CN105557545A - Learning and memorizing training system for odor substance - Google Patents

Learning and memorizing training system for odor substance Download PDF

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Publication number
CN105557545A
CN105557545A CN201610118822.9A CN201610118822A CN105557545A CN 105557545 A CN105557545 A CN 105557545A CN 201610118822 A CN201610118822 A CN 201610118822A CN 105557545 A CN105557545 A CN 105557545A
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time
relay coil
control circuit
delay relay
air
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CN105557545B (en
Inventor
朱启文
赵建文
匡宝平
郭鹏骥
李典典
李晓秀
崔跃
刘殊余
刘慧颖
金戈
邹丹
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Shenyang Medical College
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Shenyang Medical College
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K15/00Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
    • A01K15/02Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
    • A01K15/027Exercising equipment, e.g. tread mills, carousels

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)

Abstract

A learning and memorizing training system for an odor substance comprises a box, an odor device, an air exchanging system, an electrical stimulation device and a control circuit. The learning and memorizing training system is characterized in that an air inlet and air outlets are arranged on the box, the air inlet and the air outlets are connected with the air exchanging system, and the odor device comprises an odor channel and an air source, wherein the odor channel and the air inlet in the box are arranged correspondingly, and the air source is communicated with the air inlet end of the odor channel; the air exchanging system comprises an air cylinder and an exhaust fan; the electrical stimulation device comprises a grid plate formed by positive and negative electrodes arranged alternately, wherein the grid plate is limited inside the box; a food rewarding device is installed on the grid plate, a hiding table located on the inner wall of the box is installed above the grid plate through a supporting spring, and a supporting air cylinder capable of adjusting the vertical or horizontal state of the hiding table is arranged at the bottom of the hiding table. Through negative electrical stimulation or food and drinking water positive inducing training, a mouse generates a behavior response for a kind of odor to serve as an observation index for recognizing the substance, and whether a target search object exists nearby or not is determined.

Description

The learning and memory training system of odoring substance
Technical field
The present invention relates to training or the exercise device of a kind of animal, particularly relate to a kind of learning and memory training system of odoring substance, it is mainly applicable to the training of experimental mouse such as drug law enforcement mouse, explosion-proof mouse etc.
Background technology
It is investigated, at home and abroad, to enforce the drug law multiplex drug detector dog with animal, by mouse drug law enforcement existing precedent abroad.The olfactory mucosa of mouse is distributed with intensive nerve endings and abundant blood vessel, and its olfactory sensibility exceedes seven to the octuple of dog.The drug law enforcement of training muroid, the explosion-proof advantage with uniqueness.Compared with dog, the sense of smell of drug law enforcement mouse is sensitiveer, and volume is less, convenient, flexible, can arrive and detect the target that dog class cannot arrive at, and be easy to training, its cycle of training is shorter, drops into less, has wide economic and social benefit.Well-trained drug law enforcement mouse is exactly one and small-sizedly investigates inductor flexibly, and it can rely on the nervous system of its prosperity, and the sharp organ such as sense of smell, vision, the sense of hearing rummages up all kinds of drugs and explosive chemical material.
Utilize galvanism or food award to be widely used in the capable training of learning and memory of mouse, or by distinguishing gas with various smell, make mouse and dog etc. form certain conditioned reflex.
If application publication number is the patent of invention of CN101238798A, disclose one " a kind of animal olfaction study training method and system ", comprise the following steps: carry out cutting off the water supply of long period to animal before (1) training, when making training, animal has the hope of strong water detection everywhere; (2) animal is put in training place, during training, the gas of two kinds of different odors is provided in computer control system, and discharges at random in turn; (3), wherein under a kind of smell condition, animal goes to lick water will obtain little water as award; Under another kind of smell condition, animal goes to lick water will obtain electric shock as punishment; (4) like this loop test more than hundred times, if animal capable distinguishes these two kinds different smells, can form correct operant conditioned reflex, can judge whether this animal has the ability distinguishing these two kinds of smells according to the behavior expression of animal.Although this technical scheme may be used for the resolving ability of animal training for different odor, the method training must be carried out in " yearningly feel " of Dependent Animals, and is remembered by " electric shock " or " awards " machine-processed intensification animal, reaches training goal.Apparent, water-cut-off can affect cycle of training, and water-cut-off, by animal individual differentia influence, is difficult to accurate quantification, brings out, cannot bring out the autonomous odor discrimination of animal at all if omit " yearningly feeling ".Therefore the method limited resource is too much, cannot meet actual needs.
If Authorization Notice No. is the utility model of CN202406777U, disclose a kind of " small animal able to programme stimulates stress tests device in terror ", comprise stress tests equipment subsystem and electrical equipment control subsystem two parts, they are arranged in same closed soundproof box; Electrical equipment control subsystem forms electromechanical integration overall structure by electronic stimulation control section and terrified stimulus signal time controling instrument two parts able to programme, and electrical equipment control subsystem is arranged on the top of experimental facilities shielded box.This technical scheme only can make mouse produce stress, cannot set up the conditioned reflex mechanism with subject matter smell, therefore cannot be used for the training of experimental mouse.
Summary of the invention
The object of the invention is to provide a kind of learning and memory training system for mouse identification different odor material.Hungered and thirst by negativity electro photoluminescence or positivity and lure training, make mouse produce behavior reaction to target smell, as identification subject matter mark, the activities such as drug law enforcement and anti-terrorism can be used it for.It is simple that it has structure, stable performance, and cost is low, and training method is simple, and training effectiveness is high, and training cost is low, and its social benefit and economic benefits, have a extensive future.
For achieving the above object, the invention provides following technical scheme: the learning and memory training system of this odoring substance, comprise casing, scented device, air exchange system, electrical stimulation device and control circuit, its technical essential is: described casing is provided with the air inlet and exhaust outlet that are connected with air exchange system, and scented device comprises and the smell passage of casing air inlet relative set, the source of the gas that is connected with smell channel inlet end; Air exchange system comprises gas bomb and exhaust fan; Electrical stimulation device comprises the spacing Turbogrid plates be made up of the positive and negative electrode be arranged alternately in casing; Turbogrid plates are provided with food reward device, are provided with to be positioned at by support spring above Turbogrid plates and cabinet wall hides platform, hide and to be provided with adjustable bottom platform and to hide platform vertically or the support cylinder of level.
For consolidating training effect further, shorten cycle of training, realize the automatic supply of rewarding food, described food reward device comprises top and is provided with the feed box of opening, is positioned at the feeding cylinder of feed box, the extended feed box of output of feeding cylinder simultaneously.
For realize under training period no worker monitor state data record and analysis, whole training system also includes register system, and register system comprises the infrared camera, multifunctional data acquisition card and the video frequency collection card that are positioned at casing.
For realizing the full automatic control under unmanned state, described control circuit comprises time-controlled switch, electrical stimulation circuit, electro photoluminescence control circuit, scented device control circuit, air exchange system control circuit, food reward device control circuit and hides platform control circuit; Electrical stimulation circuit comprise pulse signal source, the positive and negative electrode be arranged alternately be connected with pulse signal source output terminal, for normally opened contact KM31, KM41 of the connection negative or positive electrode of relay coil KM3 and KM4 of control electrode break-make and normally opened contact KM32, KM42 of connection negative pole or positive pole;
The relay coil KM4 that electro photoluminescence control circuit comprises the time-delay relay JS2 ~ JS5 by time-controlled switch control, the relay coil KM3 by time-delay relay coil JS2 normally opened contact JS22 and time-delay relay JS3 normally-closed contact JS31 control, controls by time-delay relay coil JS4 normally opened contact JS41 and time-delay relay JS5 normally-closed contact JS51;
Scented device control circuit comprises the relay coil KM1 controlled by time-delay relay coil JS1 normally-closed contact JS11, magnetic valve JZ1, JZ2 of controlling by relay coil KM1 normally opened contact KM11;
Air exchange system control circuit comprises by time-delay relay coil JS1 and JS2, by the relay coil KM2 of time-delay relay coil JS1 normally opened contact JS12 and time-delay relay coil JS2 normally-closed contact JS21 control, the magnetic valve JZ3 by relay coil KM2 normally opened contact KM21 control;
Food reward device control circuit comprises the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ5 controlled by time-delay relay coil JS5 normally opened contact JS53;
Hide platform control circuit and comprise the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ4 controlled by time-delay relay coil JS5 normally-closed contact JS52.
Beneficial effect of the present invention is: the present invention is intended to design and for training muroid to enforce the drug law and the mouse trainer of explosion-proof purposes, and carries out stimulus signal to experimental mouse and reward mechanism is trained with the coupling of different odor phase fast.Its casing is made up of transparent material, is convenient to observe the activity reaction of mouse in case.Adopt the casing of relative closure, the independence of gas in guard box, prevents case external environment to the interference in casing.Training mouse the smell of subject matter and jump onto certain altitude hide platform or feeding device links together.After the training of fortnight, mouse can have 90% to jump onto feed box to seek food, in this, as observation index, can determine whether there is subject matter (drugs or explosive) in specific region.Its smell passage is many, automatically can control to carry out the training at certain hour interval by time-controlled switch, can be monitored by infrared camera in real time and record training process, more complicated training can be carried out under the condition of a period of time no supervision, only need regular replenishment food.After the training of certain hour, experimental mouse just jumps to immediately to hide on platform and hides electro photoluminescence smelling subject matter smell.Also mouse can be trained to climb up by food, positive sex inducement of drinking water and hide platform.
The conditioned reflex that prior art is formed by training is: 1. yearningly feel → exist target smell → can drink water; 2. yearningly feel → without target smell → can not drink water (drinking-water can be electrically shocked).The conditioned reflex that the present invention is formed by training is: 1. there is target smell → jump onto and hide platform (diving tower is not hidden and can be electrically shocked); 2. without target smell → without any reaction.
The formation of this conditioned reflex visible is more easy, and does not rely on thirsty feel, hungry generation of feeling completely, and limited resource is few, is more conducive to realize, and cycle of training is shorter.Have employed the simpler training system of structure, cost is lower, is more conducive to promote the use of.
As single in stimulus, negative stimulus affects animal exploratory behavior, and learning and memory training disturbing factor is many, delays the realization of goal of animal hope.
Accompanying drawing explanation
Fig. 1 is the structural representation of casing of the present invention;
Fig. 2 is the structural representation of scented device storage tank of the present invention;
Fig. 3 is the control principle schematic diagram of the embodiment of the present invention 1 gas circuit structure;
Fig. 4 is the structural representation of the control circuit of the embodiment of the present invention 1;
Fig. 5 is the control principle schematic diagram of the embodiment of the present invention 2 gas circuit structure;
Fig. 6 is the structural representation of the control circuit of the embodiment of the present invention 2.
Description of reference numerals:
In Fig. 1: 1 feeder; 2 Turbogrid plates; 3 exhaust outlets; 4 casings; 5 smell passages; 6 air inlets; 7 feeding cylinders; 8 feed box; 9 reward food; 10 support cylinder; 11 support springs; 12 hide platform; 13 cover plates; 14 chute-type fixed heads; 15 steady pins;
In Fig. 2: 16 intake interfaces; 17 filler plugs; 18 bases; 19 give vent to anger interface; 20 scent material;
In Fig. 3: 21 exhaust fans; 22 gas cylinders; 23 smell storage tanks; 24 supply fans;
In Fig. 4: KM1 ~ KM4 relay; JS1 ~ JS5 time-delay relay; JZ1 ~ JZ5 magnetic valve; KM11, KM21, KM31, KM32, KM41, KM42, JS12, JS22, JS41, JS53 normally opened contact; JS11, JS21, JS31, JS51, JS52 normally-closed contact;
In Fig. 5: 21 exhaust fans; 22 gas cylinders; 24 supply fans; 25 first smell storage tanks; 26 second smell storage tanks;
In Fig. 6: KM1 ~ KM4 relay; JS1 ~ JS7 time-delay relay; JZ1 ~ JZ6 magnetic valve; KM11, KM21, KM31, KM32, KM41, KM42, JS12, JS22, JS41, JS53, JS61, JS62, JS63 normally opened contact; JS11, JS21, JS31, JS51, JS52, JS71, JS72, JS73, JS74 normally-closed contact.
Embodiment
Below in conjunction with Fig. 1 ~ 6, describe content of the present invention in detail by specific embodiment.
Embodiment 1
The learning and memory training system of this odoring substance, comprises the parts such as the casing 4 be made up of transparent organic glass of relative closure, scented device, electrical stimulation device, food reward device, control circuit and register system.The box like structure with openable lid plate 13 made by casing, experimental mouse and other article (as feed, drinking-water) can be put into or taken out when cover plate 13 is opened.Wherein, electrical stimulation device is the Turbogrid plates 2 be arranged in casing, and Turbogrid plates are made up of the positive and negative copper bar electrode be arranged alternately.Adjacent copper bar forms positive and negative electrode, can form electric current, to stimulate volt training mouse thereon during energising.And the voltage of electrode, size of current are adjustable, to adapt to the training need of different mouse.Food reward device is fixed on the sidewall of casing 4 by hold-down screw, with the distance of Turbogrid plates 2 by chute-type fixed head 14, steady pin 15 is adjustable, it comprises top and is provided with the feed box 8 of opening, is positioned at the feeding cylinder 7 of feed box, the extended feed box of output of feeding cylinder 7.Be provided with adjustable for height being connected to by support spring 11 be arranged on cabinet wall above Turbogrid plates and cabinet wall hides platform 12, hide the height adjustable of platform 12, depend on the physiological conditions (climbing power, body are long) of animal training (experimental mouse).The support cylinder 10 hidden bottom platform can realize the switching of folded state and level, forces the mouse on it to fall rapidly and leaves, so that accelerate frequency of training, improves training observed frequency.Leave certain space between Turbogrid plates and casing, place fecaluria feeder 1 wherein, above it, be covered with bedding and padding.Scented device comprises the smell storage tank 25 be arranged on base 18, and storage tank is provided with feeding port, intake interface 16 and interface 26 of giving vent to anger, and is filled with scent material 25, feeding port is sealed by filler plugs 17 in storage tank.Air exchange system comprises air-source pump (filling the gas cylinder 22 of pressure-air in can selecting), supply fan 26 and exhaust fan 21.Casing is provided with the smell passage (can adopt flexible pipe) be arranged on box cover be connected with the interface of giving vent to anger of scented device, smell channel inlet end is provided with some pores, smell is evenly discharged, casing top is provided with the air inlet 6 be connected with supply fan, and the sidewall of casing 4 bottom is provided with the exhaust outlet 3 be connected with exhaust fan.To get rid of subject matter and odor of excreta in casing, keep the air in mouse training inspection box smooth.Register system can adopt NOLDUS video tracking register system, and this register system mainly comprises the infrared camera being positioned at casing inner top, the video frequency collection card be connected with camera and the multifunctional data acquisition card for combined data.
Control circuit comprises time-controlled switch, electrical stimulation circuit, electro photoluminescence control circuit, scented device control circuit, air exchange system control circuit, food reward device control circuit and hides platform control circuit.
Wherein, electrical stimulation circuit comprise pulse signal source, the positive and negative electrode be arranged alternately be connected with pulse signal source output terminal, for normally opened contact KM31, KM41 of the connection negative or positive electrode of relay coil KM3 and KM4 of control electrode break-make and normally opened contact KM32, KM42 of connection negative pole or positive pole.
The relay coil KM4 that electro photoluminescence control circuit comprises the time-delay relay JS2 ~ JS5 by time-controlled switch control, the relay coil KM3 by time-delay relay coil JS2 normally opened contact JS22 and time-delay relay JS3 normally-closed contact JS31 control, controls by time-delay relay coil JS4 normally opened contact JS41 and time-delay relay JS5 normally-closed contact JS51.
Scented device control circuit comprises the relay coil KM1 controlled by time-delay relay coil JS1 normally-closed contact JS11, magnetic valve JZ1, JZ2 of controlling by relay coil KM1 normally opened contact KM11.
Air exchange system control circuit comprises by time-delay relay coil JS1 and JS2, by the relay coil KM2 of time-delay relay coil JS1 normally opened contact JS12 and time-delay relay coil JS2 normally-closed contact JS21 control, the magnetic valve JZ3 by relay coil KM2 normally opened contact KM21 control.
Food reward device control circuit comprises the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ5 controlled by time-delay relay coil JS5 normally opened contact JS53.
Hide platform control circuit and comprise the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ4 controlled by time-delay relay coil JS5 normally-closed contact JS52.
The circuit control principle of said system is as follows:
The delay time setting time-delay relay coil JS1 ~ JS5 is respectively t1 ~ t5.Wherein, t is the time that time-controlled switch is communicated with; T1 be subject matter smell pass into the time; T2-t1 is the emptying time of breather line; T2 is the time started of first time electro photoluminescence; T3-t2 is the duration of first time electro photoluminescence; T4 is the time started of second time electro photoluminescence; T4-t2 is the interval time of twice electro photoluminescence; T5-t4 is the duration (t5 > t4 > t3 > t2 > t1) of second time electro photoluminescence.
By software systems, time-controlled switch is programmed, when time-controlled switch is on-state, exports the whole circuit of direct current 24V and obtain electric.
1) the passing into of subject matter smell:
During t=0 ~ t1 second, time-delay relay coil JS1 ~ JS5 obtains electric, relay coil KM1 obtains electric, normally opened contact KM11 closes, magnetic valve JZ1, JZ2, JZ4 open, compressed air enters smell tank by magnetic valve JZ1, with the gas of subject matter smell by smell product being sent into casing after magnetic valve JZ2.Support cylinder 10 action, platform will be hidden and be promoted to horizontal level.
During t=t1 second, relay coil KM1 dead electricity, normally-closed contact JS11 disconnects, relay coil KM1 dead electricity, and normally opened contact KM11 disconnects, and magnetic valve JZ1, JZ2 close, and subject matter smell stops sending.
2) removing of subject matter smell:
T=t1(is containing t1 time point) ~ t2 second time, normally opened contact JS12 closes, and relay coil KM2 obtains electric, and normally opened contact KM21 closes, and magnetic valve JZ3 opens, and makes cleaned air enter breather pipe by threeway, by the gas clean-up of residual band smell in systems in which.
3) control of electro photoluminescence:
T=t2(is containing t2 time point) ~ t3 second time, normally-closed contact JS21 disconnects, relay coil KM2 dead electricity, normally opened contact KM21 disconnects, and normally opened contact JS22 closes, and relay coil KM3 obtains electric, normally opened contact KM31, KM32 are closed, and electrode stimulation signal is connected, and first time electro photoluminescence starts.
T=t3(is containing t3 time point) ~ t4 second time, normally-closed contact JS31 disconnects, relay coil KM3 dead electricity, and normally opened contact KM31, KM32 disconnect, and electrode stimulation signal disconnects, and electro photoluminescence for the first time terminates.
T=t4(is containing t4 time point) ~ t5 second time, normally opened contact JS41 closes, and relay coil KM4 obtains electric, and normally opened contact KM41, KM42 are closed, and electrode stimulation signal is connected, and second time electro photoluminescence starts.
During t=t5 second, normally-closed contact JS51 disconnects, relay coil KM4 dead electricity, and normally opened contact KM41, KM42 disconnect, and electrode stimulation signal disconnects, and second time electro photoluminescence terminates.Normally-closed contact JS52 opens simultaneously, and magnetic valve JZ4 closes, and supports cylinder 10 and slowly shrinks, hide platform and return vertical position under support spring effect.Normally opened contact JS53 closes, and magnetic valve JZ5 opens, and the action of feed cylinder, ejects feed box 8 by award food 9.
4) terminate cycle of training:
During t > t5 second, the time-controlled switch of being programmed in advance by software is disconnected, and whole electrical equipment resets, and cycle of training terminates.
In addition, also can comprise food reward step to consolidate the memory of experimental mouse, specifically increase the relay controlling feeding cylinder, namely stop in the electro photoluminescence when t5 starts, the action of feeding cylinder, ejects the food in food boxes, gives food reward.
Below illustrate the training process of the present embodiment:
1) experimental mouse is placed on Turbogrid plates, by connection and the turn-off time (the automatic training under no worker monitor state can be realized) of software set time-controlled switch.When time-controlled switch is communicated with, subject matter smell enters casing, hides platform and be in horizontal position under support cylinder action;
2) after passing into subject matter smell t1 second, scented device is closed, and air exchange system starts, and subject matter smell is emptying within t2-t1 second;
3) air exchange system is closed, after at least postponing t3-t1 second (preferably 3 ~ 6 seconds), electrical stimulation device starts, the first time electric pulse stimulation that the duration is t3-t2 second is carried out when t3 second, bring out " escape mechanism " of experimental mouse, force searching to hide platform, when t4 second, carry out the second time electric pulse stimulation that the duration is t4-t3 second, with consolidating memory;
4) electrical stimulation device is closed, and support cylinder and slowly shrink, hide platform and slowly return to vertical position under support spring effect, food reward device starts, and is ejected by award food, further consolidating memory.
The present embodiment is single subject matter Odor stimulation.In step 3), for the experimental mouse that reaction sensitivity is poor, ensure training effect by the mode increasing electric pulse stimulation number of times or increase pulse current.According to the adjustment of electric current, its technical scheme is comparatively simple, and only needing to increase trimmable resistance can realize, and arranges adjusting knob on the control panel to realize this function.And for increase electric pulse number of times, then need the quantity increasing the electrical equipment such as time-delay relay, contact, add the complexity of circuit.Control according to mcu programming, twice electric pulse is hocketed, be not easy equally to realize, usually do not advise adopting latter two technical scheme.
Training effect judges:
If experimental mouse produces the time of hiding reaction (namely escaping to hiding platform) after smelling subject matter smell be less than about (t3-t1) second, just represent that learning and memory produces a desired effect.Now, 1 ~ 2 week, interval, carry out steadiness training again, several Reaction time shorten repeatedly, until experimental mouse hides reaction immediately after smelling subject matter smell, namely indicate the permanent stabilizing power that experimental mouse has possessed identification subject matter smell and produced the behavior of specific observations index, can come into operation.
Embodiment 2
In addition, also can increase comparison training at arbitrary period, i.e. setting many covers odor source storage tank, alternately in casing, discharge different Odor stimulations, after passing into non-subject matter smell, no current stimulates, without food reward, only hides platform and prop up, hide platform also without food reward even if jump onto, thus consolidate the conditioned reflex with subject matter smell.Shown in Fig. 5,6, the present embodiment increases extra smell storage tank on the basis of embodiment 1, in order to produce the Odor stimulation of non-subject matter.On control circuit, increase time-delay relay JS6, JS7, magnetic valve JZ2 is increased to the normally opened contact JS62 of time-delay relay JS6, the normally-closed contact JS72 of time-delay relay JS7.Magnetic valve JZ3 is increased to the normally-closed contact JS74 of time-delay relay JS7.Magnetic valve JZ4 is increased to the normally opened contact JS63 of time-delay relay JS6, the normally-closed contact JS73 of time-delay relay JS7.Magnetic valve JZ6 is increased to the normally opened contact JS61 of time-delay relay JS6, the normally-closed contact JS71 of time-delay relay JS7.Increasing the quantity of non-subject matter smell storage tank as continued, only need continue the corresponding electrical equipment of parallel increase, can achieve the goal, its circuit structure and gas circuit structure figure are slightly.
The delay time setting time-delay relay coil JS1 ~ JS7 is respectively t1 ~ t7.Wherein, t is the time that time-controlled switch is communicated with; T1 be subject matter smell pass into the time; T2-t1 is the emptying time of breather line; T2 is the time started of first time electro photoluminescence; T3-t2 is the duration of first time electro photoluminescence; T4 is the time started of second time electro photoluminescence; T4-t2 is the interval time of twice electro photoluminescence; T5-t4 is the duration of second time electro photoluminescence; T6 is the time started of non-subject matter Odor stimulation; T7-t6 is the time that passes into (t7 > t6 > t5 > t4 >=t3 > t2 > t1) of non-subject matter smell.
During t=t6 second, normally opened contact JS61, JS62, JS63, JS64 are closed, and magnetic valve JZ2, JZ4, JZ6 open, and scented device starts, and passes in casing by the smell of the second smell storage tank, support cylinder action, will hide platform and prop up to horizontal position.
During t=t7 second, normally-closed contact JS71, JS72, JS73, JS74 disconnect, and magnetic valve JZ2, JZ6 close, and magnetic valve JZ3 opens, and scented device is closed, and air exchange system starts.
Below illustrate the training process of the present embodiment:
1) experimental mouse is placed on Turbogrid plates, by connection and the turn-off time (the automatic training under no worker monitor state can be realized) of software set time-controlled switch.When time-controlled switch is communicated with, the subject matter smell of the first smell storage tank 25 enters casing, hides platform and be in horizontal position under support cylinder action;
2) after passing into subject matter smell t1 second, scented device is closed, and air exchange system starts, and subject matter smell is emptying within t2-t1 second;
3) air exchange system is closed, after at least postponing t3-t1 second (preferably 3 ~ 6 seconds), electrical stimulation device starts, the first time electric pulse stimulation that the duration is t3-t2 second is carried out when t3 second, bring out " escape mechanism " of experimental mouse, force searching to hide platform, when t4 second, carry out the second time electric pulse stimulation that the duration is t4-t3 second, with consolidating memory;
4) electrical stimulation device is closed, and support cylinder and slowly shrink, hide platform and slowly return to vertical position under support spring effect, food reward device starts, and is ejected by award food, further consolidating memory;
5) second smell storage tank 26 smell (non-subject matter smell) of scented device enters casing 4, hides platform and be in horizontal position under support cylinder action;
6), after passing into non-subject matter smell t7-t6 second, scented device is closed, and air exchange system starts, by non-subject matter smell emptying, now only have be in horizontal position hide platform, no current stimulates, without food reward.

Claims (4)

1. the learning and memory training system of an odoring substance, comprise casing, scented device, air exchange system, electrical stimulation device and control circuit, it is characterized in that: described casing is provided with the air inlet and exhaust outlet that are connected with air exchange system, scented device comprises and the smell passage of casing air inlet relative set, the source of the gas that is connected with smell channel inlet end; Air exchange system comprises gas bomb and exhaust fan; Electrical stimulation device comprises the spacing Turbogrid plates be made up of the positive and negative electrode be arranged alternately in casing; Turbogrid plates are provided with food reward device, are provided with to be positioned at by support spring above Turbogrid plates and cabinet wall hides platform, hide and to be provided with adjustable bottom platform and to hide platform vertically or the support cylinder of level.
2. the learning and memory training system of the odoring substance according to claims 1, is characterized in that: described food reward device comprises top and is provided with the feed box of opening, is positioned at the feeding cylinder of feed box, the extended feed box of output of feeding cylinder.
3. the learning and memory training system of the odoring substance according to claims 1, is characterized in that: also comprise register system, and register system comprises the infrared camera, multifunctional data acquisition card and the video frequency collection card that are positioned at casing.
4. the learning and memory training system of the odoring substance according to claims 1 or 2 or 3, is characterized in that: described control circuit comprises time-controlled switch, electrical stimulation circuit, electro photoluminescence control circuit, scented device control circuit, air exchange system control circuit, food reward device control circuit and hides platform control circuit;
Electrical stimulation circuit comprise pulse signal source, the positive and negative electrode be arranged alternately be connected with pulse signal source output terminal, for normally opened contact KM31, KM41 of the connection negative or positive electrode of relay coil KM3 and KM4 of control electrode break-make and normally opened contact KM32, KM42 of connection negative pole or positive pole;
The relay coil KM4 that electro photoluminescence control circuit comprises the time-delay relay JS2 ~ JS5 by time-controlled switch control, the relay coil KM3 by time-delay relay coil JS2 normally opened contact JS22 and time-delay relay JS3 normally-closed contact JS31 control, controls by time-delay relay coil JS4 normally opened contact JS41 and time-delay relay JS5 normally-closed contact JS51;
Scented device control circuit comprises the relay coil KM1 controlled by time-delay relay coil JS1 normally-closed contact JS11, magnetic valve JZ1, JZ2 of controlling by relay coil KM1 normally opened contact KM11;
Air exchange system control circuit comprises by time-delay relay coil JS1 and JS2, by the relay coil KM2 of time-delay relay coil JS1 normally opened contact JS12 and time-delay relay coil JS2 normally-closed contact JS21 control, the magnetic valve JZ3 by relay coil KM2 normally opened contact KM21 control;
Food reward device control circuit comprises the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ5 controlled by time-delay relay coil JS5 normally opened contact JS53;
Hide platform control circuit and comprise the time-delay relay coil JS5 controlled by time-controlled switch, the magnetic valve JZ4 controlled by time-delay relay coil JS5 normally-closed contact JS52.
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CN105900854A (en) * 2016-07-11 2016-08-31 重庆市合川区公安局 Gog odor search training system and odor collecting distributor
CN106614068A (en) * 2017-01-05 2017-05-10 沈阳医学院 Active running wheel odor identification exercise box
CN107410080A (en) * 2017-08-14 2017-12-01 沈阳医学院 Quick-fried police dog standardized training device is searched in drug law enforcement
CN107432254A (en) * 2017-09-18 2017-12-05 中国人民武装警察部队黑龙江省边防总队哈尔滨警犬训练基地 Police dog professional training system
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CN115226648B (en) * 2022-07-18 2023-09-22 陕西师范大学 Small-sized automatic animal behavior training system and training method

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