CN105482997A - Fermentation kettle for biological surface active agent microemulsion production - Google Patents

Fermentation kettle for biological surface active agent microemulsion production Download PDF

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Publication number
CN105482997A
CN105482997A CN201610017017.7A CN201610017017A CN105482997A CN 105482997 A CN105482997 A CN 105482997A CN 201610017017 A CN201610017017 A CN 201610017017A CN 105482997 A CN105482997 A CN 105482997A
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distribution plate
fermentation cauldron
fermentation
ventpipe
gas distribution
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CN105482997B (en
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程叶红
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Guangdong Forestry Industrial Equipment Co ltd
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/18—Stationary reactors having moving elements inside
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/82—Combinations of dissimilar mixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/82—Combinations of dissimilar mixers
    • B01F33/821—Combinations of dissimilar mixers with consecutive receptacles
    • B01F33/8212—Combinations of dissimilar mixers with consecutive receptacles with moving and non-moving stirring devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0053—Details of the reactor
    • B01J19/0066—Stirrers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J4/00—Feed or outlet devices; Feed or outlet control devices
    • B01J4/001—Feed or outlet devices as such, e.g. feeding tubes
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    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K23/00—Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
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    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K23/00—Use of substances as emulsifying, wetting, dispersing, or foam-producing agents
    • C09K23/017—Mixtures of compounds
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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    • C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
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Abstract

The invention relates to a fermentation kettle for biological surface active agent microemulsion production. The fermentation kettle is used for microbial fermentation. A second stirring mechanism is arranged inside the fermentation kettle and comprises a vertically-arranged second stirring shaft. Vertically-arranged breather pipes are evenly arranged on the periphery of the second stirring shaft. Air distribution plate pieces are arranged on bodies of the breather pipes at intervals. A through hole through which the second stirring shaft passes is formed in the middle of each air distribution plate piece. The air distribution plate pieces are hollow, and air distribution holes are evenly formed in the faces of the air distribution plate pieces. A filter net is arranged at the position of each air distribution hole. A pipe cavity of each breather pipe is communicated and connected with an inner cavity of one air distribution plate piece. The upper end of each breather pipe penetrates through a body of the fermentation tank to be connected with an air blowing device. According to the technical scheme, the fermentation kettle is used for biological surface active agent microemulsion production, and therefore microemulsion of a biological surfactant is rapidly obtained.

Description

A kind of biological surfactant microemulsion production fermentation cauldron
The present invention is the applying date is on November 14th, 2014, and application number is 201410650537.2, and denomination of invention is the divisional application of the patent of invention of " the biological surfactant microemulsion production system of makeup, food, chemical industry and agricultural ".
Technical field
The present invention relates to emulsifying agent production field, be specifically related to a kind of biological surfactant microemulsion production fermentation cauldron.
Background technology
Microemulsion is the thermodynamic stable system of transparent or semitransparent spontaneous formation, has oil-in-water and water-in-oil two type.The formation of microemulsion is spontaneous, does not need the external world to provide energy, is the highly stable system of a kind of thermodynamics, is therefore adopted widely in fields such as petrochemical complex, makeup, food.Wherein, the microemulsion prepared by bio-surfactant is usually used in the cosmetics of super quality, and it is more stable, security is high, skin absorption is good.The preparation method of such microemulsion is: by bio-surfactant (Lipopeptide Biosurfactants), cosurfactant (C3 ~ C5 alcohol) and alkali (sodium hydroxide) mixed preparing forming surfactants mixed solution, preparation aqueous phase (being generally salt solution), preparation oil phase (being generally alkane, naphthenic hydrocarbon, aromatic hydrocarbon); The tensio-active agent mixed solution prepared is added in aqueous phase and mixes, then oil phase is dropwise instilled in above-mentioned solution, and constantly stir, until solution by muddiness to clarification, the i.e. obtained microemulsion be made up of bio-surfactant, dropwise drip oil phase because needs adopt, therefore make, at the bottom of the throughput rate of microemulsion, to have a strong impact on products production speed.
Summary of the invention
Object of the present invention is just to provide a kind of biological surfactant microemulsion production fermentation cauldron.
For achieving the above object, present invention employs following technical scheme:
A kind of biological surfactant microemulsion production fermentation cauldron, it is characterized in that, fermentation cauldron, fermented liquid purifier apparatus is connected successively with bio-surfactant storage tank, the second stirring mechanism is provided with in fermentation cauldron, the top of fermentation cauldron is provided with material input port, the bottom of fermentation cauldron arranges discharge opening, second stirring mechanism comprises the second stir shaft that vertical shape is arranged, the periphery of the second stir shaft evenly arranges the ventpipe of vertical shape layout, the pipe shaft of ventpipe is arranged at intervals with gas distribution plate, the middle part of each gas distribution plate offers the through hole passed through for the second stir shaft, the inside of gas distribution plate is arranged to cavity-like and qi-emitting hole is evenly offered in the plate face of gas distribution plate, each qi-emitting hole place is provided with filter screen, the tube chamber of each ventpipe is connected with the intracavity inter-connection of a gas distribution plate respectively, the upper end of each ventpipe is connected with air-blowing device through after fermentation cauldron kettle, temperature sensor and PH measurement components is also provided with in fermentation cauldron.
The biological surfactant microemulsion production system of the makeup provided in technique scheme, food, chemical industry and agricultural, by carrying out purifying and microemulsified process to fermented liquid, thus obtains the microemulsion of bio-surfactant fast.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the structural representation of fermented liquid purifier apparatus;
Fig. 3 is the structural representation of the second stirring mechanism, gas distribution plate and ventpipe;
Fig. 4 is the structural representation of the first stirring mechanism, liquid distribution plate part and oil phase liquid-inlet pipe;
Fig. 5 is the structural representation of gas distribution plate;
Fig. 6 is the structural representation of liquid distribution plate part;
Fig. 7 is the structural representation of shedding mechanism;
Fig. 8 is the structural representation in the first stripper lower side panel face.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is specifically described.Should be appreciated that following word only in order to describe one or more concrete embodiments of the present invention, considered critical is not carried out to the protection domain that the present invention specifically asks.
As shown in Figure 1, 2, the biological surfactant microemulsion production system of a kind of makeup, food, chemical industry and agricultural, is characterized in that, comprise following production unit the technical scheme that the present invention takes:
The fermentation cauldron 40 of bio-surfactant is produced for fermentable;
For carrying out the fermented liquid purifier apparatus 90 of purifies and separates process and the bio-surfactant storage tank 10 for storing the bio-surfactant after purifying to the fermented liquid in fermentation cauldron 40;
Be respectively used to store the cosurfactant storage tank 80 of cosurfactant, alkali lye, aqueous phase and oil phase, alkali liquor storage tank 50, aqueous phase storage tank 60 and oil phase storage tank 70;
And tensio-active agent Compound mixed solution still 20 and microemulsion reactor 30; Fermentation cauldron, fermented liquid purifier apparatus are connected successively with bio-surfactant storage tank, the liquid outlet of bio-surfactant storage tank, cosurfactant storage tank and alkali liquor storage tank is all connected with tensio-active agent Compound mixed solution still, and tensio-active agent Compound mixed solution still is all connected with microemulsion reactor with the liquid outlet of aqueous phase storage tank.
The biological surfactant microemulsion production system of the makeup provided in technique scheme, food, chemical industry and agricultural, by carrying out purifying and microemulsified process to fermented liquid, thus obtains the microemulsion of bio-surfactant fast.Because the cycle of fermentation is longer, in order to ensure the continuity of producing, can arrange multiple fermentation cauldron, the fermented liquid outlet of multiple fermentation cauldron is connected with purification devices respectively.
Because fermentation broth contents is complicated, comprise thalline, the solid medium be not fermented, not by carbohydrate, inorganic salt, protein that microorganism utilizes completely, and the various meta-bolitess of microorganism, fermentation broth viscosity is large, for fermented liquid purifier apparatus, specifically as shown in Figure 2, can comprise and set gradually front latter linked air mix facilities 91, rough filtering device 92, micro-filtration 93, decon albumen device 94, decon ion unit 95 and salt gasifying device 96.Air mix facilities mainly dilutes fermented liquid and regulates PH, and the general cell body that adopts is formed; Rough filtering device mainly by the thalline in fermented liquid, the solid medium be not fermented and other larger solid impurities to getting rid of, spin-on filter device or other filter plants can be adopted, also multiple filter plant can be adopted to be used in conjunction, and the fermented liquid after thick worry can carry out diluting and regulating PH again; Micro-filtration mainly removes the small molecular weight impurity in fermented liquid, as pigment etc., general employing membrane filter appts carries out, adopt the aperture of filtering membrane should choose according to the molecular size of object bio-surfactant, filtering membrane should be made to retain bio-surfactant, generally can adopt ceramic membrane; Can carry out diluting and regulating PH at this after micro-filtration; Decon albumen device can be isoelectric precipitation device, and decon ion unit is mainly resin absorption device, and by carrying out wash-out to the MICROBIAL SURFACTANT after absorption, elutriant and alkali lye carry out reaction and generates salt in salt gasifying device.Certainly the purifier apparatus adopted for dissimilar bio-surfactant can be different, and the concrete purifier apparatus that this law provides mainly is applicable to the purification process of Lipopeptide Biosurfactants.For the purifying of the bio-surfactant (as carbohydrate, lipoprotein) of other kinds, its cardinal principle flow process is also dilution, thick worry, micro-filtration, removal impurity protein and ion.
Because fermentation broth viscosity in fermentation cauldron is large, therefore tradition adopts tracheae to pass into air to be difficult to ensure sterile air being uniformly distributed in fermentor tank.Therefore, the present invention adopts as Fig. 3, technical side shown in 5 implements: the second stirring mechanism arranged in fermentation cauldron, the top of fermentation cauldron is provided with material input port, the bottom of fermentation cauldron arranges discharge opening, second stirring mechanism comprises the second stir shaft 41 that vertical shape is arranged, the periphery of the second stir shaft 41 evenly arranges the ventpipe 45 of vertical shape layout, the pipe shaft of ventpipe 45 is arranged at intervals with gas distribution plate 46, the middle part of each gas distribution plate 46 offers the through hole passed through for the second stir shaft 41, the inside of gas distribution plate 46 is arranged to cavity-like and qi-emitting hole 461 is evenly offered in the plate face of gas distribution plate 46, each qi-emitting hole 461 place is provided with filter screen, the tube chamber of each ventpipe is connected with the intracavity inter-connection of a gas distribution plate 46 respectively, the upper end of each ventpipe 45 is connected with air-blowing device through after kettle, temperature sensor and PH measurement components is also provided with in fermentation cauldron.The gas distribution plate 46 of arrange themselves, when blasting sterile air, can supplement air, making even air in fermented liquid from each layer phase fermented liquid, ensures the fermentation of bio-surfactant.
Preferred scheme is: a cone-shaped hood that Open Side Down can be adopted to form gas distribution plate, and through hole is offered at the middle part of cone-shaped hood, and cavity-like is arranged in the cover body inside of cone-shaped hood, and the outer cover of cone-shaped hood evenly arranges qi-emitting hole 461.The homogeneity of such gas distribution, and horizontal coverage rate is maximum, as shown in Figure 5.Described gas distribution plate stratiform is provided with three, and ventpipe is provided with three, and the upper end of ventpipe is connected with air-blowing device by four-pass connecting joint and tracheae through after the motor mount on fermentation cauldron kettle top.
Further as Fig. 7, shown in 8, discharge opening place is provided with shedding mechanism, and shedding mechanism comprises in fermentor tank discharge opening place, under be arranged in parallel first of circle, two strippers 42, 43, first stripper 42 and fermentor tank are fixed with one, and the side of the second stripper 43 offers ring groove, and the second stripper 43 consists of to be rotatably assorted around vertical the steel ball assembly 48 that arranges in ring groove and fermentor tank and is connected, and first, two strippers 42, the center of circle of 43 is positioned on same plummet, and first, on two strippers, the circumference place of same radius size offers first respectively, two discharge opening 42a, 43a, the lower face of the first stripper 42 is arranged to by first, two step surfaces 421, it is step-like that 422 combinations are formed, and the upper face of second step face 422 and the second stripper 43 fits, and form cavity between First terrace 421 and the second stripper 43 upper face, the first discharge opening 42a is opened on First terrace 421, and first, the step place that two step surfaces are joined is provided with sealing member 423, the shape of sealing member 423 and first, the step shape of two step surface junctions is consistent, and the second stripper 43 is connected with driving mechanism, and preferred scheme is: first, the outline of two step surface junctions is arc, like this sealing member is set to arc, and sealing member can adopt O-ring seals, and the upper face of the first stripper is arranged to taper and the height at the first discharge opening place is minimum.The second stripper is regulated to rotate by driving mechanism, make first and second discharge opening on first and second stripper corresponding and different, thus realize, to the quickly discharging material of fermentor tank and the closed of discharge opening, realizing quickly discharging material, thus save time, improve extraction efficiency.
In order to improve the speed of microemulsified, the present invention adopts as Fig. 4, technical side shown in 6 implements: microemulsion reactor, comprise the first stirring mechanism arranged in kettle and kettle, first stirring mechanism comprises the first stir shaft 31 that vertical shape is arranged, the periphery of the first stir shaft 31 evenly arranges the oil phase liquid-inlet pipe 35 of vertical shape layout, the pipe shaft of oil phase liquid-inlet pipe 35 is arranged at intervals with liquid distribution plate part 36, the middle part of each liquid distribution plate part 36 offers the through hole passed through for the first stir shaft 31, the inside of liquid distribution plate part 36 is arranged to cavity-like and the lower face of liquid distribution plate part 36 evenly offers liquid distributing hole, the tube chamber of each oil phase liquid-inlet pipe is connected with the intracavity inter-connection of a liquid distribution plate part 36 respectively, the upper end of each oil phase liquid-inlet pipe is connected with oil phase storage tank through after kettle, kettle is also provided with the tensio-active agent mixed solution feed liquor mouth of pipe and the aqueous phase feed liquor mouth of pipe.The liquid distribution plate part 36 of arrange themselves, when dripping oil phase, oil phase, from the mixed solution in each liquid distributing hole instillation reactor liquid distribution plate part 36, significantly improves production efficiency relative to traditional one after another drop of instillation.Should keep certain spacing between each liquid distributing hole, during concrete operations, liquid distributing hole is that the needle tubing that liquid distribution plate part 36 plugs is formed, and the length of adjacent needle tubing is different.
Preferred scheme is: liquid distribution plate part 36 is that the cone-shaped hood of an opening upwards is formed, through hole is offered at the middle part of cone-shaped hood, cavity-like is arranged in the cover body inside of cone-shaped hood, the outer cover of cone-shaped hood is evenly arranged the needle tubing 361 for forming qi-emitting hole, the length direction of needle tubing 361 and the perpendicular layout of the cover of cone-shaped hood, needle tubing is arranged in round on the cover of cone-shaped hood, and the length of needle tubing increases with the radius increase of cone-shaped hood, instills the best results of oil phase like this.Described liquid distribution plate part stratiform is provided with three, and oil phase liquid-inlet pipe is provided with three, and the upper end of oil phase liquid-inlet pipe is connected with oil phase storage tank by four-pass connecting joint and feed liquid transport pipe through after kettle.
Due to first and second stir shaft being provided with distribution device and liquid distributor respectively, therefore first and second stir shaft axle body cannot arrange stirring rake, therefore in the present invention, first and second stirring mechanism adopts identical technical scheme to implement, that is as shown in Figure 3,4, the lower end of first and second stir shaft 31,41 is radial arranges union lever, the end of union lever founds shape respectively and arranges agitator arm, and union lever and/or agitator arm arrange the stirring rake 34 stirring feed liquid respectively.The concrete length for agitator arm is the twice of union lever, union lever comprises first, second and third union lever 324,322,323, agitator arm comprises first, second and third agitator arm 334,332,333, the hypomere of head rod 324, second agitator arm 332 and the epimere of the 3rd agitator arm 333 arrange stirring rake 34 respectively, stirring rake 34 is that a plate is formed, such stirring mechanism can stir solution from different directions, improves the homogeneity of mixing.Agitator arm and union lever are angle steel and form, and such intensity is large, is convenient to the installation of stirring rake 34 and the stability of stirring mechanism.
In addition, for fermentation cauldron, the still wall of fermentation cauldron is sandwich structure, and the folder chamber that sandwich structure is formed is for holding hot-fluid.Hot-fluid, for regulating fermentation temperature in the kettle, meets the demand of fermentable.For microemulsion reactor, the inner side-wall of kettle is provided with flow-interfering bar, the sidewall of kettle is that sandwich structure is formed, and the folder chamber that sandwich structure is formed is for holding hot-fluid.Hot-fluid, for regulating temperature in the kettle, adapts to the production of different microemulsion.
Be provided with the 3rd stirring mechanism and ultrasound emission assembly in tensio-active agent Compound mixed solution still, the still wall of surfactant formulatory still is sandwich structure, folder chamber that sandwich structure is formed is for holding heating fluid.Ultrasound emission assembly vibrates to material, makes tensio-active agent, cosurfactant mixes fast.
In a word, the present invention effectively can improve the production efficiency of microemulsion.
The above is only the preferred embodiment of the present invention; should be understood that; for those skilled in the art; to know in the present invention after contents; under the premise without departing from the principles of the invention; can also make some equal conversion to it and substitute, these convert on an equal basis and substitute and also should be considered as belonging to protection scope of the present invention.

Claims (2)

1. a biological surfactant microemulsion production fermentation cauldron, it is characterized in that, fermentation cauldron, fermented liquid purifier apparatus is connected successively with bio-surfactant storage tank, the second stirring mechanism is provided with in fermentation cauldron, the top of fermentation cauldron is provided with material input port, the bottom of fermentation cauldron arranges discharge opening, second stirring mechanism comprises the second stir shaft that vertical shape is arranged, the periphery of the second stir shaft evenly arranges the ventpipe of vertical shape layout, the pipe shaft of ventpipe is arranged at intervals with gas distribution plate, the middle part of each gas distribution plate offers the through hole passed through for the second stir shaft, the inside of gas distribution plate is arranged to cavity-like and qi-emitting hole is evenly offered in the plate face of gas distribution plate, each qi-emitting hole place is provided with filter screen, the tube chamber of each ventpipe is connected with the intracavity inter-connection of a gas distribution plate respectively, the upper end of each ventpipe is connected with air-blowing device through after fermentation cauldron kettle, temperature sensor and PH measurement components is also provided with in fermentation cauldron.
2. biological surfactant microemulsion production fermentation cauldron according to claim 1, it is characterized in that, described gas distribution plate stratiform is provided with three, ventpipe is provided with three, and the upper end of ventpipe is connected with air-blowing device by four-pass connecting joint and tracheae through after the motor mount on fermentation cauldron kettle top.
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