CN106018718A - Multi-component food safety detection microfluidic chip - Google Patents
Multi-component food safety detection microfluidic chip Download PDFInfo
- Publication number
- CN106018718A CN106018718A CN201610580749.7A CN201610580749A CN106018718A CN 106018718 A CN106018718 A CN 106018718A CN 201610580749 A CN201610580749 A CN 201610580749A CN 106018718 A CN106018718 A CN 106018718A
- Authority
- CN
- China
- Prior art keywords
- food safety
- safety detection
- reagent storage
- fluidic chip
- pool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
Landscapes
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multi-component food safety detection microfluidic chip, comprising a sample pool (1) and 2-8 micro channels; each micro channel includes an inflow channel (2), a reagent storage pool (3), a detection channel (4), a detection chamber (5), an outflow channel (6) and a waste liquid pool (7), wherein the sample pool (1) is connected and fitted to the reagent storage pool (3) through the inflow channel (2), the reagent storage pool (3) is connected and fitted to the detection chamber (5) through the detection channel (4), the detection chamber (5) is connected and fitted to the waste liquid pool (7) through the outflow channel (6), and an included angle between each of the micro channels is 45-180 degrees. The multi-component food safety detection microfluidic chip provided herein is convenient to operate, short in detection time and low in sample consumption and enables simultaneous detection for multiple components with no need repeated sample adding.
Description
Technical field
The present invention relates to microfluidic chip analysis and food safety field of fast detection, be specifically related to a kind of multicomponent food safety detection micro-fluidic chip.
Background technology
With economic fast development, various agricultural product the most extensively flow, and food safety hidden danger the most repeatedly occur, and people in the urgent need to identifying the harmful components in food by advanced Fast Detection Technique, thus provides safeguard for the healthy of people.Micro-fluidic chip be a kind of high flux, sample consume less, simple and quick novel detection technique platform, this platform can by sample pretreatment, react, separate and detect process integration in chip piece, it is automatically performed whole analysis process, is widely deployed in food safety detection.
The colloidal gold fast detecting test paper strip flux being conventionally used to food safety detection is little, every kind of test strips correspondence detection one detection material, detects then complex operation for Multiple components, it is impossible to accomplish once to be loaded detection Multiple components.
Summary of the invention
Instant invention overcomes above-mentioned shortcoming, and a kind of multicomponent food safety detection micro-fluidic chip is provided.
To achieve these goals, the present invention is to realize by the following technical solutions: this multicomponent this chip of food safety detection micro-fluidic chip includes sample cell and 2 ~ 8 microchannels, every microchannel includes flow channel, reagent storage pool, sense channel, sensing chamber, flow pass and waste liquid pool, wherein, sample cell is connected installation by flow channel with reagent storage pool, reagent storage pool is connected by sense channel and sensing chamber to be installed, sensing chamber is connected by flow pass and waste liquid pool installs, and the angle between described every microchannel is 45 ° ~ 180 °；The quantity of described sample cell is 1；The quantity of described flow channel is 2 ~ 8；The quantity of described reagent storage pool is 2 ~ 8；The quantity of described sense channel is 2 ~ 8；The quantity of described sensing chamber is 2 ~ 8；The quantity of described flow pass is 2 ~ 8；The quantity of described waste liquid pool is 2 ~ 8；Described multicomponent food safety detection micro-fluidic chip is disc-shaped.
In described sample cell, sample pad is set.
Preferably, described sample pad is glass fibre element film.
Described reagent storage pool inner surface cover glass fiber, wherein, fiberglass surfacing is coated with biochemical reagents.
Antigen, enzyme or developer is stored in described sensing chamber.
Preferably, the lateral cross of described reagent storage pool is any one in polygon or circle.
Preferably, any one during the lateral cross of described sensing chamber is polygon or circle.
Preferably, any one during the lateral cross of described waste liquid pool is polygon or circle.
The invention has the beneficial effects as follows: (1) scheme is complete, easy to operate；(2) the detection time is short；(3) sample consumption is few；(4) Multiple components can be detected without sample-adding is repeated several times simultaneously.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
The implication of reference in figure, 1, sample cell, 2, flow channel, 3, reagent storage pool, 4, sense channel, 5, sensing chamber, 6, flow pass, 7, waste liquid pool.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with detailed description of the invention, the present invention is expanded on further.
This multicomponent food safety detection micro-fluidic chip is to achieve these goals, the present invention is to realize by the following technical solutions: this multicomponent this chip of food safety detection micro-fluidic chip includes sample cell 1 and 2 ~ 8 microchannels, every microchannel includes flow channel 2, reagent storage pool 3, sense channel 4, sensing chamber 5, flow pass 6 and waste liquid pool 7, wherein, sample cell 1 is connected installation by flow channel 2 with reagent storage pool 3, reagent storage pool 3 is connected by sense channel 4 and sensing chamber 5 to be installed, sensing chamber 5 is connected by flow pass 6 and waste liquid pool 7 installs, angle between every microchannel is 60 °；The quantity of sample cell 1 is 1；The quantity of flow channel 2 is 6；The quantity of reagent storage pool 3 is 6；The quantity of sense channel 4 is 6；The quantity of sensing chamber 5 is 6；The quantity of flow pass 6 is 6；The quantity of waste liquid pool 7 is 6；Multicomponent food safety detection micro-fluidic chip is disc-shaped；In sample cell 1, sample pad is set；Sample pad is Pilus Caprae seu Ovis felt pan；Reagent storage pool 3 inner surface cover glass fiber, wherein, fiberglass surfacing is coated with biochemical reagents；Storage antigen, enzyme or developer in sensing chamber 5；The lateral cross of reagent storage pool 3 is circular；The lateral cross of sensing chamber 5 is circular；The lateral cross of waste liquid pool 7 is circular.
During detection, testing sample is added sample cell 1, be lined with sample pad in sample cell for filtering.By hydraulic pressure difference, in sample cell, liquid is driven into flow channel 2, liquid enters in reagent storage pool 3.Fluid enters sensing chamber 5 by sense channel 4 subsequently, is changed by naked-eye observation sensing chamber color, and liquid flows into waste liquid pool 7 eventually through flowing out flow pass 6.The gold colloidal glass fibre being sprayed with antibody labeling, sensing chamber 5 internal labeling antigen is stored in advance in reagent storage pool 3.When in liquid to be detected containing detection subject molecule time, 5 endoantigens can not capture antibody labeling gold colloidal detection, 5 present colourless；When not containing detection subject molecule in liquid to be detected, 5 endoantigens can capture the gold colloidal of antibody labeling so that 5 present claret.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; the principle that the present invention is simply described described in above-described embodiment and description; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within scope of the claimed invention.Claimed scope is defined by appending claims and equivalent thereof.
Claims (8)
1. a multicomponent food safety detection micro-fluidic chip, it is characterized in that, this chip includes sample cell (1) and 2 ~ 8 microchannels, every microchannel includes flow channel (2), reagent storage pool (3), sense channel (4), sensing chamber (5), flow pass (6) and waste liquid pool (7), wherein, sample cell (1) is connected installation by flow channel (2) with reagent storage pool (3), reagent storage pool (3) is connected by sense channel (4) and sensing chamber (5) to be installed, sensing chamber (5) is connected by flow pass (6) and waste liquid pool (7) installs, angle between described every microchannel is 45 ° ~ 180 °；The quantity of described sample cell (1) is 1；The quantity of described flow channel (2) is 2 ~ 8；The quantity of described reagent storage pool (3) is 2 ~ 8；The quantity of described sense channel (4) is 2 ~ 8；The quantity of described sensing chamber (5) is 2 ~ 8；The quantity of described flow pass (6) is 2 ~ 8；The quantity of described waste liquid pool (7) is 2 ~ 8；Described multicomponent food safety detection micro-fluidic chip is disc-shaped.
2. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that described sample cell arranges sample pad in (1).
3. according to the multicomponent food safety detection micro-fluidic chip described in claim 1 or 2, it is characterised in that described sample pad is glass fibre element film.
4. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that described reagent storage pool (3) inner surface cover glass fiber, wherein, fiberglass surfacing is coated with biochemical reagents.
5. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that storage antigen, enzyme or developer in described sensing chamber (5).
6. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that the lateral cross of described reagent storage pool (3) is any one in polygon or circle.
7. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that the lateral cross of described sensing chamber (5) is any one in polygon or circle.
8. according to the multicomponent food safety detection micro-fluidic chip described in claim 1, it is characterised in that the lateral cross of described waste liquid pool (7) is any one in polygon or circle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610580749.7A CN106018718A (en) | 2016-07-21 | 2016-07-21 | Multi-component food safety detection microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610580749.7A CN106018718A (en) | 2016-07-21 | 2016-07-21 | Multi-component food safety detection microfluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106018718A true CN106018718A (en) | 2016-10-12 |
Family
ID=57116244
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610580749.7A Pending CN106018718A (en) | 2016-07-21 | 2016-07-21 | Multi-component food safety detection microfluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106018718A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107121421A (en) * | 2017-06-20 | 2017-09-01 | 内江师范学院 | Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample |
| CN108554466A (en) * | 2018-03-01 | 2018-09-21 | 深圳韦尔达科技合伙企业（有限合伙） | The centrifugal separating device of micro-fluidic chip |
| CN109444095A (en) * | 2018-10-17 | 2019-03-08 | 重庆城市管理职业学院 | The residual rapid detection system of double-photo signal trace agriculture |
| CN113134400A (en) * | 2021-05-22 | 2021-07-20 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
| CN114839179A (en) * | 2022-04-26 | 2022-08-02 | 华中农业大学 | Method for rapidly detecting organochlorine pesticide residues in plant matrix |
| CN113134400B (en) * | 2021-05-22 | 2024-11-12 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121450A1 (en) * | 2002-12-19 | 2004-06-24 | Pugia Michael J. | Method and apparatus for splitting of specimens into multiple channels of a microfluidic device |
| JP2011196849A (en) * | 2010-03-19 | 2011-10-06 | Rohm Co Ltd | Rotating analysis chip and measurement system using the same |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
| CN103323605A (en) * | 2013-06-18 | 2013-09-25 | 杭州普施康生物科技有限公司 | Micro-fluidic chip for glycosylated hemoglobin immunodetection |
| CN103389371A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Disc-type multi-index analysis chip |
| CN103808948A (en) * | 2014-03-12 | 2014-05-21 | 杭州霆科生物科技有限公司 | Micro-fluidic chip system and method for pesticide residue field detection |
| CN103831140A (en) * | 2014-03-07 | 2014-06-04 | 博奥生物集团有限公司 | Micro-fluidic chip for multi-index detection |
| CN204514801U (en) * | 2015-04-01 | 2015-07-29 | 杭州霆科生物科技有限公司 | A kind of throwing type micro-fluidic chip for residues of pesticides Site Detection |
| CN205120593U (en) * | 2015-11-25 | 2016-03-30 | 深圳出入境检验检疫局食品检验检疫技术中心 | A micro -fluidic chip that is arranged in multiple harm factor synchronous detection of food |
| CN105572351A (en) * | 2015-12-21 | 2016-05-11 | 丹娜（天津）生物科技有限公司 | Fungus detection microfluidic chip on basis of electrochemiluminescence |
| CN205374460U (en) * | 2015-12-18 | 2016-07-06 | 德康润生物科技（天津）有限公司 | Colloidal gold immunoassay detection dish |
-
2016
- 2016-07-21 CN CN201610580749.7A patent/CN106018718A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121450A1 (en) * | 2002-12-19 | 2004-06-24 | Pugia Michael J. | Method and apparatus for splitting of specimens into multiple channels of a microfluidic device |
| JP2011196849A (en) * | 2010-03-19 | 2011-10-06 | Rohm Co Ltd | Rotating analysis chip and measurement system using the same |
| CN102784671A (en) * | 2012-08-13 | 2012-11-21 | 苏州汶颢芯片科技有限公司 | Centrifugal micro-fluidic chip for detecting pesticide residue and preparation method thereof |
| CN103323605A (en) * | 2013-06-18 | 2013-09-25 | 杭州普施康生物科技有限公司 | Micro-fluidic chip for glycosylated hemoglobin immunodetection |
| CN103389371A (en) * | 2013-08-07 | 2013-11-13 | 苏州扬清芯片科技有限公司 | Disc-type multi-index analysis chip |
| CN103831140A (en) * | 2014-03-07 | 2014-06-04 | 博奥生物集团有限公司 | Micro-fluidic chip for multi-index detection |
| CN103808948A (en) * | 2014-03-12 | 2014-05-21 | 杭州霆科生物科技有限公司 | Micro-fluidic chip system and method for pesticide residue field detection |
| CN204514801U (en) * | 2015-04-01 | 2015-07-29 | 杭州霆科生物科技有限公司 | A kind of throwing type micro-fluidic chip for residues of pesticides Site Detection |
| CN205120593U (en) * | 2015-11-25 | 2016-03-30 | 深圳出入境检验检疫局食品检验检疫技术中心 | A micro -fluidic chip that is arranged in multiple harm factor synchronous detection of food |
| CN205374460U (en) * | 2015-12-18 | 2016-07-06 | 德康润生物科技（天津）有限公司 | Colloidal gold immunoassay detection dish |
| CN105572351A (en) * | 2015-12-21 | 2016-05-11 | 丹娜（天津）生物科技有限公司 | Fungus detection microfluidic chip on basis of electrochemiluminescence |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107121421A (en) * | 2017-06-20 | 2017-09-01 | 内江师范学院 | Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample |
| CN108554466A (en) * | 2018-03-01 | 2018-09-21 | 深圳韦尔达科技合伙企业（有限合伙） | The centrifugal separating device of micro-fluidic chip |
| CN109444095A (en) * | 2018-10-17 | 2019-03-08 | 重庆城市管理职业学院 | The residual rapid detection system of double-photo signal trace agriculture |
| CN113134400A (en) * | 2021-05-22 | 2021-07-20 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
| CN113134400B (en) * | 2021-05-22 | 2024-11-12 | 杭州霆科生物科技有限公司 | Micro-fluidic chip capable of removing bubbles |
| CN114839179A (en) * | 2022-04-26 | 2022-08-02 | 华中农业大学 | Method for rapidly detecting organochlorine pesticide residues in plant matrix |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2022200683A8 (en) | Microfluidic devices having isolation pens and methods of testing biological micro-objects with same | |
| CN106018718A (en) | Multi-component food safety detection microfluidic chip | |
| Miyazaki et al. | Label-free, spatially multiplexed SPR detection of immunoassays on a highly integrated centrifugal Lab-on-a-Disc platform | |
| CN104849111B (en) | The forming method of gradient microlayer model array based on sequential injection and microflow control technique | |
| US10877032B2 (en) | Method and system of microfluidic immunoassay using magnetic beads | |
| US20100269579A1 (en) | Detecting gas compounds for downhole fluid analysis using microfluidics and reagent with optical signature | |
| MX2012005951A (en) | Fluid mixing and delivery in microfluidic systems. | |
| EP2620760A2 (en) | Microfluidic device and control method thereof | |
| WO2007002580A3 (en) | Diagnostic as say system with multi -well reagent container | |
| JP2004226403A (en) | Protein detecting flow system and protein detecting method | |
| HK1095879A1 (en) | Device and method for simultaneously carrying out blood group determination, serum cross-check and antibody detection test | |
| JP2018531369A5 (en) | ||
| CN113528625A (en) | Microfluidic nucleic acid detection method and application | |
| KR20180030108A (en) | Fluid systems for conducting analyzes | |
| CN105142790B (en) | The method and corresponding test system of micro fluidic device are filled by distribution system | |
| US20140171343A1 (en) | Biological detecting chip | |
| Hsieh et al. | High-throughput on-line multi-detection for refractive index, velocity, size, and concentration measurements of micro-two-phase flow using optical microfibers | |
| CN215783449U (en) | Self-driven micro-fluid detection card capable of controlling flow rate | |
| CN109765391B (en) | Multi-index detection centrifugal test strip chip | |
| KR102040376B1 (en) | Immunoassay kit and immunoassay method using the same | |
| CN111068798A (en) | Micro-fluidic chip with micro-porous membrane for intercepting aggregated microspheres and detection method thereof | |
| JP2010078402A (en) | Microchip | |
| Samborski et al. | Blood diagnostics using sedimentation to extract plasma on a fully integrated point‐of‐care microfluidic system | |
| KR101821410B1 (en) | Microfluidic device, method for controlling the same and method for controlling bubble | |
| CA2953755A1 (en) | Capsule for rapid molecular quantification of a fluid sample such as whole blood |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161012 |
|
| RJ01 | Rejection of invention patent application after publication |