CN109406478A - Based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and method - Google Patents
Based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and method Download PDFInfo
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- CN109406478A CN109406478A CN201811643165.5A CN201811643165A CN109406478A CN 109406478 A CN109406478 A CN 109406478A CN 201811643165 A CN201811643165 A CN 201811643165A CN 109406478 A CN109406478 A CN 109406478A
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- 239000007788 liquid Substances 0.000 title claims abstract description 69
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000008569 process Effects 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 10
- 230000003287 optical effect Effects 0.000 claims description 76
- KPHWPUGNDIVLNH-UHFFFAOYSA-M diclofenac sodium Chemical compound [Na+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KPHWPUGNDIVLNH-UHFFFAOYSA-M 0.000 claims description 22
- 239000012530 fluid Substances 0.000 claims description 17
- 230000008859 change Effects 0.000 claims description 14
- 229920006254 polymer film Polymers 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 9
- 238000003384 imaging method Methods 0.000 claims description 9
- 230000007246 mechanism Effects 0.000 claims description 9
- 238000001917 fluorescence detection Methods 0.000 claims description 6
- 230000010365 information processing Effects 0.000 claims description 4
- 238000009738 saturating Methods 0.000 claims description 3
- 238000004611 spectroscopical analysis Methods 0.000 claims description 3
- 238000004148 unit process Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 6
- 238000005259 measurement Methods 0.000 abstract description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 4
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
Abstract
The invention proposes one kind to be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device and its method, the detection device includes the light source emitter, collimation lens and narrow band filter slice being sequentially placed, the light source of light source emitter transmitting successively after collimated lens and narrow band filter slice, generates incident light source;The data information output end of CCD is connected with the ccd data information input terminal that information process unit is handled, and the adjusting information output of information process unit processing is connected with the adjusting information input terminal of liquid lens.This invention ensures that the repeatability of testing result, improves measurement accuracy and control flexibility ratio.
Description
Technical field
The present invention relates to a kind of technical field of spectral detection, are based on liquid lens automatic focusing laser more particularly to one kind
Induced fluorescence spectrum detection device and method.
Background technique
Laser induced fluorescence (LaserInducedFluorescence, LIF) spectral detection is most sensitive detection instantly
One of technology, concentration detection limit reach 10-9mol/L~10-13mol/L, and the fluorescent reagent high to certain fluorescence efficiencies is even
It can achieve Single Molecule Detection.
Detection sensitivity and signal-to-noise ratio, detection with higher can be effectively improved by being wherein total to focus type LIFD detection device
Sensitivity.Current total focus type LIFD detection method is usually to pass through manually to adjust focusing, and detection repeatability is by artificial
Factor is affected.Either by the way that by mobile whole optical imaging system, the imaging object plane for changing optical system is realized
Focal position focusing.Although adjustment process can be used automatically to replace extending using flexible to a certain extent manually,
It is to complete the adjustment of imaging object plane by mechanical displacement means to realize automatic focusing, controls flexibility ratio and measurement accuracy is still limited.
The unstable of mechanical structure movement simultaneously can bring certain error and noise, influence result of focusing.Using optotune as representative
The eyeglass of adjustable focus optical system be made of optical fluid and thin polymer film, the working principle and human lens of the eyeglass
It is similar, own form can be changed under the driving of different electric currents, to change the focal length of eyeglass.
Liquid is adjustable focus optical system has following advantage: several lis of the former eyeglass radius compared to conventional optical systems
Meter Bian Hua can be generated and the mobile several centimetres of equivalent optical effects of the lens set of the latter;Due to not being related to mirror in focussing process
The translation of piece, the former structure can completely enclose, and avoid the impurity such as dust intrusion device internal influence working performance and use
Service life;The former weight is far below conventional optical systems, and structure is compact more than conventional optical systems;The former response time is reachable
Millisecond rank, and repetitive positioning accuracy is high, and accumulated error is not present.
Summary of the invention
The present invention is directed at least solve the technical problems existing in the prior art, especially innovatively propose a kind of based on liquid
State lens automatic focusing laser-induced fluorescence spectroscopy detection device and method.
In order to realize above-mentioned purpose of the invention, the present invention provides one kind to be based on liquid lens automatic focusing induced with laser
Fluorescence spectrum detecting device, including light source generate optical path, fluorescent exciting road and imaging optical path;
It includes the light source emitter, collimation lens and narrow band filter slice being sequentially placed that light source, which generates optical path, the light source hair
The light source of emitter transmitting successively after collimated lens and narrow band filter slice, generates incident light source;
Fluorescent exciting road includes the liquid lens, microcobjective and article carrying platform set gradually, in the article carrying platform
On be placed with measuring samples, light source generates light source that optical path generates successively after liquid lens and microcobjective, focuses on to be checked
On sample, the measuring samples on focal plane are irradiated by focused light source, inspire fluorescence, then successively pass through microcobjective and liquid
After lens, outgoing fluorescence is generated;
Imaging optical path includes setting gradually two to dichronic mirror, band pass filter, the first condenser lens and CCD, and fluorescence swashs
The fluorescence that luminous road generates is acquired successively through two to after dichronic mirror, band pass filter and the first condenser lens by CCD;Two to points
Look mirror separates laser and fluorescence.
The data information output end of CCD is connected with the ccd data information input terminal that information process unit is handled, information processing
The adjusting information output of cell processing is connected with the adjusting information input terminal of liquid lens.
It in the preferred embodiment of the present invention, further include fluorescence detection optical path, the fluorescence detection optical path includes
Fluorescence beam splitter, the second condenser lens and the aperture set gradually, the fluorescence that fluorescent exciting road generates is successively through fluorescence
After beam splitter, the second condenser lens and aperture, by spectrometer collection;The data information output end of spectrometer and information processing
The spectroscopic data information input terminal of cell processing is connected.For deviateing the position of ideal focal plane, most of light can be by aperture
Diaphragm stops, and cannot be introduced into system, drops low environmental impact, enhancing interference.
In the preferred embodiment of the present invention, two are arranged to dichronic mirror in narrow band filter slice and liquid lens optical path
Between, two to dichronic mirror and optical axis straight line be in α, 0 < α < pi/2.
In the preferred embodiment of the present invention, two to dichronic mirror and optical axis straight line be in 45 °.
In the preferred embodiment of the present invention, the optical axis of the first condenser lens and the optical axis of microcobjective it is vertical or
In parallel.
In the preferred embodiment of the present invention, fluorescence beam splitter is arranged in band pass filter and the second condenser lens
Between optical path, the optical axis of fluorescence beam splitter and the first condenser lens is in β, 0 < β < pi/2.
In the preferred embodiment of the present invention, the optical axis of fluorescence beam splitter and the first condenser lens is in 45 °.
In the preferred embodiment of the present invention, it is 405 ± 1nm that the light source of the light source emitter transmitting, which is wavelength,
Ultraviolet laser;
Or/and described two can be reflected greater than to dichronic mirror or equal to 410nm wavelength light wave, and can transmit and be less than
The light wave of 410nm wavelength;
Or/and the incidence angle of the narrow band filter slice is γ, 0≤γ < pi/2;Narrow band filter slice allows the light wave wave penetrated
A length of 400~410nm.Only laser is allowed to pass through the interference of other veiling glares of elimination.
Or/and the incidence angle of the band pass filter is ε, 0≤ε < pi/2;Band pass filter allows the optical wavelength penetrated
For 421.5~900nm.
The automatic focusing laser-induced fluorescence spectroscopy detection method based on liquid lens that the present invention also provides a kind of, including
Following steps:
S31, record start position are Ai, Image Definition value is F (i) at this time, institute after mobile focusing step-length L
Place position is Ai+1, Image Definition is F (i+1);
S32, ifThen illustrate the accurate focal length of this detection range farther out, search pair
Focus effect influences less, then to keep the direction of search constant, while increasing focusing step-length is w1* L, the w1For just greater than 1
Number;Wherein, α is preset threshold, and max () is to take wherein the larger value, and i=i+1 executes step S32;
IfAnd F (i+1)-F (i) > ψ1, the ψ1For the first preset threshold and it is
Positive number;Then show that last focusing step-length is larger to focusing influential effect, then keeps focused search direction constant, reduce simultaneously
Focusing step-length is L/w2, the w2For the positive number greater than 1;I=i+1 executes step S32;
IfAnd F (i+1)-F (i) > ψ2, the ψ2For the second preset threshold and
For less than ψ1Positive number;Then illustrate that focus point has already appeared, change the direction of search, focusing step-length becomes L/w3, the w3For greater than
1 positive number;Execute step S33;
If direction of search change reaches w4It is secondary, the w4For the positive integer greater than 2;Execute step S33;
S33, Searching point changes the direction of search near focal point at this time, with minimum focusing stepping LminStart to search for,
The LminFor less than L/w3Positive number, until meeting F (i+1) < F (i), that is, until there is inflection point, at this time F (i) be inflection point, i.e.,
Focal position.
The present invention also provides one kind to be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, and liquid is saturating
Mirror includes fluid chamber and the elastic polymer film that is covered on it, and the elastic polymer film is used to limiting fills in fluid chamber
Optical liquid simultaneously generates required face shape under fluid chamber and extraneous pressure difference;It will when optical liquid is injected into fluid chamber
A normal pressure is generated, elastic polymer film is under pressure difference effect for outwardly convex, forms convex lens;When optical liquid is from liquid
When body cavity is extracted, negative pressure will be generated and form concavees lens;
It further include electromagnet actuation mechanism, the electromagnet actuation mechanism is to the pressure on fluid chamber periphery by flowing through the electric current of circuit
Directly by the control of size of current, liquid lens change under different electric current drivings for control, i.e. the focal length size of liquid lens
Become own form, to change the focal length of eyeglass.
In conclusion by adopting the above-described technical solution, the present invention realize can automatic focusing laser induced fluorescence
Detection device, it can according to the position of the feedback information adjust automatically focus point of image, in real time by laser be accurately positioned and
It focuses in test sample, the out-of-flatness for eliminating test sample causes laser that cannot focus on detection position, improves laser
Launching efficiency and fluorescence collection efficiency;Meanwhile sample by detection expand to face detect when, real-time tune can be rapidly performed by
Coke is used for quickly detecting the difference of sample;In addition, eliminate human error and mechanical structure it is mobile caused by it is unstable,
The repeatability that ensure that testing result improves measurement accuracy and control flexibility ratio.
Additional aspect and advantage of the invention will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Detailed description of the invention
Above-mentioned and/or additional aspect of the invention and advantage will become from the description of the embodiment in conjunction with the following figures
Obviously and it is readily appreciated that, in which:
Fig. 1 is light path schematic diagram of the present invention.
Fig. 2 is schematic process flow diagram of the present invention.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, and for explaining only the invention, and is not considered as limiting the invention.
The invention discloses one kind to be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, including light source
Generate optical path, fluorescent exciting road and imaging optical path.
It includes the light source emitter 1, collimation lens 2 and narrow band filter slice 3 being sequentially placed, the light source that light source, which generates optical path,
The light source that transmitter 1 emits successively after collimated lens 2 and narrow band filter slice 3, generates incident light source.In the present embodiment,
Light source emitter 1 is laser emitter, and the light source of transmitting is the ultraviolet laser that wavelength is 405 ± 1nm.Narrow band filter slice 3 enters
Firing angle is γ, 0≤γ < pi/2;Preferably, the incidence angle of the narrow band filter slice 3 is 0 °, and narrow band filter slice 3 allows to penetrate
Optical wavelength be 400~410nm.
Fluorescent exciting road includes the liquid lens 5, microcobjective 6 and article carrying platform 8 set gradually, flat in the loading
Measuring samples 7 are placed on platform 8, the light source that light source generation optical path generates successively after liquid lens 5 and microcobjective 6, focuses
Onto measuring samples 7, the measuring samples 7 on focal plane are irradiated by focused light source, inspire fluorescence, then successively pass through micro- object
After mirror 6 and liquid lens 5, outgoing fluorescence is generated.
Imaging optical path includes setting gradually two to dichronic mirror 4, band pass filter 9, the first condenser lens 11 and CCD15,
The fluorescence that fluorescent exciting road generates is successively through two to after dichronic mirror 4, band pass filter 9 and the first condenser lens 11, by CCD15
Acquisition.In the present embodiment, two can be reflected greater than to dichronic mirror 4 or equal to 410nm wavelength light wave, and can transmit and be less than
The light wave of 410nm wavelength.The incidence angle of the band pass filter 9 is ε, 0≤ε < pi/2;Preferably, the band pass filter 9
Incidence angle is 0 °, and the optical wavelength that band pass filter 9 allows to penetrate is 421.5~900nm.In addition, two to dichronic mirror 2
Be arranged between 5 optical path of narrow band filter slice 3 and liquid lens, two to dichronic mirror 2 and optical axis straight line be in α, 0 < α < pi/2;Preferably, two
It is in 45 ° to dichronic mirror 2 and optical axis straight line.
The data information output end of CCD15 is connected with the ccd data information input terminal of information process unit processing 16, information
The adjusting information output of processing unit processes 16 is connected with the adjusting information input terminal of liquid lens 5.In the present embodiment,
Information process unit processing 16 is computer.
It in the preferred embodiment of the present invention, further include fluorescence detection optical path, the fluorescence detection optical path includes
Fluorescence beam splitter 10, the second condenser lens 14 and the aperture 12 set gradually, the fluorescence that fluorescent exciting road generates is successively
After fluorescence beam splitter 10, the second condenser lens 14 and aperture 12, acquired by spectrometer 13;The data information of spectrometer 13
Output end is connected with the spectroscopic data information input terminal of information process unit processing 16.In the present embodiment, fluorescence beam splitter
10 are arranged between 14 optical path of band pass filter 9 and the second condenser lens, the optical axis of fluorescence beam splitter 10 and the first condenser lens 11
In β, 0 < β < pi/2.Preferably, the optical axis of fluorescence beam splitter 10 and the first condenser lens 11 is in 45 °.
In the preferred embodiment of the present invention, the optical axis of the optical axis of the first condenser lens 11 and microcobjective 6 hangs down
It is straight or parallel.When the optical axis of the first condenser lens 11 is vertical with the optical axis of microcobjective 6, as shown in Figure 1, including laser light source
1, collimation lens 2, narrow band filter slice 3, two to dichronic mirror 4, liquid lens 5, microcobjective 6, measuring samples 7, article carrying platform 8,
Band pass filter 9, fluorescence beam splitter 10, the first condenser lens 11, aperture 12, spectrometer 13, the second condenser lens 14,
CCD15, computer, are sequentially placed collimation lens 2, narrow band filter slice 3 in the 1 laser emitting optical path of laser, and two to color separation
Mirror 4, liquid lens 5, microcobjective 6, measuring samples 7, article carrying platform 8.The collimation lens 2, liquid lens 5, microcobjective 6
Optical axis keep a straight line, two to dichronic mirror 4 should with above-mentioned optical axis at 450 place.Described two to dichronic mirror 2 reflected light optical path
On, it is sequentially placed band pass filter 9, fluorescence beam splitter 10, the first condenser lens 11, aperture 12, spectrometer 13, described
The optical axis and optical axis of one condenser lens 11 keep vertical.The optical axis of the fluorescence beam splitter 10 and the first condenser lens 11 is at 45 °
It places, in the reflected light optical path of the fluorescence beam splitter 10, is sequentially placed the second condenser lens 14, CCD15, described second focuses
The optical axis of lens 14 and 11 optical axis of the first lens are vertical, and the computer is connected with liquid lens 5, CCD15 and spectrometer 13.
The working principle of the embodiment of the present invention is as follows:
1 launch wavelength of light source emitter is the ultraviolet laser of 405nm, and ultraviolet laser first passes through collimation lens 2, by standard
The straight collimation of lens 2 is collimated light beam, is then passed through narrow band filter 3, filters stray light by narrow band filter 3, then through two to points
Look mirror 4, then passes sequentially through liquid lens 5, microcobjective 6 focuses on measuring samples 7, and the measuring samples 7 on focal plane are logical
Over-focusing ultraviolet laser irradiation inspires fluorescence, and the fluorescence of the excitation direction opposite with ultraviolet laser incident direction is projected,
It passes sequentially through microcobjective 6, after liquid lens 5 are collected, is incident on two to being reflected after dichronic mirror 4, the fluorescence after reflection is logical
Band pass filter 9 is crossed, stray light is filtered, then by fluorescence beam splitter 10, fluorescence is divided into two beams, the first beam by fluorescence beam splitter 10
Fluorescence passes through the first condenser lens 11, aperture 12, and aperture 12 filters the light on non-focal plane, glimmering on focal plane
Light enters spectroanalysis instrument 13, by photoelectric information conversion, the fluorescence spectrum of a full spectral wavelength is formed, by fluorescence spectrum
It is transferred to the spectrum analysis terminal of installation on computers and carries out information processing, obtain the spectral information of test sample;Second beam
Fluorescence passes through the second condenser lens 14, and fluorescence is converged on CCD15 imaging sensor, the image of test sample is obtained, will be schemed
As being transferred to the image processing software of installation on computers, image definition analysis is executed, auto-focusing algorithm is then used
It is calculated, parameter of focusing is transferred to the drive control module 51 of liquid lens 5, drive control module by output focusing parameter
Parameter Switch will be focused into current signal, be transferred to the control mechanism 52 of liquid eyeglass, control mechanism 52 controls liquid eyeglass mould
Block 53 changes shape, realizes focusing.
The automatic focusing laser-induced fluorescence spectroscopy detection method based on liquid lens that the invention also discloses a kind of is such as schemed
Shown in 2, comprising the following steps:
S1, information process unit control the CCD and acquire image information;
S2, information process unit execute image definition evaluation;Judge image definition whether standard, if image definition
Standard thens follow the steps S4;If image definition is nonstandard, S3 is thened follow the steps;In the present embodiment, image definition is commented
Valence is the transformation to gray value of image, generally chooses gray value in a region of same image or several regions of same image
Sum perhaps quadratic sum or a few powers and, be also possible to based on gray value gradient, image entropy, based on frequency domain Fourier transformation
The either value of wavelet transformation.Preferably, the present invention is the shade of gray function using Sobel as image definition evaluation.
S3 calculates the parameter for needing to focus according to auto-focusing algorithm, is input to the drive module of lens, returns to step
Rapid S1;
Wherein, the specific steps of step S3 are as follows: quick coarse adjustment is carried out using big step-length in defocus remote position, and in coke
Point nearby uses the accurate fine tuning of small step-length instead.
S31, record start position are Ai, Image Definition value is F (i) at this time, institute after mobile focusing step-length L
Place position is Ai+1, Image Definition is F (i+1);
S32, ifThen illustrate the accurate focal length of this detection range farther out, search pair
Focus effect influences less, then to keep the direction of search constant, while increasing focusing step-length is w1* L, the w1For just greater than 1
Number;Wherein, α is preset threshold, and max () is to take wherein the larger value, and i=i+1 executes step S32;Preferably, w1=2, generally
α takes the intermediate value or mean value or other weight values calculated according to specific condition of pixel grey scale, and taking absolute value is to support
Disappear and generates direction erroneous judgement since noise causes evaluation function fine jitter.
IfAnd F (i+1)-F (i) > ψ1, the ψ1For the first preset threshold and it is
Positive number;Then show that last focusing step-length is larger to focusing influential effect, then keeps focused search direction constant, reduce simultaneously
Focusing step-length is L/w2, the w2For the positive number greater than 1;I=i+1 executes step S32;Preferably, w2=2.
IfAnd F (i+1)-F (i) > ψ2, the ψ2For the second preset threshold and it is
Less than ψ1Positive number;Then illustrate that focus point has already appeared, change the direction of search, focusing step-length becomes L/w3, the w3For greater than 1
Positive number;Execute step S33;Wherein, F (i+1)-F (i) > ψ1Increase obviously for F (i+1) than F (i);F (i+1)-F (i) > ψ2
It is obviously reduced for F (i+1) than F (i).Preferably, w3=2.
If direction of search change reaches w4It is secondary, the w4For the positive integer greater than 2;Execute step S33;Preferably, w4=3.
S33, Searching point changes the direction of search near focal point at this time, with minimum focusing stepping LminStart to search for,
The LminFor less than L/w3Positive number, until meeting F (i+1) < F (i), that is, until there is inflection point, at this time F (i) be inflection point, i.e.,
Focal position.
S4 terminates.
The present invention also provides one kind to be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, and liquid is saturating
Mirror includes fluid chamber and the elastic polymer film that is covered on it, and the elastic polymer film is used to limiting fills in fluid chamber
Optical liquid simultaneously generates required face shape under fluid chamber and extraneous pressure difference;It will when optical liquid is injected into fluid chamber
A normal pressure is generated, elastic polymer film is under pressure difference effect for outwardly convex, forms convex lens;When optical liquid is from liquid
When body cavity is extracted, negative pressure will be generated and form concavees lens;I.e. liquid lens are a Curvature varying lens,
It further include electromagnet actuation mechanism, the electromagnet actuation mechanism is to the pressure on fluid chamber periphery by flowing through the electric current of circuit
Directly by the control of size of current, liquid lens 5 change under different electric current drivings for control, i.e. the focal length size of liquid lens
Become own form, to change the focal length of eyeglass.Magnetic actuation mechanism will need the parameter focused to be converted to current signal, be applied to
Liquid lens.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that: not
A variety of change, modification, replacement and modification can be carried out to these embodiments in the case where being detached from the principle of the present invention and objective, this
The range of invention is defined by the claims and their equivalents.
Claims (10)
1. one kind is based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, which is characterized in that raw including light source
At optical path, fluorescent exciting road and imaging optical path;
It includes the light source emitter (1), collimation lens (2) and narrow band filter slice (3) being sequentially placed, the light that light source, which generates optical path,
The light source of source transmitter (1) transmitting is successively after collimated lens (2) and narrow band filter slice (3), generation incident light source;
Fluorescent exciting road includes the liquid lens (5), microcobjective (6) and article carrying platform (8) set gradually, in the loading
It is placed on platform (8) measuring samples (7), light source generates the light source of optical path generation successively through liquid lens (5) and microcobjective
(6) it after, focusing on measuring samples (7), the measuring samples (7) on focal plane are irradiated by focused light source, fluorescence is inspired, then
Successively after microcobjective (6) and liquid lens (5), outgoing fluorescence is generated;
Imaging optical path includes setting gradually two to dichronic mirror (4), band pass filter (9), the first condenser lens (11) and CCD
(15), the fluorescence that fluorescent exciting road generates is successively through two to dichronic mirror (4), band pass filter (9) and the first condenser lens
(11) it after, is acquired by CCD (15);
The data information output end of CCD (15) is connected with the ccd data information input terminal of information process unit processing (16), information
The adjusting information output of processing unit processes (16) is connected with the adjusting information input terminal of liquid lens (5).
2. according to claim 1 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, further includes fluorescence detection optical path, the fluorescence detection optical path includes the fluorescence beam splitter (10) set gradually, the second focusing
Lens (14) and aperture (12), the fluorescence that fluorescent exciting road generates successively focus through fluorescence beam splitter (10), second saturating
After mirror (14) and aperture (12), acquired by spectrometer (13);The data information output end of spectrometer (13) and information processing
The spectroscopic data information input terminal of cell processing (16) is connected.
3. according to claim 2 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, two are arranged between narrow band filter slice (3) and liquid lens (5) optical path to dichronic mirror (2), and two to dichronic mirror (2) and optical axis
Straight line is in α, 0 < α < pi/2.
4. according to claim 3 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
Be, two to dichronic mirror (2) and optical axis straight line be in 45 °.
5. according to claim 2 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, the optical axis of the optical axis and microcobjective (6) of the first condenser lens (11) is perpendicular or parallel.
6. according to claim 5 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, fluorescence beam splitter (10) is arranged between band pass filter (9) and the second condenser lens (14) optical path, fluorescence beam splitter (10)
Optical axis with the first condenser lens (11) is in β, 0 < β < pi/2.
7. according to claim 6 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, the optical axis of fluorescence beam splitter (10) and the first condenser lens (11) is in 45 °.
8. according to claim 1 be based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, feature
It is, the light source of light source emitter (1) transmitting is the ultraviolet laser that wavelength is 405 ± 1nm;
Or/and described two can be reflected greater than to dichronic mirror (4) or equal to 410nm wavelength light wave, and can transmit and be less than 410nm
The light wave of wavelength;
Or/and the incidence angle of the narrow band filter slice (3) is γ, 0≤γ < pi/2;Narrow band filter slice (3) allows the light wave penetrated
Wavelength is 400~410nm;
Or/and the incidence angle of the band pass filter (9) is ε, 0≤ε < pi/2;Band pass filter (9) allows the light wave wave penetrated
A length of 421.5~900nm.
9. one kind is based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection method, which is characterized in that including following step
It is rapid:
S31, record start position are Ai, Image Definition value is F (i) at this time, locating position after mobile focusing step-length L
It is set to Ai+1, Image Definition is F (i+1);
S32, ifThen illustrate the accurate focal length of this detection range farther out, searches for focusing
Influential effect is little, then keeps the direction of search constant, while increasing focusing step-length is w1* L, the w1For the positive number greater than 1;Its
In, α is preset threshold, and max () is to take wherein the larger value, and i=i+1 executes step S32;
IfAnd F (i+1)-F (i) > ψ1, the ψ1For the first preset threshold and it is positive
Number;Then show that last focusing step-length is larger to focusing influential effect, then keeps focused search direction constant, while reducing tune
Burnt step-length is L/w2, the w2For the positive number greater than 1;I=i+1 executes step S32;
IfAnd F (i+1)-F (i) > ψ2, the ψ2For the second preset threshold and for less than
ψ1Positive number;Then illustrate that focus point has already appeared, change the direction of search, focusing step-length becomes L/w3, the w3For just greater than 1
Number;Execute step S33;
If direction of search change reaches w4It is secondary, the w4For the positive integer greater than 2;Execute step S33;
S33, Searching point changes the direction of search near focal point at this time, with minimum focusing stepping LminStart to search for, it is described
LminFor less than L/w3Positive number, until meeting F (i+1) < F (i), that is, until there is inflection point, F (i) is inflection point, i.e. focus at this time
Position.
10. one kind is based on liquid lens automatic focusing laser-induced fluorescence spectroscopy detection device, which is characterized in that liquid lens packet
The elastic polymer film for including fluid chamber and being covered on it, the elastic polymer film are used to limit the optics filled in fluid chamber
Liquid simultaneously generates required face shape under fluid chamber and extraneous pressure difference;It will be generated when optical liquid is injected into fluid chamber
One normal pressure, elastic polymer film are under pressure difference effect for outwardly convex, form convex lens;When optical liquid is from fluid chamber
When being extracted, negative pressure will be generated and form concavees lens;
It further include electromagnet actuation mechanism, the electromagnet actuation mechanism is to the pressure on fluid chamber periphery by flowing through the electric current control of circuit
Directly by the control of size of current, liquid lens (5) change under different electric current drivings for system, i.e. the focal length size of liquid lens
Become own form, to change the focal length of eyeglass.
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