CN105532394B - Utilize the method for seedling stage white root screening rice heat resistant variety - Google Patents
Utilize the method for seedling stage white root screening rice heat resistant variety Download PDFInfo
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- CN105532394B CN105532394B CN201510908336.2A CN201510908336A CN105532394B CN 105532394 B CN105532394 B CN 105532394B CN 201510908336 A CN201510908336 A CN 201510908336A CN 105532394 B CN105532394 B CN 105532394B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of methods using seedling stage white root screening rice heat resistant variety.When different rice varieties are carried out water planting to the 4 leaf phase by this method, high-temperature process is carried out, after treatment detects the difference of different cultivars root system white root, calculates heat-resistance coefficient, the heat resistance difference of different cultivars is determined, to quickly screen heat resistant variety.The present invention utilizes Seedling root index screening rice heat resistant variety, can screen in early days to rice varieties heat resistance indoors, save a large amount of man power and material, Objective is more obvious.
Description
Technical field
The present invention relates to a kind of methods using seedling stage white root screening rice heat resistant variety.
Background technique
As the trend of global warming is increasingly severe, high temperature also increasingly obtains the weight of people to the harm of crop
Depending on up to the present, relatively fuzzyyer to the understanding of the Climatic regionalization mechanism of action, this is also that reply heat evil increases difficulty in production
Degree.China In Middle And Lower Reaches of Changjiang River is the severely afflicated area of rice high temperature damage, and in recent years, high temperature damage is more and more serious, especially
With the increasingly increase of the popularizing area of hybrid paddy rice, heat evil has threatened the production in this important goods grain base of the Yangtze river basin
Safety.Practice have shown that breeding high temperature resistant kind is to solve the effective means of rice Climatic regionalization.
Rice heat resistant variety screening at present has been achieved for certain progress, has filtered out a collection of heat resistant variety for breeding
Practice, but the method for heat resistant variety screening at present still remains defect, ignores the under ground portion i.e. choosing of root system of rice
Select effect.We have found that seedling stage has differences rice high-temperature process, different its root system of heat resistant variety in research carries out,
With this, we have proposed a kind of methods for screening rice heat resistant variety using white root for screening index.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of methods using seedling stage white root screening rice heat resistant variety.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is that, it is resistance to that rice is screened using seedling stage white root
The method of hot kind, comprising the following steps:
(1) seed of the sprouting after more seed soaking disinfections is homogeneously disposed in even number culture dish by same rice varieties
Water planting is carried out, and using the half of the even number culture dish of the rice varieties as high-temperature process group, the other half culture dish conduct
Room temperature control group；
(2) until in culture dish when water-culturing rice to the 4 leaf phase, by high-temperature process group be placed in incubator by the following conditions into
Row high-temperature process 1~4 day:
When 8 whens of the morning~afternoon 6,45 DEG C；
When 6 whens of afternoon~the next morning 8,32 DEG C；
The cultivating seedling of room temperature control group is placed in the incubator of another same model simultaneously, and and high-temperature process
The identical intensity of illumination of group, light application time, humidity and CO2Concentration, temperature are controlled at 32 DEG C, and lasting culture is identical as high-temperature process
Number of days；
(3) after treatment takes several strains of cultivating seedling by same rice varieties in each culture dish of high-temperature process group,
Room temperature control group also chooses the cultivating seedling of identical strain number in the same way；
(4) it will be sequentially placed into root system scanning system and be scanned behind the clean root of the cultivating seedling of acquirement, distinguish by kind
Count the white root of room temperature control group and the white root of high-temperature process group；
(5) heat-resistance coefficient of different cultivars is calculated as follows:
Heat-resistance coefficient=room temperature control group white root-high-temperature process group white root)/room temperature control group white root ×
100%
Kind heat resistance difference is determined from high to low with heat-resistance coefficient, and the higher kind of heat-resistance coefficient, heat resistance is better.
Preferably, by the seed of the sprouting after seed soaking disinfection, uniform pendulum is covered in diameter 20cm in step (1)
In the culture dish of blotting paper, the grain number sowed in each culture dish is 40, carries out water planting, and each rice varieties carry out water planting
Culture dish is 12, wherein 6 are high-temperature process group, remaining 6 are room temperature control group.
Preferably, in step (3), after treatment, in each culture dish of high-temperature process group of each rice varieties
Intermediate 10 plants of cultivating seedling are taken, 6 culture dishes take 60 plants of cultivating seedling altogether, and room temperature control group also chooses 60 plants of trainings in the same way
Support seedling.
The beneficial effects of the present invention are:
1, using Seedling root index screening rice heat resistant variety, rice varieties heat resistance can be carried out in early days indoors
Screening, saves a large amount of man power and material, Objective is more obvious.
2, it uses white root to carry out heat resistant variety screening for index by way of water planting, of tested variety can be increased
Number shortens the period of rice heat resistance screening, so as to greatly speed up the speed and accuracy of heat resistant variety screening.
3, heat resistant variety is screened using indoor seedling stage white root, avoided with setting percentage screening heat resistant variety by different year
Weather conditions it is inconsistent and caused by the poor problem of experimental result repeatability, further ensure the reliable of heat resistant variety screening
Property.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is that 2 kinds of different cultivars of the embodiment of the method that the present invention utilizes seedling stage white root screening rice heat resistant variety are resistance to
The situation of change of hot coefficient.
Marked in the figure:
1) 1-17 respectively represents following 17 rice varieties: BALILLA, DULAR, CR547-1-2-3, CUIABANA,
GIZA178、GZ948-2-2-1、IDSA77、IR2307-247-2-2-3、IR1561-228-3-3、IR36、IR50、IR60、
KCN80152、N22、SAKHA104、TCHAMPA、WAB56-125、WAB96-1-1。
2) series 1 is the heat-resistance coefficient based on white root, and series 2 is the heat-resistance coefficient based on setting percentage.
Specific embodiment
17 different heat resistant varieties that selection has been filtered out using setting percentage as heat-resistance coefficient: BALILLA,
DULAR、CR547-1-2-3、CUIABANA、GIZA178、GZ948-2-2-1、IDSA77、IR2307-247-2-2-3、
IR1561-228-3-3、IR36、IR50、IR60、KCN80152、N22、SAKHA104、TCHAMPA、WAB56-125、WAB96-
1-1, seedling stage water planting after 4 leaf phase high-temperature process, detect white root, calculate heat-resistance coefficient as follows:
Heat-resistance coefficient=(white root of room temperature control group-high-temperature process group white root)/room temperature control group white root
× 100%
Using the above-mentioned heat-resistance coefficient being calculated as Testing index, the heat resistance of different heat resistant varieties is analyzed, the results showed that,
It is almost the same using the heat-resistance coefficient based on white root and the heat-resistance coefficient variation tendency based on setting percentage, it can screen
The heat resistance difference (Fig. 1) of rice varieties.
The embodiments of the present invention described above are not intended to limit the scope of the present invention.It is any in the present invention
Spirit and principle within made modifications, equivalent substitutions and improvements etc., should be included in claim protection model of the invention
Within enclosing.
Claims (3)
1. utilizing the method for seedling stage white root screening rice heat resistant variety, comprising the following steps:
(1) seed of the sprouting after more seed soaking disinfections is homogeneously disposed in even number culture dish and is carried out by same rice varieties
Water planting, and using the half of the even number culture dish of the rice varieties as high-temperature process group, the other half culture dish is as room temperature
Control group；
(2) until in culture dish when water-culturing rice to the 4 leaf phase, high-temperature process group is placed in incubator and carries out height by the following conditions
Temperature processing 1~4 day:
When 8 whens of the morning~afternoon 6,45 DEG C；
When 6 whens of afternoon~the next morning 8,32 DEG C；
Simultaneously the cultivating seedling of room temperature control group is placed in the incubator of another same model, and with high-temperature process group phase
Same intensity of illumination, light application time, humidity and CO2Concentration, temperature is controlled at 32 DEG C, lasting to cultivate day identical with high-temperature process
Number；
(3) after treatment takes several strains of cultivating seedling, room temperature by same rice varieties in each culture dish of high-temperature process group
Control group also chooses the cultivating seedling of identical strain number in the same way；
(4) it will be sequentially placed into root system scanning system and be scanned behind the clean root of the cultivating seedling of acquirement, counted respectively by kind
The white root of room temperature control group and the white root of high-temperature process group；
(5) heat-resistance coefficient of different cultivars is calculated as follows:
Heat-resistance coefficient=(white root of room temperature control group-high-temperature process group white root)/room temperature control group white root ×
100%
Kind heat resistance difference is determined from high to low with heat-resistance coefficient, and the higher kind of heat-resistance coefficient, heat resistance is better.
2. according to the method described in claim 1, it is characterized by: in step (1), by the kind of the sprouting after seed soaking disinfection
Son, uniform pendulum are covered in the culture dish of blotting paper in diameter 20cm, and the grain number sowed in each culture dish is 40, are carried out
Water planting, the culture dish that each rice varieties carry out water planting is 12, wherein 6 are high-temperature process group, remaining 6 are room temperature control
Group.
3. according to the method described in claim 2, it is characterized by: in step (3), after treatment, each rice varieties
The each culture dish of high-temperature process group in take intermediate 10 plants of cultivating seedling, 6 culture dishes take 60 plants of cultivating seedling altogether, room temperature control group
Also 60 plants of cultivating seedlings are chosen in the same way.
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| CN109644844B (en) * | 2018-12-21 | 2021-06-18 | 安徽省农业科学院水稻研究所 | Method for screening drought-tolerant variety by using white root number of rice |
| CN114521459A (en) * | 2022-02-22 | 2022-05-24 | 安徽国瑞种业有限公司 | Method for cultivating heat-resistant disease-resistant nitrogen efficient rice |
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| CN102160510A (en) * | 2010-12-24 | 2011-08-24 | 李泽福 | Method for screening strong heat-resisting material in rice heading and flowering stage |
| CN102487771A (en) * | 2011-12-28 | 2012-06-13 | 宁波市农业科学研究院 | Heading stage high-temperature resistance identifying method for rice variety |
| CN103828666B (en) * | 2014-03-07 | 2016-02-24 | 四川省农业科学院水稻高粱研究所 | The field identification method of rice varieties heat-resisting quantity in flowering stage |
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