CN109932316A - Gene sequencing Optical devices - Google Patents
Gene sequencing Optical devices Download PDFInfo
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- CN109932316A CN109932316A CN201711365301.4A CN201711365301A CN109932316A CN 109932316 A CN109932316 A CN 109932316A CN 201711365301 A CN201711365301 A CN 201711365301A CN 109932316 A CN109932316 A CN 109932316A
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- microcobjective
- laser
- optical devices
- gene sequencing
- lighting module
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Abstract
The invention discloses a kind of gene sequencing Optical devices, comprising: light source, transmitting terminal are arranged upward, for emitting laser vertically upward；Lighting module is mounted in the optical path of the light source transmitting laser, for will irradiate the test sample being located above after laser alignment；Microcobjective is mounted on the surface of the lighting module, is irradiated with a laser the fluorescence that excitation generates for collecting test sample；Detector is mounted on the focal position of the microcobjective transmission fluorescence, for obtaining fluorescence and the imaging of the microcobjective transmission.This gene sequencing Optical devices are located on a vertical straight line, other optical modules such as lens barrel module, spectral module are not needed, therefore this gene sequencing structure of optical means is compact, simple, reduce complexity, it can be realized miniaturization, and entire Optical devices are located in a straight line, and adjustment requires to significantly reduce.
Description
Technical field
The present invention relates to gene sequencing technology fields, and in particular to a kind of gene sequencing Optical devices.
Background technique
Second generation gene sequencer need by LASER Excited Fluorescence carry out base detection, Optical devices include light source,
Lighting module, microcobjective, cylinder mirror module, spectral module, detector etc..Gene sequencer Optical devices generally have higher multiple
Miscellaneous degree, illumination microscope group, which homogenizes laser shaping to transmit by microcobjective, uniformly holds in the mouth according to test sample, microcobjective and cylinder mirror
It is connected into as to detector, existing gene sequencing structure of optical means is complicated, it is more difficult to realize miniaturization.
Summary of the invention
The application provides a kind of gene sequencing Optical devices of miniaturization.
A kind of gene sequencing Optical devices are provided in a kind of embodiment, comprising:
Light source, transmitting terminal are arranged upward, for emitting laser vertically upward；
Lighting module is mounted in the optical path of the light source transmitting laser, for irradiation after laser alignment to be located above
Test sample；
Microcobjective is mounted on the surface of the lighting module, is irradiated with a laser excitation production for collecting test sample
Raw fluorescence；
Detector is mounted on the focal position of the microcobjective transmission fluorescence, for obtaining the microcobjective transmission
Fluorescence and imaging.
Further, microcobjective is the microcobjective of both sides' finite conjugate.
Further, the filter for ending laser is equipped in microcobjective.
Further, lighting module is collimating mirror.
Further, the light-emitting window of lighting module is provided with filter.
Further, light source is the laser generator for emitting one or more wavelength lasers.
Further, gene sequencing Optical devices further include the transparent platform for placing test sample, the transparent platform position
Between the lighting module and microcobjective.
According to the gene sequencing Optical devices of above-described embodiment, since light source is located at below test sample, to test sample
Transmission-type illumination excitation is carried out, to only need to be arranged in the optical path of light source a lighting module for collimation laser i.e.
Can, and microcobjective for collecting fluorescence imaging and detector are successively set on above lighting module, i.e., in test sample
Side does not need other light such as lens barrel module, spectral module so that this gene sequencing Optical devices are located on a vertical straight line
Module is learned, therefore this gene sequencing structure of optical means is compact, simple, reduces complexity, can be realized miniaturization, and entire
Optical devices are located in a straight line, and adjustment requires to significantly reduce.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of gene sequencing Optical devices in embodiment.
Specific embodiment
Below by specific embodiment combination attached drawing, invention is further described in detail.
A kind of gene sequencing Optical devices are provided in the present embodiment, this gene sequencing Optical devices are that miniaturization is set
It is standby, it is sequenced for the gene to label.
As shown in Figure 1, the gene sequencing Optical devices of the present embodiment mainly include light source 1, lighting module 2, microcobjective 3
With detector 4.Light source 1, lighting module 2, microcobjective 3 and detector 4 are arranged point-blank from bottom to top, i.e. setting exists
Light source 1 emits on the optical axis of laser.When test, test sample 5 is placed between lighting module 2 and microcobjective 3, the present embodiment
In, a transparent platform 6 is equipped with for carrying and places test sample 5, and transparent platform 6 can be transparent glass, be mounted on 2 He of lighting module
Between microcobjective 3.
Specifically, light source 1 is the laser generator for emitting a kind of wavelength or multi-wavelength's laser, the transmitting terminal court of light source 1
Upper setting.When light source 1 is the laser generator for emitting a kind of wavelength laser, this gene sequencing Optical devices are for realizing to one
Kind base detection, gene sequencing can more cooperations realizations；When light source 1 is the laser generator of multi-wavelength's laser, such as can
Emit the laser generator of two kinds of wavelength simultaneously, two kinds of bases can be detected simultaneously, thus this gene sequencing Optical devices
It can carry out timesharing base detection.
Lighting module 2 is collimating mirror, and the laser for emitting light source 1 is homogenized collimation, the laser after the collimation of lighting module 2
It is irradiated from the lower end of test sample 5, realizes and the fluorescence of 5 transmission-type of test sample is excited, test sample 5 is irradiated with a laser excitation
The fluorescent base of generation originally scatters upward, and the light-emitting window of lighting module is provided with one piece of optical filter.
Microcobjective 3 is the microcobjective of both sides' finite conjugate, and test sample 5 and detector 4 are located at microcobjective 3
At the focal position at both ends.One piece of filter is provided in microcobjective 3, for filtering the laser of the transmitting of light source 1, to avoid
Interference of the laser to fluorescence imaging.Microcobjective 3 collects the fluorescence that test sample 5 excites, and is transmitted through on detector 4 and is imaged.
The sequencing principle of the gene sequencing Optical devices of the present embodiment are as follows: light source 1 emits laser, lighting module vertically upward
2 laser is collimated after irradiation be located at the test sample 5 of upper end, test sample 5 generated fluorescence, microcobjective after laser excitation
3 collect the fluorescence that the excitation of test sample 5 generates, and will be imaged on Fluoroscopic to detector 4, realize in test sample 5
Base imaging sequencing.
The gene sequencing Optical devices of the present embodiment carry out test sample 5 since light source 1 is located at 5 lower section of test sample
Transmission-type illumination excitation, thus only need that the lighting module 2 for being used for collimation laser is arranged in the optical path of light source 1,
And the microcobjective 3 and detector 4 for collecting fluorescence imaging are successively set on 2 top of lighting module, i.e., in test sample 5
Side does not need other light such as lens barrel module, spectral module so that this gene sequencing Optical devices are located on a vertical straight line
Module is learned, therefore this gene sequencing structure of optical means is compact, simple, reduces complexity, can be realized miniaturization, and entire
Optical devices are located in a straight line, and adjustment requires to significantly reduce.
Use above specific case is illustrated the present invention, is merely used to help understand the present invention, not to limit
The system present invention.For those skilled in the art, according to the thought of the present invention, can also make several simple
It deduces, deform or replaces.
Claims (7)
1. a kind of gene sequencing Optical devices characterized by comprising
Light source, transmitting terminal are arranged upward, for emitting laser vertically upward；
Lighting module is mounted in the optical path of the light source transmitting laser, for will irradiate the survey being located above after laser alignment
Test agent；
Microcobjective is mounted on the surface of the lighting module, is irradiated with a laser what excitation generated for collecting test sample
Fluorescence；
Detector is mounted on the focal position of the microcobjective transmission fluorescence, for obtaining the glimmering of the microcobjective transmission
Light is simultaneously imaged.
2. gene sequencing Optical devices as described in claim 1, which is characterized in that the microcobjective is both sides' finite conjugate
Microcobjective.
3. gene sequencing Optical devices as claimed in claim 2, which is characterized in that be equipped in the microcobjective for ending
The filter of laser.
4. gene sequencing Optical devices as described in claim 1, which is characterized in that the lighting module is collimating mirror.
5. gene sequencing Optical devices as claimed in claim 4, which is characterized in that the light-emitting window of the lighting module is provided with
Filter.
6. gene sequencing Optical devices as described in claim 1, which is characterized in that the light source is a kind of or more for emitting
The laser generator of kind wavelength laser.
7. such as gene sequencing Optical devices as claimed in any one of claims 1 to 6, which is characterized in that further include being surveyed for placing
The transparent platform of test agent, the transparent platform is between the lighting module and microcobjective.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711365301.4A CN109932316A (en) | 2017-12-18 | 2017-12-18 | Gene sequencing Optical devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711365301.4A CN109932316A (en) | 2017-12-18 | 2017-12-18 | Gene sequencing Optical devices |
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| Publication Number | Publication Date |
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| CN109932316A true CN109932316A (en) | 2019-06-25 |
Family
ID=66982555
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201711365301.4A Pending CN109932316A (en) | 2017-12-18 | 2017-12-18 | Gene sequencing Optical devices |
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Cited By (2)
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| CN113031176A (en) * | 2019-12-24 | 2021-06-25 | 长春长光华大智造测序设备有限公司 | Optical fiber adjusting mechanism |
| CN116718578A (en) * | 2023-08-11 | 2023-09-08 | 深圳赛陆医疗科技有限公司 | Super-resolution microscopic imaging system and imaging method |
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| CB02 | Change of applicant information |
Address after: 130033 No. 1 Building, Incubation Base, 77 Yingkou Road, Changchun Economic Development Zone, Jilin Province Applicant after: Changchun Changguang Huada Zhizao sequencing Equipment Co.,Ltd. Address before: 130033 Building 1, hatchery, 77 Yingkou Road, Changchun Economic Zone, Jilin Applicant before: CHANGGUANG HUADA GENE SEQUENCING EQUIPMENT (CHANGCHUN) Co.,Ltd. |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190625 |
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| RJ01 | Rejection of invention patent application after publication |