CN105581014A - Fermented feed-containing 5% laying hen premix and laying hen full price daily diet prepared from the premix - Google Patents
Fermented feed-containing 5% laying hen premix and laying hen full price daily diet prepared from the premix Download PDFInfo
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Abstract
The present invention discloses a fermented feed-containing 5% laying hen premix and a laying hen full price daily diet prepared from the premix. The fermented feed consists of corn, tenebrio molitor meal, soybean meal, cottonseed meal, apple residues, chili meal, corn soaking liquid sprayed corn husk, wheat bran, microorganism liquid and water. The preparation steps of the fermented feed are as follows: raw material crushing and weighing, raw material primary mixing, strain activating, strain and raw material secondary mixing, solid anaerobic fermenting, and wet basic state fermentation product drying. The fermented feed-containing 5% laying hen premix comprises dry basic state fermentation feed and other materials. The daily diet consists of corn, soybean meal, stone powder, and the fermented feed-containing 5% laying hen premix. The advantages of the daily diet are that after the laying hens are fed by the daily diet, the laying hen egg production rate and other egg laying performances and egg product quality are significantly improved, egg production peak period is extended, immune function is enhanced, anti-stress ability is improved, and the daily diet can superimposedly play the roles of regulating the microecology of beneficial bacterium flora and regulating neurotransmitter in the central nervous system.
Description
Technical field
The present invention relates to field of feed, be specifically related to a kind of 5% laying hen premix containing fermented feed andThe daily ration being mixed with by it.
Background technology
Laying hen enteron aisle is shorter, and chyme is fast by enteron aisle speed, average 4-6 hour, and nutriment can not get fullyDigest and absorb; Residual for preventing the deposition of medicine in egg product, laying cycle of laying hens was forbidden in dayIn grain, add feeding antibiotic, laying hen should have higher immunity function with the infection of opposing various diseases and weighThe invasion and attack of large epidemic disease; Laying cycle of laying hens is very long, lasts till 80 week age from 20 week age, and it is vigorous that laying hen should keepFunctional state is to maintain longer laying eggs rush hour; Laying hen is timid easily frightened, each to trophic level, environment etc.The change of kind of factor all easily produce stress, especially, in hot environment, because of laying hen fever, metabolism is strongDegree is large, and skin lacks sweat gland, and the thick feather of coating one deck, and the suitable environment temperature of layer breeding is15～25 DEG C, when temperature reaches 30 DEG C, mainly unnecessary by what produce in respiratory tract evaporative heat loss mode releaserHeat carrys out balance body temperature, can produce serious heat stress, and the egg laying performances such as laying rate significantly decline, rate of broken eggsBe a greater impact Deng Egg Quality.
According to the above-mentioned nutrition physiology of laying hen, digestive physiology, immunity and stress wait feature, current, in laying hen dayIn grain, normal employing to add the technical measures of probiotics as main several functions active factors, to regulateThe functional state of laying hen entirety.
Dry powder-shaped probiotics is applied as the most general on laying hen produces, but conventionally only containing bacillus oneBeneficial bacterium, not containing lactic acid bacteria and saccharomycete, because lactic acid bacteria is anaerobic bacteria, easy inactivation in air, application is notJust, also nonhost bacterium of saccharomycete. In addition, the addition of dry powder-shaped probiotics in layer diets is less,Mostly be 100～400g/t, if bacillus is added 200g/t, because of addition is little again can not be in enteron aisle default value,Micro-Ecology Actions such as its performance colony balance adjusting are very limited. And dry powder-shaped probiotics is not containing bacteriumThe active metabolite of body, single dependence beneficial bacterium itself, is difficult to play regulation and control effect.
Wet basis state fermentative feedstuff of microbe is a kind of biological feedstuff of high-quality, the beneficial bacterium that can provide high-load to liveAnd microbial activity metabolite, as: lactic acid, enzyme, immune enhancer, unknown somatomedin, if withBe added in a large number at high proportion in layer diets, will produce the collection of very significant micro-ecology and activated product thereofBecome regulating and controlling effect. But, because wet basis state fermented feed contains 30～45% high-load moisture, make on the one handThe content of nutritional labeling reduces relatively, and especially metabolizable energy is lower; On the other hand with daily ration in other feedRaw material mix time, easily caking, can not fully mix, and increase labour, be difficult for transport, be inconvenient to makeWith, therefore, its application is subject to larger restriction with popularization.
Wet basis state microecological fermented forage is dried, moisture is reduced to below 15%, be convenient to transport,Application, but multiplex thermal source in the stoving process adopting, bake out temperature is 140 DEG C～250 DEG C, in fermented feedThe functional activity such as lactic acid bacteria and the enzyme factor after high temperature action, activity is subject to heavy damage, Ji HuquanPortion's loss.
Laying hen premix and be laying hen with one of principal mode of daily ration by the layer diets of its preparation. At present,In commercially available 5% laying hen premix product, do not add probiotics, or interpolation is equivalent to be folded in complete feed100-400g/t bacillus, be still difficult to see fermented type laying hen premix product.
It is particularly convenient that laying hen premix is used, and only need mix with the raw material such as corn, dregs of beans of corresponding proportioningBe mixed with complete feed, can apply. The development and the fermented feed that how to solve functional fermented feed are multipleThe use efficacy problems being fitted on after premix becomes key problem in technology place.
Summary of the invention
Main purpose of the present invention is to provide a kind of 5% laying hen premix containing fermented feed and the laying hen being mixed with thereofAdequate diet. This fermented feed by a kind of functional lactobacillus of uniqueness and other two kinds of beneficial bacteriums to specificallyRaw material carries out anaerobic solid-state fermentation, low temperature drying and obtains, and its form is butt state, contains a large amount of preservations completeThe metabolic activity factor of the good multiple thalline such as viable bacteria and lactic acid; Then with larger proportion and trace element premixClose feed, vitamin premixed feed, amino acid feed additive etc. and be mixed with 5% premix. Feed by thisThe complete feed daily ration of the large raw material preparation of 5% premix and corresponding proportion, the egg laying performances such as laying rate of laying hen and chickenEgg product quality is significantly improved, and egg-laying peak extends, and immunity function strengthens, and anti-stress ability improves,Breeding layer chicken ecology, health, welfare.
The technical scheme that the present invention realizes its object employing is:
Containing a 5% laying hen premix of fermented feed, it is characterized in that, by 1000 parts of gross weights, bagDraw together: 30～50 parts of trace element premixing feeds, 4.5～5.5 parts of vitamin premixed feeds, vitamin C 1.0～3.0 parts, 230～320 parts of stone flours, 120～180 parts of calcium monohydrogen phosphates, 2.0～3.0 parts of complex enzymes, phytase2.0～3.0 parts, 15～30 parts of DL-METHIONINEs, 2.0～5.0 parts of betaines, 50～70 parts of salt, chlorination courage15～25 parts, alkali (containing choline 50%), 5.0～20 parts, sodium bicarbonate, 5.0～20 parts, potassium chloride, butt fermentation150～400 parts, feed, 0.5～1.5 part of ethoxyquinoline, 0～400 part of zeolite powder.
As preferred technical scheme, by 1000 parts of gross weights, the group of described trace element premixing feedBecome: 140～200 parts of ferrous sulfate monohydrates, 15～60 parts of cupric sulfate pentahydrates, monohydrate zinc sulphate 120～200Part, 140～200 parts of manganese sulfate monohydrates, 15～25 parts of water sodium selenites, 15～40 parts of calcium iodates, chlorineChange 5.0～40 parts of cobalts, 0.5～1.5 part of ethoxyquinoline, 233.5～549.5 parts of zeolite powders.
Wherein the effective content of above-mentioned trace element premixing feed each component is as follows: in ferrous sulfate monohydrate, containIron 30%, cupric 25% in cupric sulfate pentahydrate, containing zinc 35%, contains manganese in monohydrate zinc sulphate in manganese sulfate monohydrateIn 32%, one water sodium selenite, containing selenium 1%, in calcium iodate, containing iodine 1%, in cobalt chloride, contain cobalt 1%, ethyoxylThe mass fraction of quinoline is 30%.
As preferred technical scheme, by 1000 parts of gross weights, the composition of described vitamin premixed feedFor: 50～80 parts of retinyl acetates, vitamin D315～30 parts, 20～200 parts of vitamin e1s,5.0～15 parts of menadione sodium bisulfites, 6.0～10 parts of thiamine hydrochlorides, 5～45 parts of Riboflavin Tetrabutyrates, hydrochloric acid13～20 parts of pyridoxols, Cobastab124.0～8.0 parts, 35～60 parts of D-VB5 calcium, niacinamide 90～125Part, 3.5～6.0 parts, folic acid, 25～40 parts of Bios, 0.5～1.5 part of ethoxyquinoline, powdered rice hulls 359.5～608.0 part.
The effective content of said vitamin premixed feed each component is as follows: retinyl acetate and support one's familyElement D3Biological value is 500,000 IU/g, and the mass fraction of vitamin E is 50%, sodium hydrogensulfite first naphthaleneThe mass fraction of quinone is 50%, and the mass fraction of thiamine hydrochloride is 98%, and the mass fraction of riboflavin is 80%,The mass fraction of puridoxine hydrochloride is 98%, Cobastab12Mass fraction be 1%, the quality of D-VB5 calciumMark is 98%, the mass fraction of niacinamide is 99%, and the mass fraction of folic acid is 98%, D-bionic acidMass fraction is 2%, and the mass fraction of ethoxyquinoline is 30%.
As preferred technical scheme, by 1000 parts of gross weights, the consisting of of described complex enzyme: xylan700 parts of enzymes, 100 parts of cellulases, 50 parts of 1,4 beta-glucanases, 50 parts of pectases, 100 parts of maize cob meals.
Wherein the activity of above-mentioned complex enzyme each component is as follows: the activity of various enzymes is: zytase 10000U/g,Cellulase 10000U/g, 1,4 beta-glucanase 2000U/g, pectase 2000U/g.
As preferred technical scheme, described butt fermented feed by wet basis state fermented feed through low temperature drying systemStandby obtaining,
And by 1000 parts of gross weights, described wet basis state fermented feed consists of: 30～100 parts of corns, HuangMealworm powder 50-120 part, 0～150 part of dregs of beans, 50～150 parts of Cottonseed Meals, 40～120 parts of pomaces, peppery50～100 parts of the green pepper dregs of rice, 40～120 parts of maize peels of whitewashing, 100～300 parts of wheat brans, bacterium liquid 40～60200～300 parts, part, water.
As preferred technical scheme, by 1000 parts of gross weights, the consisting of of described bacterium liquid: enterococcus faecalisThe mixture of 5～15 parts of F6 (EnterococcusfaeciumF6), bacillus subtilis and bacillus licheniformis2.5～7.5 parts, 2.5～7.5 parts of S. cervisiaes, 20～50 parts, brown sugar, 920～970 parts, water.
As preferred technical scheme, withered grass in the mixture of described bacillus subtilis and bacillus licheniformisThe ratio of bacillus and bacillus licheniformis is 1: 1.
The above-mentioned method of being prepared by wet basis state fermented feed low temperature drying to butt fermented feed: the fermentation of wet basis state is raisedMaterial is through hopper feeding flash dryer; Steam heater produces hot-air, and temperature is no more than 100 DEG C; Carry out heatWind is dried; Control discharging opening hot blast temperature and be no more than 80 DEG C, obtain butt fermented feed;
The preparation method of wherein said wet basis state fermented feed: impurity removing, sieve with 1.5～2.8mm after weighingPulverize in hole; Raw material after pulverizing is carried out to mixed once 2～5 minutes; Bacterial classification activates in 30～45 DEG C of water temperatures0.5～1.0h; Bacterium liquid after activation be pulverized and mixed after raw material mix again; Packed under 20 DEG C～45 DEG C conditionsSolid anaerobic digestion 5～10 days.
The Major Nutrient level of the above-mentioned above-mentioned wet basis state fermented feed preparing is: moisture 32～42%,Metabolizable energy 1350～1850kcal/kg, protein 13～20%, calcium 0.20～0.70%, total phosphorus 0.50～0.80%,Lysine 0.40～1.10%, methionine 0.10～0.45%, threonine 0.30～0.80%, tryptophan 0.10～0.30％。
Other active component content in the above-mentioned wet basis state fermented feed preparing is: GABA0.20～0.45g/kg, enterococcus faecalis is containing viable bacteria 1.0 × 108～2.5×108Cfu/g, total viable count 2.5 × 108～7.5×108cfu/g。
The Major Nutrient level of the described butt fermented feed making is: moisture 13～16%, and metabolizable energy 1900～2450kcal/kg, protein 16～30%, calcium 0.35～0.90%, total phosphorus 0.60～0.95%, lysine 0.70～1.55%, methionine 0.20～0.55%, threonine 0.40～0.95%, tryptophan 0.15～0.40%, gamma-aminoButyric acid 0.22～0.50g/kg, enterococcus faecalis is containing viable bacteria 0.7 × 108～1.7×108Cfu/g, total viable count 2.0 ×108～6.0×108cfu/g。
The Major Nutrient level of the above-mentioned 5% laying hen premix containing fermented feed making is: moisture 7.0～13%, metabolizable energy 200～800kcal/kg, protein 4.0～10%, calcium 11～15%, total phosphorus 2.4～3.0%,Lysine 0.20～0.45%, methionine 1.8～2.5%, threonine 0.14～0.30%, tryptophan 0.05～0.10%.
Above-mentioned 5% laying hen premix and other the large raw materials containing fermented feed making is mixed with to laying hen full price dayGrain: by 1000 parts of gross weights, comprising: 580～660 parts of corns, 220～280 parts of dregs of beans, stone flour 80Part, and 50 parts of above-mentioned 5% laying hen premixes containing fermented feed.
The Major Nutrient level of the above-mentioned daily ration making is: moisture 10～14%, metabolizable energy 2500～2800Kcal/kg, protein 14.5～17.5%, calcium 3.2～4.0%, total phosphorus 0.40～0.70%, lysine 0.70～0.92%,Methionine 0.31～0.41%, threonine 0.50～0.75%, tryptophan 0.15～0.20%.
Bacillus subtilis and bacillus licheniformis are respectively containing viable bacteria 30,000,000,000 cfu/g and 20,000,000,000 cfu/g, by 1:The mixed viable bacteria content of 1 ratio is 25,000,000,000 cfu/g; S. cervisiae is containing viable bacteria 200,000,000 cfu/g.
In addition, the employing low temperature drying wet basis state fermented feed of mentioning is in the present invention prepared butt fermented feedMethod, i.e. expansion drying method, specific as follows:
(1) charging: the hopper feeding by wet basis state fermented feed from flash dryer, defeated through screw feederDelivering to the stirring of main frame pulverizes in hothouse, by buncher control material transfer rate.
(2) hot blast produces: open hot-air system, make steam heater produce hot-air, control entrance hot blastTemperature is no more than 100 DEG C.
(3) hot-air seasoning: hot blast through connect airduct, by inlet tube with suitable spouted speed from drying machine machineBottom enters to stir pulverizes hothouse, and wet basis state fermented feed is produced strong shearing, blows floating, rotates workWith, so that fermentation material is subject to is centrifugal, cut joint, collision, friction and by micronize, enhancing mass and heat transfer. DryDry motor spindle, large wetter fermented feed agglomerate under agitator effect by Mechanical Crushing, humidity and particleSpend less particle and be rotated air-flow and carry rising secretly, further dry in uphill process.
(4) discharging: the hot blast temperature of controlling discharging opening is no more than 80 DEG C. Dried material is through cyclonic separationAfter device separates, discharge through Blowing stopper, obtain the butt fermented feed for laying hen premix, it is directBe packaged in the packaging bag of surface coating.
(5) tail gas is discharged: the tail gas of generation is discharged through air-introduced machine after by second cyclone dust extractor dedusting.
The preparation method of wet basis state fermented feed is provided in the present invention, comprise pulverizing, mixing, actication of culture,Mix again, the step such as fermentation, specific as follows:
(1) raw material is pulverized: raw material corn, dregs of beans, Cottonseed Meal, pomace, the capsicum dregs of rice that needs are pulverized,Whitewashing maize peel, wheat bran screen out decontamination by reciprocating vibration first respectively, then computation weigh,The beater disintegrating machine that is 1.5～2.8mm with sieve diameter is again pulverized.
(2) mixed once: use bucket elevator vertical-lift to storehouse to be mixed raw material after above-mentioned pulverizing,Then putting into horizontal-type biaxial paddle type mixer mixes 2～5 minutes.
(3) actication of culture: by enterococcus faecalis, bacillus, S. cervisiae and brown sugar, water according to above-mentionedThe ratio of regulation stirs and evenly mixs, and makes bacterial classification activate 0.5～1.0h in 30～45 DEG C of water temperatures.
(4) mix again: the bacterium liquid after activation is delivered to mixer via closed conduit, by 3-6Tiny spray is empty to be sprayed onto in the material of mixer, and mix 2.0～5.0 minutes on sprinkling limit, limit, after bacterium liquid been sprayed,Mix again 1.5～3.0 minutes.
(5) fermentation: adopt packed solid anaerobic digestion mode, the material warp after above-mentioned inoculation bacterium liquid is mixedDischarging opening connects material, and is packaged in liner some strength and flexible film inner bag, outer being wrapped in coated knitted bag;Discharge air in bag, tighten sack; Then anaerobic fermentation 5～10 days under 20 DEG C～45 DEG C conditions, i.e. systemStandby one-tenth wet basis state fermented feed
It should be noted that, in the present invention, enterococcus faecalis F6 (EnterococcusfaeciumF6)For applicant oneself is through separating, cultivate gained in fermentation medium, and applicant is in 2013 12The moon is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on the 9th, is called for short CGMCC,Address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, preservation is compiledNumber: CGMCCNo.8538. The output of this bacterial strain GABA that cultivation obtains reaches 7.1g/L, utilizesSeed selection obtains the functional characteristic of the highly producing gamma-aminobutyric acid of this bacterium, and by solid anaerobic digestion, tunning is held concurrentlyThe beneficial bacterium lactic acid bacteria of tool high-load and neurotransmitter GABA, stack is brought into play its uniqueness and is resisted significantlyHeat stress effect. Enterococcus faecalis F6 (EnterococcusfaeciumF6) is containing viable bacteria 500,000,000 cfu/g.
The beneficial effect that the present invention has:
(1) in the wet basis state fermented feed obtaining by three kinds of beneficial bacterium solid anaerobic digestion, total viable count 2.5×108～7.5×108Cfu/g, enterococcus faecalis is containing viable bacteria 1.0 × 108～2.5×108Cfu/g; Wet basis state fermented feedThe butt fermented feed obtaining through low temperature drying, total viable count 2.0 × 108～6.0×108Cfu/g, excrement intestines ballBacterium is containing viable bacteria 0.7 × 108～1.7×108Cfu/g. Therefore, butt fermented feed viable bacteria content is high, enterococcus faecalisLittle Deng lactic bacteria activity loss, compared with not containing or seldom contain lactic acid bacteria in routine oven dry fermented feed, becomeMerit solves the difficult problem that heat labile anaerobic bacteria lactic acid bacteria is difficult for application.
(2) in butt fermented feed, still contain highly active bacterial metabolism product, as: lactic acid, amylase,Protease, non-starch polysaccharide enzyme, B family vitamin, nucleotides, bacteriocin, antibacterial peptide, unknown growth promotion because ofSons etc., its regulating and controlling effect of bringing into play micro-ecology is far above using the single probiotics that only contains viable bacteria.
(3) butt fermented feed adds with 10～30%, is mixed with the 5% laying hen premix containing fermented feed,Be mixed with layer diets by this premix again, because butt fermented feed adding proportion is large, be folded to full price dayRatio in grain is 0.5～1.5%, and the beneficial bacterium adding in common 5% laying hen premix is folded to full price dayRatio in grain is often 0.01～0.04%, therefore, and the daily ration of feeding and preparing containing the laying hen premix of fermented feed,The improvement of Layer Production Performance is more remarkable.
(4) adopted the enterococcus faecalis of the product GABA of a strain unique function to carry out fermentation raw material, made to doAlpha-aminobutyric acid content in ground state fermented feed reaches 0.22～0.50g/kg, and conventional wet basis state fermented feed,In butt fermented feed and 5% laying hen premix, contain hardly GABA.
(5) adequate diet of feeding and preparing containing 5% laying hen premix of fermented feed, the micro-life of laying hen intestines and stomachState colony balance improves, and nutrient digestion absorption rate improves, and immunity function strengthens, the entirety of bodyFunctional status is vigorous, shows as: laying rate of laying hen improves 2.0～7.0%, and feedstuff-egg ratio is reduced to 1.98～2.12,Egg-laying peak extends to 8 months, suffers from great epidemic disease Risk Reduction, and hen house ammonia concentration declines more than 40%,The anti-heat stress ability that especially high temperature resistance causes strengthens, breeding layer chicken health, welfare.
(6) preparation of wet basis state fermented feed and butt fermented feed adopts strict, standardized technique barPart, fermented product steady quality.
(7) be powder-form containing 5% laying hen premix of butt fermented feed, only need with corn, dregs of beans,Three kinds of raw materials of stone flour, mix by a certain percentage and are mixed with adequate diet, can feed, and therefore use outstandingFor convenience.
(8) containing 5% laying hen premix of butt fermented feed and common 5% laying hen premix cost also phase comparativelyClosely.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but protection scope of the present invention is not only limited toIn embodiment.
Embodiment 1
A kind of wet basis state fermented feed that the present embodiment provides, by 1000 parts of gross weights, by following raw material groupBecome:
60 parts of corns, 80 parts, yellow meal worm powder (adult polypide), 50 parts of dregs of beans, 100 parts of Cottonseed Meals, apple80 parts of pomaces, 80 parts of the capsicum dregs of rice, 80 parts of maize peels of whitewashing, 170 parts of wheat brans, 50 parts of bacterium liquid, water250 parts.
In the present embodiment, described bacterium liquid composition: by 1000 parts of gross weights, 10 parts of enterococcus faecalis, withered grass bud5.0 parts, spore bacillus and bacillus licheniformis mixture (both ratios 1: 1), 5.0 parts of S. cervisiaes, red35 parts of sugar, 945 parts, water. Enterococcus faecalis is containing viable bacteria 500,000,000 cfu/g, bacillus subtilis and bacillus licheniformisContaining viable bacteria 30,000,000,000 cfu/g and 20,000,000,000 cfu/g, be 250 in 1: 1 mixed viable bacteria content of ratio respectivelyHundred million cfu/g; S. cervisiae is containing viable bacteria 200,000,000 cfu/g.
The Major Nutrient level of above-mentioned wet basis state fermented feed prepared by the present embodiment is: moisture 38.5%, metabolismEnergy 1539kcal/kg, protein 16.1%, calcium 0.46%, total phosphorus 0.65%, lysine 0.78%, methionine0.24%, threonine 0.53%, tryptophan 0.20%, GABA 0.35g/kg, enterococcus faecalis is containing viable bacteria 1.5×108Cfu/g, total viable count 4.0 × 108cfu/g。
Wet basis state fermented feed in the present embodiment is dried to moisture by low temperature drying method of the present invention and be14.0%, the Major Nutrient level of the butt fermented feed obtaining is: moisture 14.0%, and metabolizable energy 2151kcal/kg,Protein 22.4%, calcium 0.64%, total phosphorus 0.90%, lysine 1.20%, methionine 0.34%, threonine 0.74%,Tryptophan 0.27%, GABA 0.39g/kg. Enterococcus faecalis is containing viable bacteria 1.0 × 108Cfu/g, total viable count3.3×108cfu/g。
A kind of 5% laying hen premix containing fermented feed providing in the present embodiment, by gross weight] 000 part,Formed by following raw material: 40 parts of trace element premixing feeds, 5.0 parts of vitamin premixed feeds, support one's family(90%) 2.0 part of element C, 270 parts of stone flours, 140 parts of calcium monohydrogen phosphates, 2.5 parts of complex enzymes, phytase 2.5Part, 21 parts of DL-METHIONINEs, 3.0 parts of betaines, 60 parts of salt, Choline Chloride (containing choline 50%) 20Part, 10 parts, sodium bicarbonate, 10 parts, potassium chloride, 280 parts, butt fermented feed, 1.0 parts of ethoxyquinolines, boil133 parts of stone flours.
Wherein, described trace element premixing feed composition: by 1000 parts of gross weights, ferrous sulfate monohydrate170 parts, 25 parts of cupric sulfate pentahydrates, 150 parts of monohydrate zinc sulphates, 170 parts of manganese sulfate monohydrates, a water selenous acid20 parts, sodium, 25 parts of calcium iodates, 10 parts of cobalt chlorides, 1.0 parts of ethoxyquinolines, 429 parts of zeolite powders.
Wherein, described vitamin premixed feed composition: by 1000 parts of gross weights, retinyl acetate70 parts, vitamin D320 parts, 60 parts of vitamin e1s, 10 parts of menadione sodium bisulfites, thiamine hydrochloride8.0 parts of elements, 35 parts, riboflavin, 16 parts of puridoxine hydrochlorides, Cobastab126.0 parts, D-VB5 Ca45 part,105 parts of niacinamide, 4.5 parts, folic acid, 32 parts of Bios, 1.0 parts of ethoxyquinolines, powdered rice hulls 487.5Part.
The Major Nutrient level of a kind of 5% laying hen premix containing fermented feed providing in the present embodiment is: waterDivide 11.2%, metabolizable energy 622kcal/kg, protein 8.5%, calcium 13.2%, total phosphorus 2.7%, lysine 0.34%,Methionine 2.17%, threonine 0.20%, tryptophan 0.07%.
The present embodiment provides the laying hen by the above-mentioned preparation of the raw material such as 5% laying hen premix and corn containing fermented feedDaily ration, by gross weight umber 1000, composed as follows: 620 parts of corns (crude protein 8.0%), dregs of beans is (thickProtein 43 .5%) 250 parts, 80 parts of stone flours, containing 50 parts of 5% laying hen premixes of fermented feed.
The Major Nutrient level of the layer diets that the present embodiment is made into is as follows: moisture 12.1%, metabolizable energy 2631Kcal/kg, protein 16.3%, calcium 3.76%, total phosphorus 0.43%, lysine 0.83%, methionine 0.38%,Threonine 0.62%, tryptophan 0.17%.
Embodiment 2
A kind of wet basis state fermented feed that the present embodiment provides, by 1000 parts of gross weights, by following raw material groupBecome:
40 parts of corns, 50 parts, yellow meal worm powder (adult polypide), 0 part of dregs of beans, 80 parts of Cottonseed Meals, apple100 parts of slags, 100 parts of the capsicum dregs of rice, 100 parts of maize peels of whitewashing, 230 parts of wheat brans, 40 parts of bacterium liquid,260 parts, water.
In the present embodiment, described bacterium liquid composition: by 1000 parts of gross weights, 500,000,000 cfu/g enterococcus faecalis 12Part, 4.0 parts of 30,000,000,000 cfu/g bacillus subtilises and 20,000,000,000 cfu/g bacillus licheniformis (1: 1), 250Hundred million 4.0 parts of cfu/g S. cervisiaes, 30 parts, brown sugar, 950 parts, water.
The Major Nutrient level of above-mentioned wet basis state fermented feed prepared by the present embodiment is: moisture 38.7%, metabolismEnergy 1459kcal/kg, protein 13.2%, calcium 0.34%, total phosphorus 0.60%, lysine 0.58%, methionine0.19%, threonine 0.41%, tryptophan 0.16%, GABA 0.38g/kg, enterococcus faecalis is containing viable bacteria 1.2×108Cfu/g, total viable count 3.5 × 108cfu/g。
Wet basis state fermented feed in the present embodiment is dried to moisture by low temperature drying method of the present invention and be14.0%, the Major Nutrient level of the butt fermented feed obtaining is: moisture 14.0%, and metabolizable energy 2047kcal/kg,Protein 18.5%, calcium 0.47%, total phosphorus 0.85%, lysine 0.89%, methionine 0.26%, threonine 0.58%,Tryptophan 0.22%, GABA 0.42g/kg. Enterococcus faecalis is containing viable bacteria 0.8 × 108Cfu/g, total viable count2.9×108cfu/g。
A kind of laying hen premix containing fermented feed providing in the present embodiment, by 1000 parts of gross weights, byFollowing raw material composition: 37 parts of trace element premixing feeds, 4.5 parts of vitamin premixed feeds, vitamin C(90%) 1.5 part, 280 parts of stone flours, 130 parts of calcium monohydrogen phosphates, 2.0 parts of complex enzymes, 2.0 parts of phytases,18 parts of 20 parts of DL-METHIONINEs, 5.0 parts of betaines, 55 parts of salt, Choline Chloride (containing choline 50%),5.0 parts, sodium bicarbonate, 5.0 parts, potassium chloride, 120 parts, butt fermented feed, 0.5 part of ethoxyquinoline, zeolite powder314.5 part.
Wherein, described trace element premixing feed composition: by 1000 parts of gross weights, ferrous sulfate monohydrate145 parts, 20 parts of cupric sulfate pentahydrates, 130 parts of monohydrate zinc sulphates, 140 parts of manganese sulfate monohydrates, a water selenous acid15 parts, sodium, 20 parts of calcium iodates, 5 parts of cobalt chlorides, 0.5 part of ethoxyquinoline, 524.5 parts of zeolite powders.
Wherein, described vitamin premixed feed composition: by 1000 parts of gross weights, retinyl acetate65 parts, vitamin D315 parts, 40 parts of vitamin e1s, 8.0 parts of menadione sodium bisulfites, thiamine hydrochloride7.0 parts of elements, 30 parts, riboflavin, 15 parts of puridoxine hydrochlorides, Cobastab125.0 parts, 40 parts of D-VB5 calcium,90 parts of niacinamide, 3.5 parts, folic acid, 25 parts of Bios, 0.5 part of ethoxyquinoline, powdered rice hulls 556.0Part.
The Major Nutrient level of a kind of 5% laying hen premix containing fermented feed providing in the present embodiment is: waterDivide 8.8%, metabolizable energy 266kcal/kg, protein 4.8%, calcium 13.3%, total phosphorus 2.4%, lysine 0.14%,Methionine 2.02%, threonine 0.10%, tryptophan 0.05%.
The present embodiment provides the laying hen by the above-mentioned preparation of the raw material such as 5% laying hen premix and corn containing fermented feedDaily ration, by gross weight umber 1000, composed as follows: 635 parts of corns (crude protein 8.0%), dregs of beans is (thickProtein 43 .5%) 235 parts, 80 parts of stone flours, containing 50 parts of 5% laying hen premixes of fermented feed.
The Major Nutrient level of the layer diets that the present embodiment is made into is as follows: moisture 11.9%, metabolizable energy 2623Kcal/kg, protein 15.6%, calcium 3.76%, total phosphorus 0.43%, lysine 0.78%, methionine 0.36%,Threonine 0.60%, tryptophan 0.17%.
Embodiment 3
A kind of wet basis state fermented feed that the present embodiment provides, by 1000 parts of gross weights, by following raw material groupBecome:
80 parts of corns, 110 parts, yellow meal worm powder (adult polypide), 90 parts of dregs of beans, 120 parts of Cottonseed Meals, apple60 parts of pomaces, 50 parts of the capsicum dregs of rice, 50 parts of maize peels of whitewashing, 140 parts of wheat brans, 60 parts of bacterium liquid, water240 parts.
In the present embodiment, described bacterium liquid composition: by 1000 parts of gross weights, 500,000,000 cfu/g enterococcus faecalis 8.0Part, 6.0 parts of 30,000,000,000 cfu/g bacillus subtilises and 20,000,000,000 cfu/g bacillus licheniformis (1: 1), 250Hundred million 6.0 parts of cfu/g S. cervisiaes, 40 parts, brown sugar, 940 parts, water.
The Major Nutrient level of above-mentioned wet basis state fermented feed prepared by the present embodiment is: moisture 38.2%, metabolismEnergy 1619kcal/kg, protein 18.6%, calcium 0.57%, total phosphorus 0.70%, lysine 0.96%, methionine0.29%, threonine 0.64%, tryptophan 0.23%, GABA 0.38g/kg, enterococcus faecalis is containing viable bacteria 1.8×108Cfu/g, total viable count 4.5 × 108cfu/g。
Wet basis state fermented feed in the present embodiment is dried to moisture by low temperature drying method of the present invention and be14.0%, the Major Nutrient level of the butt fermented feed obtaining is: moisture 14.0%, and metabolizable energy 2255kcal/kg,Protein 26.0%, calcium 0.79%, total phosphorus 0.97%, lysine 1.47%, methionine 0.41%, threonine 0.89%,Tryptophan 0.32%, GABA 0.42g/kg. Enterococcus faecalis is containing viable bacteria 1.2 × 108Cfu/g, total viable count3.7×108cfu/g。
A kind of laying hen premix containing fermented feed providing in the present embodiment, by 1000 parts of gross weights, byFollowing raw material composition: 43 parts of trace element premixing feeds, 5.5 parts of vitamin premixed feeds, vitamin C(90%) 2.0 part, 260 parts of stone flours, 150 parts of calcium monohydrogen phosphates, 2.5 parts of complex enzymes, 2.8 parts of phytases,22 parts of 22 parts of DL-METHIONINEs, 3.0 parts of betaines, 60 parts of salt, Choline Chloride (containing choline 50%),7.5 parts, sodium bicarbonate, 7.5 parts, potassium chloride, 350 parts, butt fermented feed, 1.0 parts of ethoxyquinolines, zeolite powder61.2 parts.
Wherein, described trace element premixing feed composition: by 1000 parts of gross weights, ferrous sulfate monohydrate190 parts, 40 parts of cupric sulfate pentahydrates, 180 parts of monohydrate zinc sulphates, 180 parts of manganese sulfate monohydrates, a water selenous acid25 parts, sodium, 35 parts of calcium iodates, 20 parts of cobalt chlorides, 1.0 parts of ethoxyquinolines, 329.0 parts of zeolite powders.
Wherein, described vitamin premixed feed composition: by 1000 parts of gross weights, retinyl acetate80 parts, vitamin D325 parts, 90 parts of vitamin e1s, 12 parts of menadione sodium bisulfites, thiamine hydrochloride9.0 parts of elements, 40 parts, riboflavin, 18 parts of puridoxine hydrochlorides, Cobastab127.0 parts, 55 parts of D-VB5 calcium,110 parts of niacinamide, 5.0 parts, folic acid, 35 parts of Bios, 1.0 parts of ethoxyquinolines, powdered rice hulls 413.0Part.
The Major Nutrient level of a kind of 5% laying hen premix containing fermented feed providing in the present embodiment is: waterDivide 11.9%, metabolizable energy 777kcal/kg, protein 10.0%, calcium 13.1%, total phosphorus 2.9%, lysine 0.42%,Methionine 2.30%, threonine 0.25%, tryptophan 0.09%.
The present embodiment provides the laying hen by the above-mentioned preparation of the raw material such as 5% laying hen premix and corn containing fermented feedDaily ration, by gross weight umber 1000, composed as follows: 610 parts of corns (crude protein 8.0%), dregs of beans is (thickProtein 43 .5%) 260 parts, 80 parts of stone flours, containing 50 parts of 5% laying hen premixes of fermented feed.
The Major Nutrient level of the layer diets that the present embodiment is made into is as follows: moisture 12.2%, metabolizable energy 2626Kcal/kg, protein 16.7%, calcium 3.77%, total phosphorus 0.47%, lysine 0.85%, methionine 0.39%,Threonine 0.63%, tryptophan 0.18%.
Feeding experiment
Enter according to People's Republic of China's agricultural industry criteria-" fowl raising standard " (NY/T33～2004)OK, process of the test and result are as follows:
1, test grouping
Select 960 of the blue brown laying hens in sea in 34 week age, be divided into control group A and test by the close principle of laying rateGroup B, C, tetra-processed group of D, 6 repetitions of every processing, 40 of every repetitions, every group totally 240, testBefore beginning, carry out band chicken sterilization in hen house, 4 weeks experimental periods.
2, test daily ration
Test daily ration is the adequate diet being mixed with by corn, dregs of beans, stone flour and 5% laying hen premix, each formerThe ratio of material is identical in A, B, C, tetra-processed group daily rations of D. 5% laying hen in control group A daily ration is pre-Batch mixing is not containing fermented feed; Experiment group B adopts the daily ration of recommending in the embodiment of the present invention 1, wherein gamma-aminoButyric acid content is 5.5g/t, and 5% premix is the 5% laying hen premix containing fermented feed, and A, two groups of B are usedNutritional labeling in two kinds of premixes is identical; Test group C adds the micro-ecological system of 400g/t in basal dietAgent, this probiotics comprises that 200g/t's (can not secrete gamma-amino containing viable bacteria 500,000,000 cfu/g Bacillus acidi lacticisButyric acid), 150g/t by containing viable bacteria 20,000,000,000 cfu/g bacillus subtilises and 20,000,000,000 cfu/g lichens gemma barsBacterium by compound Bacillus, the 50g/t of preparation in 1: 1 containing viable bacteria 200,000,000 cfu/g S. cervisiaes; Test group DThe synthetic GABA 5.5g/t of the chemical industry of contained equivalent in interpolation and experiment group B in basal diet. TestDiet Formula and trophic component are respectively in table 1,2.
Table 1 laying ration composition
| Raw material | A | B | C | D |
| Corn, 8%CP | 620 | 560 | 620 | 620 |
| Dregs of beans, 43.5%CP | 250 | 210 | 250 | 250 |
| Stone flour, 37%Ca | 80 | 80 | 80 | 80 |
| 5% laying hen premix | 50 | - | 50 | 50 |
| Containing 5% laying hen premix of fermented feed | - | 50 | - | - |
| Probiotics | - | - | 0.4 | - |
| GABA | - | - | - | 0.0055 |
| Add up to | 1000 | 1000 | 1000.4 | 1000.0055 |
Table 2 laying ration main nutrient composition and level
| Nutritive index | A | B | C | D |
| Metabolizable energy, kcal/kg | 2633 | 2631 | 2630 | 2631 |
| Thick protein, % | 16.3 | 16.3 | 16.3 | 16.3 |
| Calcium, % | 3.76 | 3.76 | 3.76 | 3.76 |
| Total phosphorus, % | 0.43 | 0.43 | 0.43 | 0.43 |
| Lysine, % | 0.83 | 0.83 | 0.83 | 0.83 |
| Methionine, % | 0.38 | 0.38 | 0.38 | 0.38 |
| Threonine, % | 0.62 | 0.62 | 0.62 | 0.62 |
3, feeding and management
Test chicken adopts three and half stageds to raise in cages, and every cage 2-3, day feeding 2 times. Each processed group is with heavyBe unit again, alternately, the error causing to reduce environmental condition. Laying hen free choice feeding, freely drinks water,Illumination 16h/d. Carry out ventilation in daily house, regularly clear excrement, routine immunization. Record hen house every dayAmount of drinking water, illumination, temperature, humidity ight soil outward appearance, chicken group health, medicine for treatment and extremely naughty situation.
4, index detects
(1) egg laying performance and Egg Quality: respectively observe to off-test every day at the upper and lower noon from on-testAnd pick up egg once, and taking repeating groups as unit record feed intake, egg number, weigh egg size, calculate each group of laying henAverage egg production, average egg weight, average daily ingestion amount, feedstuff-egg ratio, spot egg rate, rate of broken eggs.
Average egg production (%)=total egg number/(chicken number × number of days) × 100
Average egg weight (g/)=total egg size/(chicken number × number of days)
Average daily ingestion amount (g/ only)=total feed intake/(chicken number × number of days)
Feedstuff-egg ratio=average daily ingestion amount/average day egg production
Spot egg rate (%)=spot egg number/total egg number × 100
Rate of broken eggs (%)=broken egg number/total egg number × 100
(2) mensuration of antibody level of serum: off-test that morning 8:00, laying hen under empty stomach state,From wing vein blood sample collection, every repeating groups is got 3 chickens, and every chicken gathers 2ml, leaves standstill 3000 at 4 DEG CCentrifugal 10 minutes of r/min, draws supernatant and is prepared into serum, in-20 DEG C of Refrigerator stores. According to " high causing a diseaseProperty avian influenza prevention technical specification " method of regulation, blood serum sample is carried out to blood clotting suppresses titration, surveysGo out tiring of IgA, IgG, IgM.
5, data processing
After test data is compiled, adopt SPSS10.0 statistical software to data carry out variance analysis andDuncan ' s multiple ratio. The significance level of difference is set: P < 0.05 is significant difference, and P < 0.01 is for poorHeteropole is remarkable.
6, result
(1) egg laying performance of laying hen: A, B, C, tetra-processed group laying hens of D are tested laying eggs of 4 time-of-weeksPerformance is in table 3.
The impact of the daily ration that table 3 is prepared containing the laying hen premix of fermented feed on Layer Production Performance
Note: 1, above data are the mean value of each processed group.
2, colleague's data shoulder motes same letter represents not remarkable (P > 0.05) of difference, and the different lowercase alphabets of shoulder motes show significant difference (P < 0.05);Extremely significantly (P < 0.01) of different uppercase expression difference, lower same.
The average egg production result of different disposal group shows: test 1-2 week, 3-4 week, and all with B group laying rateThe highest; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D laying rate improve respectively 1.72%(P < 0.05), 0.50% (P > 0.05), 0.11% (P > 0.05); Experiment group B improves 1.61% compared with D laying rate(P < 0.05). Show, experiment group B laying rate is all significantly increased compared with control group and test group D, and triesTesting group C, D laying rate improves not remarkable compared with control group.
The egg size result of different disposal group shows: only, in test 3-4 week, experiment group B is compared with control group A egg sizeSignificantly improve 1.02% (P < 0.05); And other each phase, between each group, egg size is showed no significant difference (P > 0.05).
The feed intake result of different disposal group shows:: test 1-2 week, 3-4 week, thorough phase of 1-4, control groupBetween each group of A and experiment group B, C, D, feed intake is all less than significantly different (P > 0.05).
The feedstuff-egg ratio result of different disposal group shows: test 1-2 week, 3-4 week, all minimum with B group feedstuff-egg ratio;Compared with control group A, the 4 thorough phases of test, experiment group B, C, D feedstuff-egg ratio reduce respectively 3.72% (P <0.05), 1.42% (P > 0.05), 1.08% (P > 0.05); Experiment group B is compared with the remarkable reduction by 2.67% of C feedstuff-egg ratio(P < 0.05), between test group C, D feedstuff-egg ratio without remarkable difference, compared with control group A also without significantly improving (P ＞0.05)。
Result shows, 5% laying hen premix containing fermented feed of a kind of in the embodiment of the present invention of feeding 1 andThe daily ration of preparation, the improvement of laying rate of laying hen, feedstuff-egg ratio is the most obvious, is significantly better than control group A, material eggThan being significantly better than the test group D that adds GABA. This shows, in 5% laying hen premix, and vast scaleAdd butt state fermented feed, microecological regulation and control effect and the gamma-amino of stack performance viable bacteria and metaboliteThe central neurotransmitter regulating and controlling effect of butyric acid, is significantly improved to Layer Production Performance; And add with routine doseThe probiotics adding and GABA be not to there is no remarkable shadow in the egg laying performance of egg-laying peak laying henRing.
(2) Egg Quality: A, B, C, tetra-processed group laying hens of D are tested spot egg rate and the broken egg of 4 time-of-weeksRate is in table 4.
The affect unit of the daily ration that table 4 is prepared containing the laying hen premix of fermented feed on Egg Quality: %
The rate of broken eggs result of different disposal group shows: test 1-2 week, 3-4 week, all organize the broken egg of egg with BRate is minimum; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D rate of broken eggs reduce respectively 20.41%(P < 0.05), 7.65% (P > 0.05), 8.16% (P > 0.05); Rate of broken eggs between experiment group B, C and DWithout significant difference (P > 0.05).
The spot egg rate result of different disposal group shows: test 1-2 week, 3-4 week, all organize the spot egg of egg with BRate is minimum; Compared with control group A, the 4 thorough phases of test, experiment group B, C, D spot egg rate reduce respectively 19.77%(P < 0.05), 5.39% (P > 0.05), 5.49% (P > 0.05); Spot egg rate between experiment group B, C and DWithout significant difference (P > 0.05).
Result shows, the daily ration of 5% laying hen premix preparation of the vast scale of feeding interpolation butt fermented feed is foldedAdd performance viable bacteria and the microecological regulation and control effect of metabolite and the central neurotransmitter of GABA regulation and control workWith, effectively promote calcium, phosphorus absorption and eggshell calcium, phosphorus and manganese etc. trace element deposition, reduce rate of broken eggsWith spot egg rate, and the probiotics adding with routine dose and GABA are in egg-laying peakThe Egg Quality of laying hen is not significantly improved.
(3) antibody horizontal: A, B, C, tetra-processed group laying hens of D are the serum antibody water after 4 weeks in testFlat in table 5.
The impact of the daily ration that table 5 is prepared containing the laying hen premix of fermented feed on laying hen antibody horizontal
| Index | A | B | C | D |
| IgA | 0.0168a | 0.0213b | 0.0187ab | 0.0181ab |
| IgG | 0.0922 | 0.1014 | 0.0956 | 0.0939 |
| IgM | 0.0113 | 0.0135 | 0.0118 | 0.0120 |
The IgA antibody horizontal result of different disposal group shows: tested for 4 weekends, serum IgA content is with testB is the highest for group; Compared with control group A, experiment group B, C, D serum IgA improve respectively 26.79% (P <0.05), 11.31% (P > 0.05), 7.74% (P > 0.05); Serum IgA content between experiment group B, C, DNot significantly different (P > 0.05).
The IgG antibody horizontal result of different disposal group shows: tested for 4 weekends, serum IgG content is with testB is the highest for group; Compared with control group A, experiment group B, C, D serum IgG improve respectively 9.98%, 3.69%,1.84%, but all without significant difference (P > 0.05).
The IgM antibody horizontal result of different disposal group shows: tested for 4 weekends, serum IgM content is with testB is the highest for group; Compared with control group A, experiment group B, C, D serum IgM improve respectively 19.47%, 4.42%,6.19%, but all without significant difference (P > 0.05).
Result shows, laying hen premix containing fermented feed of a kind of in the embodiment of the present invention of feeding 1 and joiningThe daily ration of system, the control group that Laying-hen Serum IgA antibody horizontal does not add fermented feed significantly improves. This tableBright, in 5% laying hen premix, vast scale adds butt state fermented feed, and stack performance viable bacteria and metabolism are producedThe microecological regulation and control effect of thing and the central neurotransmitter regulating and controlling effect of GABA, anti-to Laying-hen SerumThe body level effect that is significantly increased; And the probiotics adding with routine dose and GABA are to locatingAntibody horizontal in egg-laying peak laying hen all has no significant effect.
Finally it should be noted that: above embodiment is only also unrestricted described in the invention in order to the present invention to be describedTechnical scheme; Therefore, although this description has carried out the present invention with reference to each above-mentioned embodiment in detailThin explanation, still, those of ordinary skill in the art should be appreciated that still and can repair the present inventionChange or be equal to replacement; And all do not depart from technical scheme and the improvement thereof of the spirit and scope of the present invention, it is equalShould be encompassed in claim scope of the present invention.
Claims (10)
1. containing a 5% laying hen premix of fermented feed, it is characterized in that, by 1000 parts of gross weights,Comprise: 30～50 parts of trace element premixing feeds, 4.5～5.5 parts of vitamin premixed feeds, vitaminC1.0～3.0 part, 230～320 parts of stone flours, 120～180 parts of calcium monohydrogen phosphates, 2.0～3.0 parts of complex enzymes, plant2.0～3.0 parts of enzymes of acid, 15～30 parts of DL-METHIONINEs, 2.0～5.0 parts of betaines, 50～70 parts of salt,15～25 parts of Choline Chlorides (containing choline 50%), 5.0～20 parts, sodium bicarbonate, 5.0～20 parts, potassium chloride, dry150～400 parts, base fermented feed, 0.5～1.5 part of ethoxyquinoline, 0～400 part of zeolite powder.
2. the 5% laying hen premix containing fermented feed according to claim 1, is characterized in that, by total1000 parts of meters of weight, the consisting of of described trace element premixing feed: 140～200 parts of ferrous sulfate monohydrates,140～200 parts of 15～60 parts of cupric sulfate pentahydrates, 120～200 parts of monohydrate zinc sulphates, manganese sulfate monohydrate, one15～25 parts of water sodium selenites, 15～40 parts of calcium iodates, 5.0～40 parts of cobalt chlorides, ethoxyquinoline 0.5～233.5～549.5 parts of 1.5 parts, zeolite powder.
3. the 5% laying hen premix containing fermented feed according to claim 1, is characterized in that, by total1000 parts of meters of weight, the consisting of of described vitamin premixed feed: 50～80 parts of retinyl acetates,Vitamin D315～30 parts, 20～200 parts of vitamin e1s, 5.0～15 parts of menadione sodium bisulfites, salt6.0～10 parts of allithiamine elements, 5～45 parts of Riboflavin Tetrabutyrates, 13～20 parts of puridoxine hydrochlorides, Cobastab124.0～8.0 parts, 35～60 parts of D-VB5 calcium, 90～125 parts of niacinamide, 3.5～6.0 parts, folic acid, Bio 25～40 parts, 0.5～1.5 part of ethoxyquinoline, 359.5～608.0 parts of powdered rice hulls.
4. the 5% laying hen premix containing fermented feed according to claim 1, is characterized in that, by total1000 parts of meters of weight, the consisting of of described complex enzyme: 700 parts of zytases, 100 parts of cellulases, β-50 parts of dextranases, 50 parts of pectases, 100 parts of maize cob meals.
5. the 5% laying hen premix containing fermented feed according to claim 1, is characterized in that, described inButt fermented feed is prepared through low temperature drying by wet basis state fermented feed,
And by 1000 parts of gross weights, described wet basis state fermented feed consists of: 30～100 parts of corns, HuangMealworm powder 50-120 part, 0～150 part of dregs of beans, 50～150 parts of Cottonseed Meals, 40～120 parts of pomaces, peppery50～100 parts of the green pepper dregs of rice, 40～120 parts of maize peels of whitewashing, 100～300 parts of wheat brans, bacterium liquid 40～60200～300 parts, part, water.
6. the 5% laying hen premix containing fermented feed according to claim 5, is characterized in that, by total1000 parts of meters of weight, the consisting of of described bacterium liquid: enterococcus faecalis F6 (EnterococcusfaeciumF6) 5～2.5～7.5 parts, mixture, the S. cervisiae 2.5～7.5 of 15 parts, bacillus subtilis and bacillus licheniformisPart, 20～50 parts, brown sugar, 920～970 parts, water.
7. the 5% laying hen premix containing fermented feed according to claim 6, is characterized in that, described inThe ratio of bacillus subtilis and bacillus licheniformis in the mixture of bacillus subtilis and bacillus licheniformisIt is 1: 1.
8. the 5% laying hen premix containing fermented feed according to claim 5, is characterized in that, will wetGround state fermented feed low temperature drying is prepared the method for butt fermented feed: wet basis state fermented feed feeds through hopperFlash dryer; Steam heater produces hot-air, and temperature is no more than 100 DEG C; Carry out hot-air seasoning; ControlDischarging opening hot blast temperature is no more than 80 DEG C, obtains butt fermented feed;
The preparation method of wherein said wet basis state fermented feed: impurity removing, sieve with 1.5～2.8mm after weighingPulverize in hole; Raw material after pulverizing is carried out to mixed once 2～5 minutes; Bacterial classification activates in 30～45 DEG C of water temperatures0.5～1.0h; Bacterium liquid after activation be pulverized and mixed after raw material mix again; Packed solid under 20～45 DEG C of conditionsState anaerobic fermentation 5～10 days.
9. the 5% laying hen premix containing fermented feed according to claim 8, is characterized in that, described inThe Major Nutrient level of butt fermented feed is: moisture 13～16%, and metabolizable energy 1900～2450kcal/kg,Protein 16～30%, calcium 0.35～0.90%, total phosphorus 0.60～0.95%, lysine 0.70～1.55%, eggPropylhomoserin 0.20～0.55%, threonine 0.40～0.95%, tryptophan 0.15～0.40%, GABA 0.22～0.50g/kg, enterococcus faecalis is containing viable bacteria 0.7 × 108～1.7×108Cfu/g, total viable count 2.0 × 108～6.0×108cfu/g。
10. by the laying hen adequate diet being mixed with containing 5% laying hen premix of fermented feed, be characterised in that:By 1000 parts of gross weights, comprising: 580～660 parts of corns, 220～280 parts of dregs of beans, 80 parts of stone flours,And claim 1 is to the 5% laying hen premix 50 containing fermented feed of arbitrary claim in claim 9Part.
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| CN111345396A (en) * | 2018-12-24 | 2020-06-30 | 中粮生物化学（安徽）股份有限公司 | Fermented feed, preparation method and application thereof, and laying hen feed |
| CN111134243A (en) * | 2020-01-20 | 2020-05-12 | 山东众成饲料科技有限公司 | Conservation piglet feed for producing pork without antibiotics and preparation method |
| CN112314801A (en) * | 2020-10-25 | 2021-02-05 | 阿克苏泰昆饲料有限责任公司 | Corn byproduct fermented feed for laying hens and preparation and application thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| CN114601022A (en) * | 2022-03-15 | 2022-06-10 | 天津博菲德科技有限公司 | Preparation method of fermented chili meal and application of fermented chili meal in laying hen feed |
| CN114601074A (en) * | 2022-04-09 | 2022-06-10 | 河南宏展生物科技有限公司 | Fermented feed and compound feed for laying hens and preparation method of fermented feed and compound feed |
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