CN105582573A - 一种纳微米多尺度壳聚糖三维支架及其制备方法 - Google Patents
一种纳微米多尺度壳聚糖三维支架及其制备方法 Download PDFInfo
- Publication number
- CN105582573A CN105582573A CN201610106724.3A CN201610106724A CN105582573A CN 105582573 A CN105582573 A CN 105582573A CN 201610106724 A CN201610106724 A CN 201610106724A CN 105582573 A CN105582573 A CN 105582573A
- Authority
- CN
- China
- Prior art keywords
- micron
- chitosan
- preparation
- nano
- multiple dimensioned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000835 fiber Substances 0.000 claims abstract description 31
- 238000004108 freeze drying Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 5
- 229920002101 Chitin Polymers 0.000 claims description 16
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 239000004088 foaming agent Substances 0.000 claims description 10
- 238000009987 spinning Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- APSBXTVYXVQYAB-UHFFFAOYSA-M sodium docusate Chemical compound [Na+].CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC APSBXTVYXVQYAB-UHFFFAOYSA-M 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 238000006073 displacement reaction Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 11
- 150000003839 salts Chemical class 0.000 abstract description 7
- 239000011148 porous material Substances 0.000 abstract description 4
- 238000000465 moulding Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000012528 membrane Substances 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- 238000007605 air drying Methods 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000010008 shearing Methods 0.000 abstract 1
- 238000013508 migration Methods 0.000 description 5
- 230000005012 migration Effects 0.000 description 5
- 239000005030 aluminium foil Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 239000002121 nanofiber Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- SQDAZGGFXASXDW-UHFFFAOYSA-N 5-bromo-2-(trifluoromethoxy)pyridine Chemical compound FC(F)(F)OC1=CC=C(Br)C=N1 SQDAZGGFXASXDW-UHFFFAOYSA-N 0.000 description 1
- 229920001287 Chondroitin sulfate Polymers 0.000 description 1
- 241000264877 Hippospongia communis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003519 biomedical and dental material Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229940059329 chondroitin sulfate Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000002062 molecular scaffold Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/26—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2201/00—Foams characterised by the foaming process
- C08J2201/04—Foams characterised by the foaming process characterised by the elimination of a liquid or solid component, e.g. precipitation, leaching out, evaporation
- C08J2201/044—Elimination of an inorganic solid phase
- C08J2201/0444—Salts
- C08J2201/0446—Elimination of NaCl only
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Textile Engineering (AREA)
- Dermatology (AREA)
- Dispersion Chemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Materials For Medical Uses (AREA)
Abstract
本发明涉及一种纳微米多尺度壳聚糖三维支架及其制备方法，它采用在一定条件下分别静电纺丝纳米和微米壳聚糖纤维膜，经碱洗、水洗和风干后按一定配比将纳米和微米壳聚糖纤维膜在水中高速剪切成短纤维，分样后，加入微米尺度的盐颗粒作为致孔剂，快速在液氮中冷冻成型并冻干，冻干样品经水置换去除致孔剂，再经冷冻干燥得到纳微米多尺度壳聚糖三维支架。该制备方法工艺简单，支架在纤维直径和孔径同时具有微纳米尺度，赋予支架良好的生物学性能和力学性能。
Description
技术领域
本发明涉及一种纳微米多尺度壳聚糖三维支架及其制备方法，属于生物医用材料技术领域。
背景技术
壳聚糖作为一种阳离子碱性多糖，结构类似于人体内的硫酸软骨素和透明质酸等多糖类物质，在体内可被降解吸收，并具有止痛、止血、抑菌、促进伤口愈合、减小疤痕等优异特性，目前在生物医用材料领域有重要的应用。SundararajanV等人采用相分离技术首次制备了壳聚糖支架，其结构为数十到数百微米厚度的片状蜂窝结构，具有较好的生物学性能（Biomaterials,1999;20:1133-1142）。胡巧玲等公开了三维有序多孔壳聚糖骨修复支架材料的制备方法（CN101366972），拓展了其应用。壳聚糖具有良好的生物相容性，研究证明了其可作为支架材料，但壳聚糖支架的微观结构也对其综合性能有较大的影响。研究表明纳米纤维支架有较大的比表面积，可提供细胞粘附和分化的更好条件，但单纯的纳米纤维力学强度较低，无法承受支架植入过程的力学载荷。相比而言，微米纤维支架具有较好的力学性能。另外，支架要求具有三维贯通的孔结构，便于营养物质的传递、代谢物质的交换以及细胞的迁移。研究表明，对于营养物质的传递和代谢物质的交换，纳米尺度的孔具有较高的效率，并可促进支架的降解吸收和组织再生，但该尺度的孔阻碍细胞在支架内部的渗透和迁移，微米尺度的孔则对细胞的渗透、迁移以及随之的分化增殖、新血管和神经的长入是比较有利的。微纳米共存的孔结构可以保证细胞生长过程中营养物质的传递、细胞的迁移以及新血管和神经生长所需的空间。
综上，在纤维直径和孔径同时具有微纳米多尺度的三维支架具有良好的生物相容性和力学性能。目前研究者多采用相分离的方法制备壳聚糖三维支架，但无法控制其纤维直径和孔径，或采用静电纺丝的方法获得二维的壳聚糖薄膜，但如何制备纤维直径和孔径同时具有微纳米多尺度的三维壳聚糖支架制备方法尚未见报导。
发明内容
本发明的主要设想是采用静电纺丝的方法分别制备纳米和微米壳聚糖薄膜，按一定配比将纳米和微米壳聚糖纤维膜混合，并将壳聚糖薄膜高速剪切成短纤维，分样后，加入微米尺度的致孔剂，经冷冻干燥成型，水置换去除致孔剂，再经冷冻干燥得到纳微米多尺度壳聚糖三维支架。
本发明中纳微米多尺度壳聚糖三维支架的制备方法具体如下所述。
(1)静电纺丝纳米壳聚糖纤维膜，配制壳聚糖5.0%的溶液，溶剂为三氟乙酸和二噁烷的混合物，体积比为70:30，纺丝电压为20kV。
(2)静电纺丝微米壳聚糖纤维膜，配制壳聚糖7.5%的溶液，溶剂为三氟乙酸和二噁烷的混合物，体积比为90:10，纺丝电压为15kV。
(3)将静电纺丝得到的纳米和微米壳聚糖纤维膜浸泡在0.5mol/L的氢氧化钠溶液中去除未挥发的溶剂，水洗风干后待用。
(4)分别称取定量的纳米和微米壳聚糖纤维膜，加入到定量的水中，在水中高速剪切成短纤维。
(5)分样至称量瓶中，加入筛分后具有一定粒度分布的盐作为致孔剂，快速在液氮中冷冻成型并冻干。
(6)冻干后的样品经水置换去除盐，再经冷冻干燥可获得纳微米多尺度壳聚糖三维支架。
本发明制得的纳微米多尺度壳聚糖三维支架外观为白色圆盘，其形状和高度可在分样中采用不同的容器和分样体积调节。制备方法中主要通过静电纺丝获得纳微米尺度的纤维，制备中也可方便地调整纺丝条件得到不同尺度的纳米和微米壳聚糖纤维，通过纳米纤维之间的孔形成纳米尺度的孔，通过微米尺度的盐致孔剂获得微米尺度的孔。该支架在纤维直径和孔径同时具有微纳米尺度，赋予支架良好的生物相容性和力学强度，便于营养物质的输送和细胞的迁移。该制备方法工艺简单，易于得到不同直径的壳聚糖纤维，并可通过盐致孔获得微米尺度的孔，其孔径容易控制。相比于单纯的静电纺丝法，该法还可容易地制备三维结构的支架。
具体实施方式
下面结合具体实施例，对本发明内容作进一步的说明，但本发明的实现方式并不局限于此。
实施例1：分别取7ml的三氟乙酸和3ml的二噁烷，混合均匀，称取0.5g壳聚糖，搅拌溶解，静置12小时。将壳聚糖溶液加入到注射器中，通以20kV的电压纺丝，收集在接地的铝箔上，可获得壳聚糖纤维直径在50-300nm的薄膜。分别取18ml的三氟乙酸和2ml的二噁烷，混合均匀，称取1.5g壳聚糖，搅拌溶解，静置12小时。将壳聚糖溶液加入到注射器中，通以15kV的电压纺丝，收集在接地的铝箔上，可获得壳聚糖纤维直径在1-10μm的薄膜。将静电纺丝得到的纳米和微米纤维膜浸泡在0.5mol/L的氢氧化钠溶液中去除未挥发的溶剂，水洗风干。分别称取0.2g和0.8g纳米和微米壳聚糖纤维膜，在100ml水中高速剪切成短纤维，分样至称量瓶中，加入筛分的氯化钠颗粒，其粒度分布在50-350μm之间，高度与分样的液面相同，快速在液氮中冷冻成型，在冻干机中冻干。冻干后的样品经水置换去除支架中的盐致孔剂，再经冷冻干燥可获得纳微米多尺度壳聚糖三维支架。
实施例2：分别取35ml的三氟乙酸和15ml的二噁烷，混合均匀，称取2.5g壳聚糖，搅拌溶解，静置12小时。将壳聚糖溶液加入到注射器中，通以20kV的电压纺丝，收集在接地的铝箔上，可获得壳聚糖纤维直径在50-300nm的薄膜。分别取9ml的三氟乙酸和1ml的二噁烷，混合均匀，称取0.75g壳聚糖，搅拌溶解，静置12小时。将壳聚糖溶液加入到注射器中，通以15kV的电压纺丝，收集在接地的铝箔上，可获得壳聚糖纤维直径在1-10μm的薄膜。将静电纺丝得到的纳米和微米纤维膜浸泡在0.5mol/L的氢氧化钠溶液中去除未挥发的溶剂，水洗风干。分别称取2.4g和0.6g纳米和微米壳聚糖纤维膜，在100ml水中高速剪切成短纤维，分样至称量瓶中，加入筛分的氯化钠颗粒，其粒度分布在50-350μm之间，高度与分样的液面相同，快速在液氮中冷冻成型，在冻干机中冻干。冻干后的样品经水置换去除支架中的盐致孔剂，再经冷冻干燥可获得纳微米多尺度壳聚糖三维支架。
Claims (6)
1.一种纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于在一定条件下分别静电纺丝纳米和微米壳聚糖纤维膜，在0.5mol/L的氢氧化钠溶液中去除未挥发的溶剂并水洗风干后，按一定配比将纳米和微米壳聚糖纤维膜在水中高速剪切成短纤维，分样后，加入具有一定粒度分布的微米氯化钠致孔剂，快速在液氮中冷冻成型并冻干，冻干样品经水置换去除致孔剂，水置换去除氯化钠致孔剂，再经冷冻干燥得到纳微米多尺度壳聚糖三维支架。
2.根据权利要求1所述的纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于所使用的壳聚糖分子量在20～40万之间。
3.根据权利要求1所述的纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于静电纺丝纳米纤维膜时，壳聚糖浓度为5.0%，溶剂为三氟乙酸和二噁烷的混合物，体积比为70:30，纺丝电压为20kV。
4.根据权利要求1所述的纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于静电纺丝微米壳聚糖纤维膜时，壳聚糖浓度为7.5%，溶剂为三氟乙酸和二噁烷的混合物，体积比为90:10，纺丝电压为15kV。
5.根据权利要求1所述的纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于纳米壳聚糖和微米壳聚糖的质量比在20:80到80:20之间，壳聚糖纳米微米纤维在水中的总浓度在1～3%之间。
6.根据权利要求1所述的纳微米多尺度壳聚糖三维支架及其制备方法，其特征在于使用的氯化钠致孔剂的粒度范围在50～350μm之间。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106724.3A CN105582573B (zh) | 2016-02-27 | 2016-02-27 | 一种纳微米多尺度壳聚糖三维支架及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610106724.3A CN105582573B (zh) | 2016-02-27 | 2016-02-27 | 一种纳微米多尺度壳聚糖三维支架及其制备方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105582573A true CN105582573A (zh) | 2016-05-18 |
| CN105582573B CN105582573B (zh) | 2018-10-26 |
Family
ID=55922719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610106724.3A Expired - Fee Related CN105582573B (zh) | 2016-02-27 | 2016-02-27 | 一种纳微米多尺度壳聚糖三维支架及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105582573B (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107185037A (zh) * | 2017-06-09 | 2017-09-22 | 青岛大学 | 一种壳聚糖微纳米纤维状三维多孔支架及其制备方法 |
| CN107376013A (zh) * | 2017-06-09 | 2017-11-24 | 青岛大学 | 一种多孔微纳米纤维三维壳聚糖支架及其制备方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103285424A (zh) * | 2013-05-27 | 2013-09-11 | 东华大学 | 一种三维纤维基气凝胶组织工程支架及其制备方法 |
| CN103691005A (zh) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | 一种微-纳纤维组织工程支架及其制备方法 |
| WO2014160019A1 (en) * | 2013-03-14 | 2014-10-02 | Lifenet Health | Aligned fiber and method of use thereof |
| CN104888278A (zh) * | 2015-05-20 | 2015-09-09 | 东华大学 | 一种纳/微米纤维三维多孔结构支架材料及其制备和应用 |
| CN105107022A (zh) * | 2015-09-21 | 2015-12-02 | 东华大学 | 一种在湿态下具有压缩弹性的纳米纤维多孔支架的制备方法 |
-
2016
- 2016-02-27 CN CN201610106724.3A patent/CN105582573B/zh not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014160019A1 (en) * | 2013-03-14 | 2014-10-02 | Lifenet Health | Aligned fiber and method of use thereof |
| CN103285424A (zh) * | 2013-05-27 | 2013-09-11 | 东华大学 | 一种三维纤维基气凝胶组织工程支架及其制备方法 |
| CN103691005A (zh) * | 2013-12-24 | 2014-04-02 | 华东交通大学 | 一种微-纳纤维组织工程支架及其制备方法 |
| CN104888278A (zh) * | 2015-05-20 | 2015-09-09 | 东华大学 | 一种纳/微米纤维三维多孔结构支架材料及其制备和应用 |
| CN105107022A (zh) * | 2015-09-21 | 2015-12-02 | 东华大学 | 一种在湿态下具有压缩弹性的纳米纤维多孔支架的制备方法 |
Non-Patent Citations (1)
| Title |
|---|
| 高庆东等: "基于组织工程研究的可降解支架材料选择策略", 《生物工程学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107185037A (zh) * | 2017-06-09 | 2017-09-22 | 青岛大学 | 一种壳聚糖微纳米纤维状三维多孔支架及其制备方法 |
| CN107376013A (zh) * | 2017-06-09 | 2017-11-24 | 青岛大学 | 一种多孔微纳米纤维三维壳聚糖支架及其制备方法 |
| CN107185037B (zh) * | 2017-06-09 | 2020-03-31 | 青岛大学 | 一种壳聚糖微纳米纤维状三维多孔支架及其制备方法 |
| CN107376013B (zh) * | 2017-06-09 | 2020-03-31 | 青岛大学 | 一种多孔微纳米纤维三维壳聚糖支架及其制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105582573B (zh) | 2018-10-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Han et al. | A review: Current status and emerging developments on natural polymer‐based electrospun fibers | |
| Gu et al. | Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials | |
| Zhao et al. | Construction of highly biocompatible hydroxyethyl cellulose/soy protein isolate composite sponges for tissue engineering | |
| Radhakrishnan et al. | PEG-penetrated chitosan–alginate co-polysaccharide-based partially and fully cross-linked hydrogels as ECM mimic for tissue engineering applications | |
| CN103993424A (zh) | 一种聚氨酯-角蛋白复合纳米纤维膜的制备方法 | |
| CN105641744B (zh) | 一种纳微米多尺度壳聚糖聚乳酸复合支架及其制备方法 | |
| CN104027846A (zh) | 一种非织造材料增强组织工程复合三维支架及其制备方法 | |
| EP1144592B1 (en) | Substrate for cell growth | |
| CN104004221A (zh) | 一种聚己内酯-角蛋白复合多孔支架的制备方法 | |
| Zhang et al. | Self-assembly of chitosan and cellulose chains into a 3D porous polysaccharide alloy films: Co-dissolving, structure and biological properties | |
| CN104005179A (zh) | 一种聚己内酯-角蛋白复合纳米纤维管的制备方法 | |
| CN107802888A (zh) | 一种促进软骨再生的纳米纤维支架的制备方法 | |
| CN105664246B (zh) | 一种纳微米多尺度组织工程复合三维支架及其制备方法 | |
| CN111188194B (zh) | 一种丝素蛋白/明胶纤维增强壳聚糖复合止血材料的制备方法 | |
| CN103993425A (zh) | 一种聚己内酯-角蛋白复合纳米纤维膜的制备方法 | |
| CN109364288B (zh) | 坑-孔复合微纳结构多聚糖微球在制备止血敷料的用途 | |
| CN105582573A (zh) | 一种纳微米多尺度壳聚糖三维支架及其制备方法 | |
| Zhang et al. | The root-like chitosan nanofiber porous scaffold cross-linked by genipin with type I collagen and its osteoblast compatibility | |
| CN105536075A (zh) | 一种纳微米纤维壳聚糖聚乳酸复合支架及其制备方法 | |
| CN104017230A (zh) | 一种聚氨酯-角蛋白复合膜的制备方法 | |
| CN109675089A (zh) | 促进皮肤创伤愈合的生物支架材料及其制备方法和用途 | |
| CN108553689B (zh) | 一种具有纳米纤维微结构的丝素多孔微球及其制备方法 | |
| CN109517225B (zh) | 坑-孔复合微纳结构多聚糖微球及制备方法 | |
| CN105727364A (zh) | 一种纳微米多尺度聚乳酸三维支架及其制备方法 | |
| CN105749340B (zh) | 一种纳微米纤维组织工程三维复合支架及其制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181026 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |