CN105543310A - Method for producing starch sugar - Google Patents
Method for producing starch sugar Download PDFInfo
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- CN105543310A CN105543310A CN201610086265.7A CN201610086265A CN105543310A CN 105543310 A CN105543310 A CN 105543310A CN 201610086265 A CN201610086265 A CN 201610086265A CN 105543310 A CN105543310 A CN 105543310A
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- Prior art keywords
- liquid
- amylose
- solution
- saccharification
- maize straw
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Life Sciences & Earth Sciences (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for producing starch sugar, which comprises the following steps: 1) raw material treatment: carrying out hot grinding treatment on corn straws, mixing the corn straw slurry subjected to hot grinding with corn starch slurry to obtain a slurry mixture; 2) liquification: adding a liquifying enzyme into the slurry mixture to perform liquification, thereby obtaining the liquification solution, wherein the liquifying enzyme comprises cellulase and amylase; 3) saccharification: adding a saccharifying enzyme into the liquification solution to perform saccharification, thereby obtaining the saccharification solution; 4) purification: carrying out membrane separation on the saccharification solution to obtain a clear sugar solution; 5) decolorization and ion exchange: decolorizing the clear sugar solution through powdery activated carbon, and refining through an ion exchange resin to obtain a refined sugar solution; and 6) curing the refined sugar solution to obtain the starch sugar. The method can utilize a certain amount of corn straws as the raw material in the starch sugar production process, and is capable of lowering the raw material cost and increasing the benefits.
Description
Technical field
The present invention relates to a kind of method of producing β-amylose.
Background technology
Glucose industry is the bionic important component part of high-tech, is technology and the basic substance of fermentation industry goods and even numerous food product, is the maximum product of starch deep processing output.Its consumer field is wide, and consumption quantity is large, for promoting the development of foodstuffs industry and promoting to drive Agricultural Industrialization Development to make significant contribution with biotechnology.When China's sucrose still can not be met the need of market, the market of developing into of β-amylose provides polysaccharide source, and this is for price of stabilizing the market, and drives increasing peasant income, promotes agricultural and foodstuffs industry coordinated development important in inhibiting.β-amylose as novel sweetener more and more widespread use in the food industry, not only improve the quality of foodstuffs industry, and functional starch sugar also improves people ' s health quality.
Producing β-amylose in prior art is all adopt the W-Gum or tapioca (flour) that starch content is high, and raw materials cost is high.And on the other hand, the Mierocrystalline cellulose containing higher proportion in maize straw discarded in agriculture production and starch, but utilization ratio is always lower, has both caused environmental problem, wastes ample resources again.
Summary of the invention
Object of the present invention is just to overcome above-mentioned deficiency, provides a kind of method of producing β-amylose, specifically comprises the following steps:
1) Feedstock treating, carries out defibrator process process by maize straw, and the maize straw slip after defibrator process mixes obtained mixed slurry with corn starch liquid;
2) liquefy, in mixed slurry, add α-amylase liquefy, obtain liquefier, described α-amylase comprises cellulase and amylase;
3) saccharification, adds saccharifying enzyme in liquefier, carries out saccharification, obtains saccharified liquid;
4) purify, described saccharified liquid is carried out membrane sepn, obtains clarifying sugar liquid;
5) decolouring and ion-exchange, described clarifying sugar liquid through powder activity carbon decoloring, then is refined through ion exchange resin, obtains refining liquid glucose;
6) refined sugar liquid-solidization is obtained β-amylose.
Further, the maize straw in described step 1) first removes epidermis before carrying out defibrator process process.
Further, the maize straw slip in described step 1) after defibrator process is 1:2 ~ 1:4 with the mixing quality ratio of corn starch liquid, and the concentration of described corn starch liquid is 25 ~ 35wt%.
Further, described step 2) in liquefaction be steam ejection liquefaction.
Further, the membrane sepn in described step 4) is two-stage membrane sepn.
Useful point of the present invention is: utilizing a certain amount of maize straw as raw material when producing β-amylose, can reduce raw materials cost, improve effects.Owing to containing a large amount of Mierocrystalline cellulose and xylogen in maize straw, more difficult decomposition, when being used alone maize straw, because bed material concentration is too low, can not excite diastatic activity, conversion coefficient is extremely low, can not produce economic benefit.Use a certain proportion of maize straw and W-Gum mixture manufacturing β-amylose, starch content in mixed slurry still can remain on proper level, the activity of α-amylase and saccharifying enzyme can be ensured, enable the starch in maize straw and Mierocrystalline cellulose thoroughly enzymolysis and conversion, maize straw is in advance through defibrator process process simultaneously, promote Mierocrystalline cellulose and lignin separation, be also beneficial to follow-up enzymolysis.
Can solve agriculture production maize straw to a certain extent discards the environmental problem caused simultaneously in the present invention, improves the utility value of maize straw.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
A production method for β-amylose, comprises the following steps:
1) Feedstock treating, carries out defibrator process process by maize straw, and the maize straw slip after defibrator process and concentration are that the ratio of the corn starch liquid 1:2 in mass ratio of 25wt% mixes obtained mixed slurry;
2) liquefy, in mixed slurry, add α-amylase liquefy, obtain liquefier, described α-amylase comprises cellulase and amylase;
3) saccharification, adds saccharifying enzyme in liquefier, carries out saccharification, obtains saccharified liquid;
4) purify, described saccharified liquid is carried out membrane sepn, obtains clarifying sugar liquid;
5) decolouring and ion-exchange, described clarifying sugar liquid through powder activity carbon decoloring, then is refined through ion exchange resin, obtains refining liquid glucose;
6) refined sugar liquid-solidization is obtained β-amylose.
Embodiment 2
A production method for β-amylose, comprises the following steps:
1) Feedstock treating, removes epidermis by maize straw, obtains maize straw core, then carries out defibrator process process, and the maize straw slip after defibrator process and concentration are that the ratio of the corn starch liquid 1:4 in mass ratio of 35wt% mixes obtained mixed slurry;
2) liquefy, in mixed slurry, add α-amylase carry out steam ejection liquefaction, obtain liquefier, described α-amylase comprises cellulase and amylase;
3) saccharification, adds saccharifying enzyme in liquefier, carries out saccharification, obtains saccharified liquid;
4) purify, described saccharified liquid is carried out microfiltration membrane separation and Ultra filtration membrane, obtains clarifying sugar liquid;
5) decolouring and ion-exchange, described clarifying sugar liquid through powder activity carbon decoloring, then is refined through ion exchange resin, obtains refining liquid glucose;
6) refined sugar liquid-solidization is obtained β-amylose.
Obtained β-amylose DE value is 98%.
Claims (5)
1. produce a method for β-amylose, it is characterized in that, comprise the following steps:
1) Feedstock treating, carries out defibrator process process by maize straw, and the maize straw slip after defibrator process mixes obtained mixed slurry with corn starch liquid;
2) liquefy, in mixed slurry, add α-amylase liquefy, obtain liquefier, described α-amylase comprises cellulase and amylase;
3) saccharification, adds saccharifying enzyme and carries out saccharification, obtain saccharified liquid in liquefier;
4) purify, described saccharified liquid is carried out membrane sepn, obtains clarifying sugar liquid;
5) decolouring and ion-exchange, described clarifying sugar liquid through powder activity carbon decoloring, then is refined through ion exchange resin, obtains refining liquid glucose;
6) refined sugar liquid-solidization is obtained β-amylose.
2. a kind of method of producing β-amylose according to claim 1, is characterized in that: the maize straw in described step 1) first removes epidermis before carrying out defibrator process process.
3. a kind of method of producing β-amylose according to claim 1, is characterized in that: the maize straw slip in described step 1) after defibrator process is 1:2 ~ 1:4 with the mixing quality ratio of corn starch liquid, and the concentration of described corn starch liquid is 25 ~ 35wt%.
4. a kind of method of producing β-amylose according to claim 1, is characterized in that: described step 2) in liquefaction be steam ejection liquefaction.
5. a kind of method of producing β-amylose according to claim 1, is characterized in that: the membrane sepn in described step 4) is two-stage membrane sepn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610086265.7A CN105543310A (en) | 2016-02-16 | 2016-02-16 | Method for producing starch sugar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610086265.7A CN105543310A (en) | 2016-02-16 | 2016-02-16 | Method for producing starch sugar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105543310A true CN105543310A (en) | 2016-05-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610086265.7A Pending CN105543310A (en) | 2016-02-16 | 2016-02-16 | Method for producing starch sugar |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441531A (en) * | 2018-03-13 | 2018-08-24 | 长春市吉达自动化系统有限公司 | A kind of method that starch sugar production automation is realized |
| CN115011651A (en) * | 2022-07-11 | 2022-09-06 | 雄安创新研究院 | Method for efficiently preparing sugar by using reed |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101090962A (en) * | 2004-11-29 | 2007-12-19 | 艾尔萨姆工程有限公司 | Enzymatic hydrolysis of biomasses having a high dry matter (DM) content |
| CN102337316A (en) * | 2011-09-30 | 2012-02-01 | 广州双桥股份有限公司 | Process for producing starch sugar |
| CN103525887A (en) * | 2013-10-21 | 2014-01-22 | 诸城东晓生物科技有限公司 | Energy-saving environment-friendly crystalline dextrose production method |
| CN105316374A (en) * | 2014-07-11 | 2016-02-10 | 武汉中粮食品科技有限公司 | Sugar making method |
-
2016
- 2016-02-16 CN CN201610086265.7A patent/CN105543310A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101090962A (en) * | 2004-11-29 | 2007-12-19 | 艾尔萨姆工程有限公司 | Enzymatic hydrolysis of biomasses having a high dry matter (DM) content |
| CN102337316A (en) * | 2011-09-30 | 2012-02-01 | 广州双桥股份有限公司 | Process for producing starch sugar |
| CN103525887A (en) * | 2013-10-21 | 2014-01-22 | 诸城东晓生物科技有限公司 | Energy-saving environment-friendly crystalline dextrose production method |
| CN105316374A (en) * | 2014-07-11 | 2016-02-10 | 武汉中粮食品科技有限公司 | Sugar making method |
Non-Patent Citations (2)
| Title |
|---|
| MOSHE KATZ等: "Production of Glucose by Enzymatic Hydrolysis", 《APPLIED MICROBIOLOGY》 * |
| 齐义鹏: "《纤维素酶及其应用》", 30 April 1980, 四川人民出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441531A (en) * | 2018-03-13 | 2018-08-24 | 长春市吉达自动化系统有限公司 | A kind of method that starch sugar production automation is realized |
| CN115011651A (en) * | 2022-07-11 | 2022-09-06 | 雄安创新研究院 | Method for efficiently preparing sugar by using reed |
| CN115011651B (en) * | 2022-07-11 | 2024-04-12 | 雄安创新研究院 | Method for efficiently preparing sugar by reed |
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Application publication date: 20160504 |