CN105560373A - New use of traditional Chinese medicine Clausena excavata Burm. in preparation of medicines - Google Patents
New use of traditional Chinese medicine Clausena excavata Burm. in preparation of medicines Download PDFInfo
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- CN105560373A CN105560373A CN201511022108.1A CN201511022108A CN105560373A CN 105560373 A CN105560373 A CN 105560373A CN 201511022108 A CN201511022108 A CN 201511022108A CN 105560373 A CN105560373 A CN 105560373A
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- 235000017361 Clausena excavata Nutrition 0.000 title claims abstract description 72
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 229940079593 drug Drugs 0.000 title abstract description 5
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- 239000002778 food additive Substances 0.000 claims abstract description 12
- 201000010099 disease Diseases 0.000 claims abstract description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 11
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- 239000000341 volatile oil Substances 0.000 claims description 25
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- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Dispersion Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a use of a traditional Chinese medicine Clausena excavata Burm. in preparation of medicines for treating or preventing fungus induced diseases. Researches confirm that the traditional Chinese medicine Clausena excavata Burm. can effectively kill or inhibit Asperigillus flavus and other Aspergillus fungi, so the traditional Chinese medicine Clausena excavata Burm. can be used to treat or prevent diseases induced by infection of Asperigillus flavus and other Aspergillus fungi. The traditional Chinese medicine Clausena excavata Burm. can be used for a long term without side effects, so the biological safety of the traditional Chinese medicine Clausena excavata Burm. is guaranteed, and the traditional Chinese medicine Clausena excavata Burm. can be used as a medicine, and also can be used as a food additive.
Description
Technical field
The present invention relates to field of Chinese herbal medicine technology, particularly relate to Chinese medicine Clausena excavata Burm.f. and preparing the novelty teabag in medicine.
Background technology
Chinese medicine Clausena excavata Burm.f., has another name called wood pulp daughter root, MANSHANXIANG, clear Radix Solani Melongenae, Qiang Jiang, cubeb litsen tree, is the root of canella cubeb litsen tree and aerial parts and mature seed.Growth on small stream limit, hillside fields or weeds border, be distributed in south China each province of China, medicine source is very abundant.
Some books of Chinese medicine Clausena excavata Burm.f. are also on the books, but the effect illustrated is spleen invigorating, dampness, regulating QI, helps digestion, and treat cold syndrome of the stomach, dyspepsia, have loose bowels.Not yet disclose the medical usage of the disease of the Aspergillus flavus of antifungal especially in Eurotium and treatment fungal infection.
Aspergillus infection can give rise to diseases, and comprises cancer and dermatosis etc.Aspergillosis is conditioned pathogen, main air-borne transmission, after the conidium produced enters upper respiratory tract, can long-term adhesion and parasitizing on the mucosa of nasal cavity, nasopharynx and pars oralis pharyngis, and do not cause any symptom.When there is chronic inflammatory disease nasal sinuses local, wound, when hole intracavity has pathologic ischesis or intranasal ventilation drain to be obstructed, just various aspergillosis can be there is.Meanwhile, can be parasitic along air flue after the conidium suction of aspergillosis, and can alveolar be invaded, form various pulmonary aspergillosis.
As everyone knows, aflatoxin (aflatoxinB1, AFB1) on epidemiology, substantial connection is had with hepatocarcinoma, in China southeastern coastal areas, warmer climate is moist, and the growth of suitable Aspergillus flavus, in corn and beans, the pollution of flavacin is comparatively general, and these areas are also the districts occurred frequently of hepatocarcinoma.Research shows, the intake of AFB1 and the mortality rate of hepatocarcinoma are proportionate.Up to now, AFB1 is known as the strongest carcinogen of nature.
In addition, dermatophytosis especially tinea manuum and tinea pedis is closely related relevant to fungal infection, wherein relates to multiple fungus.Research shows, dermatophytosis is except relevant to trichophyton and Epidermophyton, also relevant to non-skin tinea bacterium such as Aspergillus flavus.
Summary of the invention
In life for many years and clinical practice, the present inventor finds, every Chinese medicine Clausena excavata Burm.f. is as the area of the storage keeping underbed of corn, and onset of liver cancer rate is relatively little, treatment adjuvant using the extract of Clausena excavata Burm.f. as hepatocarcinoma patient, can extend the life cycle of patient.
Therefore, first object of the present invention is, discloses the effect of the aspergillus flavus-resistance mycete of Chinese medicine Clausena excavata Burm.f..
Another object of the present invention is, discloses the antifungal ability of Chinese medicine Clausena excavata Burm.f..
The invention provides a kind of Chinese medicine Clausena excavata Burm.f. and preparing the novelty teabag in medicine, be achieved through the following technical solutions:
Chinese medicine Clausena excavata Burm.f. is in preparation treatment or prevent by the purposes in the medicine of fungus-caused disease.
As preferred version of the present invention, above-mentioned fungus is Eurotium, is preferably Aspergillus flavus.
As preferred version of the present invention, above-mentioned disease is hepatocarcinoma or dermatophytosis, and this dermatophytosis is preferably tinea manuum and tinea pedis.
As preferred version of the present invention, said medicine is the medicine containing Chinese medicine Clausena excavata Burm.f. extract.
As preferred version of the present invention, above-mentioned Chinese medicine Clausena excavata Burm.f. extract is Chinese medicine Clausena excavata Burm.f. volatile oil.
Scheme as a further improvement on the present invention, above-mentioned Chinese medicine Clausena excavata Burm.f. volatile oil is greater than 1:600 with the cumulative volume ratio of said medicine.
As further improved plan of the present invention, the volumn concentration of above-mentioned Chinese medicine Clausena excavata Burm.f. volatile oil in said medicine is greater than 1%.
The present invention also provides the novelty teabag of a kind of Chinese medicine Clausena excavata Burm.f. in preparation food additive, is achieved through the following technical solutions:
The purposes of Chinese medicine Clausena excavata Burm.f. in preparation food additive, this food additive can be killed or Antifungi.
Scheme as a further improvement on the present invention, it is obtained that above-mentioned food additive is that Chinese medicine Clausena excavata Burm.f. volatile oil adds in edible oil.
As further improved plan of the present invention, above-mentioned Chinese medicine Clausena excavata Burm.f. volatile oil is greater than 1:500 according to volume ratio and adds in edible oil.
Inventor confirms after deliberation, and the fungus of the Eurotiums such as Aspergillus flavus can effectively be killed or suppress to Chinese medicine Clausena excavata Burm.f., therefore may be used for the disease caused with fungal infection such as Aspergillus flavus treatment or among preventing.In addition, because Chinese medicine Clausena excavata Burm.f. uses for a long time in Chinese medicine, no side effects, therefore biological safety is guaranteed, can as medicine, also can as food additive.
Accompanying drawing explanation
Fig. 1 is that the fungistatic effect after formula A cultivates 3 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is negative control, and right figure is formula A;
Fig. 2 is that the fungistatic effect after formula B cultivates 3 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is negative control, and right figure is formula B;
Fig. 3 is that the fungistatic effect after formula C cultivates 3 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is negative control, and right figure is formula C;
Fig. 4 is that the fungistatic effect after formula A, B and C cultivate 3 days during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein upper figure is formula A, and lower-left figure is formula B, and bottom-right graph is formula C;
Fig. 5 is that the fungistatic effect after formula A cultivates 6 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is formula A, and right figure is negative control;
Fig. 6 is that the fungistatic effect after formula B cultivates 6 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is formula B, and right figure is negative control;
Fig. 7 is that the fungistatic effect after formula C cultivates 7 days with negative control during Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus to be tested compares, and wherein left figure is formula C, and right figure is negative control.
Detailed description of the invention
By reference to the accompanying drawings the present invention is described in further detail below by detailed description of the invention.
The novelty teabag of the suppression Aspergillus flavus of Chinese medicine Clausena excavata Burm.f. is confirmed below by experimental data.
Chinese medicine Clausena excavata Burm.f. extract suppresses Aspergillus flavus
1. materials and methods
Bacterial strain: Aspergillus flavus
Reagent: Chinese medicine Clausena excavata Burm.f. volatile oil is drawn stock solution (get Clausena excavata Burm.f. aerial parts, extract its volatile oil with volatile oil extractor, yield 0.9%, method is shown in 2005 editions Chinese Pharmacopoeias one).Get its 750 μ l stock solution+4%Triton (spreader) (formula A) respectively, 150 μ l stock solution+600 μ l sterilized water+4%Triton (formula B), 75 μ l stock solution+675 μ l sterilized water+4%Triton (formula C), 750 μ l sterilized water+4%Triton (negative control).
Culture medium: PDA culture medium
Cultural method: respectively get formula A, formula B, formula C and negative control 250 μ l and join in the PDA culture medium that 15ml melted respectively, mixing a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, gets a fritter by the Aspergillus flavus culture activated and is inoculated on flat board, arrange three repetitions, cultivate after 3 days and 6 days, observe and take a picture.After formula A adds, it is 60 that its final concentration is equivalent to mother solution
-1; After formula B adds, it is 300 that its final concentration is equivalent to mother solution
-1; After formula C adds, it is 600 that its final concentration is equivalent to mother solution
-1; After negative control adds, be equivalent to not add Chinese medicine extract.
2. results and analysis
Cultivate after 3 days, except negative control, variable concentrations Chinese medicine extract is all inhibited to Aspergillus flavus growth.
Concrete reference diagram 1-4, wherein figure 1 illustrate formula A cultivate 3 days with negative control after fungistatic effect compare, wherein left figure is negative control, and right figure is formula A; Visible negative control has a large amount of Aspergillus flavus colony growth, and formula A only has indivedual Aspergillus flavus colony growth.
Fig. 2 show formula B cultivate 3 days with negative control after fungistatic effect compare, wherein left figure is negative control, and right figure is formula B; Visible negative control has a large amount of Aspergillus flavus colony growth, and formula B only has indivedual Aspergillus flavus colony growth.
Fig. 3 show formula C cultivate 3 days with negative control after fungistatic effect compare, wherein left figure is negative control, and right figure is formula C; Visible negative control has a large amount of Aspergillus flavus colony growth, and formula C only has indivedual Aspergillus flavus colony growth.
Fig. 4 show formula A, B and C cultivate 3 days after fungistatic effect compare, wherein upper figure is formula A, and lower-left figure is formula B, and bottom-right graph is formula C; Visible fungistatic effect is relevant to the concentration of the Chinese medicine Clausena excavata Burm.f. volatile oil in formula, and the concentration of Chinese medicine Clausena excavata Burm.f. volatile oil is higher, and fungistatic effect is better.
Cultivate after 6 days, its result is consistent with the fungistatic effect cultivated 3 days.
Concrete reference diagram 5-7, wherein figure 5 show formula A cultivate 6 days with negative control after fungistatic effect compare, wherein left figure is formula A, and right figure is negative control; Visible formula A only has individual colonies to grow, and negative control has a large amount of colony growth, and is almost full of culture dish.
Fig. 6 show formula B cultivate 6 days with negative control after fungistatic effect compare, wherein left figure is formula B, and right figure is negative control; Visible formula B only has individual colonies to grow, and negative control has a large amount of colony growth, and is almost full of culture dish.
Fig. 7 show formula C cultivate 7 days with negative control after fungistatic effect compare, wherein left figure is formula C, and right figure is negative control; Visible formula C only has individual colonies to grow, and negative control has a large amount of colony growth, and is almost full of culture dish.
In conjunction with first time experiment, after Clausena excavata Burm.f. volatile oil final concentration is diluted 1000 times by us, find that it suppresses Aspergillus flavus effect more weak, even substantially do not have and suppress Aspergillus flavus effect, the fungistatic effect of visible Clausena excavata Burm.f. volatile oil is relevant to concentration.
In addition, experiment shows: get Clausena excavata Burm.f. decocting liquid and repeat above-mentioned experiment, finds that it does not have and suppresses Aspergillus flavus effect.
In sum, the liposoluble constituent volatile oil of this Chinese medicine is the effective ingredient suppressing Aspergillus flavus, and extractive of volatile oil is when being diluted to 600 times, and it is still inhibited to Aspergillus flavus.
Consider that Aspergillus flavus is the Typical Representative of Eurotium, and the general character of various bacterium in pathogenic in Eurotium, we think that Chinese medicine Clausena excavata Burm.f. suppresses to comprise the multiple fungus of Eurotium widely, thus by using Chinese medicine Clausena excavata Burm.f., the disease relevant to fungal infection can be treated.
Based on discovery of the present invention, we have proposed Chinese medicine Clausena excavata Burm.f. and treat in preparation or prevent by the purposes in the medicine of fungus-caused disease.Wherein, fungus is Eurotium especially, more preferably Aspergillus flavus.
Consider and confirm that Aspergillus flavus is relevant to hepatocarcinoma or dermatophytosis (such as tinea manuum and tinea pedis), the medicine therefore adopting Chinese medicine Clausena excavata Burm.f. to make in the present invention can be used in Hepatoma therapy or dermatophytosis.
Medicine of the present invention can be the medicine containing Chinese medicine Clausena excavata Burm.f. extract, particularly Chinese medicine Clausena excavata Burm.f. volatile oil, and its preparation method is well known in the art, the method such as, mentioned in 2005 editions Chinese Pharmacopoeias one.Certainly, the present invention does not get rid of Chinese medicine Clausena excavata Burm.f. volatile oil prepared by other method for the preparation of medicine of the present invention yet.
In the present invention, alleged " medicine " can refer to Chinese medicine Clausena excavata Burm.f. extract itself, particularly Chinese medicine Clausena excavata Burm.f. volatile oil itself, also can be grouped into pharmaceutical composition containing other one-tenth, other composition comprises: substrate (base), carrier, additive (such as excipient, binding agent, disintegrating agent, lubricant, solvent, sweetener, coloring agent, correctives, odor masking agent, surfactant, humidizer, antiseptic, pH adjusting agent and thickening agent) etc.These compositions are mentioned in such as " Dictionary of Chinese Pharmacy ".
The dosage form of medicine, as tablet, coated tablet, powder, granule, granula subtilis, capsule, pill, liquid agent, suspensoid, Emulsion, gel, masticatory, film agent etc.Oral formulations, such as tablet, capsule, granule, powder, syrup etc.; Non-oral formulation, such as suppository, inhalant, transdermic absorbent, skin preparations for extenal use, eye drop, nasal drop etc.
Dosage and administration time, be not particularly limited, and can be that the seriousness according to patient age, patients symptomatic, other conditions etc. are suitably selected.
" medicine " alleged by the present invention also can comprise " food additive ", acceptable substrate, carrier and food additive on food hygiene can also be comprised, such as soy sauce, monosodium glutamate and flavoring agent etc., these food additive can add when preparing food, also can immediately add before consumption.
In fact, oil preparation prepared by employing Clausena excavata Burm.f. is as flavorant or flavoring agent, and among the people also have use.When its concentration is suitable for, its fragrance is pure can people, has similar Rhizoma Zingiberis Recens to steep the abnormal smells from the patient of Semen Sojae Preparatum, is also called Radix Litseae among the people.Be before this and do not know that it has the effect suppressing Aspergillus flavus.Inventor finds, uses Clausena excavata Burm.f. oil preparation to add in edible oil by 1:500, after 3 hours, can suppress the Aspergillus flavus in this food.The volatilization ball using Clausena excavata Burm.f. oil preparation to make hangs in the container of meter bean, after 3 hours, in this container edifying to rice bean in Aspergillus flavus be killed.Certainly, Clausena excavata Burm.f. oil preparation is added during processing oil rice even more ideal.
In a preferred embodiment of the invention, Chinese medicine Clausena excavata Burm.f. volatile oil is greater than 1:600 with the cumulative volume ratio of medicine or food.More preferably, the volumn concentration of Chinese medicine Clausena excavata Burm.f. volatile oil in medicine is greater than 1%.Show after deliberation, the Emulsion of 1% made by volatile oil prepared by application Clausena excavata Burm.f., can treat tinea manuum and tinea pedis.
In fact, the oil preparation that prepared by Clausena excavata Burm.f. has strong inhibitory action to various fungus.Therefore, the Emulsion that application Clausena excavata Burm.f. oil preparation is made has special efficacy to tinea manus and pedis.At present, in clinical treatment, to the treatment use griseofulvin, ketoconazole, two property mycin B etc. of tinea manus and pedis, these medicines have obvious toxic and side effects to liver, and its medical expense is expensive especially.Medicine of the present invention is used for the treatment of tinea manus and pedis, and not only expense is cheap, and uses as reliable Chinese herbal medicine due to Clausena excavata Burm.f. always, and its side effect is less.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. Chinese medicine Clausena excavata Burm.f. is treated in preparation or is prevented by the purposes in the medicine of fungus-caused disease.
2. purposes according to claim 1, is characterized in that, described fungus is Eurotium, is preferably Aspergillus flavus.
3. purposes according to claim 1, is characterized in that, described disease is hepatocarcinoma or dermatophytosis, and described dermatophytosis is preferably tinea manuum and tinea pedis.
4. the purposes according to any one of claim 1-3, is characterized in that, described medicine is the medicine containing Chinese medicine Clausena excavata Burm.f. extract.
5. purposes according to claim 4, is characterized in that, described Chinese medicine Clausena excavata Burm.f. extract is Chinese medicine Clausena excavata Burm.f. volatile oil.
6. purposes according to claim 5, is characterized in that, described Chinese medicine Clausena excavata Burm.f. volatile oil is greater than 1:600 with the cumulative volume ratio of described medicine.
7. purposes according to claim 6, is characterized in that, the volumn concentration of described Chinese medicine Clausena excavata Burm.f. volatile oil in described medicine is greater than 1%.
8. the purposes of Chinese medicine Clausena excavata Burm.f. in preparation food additive, this food additive can be killed or Antifungi.
9. purposes according to claim 8, is characterized in that, it is obtained that described food additive is that Chinese medicine Clausena excavata Burm.f. volatile oil adds in edible oil.
10. purposes according to claim 9, is characterized in that, described Chinese medicine Clausena excavata Burm.f. volatile oil is greater than 1:500 according to volume ratio and adds in edible oil.
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| CN112715797A (en) * | 2019-10-11 | 2021-04-30 | 姜桂良 | Preparation method of compound active bacterium beverage |
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Application publication date: 20160511 |