CN105543090A - Dehydration device and method - Google Patents

Dehydration device and method Download PDF

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Publication number
CN105543090A
CN105543090A CN 201610033982 CN201610033982A CN105543090A CN 105543090 A CN105543090 A CN 105543090A CN 201610033982 CN201610033982 CN 201610033982 CN 201610033982 A CN201610033982 A CN 201610033982A CN 105543090 A CN105543090 A CN 105543090A
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magnetic
sleeve
housing
end
magnetic sleeve
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CN 201610033982
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Chinese (zh)
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杨俊森
杨贵生
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山西振东基赛生物科技有限公司
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

The invention provides a dehydration device and a dehydration method, and belongs to the technical field of biologics. The dehydration device comprises a shell, a spindle push rod, a magnetic sleeve and an electric device, wherein the spindle push rod penetrates through the shell; two ends of the spindle push rod protrude outside the shell; the spindle push rod can reciprocate along the shell; a magnetic column is arranged at one end of the spindle push rod; the magnetic column is sleeved by the magnetic sleeve; the magnetic column is coaxial with the magnetic sleeve and is positioned in the center of the magnetic sleeve; one end of the opening of the magnetic sleeve is connected with the shell; the electric device is arranged inside the shell and is connected with the magnetic sleeve so as to drive the magnetic sleeve to rotate. As the centripetal force of the magnetic column and the centrifugal force generated when the magnetic sleeve is rotated mutually act, separation of magnetic beads from a liquid on the surface of the magnetic beads can be achieved, and the dehydration device is simple, convenient and rapid in dehydration mode when compared with an ordinary drying and blowing manner, and separated substances are not damaged.

Description

一种脱水装置及方法 An apparatus and method for dewatering

技术领域 FIELD

[0001]本发明涉及生物技术领域,具体而言,涉及一种脱水装置及方法。 [0001] The present invention relates to the field of biotechnology, in particular, to an apparatus and method for dewatering.

背景技术 Background technique

[0002]在生物技术领域中,脱水是生物物质分离过程中很重要但又很难解决的一道难题。 [0002] In the field of biotechnology, dehydrated biological material separation process is very important but very difficult to solve a difficult problem. 脱水之所以被称为“难题”是由于生物物质分离过程多半是在液体存在的条件下进行的;如从血液中分离淋巴细胞、癌细胞;从牛奶中分离某种细菌、病毒、或其他有害物质等等。 Dehydration is called "problems" due to biomass separation is mostly carried out in the presence of liquid conditions; lymphocytes isolated from the blood of cancer cells; separating certain bacteria, viruses from milk, or other deleterious materials and so on. 分离出来的物质总会或多或少带有从上游液体中残留的不必要的液态物质;而为了去除这些多余液体物质,常规实验室方法多采用高温烘干、室温挥发、延时等待、空气流动(风吹)等后处理手段,显然会造成费时、费事以及操作繁琐等缺陷。 Always more or less separated material with unnecessary substances remaining liquid from an upstream liquid; the excess liquid in order to remove these materials, conventional laboratory method to use high-temperature drying, volatilization temperature, wait delay, the air flow (wind) and other post-processing tools, will obviously result in time-consuming, cumbersome and complicated operation and other defects.

[0003]例如,在利用磁珠法提取核酸的过程中,裂解液将细胞裂解,磁珠将释放的核酸吸附后,需要将磁珠从裂解液中分离;而目前现有技术中常规的做法一般都是通过一个外加的磁场使得磁珠聚集于容器(装有裂解液以及磁珠,一般为离心管)的一侧;然后再利用移液枪将裂解液吸除,从而实现磁珠与裂解液的分离;被分离的磁珠进一步地经过洗脱,从而实现目的核酸的提取。 [0003] For example, after the lysate cells were lysed, releasing the adsorption of nucleic acids to magnetic beads, the magnetic beads needs to be separated from the lysate using magnetic beads in the process of extracting the nucleic acid in the method; and is currently normal practice in the prior art usually by an applied magnetic field such that the beads gathered in the container (with beads and lysate, typically centrifuge tube) side; then using a pipette gun lysate gettering magnetic beads to achieve lysis separating liquid; a further separate magnetic beads after elution of the nucleic acid to achieve extraction.

[0004]然而,该提取方法中,将裂解液和吸附有核酸的磁珠分离的过程中,聚集的磁珠中会残留有大量的液体;因此,固液分离不彻底,从而降低后续所提取的核酸的纯度。 [0004] However, the extraction method, and the lysate beads adsorbed with an isolated nucleic acid process, the aggregation of magnetic beads may remain in a large amount of liquid; therefore, solid-liquid separation is not complete, thereby reducing the subsequent extracted the purity of the nucleic acid. 若对磁珠采用空气流动(风吹)、滤纸吸附等进一步地脱水操作,一方面费时、费力,操作不便;另一方面,还可能会导致所吸附的核酸被破坏或者流失。 When using beads of air flow (wind), paper adsorption Further dehydration operation, on the one hand consuming, laborious, inconvenient operation; on the other hand, it may also cause the adsorbed nucleic acids are damaged or lost.

[0005]有鉴于此,特提出本发明。 [0005] In view of this, the present invention proposed Japanese.

发明内容 SUMMARY

[0006]本发明的第一目的在于提供一种脱水装置,以解决现有的生物物质分离技术中含有被分离物质的磁珠其固液分离不彻底(被分离物质中含有难以清除的液体的技术难题),且脱水操作费时、费力的技术问题,该装置通过将磁柱的向心力和与磁套发生旋转时产生的离心力相结合,从而实现磁珠与磁珠表面所含水分的脱离,比一般的干燥、风吹等脱水方式简便、快捷,而且不会对被分离的物质造成破坏。 [0006] The first object of the present invention is to provide a dewatering device, to solve the conventional art biomass separation beads containing substances to be separated which is not completely solid-liquid separation (separation of substances contained in a liquid to be difficult to remove the technical problems), and the dehydration operation is time-consuming, laborious technical problems, which means a centrifugal force generated by the rotation of the cylinders occurs and the centripetal force and the magnetic sleeve combination, thereby realizing the magnetic beads from the water contained in the bead surface, than easy generally dry, wind and dehydration way, fast, and will not cause damage to the material to be separated.

[0007]本发明的第二目的在于提供一种利用所述的脱水装置脱水的方法,该方法操作简便,脱水效率高,耗时短。 The second object of the [0007] present invention is to provide a method for dewatering apparatus according to the dehydration use, the method is simple, high dewatering efficiency, less time consuming.

[0008]为了实现本发明的上述目的,特采用以下技术方案: [0008] To achieve the above object of the present invention, especially the following technical solutions:

[0009]本发明提供了一种脱水装置,所述脱水装置包括壳体、主轴推杆、磁套和电动装置; [0009] The present invention provides a dewatering apparatus, a dewatering device comprises a housing, a plunger shaft, and an electric magnetic sleeve means;

[0010]所述主轴推杆贯穿所述壳体,其两端均突出于所述壳体;且所述主轴推杆能够沿着壳体往复运动;所述主轴推杆的一端设置有磁柱;所述磁套罩套于所述磁柱上,且磁柱与所述磁套同轴设置,并处于所述磁套的中心,所述磁套开口的一端连接在所述壳体上;所述电动装置设置在所述壳体内,并且与所述磁套连接,用于使所述磁套发生转动。 [0010] The push rod extending through the spindle housing, which protrudes from both ends of said housing; and the ram shaft reciprocally movable along the housing; end of the spindle is provided with a pusher cylinders ; said magnetic shroud on said sleeve cylinders, and cylinders provided coaxially with the magnetic sleeve, and the sleeve is in the magnetic center of the magnet sleeve open at one end connected to the housing; the electric device disposed within the housing and connected with the magnetic sleeve, a magnetic sleeve for rotation occurs.

[0011]对于上述的装置,其要点在于磁柱产生的磁性向心力和磁套旋转产生的离心力的相互作用,所以磁柱和磁套的位置关系很重要。 [0011] With the above apparatus, the point that the resulting magnetic interaction cylinders centripetal force and the centrifugal force generated by rotation of the magnetic sleeve, so that the positional relationship between the magnetic cylinders and sleeves are important. 该装置将磁柱设置在所述磁套的中心位置(二者同轴设置,且无论磁柱是否伸入磁套的封闭端与否,磁柱一直处于磁套的中心位置),进而对磁套封闭端的磁珠提供一个磁性向心力,当向心力和磁套旋转时产生的离心力达到某一平衡点时,磁珠受向心力的作用停留在磁套封闭端,液体受离心力作用被甩脱,从而达到脱水的效果。 The apparatus of cylinders arranged in a central position of the magnetic sleeve (two coaxially arranged cylinders and regardless of whether the sleeve extending into the closed end of the magnetic or not, has been at the center position of the magnetic cylinders sleeve), and thus the magnetic beads provide a closed end of the sleeve magnetic centripetal force, when the centrifugal force and centripetal force generated by the magnetic sleeve rotation reaches a point of equilibrium, the magnetic beads by the centripetal force acting on the magnetic sleeve stays closed end, is to shake off the liquid by centrifugal force, so as to achieve the effect of dehydration.

[0012]具体的,本发明提供的这种脱水装置,其主体结构为一个壳体,壳体上有一个将其贯穿并且可以沿着壳体往复运动的主轴推杆;在外力的作用下,主轴推杆上设置的磁柱可以伸入到磁套的底部(通过额外设置的回位装置,可使得磁柱进一步返回,从而实现主轴推杆以及磁珠的往返运动);而设置在壳体内的电动装置则可以使磁套发生旋转运动。 [0012] Specifically, the present invention provides such a dewatering device, the main structure of a housing, through which there is a push rod and can be reciprocated along the spindle housing on the housing; the external force, pusher cylinders can be provided on the spindle extending into the bottom of the magnetic sleeve (additionally provided by means of return, such cylinders may be returned further to achieve reciprocating movement of the spindle and the push rod beads); and disposed within the housing electric apparatus may occur that the rotational movement of the magnetic sleeve.

[0013]在将该脱水装置进行应用的过程中,如将其用于分离磁珠裂解液(含有吸附了核酸的磁珠以及裂解液);将磁套伸入到混合体系中,此时推压主轴推杆,使得磁珠的端部伸入到了磁套的底部;在磁珠的作用下,混合体系中的磁珠被吸附到了磁套上,然后移除,从而实现磁珠与混合体系的快速分离。 [0013] The process applied in the dewatering device, such as a lysate which was used magnetic beads (magnetic beads containing adsorbed nucleic acids and lysate) separation; magnetic sleeve extending into the mixing system, this time to push pressure plunger shaft, such that the end beads projecting into the bottom magnetic sleeve; under the action of beads, the beads mixed system is adsorbed to the magnetic sleeve is then removed, and the beads in order to achieve mixing system the rapid separation. 进一步地,由于磁套在电动装置的带动下是可以转动的,这样被吸附的磁珠在转动的过程中实现了类似离心脱水的操作(可以通过控制磁套的转速实现离心力的调节),从而使得磁珠内部所含的液相进一步地被分离。 Further, since the electric driven magnetic sleeve means is rotatable, such beads adsorbed centrifugal dewatering to achieve a similar operation during the rotation (centrifugal force adjustment can be achieved by controlling the rotational speed of the magnetic sleeve), whereby such that the liquid contained in the interior of the beads to be further separated. 离心完成后,去掉施加于主轴推杆的力,主轴推杆在回位装置的作用下回位,带动磁柱从磁套的底部离开;被吸附的磁珠由于失去了磁力的吸附作用从而从上磁套脱离,并可进行下一步的操作。 After centrifugation is complete, remove the force applied to the shaft of the push rod, pusher spindle bit next time action of the return means, to drive away from the bottom cylinders magnetic sleeve; adsorbed beads because of the loss of a magnetic force so that adsorption from from the magnetic sleeve, and to the next step.

[0014]可见,本发明提供的这种脱水装置,其使得脱水过程大大简化,大大缩短了脱水的时间,操作熟练的情况下,脱水过程可在数秒内完成,而且整个脱水的过程不会对被分离的物质造成损伤。 [0014] visible, the present invention provides such a dewatering device, which enables the dehydration process is greatly simplified, greatly reducing the time of dehydration, the skilled operation, the dehydration process can be completed within seconds, and the entire dewatering process will not the separated material damage. 另外,该脱水装置结构紧凑,非常便于在无菌条件下操作。 In addition, the dewatering apparatus is compact, is easy to operate under aseptic conditions.

[0015]可选的,还包括磁套连接环;所述磁套连接环设置在所述壳体上,且其一端与所述磁套套接;另一端与所述电动装置连接。 [0015] Optionally, further comprising a sleeve connected to the magnetic ring; said magnetic sleeve attachment ring disposed on the housing, and having one end connected with the magnetic condom; and the other end connected to the electric device.

[0016]在本发明中,磁套连接环起到将磁套和电动装置相连接的作用,因而更加方便地实现电动装置带动磁套旋转的效果。 [0016] In the present invention, a magnetic sleeve attachment ring functions of the magnetic sleeve and the electric device are connected, thereby more easily achieve the effect of the electric drive means to rotate the magnetic sleeve.

[0017]可选的,还包括回位装置;所述回位装置设置在所述壳体内;并用于使所述主轴推杆向远离所述磁套的方向运动。 [0017] Optionally, further comprising a return means; said return bit means is disposed within said housing; and for causing the movement of the plunger shaft direction away from the magnetic sleeve.

[0018]回位装置的效果是使得主轴推杆回位,即使得磁柱从磁套的底部离开,进而提高自动化操作的效果。 [0018] The effect of the return means is such that the spindle ram return, even if the cylinders have to leave from the bottom of the magnetic sleeve, and thus the effect of improving the automation. 对于回位装置的具体结构,优选设置成一个一端与主轴推杆固定,另一端与壳体固定的弹簧。 For the particular configuration of the return means, preferably provided with an end of a push rod fixed to the main shaft, the other end of the spring fixed to the housing. 通过回位装置以及对主轴推杆额外施加的压力,即可实现主轴推杆(附带磁柱)往返运动。 By the return of the spindle apparatus and putting additional pressure applied, can be realized putter shaft (with cylinders) back and forth motion.

[0019]可选的,所述壳体内还设置有电路板;所述电动装置与所述电路板电连接,所述电路板上还设置有均凸出于所述壳体的马达调节旋钮以及充电口;所述壳体上还设置有用于控制电路板电源的按钮开关。 [0019] Optionally, the housing is further provided with a circuit board; said motor means is electrically connected to the circuit board, said circuit board are further provided with a projection on the motor housing and the adjusting knob charging port; said upper housing further provided with a button switch for controlling a power supply circuit board.

[0020]电路板可集成各种线路,通过马达调节旋钮,可实现电动装置的输出功率的调节,并直接控制了磁套连接环以及磁套的转动速率;实现磁套的转速调节;而通过充电口(为方便起见,可设置成USB接口),可以给电源(内设于壳体)实现充电效果;此外,壳体上还设置有用于控制电路板电源的按钮开关。 [0020] The circuit board may integrate the various lines, by a motor adjusting knob to adjust the output power of the electric apparatus can be realized, and directly controls the magnetic ring and a sleeve connected to the rate of rotation of the magnetic sleeve; rotational speed control to achieve a magnetic sleeve; through charging port (for convenience, the USB interface may be arranged), it may be to the power (in case equipped) for charge effect; in addition, provided with a button switch for controlling the power supply circuit board on the housing.

[0021]可选的,所述磁套的一端开口,另一端封闭,且所述磁套的横截面积沿从开口端到封闭端的方向渐缩。 [0021] Optionally, open end of the magnetic sleeve, the other end is closed, and the sleeve of the magnetic cross-sectional area in a direction from the open end to the closed end of the taper.

[0022]在脱水的过程中,磁套会伸入到混合液体系中,其外壁会挂有水滴。 [0022] In the dehydration process, the magnetic sleeve will extend into the mixture system, the outer wall will be hanging drop. 因此,为了提高脱水的效果,优选地将磁套的横截面积设置成沿从开口端到封闭端的方向渐缩的形式,在转动离心的过程中,具有该结构的磁套便于所粘附的水自上而下脱离,从而提高脱水效率。 Therefore, in order to improve the dewatering effect, it is preferable to set the magnetic cross-sectional area is arranged in a direction from the open end to the closed end of the tapered form, the rotation of the centrifugal process, the sleeve having a magnetic structure facilitates the adhered water from top to bottom, thereby improving the efficiency of dehydration.

[0023]可选的,所述磁套封闭的一端的外壁上设置有多个凸起。 [0023] Alternatively, the magnetic wall of the outer sleeve closed at one end is provided with a plurality of projections.

[0024]可选的,述凸起的数目为偶数个,且沿着所述磁套轴线的方向对称设置。 [0024] Alternatively, the number of said projections is an even number, and symmetrically disposed along a direction of the magnetic axis of the sleeve.

[0025]可旋的,每相邻的两个所述凸起之间的距离相等。 [0025] The rotatable, equal to the distance between each adjacent two of said projections.

[0026]此外,为了增加磁套在转动过程中的研磨效果,优选地,将磁套的外表面设置成粗面。 [0026] Further, in order to increase the abrasive effect of magnetic sleeve during rotation, preferably, the outer surface of the magnetic sleeve is provided to a coarse surface. 进一步优选的,为了提高磁套在转动过程中的搅拌效果,可以在磁套的盲端设置有一字型或者十字型的小型风叶。 Further preferably, in order to improve the stirring effect magnetic sleeve during rotation may be provided with a cross-shaped or a fan-type mini blind end of the magnetic sleeve.

[0027]所述的脱水装置脱水的方法,包括如下步骤: [0027] The dewatering apparatus according to the dehydration method, comprising the steps of:

[0028] 1)、推动主轴推杆,使所述磁柱到达所述磁套的封闭端,然后将脱水装置的磁套伸入含有磁磁性物质的固液混合体中; [0028] 1), the push rod advancing spindle, said cylinders reach the closed end of the magnetic sleeve, and the sleeve magnetic dewatering device extending into the solid-liquid mixture containing a magnetic substance of a magnetic body;

[0029] 2)、磁性物质在所述磁柱的作用下被吸附到磁套的封闭端,然后将所述脱水装置从固液混合体中移除; [0029] 2), the magnetic substance is adsorbed under the action of the cylinders to the closed end of the magnetic sleeve, and then the device is removed from the dehydration of the solid-liquid mixture;

[0030] 3)、控制电动装置,使得磁套发生旋转,磁性物质受磁场作用保持被吸附状态,磁珠表面所含水分在离心力的作用下被甩脱。 [0030] 3), electric control means, so that the magnetic sleeve rotation occurs, the magnetic substance by the magnetic field remains adsorbed state, moisture is contained in the surface of the beads under the action of centrifugal force purging.

[0031]该脱水方法采用特制的脱水装置完成,对于含有磁性物质的固液混合体而言,其可以在数秒之间实现磁珠和磁珠表面液体的的分离,而且对被分离的物质不会造成损伤,大大地节约了时间,并且提高了效率。 [0031] The dehydration process is completed using a special dewatering device for containing the magnetic substance to solid-liquid mixture, its possible separation beads and bead between the surface of the liquid in a few seconds, but not the separated substance cause damage, greatly save time and improve efficiency.

[0032]可选的,在步骤I)中,所述固液混合体包括含有磁珠的DNA裂解液或者洗脱液。 [0032] Optionally, in step I), the solid-liquid mixture comprising DNA-containing eluate lysate or beads.

[0033]对于包括含有磁珠的DNA裂解液或者洗脱液,其非常适合作为该脱水装置的处理对象,磁珠可在很短的时间内被全部从混合体现中吸附并移除,旋转离心的过程也一般可在数秒之间完成,释放磁力后,磁珠可进行下一步操作进程,洗脱后再次将磁珠分离,即可得到目的核酸,而磁珠经过离心脱水后另行存放或者再次利用。 [0033] For an eluent comprising DNA containing lysate or beads, which is very suitable for the objects in the dewatering device, all the beads may be adsorbed and removed from the mixed embodied in a very short period of time, the rotation centrifugal the process is generally completed in a matter of seconds, after releasing the magnetic force, magnetic beads can progress to the next step, the beads after elution again isolated, the nucleic acid can be obtained, and the magnetic beads after the centrifugal dehydration in storage, or again use.

[0034]利用上述的装置,本发明可解决现有该领域中无法解决的难题。 [0034] With the above apparatus, the present invention can solve the prior art can not solve the problem. 当利用纳米磁珠分离生物物质时,推动本装置的主轴推杆,所述磁柱即可到达所述磁套的封闭端提供磁场,此时如将所述磁套伸入到含有磁珠的混合液体中,磁珠即可被吸附在所述磁套的封闭端外侧;由于所述磁柱位于所述磁套的中心位置,对于磁珠来说,所述磁柱提供的是一个磁性向心力。 When using magnetic bead separation of the biomass, this means pushing the plunger of the spindle, said cylinders to reach the closed end of the sleeve to provide a magnetic field, as will be the case projects into the sleeve containing the magnetic beads mixed liquid, the magnetic beads are adsorbed to the outside of the closed end of the magnetic sleeve; since the cylinders located in the center of the magnetic sleeve, for magnetic beads, the said cylinders is provided a magnetic centripetal . 如将所述磁套的封闭端提出液面,并启动旋转按钮,使磁套产生旋转运动,此时的旋转运动对所述磁珠和其表面的液体将产生离心力。 As the closed end of the magnetic sleeve made level, and start a spin button, a rotational movement of the magnetic sleeve to produce, in this case the rotational movement of the beads and the liquid surface which will generate a centrifugal force. 用于磁珠在磁场的向心力作用下保持原位,液体则不受磁力的影响而被离心力甩脱,这个离心力和向心力的相互作用使磁珠和磁珠表面液体起到了分离效果。 Magnetic beads used in the centripetal force holding the magnetic field in situ, the liquid is not affected by magnetic force and to shake off by centrifugal force, the centrifugal and centripetal forces of interaction between the magnetic beads and the bead surface liquid separation played. 整个脱水过程可在数秒内完成。 Dewatering the whole process can be completed in seconds.

[0035]与现有技术相比,本发明的有益效果为: [0035] Compared with the prior art, the beneficial effects of the present invention are:

[0036] (I)、该装置通过将磁柱的磁力和与磁套发生旋转时产生的离心力相结合,从而实现磁珠所含水分的脱离,比一般的干燥、风吹等脱水方式简便、快捷,而且不会对被分离的物质造成破坏。 Simple and [0036] (I), which means a centrifugal force generated by the rotation of the cylinders occurs and the magnetic force and sleeve combination, in order to achieve the moisture contained departing from the beads, than the average drying, dehydration wind mode, fast, and will not cause damage to the material to be separated.

[0037] (2)、由于该脱水装置结构紧凑便携,因此非常适合作为野外试验的实验工具,同时也非常便于在无菌条件下进行操作。 [0037] (2), since the dewatering device is compact and portable, and therefore well suited as a tool for experimental field trials, but also very easy to be operated under sterile conditions.

[0038] (3)、对磁套的外表面进行进一步的改进(如设置成粗面、增加风叶等)可使得该脱水装置实现研磨、搅拌装置的效果,具有多功能性,可作为生物学领域中一种非常适用的实验工具。 [0038] (3), on the outer surface of the magnetic sleeve further improvements (e.g., set as a rough surface to increase the fan, etc.) can be implemented so that the grinding dewatering device, the effect of the stirring device having versatility can be used as a biological a very applicable in the field of experimental tools.

附图说明 BRIEF DESCRIPTION

[0039]为了更清楚地说明本发明实施例或现有技术中的技术方案,以下将对实施例或现有技术描述中所需要使用的附图作简单地介绍。 [0039] In order to more clearly illustrate the technical solutions in the embodiments or the prior art embodiment of the present invention, the following embodiments or the accompanying drawings required for describing the prior art will be used in the embodiments are introduced briefly.

[0040]图1为本发明提供的优选方案中的脱水装置的结构示意图; [0040] FIG. 1 is a schematic structural diagram dewatering device embodiment of the present invention is preferably provided in;

[0041]图2为本发明的另一技术方案中的脱水装置的结构示意图; [0041] FIG. 2 is a schematic structural diagram of another aspect of the dewatering device of the present invention;

[0042]附图标记: [0042] The reference numerals:

[0043] 壳体-101;主轴推杆-102;磁套-103;电动装置_104;磁柱-105;磁套连接环-201;回位装置-202 ;按钮开关-203 ;凸起-204。 [0043] The housing -101; -102 ram shaft; Magnetosheath -103; electric means _104; cylinders -105; -201 magnetic sleeve connecting ring; means the return -202; -203 button switch; projection - 204.

具体实施方式 detailed description

[0044]请参考图1-图2,本发明提供的这种脱水装置,其包括壳体101、主轴推杆102、磁套103和电动装置104; [0044] Please refer to FIGS. 1-2, the present invention provides such a dewatering device, which comprises a housing 101, a plunger shaft 102, magnet 103 and sleeve 104 electrically powered device;

[0045]主轴推杆102贯穿壳体101,其两端均突出于壳体101;且主轴推杆102能够沿着壳体101往复运动;主轴推杆102的一端设置有磁柱105;磁套103罩套于磁柱105上(具体如图2所示),且其开口的一端连接在壳体101上;电动装置104设置在壳体101内,并且与磁套103连接,用于使磁套103发生转动。 [0045] The shaft housing 101 through the push rod 102, which protrudes from both ends of the housing 101; spindle and the push rod 102 can reciprocate along the housing 101; an end of the push rod 102 of the spindle 105 is provided with a magnetic column; Magnetosheath One end of the cover sleeve 103 on the magnetic column 105 (specifically shown in Figure 2), and which is connected to an opening in the housing 101; 104 electrical device disposed within the housing 101, and is connected to the magnetic sleeve 103, a magnetic sleeve 103 from rotating.

[0046]本发明提供的这种脱水装置,其主体结构为一个壳体101,壳体101上有一个将其贯穿并且可以沿着壳体101往复运动的主轴推杆102;在外力的作用下,主轴推杆102上设置的磁柱105可以伸入到磁套103的底部;而设置在壳体101内的电动装置104则可以使磁套103发生转动。 [0046] The present invention provides such a dewatering device, the main structure of a housing 101, which has a through upper housing 101 and pusher 102 can reciprocate along the major axis 101 of the housing; the action of external forces under , provided on the spindle plunger cylinders 102,105 may extend to the bottom of the magnetic sleeve 103; and 101 disposed within the housing 104 can be an electric device 103 rotates with the magnetic sleeve.

[0047]当将该生物分离装置进行应用的过程中,如将其用于分离磁珠裂解液(含有吸附了核酸的磁珠以及裂解液);将磁套103伸入到混合体系中,此时推压主轴推杆102,使得磁柱105的端部伸入到了磁套103的底部;在磁柱105的作用下,混合体系中的磁柱105被吸附到了磁套103上,然后移除,从而实现磁珠与混合体系的快速分离。 [0047] When the process of bio-separation device applications, such as magnetic beads for separating the lysate (containing the nucleic acid adsorbed beads and lysate); the magnetic sleeve 103 projects into the mixing system, this when pushing the plunger shaft 102, so that the ends of the cylinders 105 extending into the bottom 103 of the magnetic sleeve; under the action of cylinders 105, the mixed system of the cylinders 105 is adsorbed to the magnetic sleeve 103 is then removed in order to achieve rapid separation beads and mixed systems. 进一步地,由于磁套103在电动装置104的带动下是可以转动的,附在磁套103封闭端外侧的磁珠在磁力的作用下保留在磁套103上,而磁珠表面的液体则在离心力的作用下与磁珠分离。 Further, since the magnetic sleeve 103 driven by motor means 104 is rotatably attached on the outer sleeve 103 closed end of the magnetic beads retained on the magnetic sleeve 103 under the influence of magnetic force, while the liquid at the surface of the beads separating the beads by the centrifugal force.

[0048]离心完成后,去掉施加于主轴推杆102的力,主轴推杆102在回位装置202的作用下回位,带动磁柱105从磁套103的底部离开;被吸附的磁珠由于失去了磁力的吸附作用从而从上磁套103脱离,并可非常便利地进行下一步的操作。 [0048] After the completion of centrifugation, removing the force applied to the push rod of the spindle 102, the spindle effect the next time the push rod 102 in the position of the return means 202, 105 away from the bottom of the drive cylinders 103 of the magnetic sleeve; since the adsorbed beads lost magnetic adsorption to disengage from the magnetic sleeve 103, and very conveniently to the next step.

[0049]可见,本发明提供的这种脱水装置,其使得脱水过程大大简化,大大缩短了脱水的时间,脱水过程可在数秒内完成,而且整个脱水的过程不会对被分离的物质造成损伤。 [0049] As can be seen, the present invention provides such a dewatering device, which enables the dehydration process is greatly simplified, greatly reducing the time of dehydration, the dehydration process can be completed within a few seconds, and the entire dewatering process will not damage the substance to be separated . 另外,该脱水装置结构紧凑,非常便于在无菌条件下操作。 In addition, the dewatering apparatus is compact, is easy to operate under aseptic conditions.

[0050] 在上述技术方案的基础之上,进一步优选的,还包括磁套连接环201;磁套连接环201设置在壳体101上,且其一端与磁套103套接;另一端与电动装置104连接(也即磁套103通过磁套连接环201与电动装置104实现连接,电动装置104通过直接驱动磁套连接环201实现使磁套103发生旋转运动)。 [0050] Based on the foregoing technical solution above, further preferred, further comprising a sleeve connected to the magnetic ring 201; magnetic sleeve attachment ring 201 is provided on the housing 101, one end of the magnetic sleeve and the socket 103; the other end of the electric connecting means 104 (i.e. the magnetic sleeve 103 by a magnetic ring 201 connected to sleeve 104 to achieve the electric connection means, electric means driving the magnetic sleeve 104 by directly connecting the magnetic ring 201 to achieve rotational movement of the sleeve 103 occurs).

[0051 ] 磁套连接环201起到将磁套103和电动装置104相连接的作用,因而更加方便地实现电动装置104带动磁套103旋转的效果。 [0051] The magnetic yoke attachment ring 201 functions as the magnetic sleeve 103 and the electric device 104 is connected, and therefore more easily achieve the electric drive means 104 effects rotation of the magnetic sleeve 103.

[0052]在生物学实验中,对于各种操作设备,其自动化程度的高低直接影响了整个实验的成败以及效率;因此,为了提高该装置的自动化性能,在上述方案的基础之上,进一步优选的,还包括回位装置202;回位装置202设置在壳体101内;并用于使主轴推杆102向远离磁套103的方向运动;壳体1I内还设置有电路板;电动装置104与电路板电连接,电路板上还设置有均凸出于壳体101的马达调节旋钮以及充电口;壳体101上还设置有用于控制电路板电源的按钮开关203。 [0052] In the biological experiments, for the various operating devices, which directly affects the level of automation of the success and the efficiency of the experiment; thus, in order to improve the performance of the automatic apparatus, on the basis of the above-described embodiment, and further preferably further comprising a return means 202; 202 return means disposed within the housing 101; and the push rod 102 for the spindle in a direction away from the sleeve 103 of the magnetic motion; 1I is also provided a circuit board inside the housing; an electric device 104 and is electrically connected to the circuit board, the circuit board is also provided with a motor housing 101 are protruding from the knob, and a charging port; button switch is also provided for controlling the power supply circuit board 203 of the housing 101.

[0053]回位装置202的效果是使得主轴推杆102回位,即使得磁柱105从磁套103的底部离开,进而提高自动化操作的效果。 [0053] The effect of the return means 202 is such that the spindle return the push rod 102, even have the bottom of the magnetic cylinders 105 away from the sleeve 103, thereby increasing the effect of automation. 通过回位装置202以及额外施加于主轴推杆102的力,即可实现主轴推杆102以及磁柱105顺着壳体101往复运动。 By the return means 202 and an additional force applied to the push rod 102 of the spindle, the spindle can be realized ram cylinders 102 and 105 along housing 101 to reciprocate.

[0054]对于回位装置202的具体结构,优选设置成一个一端与主轴推杆102固定,另一端与壳体101固定的弹簧。 [0054] For the return of the specific configuration of the device 202, preferably provided with a spindle end of the push rod 102 is fixed, the other end of the spring fixed to the housing 101.

[0055]另外,其也可以是一个套设在主轴推杆102上的弹簧,一端通过连接件与主轴推杆102固定,另一端与壳体101固定。 [0055] Further, it may be a sleeve disposed in the spindle plunger spring 102, one end of the push rod 102 is fixed, and the other end fixed to the housing 101 by a connecting member to the spindle.

[0056]电路板、马达调节旋钮以及按钮开关203可以控制整个脱水装置的工作状态,进一步地方便了使用者使用。 [0056] the circuit board, the motor adjustment knob and a push button switch 203 may control the operating state of the overall dewatering device further facilitates the user to use.

[0057]在本发明提供的另一优选的技术方案中,脱水装置包括:壳体101、主轴推杆102、磁套103和电动装置104、磁套连接环201、回位装置202; [0057] In another preferred aspect of the present invention provides the dewatering apparatus comprising: a housing 101, a plunger shaft 102, magnet 103 and sleeve 104 electrically powered device, a magnetic yoke attachment ring 201, the return means 202;

[0058]主轴推杆102贯穿壳体101,其两端均突出于壳体101;且主轴推杆102能够沿着壳体101往复运动;主轴推杆102的一端设置有磁柱105;磁套103罩套于磁柱105上,且其开口的一端连接在壳体101上;电动装置104设置在壳体101内,并且与磁套103连接,用于使磁套103发生转动; [0058] The shaft housing 101 through the push rod 102, which protrudes from both ends of the housing 101; spindle and the push rod 102 can reciprocate along the housing 101; an end of the push rod 102 of the spindle 105 is provided with a magnetic column; Magnetosheath One end of the jacket 103 to the cylinders 105, and it is connected to an opening in the housing 101; 104 motor means disposed within the housing 101, and is connected to the magnetic sleeve 103, a sleeve 103 rotates with the magnetic;

[0059]磁套连接环201设置在壳体101上,且其一端与磁套103套接;另一端与电动装置104连接;回位装置202设置在壳体101内;并用于使主轴推杆102向远离磁套103的方向运动; [0059] The magnetic yoke attachment ring 201 is provided on the housing 101, one end of the magnetic sleeve and the socket 103; the other end connected to the electric device 104; return means 202 disposed within the housing 101; and for the pusher shaft 102 motion to the sleeve 103 in a direction away from the magnet;

[0060]壳体1I内还设置有电路板;电动装置104与电路板电连接,电路板上还设置有均凸出于壳体101的马达调节旋钮以及充电口;壳体101上还设置有用于控制电路板电源的按钮开关203; [0060] 1I provided a circuit board within the housing; an electric device 104 is electrically connected to the circuit board, the circuit board are provided with a projection on the motor housing 101 and the adjusting knob charging port; provided on the housing 101 is useful button on the control circuit board of the power switch 203;

[0061] 磁套103的一端开口,另一端封闭,且磁套103的横截面积沿从开口端到封闭端的方向渐缩;磁套103封闭的一端的外壁上设置有多个凸起204;凸起204的数目为偶数个,且沿着磁套103轴线的方向对称设置;每相邻的两个凸起204之间的距离相等。 End of [0061] magnetic sleeve opening 103, the other end is closed, and the cross sectional area of ​​the magnetic sleeve 103 along a direction from the open end to the closed end of the tapered; magnetic sleeve closed at one end 103 of the outer wall is provided with a plurality of projections 204; the number is an even number of projections 204 and 103 symmetrically disposed in the direction of the axis of the magnetic sleeve; each distance between two adjacent projections 204 are equal.

[0062]具有上述结构的脱水装置,其可以集脱水、搅拌、涡旋等多功能为一体,满足多种生物实验的需要。 [0062] dewatering device having the above structure, it may be set dehydration, stirring, vortexing other functions as one, to meet the various needs of biological experiments. 另外如果有必要使得该装置具有研磨功能,则对应地将该磁套103的外表面设置成粗面即可,如果要实现更好的搅拌效果,则在磁套103的盲端增设一字型或者十字型的小型风叶。 Also, if it is necessary that the device has a grinding function, corresponds to the outer surface of the magnetic sleeve 103 is set as a rough surface can be, if you want to achieve better stirring effect, the addition of a font in the blind end of the magnetic sleeve 103 or a small cross-shaped blades.

[0063]本发明还提供了一种利用该脱水装置脱水的方法,具体包括以下步骤: [0063] The present invention further provides a method of use of the dewatering device dehydration, includes the following steps:

[0064] S1、推动主轴推杆102,使所述磁柱105到达所述磁套103的封闭端,然后将脱水装置的磁套103伸入含有磁磁性物质的固液混合体中; [0064] S1, the pusher advancing spindle 102, the cylinders 105 reach the closed end of the magnetic sleeve 103, and then the magnetic sleeve 103 extends into the dewatering device solid-liquid mixture containing magnetic substance in a magnetic body;

[0065]在该步骤中,固液混合体包括含有磁珠的DNA裂解液或者洗脱液。 [0065] In this step, the solid-liquid mixture containing magnetic beads comprising a DNA lysate or eluent.

[0066] S2、磁性物质在所述磁柱105的作用下被吸附到磁套103的封闭端,然后将所述脱水装置从固液混合体中移除; [0066] S2, the magnetic substance is adsorbed under the action of the cylinders 105 to the closed end of the magnetic sleeve 103, and then the device is removed from the dehydration of the solid-liquid mixture;

[0067]在该步骤中,实现了将吸附了被分离物质的磁珠直接从混合液体系中移除的效果;该方式与传统的从混合液体系中移除液相的方式相比,具有明显的优越性,首先分离后磁珠内的液相残存较少(操作过程中会在重力下自然空出);另外,克服了现有技术中将液相吸除而存在的磁珠可能损失的缺陷。 [0067] In this step, the effect achieved beads adsorbed substances to be separated is removed from the mixture directly in the system; the embodiment as compared with the conventional manner to remove the liquid phase from the mixture system, having obvious advantages, the first separated liquid phase within less residual magnetic beads (empty during operation a naturally under gravity); in addition, in the prior art to overcome the presence of a liquid phase gettering beads may be lost Defects.

[0068] S3、控制电动装置104,使得磁套103发生旋转,磁性物质受磁场作用保持被吸附状态,磁珠表面所含水分在离心力的作用下被甩脱。 [0068] S3, the electric control device 104, so that the magnetic sleeve 103 is rotated, the magnetic substance by the magnetic field remains adsorbed state, moisture is contained in the surface of the beads under the action of centrifugal force purging.

[0069]该方法基于特制的脱水装置,直接将被分离的磁珠从混合液体现中移除,从而提高了脱水的效果,另外,在磁套103转动的过程中,残存的水分可被甩出,进一步地提高了脱水的效果,而且该脱水方法利用一个装置即可完成,装置和方法相结合,使得脱水的操作在数秒内即可高效完成,脱水过程不会对被分离物质造成任何损伤;因此具有非常好的应用前景。 [0069] This method, to be separated directly on a special magnetic beads removed from the dehydration means embodied in the mixture, thereby enhancing the effect of dewatering, addition, magnetic sleeve 103 rotatable in the process, the residual moisture can be dumped a further improved effect of dehydration and the dehydration process can be completed by using a device, apparatus and method of combining, so that the operation can be dehydrated efficiently completed within a few seconds, a dehydration process will not cause any damage to the substances to be separated ; it has a very good prospect.

[0070]尽管已用具体实施例来说明和描述了本发明,然而应意识到,在不背离本发明的精神和范围的情况下可以作出许多其它的更改和修改。 [0070] Although the embodiments specifically illustrated and described by the present invention, it should be appreciated that many other changes may be made and modifications without departing from the spirit and scope of the invention. 因此,这意味着在所附权利要求中包括属于本发明范围内的所有这些变化和修改。 Thus, this means comprises in the appended claims all such changes and modifications belong to the scope of the invention.

Claims (10)

  1. 1.一种脱水装置,其特征在于,所述脱水装置包括:壳体、主轴推杆、磁套和电动装置; 所述主轴推杆贯穿所述壳体,其两端均突出于所述壳体;且所述主轴推杆能够沿着壳体往复运动;所述主轴推杆的一端设置有磁柱;所述磁套罩套于所述磁柱上,且磁柱与所述磁套同轴设置,并处于所述磁套的中心,所述磁套开口的一端连接在所述壳体上; 所述电动装置设置在所述壳体内,并且与所述磁套连接,用于使所述磁套发生转动。 A dewatering device, characterized in that said dewatering device comprising: a housing, a plunger shaft, and an electric magnetic sleeve means; said push rod extending through the spindle housing, which protrudes from both ends of the shell thereof; and the ram shaft reciprocally movable along the housing; end of the spindle is provided with a ram cylinders; said magnetic shroud on said sleeve cylinders, and cylinders with the same magnetic sleeve shaft is provided, and at the center of the magnet sleeve, one end of the magnetic sleeve opening is connected to said housing; said motor means disposed within said housing and connected with the magnetic sleeve, for the said magnetic sleeve from rotating.
  2. 2.根据权利要求1所述的脱水装置,其特征在于,还包括磁套连接环; 所述磁套连接环设置在所述壳体上,且其一端与所述磁套套接;另一端与所述电动装置连接。 2. A dewatering apparatus according to claim 1, wherein the sleeve further comprises a magnetic connecting ring; said magnetic sleeve attachment ring disposed on the housing, and having one end connected with the magnetic condom; the other end the electric device is connected.
  3. 3.根据权利要求1所述的脱水装置,其特征在于,还包括回位装置;所述回位装置设置在所述壳体内;并用于使所述主轴推杆向远离所述磁套的方向运动。 3. A dewatering apparatus according to claim 1, characterized by further comprising a return means; said return means disposed within said housing; and for causing the main magnetic direction away from the push rod sleeve movement.
  4. 4.根据权利要求1或2所述的脱水装置,其特征在于,所述壳体内还设置有电路板; 所述电动装置与所述电路板电连接,所述电路板上还设置有均凸出于所述壳体的马达调节旋钮以及充电口; 所述壳体上还设置有用于控制电路板电源的按钮开关。 The dewatering device of claim 1 or claim 2, wherein further provided a circuit board within said housing; said motor means is electrically connected to the circuit board, said circuit board is provided with a further projection are the motor housing for the adjustment knob, and a charging port; said further button switch is provided for controlling the power supply circuit board on the housing.
  5. 5.根据权利要求4所述的脱水装置,其特征在于,所述磁套的一端开口,另一端封闭,且所述磁套的横截面积沿从开口端到封闭端的方向渐缩。 The dewatering apparatus according to claim 4, characterized in that the open end of the magnetic sleeve, the other end is closed, and the sleeve of the magnetic cross-sectional area in a direction from the open end to the closed end of the taper.
  6. 6.根据权利要求5所述的脱水装置,其特征在于,所述磁套封闭的一端的外壁上设置有多个凸起。 6. A dewatering device as claimed in claim 5, wherein the magnetic wall of the outer sleeve closed at one end is provided with a plurality of projections.
  7. 7.根据权利要求6所述的脱水装置,其特征在于,所述凸起的数目为偶数个,且沿着所述磁套轴线的方向对称设置。 7. The dewatering apparatus according to claim 6, wherein the number of projections is an even number, and symmetrically disposed along a direction of the magnetic axis of the sleeve.
  8. 8.根据权利要求7所述的脱水装置,其特征在于,每相邻的两个所述凸起之间的距离相等。 8. The dewatering apparatus according to claim 7, characterized in that the equal distance between each adjacent two of said projections.
  9. 9.利用权利要求1-8任一项所述的脱水装置脱水的方法,其特征在于,包括如下步骤: 1)、推动主轴推杆,使所述磁柱到达所述磁套的封闭端,然后将脱水装置的磁套伸入含有磁磁性物质的固液混合体中; 2)、磁性物质在所述磁柱的作用下被吸附到磁套的封闭端,然后将所述脱水装置从固液混合体中移除; 3)、控制电动装置,使得磁套发生旋转,磁性物质受磁场作用保持被吸附状态,磁珠表面所含水分在离心力的作用下被甩脱。 9. A method of using a dewatering device as claimed in any dehydration of claims 1-8, characterized in that it comprises the following steps: 1), the push rod advancing spindle, reaches the closed end of the cylinders of the magnetic sleeve, the sleeve is then dehydrated magnetic means extends into the solid-liquid mixture containing magnetic magnetic substance; and 2), the magnetic substance is adsorbed magnetically to the closed end of the sleeve under the action of the cylinders, and then the solid from the dewatering device removing liquid mixture; 3), electric control means, so that the magnetic sleeve rotation occurs, the magnetic substance by the magnetic field remains adsorbed state, moisture is contained in the surface of the beads under the action of centrifugal force purging.
  10. 10.根据权利要求9所述的方法,其特征在于,在步骤I)中,所述固液混合体包括含有磁珠的DNA裂解液或者洗脱液。 10. The method according to claim 9, wherein, in step I), the solid-liquid mixture comprising DNA-containing eluate lysate or beads.
CN 201610033982 2016-01-19 2016-01-19 Dehydration device and method CN105543090A (en)

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CN 201610033982 CN105543090A (en) 2016-01-19 2016-01-19 Dehydration device and method
PCT/CA2016/000204 WO2017139864A1 (en) 2016-01-19 2016-08-05 Multifunctional biological substance separation device

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000247A1 (en) * 1993-06-21 1995-01-05 Labsystems Oy Separation method
CN200945454Y (en) * 2006-06-16 2007-09-12 复旦大学 Portable magnetic separating pen
CN104344985A (en) * 2013-08-09 2015-02-11 英芮诚生化科技(上海)有限公司 Magnetic separation device capable of sequentially extracting and continuously eluting trace substances
CN104646176A (en) * 2015-02-11 2015-05-27 英芮诚生化科技(上海)有限公司 Manual magnetic solid extractor
CN105062888A (en) * 2015-09-02 2015-11-18 苏州东胜兴业科学仪器有限公司 Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument
CN205473812U (en) * 2016-01-19 2016-08-17 山西振东基赛生物科技有限公司 Dewatering device

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995000247A1 (en) * 1993-06-21 1995-01-05 Labsystems Oy Separation method
CN200945454Y (en) * 2006-06-16 2007-09-12 复旦大学 Portable magnetic separating pen
CN104344985A (en) * 2013-08-09 2015-02-11 英芮诚生化科技(上海)有限公司 Magnetic separation device capable of sequentially extracting and continuously eluting trace substances
CN104646176A (en) * 2015-02-11 2015-05-27 英芮诚生化科技(上海)有限公司 Manual magnetic solid extractor
CN105062888A (en) * 2015-09-02 2015-11-18 苏州东胜兴业科学仪器有限公司 Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument
CN205473812U (en) * 2016-01-19 2016-08-17 山西振东基赛生物科技有限公司 Dewatering device

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