CN105532394A - Method for screening rice heat-resisting variety by using white root number in seedling stage - Google Patents
Method for screening rice heat-resisting variety by using white root number in seedling stage Download PDFInfo
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- CN105532394A CN105532394A CN201510908336.2A CN201510908336A CN105532394A CN 105532394 A CN105532394 A CN 105532394A CN 201510908336 A CN201510908336 A CN 201510908336A CN 105532394 A CN105532394 A CN 105532394A
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- high temperature
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a method for screening a rice heat-resisting variety by using white root number in a seedling stage. The method comprises: performing hydroponics on different rice varieties to four-leaf stage, performing high temperature processing, after processing is finished, detecting differences of white root number of different variety roots, calculating a heat-resisting coefficient, determining heat resistance difference of different varieties, so as to rapidly screen the heat-resisting variety. The method uses a seedling stage root index to screen the rice heat-resisting variety, and can screen hear resistance of rice varieties indoors in an early stage, and saves large amount of manpower and material resources, and target is more obvious.
Description
Technical field
The present invention relates to a kind of method utilizing white root screening in seedling stage paddy rice heat resistant variety.
Background technology
Along with the trend of global warming is more and more serious, high temperature also obtains the attention of people day by day to the harm of crop, up to the present, fuzzyyer to the understanding of the Climatic regionalization mechanism of action, and this is also for reply heat evil in production adds difficulty.China In Middle And Lower Reaches of Changjiang River is the severely afflicated area of paddy rice high temperature damage, and in recent years, high temperature damage is more and more serious, and particularly along with the increase day by day of the popularizing area of hybrid rice, heat evil has threatened the production safety in this important goods grain base, the Yangtze river basin.Practice shows that the high temperature resistant kind of seed selection is the effective means solving paddy rice Climatic regionalization.
The screening of current paddy rice heat resistant variety makes some progress, and has filtered out a collection of heat resistant variety for breeding practice, but the method still existing defects of heat resistant variety screening at present, ignore the under ground portion of paddy rice and root system selection index system.We find in research is carried out, and seedling stage, there are differences of different its root system of heat resistant variety, with this, we have proposed a kind of white root that utilizes for the method for screening index screening paddy rice heat resistant variety to paddy rice high temperature treatment.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method utilizing white root screening in seedling stage paddy rice heat resistant variety.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is, utilizes the method for white root screening in seedling stage paddy rice heat resistant variety, comprises the following steps:
(1) by the seed of same rice varieties by the sprouting after many seed soaking sterilizations, evenly be placed in even number culture dish and carry out water planting, and using the half of the even number culture dish of this rice varieties as high temperature treatment group, second half culture dish is as room temperature control group;
(2) until in culture dish during water-culturing rice to the 4 leaf phase, high temperature treatment group is placed in incubator and carries out high temperature treatment 1 ~ 4 day by following condition:
During 8 times of the morning ~ afternoon 6,45 DEG C;
During 6 times of afternoon ~ the next morning 8,32 DEG C;
The cultivation seedling of room temperature control group is placed in the incubator of another one same model simultaneously, and the intensity of illumination identical with high temperature treatment group, light application time, humidity and CO
2concentration, temperature controls at 32 DEG C, continues to cultivate the number of days identical with high temperature treatment;
(3), after process terminates, get in each culture dish of high temperature treatment group by same rice varieties and cultivate the some strains of seedling, room temperature control group also chooses the cultivation seedling of same strain number in the same way;
(4) put into root system scanning system successively after the cultivation seedling obtained being cleaned root to scan, add up the white root of room temperature control group and the white root of high temperature treatment group respectively by kind;
(5) heat-resistance coefficient of different cultivars is calculated as follows:
The white root of the white root-high temperature treatment group of heat-resistance coefficient=room temperature control group) white root × 100% of/room temperature control group
Determine kind warm tolerance difference from high to low with heat-resistance coefficient, the kind that heat-resistance coefficient is higher, warm tolerance is better.
As preferably, in step (1), by the seed of the sprouting after seed soaking sterilization, uniform pendulum, at diameter 20cm, is covered with in the culture dish of blotting paper, and the grain number sowed in each culture dish is 40, carry out water planting, the culture dish that each rice varieties carries out water planting is 12, and wherein 6 is high temperature treatment group, and all the other 6 is room temperature control group.
As preferably, in step (3), after process terminates, in each culture dish of high temperature treatment group of each rice varieties, get middle cultivation seedling 10 strain, 6 culture dishes are got altogether and are cultivated seedling 60 strain, and room temperature control group is also chosen 60 strains in the same way and cultivated seedling.
The invention has the beneficial effects as follows:
1, utilize Seedling root index screening paddy rice heat resistant variety, can screen rice varieties warm tolerance in early days in indoor, save a large amount of man power and materials, Objective is more obvious.
2, adopt white root to be that index carries out heat resistant variety screening by the mode of water planting, the number of tested variety can be increased, shorten the cycle of paddy rice warm tolerance screening, thus greatly can accelerate speed and the accuracy of heat resistant variety screening.
3, utilize indoor white root screening in seedling stage heat resistant variety, avoid the problem that experimental result repeatability that is that cause is poor with ripening rate screening heat resistant variety is inconsistent by different year weather conditions, further ensure the reliability of heat resistant variety screening.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the situation of change that the present invention utilizes different cultivars 2 kinds of heat-resistance coefficient of the embodiment of the method for white root screening in seedling stage paddy rice heat resistant variety.
Mark in figure:
1) 1-17 represents following 17 rice varieties respectively: BALILLA, DULAR, CR547-1-2-3, CUIABANA, GIZA178, GZ948-2-2-1, IDSA77, IR2307-247-2-2-3, IR1561-228-3-3, IR36, IR50, IR60, KCN80152, N22, SAKHA104, TCHAMPA, WAB56-125, WAB96-1-1.
2) series 1 is the heat-resistance coefficient based on white root, and series 2 is the heat-resistance coefficient based on ripening rate.
Embodiment
Select 17 the different heat resistant varieties utilizing ripening rate to filter out as heat-resistance coefficient: BALILLA, DULAR, CR547-1-2-3, CUIABANA, GIZA178, GZ948-2-2-1, IDSA77, IR2307-247-2-2-3, IR1561-228-3-3, IR36, IR50, IR60, KCN80152, N22, SAKHA104, TCHAMPA, WAB56-125, WAB96-1-1, seedling stage water planting, after 4 leaf phase high temperature treatment, detect white root, calculate heat-resistance coefficient as follows:
White root × 100% of heat-resistance coefficient=(white root of the white root-high temperature treatment group of room temperature control group)/room temperature control group
With the above-mentioned heat-resistance coefficient calculated for Testing index, analyze the warm tolerance of different heat resistant variety, result shows, use the heat-resistance coefficient based on white root basically identical with the heat-resistance coefficient variation tendency based on ripening rate, the warm tolerance difference (Fig. 1) of rice varieties can be screened.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.
Claims (3)
1. utilize the method for white root screening in seedling stage paddy rice heat resistant variety, comprise the following steps:
(1) by the seed of same rice varieties by the sprouting after many seed soaking sterilizations, evenly be placed in even number culture dish and carry out water planting, and using the half of the even number culture dish of this rice varieties as high temperature treatment group, second half culture dish is as room temperature control group;
(2) until in culture dish during water-culturing rice to the 4 leaf phase, high temperature treatment group is placed in incubator and carries out high temperature treatment 1 ~ 4 day by following condition:
During 8 times of the morning ~ afternoon 6,45 DEG C;
During 6 times of afternoon ~ the next morning 8,32 DEG C;
The cultivation seedling of room temperature control group is placed in the incubator of another one same model simultaneously, and the intensity of illumination identical with high temperature treatment group, light application time, humidity and CO
2concentration, temperature controls at 32 DEG C, continues to cultivate the number of days identical with high temperature treatment;
(3), after process terminates, get in each culture dish of high temperature treatment group by same rice varieties and cultivate the some strains of seedling, room temperature control group also chooses the cultivation seedling of same strain number in the same way;
(4) put into root system scanning system successively after the cultivation seedling obtained being cleaned root to scan, add up the white root of room temperature control group and the white root of high temperature treatment group respectively by kind;
(5) heat-resistance coefficient of different cultivars is calculated as follows:
The white root of the white root-high temperature treatment group of heat-resistance coefficient=room temperature control group) white root × 100% of/room temperature control group
Determine kind warm tolerance difference from high to low with heat-resistance coefficient, the kind that heat-resistance coefficient is higher, warm tolerance is better.
2. method according to claim 1, it is characterized in that: in step (1), by the seed of the sprouting after seed soaking sterilization, uniform pendulum, at diameter 20cm, is covered with in the culture dish of blotting paper, and the grain number sowed in each culture dish is 40, carry out water planting, the culture dish that each rice varieties carries out water planting is 12, and wherein 6 is high temperature treatment group, and all the other 6 is room temperature control group.
3. method according to claim 2, it is characterized in that: in step (3), after process terminates, middle cultivation seedling 10 strain is got in each culture dish of high temperature treatment group of each rice varieties, 6 culture dishes are got altogether and are cultivated seedling 60 strain, and room temperature control group is also chosen 60 strains in the same way and cultivated seedling.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644844A (en) * | 2018-12-21 | 2019-04-19 | 安徽省农业科学院水稻研究所 | A method of drought-enduring variety is screened using rice white root |
| CN114521459A (en) * | 2022-02-22 | 2022-05-24 | 安徽国瑞种业有限公司 | Method for cultivating heat-resistant disease-resistant nitrogen efficient rice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160510A (en) * | 2010-12-24 | 2011-08-24 | 李泽福 | Method for screening strong heat-resisting material in rice heading and flowering stage |
| CN102487771A (en) * | 2011-12-28 | 2012-06-13 | 宁波市农业科学研究院 | Heading stage high-temperature resistance identifying method for rice variety |
| CN103828666A (en) * | 2014-03-07 | 2014-06-04 | 四川省农业科学院水稻高粱研究所 | Filed identification method of flowering stage high temperature resistance of rice varieties |
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2015
- 2015-12-10 CN CN201510908336.2A patent/CN105532394B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160510A (en) * | 2010-12-24 | 2011-08-24 | 李泽福 | Method for screening strong heat-resisting material in rice heading and flowering stage |
| CN102487771A (en) * | 2011-12-28 | 2012-06-13 | 宁波市农业科学研究院 | Heading stage high-temperature resistance identifying method for rice variety |
| CN103828666A (en) * | 2014-03-07 | 2014-06-04 | 四川省农业科学院水稻高粱研究所 | Filed identification method of flowering stage high temperature resistance of rice varieties |
Non-Patent Citations (4)
| Title |
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| 吕俊等: "水杨酸对高温胁迫下水稻幼苗抗热性的影响", 《中国生态农业学报》 * |
| 宋丽莉等: "水稻幼苗获得性耐热性研究", 《安徽农业科学》 * |
| 杨波等: "水分和高温复合胁迫对水稻幼苗光合生理特性的影响", 《作物杂志》 * |
| 许红云等: "高温对粳稻品种发芽及幼苗生长发育影响的研究", 《西南农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109644844A (en) * | 2018-12-21 | 2019-04-19 | 安徽省农业科学院水稻研究所 | A method of drought-enduring variety is screened using rice white root |
| CN114521459A (en) * | 2022-02-22 | 2022-05-24 | 安徽国瑞种业有限公司 | Method for cultivating heat-resistant disease-resistant nitrogen efficient rice |
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