CN105543074B - A kind of fermentation cauldron for microbial fermentation production biosurfactant - Google Patents

A kind of fermentation cauldron for microbial fermentation production biosurfactant Download PDF

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CN105543074B
CN105543074B CN201610015251.6A CN201610015251A CN105543074B CN 105543074 B CN105543074 B CN 105543074B CN 201610015251 A CN201610015251 A CN 201610015251A CN 105543074 B CN105543074 B CN 105543074B
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fermentation
distribution plate
fermentation cauldron
cone
snorkel
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CN105543074A (en
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程叶红
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Jiangxi Zongheng special equipment Co.,Ltd.
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Three Huamai Chemical Products Co Ltd
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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Abstract

The present invention relates to a kind of fermentation cauldron producing biosurfactant for microbial fermentation, the fermentation cauldron for microbial fermentation production biosurfactant；The second agitating shaft is provided in fermentation cauldron, the periphery of the second agitating shaft is uniformly arranged the snorkel of vertical shape arrangement, gas distribution plate is arranged at intervals on the pipe shaft of snorkel.Gas distribution plate is that first cone-shaped hood that Open Side Down is constituted, and opens up through-hole in the middle part of the first cone-shaped hood, is arranged to cavity-like inside the cover body of the first cone-shaped hood, qi-emitting hole is uniformly arranged in the outer cover of the first cone-shaped hood.The fermentation cauldron provided in above-mentioned technical proposal uses it for biological surfactant microemulsion production, the micro emulsion of biosurfactant can quickly be made.

Description

A kind of fermentation cauldron for microbial fermentation production biosurfactant
It is on November 14th, 2014 that the present invention, which is the applying date, application No. is 201410650537.2, entitled " makeup The divisional application of the patent of invention of the biological surfactant microemulsion production system of product, food, chemical industry and agricultural ".
Technical field
The present invention relates to emulsifier production fields, and in particular to a kind of cosmetics, food, chemical industry and agricultural are lived with biological table Agent microemulsion production system.
Background technology
Micro emulsion is the transparent or semitransparent thermodynamic stable system spontaneously formed, there is two kinds of oil-in-water and Water-In-Oil Type.The formation of micro emulsion is spontaneous, does not need extraneous offer energy, is a kind of system that thermodynamics is highly stable, because This is widely used in fields such as petrochemical industry, cosmetics, food.Wherein, the microemulsion prepared by biosurfactant Liquid is usually used in the cosmetics of super quality, more stable, safe, skin absorption is good.The preparation method of such micro emulsion is：It will Biosurfactant (Lipopeptide Biosurfactants), cosurfactant (C3~C5 alcohol) are mixed with alkali (sodium hydroxide) Forming surfactants mixed liquor is prepared, water phase (generally brine) is prepared, prepares oil phase (generally alkane, cycloalkane, fragrance Hydrocarbon)；Prepared surfactant mixed liquor is added in water phase and is uniformly mixed, then oil phase is instilled to above-mentioned solution dropwise In, and constantly stir evenly, until solution by muddiness to clarifying, obtain the micro emulsion being made of biosurfactant, Due to needing using oil phase is added dropwise dropwise, so that the throughput rate bottom of micro emulsion, seriously affects production rate.
Invention content
It is an object of the invention to provide a kind of fermentation cauldrons producing biosurfactant for microbial fermentation.
To achieve the above object, present invention employs following technical schemes：
A kind of fermentation cauldron for microbial fermentation production biosurfactant, which is characterized in that fermentation cauldron, zymotic fluid Purifier apparatus and biosurfactant storage tank are attached successively, and the second rabbling mechanism is provided in fermentation cauldron, fermentation cauldron Top is provided with material input port, and discharge port is arranged in the lower part of fermentation cauldron, and the second rabbling mechanism includes standing the second of shape arrangement to stir Axis is mixed, the periphery of the second agitating shaft is uniformly arranged the snorkel of vertical shape arrangement, air distribution plate is arranged at intervals on the pipe shaft of snorkel Part, offer in the middle part of each gas distribution plate for the second agitating shaft by through-hole, the inside of gas distribution plate be arranged to cavity-like and Uniformly open up qi-emitting hole in the plate face of gas distribution plate, be provided with strainer at each qi-emitting hole, the tube chamber of each snorkel respectively with a cloth The inner cavity of gas plate is connected connection, and the upper end of each snorkel is connected after passing through fermentation cauldron autoclave body with air-blowing device, fermentation cauldron Inside it is additionally provided with temperature sensor and PH measurement assemblies；
Gas distribution plate is that first cone-shaped hood that Open Side Down is constituted, and through-hole, the first cone are opened up in the middle part of the first cone-shaped hood It is arranged to cavity-like inside the cover body of shape cover, qi-emitting hole is uniformly arranged in the outer cover of the first cone-shaped hood.
The biological surfactant microemulsion production system of cosmetics, food, chemical industry and agricultural provided in above-mentioned technical proposal, By carrying out purifying and microemulsified processing to zymotic fluid, to which the micro emulsion of biosurfactant quickly be made.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is the structural schematic diagram of zymotic fluid purifier apparatus；
Fig. 3 is the structural schematic diagram of the second rabbling mechanism, gas distribution plate and snorkel；
Fig. 4 is the structural schematic diagram of the first rabbling mechanism, liquid distribution plate part and oil phase inlet tube；
Fig. 5 is the structural schematic diagram of gas distribution plate；
Fig. 6 is the structural schematic diagram of liquid distribution plate part；
Fig. 7 is the structural schematic diagram of shedding mechanism；
Fig. 8 is the structural schematic diagram of plate face on the downside of the first stripper.
Specific implementation mode
In order to make objects and advantages of the present invention be more clearly understood, the present invention is carried out specifically with reference to embodiments It is bright.It should be appreciated that following word is only used to one or more of specific embodiments of the description present invention, not to the present invention The protection domain specifically asked carries out considered critical.
The technical solution that the present invention takes is as shown in Figure 1, 2, and a kind of cosmetics, food, chemical industry and agricultural are lived with biological table Agent microemulsion production system, which is characterized in that including following production equipment:
The fermentation cauldron 40 of biosurfactant is produced for microbial fermentation；
For carrying out the zymotic fluid purifier apparatus 90 of purifies and separates processing to the zymotic fluid in fermentation cauldron 40 and for storing up Put the biosurfactant storage tank 10 of biosurfactant after purification；
It is respectively used to cosurfactant storage tank 80, the lye storage of storage cosurfactant, lye, water phase and oil phase Tank 50, water phase storage tank 60 and oil phase storage tank 70；
And surfactant Compound mixed solution kettle 20 and micro emulsion reaction kettle 30；Fermentation cauldron, zymotic fluid purifier apparatus It is attached successively with biosurfactant storage tank, biosurfactant storage tank, cosurfactant storage tank and lye The liquid outlet of storage tank is connected with surfactant Compound mixed solution kettle, surfactant Compound mixed solution kettle and water phase storage tank Liquid outlet be connected with micro emulsion reaction kettle.
The biological surfactant microemulsion production system of cosmetics, food, chemical industry and agricultural provided in above-mentioned technical proposal, By carrying out purifying and microemulsified processing to zymotic fluid, to which the micro emulsion of biosurfactant quickly be made.Due to The period of fermentation is longer, and in order to ensure the continuity of production, multiple fermentation cauldrons, the zymotic fluid outlet of multiple fermentation cauldrons can be arranged It is connected respectively with purification devices.
Due to fermentation broth contents complexity, including thalline, the solid medium not being fermented, not by microorganism full utilization Carbohydrate, inorganic salts, protein and the various metabolites of microorganism, fermentation broth viscosity is big, for zymotic fluid purifier apparatus, It specifically can be as shown in Fig. 2, including latter linked dilution device 91, rough filtering device 92, micro-filtration 93, decontamination before setting gradually Albumen device 94, decontamination ion unit 95 and salinization device 96.Dilution device is mainly diluted and adjusts to zymotic fluid PH is generally constituted using groove body；Rough filtering device mainly by zymotic fluid thalline, the solid medium that is not fermented and other Spin-on filter device or other filter plants can be used to getting rid of in larger solid impurity, and multiple filterings can also be used and set Standby to be used in conjunction, the zymotic fluid after thick worry can be diluted and adjust again PH；Micro-filtration mainly removes the small molecular weight impurity in zymotic fluid, Such as pigment is generally carried out using film filter, and the aperture of used filter membrane should be according to purpose biosurfactant Molecular size chosen, should filter membrane retain biosurfactant, ceramic membrane generally can be used；Micro-filtration Later it can be diluted and adjust herein PH；Decontamination albumen device can be isoelectric precipitation device, decontamination ion unit Predominantly resin adsorption device, by being eluted to the MICROBIAL SURFACTANT after absorption, eluent is with lye in salinization Reaction is carried out in device generates salt.It can not certainly for purifier apparatus used by different types of biosurfactant Together, the specific purifier apparatus that this law provides is primarily adapted for use in the purification process of Lipopeptide Biosurfactants.For other The purifying of the biosurfactant (such as carbohydrate, lipoprotein) of type, substantially flow are also dilution, thick worry, micro-filtration, removal Impurity protein and ion.
Since fermentation broth viscosity is big in fermentation cauldron, conventionally employed tracheae is passed through air it is difficult to ensure that filtrated air is being sent out Being uniformly distributed in fermentation tank.Therefore, the present invention is implemented using the technical side as shown in Fig. 3,5：The be arranged in fermentation cauldron The top of two rabbling mechanisms, fermentation cauldron is provided with material input port, and discharge port, the second rabbling mechanism packet is arranged in the lower part of fermentation cauldron The second agitating shaft 41 of vertical shape arrangement is included, the periphery of the second agitating shaft 41 is uniformly arranged the snorkel 45 of vertical shape arrangement, snorkel Be arranged at intervals with gas distribution plate 46 on 45 pipe shaft, the middle part of each gas distribution plate 46 offer for the second agitating shaft 41 by it is logical Hole, the inside of gas distribution plate 46 are arranged to uniformly open up qi-emitting hole 461 in the plate face of cavity-like and gas distribution plate 46, each qi-emitting hole Strainer is provided at 461, the tube chamber of each snorkel is connected with the inner cavity of a gas distribution plate 46 respectively to be connect, each snorkel 45 Upper end is connected after passing through autoclave body with air-blowing device, and temperature sensor and PH measurement assemblies are additionally provided in fermentation cauldron.Stratiform is arranged The gas distribution plate 46 of cloth can supplement air so that air in zymotic fluid when blasting filtrated air from each layer phase zymotic fluid Uniformly, ensure the fermentation of biosurfactant.
Preferably scheme is：A cone-shaped hood that Open Side Down may be used and constitute gas distribution plate, opened up in the middle part of cone-shaped hood Through-hole is arranged to cavity-like inside the cover body of cone-shaped hood, qi-emitting hole 461 is uniformly arranged in the outer cover of cone-shaped hood.Such gas distribution Uniformity and horizontal covering surface it is maximum, as shown in Figure 5.There are three the gas distribution plate stratiform settings, snorkel setting There are three, the upper end of snorkel passes through four-pass connecting joint and tracheae and drum after the motor mount on fermentation cauldron autoclave body top Device of air is connected.
It is further as shown in Figure 7,8, shedding mechanism is provided at discharge port, shedding mechanism includes at fermentation tank discharge port Circular first and second stripper 42,43 being arranged in parallel above and below, the first stripper 42 are fixed with one with fermentation tank, and second unloads Offer annular groove on the side of flitch 43, the second stripper 43 by the steel ball component 48 that is arranged in annular groove and fermentation tank around Vertical direction constitutes the connection that is rotatably assorted, and the center of circle of the first and second stripper 42,43 is located on same plumb line, the first and second discharging Open up first and second discharge port 42a, 43a, the lower face setting of the first stripper 42 on plate at the circumference of same radius size respectively Step-like, the upper face in second step face 422 and the second stripper 43 is constituted at by the combination of the first and second step surface 421,422 It fits, forms cavity between 43 upper face of First terrace 421 and the second stripper, the first discharge port 42a is opened in first On step surface 421, sealing element 423, the shape of sealing element 423 and first and second are provided at the step that the first and second step surface is joined The step shape of step surface junction is consistent, and the second stripper 43 is connected with driving mechanism, and preferred scheme is：First and second The outer profile of step surface junction is arc, sets sealing element to arc in this way, and sealing element can be used O-ring seals, first The height that the upper face of stripper is arranged at taper and the first discharge port is minimum.By driving mechanism adjust the second stripper into Row rotation so that the first and second discharge port on the first and second stripper is corresponding and different, to realize to the quick of fermentation tank The closure of discharging and discharge port realizes quickly discharging material, to save the time, improves extraction efficiency.
In order to improve the rate of microemulsified, the present invention is implemented using the technical side as shown in Fig. 4,6：Micro emulsion is anti- It includes the first stirring of vertical shape arrangement to answer the first rabbling mechanism being arranged in kettle, including autoclave body and autoclave body, the first rabbling mechanism Axis 31, the periphery of the first agitating shaft 31 are uniformly arranged the oil phase inlet tube 35 of vertical shape arrangement, on the pipe shaft of oil phase inlet tube 35 between Every being provided with liquid distribution plate part 36, the middle part of each liquid distribution plate part 36 offer for the first agitating shaft 31 by through-hole, liquid distribution plate part 36 inside is arranged to cavity-like and the lower face of liquid distribution plate part 36 uniformly opens up liquid distributing hole, the tube chamber difference of each oil phase inlet tube It is connected with the inner cavity of a liquid distribution plate part 36 and connect, the upper end of each oil phase inlet tube is connected after autoclave body with oil phase storage tank, Surfactant mixed liquor feed liquor nozzle and water phase feed liquor nozzle are additionally provided on autoclave body.The liquid distribution plate part 36 of stratiform arrangement, When oil phase is added dropwise, oil phase is instilled from each liquid distributing hole on liquid distribution plate part 36 in the mixed liquor in reaction kettle, relative to traditional One after another drop of instillation significantly improves production efficiency.Certain spacing should be kept between each liquid distributing hole, when specific operation, liquid distributing hole is The needle tubing plugged on liquid distribution plate part 36 is constituted, and the length of adjacent needle tubing is different.
Preferably scheme is：Liquid distribution plate part 36 is that the cone-shaped hood of an opening upwards is constituted, and through-hole is opened up in the middle part of cone-shaped hood, It is arranged to cavity-like inside the cover body of cone-shaped hood, the needle tubing 361 for constituting qi-emitting hole is uniformly arranged in the outer cover of cone-shaped hood, The length direction of needle tubing 361 and the perpendicular arrangement of the cover of cone-shaped hood, needle tubing is arranged on the cover of cone-shaped hood in round, and needle The length of pipe increases with the radius of cone-shaped hood and is increased, and instills the best results of oil phase in this way.The liquid distribution plate part stratiform is set There are three setting, oil phase inlet tube is provided with three, and the upper end of oil phase inlet tube passes through four-pass connecting joint and material after autoclave body Liquid conveyance conduit is connected with oil phase storage tank.
Due to being respectively provided with distribution device and liquid distributor, the first and second agitating shaft axle body on the first and second agitating shaft On agitating paddle can not be set, therefore the first and second rabbling mechanism is implemented using identical technical solution in the present invention, Yi Jiru Fig. 3, shown in 4, the radial setting connecting rod in lower end of the first and second agitating shaft 31,41, the end of connecting rod is stood shape setting and is stirred respectively Bar is mixed, the agitating paddle 34 of stirring feed liquid is respectively set in connecting rod and/or stirring rod.The length for being specifically stirring rod is connection Twice of bar, connecting rod include the first, second and third connecting rod 324,322,323, stirring rod include the first, second and third stirring rod 334, 332,333, stirring is respectively set in the epimere of head rod 324, the hypomere of the second stirring rod 332 and third stirring rod 333 Paddle 34, agitating paddle 34 are that a plate is constituted, and such rabbling mechanism can from different directions be stirred solution, improve mixed The uniformity of conjunction.Stirring rod and connecting rod are that angle steel is constituted, and such intensity is big, the installation convenient for agitating paddle 34 and blender The stability of structure.
In addition, for fermentation cauldron, the kettle wall of fermentation cauldron is sandwich, and the clip cavity that sandwich is formed is for accommodating hot-fluid Body.Hot fluid meets the needs of microbial fermentation for adjusting fermentation temperature in the kettle.For micro emulsion reaction kettle, autoclave body Flow-interfering bar is provided on madial wall, the side wall of autoclave body is constituted for sandwich, and the clip cavity that sandwich is formed is for accommodating hot-fluid Body.Hot fluid adapts to the production of different micro emulsions for adjusting temperature in the kettle.
Third rabbling mechanism and ultrasound emission component, surfactant are provided in surfactant Compound mixed solution kettle The kettle wall for preparing kettle is sandwich, the clip cavity of sandwich formation for accommodating heating fluid.Ultrasound emission component is to material It is vibrated so that the quick mixing of surfactant, cosurfactant.
In short, the present invention can effectively improve the production efficiency of micro emulsion.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, after knowing content described in the present invention, without departing from the principle of the present invention, it can also be made several Same transformation and replacement, these convert and substitute on an equal basis and also should be regarded as belonging to the scope of protection of the present invention.

Claims (2)

1. a kind of fermentation cauldron for microbial fermentation production biosurfactant, which is characterized in that fermentation cauldron, zymotic fluid are pure Change equipment and biosurfactant storage tank to be attached successively, be provided with the second rabbling mechanism in fermentation cauldron, fermentation cauldron it is upper Portion is provided with material input port, and discharge port is arranged in the lower part of fermentation cauldron, and the second rabbling mechanism includes the second stirring of vertical shape arrangement Axis, the periphery of the second agitating shaft are uniformly arranged the snorkel of vertical shape arrangement, gas distribution plate are arranged at intervals on the pipe shaft of snorkel, Offered in the middle part of each gas distribution plate for the second agitating shaft by through-hole, the inside of gas distribution plate is arranged to cavity-like and gas distribution Uniformly open up qi-emitting hole in the plate face of plate, be provided with strainer at each qi-emitting hole, the tube chamber of each snorkel respectively with an air distribution plate The inner cavity of part is connected connection, and the upper end of each snorkel is connected after passing through fermentation cauldron autoclave body with air-blowing device, in fermentation cauldron also It is provided with temperature sensor and PH measurement assemblies；
Gas distribution plate is that first cone-shaped hood that Open Side Down is constituted, and through-hole, the first cone-shaped hood are opened up in the middle part of the first cone-shaped hood Cover body inside be arranged to cavity-like, be uniformly arranged qi-emitting hole in the outer cover of the first cone-shaped hood.
2. the fermentation cauldron according to claim 1 for microbial fermentation production biosurfactant, it is characterised in that： There are three the gas distribution plate stratiform settings, and snorkel is provided with three, and the upper end of snorkel passes through fermentation cauldron autoclave body top Motor mount after be connected with air-blowing device by four-pass connecting joint and tracheae.
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