CN105543090B - A kind of dehydration device and method - Google Patents
A kind of dehydration device and method Download PDFInfo
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- CN105543090B CN105543090B CN201610033982.3A CN201610033982A CN105543090B CN 105543090 B CN105543090 B CN 105543090B CN 201610033982 A CN201610033982 A CN 201610033982A CN 105543090 B CN105543090 B CN 105543090B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention provides a kind of dehydration device and methods, belong to biotechnology.The dehydration device includes shell, main shaft push rod, magnetosheath and electric device；Main shaft push rod runs through shell, and both ends protrude from shell；And main shaft push rod can be moved back and forth along shell；One end of main shaft push rod is provided with magnetic pole；The magnetosheath enclosure is in magnetic pole, and magnetic pole is coaxially disposed with magnetosheath, and the center in the magnetosheath, and one end of magnetosheath opening is connected on shell；Electric device setting is connect in shell, and with magnetosheath, for making magnetosheath rotate.The centrifugal force interaction generated when the device is by rotating the centripetal force of magnetic pole and magnetosheath, to realize the disengaging of liquid contained by magnetic bead and its surface, it is easier than dewatering types such as general drying, winds, quick, and the substance detached will not be damaged.
Description
Technical field
The present invention relates to biotechnologies, in particular to a kind of dehydration device and method.
Background technology
In biotechnology, dehydration is critically important in biological substance separation process but very scabrous difficult together
Topic.It is due to being carried out under the conditions of biological substance separation process is existing for liquid mostly why dehydration, which is referred to as " problem ",
's；Lymphocyte, cancer cell are detached such as from blood；Certain bacterium, virus or other harmful substances etc. are detached from milk
Deng.The substance separated is always more or less with the remaining unnecessary liquid from upstream liquid；And in order to go
Except these surplus liquid substances, conventional laboratory methods mostly use high temperature drying, room temperature volatilization, delay waiting, air flowing (wind
Blow) etc. post-processing means, it is clear that the defects of time-consuming, bothersome and cumbersome can be caused.
For example, during extracting nucleic acid using paramagnetic particle method, the nucleic acid of release is inhaled cell cracking, magnetic bead by lysate
It is attached, it needs to detach magnetic bead from lysate；And way conventional in currently available technology is typically all additional by one
Magnetic field make magnetic bead be gathered in the side of container (be equipped with lysate and magnetic bead, generally centrifuge tube)；Then it recycles and moves
Liquid rifle absorbs lysate, to realize the separation of magnetic bead and lysate；The magnetic bead detached further passes through elution, to
Realize the extraction of purpose nucleic acid.
However, in the extracting method, by lysate and during being adsorbed with the Beads enrichment of nucleic acid, in the magnetic bead of aggregation
A large amount of liquid can be remained；Therefore, separation of solid and liquid is not thorough, to reduce the purity of the nucleic acid subsequently extracted.If to magnetic
Pearl flows the further dehydrating operations such as (wind), Adsorption of Filter Paper using air, on the one hand time-consuming, laborious, inconvenient；It is another
Aspect, it is also possible to adsorbed nucleic acid can be caused to be destroyed or be lost in.
In view of this, special propose the present invention.
Invention content
The first object of the present invention is to provide a kind of dehydration device, to solve to contain in existing biological substance isolation technics
There is its separation of solid and liquid of the magnetic bead of separated object matter to be not thorough (technical barrier containing the liquid being difficult to clean off in separated object matter),
And the technical problem that dehydrating operations are time-consuming, laborious, the device is by generating the centripetal force of magnetic pole and while being rotated with magnetosheath
Centrifugal force be combined, to realize the disengaging of magnetic bead and magnetic bead surfaces contained humidity, than dehydrations sides such as general drying, winds
Formula is easy, quick, and will not be damaged to the substance detached.
The second object of the present invention is to provide a kind of method being dehydrated using the dehydration device, this method operation letter
Just, dehydration efficiency is high, takes short.
In order to realize that the above-mentioned purpose of the present invention, spy use following technical scheme：
The present invention provides a kind of dehydration device, the dehydration device includes shell, main shaft push rod, magnetosheath and electronic dress
It sets；
The main shaft push rod runs through the shell, and both ends protrude from the shell；And the main shaft push rod being capable of edge
Shell reciprocating motion；One end of the main shaft push rod is provided with magnetic pole；The magnetosheath enclosure in the magnetic pole, and magnetic pole with
The magnetosheath coaxial arrangement, and the center in the magnetosheath, one end connection of the magnetosheath opening is on the housing；It is described
Electric device setting is connect in the shell, and with the magnetosheath, for making the magnetosheath rotate.
For above-mentioned device, the magnetic centripetal force and magnetosheath that are characterized by magnetic pole generation rotate the centrifugal force generated
Interaction, so the position relationship of magnetic pole and magnetosheath is critically important.Magnetic pole is arranged in the center of the magnetosheath device
(the two is coaxially disposed, and whether no matter whether magnetic pole stretches into the blind end of magnetosheath, and magnetic pole is constantly in the center of magnetosheath),
And then a magnetic centripetal force is provided to the magnetic bead of magnetosheath blind end, the centrifugal force generated when centripetal force and magnetosheath rotation reaches
When a certain equalization point, magnetic bead is rested on magnetosheath blind end by the effect of centripetal force, liquid got rid of by centrifugation force effect it is de-, to reach
To the effect of dehydration.
Specifically, this dehydration device provided by the invention, agent structure is a shell, on shell there are one by its
The main shaft push rod that runs through and can be moved back and forth along shell；Under the effect of external force, the magnetic pole being arranged on main shaft push rod can
Bottom to extend into magnetosheath (by the returning device being additionally arranged, may make magnetic pole further to return, to realize that main shaft pushes away
The back and forth movement of bar and magnetic pole)；And the electric device in shell, which is arranged, then can make magnetosheath rotate movement.
During being applied the dehydration device, separation magnetic bead lysate is such as used it for (containing having adsorbed core
The magnetic bead and lysate of acid)；Magnetosheath is extend into mixed system, pushes main shaft push rod at this time so that stretch the end of magnetic pole
Enter to the bottom of magnetosheath；Under the action of magnetic pole, the magnetic bead in mixed system has been adsorbed on magnetosheath, is then removed, from
And realize the quick separating of magnetic bead and mixed system.Further, it is that can rotate under the drive of electric device due to magnetosheath
, the operation that the magnetic bead adsorbed in this way realizes similar centrifugal dehydration during rotation (can be by controlling magnetosheath
Rotating speed realizes the adjusting of centrifugal force) so that the liquid phase contained by inside magnetic bead is further detached.After the completion of centrifugation, go
Fall to be applied to the power of main shaft push rod, main shaft push rod return under the action of returning device drives magnetic pole to be left from the bottom of magnetosheath；
The magnetic bead adsorbed can carry out the operation of next step due to losing the suction-operated of magnetic force to be detached from from upper magnetosheath.
As it can be seen that this dehydration device provided by the invention, makes dehydration greatly simplify, substantially reduces dehydration
Time, in the case of skilled operation, dehydration can be completed in a few seconds, and the process being entirely dehydrated will not be to being detached
Substance causes to damage.In addition, the dehydration device is compact-sized, it is highly convenient for aseptically operating.
Optionally, further include magnetosheath connection ring；Magnetosheath connection ring setting on the housing, and one end with it is described
Magnetosheath is socketed；The other end is connect with the electric device.
In the present invention, magnetosheath connection ring plays the role of magnetosheath with electric device being connected, thus more easily
Realize that electric device drives the effect of magnetosheath rotation.
Optionally, further include returning device；The returning device is arranged in the shell；And for making the main shaft push away
Bar is moved to the direction far from the magnetosheath.
The effect of returning device is so that main shaft push rod return, i.e., so that magnetic pole is left from the bottom of magnetosheath, and then improve
The effect of automation mechanized operation.For the concrete structure of returning device, it is preferably provided to an one end and is fixed with main shaft push rod, it is another
End and the fixed spring of shell.Pass through returning device and the pressure additionally applied to main shaft push rod, you can realize main shaft push rod
(subsidiary magnetic pole) back and forth movement.
Optionally, it is additionally provided with circuit board in the shell；The electric device and the circuit board electrical connection, the electricity
The motor adjusting knob and charge port for protruding from the shell are additionally provided on the plate of road；It is additionally provided with and is used on the shell
The button switch of control circuit board power supply.
Circuit board can integrate various circuits, by motor adjusting knob, it can be achieved that the adjusting of the output power of electric device,
And magnetosheath connection ring and the slewing rate of magnetosheath are directly controlled；Realize the rotational speed regulation of magnetosheath；And by charge port (for side
Just for the sake of, can be set to USB interface), power supply (interior to be set to shell) can be given to realize charging effect；In addition, being also set up on shell
It is useful for the button switch of control circuit board power supply.
Optionally, the one end open of the magnetosheath, the other end closing, and the magnetosheath cross-sectional area along from open end to
The direction of blind end is tapered.
During dehydration, magnetosheath can be extend into mixture system, and outer wall can hang with water droplet.Therefore, in order to carry
The cross-sectional area of magnetosheath is preferably arranged to along the tapered form in the direction from open end to blind end by the effect of height dehydration,
During rotating centrifugation, the magnetosheath with the structure is detached from from top to bottom convenient for adhered to water, to improve dehydration effect
Rate.
Optionally, multiple protrusions are provided on the outer wall of the closed one end of the magnetosheath.
Optionally, the number for stating protrusion is even number, and is symmetrical arranged along the direction of the magnetosheath axis.
It can revolve, it is equal the distance between per two adjacent protrusions.
In addition, in order to increase grinding effect of the magnetosheath in rotation process, it is preferable that the outer surface of magnetosheath to be arranged to slightly
Face.It is further preferred that in order to improve mixing effect of the magnetosheath in rotation process, can the cecum of magnetosheath be provided with a word
Type or cross small-sized fan blade.
The method of the dehydration device dehydration, includes the following steps：
1) main shaft push rod, is pushed, so that the magnetic pole is reached the blind end of the magnetosheath, then stretches the magnetosheath of dehydration device
Enter in the solid-liquid mixture containing magnetisable material；
2), magnetisable material is adsorbed to the blind end of magnetosheath under the action of the magnetic pole, then by the dehydration device
It is removed from solid-liquid mixture；
3) electric device, is controlled so that magnetosheath rotates, and magnetisable material is kept absorbed state, magnetic by magnetic fields
Bead surface contained humidity is got rid of de- under the influence of centrifugal force.
The dewatering is completed using special dehydration device, for the solid-liquid mixture containing magnetisable material,
The separation of magnetic bead and magnetic bead surfaces liquid can be realized between the several seconds, and the substance detached will not be caused to damage,
It is saved greatly the time, and improves efficiency.
Optionally, in step 1), the solid-liquid mixture includes DNA lysates or eluent containing magnetic bead.
For including DNA lysates or eluent containing magnetic bead, it is highly suitable as the processing of the dehydration device
Object, magnetic bead are adsorbed and are removed in can all being embodied in a short period of time from mixing, and the process of rotating centrifugal also generally may be used
It is completed between the several seconds, after discharging magnetic force, magnetic bead can carry out next step operation process, again by Beads enrichment after elution, you can
Purpose nucleic acid is obtained, and magnetic bead is separately stored after centrifugal dehydration or is utilized again.
Using above-mentioned device, the present invention can solve insurmountable problem in the existing field.When utilizing nanometer magnetic bead
When detaching biological substance, it is to reach the blind end offer magnetic field of the magnetosheath to push the main shaft push rod of the present apparatus, the magnetic pole,
Magnetosheath is extend into the mixing liquid containing magnetic bead as will be described at this time, and magnetic bead can be attracted to outside the blind end of the magnetosheath
Side；Since the magnetic pole is located at the center of the magnetosheath, for magnetic bead, the magnetic pole provide a magnetism to
Mental and physical efforts.The blind end of magnetosheath proposes liquid level as will be described, and starts rotation button, and magnetosheath is made to generate rotary motion, rotation at this time
Transhipment is dynamic will to generate centrifugal force to the liquid of the magnetic bead and its surface.Since magnetic bead keeps former under the centripetal force effect in magnetic field
Position, liquid are not influenced by magnetic force then and are got rid of by centrifugal force de-, and the interaction of this centrifugal force and centripetal force makes magnetic bead and magnetic
Bead surface liquid plays separating effect.Entire dehydration can be completed in a few seconds.
Compared with prior art, beneficial effects of the present invention are：
(1), the device is by the way that the magnetic force of magnetic pole and the centrifugal force generated when magnetosheath rotates to be combined, to real
The disengaging of existing magnetic bead contained humidity, it is easier than dewatering types such as general drying, winds, quick, and will not be to being detached
Substance damages.
(2), since the dehydration device is compact-sized portable, it is highly suitable as the experimental tool of field test, together
When be also highly convenient for aseptically being operated.
(3), the outer surface of magnetosheath is further improved and (is such as arranged to asperities, increases fan blade) and may make that this is de-
Water installations realize grinding, the effect of agitating device can be used as a kind of well-adapted reality in field of biology with multifunctionality
Test tool.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described.
Fig. 1 is the structural schematic diagram of the dehydration device in preferred embodiment provided by the invention；
Fig. 2 is the structural schematic diagram of the dehydration device in another technical solution of the present invention；
Reference numeral：
Shell -101；Main shaft push rod -102；Magnetosheath -103；Electric device -104；Magnetic pole -105；Magnetosheath connection ring -201；
Returning device -202；Button switch -203；Protrusion -204.
Specific implementation mode
Please refer to Fig.1-Fig. 2, this dehydration device provided by the invention comprising shell 101, main shaft push rod 102, magnetosheath
103 and electric device 104；
Main shaft push rod 102 runs through shell 101, and both ends protrude from shell 101；And main shaft push rod 102 can be along shell
Body 101 moves back and forth；One end of main shaft push rod 102 is provided with magnetic pole 105；103 enclosure of magnetosheath is in (specific such as Fig. 2 in magnetic pole 105
It is shown), and one end of its opening is connected on shell 101；Electric device 104 be arranged in shell 101, and with magnetosheath 103
Connection, for making magnetosheath 103 rotate.
This dehydration device provided by the invention, agent structure are a shell 101, on shell 101 there are one by its
The main shaft push rod 102 that runs through and can be moved back and forth along shell 101；Under the effect of external force, it is arranged on main shaft push rod 102
Magnetic pole 105 can extend into the bottom of magnetosheath 103；And the electric device 104 in shell 101, which is arranged, can then make magnetosheath
103 rotate.
During being applied the bio-separation device, separation magnetic bead lysate is such as used it for (containing absorption
The magnetic bead and lysate of nucleic acid)；Magnetosheath 103 is extend into mixed system, pushes main shaft push rod 102 at this time so that magnetic
The end of column 105 has extend into the bottom of magnetosheath 103；Under the action of magnetic pole 105, the magnetic bead in mixed system is adsorbed to
It on magnetosheath 103, then removes, to realize the quick separating of magnetic bead and mixed system.Further, since magnetosheath 103 is in electricity
It is rotary under the drive of dynamic device 104, is attached to the magnetic bead on the outside of 103 blind end of magnetosheath and is retained under the magnetic force
On magnetosheath 103, and the liquid of magnetic bead surfaces then under the influence of centrifugal force with Beads enrichment.
After the completion of centrifugation, remove the power for being applied to main shaft push rod 102, main shaft push rod 102 is under the action of returning device 202
Return drives magnetic pole 105 to be left from the bottom of magnetosheath 103；The magnetic bead adsorbed due to lose the suction-operated of magnetic force to
It is detached from from upper magnetosheath 103, and the operation of next step can be carried out very conveniently.
As it can be seen that this dehydration device provided by the invention, makes dehydration greatly simplify, substantially reduces dehydration
Time, the process that dehydration can be completed, and is entirely dehydrated in a few seconds will not cause to damage to the substance detached.Separately
Outside, the dehydration device is compact-sized, is highly convenient for aseptically operating.
On the basis of above-mentioned technical proposal, it is further preferred that further including magnetosheath connection ring 201；Magnetosheath connection ring
201 are arranged on shell 101, and one end is socketed with magnetosheath 103；The other end connect (namely magnetosheath 103 with electric device 104
It is realized and is connected by magnetosheath connection ring 201 and electric device 104, electric device 104 is real by directly driving magnetosheath connection ring 201
Magnetosheath 103 is now set to rotate movement).
Magnetosheath connection ring 201 plays the role of magnetosheath 103 with electric device 104 being connected, thus more easily real
Existing electric device 104 drives the effect that magnetosheath 103 rotates.
In biological experiment, for various operation equipment, the height of the degree of automation directly affects entire experiment
Success or failure and efficiency；Therefore, further excellent on the basis of said program in order to improve the automation performance of the device
Choosing, further include returning device 202；Returning device 202 is arranged in shell 101；And for making main shaft push rod 102 to far from magnetic
The direction movement of set 103；It is additionally provided with circuit board in shell 101；Electric device 104 and circuit board electrical connection, on circuit board also
It is provided with the motor adjusting knob and charge port for protruding from shell 101；It is additionally provided with for control circuit board on shell 101
The button switch 203 of power supply.
The effect of returning device 202 be so that 102 return of main shaft push rod, i.e., so that magnetic pole 105 from the bottom of magnetosheath 103 from
It opens, and then improves the effect of automation mechanized operation.Pass through returning device 202 and the additional power for being applied to main shaft push rod 102, you can
Realize that main shaft push rod 102 and magnetic pole 105 are moved back and forth along shell 101.
For the concrete structure of returning device 202, it is preferably provided to an one end and is fixed with main shaft push rod 102, the other end
With 101 fixed spring of shell.
In addition, it can also be a spring being set on main shaft push rod 102, one end passes through connector and main shaft push rod
102 fix, and the other end is fixed with shell 101.
Circuit board, motor adjusting knob and button switch 203 can control the working condition of entire dehydration device, into one
Facilitate to step user's use.
In another preferred technical solution provided by the invention, dehydration device includes：Shell 101, main shaft push rod 102,
Magnetosheath 103 and electric device 104, magnetosheath connection ring 201, returning device 202；
Main shaft push rod 102 runs through shell 101, and both ends protrude from shell 101；And main shaft push rod 102 can be along shell
Body 101 moves back and forth；One end of main shaft push rod 102 is provided with magnetic pole 105；103 enclosure of magnetosheath is in magnetic pole 105, and it is open
One end be connected on shell 101；The setting of electric device 104 is connect in shell 101, and with magnetosheath 103, for making magnetosheath
103 rotate；
Magnetosheath connection ring 201 is arranged on shell 101, and one end is socketed with magnetosheath 103；The other end and electric device
104 connections；Returning device 202 is arranged in shell 101；And for making main shaft push rod 102 be transported to the direction far from magnetosheath 103
It is dynamic；
It is additionally provided with circuit board in shell 101；Electric device 104 and circuit board electrical connection are additionally provided on circuit board
Protrude from the motor adjusting knob and charge port of shell 101；Be additionally provided on shell 101 for control circuit board power supply by
Button switch 203；
The one end open of magnetosheath 103, other end closing, and the cross-sectional area of magnetosheath 103 is along from open end to blind end
Direction is tapered；It is provided with multiple raised 204 on the outer wall of 103 closed one end of magnetosheath；The number of protrusion 204 is even number, and
It is symmetrical arranged along the direction of 103 axis of magnetosheath；It is equal the distance between per two adjacent protrusions 204.
Dehydration device with above structure, it is multi-functional to integrate dehydration, stirring, vortex etc., meets a variety of
The needs of Bioexperiment.In addition if necessary so that the device has grinding function, then accordingly by the appearance of the magnetosheath 103
Face is arranged to asperities, if to realize better mixing effect, "-" type or cross are added in the cecum of magnetosheath 103
The small-sized fan blade of type.
The present invention also provides a kind of methods being dehydrated using the dehydration device, specifically include following steps：
S1, main shaft push rod 102 is pushed, the magnetic pole 105 is made to reach the blind end of the magnetosheath 103, then fill dehydration
The magnetosheath 103 set stretches into the solid-liquid mixture containing magnetisable material；
In this step, solid-liquid mixture includes DNA lysates or eluent containing magnetic bead.
S2, magnetisable material are adsorbed to the blind end of magnetosheath 103 under the action of the magnetic pole 105, then will be described de-
Water installations are removed from solid-liquid mixture；
In this step, the effect for directly removing the magnetic bead for having adsorbed separated object matter from mixture system is realized
Fruit；Which has apparent superiority, after detaching first compared with traditional mode for removing liquid phase from mixture system
Liquid phase remaining in magnetic bead is less (can be vacated naturally under gravity in operating process)；In addition, overcoming liquid in the prior art
Mutually absorb and existing magnetic bead can the loss of energy defect.
S3, control electric device 104 so that magnetosheath 103 rotates, and magnetisable material is kept by magnetic fields by adsorption-like
State, magnetic bead surfaces contained humidity are got rid of de- under the influence of centrifugal force.
This method is directly removed the magnetic bead detached, to carry based on special dehydration device from mixed liquor embodiment
The high effect of dehydration, in addition, during magnetosheath 103 rotates, remaining moisture can be thrown out of, and be further increased de-
The effect of water, and the dewatering can be completed using a device, and device and method are combined so that the operation of dehydration exists
It can efficiently be completed in several seconds, dehydration will not cause separated object matter any damage；Therefore there is extraordinary application
Foreground.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of dehydration device, which is characterized in that the dehydration device includes：Shell, main shaft push rod, magnetosheath and electric device；
The main shaft push rod runs through the shell, and both ends protrude from the shell；And the main shaft push rod can be along shell
Body moves back and forth；One end of the main shaft push rod is provided with magnetic pole；The magnetosheath enclosure in the magnetic pole, and magnetic pole with it is described
Magnetosheath is coaxially disposed, and the center in the magnetosheath, and one end connection of the magnetosheath opening is on the housing；
The electric device setting is connect in the shell, and with the magnetosheath, for making the magnetosheath rotate.
2. dehydration device according to claim 1, which is characterized in that further include magnetosheath connection ring；
The magnetosheath connection ring is arranged on the housing, and one end is socketed with the magnetosheath；The other end and the electronic dress
Set connection.
3. dehydration device according to claim 1, which is characterized in that further include returning device；The returning device setting
In the shell；And for making the main shaft push rod be moved to the direction far from the magnetosheath.
4. dehydration device according to claim 1 or 2, which is characterized in that be additionally provided with circuit board in the shell；
The electric device and the circuit board electrical connection are additionally provided with the motor for protruding from the shell on the circuit board
Adjusting knob and charge port；
The button switch for control circuit board power supply is additionally provided on the shell.
5. dehydration device according to claim 4, which is characterized in that the one end open of the magnetosheath, other end closing, and
The cross-sectional area of the magnetosheath is tapered along the direction from open end to blind end.
6. dehydration device according to claim 5, which is characterized in that be provided on the outer wall of described closed one end of magnetosheath
Multiple protrusions.
7. dehydration device according to claim 6, which is characterized in that the number of the protrusion is even number, and along institute
The direction for stating magnetosheath axis is symmetrical arranged.
8. dehydration device according to claim 7, which is characterized in that the phase the distance between per two adjacent protrusions
Deng.
9. utilizing the method for claim 1-8 any one of them dehydration devices dehydration, which is characterized in that include the following steps：
1) main shaft push rod, is pushed, so that the magnetic pole is reached the blind end of the magnetosheath, then the magnetosheath of dehydration device is stretched into and is contained
It is magnetic in the solid-liquid mixture of substance；
2), magnetisable material is adsorbed to the blind end of magnetosheath under the action of the magnetic pole, then by the dehydration device from admittedly
It is removed in liquid mixture；
3) electric device, is controlled so that magnetosheath rotates, and magnetisable material is kept absorbed state, magnetic bead table by magnetic fields
Face contained humidity is got rid of de- under the influence of centrifugal force.
10. according to the method described in claim 9, it is characterized in that, in step 1), the solid-liquid mixture includes containing magnetic
The DNA lysates or eluent of pearl.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610033982.3A CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
| PCT/CA2016/000204 WO2017139864A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| CA3008660A CA3008660A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| EP16890137.9A EP3405288A4 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| US16/071,038 US20190022667A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610033982.3A CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105543090A CN105543090A (en) | 2016-05-04 |
| CN105543090B true CN105543090B (en) | 2018-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610033982.3A Expired - Fee Related CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI932866A0 (en) * | 1993-06-21 | 1993-06-21 | Labsystems Oy | Separeringsfoerfarande |
| CN200945454Y (en) * | 2006-06-16 | 2007-09-12 | 复旦大学 | Portable magnetic separating pen |
| CN104344985B (en) * | 2013-08-09 | 2018-04-03 | 英芮诚生化科技（上海）有限公司 | A kind of magnetic separation device |
| CN104646176B (en) * | 2015-02-11 | 2017-03-01 | 英芮诚生化科技（上海）有限公司 | Manual magnetic solid extractor |
| CN105062888B (en) * | 2015-09-02 | 2017-09-15 | 苏州东胜兴业科学仪器有限公司 | Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid |
| CN205473812U (en) * | 2016-01-19 | 2016-08-17 | 山西振东基赛生物科技有限公司 | Dewatering device |
-
2016
- 2016-01-19 CN CN201610033982.3A patent/CN105543090B/en not_active Expired - Fee Related
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| CN105543090A (en) | 2016-05-04 |
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