
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- CN107121421A CN107121421A CN201710469550.1A CN201710469550A CN107121421A CN 107121421 A CN107121421 A CN 107121421A CN 201710469550 A CN201710469550 A CN 201710469550A CN 107121421 A CN107121421 A CN 107121421A
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- 238000001514 detection method Methods 0.000 title claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 59
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 12
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- 238000003860 storage Methods 0.000 claims abstract description 60
- 238000012360 testing method Methods 0.000 claims abstract description 42
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- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 3
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6447—Fluorescence; Phosphorescence by visual observation
Abstract
The invention discloses the portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample, the large-scale detection device for solving metal ion in the prior art is not suitable for field quick detection, the problem of Test paper sensitivity is low, resultant error is big.Present invention range estimation luminoscope includes Microfluidic array chip, is embedded at the multi-colored led array of Microfluidic array chip bottom, the lightshade cover being connected with Microfluidic array chip top side detachable, and is embedded at the microoscillator of multi-colored led array bottom；Microfluidic array chip includes being used to be inlayed with multi-colored led array and basalis in the form of annular discs, and it is integrated in Microfluidic array chip body on basalis upper surface and rounded, Microfluidic array chip body is included located at the testing sample pond of its home position, with centered on testing sample pond equally distributed quantity be at least the liquid storage tank of six, testing sample pond is directly connected to six liquid storage tanks respectively by six microchannels.
Description
Technical field
The invention belongs to technical field of analysis and detection, and in particular to in Site Detection water sample heavy metal ion it is portable
Formula estimates luminoscope and method.
Background technology
The pollution of heavy metal ion is increasingly serious in life, and the health to the mankind has constituted a serious threat.Wherein, lead exists
Largely exist in environmental water sample, lead is to the harm of health it is well known that serious " lead poisoning occurs for the Hui County of Gansu in 2006
Successively " lead poisoning event " occurs for event ", the Hunan Province Wugang of 2009 and Shaanxi Province Fengxiang.Wherein Shaanxi Province Fengxiang County " lead
Poisoning " event causes children's alopecia of nearly 900 people, depauperation, intellectual impairment., Ni Niya lead contamination events in 2010
Even more cause more than 250 people dead." lead poisoning " event repeatedly occurs, and endangers startling, makes people pained.Harm of the lead to human body
It is mainly reflected in：Lead poisoning can be caused in accumulated in creatures.Studied according to the World Health Organization, enter people daily now
The lead of the body about 400 μ g from food, the μ g of water 10, the μ g of urban air 26.In addition, scientific investigations showed that lead is to blood of human body system
System, nervous system, urinary system, immunologic function etc. can damage effect.Bigger to mankind's harm is the carcinogenicity of lead,
The international cancer research associations (IARC) of inorganic Qian Yibei are classified as the carcinogenic substance of the mankind.
For the emergency monitoring of metal ion pollution accident in environmental water sample is better achieved, in order to avoid because of sudden environmental water sample
The generation repeatedly of middle lead ion poisoning；Also preferably to serve the related personnel such as environmental monitoring in the scene of the accident, utilize
Small-sized, portable, simple, fast detector or device, to the species, concentration, pollution of polluter within the time as short as possible
Scope judge, create it is a kind of can be used in lead ion contamination accident in environmental water sample it is live, determine immediately it is portable
Formula device, the control for environmental pollution burst accident is significant.
It can be used to detect that the instrument of metal ion is many at present, but there is sample handling processes complexity mostly, excessively rely on
Large-scale instrument and equipment (atomic absorption instrument etc.), consuming take, are often used for some large-scale detections such as Environmental Protection Agency, Quality Supervision Bureau
Mechanism.The harsh operating condition of these instruments causes the features such as they are without portability and general applicability, and requires
Testing staff must possess corresponding specialized technical knowledge etc..Therefore, scene, quick detection are not suitable for.There is research to report in recent years
The Test paper of road heavy metal lead ion, available for scene, but its Cleaning Principle is often based on dyestuff and heavy metal ion
Chromogenic reaction, and the sensitivity of this reaction principle heavy metal ion detection far below aggregation-induced emission principle should
With.
Therefore it provides the detector of a metal ion species, can quickly and easily be detected, and sensitivity is high, detection knot
Accurately and reliably, equipment investment is small, is easy to carry, suitable for Site Detection for fruit, becomes that those skilled in the art are urgently to be resolved hurrily to be asked
Topic.
The content of the invention
Present invention solves the technical problem that being to provide glimmering for the portable range estimation of heavy metal ion in Site Detection water sample
Light instrument, the large-scale detection device for solving metal ion in the prior art is not suitable for field quick detection, Test paper sensitivity is low,
The problem of resultant error is big.
The present invention also provides the method detected using portable range estimation luminoscope to heavy metal ion in water sample.
The technical solution adopted by the present invention is as follows：
For the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, including it is built-in with and a huge sum of money in water sample
Can occur aggregation-induced emission after category contacted with ions and form the Microfluidic array chip of the composite nano-gold substrate of aggregation, it is embedding
Located at Microfluidic array chip bottom be used for send certain wavelength exciting light and utilization this excite light irradiation aggregation to produce
The multi-colored led array of intense fluorescence, is connected with Microfluidic array chip top side detachable and is used for fluorescence produced by aggregation
The lightshade cover observed, and be embedded at multi-colored led array bottom be used for produce shake to accelerate the speed of aggreation
Microoscillator；Microfluidic array chip includes being used to be inlayed with multi-colored led array and basalis in the form of annular discs, and
Microfluidic array chip body on basalis upper surface and rounded is integrated in, Microfluidic array chip body includes being located at it
The testing sample pond of home position, and equally distributed quantity is at least the liquid storage tank of six centered on testing sample pond, treats
Sample cell is surveyed to be directly connected to six liquid storage tanks respectively by six microchannels.
Further, lightshade cover is shaped hollow round table, and its both ends open, the big opening end of lightshade cover bottom and micro-fluidic battle array
Row chip is detachably connected, and the osculum end at the top of lightshade cover is at the position of peep hole in peep hole, lightshade cover cavity
It is transversely provided with an amplifying lens.
Further, also include passing through the Zhi Wei on Microfluidic array chip body on Microfluidic array chip body
Passage, the liquid storage little Chi connected with microchannel, liquid storage little Chi quantity is more than a pair, quantity and the liquid storage little Chi of branch microchannel
Quantity it is identical and correspond.
Further, every a pair of liquid storages little Chi is symmetrically distributed in microchannel both sides, and connects same a pair of liquid storage little Chi's
A pair microchannels are equally symmetrically distributed in microchannel both sides, are equipped with microchannel with every a pair microchannels connection
One compares little Chi, and all control little Chi are equally spaced between testing sample pond and liquid storage tank.
Further, microchannel, the liquid storage tank that is connected with microchannel and all connected by branch microchannel with microchannel
Liquid storage little Chi collectively constitute a detection group on chip, chip is provided with least six detection groups.
Further, the material of basalis is glass, and chip is PDMS materials, testing sample pond, liquid storage tank, liquid storage little Chi
And control little Chi is circle.
Further, the width of microchannel and branch microchannel is 800 μm, and depth is 800 μm, and is straight channel.
The method detected using portable range estimation luminoscope to heavy metal ion in water sample, it is characterised in that：Including
Following steps：
Step 1：Different amounts of composite nano-gold substrate is put at least five liquid storage tanks, composite nano-gold will be put into
The liquid storage tank of substrate is not put into the liquid storage tank of composite nano-gold substrate as control pond as detection cell；
Step 2：After environmental water sample is filtered, testing sample pond is instilled；
Step 3：The environmental water sample in testing sample pond is instilled under the oscillation action of microoscillator, passes through microchannel respectively
Uniformly diffuse in all liquid storage tanks；With composite nano-gold substrate aggreation, generation occur for the metal ion in environmental water sample
The aggregation of fluorescence can be sent；
Step 4：As a result judge：The big opening end of lightshade cover is placed on integrated micro-fluidic array chip, covers and observes pair
According to pond and all detection cells；Under the specific wavelength light source irradiation that multi-colored led array is sent, all detection cells are compared with control pond
Compared with showing fluorescence, then contain metal ion in environmental water sample, while according to different fluorescent brightness, tentatively judging environmental water sample
The concentration of middle metal ion.
Further, metal ion is lead ion, and composite nano-gold substrate is AuNPs@(SG)X。
Compared with prior art, the device have the advantages that as follows：
The present invention can quickly and easily carry out the detection of metal ion in water sample, and sensitivity is high, as a result accurately and reliably, if
It is standby to invest small, it is easy to carry, suitable for Site Detection.
The present invention generates aggregation using metal ion with composite nano-gold substrate reactions, and the aggregation is in special wavelength light
Show fluorescence under source, so that the detection of metal ion is realized, and according to the different brightness of fluorescence, metal ion is dense in water sample in realization
Spend the judgement of size.The present invention can realize the detection of different metal ions by being put into different composite nano-gold substrates.
Sensitivity of the present invention is high, and 10 μM are can reach to lead ion minimal detectable concentration；Detection method is simple, quick, only needs
Testing result can just be gone out within several seconds；Easy to operate, the people without specialty chemical knowledge can also be judged by result color contrast
Concentration；Equipment is miniaturized, and example invests small in carrying, supporting less demanding, only needs syringe to match miillpore filter.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention.
Fig. 2 is chip structure schematic diagram of the present invention.
Fig. 3 is Pb2+With the UV absorption and fluorescence spectra of solution after composite nano-gold substrate reactions.
Wherein, reference is corresponding entitled：1- microoscillators, 2- multi-colored led arrays, 3- micro-fluidic array cores
Piece, 4- lightshade covers, 5- chips, 6- testing samples pond, 7- liquid storage tanks, 8- microchannels, 9- liquid storages little Chi, 10- branch microchannel, 11-
Compare little Chi, 12- amplifying lenses, 13- peep holes, 14- basalises.
Embodiment
The invention will be further described with embodiment for explanation below in conjunction with the accompanying drawings, and mode of the invention includes but not only limited
In following examples.
Embodiment 1
As illustrated in fig. 1 and 2, the portable range estimation luminoscope for heavy metal ion in Site Detection water sample, including built-in
Have with aggregation-induced emission can occur after heavy metal contact in water sample and formed aggregation composite nano-gold substrate it is micro-fluidic
Array chip 3, be embedded at the bottom of Microfluidic array chip 3 be used for send certain wavelength exciting light and utilization this excite
Aggregation described in light irradiation is to produce the multi-colored led array 2 of intense fluorescence, with the top side detachable of Microfluidic array chip 3
The lightshade cover 4 observed fluorescence produced by the aggregation is connected and be used for, and is embedded at the multi-colored led array 2
Bottom is used to produce concussion with the microoscillator 1 for the speed for accelerating the aggreation；The Microfluidic array chip 3 includes
For being inlayed with the multi-colored led array 2 and basalis 14 in the form of annular discs, and it is integrated in the upper end of basalis 14
On face and rounded Microfluidic array chip body 5, the Microfluidic array chip body 5 is included located at its home position
Testing sample pond 6, and equally distributed quantity is at least the liquid storage tank 7 of six centered on the testing sample pond 6, it is described to treat
Sample cell 6 is surveyed to be directly connected to six liquid storage tanks 7 respectively by six microchannels 8.
In order to preferably observe the fluorescence that the aggregation is produced, lightshade cover 4 is set to shaped hollow round table, and its two ends is opened
Mouthful, the big opening end of the bottom of lightshade cover 4 is detachably connected with Microfluidic array chip 3, the osculum end at the top of lightshade cover 4
For peep hole 13, the cavity of lightshade cover 4 is interior to be transversely provided with an amplifying lens 12 at the position of peep hole 13.
In order to preferably observe the fluorescence that the aggregation is produced, it is ensured that testing result it is accurate, set blank control and
The detection group of parallel laboratory test can be carried out to reduce error.Also include by being located on the Microfluidic array chip body 5
The liquid storage little Chi 9 that branch microchannel 10 on the Microfluidic array chip body 5 is connected with the microchannel 8, the liquid storage is small
The quantity in pond 9 is more than a pair, and the quantity of the branch microchannel 10 is identical with the quantity of the liquid storage little Chi 9 and one a pair
Should.Liquid storage little Chi 9 described in every a pair is symmetrically distributed in the both sides of microchannel 8, and connects with liquid storage little Chi's 9 described in a pair
A pair microchannels 10 are equally symmetrically distributed in the both sides of microchannel 8, with Zhi Weitong described in every a pair on the microchannel 8
The connection of road 10 is equipped with one and compares little Chi 11, and all control little Chi 11 are equally spaced in the testing sample pond 6
Between liquid storage tank 7.The microchannel 8, the liquid storage tank 7 connected with the microchannel 8 and all pass through the branch microchannel
The detection that the 10 liquid storage little Chi 9 connected with the microchannel 8 are collectively constituted on the Microfluidic array chip body 5 is small
Group, the Microfluidic array chip body 5 is provided with least six detection groups.
The control little Chi 11 is arranged on the junction of microchannel 8 and branch microchannel 10, can accelerate sample from microchannel 8
Diffuse in liquid storage little Chi 9, accelerate reaction speed.
In order to ensure that what multi-colored led array sent excites light-transmissive basalis 14 to irradiate aggregation, the basalis
14 material is glass, and the chip 5 is PDMS materials, the testing sample pond 6, liquid storage tank 7, liquid storage little Chi 9 and control
Little Chi 11 is circle.
It is a kind of the macromolecule organic silicon compound by dimethyl silicone polymer Polydimethylsiloxane, PDMS, tool
The features such as having that nontoxicity, highly-breathable, translucency are good, biocompatibility is good, easily engaged with various material room temperature.In addition, its
Cost is low, using simple, and has splendid plasticity, heat endurance and chemical inertness, can be used for multiple times after ultrasonic cleaning.This hair
Open-birth into aggregation be dissolved in water, complete after detection, the Microfluidic array chip 3 dismantled down, is available for down after ultrasonic cleaning
It is secondary to use.
In order to ensure the quick of reaction, sampling is less and acquisition optimum response is matched, the microchannel 8 and branch microchannel 10
Width is 800 μm, and depth is 800 μm, and is straight channel.
Embodiment 2
The method detected using Portable fluorescence instrument to heavy metal ion in water sample, is comprised the following steps：
Comprise the following steps：
Step 1：In five liquid storage tanks 7 as detection cell, the composite nano-gold bottom of same volume various concentrations is respectively put into
Thing solution, a remaining liquid storage tank 7 does not place composite nano-gold substrate solution as control pond.
Step 2：After environmental water sample is filtered, the testing sample pond 6 is instilled.
Step 3：The environmental water sample in testing sample pond 6 is instilled under the oscillation action of microoscillator 1, respectively by micro- logical
Road 8 is uniformly diffused in all liquid storage tanks 7；With the composite nano-gold substrate aggregation occurs for the metal ion in environmental water sample instead
Should, generation can send the aggregation of fluorescence.
Step 4：As a result judge：The big opening end of the lightshade cover 4 is placed on integrated micro-fluidic array chip 3, covering pair
According to pond and all detection cells；Naked eyes observe fluorescing matter by peep hole 13 and amplifying lens 12.In the multi-colored led array 2
Under the specific wavelength light source irradiation sent, all detection cells compare with control pond, show fluorescence, then contain gold in environmental water sample
Belong to ion, while according to different fluorescent brightness, the preliminary concentration for judging metal ion in environmental water sample.
Embodiment 3
The method detected using Portable fluorescence instrument to heavy metal ion in water sample, is comprised the following steps：
Step 1：Using six liquid storage tanks 7 as detection cell, the composite nano-gold substrate for being put into same volume various concentrations is molten
Liquid；Liquid storage little Chi 9 is not put into composite nano-gold substrate solution with compareing little Chi 11 as control pond.
Step 2：After environmental water sample is filtered, the testing sample pond 6 is instilled.
Step 3：The environmental water sample in testing sample pond 6 is instilled under the oscillation action of microoscillator 1, respectively by micro- logical
Road 8 is uniformly diffused in all liquid storage tanks 7；With composite nano-gold substrate aggreation occurs for the metal ion in environmental water sample,
Generation can send the aggregation of fluorescence.
Step 4：As a result judge：The big opening end of the lightshade cover 4 is placed on integrated micro-fluidic array chip 3, covering pair
According to pond and all detection cells；Naked eyes observe fluorescing matter by peep hole 13 and amplifying lens 12.In the multi-colored led array 2
Under the specific wavelength light source irradiation sent, all detection cells compare with control pond, show fluorescence, then contain gold in environmental water sample
Belong to ion, while according to different fluorescent brightness, the preliminary concentration for judging metal ion in environmental water sample.
Embodiment 4
The method detected using Portable fluorescence instrument to heavy metal ion in water sample, is comprised the following steps：
Step 1：The liquid storage tank 7 that is connected by a microchannel 8, with the microchannel 8 and it is all by branch microchannel 10 with
The liquid storage little Chi 9 of the microchannel 8 connection is collectively as a detection group.By the liquid storage tank 7 in detection group, liquid storage little Chi 9
As detection cell, the composite nano-gold substrate solution of same volume various concentrations is respectively put into；Control in detection group is small
Pond 11 is not put into composite nano-gold substrate solution as control pond.
Microfluidic array chip body 5 carries out six groups of parallel tests provided with six detection groups.
Step 2：After environmental water sample is filtered, the testing sample pond 6 is instilled.
Step 3：The environmental water sample in testing sample pond 6 is instilled under the oscillation action of microoscillator 1, respectively by micro- logical
Road 8 is uniformly diffused in all liquid storage tanks 7, liquid storage little Chi 9 and control little Chi 11；Metal ion in environmental water sample is received with compound
Aggreation occurs for the golden substrate of rice, and generation can send the aggregation of fluorescence.
Step 4：As a result judge：The big opening end of the lightshade cover 4 is placed on integrated micro-fluidic array chip 3, covering pair
According to pond and all detection cells；Naked eyes observe fluorescing matter by peep hole 13 and amplifying lens 12.In the multi-colored led array 2
Under the specific wavelength light source irradiation sent, all detection cells compare with control pond, show fluorescence, then contain gold in environmental water sample
Belong to ion, while according to different fluorescent brightness, the preliminary concentration for judging metal ion in environmental water sample.
By setting six groups of parallel tests, reduce test error, it is ensured that the accuracy of testing result.Sensitivity is higher, right
Lead ion minimal detectable concentration can reach 10 μM.
Embodiment 5
Composite nano-gold substrate is AuNPs@(SG)XSpecific preparation process it is as follows：
1) by 1.0mL, concentration is 10mmol/L HAuCl4The aqueous solution and 1.0mL, concentration are 25mmol/L GSH solution
Mixing, adds 5mL ultra-pure water, in slowly stirring 15min under 200rpm rotating speed at a temperature of 30 DEG C, forms cotton-shaped heavy
Form sediment；
2) pH of above-mentioned mixed solution is adjusted to 5.5, is completely dissolved precipitation, solution is changed into clear；
3) after aging 2h, the pH to 7.5 of regulation mixed solution can obtain composite nano-gold substrate for AuNPs@(SG)X。
Composite nano-gold substrate A uNPs@(SG) manufactured in the present embodimentXShow the transparent aqueous solution of achromaticity and clarification.
Embodiment 6
Comparative example
Lead ion in water sample is determined using lead ion test paper.
The preparation of sample：Ultra-pure water and a small bay in a river river are taken, different amounts of plumbi nitras is added respectively, being configured to plumbum ion concentration is
0mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L aqueous solution and a small bay in a river river solution.
Detection method：The lead Test paper inspection for being 0-500mg/L with inspection range by the above-mentioned aqueous solution and a small bay in a river river solution
Survey.By sample drop on Test paper, observe the color change of test paper, and compared with colorimetric card, thus draw in sample lead from
Sub- concentration.
As a result show, the detection sensitivity of Test paper is relatively low, and concentration gradient is not in the color range on colorimetric card
Visual larger error is easily caused when substantially, less than 400mg/L.
Embodiment 7
Comparative example
Lead ion in water sample is determined using colorimetric cylinder detection method.
Detection sample is used as using the sample in embodiment 5.
Detection method：The lead ratio colour tube detection for being 0-3mg/L with inspection range by the above-mentioned aqueous solution and a small bay in a river river solution, will
The color of reacted lead ratio colour tube is compared with colorimetric card.
As a result show, the detection sensitivity of colorimetric cylinder is relatively low, and concentration gradient is failed to understand in the color range on colorimetric card
It is aobvious, visual larger error is easily caused during less than 400mg/L.
The present invention generates aggregation using metal ion with composite nano-gold substrate reactions, and the aggregation is in special wavelength light
Show fluorescence under source, so that the detection of metal ion is realized, and according to the different brightness of fluorescence, metal ion is dense in water sample in realization
Spend the judgement of size.The present invention can realize the detection of different metal ions by being put into different composite nano-gold substrates.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, it is noted that all
Any modifications, equivalent substitutions and improvements made within the spirit and principles in the present invention etc., should be included in the guarantor of the present invention
Within the scope of shield.
Claims (9)
1. the portable range estimation luminoscope for heavy metal ion in Site Detection water sample, it is characterised in that：Including be built-in with
Can occur aggregation-induced emission after heavy metal ion contact in water sample and form the micro-fluidic of the composite nano-gold substrate of aggregation
Array chip (3), is embedded at the Microfluidic array chip (3) bottom and is somebody's turn to do for sending the exciting light of certain wavelength and utilizing
Aggregation described in light irradiation is excited to produce the multi-colored led array (2) of intense fluorescence, is pushed up with the Microfluidic array chip (3)
Portion is detachably connected and is used for the lightshade cover (4) observed fluorescence produced by the aggregation, and is embedded at described many
Color LED array (2) bottom is used to produce concussion with the microoscillator (1) for the speed for accelerating the aggreation；The miniflow
Control array chip (3) includes being used to be inlayed with the multi-colored led array (2) and basalis (14) in the form of annular discs, and
It is integrated in Microfluidic array chip body (5) on the basalis (14) upper surface and rounded, the micro-fluidic array core
Piece body (5) is included located at the testing sample pond (6) of its home position, and is uniformly divided centered on the testing sample pond (6)
The quantity of cloth is at least the liquid storage tank (7) of six, and the testing sample pond (6) is stored up with six respectively by six microchannels (8)
Liquid pool (7) is directly connected to.
2. according to claim 1 be used for the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, it is special
Levy and be：The lightshade cover (4) is shaped hollow round table, and its both ends open, the big opening end and miniflow of lightshade cover (4) bottom
Control array chip (3) is detachably connected, and the osculum end at the top of the lightshade cover (4) is peep hole (13), and the lightshade cover (4) is empty
Intracavitary is being transversely provided with an amplifying lens (12) at the position of peep hole (13).
3. according to claim 2 be used for the Portable fluorescence instrument of heavy metal ion in Site Detection water sample, its feature exists
In：Also include passing through the branch microchannel on Microfluidic array chip body (5) on the Microfluidic array chip body (5)
(10) the liquid storage little Chi (9), connected with the microchannel (8), the quantity of the liquid storage little Chi (9) is more than a pair, the branch
The quantity of microchannel (10) is identical with the quantity of the liquid storage little Chi (9) and corresponds.
4. according to claim 3 be used for the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, it is special
Levy and be：Liquid storage little Chi (9) described in every a pair is symmetrically distributed in the microchannel (8) both sides, and connects with storage described in a pair
Liquid little Chi (9) a pair microchannels (10) are equally symmetrically distributed in the microchannel (8) both sides, on the microchannel (8)
One, which is equipped with, with branch microchannel (10) connection described in every a pair compares little Chi (11), all control little Chi (11) etc.
Away from being distributed between the testing sample pond (6) and liquid storage tank (7).
5. according to claim 4 be used for the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, it is special
Levy and be：The microchannel (8), the liquid storage tank (7) connected with the microchannel (8) and all by the branch microchannel
(10) the liquid storage little Chi (9) connected with the microchannel (8) collectively constitutes a detection group on the chip (5), described
Chip (5) is provided with least six detection groups.
6. according to claim 5 be used for the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, it is special
Levy and be：The material of the basalis (14) is glass, and the chip (5) is PDMS materials, the testing sample pond (6), storage
Liquid pool (7), liquid storage little Chi (9) and control little Chi (11) are circle.
7. according to claim 6 be used for the portable range estimation luminoscope of heavy metal ion in Site Detection water sample, it is special
Levy and be：The width of the microchannel (8) and branch microchannel (10) is 800 μm, and depth is 800 μm, and is straight channel.
8. heavy metal ion in water sample is examined using the portable range estimation luminoscope as described in claim 1-7 any one
The method of survey, it is characterised in that：Comprise the following steps：
Step 1：Different amounts of composite nano-gold substrate is put into liquid storage tank described at least five (7), compound receive will be put into
The liquid storage tank (7) of the golden substrate of rice is not put into the liquid storage tank (7) of composite nano-gold substrate as control pond as detection cell；
Step 2：After environmental water sample is filtered, the testing sample pond (6) is instilled；
Step 3：The environmental water sample of testing sample pond (6) is instilled under the oscillation action of microoscillator (1), respectively by micro- logical
Road (8) is uniformly diffused in all liquid storage tanks (7)；Metal ion in environmental water sample occurs poly- with the composite nano-gold substrate
Collection reaction, generation can send the aggregation of fluorescence；
Step 4：As a result judge：The big opening end of the lightshade cover (4) is placed on integrated micro-fluidic array chip (3), covered simultaneously
Observation control pond and all detection cells；Under the specific wavelength light source irradiation that the multi-colored led array (2) is sent, all detections
Pond compares with control pond, shows fluorescence, then contains metal ion in environmental water sample, while according to different fluorescent brightness, just
Step judges the concentration of metal ion in environmental water sample.
9. method according to claim 8, it is characterised in that：The metal ion is lead ion, the composite nano-gold
Substrate is AuNPs@(SG)X。
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