CN105543090A - Dehydration device and method - Google Patents
Dehydration device and method Download PDFInfo
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- CN105543090A CN105543090A CN201610033982.3A CN201610033982A CN105543090A CN 105543090 A CN105543090 A CN 105543090A CN 201610033982 A CN201610033982 A CN 201610033982A CN 105543090 A CN105543090 A CN 105543090A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention provides a dehydration device and a dehydration method, and belongs to the technical field of biologics. The dehydration device comprises a shell, a spindle push rod, a magnetic sleeve and an electric device, wherein the spindle push rod penetrates through the shell; two ends of the spindle push rod protrude outside the shell; the spindle push rod can reciprocate along the shell; a magnetic column is arranged at one end of the spindle push rod; the magnetic column is sleeved by the magnetic sleeve; the magnetic column is coaxial with the magnetic sleeve and is positioned in the center of the magnetic sleeve; one end of the opening of the magnetic sleeve is connected with the shell; the electric device is arranged inside the shell and is connected with the magnetic sleeve so as to drive the magnetic sleeve to rotate. As the centripetal force of the magnetic column and the centrifugal force generated when the magnetic sleeve is rotated mutually act, separation of magnetic beads from a liquid on the surface of the magnetic beads can be achieved, and the dehydration device is simple, convenient and rapid in dehydration mode when compared with an ordinary drying and blowing manner, and separated substances are not damaged.
Description
Technical field
The present invention relates to biological technical field, in particular to a kind of dewatering unit and method.
Background technology
In biological technical field, dehydration is very important in biological substance sepn process but very scabrous a difficult problem.Dehydration why be called as " difficult problem " due to biological substance sepn process be carry out under liquid existent condition mostly; As being separated lymphocyte, cancer cells from blood; Certain bacterium, virus or other objectionable impuritiess etc. is separated from milk.The material separated is always more or less with unnecessary fluent meterial residual from upstream liquid; And in order to remove these surplus liquid materials, the aftertreatment means such as the volatilization of conventional laboratory methods many employings hyperthermia drying, room temperature, time delay wait, air flowing (wind), obviously can cause the defects such as time-consuming, bothersome and complex operation.
Such as, utilizing paramagnetic particle method to extract in the process of nucleic acid, lysate is by lysis, and magnetic bead, by after the nucleic acid absorption of release, needs magnetic bead to be separated from lysate; And way conventional in currently available technology is all generally make magnetic bead be gathered in the side of container (lysate and magnetic bead are housed, are generally centrifuge tube) by an additional magnetic field; And then utilize liquid-transfering gun to be absorbed by lysate, thus realize being separated of magnetic bead and lysate; Separated magnetic bead further through wash-out, thus realizes the extraction of object nucleic acid.
But, in this extracting method, by lysate and be adsorbed with in the process of Beads enrichment of nucleic acid, in the magnetic bead of gathering, a large amount of liquid can be remained; Therefore, solid-liquid separation is not thorough, thus reduces the purity of follow-up extracted nucleic acid.If adopt the dehydration operation further such as air flowing (wind), Adsorption of Filter Paper to magnetic bead, time-consuming, effort on the one hand, operation inconvenience; On the other hand, adsorbed nucleic acid also may be caused to be destroyed or to run off.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of dewatering unit, to solve its solid-liquid separation of magnetic bead thorough (containing the technical barrier being difficult to the liquid removed in separated material) containing separated material in existing biological substance isolation technique, and dehydration operation is time-consuming, the technical problem of effort, this device is combined by the centrifugal force produced by the centripetal force of magnetic post with when rotating with magnetosheath, thus realize the disengaging of magnetic bead and magnetic bead surfaces contained humidity, than general drying, the dewatering types such as wind are easy, fast, and can not separated material be damaged.
The second object of the present invention is to provide a kind of method utilizing described dewatering unit dehydration, and the method is easy and simple to handle, and dewatering efficiency is high, consuming time short.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
The invention provides a kind of dewatering unit, described dewatering unit comprises housing, main shaft push rod, magnetosheath and electric device;
Described main shaft push rod runs through described housing, and its two ends all protrude from described housing; And described main shaft push rod can along housing to-and-fro movement; One end of described main shaft push rod is provided with magnetic post; The described magnetosheath enclosure is on described magnetic post, and magnetic post and described magnetosheath are coaxially arranged, and is in the center of described magnetosheath, and one end of described magnetosheath opening connects on the housing; Described electric device is arranged in described housing, and is connected with described magnetosheath, rotates for making described magnetosheath.
For above-mentioned device, its main points are that the magnetic centripetal force that magnetic post produces and magnetosheath rotate the interaction of the centrifugal force produced, so the position relationship of magnetic post and magnetosheath is very important.Magnetic post is arranged on the central position of described magnetosheath by this device, and (the two is coaxially arranged, and no matter whether whether magnetic post stretch into the blind end of magnetosheath, magnetic post is in the central position of magnetosheath always), and then provide a magnetic centripetal force to the magnetic bead of magnetosheath blind end, when the centrifugal force produced when centripetal force and magnetosheath rotate reaches a certain trim point, magnetic bead rests on magnetosheath blind end by the effect of centripetal force, and liquid is got rid of de-by centrifugal action, thus reaches the effect of dehydration.
Concrete, this dewatering unit provided by the invention, its agent structure is a housing, housing has one run through and can along the reciprocating main shaft push rod of housing; Under the effect of external force, the magnetic post that main shaft push rod is arranged can extend into the bottom (by the returning device additionally arranged, magnetic post can be made to return further, thus realize the back and forth movement of main shaft push rod and magnetic bead) of magnetosheath; The electric device be arranged in housing then can make magnetosheath rotate motion.
In the process of being carried out applying by this dewatering unit, be separated magnetic bead lysate (containing the magnetic bead adsorbing nucleic acid and lysate) as used it for; Magnetosheath is extend in mixed system, now push main shaft push rod, the end of magnetic bead the is extend into bottom of magnetosheath; Under the effect of magnetic bead, the magnetic bead in mixed system has been adsorbed on magnetosheath, then removes, thus realizes the sharp separation of magnetic bead and mixed system.Further, because magnetosheath is rotary under the drive of electric device, in the process of rotating, achieved the operation (adjustment of centrifugal force can be realized by the rotating speed controlling magnetosheath) of similar centrifuge dehydration like this by the magnetic bead adsorbed, thus make the liquid phase contained by magnetic bead inside separated further.Centrifugal complete after, remove the power putting on main shaft push rod, main shaft push rod is return under the effect of returning device, drives magnetic post to leave from the bottom of magnetosheath; By the magnetic bead that adsorbs owing to losing the adsorption of magnetic force thus departing from from upper magnetosheath, and next step operation can be carried out.
Visible, this dewatering unit provided by the invention, it makes dehydration greatly simplify, substantially reduce the time of dehydration, when skilled operation, dehydration can complete within the several seconds, and the process of whole dehydration can not cause damage to separated material.In addition, this dewatering unit compact construction, is convenient to aseptically operate very much.
Optionally, magnetosheath shack is also comprised; Described magnetosheath shack is arranged on the housing, and its one end and described magnetosheath are socketed; The other end is connected with described electric device.
In the present invention, magnetosheath shack plays the effect be connected with electric device by magnetosheath, thus realizes the effect that electric device drives magnetosheath to rotate more easily.
Optionally, also returning device is comprised; Described returning device is arranged in described housing; And for making described main shaft push rod move to the direction away from described magnetosheath.
The effect of returning device makes main shaft push rod return, namely makes magnetic post leave from the bottom of magnetosheath, and then improve the effect of automated operation.For the concrete structure of returning device, be preferably arranged to an one end and main shaft push rod is fixed, the other end and the fixing spring of housing.By returning device and to the extra applied pressure of main shaft push rod, main shaft push rod (subsidiary magnetic post) back and forth movement can be realized.
Optionally, also circuit card is provided with in described housing; Described electric device is electrically connected with described circuit card, described circuit card is also provided with the motor adjusting knob and charge port that all protrude from described housing; Described housing is also provided with the press button for control circuit board power supply.
The various circuit of circuit card accessible site, by motor adjusting knob, can realize the adjustment of the output rating of electric device, and directly control the slewing rate of magnetosheath shack and magnetosheath; Realize the speed adjustment of magnetosheath; And by charge port (for simplicity, can USB interface be arranged to), realize charging effect can to power supply (being inside located at housing); In addition, housing is also provided with the press button for control circuit board power supply.
Optionally, the one end open of described magnetosheath, the other end is closed, and the cross-sectional area of described magnetosheath is along the direction convergent from opening end to blind end.
In the process of dehydration, magnetosheath can extend in mixture system, and its outer wall can hang with water droplet.Therefore, in order to improve the effect of dehydration, preferably the cross-sectional area of magnetosheath being arranged to the form along the direction convergent from opening end to blind end, rotating in centrifugal process, the magnetosheath with this structure is convenient to adhered to water and is departed from from top to bottom, thus improves dewatering efficiency.
Optionally, the outer wall of one end that described magnetosheath is closed is provided with multiple projection.
Optionally, stating protruding number is even number, and is symmetrical arranged along the direction of described magnetosheath axis.
Can revolve, the distance between two often adjacent described projections is equal.
In addition, in order to increase the grinding effect of magnetosheath in rotation process, preferably, the outside surface of magnetosheath is arranged to asperities.Preferred further, in order to improve the mixing effect of magnetosheath in rotation process, the cecum of magnetosheath can be provided with yi word pattern or cross small-sized fan blade.
The method of described dewatering unit dehydration, comprises the steps:
1), promote main shaft push rod, make described magnetic post arrive the blind end of described magnetosheath, then the magnetosheath of dewatering unit is stretched in the solid-liquid mixture containing magnetic magnetic substance;
2), magnetic substance is adsorbed to the blind end of magnetosheath under the effect of described magnetic post, then removed from solid-liquid mixture by described dewatering unit;
3), control electric device, magnetosheath is rotated, magnetic substance keeps absorbed state by the action of a magnetic field, and magnetic bead surfaces contained humidity is got rid of de-under the influence of centrifugal force.
This dewatering adopts special dewatering unit to complete, for the solid-liquid mixture containing magnetic substance, its can realize between the several seconds magnetic bead and magnetic bead surfaces liquid be separated, and can not damage be caused to separated material, save the time widely, and improve efficiency.
Optionally, in step 1) in, described solid-liquid mixture comprises DNA cleavage liquid containing magnetic bead or elutriant.
For the DNA cleavage liquid comprised containing magnetic bead or elutriant, it is suitable as the handling object of this dewatering unit very much, magnetic bead can all be adsorbed and be removed in a short period of time from mixing embodies, the process of rotating centrifugal also generally can complete between the several seconds, after release magnetic force, magnetic bead can carry out next step operation process, again by Beads enrichment after wash-out, can obtain object nucleic acid, and magnetic bead is deposited after centrifuge dehydration or is again utilized separately.
Utilize above-mentioned device, the present invention can solve an insurmountable difficult problem in this field existing.When utilizing nanometer magnetic bead separating bio material, promote the main shaft push rod of this device, the blind end that described magnetic post can arrive described magnetosheath provides magnetic field, and now magnetosheath extend in the mixing liquid containing magnetic bead as will be described, and magnetic bead can be attracted to outside the blind end of described magnetosheath; Because described magnetic post is positioned at the central position of described magnetosheath, for magnetic bead, described magnetic post provides a magnetic centripetal force.The blind end of magnetosheath proposes liquid level as will be described, and starts rotary knob, and make magnetosheath produce rotary motion, the liquid of rotary motion now to described magnetic bead and its surface will produce centrifugal force.Under the centripetal force effect in magnetic field, keep original position for magnetic bead, liquid then by the impact of magnetic force, do not get rid of de-by the power that is centrifuged, and the interaction of this centrifugal force and centripetal force makes magnetic bead and magnetic bead surfaces liquid serve separating effect.Whole dehydration can complete within the several seconds.
Compared with prior art, beneficial effect of the present invention is:
(1), this device is combined by the centrifugal force produced by the magnetic force of magnetic post with when rotating with magnetosheath, thus realize the disengaging of magnetic bead contained humidity, easier, quick than dewatering types such as general drying, winds, and can not separated material be damaged.
(2), due to this dewatering unit compact construction portable, be therefore suitable as very much the experimental tool of field trial, simultaneously be also convenient to very much aseptically operate.
(3), being further improved (as being arranged to asperities, increase fan blade etc.) to the outside surface of magnetosheath can make this dewatering unit realize the effect of grinding, whipping appts, there is multifunctionality, can be used as a kind of well-adapted experimental tool in field of biology.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 is the structural representation of the dewatering unit in preferred version provided by the invention;
Fig. 2 is the structural representation of the dewatering unit in another technical scheme of the present invention;
Reference numeral:
Housing-101; Main shaft push rod-102; Magnetosheath-103; Electric device-104; Magnetic post-105; Magnetosheath shack-201; Returning device-202; Press button-203; Protruding-204.
Embodiment
Please refer to Fig. 1-Fig. 2, this dewatering unit provided by the invention, it comprises housing 101, main shaft push rod 102, magnetosheath 103 and electric device 104;
Main shaft push rod 102 runs through housing 101, and its two ends all protrude from housing 101; And main shaft push rod 102 can along housing 101 to-and-fro movement; One end of main shaft push rod 102 is provided with magnetic post 105; Magnetosheath 103 enclosure (specifically as shown in Figure 2) on magnetic post 105, and one end of its opening is connected on housing 101; Electric device 104 is arranged in housing 101, and is connected with magnetosheath 103, rotates for making magnetosheath 103.
This dewatering unit provided by the invention, its agent structure is a housing 101, housing 101 has one run through and can along the reciprocating main shaft push rod 102 of housing 101; Under the effect of external force, the magnetic post 105 that main shaft push rod 102 is arranged can extend into the bottom of magnetosheath 103; The electric device 104 be arranged in housing 101 then can make magnetosheath 103 rotate.
When being undertaken in the process applied by this bioseparation device, be separated magnetic bead lysate (containing the magnetic bead adsorbing nucleic acid and lysate) as used it for; Magnetosheath 103 is extend in mixed system, now pushes main shaft push rod 102, make the end of magnetic post 105 extend into the bottom of magnetosheath 103; Under the effect of magnetic post 105, the magnetic post 105 in mixed system has been adsorbed on magnetosheath 103, then removes, thus realizes the sharp separation of magnetic bead and mixed system.Further, because magnetosheath 103 is rotary under the drive of electric device 104, the magnetic bead be attached to outside magnetosheath 103 blind end is retained on magnetosheath 103 under the effect of magnetic force, the liquid of magnetic bead surfaces then under the influence of centrifugal force with Beads enrichment.
Centrifugal complete after, remove the power putting on main shaft push rod 102, main shaft push rod 102 return under the effect of returning device 202, drive magnetic post 105 to leave from the bottom of magnetosheath 103; By the magnetic bead that adsorbs owing to losing the adsorption of magnetic force thus departing from from upper magnetosheath 103, and next step operation can be carried out very expediently.
Visible, this dewatering unit provided by the invention, it makes dehydration greatly simplify, and substantially reduces the time of dehydration, and dehydration can complete within the several seconds, and the process of whole dehydration can not cause damage to separated material.In addition, this dewatering unit compact construction, is convenient to aseptically operate very much.
On the basis of technique scheme, further preferably, magnetosheath shack 201 is also comprised; Magnetosheath shack 201 is arranged on housing 101, and its one end and magnetosheath 103 are socketed; The other end is connected with electric device 104 (also namely magnetosheath 103 realizes being connected with electric device 104 by magnetosheath shack 201, and electric device 104 realizes making magnetosheath 103 rotate motion by Direct driver magnetosheath shack 201).
Magnetosheath shack 201 plays the effect be connected with electric device 104 by magnetosheath 103, thus realizes the effect that electric device 104 drives magnetosheath 103 to rotate more easily.
In biological experiment, for various operating equipment, the height of its level of automation directly affects success or failure and the efficiency of whole experiment; Therefore, in order to improve the automatization performance of this device, on the basis of such scheme, further preferably, returning device 202 is also comprised; Returning device 202 is arranged in housing 101; And for making main shaft push rod 102 move to the direction away from magnetosheath 103; Also circuit card is provided with in housing 101; Electric device 104 is electrically connected with circuit card, circuit card is also provided with the motor adjusting knob and charge port that all protrude from housing 101; Housing 101 is also provided with the press button 203 for control circuit board power supply.
The effect of returning device 202 makes main shaft push rod 102 return, namely makes magnetic post 105 leave from the bottom of magnetosheath 103, and then improve the effect of automated operation.By returning device 202 and the power additionally putting on main shaft push rod 102, main shaft push rod 102 and magnetic post 105 can be realized along housing 101 to-and-fro movement.
For the concrete structure of returning device 202, be preferably arranged to an one end and main shaft push rod 102 is fixed, the spring that the other end is fixed with housing 101.
In addition, it also can be a spring being set on main shaft push rod 102, and one end is fixed by web member and main shaft push rod 102, and the other end and housing 101 are fixed.
Circuit card, motor adjusting knob and press button 203 can control the working order of whole dewatering unit, facilitate user further and use.
In another preferred technical scheme provided by the invention, dewatering unit comprises: housing 101, main shaft push rod 102, magnetosheath 103 and electric device 104, magnetosheath shack 201, returning device 202;
Main shaft push rod 102 runs through housing 101, and its two ends all protrude from housing 101; And main shaft push rod 102 can along housing 101 to-and-fro movement; One end of main shaft push rod 102 is provided with magnetic post 105; Magnetosheath 103 enclosure is on magnetic post 105, and one end of its opening is connected on housing 101; Electric device 104 is arranged in housing 101, and is connected with magnetosheath 103, rotates for making magnetosheath 103;
Magnetosheath shack 201 is arranged on housing 101, and its one end and magnetosheath 103 are socketed; The other end is connected with electric device 104; Returning device 202 is arranged in housing 101; And for making main shaft push rod 102 move to the direction away from magnetosheath 103;
Also circuit card is provided with in housing 101; Electric device 104 is electrically connected with circuit card, circuit card is also provided with the motor adjusting knob and charge port that all protrude from housing 101; Housing 101 is also provided with the press button 203 for control circuit board power supply;
The one end open of magnetosheath 103, the other end is closed, and the cross-sectional area of magnetosheath 103 is along the direction convergent from opening end to blind end; The outer wall of one end that magnetosheath 103 is closed is provided with multiple protruding 204; The number of protruding 204 is even number, and is symmetrical arranged along the direction of magnetosheath 103 axis; Distance between two often adjacent projections 204 is equal.
Have the dewatering unit of said structure, it is multi-functional that it can integrate dehydration, stirring, vortex etc., meets the needs of multiple Bioexperiment.Make this device have grinding function in addition if necessary, then accordingly the outside surface of this magnetosheath 103 is arranged to asperities, if better mixing effect will be realized, then set up yi word pattern or cross small-sized fan blade at the cecum of magnetosheath 103.
Present invention also offers a kind of method utilizing this dewatering unit to dewater, specifically comprise the following steps:
S1, promotion main shaft push rod 102, make described magnetic post 105 arrive the blind end of described magnetosheath 103, then stretch in the solid-liquid mixture containing magnetic magnetic substance by the magnetosheath 103 of dewatering unit;
In this step, solid-liquid mixture comprises DNA cleavage liquid containing magnetic bead or elutriant.
S2, magnetic substance are adsorbed to the blind end of magnetosheath 103 under the effect of described magnetic post 105, are then removed from solid-liquid mixture by described dewatering unit;
In this step, the effect directly removed from mixture system by the magnetic bead adsorbing separated material is achieved; Which, compared with traditional mode removing liquid phase from mixture system, has obvious superiority, the liquid phase after being first separated in magnetic bead remaining less (can naturally vacate under gravity in operating process); In addition, overcome in prior art and liquid phase is absorbed and the defect of the magnetic bead possible loss of existence.
S3, control electric device 104, magnetosheath 103 is rotated, and magnetic substance keeps absorbed state by the action of a magnetic field, and magnetic bead surfaces contained humidity is got rid of de-under the influence of centrifugal force.
The method is based on special dewatering unit, directly separated magnetic bead is removed from mixed solution embodies, thus improve the effect of dehydration, in addition, in the process that magnetosheath 103 rotates, remaining moisture can be thrown out of, further increasing the effect of dehydration, and this dewatering utilizes a device to complete, apparatus and method combine, make operating in the several seconds and can efficiently completing of dehydration, dehydration can not cause any damage to separated material; Therefore there is extraordinary application prospect.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. a dewatering unit, is characterized in that, described dewatering unit comprises: housing, main shaft push rod, magnetosheath and electric device;
Described main shaft push rod runs through described housing, and its two ends all protrude from described housing; And described main shaft push rod can along housing to-and-fro movement; One end of described main shaft push rod is provided with magnetic post; The described magnetosheath enclosure is on described magnetic post, and magnetic post and described magnetosheath are coaxially arranged, and is in the center of described magnetosheath, and one end of described magnetosheath opening connects on the housing;
Described electric device is arranged in described housing, and is connected with described magnetosheath, rotates for making described magnetosheath.
2. dewatering unit according to claim 1, is characterized in that, also comprises magnetosheath shack;
Described magnetosheath shack is arranged on the housing, and its one end and described magnetosheath are socketed; The other end is connected with described electric device.
3. dewatering unit according to claim 1, is characterized in that, also comprises returning device; Described returning device is arranged in described housing; And for making described main shaft push rod move to the direction away from described magnetosheath.
4. dewatering unit according to claim 1 and 2, is characterized in that, is also provided with circuit card in described housing;
Described electric device is electrically connected with described circuit card, described circuit card is also provided with the motor adjusting knob and charge port that all protrude from described housing;
Described housing is also provided with the press button for control circuit board power supply.
5. dewatering unit according to claim 4, is characterized in that, the one end open of described magnetosheath, and the other end is closed, and the cross-sectional area of described magnetosheath is along the direction convergent from opening end to blind end.
6. dewatering unit according to claim 5, is characterized in that, the outer wall of one end that described magnetosheath is closed is provided with multiple projection.
7. dewatering unit according to claim 6, is characterized in that, the number of described projection is even number, and is symmetrical arranged along the direction of described magnetosheath axis.
8. dewatering unit according to claim 7, is characterized in that, the distance between two often adjacent described projections is equal.
9. the method utilizing the dewatering unit described in any one of claim 1-8 to dewater, is characterized in that, comprise the steps:
1), promote main shaft push rod, make described magnetic post arrive the blind end of described magnetosheath, then the magnetosheath of dewatering unit is stretched in the solid-liquid mixture containing magnetic magnetic substance;
2), magnetic substance is adsorbed to the blind end of magnetosheath under the effect of described magnetic post, then removed from solid-liquid mixture by described dewatering unit;
3), control electric device, magnetosheath is rotated, magnetic substance keeps absorbed state by the action of a magnetic field, and magnetic bead surfaces contained humidity is got rid of de-under the influence of centrifugal force.
10. method according to claim 9, is characterized in that, in step 1) in, described solid-liquid mixture comprises DNA cleavage liquid containing magnetic bead or elutriant.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610033982.3A CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
| PCT/CA2016/000204 WO2017139864A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| CA3008660A CA3008660A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| EP16890137.9A EP3405288A4 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| US16/071,038 US20190022667A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610033982.3A CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
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| Publication Number | Publication Date |
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| CN105543090A true CN105543090A (en) | 2016-05-04 |
| CN105543090B CN105543090B (en) | 2018-08-10 |
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| CN201610033982.3A Expired - Fee Related CN105543090B (en) | 2016-01-19 | 2016-01-19 | A kind of dehydration device and method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995000247A1 (en) * | 1993-06-21 | 1995-01-05 | Labsystems Oy | Separation method |
| CN200945454Y (en) * | 2006-06-16 | 2007-09-12 | 复旦大学 | Portable magnetic separating pen |
| CN104344985A (en) * | 2013-08-09 | 2015-02-11 | 英芮诚生化科技（上海）有限公司 | Magnetic separation device capable of sequentially extracting and continuously eluting trace substances |
| CN104646176A (en) * | 2015-02-11 | 2015-05-27 | 英芮诚生化科技（上海）有限公司 | Manual magnetic solid extractor |
| CN105062888A (en) * | 2015-09-02 | 2015-11-18 | 苏州东胜兴业科学仪器有限公司 | Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument |
| CN205473812U (en) * | 2016-01-19 | 2016-08-17 | 山西振东基赛生物科技有限公司 | Dewatering device |
-
2016
- 2016-01-19 CN CN201610033982.3A patent/CN105543090B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995000247A1 (en) * | 1993-06-21 | 1995-01-05 | Labsystems Oy | Separation method |
| CN200945454Y (en) * | 2006-06-16 | 2007-09-12 | 复旦大学 | Portable magnetic separating pen |
| CN104344985A (en) * | 2013-08-09 | 2015-02-11 | 英芮诚生化科技（上海）有限公司 | Magnetic separation device capable of sequentially extracting and continuously eluting trace substances |
| CN104646176A (en) * | 2015-02-11 | 2015-05-27 | 英芮诚生化科技（上海）有限公司 | Manual magnetic solid extractor |
| CN105062888A (en) * | 2015-09-02 | 2015-11-18 | 苏州东胜兴业科学仪器有限公司 | Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument |
| CN205473812U (en) * | 2016-01-19 | 2016-08-17 | 山西振东基赛生物科技有限公司 | Dewatering device |
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