CN105582042A - Traditional Chinese medicine composition for treating pollen allergy - Google Patents
Traditional Chinese medicine composition for treating pollen allergy Download PDFInfo
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- CN105582042A CN105582042A CN201511010733.4A CN201511010733A CN105582042A CN 105582042 A CN105582042 A CN 105582042A CN 201511010733 A CN201511010733 A CN 201511010733A CN 105582042 A CN105582042 A CN 105582042A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/17—Gnetophyta, e.g. Ephedraceae (Mormon-tea family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
- A61K36/428—Trichosanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses traditional Chinese medicine composition for treating pollen allergy. The traditional Chinese medicine composition is prepared from extracts of ephedra herbs and mongolian snakegourd roots and can be prepared in any oral dosage form in pharmacy, such as tablets, capsules, granules, pills and syrup. Raw materials are simple, by comparison with other composition for treating pollen allergy, the cost is reduced, a specific traditional Chinese medicine extraction method is adopted, the extraction rate of effective ingredients of traditional Chinese medicines is increased, the treatment effect is improved, and the traditional Chinese medicine composition has few toxic and side effects.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition for the treatment of pollen hypersensitivity, belong to field of medicaments.
Background technology
At nature, pollen is a kind of main sensibiligen. When during the warmth of spring, all the flowers bloom, the most easily cause pollen hypersensitivityMostly be seed tree, as Chinese holly tree, castor-oil plant, summer cypress, oriental plane tree etc. These plant pollen amounts are large, volume is little, and in air, content is high,The date of rising at wind more easily propagates, so occur that pollen hypersensitivity person is more when spring outing.
After the oil containing in pollen and polysaccharide material are sucked by people, can be digested by the secretion of nasal cavity, discharge subsequentlyTen Multiple Antibodies. If the pollen of this antibody and invasion meets, and put aside in a large number, will cause allergic.
The composition for the treatment of pollen hypersensitivity few at present, and result for the treatment of is not remarkable yet.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine composition for the treatment of pollen hypersensitivity, by respectively from two kinds of Chinese medicinesExtract composition, curative effect is better, more stable than Western medicine, and has no side effect.
A Chinese medicine composition for the treatment of pollen hypersensitivity, comprises raw material: Chinese ephedra, root of Chinese trichosanthes.
Treat a Chinese medicine composition for pollen hypersensitivity, comprise the raw material of following mass fraction: Herba Ephedrae extract 4-8 part,Radix Trichosanthis extract 11-15 part, mixes.
The extracting method of described Herba Ephedrae extract is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 3-5 times of weight, 70-80 DEG C of heating and refluxing extraction 2 times, each40-60min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is1-1.5mL/min, and then with the O.5-1.5mol/L ammoniacal liquor of 2-4BV, 1.0-2.0mol/L ammoniacal liquor and 80% ethanol of 2-4BVThe 2.0-3.0mol/L ammoniacal liquor of mixed liquor, 2-4BV and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 1-1.5mL/Min, merges eluent;
(3) eluent reduced pressure concentration, obtains the medicinal extract that relative density is 1.05-1.10, is Herba Ephedrae extract.
Wherein, the described ion exchange resin column of step (2) be D113, D155, the series connection of HD-1 type ion exchange resin andBecome, the height of resin column and diameter are than being 4:1.
Wherein, the volume ratio of the filtrate of step (2), D113, D155, HD-1 type ion exchange resin is: 1:0.2-0.3:0.2-0.3:0.2-0.3。
Wherein, the volume ratio of 1.0-2.0mol/L ammoniacal liquor and 80% ethanol is 1:1-5,2.0-3.0mol/L ammoniacal liquor and 70%The volume ratio of ethanol is 1:1-5.
The extracting method of described Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixed of 3-5 times of weightClose solution, 60-70 DEG C of refluxing extraction 100-140min, filters to get filtrate, reduced pressure concentration, and obtaining relative density is 1.05-1.10'sMedicinal extract, is Radix Trichosanthis extract.
Wherein, described mixed solution is 95% ethanol, acetone, n-butanol, and its volume ratio is 1:0.5-0.9:0.4-0.6.
Treat a Chinese medicine composition for pollen hypersensitivity, after raw material mixes, make in any pharmacy saidPeroral dosage form.
Described peroral dosage form is: tablet, capsule, granule, pill, syrup.
Raw material of the present invention is simple, compared with the composition of other treatment pollen hypersensitivity, has reduced cost, has adopted spy simultaneouslyFixed Traditional Chinese medicine extraction method, has extracted the active ingredient for the treatment of pollen hypersensitivity, has improved result for the treatment of, and toxic and side effectLittle.
Specific embodiment:
Embodiment 1:
Treat a Chinese medicine composition for pollen hypersensitivity, comprise the raw material of following mass fraction: 4 parts of Herba Ephedrae extracts, root of Chinese trichosanthes11 parts of extracts, mix.
The extracting method of described Herba Ephedrae extract is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 3 times of weight, 70 DEG C of heating and refluxing extraction 2 times, each40min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is1mL/min, and then with the 1.0mol/L ammoniacal liquor of the O.5-1.5mol/L ammoniacal liquor of 2BV, 2BV and 80% alcohol mixeding liquid, 2BV2.0mol/L ammoniacal liquor and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 1mL/min, merge eluent;
(3) eluent reduced pressure concentration, obtains relative density and is 1.05 medicinal extract, is Herba Ephedrae extract.
Wherein, the described ion exchange resin column of step (2) be D113, D155, the series connection of HD-1 type ion exchange resin andBecome, the height of resin column and diameter are than being 4:1.
Wherein, the volume ratio of the filtrate of step (2), D113, D155, HD-1 type ion exchange resin is: 1:0.2:0.2:0.2。
Wherein, the volume ratio of 1.0mol/L ammoniacal liquor and 80% ethanol is 1:1, the volume of 2.0mol/L ammoniacal liquor and 70% ethanolThan being 1:1.
The extracting method of described Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixed of 3-5 times of weightClose solution, 60 DEG C of refluxing extraction 100min, filter to get filtrate, and reduced pressure concentration obtains relative density and be 1.05 medicinal extract, is skyPollen extract.
Wherein, described mixed solution is 95% ethanol, acetone, n-butanol, and its volume ratio is 1:0.5:0.4.
Embodiment 2:
Treat a Chinese medicine composition for pollen hypersensitivity, comprise the raw material of following mass fraction: 6 parts of Herba Ephedrae extracts, root of Chinese trichosanthes13 parts of extracts, mix.
The extracting method of described Herba Ephedrae extract is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 4 times of weight, 75 DEG C of heating and refluxing extraction 2 times, each50min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is1.2mL/min, and then with the 1.5mol/L ammoniacal liquor of the 1.0mol/L ammoniacal liquor of 3BV, 3BV and 80% alcohol mixeding liquid, 3BV2.5mol/L ammoniacal liquor and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 1.2mL/min, merge eluent;
(3) eluent reduced pressure concentration, obtains relative density and is 1.08 medicinal extract, is Herba Ephedrae extract.
Wherein, the described ion exchange resin column of step (2) be D113, D155, the series connection of HD-1 type ion exchange resin andBecome, the height of resin column and diameter are than being 4:1.
Wherein, the volume ratio of the filtrate of step (2), D113, D155, HD-1 type ion exchange resin is: 1:0.25:0.25:0.25。
Wherein, the volume ratio of 1.5mol/L ammoniacal liquor and 80% ethanol is 1:3, the volume of 2.5mol/L ammoniacal liquor and 70% ethanolThan being 1:3.
The extracting method of described Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixing of 4 times of weightSolution, 65 DEG C of refluxing extraction 120min, filter to get filtrate, and reduced pressure concentration obtains relative density and is 1.08 medicinal extract, is smallpoxPowder extracts.
Wherein, described mixed solution is 95% ethanol, acetone, n-butanol, and its volume ratio is 1:0.7:0.5.
Embodiment 3:
Treat a Chinese medicine composition for pollen hypersensitivity, comprise the raw material of following mass fraction: 8 parts of Herba Ephedrae extracts, root of Chinese trichosanthes15 parts of extracts, mix.
The extracting method of described Herba Ephedrae extract is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 5 times of weight, 80 DEG C of heating and refluxing extraction 2 times, each60min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is1.5mL/min, and then with the 2.0mol/L ammoniacal liquor of the 1.5mol/L ammoniacal liquor of 4BV, 4BV and 80% alcohol mixeding liquid, 4BV3.0mol/L ammoniacal liquor and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 1.5mL/min, merge eluent;
(3) eluent reduced pressure concentration, obtains relative density and is 1.10 medicinal extract, is Herba Ephedrae extract.
Wherein, the described ion exchange resin column of step (2) be D113, D155, the series connection of HD-1 type ion exchange resin andBecome, the height of resin column and diameter are than being 4:1.
Wherein, the volume ratio of the filtrate of step (2), D113, D155, HD-1 type ion exchange resin is: 1:0.3:0.3:0.3。
Wherein, the volume ratio of 2.0mol/L ammoniacal liquor and 80% ethanol is 1:5, the volume of 3.0mol/L ammoniacal liquor and 70% ethanolThan being 1:5.
The extracting method of described Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixing of 5 times of weightSolution, 70 DEG C of refluxing extraction 140min, filter to get filtrate, and reduced pressure concentration obtains relative density and is 1.10 medicinal extract, is smallpoxPowder extracts.
Wherein, described mixed solution is 95% ethanol, acetone, n-butanol, and its volume ratio is 1:0.9:0.6.
Comparative example 1:
Treat a Chinese medicine composition for pollen hypersensitivity, comprise the raw material of following mass fraction: 9 parts of Herba Ephedrae extracts, root of Chinese trichosanthes10 parts of extracts, mix.
The extracting method of described Herba Ephedrae extract is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 6 times of weight, 60 DEG C of heating and refluxing extraction 2 times, each70min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is2.0mL/min, and then with the 2.5mol/L ammoniacal liquor of the O.3mol/L ammoniacal liquor of 5BV, 1BV and 80% alcohol mixeding liquid, 5BV1.5mol/L ammoniacal liquor and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 0.5mL/min, merge eluent;
(3) eluent reduced pressure concentration, obtains relative density and is 1.04 medicinal extract, is Herba Ephedrae extract.
Wherein, the described ion exchange resin column of step (2) be D113, D155, the series connection of HD-1 type ion exchange resin andBecome, the height of resin column and diameter are than being 4:1.
Wherein, the volume ratio of the filtrate of step (2), D113, D155, HD-1 type ion exchange resin is: 1:0.1:0.4:0.1。
Wherein, the volume ratio of 1.0-2.0mol/L ammoniacal liquor and 80% ethanol is 1:6,2.0-3.0mol/L ammoniacal liquor and 70% secondThe volume ratio of alcohol is 1:6.
The extracting method of described Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixing of 2 times of weightSolution, 80 DEG C of refluxing extraction 90min, filter to get filtrate, and reduced pressure concentration obtains relative density and is 1.12 medicinal extract, is root of Chinese trichosanthesExtract.
Wherein, described mixed solution is 95% ethanol, acetone, n-butanol, and its volume ratio is 1:0.4:0.8.
Comparative example 2:
Treat a Chinese medicine composition for pollen hypersensitivity,
The extracting method of Herba Ephedrae extract is:
The described ion exchange resin column of step (2) is that D113, HD-1 type ion exchange resin are in series, the height of resin columnCompare for 4:1 with diameter.
The volume ratio of the filtrate of step (2), D113, HD-1 type ion exchange resin is: 1:0.2-0.3:0.2-0.3.
All the other are with embodiment 1.
Comparative example 3:
Treat a Chinese medicine composition for pollen hypersensitivity,
The extracting method of Radix Trichosanthis extract is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixed solution of 4 times of weight, 60DEG C refluxing extraction 110min, filters to get filtrate, and reduced pressure concentration obtains relative density and be 1.07 medicinal extract, is Radix Trichosanthis extract.
Wherein, described mixed solution is 95% ethanol, acetone, and its volume ratio is 1:0.5-0.9.
All the other are with embodiment 2.
Embodiment 4:
Adopt conventional preparation technology, mixed extract of the present invention can be made into said peroral dosage form in any pharmacy, asTablet, capsule, granule, pill, syrup.
All the other are with any one in embodiment 1-3.
Embodiment 5:
By the extract mixing being made by embodiment 1-3, according to the method for operating of conventional tablet, add pharmaceutic adjuvant,Each component is mixed, and wet granulation, sieves, and adopts conventional tablet preparation method compressing tablet to make tablet, wherein containing of each componentMeasure as follows:
Composition weight (part)
Mixed extract 50
Dextrin 25
Starch slurry 20
Sodium carboxymethyl starch 10
Talcum powder 12
Polyethylene glycol 8
All the other are with any one in embodiment 1-3.
Embodiment 6:
By the extract mixing being made by embodiment 1-3, according to the method for operating of conventional capsule, add medicinal auxiliaryMaterial, mixes each component, granulates, and adopts conventional capsule preparation method to fill and makes capsule, the wherein content of each componentAs follows:
Composition weight (part)
Mixed extract 100
Soluble starch 62
Dolomol 21
Low-substituted hydroxypropyl cellulose 16
Sodium chloride 8
All the other are with any one in embodiment 1-3.
Embodiment 7:
By the extract mixing being made by embodiment 1-3, according to the method for operating of conventional syrup, ethanol precipitation 1-2Inferior, filter, concentrated, aqueous precipitation, filters, and adds pharmaceutic adjuvant, filters, and packing sterilizing, adopts conventional syrup to prepare legal systemStandby oral liquid, wherein the content of each component is as follows:
Composition weight (part)
Mixed extract 100
Ethanol 120
Sucrose 80
Sodium carboxymethylcellulose 15
Sorbic acid 4
Benzoic acid 3
All the other are with any one in embodiment 1-3.
Animal efficacy experiment:
1 animal
Kunming mouse (Zhejiang College Of Traditional Chinese Medicine Animal House); SD big white mouse (Zhejiang Academy of Medical Sciences Experimental Animal Center); GlobefishMouse (Zhejiang Medical university Experimental Animal Center).
2 reagent
Hydrochloric acid Cyproheptadine sheet (red flag pharmaceutical factory of Shanghai Medical Univ, 2mg/ sheet); Codeine phosphate tablets (Qinghai Pharmaceutic Plant, 2mg/Sheet); Histamine phosphate (Chinese Academy of Sciences Shanghai east wind Biochem Technology, INC. of biochemical institute); Acecoline (Shanghai biological chemical reagentCompany); Hundred, white, broken triple vaccine (health and epidemic prevention station of Zhejiang Province); Evans blue (Solution on Chemical Reagents in Shanghai purchasing and supply station); OvumAlbumin (Shanghai biological chemical reagent company).
4 instruments
402 ultrasonic atomizers (Shanghai make a concerted effort medical apparatus and instruments factory); 722-grating spectrophotometer (Shanghai the 3rd analytical instrument factory).
5 on causing the impact of mouse cough
Get Kunming mouse, male, body weight 18 ± 2g, puts into glass bell jar one by one, draws to cough that (mist amount is high with 25% ammonia sprayingClass) 15s, observes mouse and starts to occurring that abdominal muscle shrinks the time of opening one's mouth, and does not occur in 1.5min that cough person discards from spraying.Select responsive mouse and be divided at random 8 groups, 8 every group: embodiment 1-3 group, comparative example 1-3 group, codeine group, physiological saline contrastGroup, respectively organizes dosage: embodiment 1-3 group, comparative example 1-3 group are all 100mg/kg, codeine group 30mg/kg, and above medicine is equalBe mixed with 0.2ml/ gavage, control group gives equal-volume physiological saline. Continuously after 2d, then before method draw and cough, observe each group and coughCough incubation period (table 1).
Because embodiment 1-3 is in processing range of the present invention, comparative example 1 has changed technological parameter, and comparative example 2 has changedThe extraction process (adopting different ions exchanger resin) of Herba Ephedrae extract, the extraction process that comparative example 3 has changed root of Chinese trichosanthes (adoptsDifferent mixed solutions extract).
As shown in Table 1, the cough that embodiment 1-3 causes ammoniacal liquor has significant inhibitory action, is obviously better than comparative example 1-3Group and codeine group.
6 antiasthmatic effects
6.1 on acetylcholine and histamine, spraying causes the impact of Experimental Asthma In Guinea-pigs: get 48 of the cavys of body weight 200g left and right, male and female are equalCan, be divided at random 8 groups, 6 every group: embodiment 1-3 group, comparative example 1-3 group, cyproheptadine group, physiological saline group. Dosage:Embodiment 1-6 is 100mg/kg, 50mg/kg, cyproheptadine group 2.65mg/kg. Only all be diluted to 3ml/, gavage, control group is givenEqual-volume physiological saline. Successive administration 3d, 1h after last administration, is placed in respectively in the seal glass cover of 4L volume, with maximumMist amount class sprays 2% acetylcholine and 0.1% histamine equivalent mixed liquor, and 15s sprays at every turn. After spraying stops, observing cavy and occurPanting property is twitched, the incubation period (table 2) of overturning.
Because embodiment 1-3 is in processing range of the present invention, comparative example 1 has changed technological parameter, and comparative example 2 has changedThe extraction process (adopting different ions exchanger resin) of Herba Ephedrae extract, the extraction process that comparative example 3 has changed root of Chinese trichosanthes (adoptsDifferent mixed solutions extract).
As shown in Table 2, embodiment 1-3 can significant prolongation Experimental Asthma In Guinea-pigs incubation period, be obviously better than comparative example 1-3 group and match heptanPyridine group.
7 impacts (PCA) on mouse ear xenogenesis models of passive skin irritability
7.1 antiserum preparations: get SD big white mouse, male, body weight 200g left and right, every mouse two back leg intramuscular injection 1% ovalbumin physiology saltWater 0.5ml, meanwhile, lumbar injection PertussisDiphtheriaTetanus triple vaccine (2 × 1010 thalline), every mouse 1ml, after 14d, broken end is got blood, centrifuging and takingAntiserum, put-4 DEG C following for subsequent use.
7.2 sensitization and antigen are attacked: get 80 of Kunming kind small white mouses, and male, body weight 18 ± 2g, every mouse two auricles are each subcutaneous(l) sensitization of every ear 20 μ, is then divided into 8 groups, 10 every group at random to inject large Mouse Antisera 40 μ l; If physiological saline group, embodiment1-3 group, comparative example 1-3 group, cyproheptadine group; Each group is pressed different pharmaceutical and dosage gavage respectively, embodiment 1-3 group, comparative example 1-3Group is all 50mg/kg, cyproheptadine group 2.65mg/kg, once a day. For the first time after gavage and sensitization 48h before half an hour be lastGavage, carries out antigen attack after half an hour, by tail vein injection 0.25ml5% Evans blue solution (including ovalbumin 1%),Inject successively control group, embodiment 1-3 group, comparative example 1-3 group, cyproheptadine group, after half an hour, by injection sequence, mouse put to death,Cut two auricles (indigo plant is dyed position), shred and be placed in vitro, add 6ml acetone-physiological saline (7: 3), fully shake up, soak48h, centrifugal 15min, gets supernatant, measures optical density (OD) with 610nm wavelength, calculates the inhibition hundred of administration group to PCA reactionDivide rate, its computing formula is as follows.
Inhibition percentage=(control group optical density-medication group optical density)/control group optical density × 100%
7.3 the results are shown in Table 3.
Because embodiment 1-3 is in processing range of the present invention, comparative example 1 has changed technological parameter, and comparative example 2 has changedThe extraction process (adopting different ions exchanger resin) of Herba Ephedrae extract, the extraction process that comparative example 3 has changed root of Chinese trichosanthes (adoptsDifferent mixed solutions extract).
As shown in Table 3, embodiment 1-3 inhibiting rate is obviously better than comparative example 1-3 group and cyproheptadine group.
Brief summary: be all obviously better than 1 group of (technological parameter of comparative example for irritated result for the treatment of at embodiment of the present invention 1-3Different), comparative example 2 groups of (the extraction process difference of Chinese ephedra), comparative example 3 groups of (the extraction process difference of root of Chinese trichosanthes) and cyproheptadinesGroup, the active ingredient that the extraction process of visible technological parameter, Chinese ephedra, the extraction process of root of Chinese trichosanthes are all extracted the medicine making andCurative effect has impact, and therefore the present invention has adopted the extraction process of specific technological parameter, Chinese ephedra and root of Chinese trichosanthes, just can make curative effectThe medicine of significant treatment pollen hypersensitivity.
Claims (10)
1. a Chinese medicine composition for the treatment of pollen hypersensitivity, is characterized by: comprise raw material: Chinese ephedra, root of Chinese trichosanthes.
2. a Chinese medicine composition for the treatment of pollen hypersensitivity, is characterized by: the raw material that comprises following mass fraction: Herba Ephedrae extract4-8 part, Radix Trichosanthis extract 11-15 part, mixes.
3. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 2, is characterized by: the extraction of Herba Ephedrae extractMethod is:
(1) Chinese ephedra is pulverized, cross 10 mesh sieves, add 50% ethanol of 3-5 times of weight, 70-80 DEG C of heating and refluxing extraction 2 times, each40-60min, merges extract, filters, and obtains filtrate;
(2) regulating filtrate pH with the NaOH solution of 0.5mol/L is 9-10, then filtrate is passed through to ion exchange resin column, and flow velocity is1-1.5mL/min, and then with the O.5-1.5mol/L ammoniacal liquor of 2-4BV, 1.0-2.0mol/L ammoniacal liquor and 80% ethanol of 2-4BVThe 2.0-3.0mol/L ammoniacal liquor of mixed liquor, 2-4BV and 70% alcohol mixeding liquid carry out wash-out successively, and elution flow rate is 1-1.5mL/Min, merges eluent;
(3) eluent reduced pressure concentration, obtains the medicinal extract that relative density is 1.05-1.10, is Herba Ephedrae extract.
4. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 3, is characterized by: step (2) described fromSub-exchange resin post is that D113, D155, HD-1 type ion exchange resin are in series, and the height of resin column and diameter are than being 4:1.
5. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 3, is characterized by: the filtrate of step (2),The volume ratio of D113, D155, HD-1 type ion exchange resin is: 1:0.2-0.3:0.2-0.3:0.2-0.3.
6. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 3, is characterized by: 1.0-2.0mol/L ammoniaThe volume ratio of water and 80% ethanol is 1:1-5, and the volume ratio of 2.0-3.0mol/L ammoniacal liquor and 70% ethanol is 1:1-5.
7. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 2, is characterized by: the carrying of Radix Trichosanthis extractAccess method is: root of Chinese trichosanthes is pulverized and crossed 20 mesh sieves, add the mixed solution of 3-5 times of weight, 60-70 DEG C of refluxing extraction 100-140min, filters to get filtrate, and reduced pressure concentration obtains the medicinal extract that relative density is 1.05-1.10, is Radix Trichosanthis extract.
8. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 7, is characterized by: described mixed solution is95% ethanol, acetone, n-butanol, its volume ratio is 1:0.5-0.9:0.4-0.6.
9. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 2, is characterized by: after mixing, make and appointSaid peroral dosage form in what a kind of pharmacy.
10. a kind of Chinese medicine composition for the treatment of pollen hypersensitivity as claimed in claim 9, is characterized by: described peroral dosage form is:Tablet, capsule, granule, pill, syrup.
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Cited By (1)
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Application publication date: 20160518 |