CN105520971A - New uses of pollia japonica in preparation of medicines treating and/or preventing hyperlipidemia - Google Patents
New uses of pollia japonica in preparation of medicines treating and/or preventing hyperlipidemia Download PDFInfo
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- A61K2236/30—Extraction of the material
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- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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Abstract
The invention relates to new uses of pollia japonica, namely new uses of pollia japonica in preparation of medicines treating and/or preventing hyperlipidemia, particularly new uses of a pollia japonica water extract and a pollia japonica alcohol extract in preparation of medicines treating and/or preventing hyperlipidemia.
Description
Technical field
The present invention relates to the novelty teabag of Rhizoma Polliae Japonicae, particularly the novelty teabag of Rhizoma Polliae Japonicae water extract and Rhizoma Polliae Japonicae ethanol extract, namely prepare the novelty teabag treated and/or prevented in hyperlipidemia.
Background technology
Hyperlipemia is the main inducing of various cardiovascular disease, the infringement of this disease to health be concealment, gradually, Progressive symmetric erythrokeratodermia and general.Along with rising and the prolongation of dyslipidemia time of blood fat, it can cause the various diseases such as atherosclerosis, coronary heart disease, hypertension, diabetes, apoplexy, fatty liver.The sickness rate of these cardiovascular and cerebrovascular diseases is high, and harm is large, and disease progression is dangerous, about its mortality rate accounts for the half of mankind's general mortality rate.According to the display of epidemiology statistics data, the normal population that the probability suffering from hyperlipidemia population morbidity was not organized than the same age is high, and its sickness rate will exceed 4 times.
Along with improving constantly of people's living standard, dyslipidemia also will present obvious growth trend.Hyperlipemia sickness rate in the middle of middle-aged and elderly people is high, and along with China steps into aging society, the sickness rate of hyperlipemia increases day by day, becomes an important content in China's Healthcare gradually.The continuous increase of hyperlipidemia population, and people are to the raising of its recognizing dangers, constantly expand, attract people's attention to the demand of blood lipid-lowering medicine, blood lipid-lowering medicine remains the quick growing trend of annual 9%.Therefore, research and develop novel blood lipid-lowering medicine and there is great Social benefit and economic benefit.
At present, the medicine being used for the treatment of dyslipidemia clinically comprises: Statins, shellfish special class, nicotinic acid class, resinae, cholesterol absorption inhibitor and antioxidant.But these medicines all have side effect in various degree, especially calendar year 2001 visit sthene in 1997 listing after, the whole world has 52 examples and visits sthene cause rhabdomyolysis, destroyed muscle cell to cause the exhaustion of renal function thus the report of causing death by renal excretion because taking, the generation of all untoward reaction, the untoward reaction of people to Western medicine is worried more, patient dependence declines, and have impact on the normal therapeutic of hyperlipidemia patient.
But the side effect for the treatment of by Chinese herbs disease is little, and untoward reaction is few, compared with Western medicine more compatriots accepted.Though the name of disease of traditional Chinese medical science ahyperlipoidemia, the cause of disease produced according to hyperlipemia, pathogenic characteristic and associated diseases, the expectorant that can be summarized as the traditional Chinese medical science is turbid, blood stasis, carries out determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs.
Rhizoma Polliae Japonicae is usually used in treatment for lumbago, traumatic injury.Herb: regulating QI to relieve pain, dispelling wind and eliminating swelling.Have a pain for the stagnation of QI, skin swells and ache, stomachache, stranguria; Outer for snake bite and insect sting, carbuncle furuncle furuncle, proctoptosis." China National medicine will will " points out this herb also for controlling wind-eye, and dizzy dizzy, lumbago and skelalgia, does not have documents and materials to mention Rhizoma Polliae Japonicae, Rhizoma Polliae Japonicae water extract or Rhizoma Polliae Japonicae ethanol extract at present and can be used for treating and/or preventing hyperlipemia.
Summary of the invention
The object of this invention is to provide Rhizoma Polliae Japonicae and prepare the novelty teabag treated and/or prevented in hyperlipidemia;
Another object of the present invention is to provide Rhizoma Polliae Japonicae water extract and is preparing the novelty teabag treated and/or prevented in hyperlipidemia;
Another object of the present invention is to provide the preparation method of Rhizoma Polliae Japonicae water extract;
Another object of the present invention is to provide Rhizoma Polliae Japonicae ethanol extract and is preparing the novelty teabag treated and/or prevented in hyperlipidemia;
Another object of the present invention is to provide the preparation method of Rhizoma Polliae Japonicae ethanol extract.
The object of the invention is by following technical scheme realize:
Raw material sources of the present invention:
Rhizoma Polliae Japonicae: be the dry herb of Commelianaceae plant Rhizoma Polliae Japonicae PolliajaponicaThunb..Pungent, tepor.Root stock: the kidney invigorating.Swollen.
Inventor finds in the therapeutic process of research hyperlipemia, and Rhizoma Polliae Japonicae has reduction TC, TG and LDL and raises the effect of HDL simultaneously, thus effectively can treat hyperlipemia.The safety of patent clinical practice of the present invention is higher, a kind of good medicine of can yet be regarded as in the Drug therapy for the treatment of hyperlipemia, and it has widened the new method for the treatment of hyperlipemia, the untoward reaction simultaneously for occurring when patient solves prior art treatment hyperlipemia.
Therefore, applicant provide Rhizoma Polliae Japonicae, Rhizoma Polliae Japonicae water extract, Rhizoma Polliae Japonicae ethanol extract be used for the treatment of/or prevention hyperlipidemia aspect novelty teabag.
The invention also discloses Rhizoma Polliae Japonicae water extract treats and/or prevents hyperlipidemia application in preparation, the preparation of described Rhizoma Polliae Japonicae water extract comprises the following steps:
Get Rhizoma Polliae Japonicae, add medical material weight 5-7 times water gaging, boil, under agitation slowly add lime cream to PH8-9, under the condition of PH8-9, keep micro-30min that boils, sucking filtration while hot, residue adds a medical material weight 3-5 times decocting again and carries 1-3 time, each decoction 1-3 hour, sucking filtration while hot, merge extractive liquid, under 60-70 DEG C of condition, adjust filtrate PH to 5.0-5.5 with the hydrochloric acid of 36%-38%, stir evenly rear standing 24h, sucking filtration, it is 6.5-7.5 that precipitate is washed to PH, and 55 DEG C of-75 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae water extract.
Rhizoma Polliae Japonicae water extract disclosed by the invention treats and/or prevents the application of hyperlipidemia in preparation, and the preparation of described Rhizoma Polliae Japonicae water extract is preferably following steps:
Get Rhizoma Polliae Japonicae, add medical material weight 6 times of water gagings, boil, under agitation slowly add lime cream to PH8.5, under the condition of PH8.5, keep micro-30min that boils, sucking filtration while hot, residue adds medical material weight 4 times of decoctings again and carries 2 times, each decoction 1.5 hours, sucking filtration while hot, merges extracted twice liquid, under 65 DEG C of conditions, 37% hydrochloric acid adjusts filtrate PH to 5.3, stirs evenly rear standing 24h, sucking filtration, precipitate washing PH is 7.0,60 DEG C of vacuum dryings, obtains Rhizoma Polliae Japonicae water extract.
The invention also discloses Rhizoma Polliae Japonicae ethanol extract treats and/or prevents hyperlipidemia application in preparation, the preparation of described Rhizoma Polliae Japonicae ethanol extract comprises the following steps:
Get Rhizoma Polliae Japonicae, adding concentration is 45%-65% ethanol extraction 1-3 time, adds medical material weight 4-9 45%-65% alcohol heating reflux doubly at every turn and extracts, and each extraction 1-3 hour, merge extractive liquid, filters, and filtrate concentrates, and 55 DEG C of-75 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
Rhizoma Polliae Japonicae ethanol extract disclosed by the invention treats and/or prevents the application of hyperlipidemia in preparation, and the preparation of described Rhizoma Polliae Japonicae ethanol extract is preferably following steps:
Get Rhizoma Polliae Japonicae, adding concentration is 50% ethanol extraction 3 times, 50% alcohol heating reflux that first time adds medical material weight 8 times extracts 2 hours, second time adds 50% ethanol extraction 2 hours of medical material weight 7 times, and third time adds 50% ethanol extraction 1 hour of medical material weight 4 times, merge extractive liquid, filter, filtrate concentrates, and 60 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
Rhizoma Polliae Japonicae provided by the invention, Rhizoma Polliae Japonicae water extract, Rhizoma Polliae Japonicae ethanol extract be used for the treatment of/or the novelty teabag of prevention hyperlipidemia aspect have the following advantages:
1, Rhizoma Polliae Japonicae, Rhizoma Polliae Japonicae water extract, Rhizoma Polliae Japonicae ethanol extract obtain, safety non-toxic from labiate Rabdosia lophanthoides plant Rhizoma Polliae Japonicae, can long-term taking.
2, animal data shows, medicine of the present invention is compared with hyperlipidemia model group, blood lipid level obviously reduces (P < 0.01 or P < 0.05), and significantly reduce TC, TG, LDL level in rat blood serum, raising HDL levels (P < 0.05 or P < 0.01), wherein Rhizoma Polliae Japonicae 50% ethanol extract, Rhizoma Polliae Japonicae 65% ethanol extract difference very remarkable (P < 0.01) compared with hyperlipidemia model group.Illustrate that Rhizoma Polliae Japonicae water extract and ethanol extract all have effect for reducing blood fat, wherein 50% ethanol extraction, 65% ethanol extraction lipid-lowering effect optimum.
3, the extract Bloodlipid-lowering prepared with Rhizoma Polliae Japonicae is remarkable, and technique is simple, and Extraction solvent ethanol can recycle and reuse, and integrated cost is low, is a kind of novel lipid lowerers that can be used for long-term lipid-lowering therapy.
Detailed description of the invention
The present invention is further illustrated below by embodiment.It should be understood that embodiments of the invention are for illustration of the present invention instead of limitation of the present invention.Essence according to the present invention all belongs to the scope of protection of present invention to the simple modifications that the present invention carries out.Except as otherwise noted, the percent of the amount of alcohol in the present invention is percentage by volume, and v/v represents the volume ratio of solution.
Embodiment 1:
Get Rhizoma Polliae Japonicae 10kg, add medical material weight 5 times of water gagings, boil, under agitation slowly add lime cream to PH8, under the condition of PH8, keep micro-30min that boils, sucking filtration while hot, residue adds medical material weight 3 times of decoctings again and carries 1 time, decocts 1 hour, sucking filtration while hot, under 60 DEG C of conditions, the hydrochloric acid with 36% adjusts filtrate PH to 5.0, stir evenly rear standing 24h, sucking filtration, it is 6.5 that precipitate is washed to PH, 55 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae water extract.
Embodiment 2:
Get Rhizoma Polliae Japonicae 10kg, add medical material weight 6 times of water gagings, boil, under agitation slowly add lime cream to PH8.5, under the condition of PH8.5, keep micro-30min that boils, sucking filtration while hot, residue adds medical material weight 4 times of decoctings again and carries 2 times, each decoction 1.5 hours, sucking filtration while hot, merges extracted twice liquid, under 65 DEG C of conditions, 37% hydrochloric acid adjusts filtrate PH to 5.3, stirs evenly rear standing 24h, sucking filtration, it is 7.0,60 DEG C of vacuum dryings that precipitate is washed to PH, obtains Rhizoma Polliae Japonicae water extract.
Embodiment 3:
Get Rhizoma Polliae Japonicae 10kg, add medical material weight 7 times of water gagings, boil, under agitation slowly add lime cream to PH9, under the condition of PH9, keep micro-30min that boils, sucking filtration while hot, residue adds medical material weight 5 times of decoctings again and carries 3 times, each decoction 3 hours, sucking filtration while hot, merge extractive liquid, under 60-70 DEG C of condition, hydrochloric acid with 38% adjusts filtrate PH to 5.5, stirs evenly rear standing 24h, sucking filtration, it is 7.5,70 DEG C of vacuum dryings that precipitate is washed to PH, obtains Rhizoma Polliae Japonicae water extract.
Embodiment 4:
Get Rhizoma Polliae Japonicae 10kg, adding concentration is 45% alcohol reflux 2 times, 45% ethanol that first time adds medical material weight 9 times, extracts 3 hours, second time adds 45% ethanol of medical material weight 6 times, extracts 2 hours, filters, filtrate concentrates, and 55 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
Embodiment 5:
Get Rhizoma Polliae Japonicae 10kg, adding concentration is 50% ethanol extraction 3 times, 50% alcohol heating reflux that first time adds medical material weight 8 times extracts 2 hours, second time adds 50% ethanol extraction 2 hours of medical material weight 7 times, and third time adds 50% ethanol extraction 1 hour of medical material weight 4 times, merge extractive liquid, filter, filtrate concentrates, and 60 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
Embodiment 6:
Get Rhizoma Polliae Japonicae 10kg, adding concentration is 65% ethanol extraction 3 times, 65% alcohol heating reflux that first time adds medical material weight 7 times extracts 3 hours, second time adds 65% ethanol extraction 2 hours of medical material weight 6 times, and third time adds 50% ethanol extraction 1 hour of medical material weight 4 times, merge extractive liquid, filter, filtrate concentrates, and 75 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
Effect experiment:
One, Rhizoma Polliae Japonicae extract is on the impact of acute hyperlipemia level
1. experiment material
(1) animal
SPF level Kun Ming mice, robust half and half, body weight 20 ± 2g, buy from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
(2) medicine and instrument
Embodiment of the present invention 1-6 group, 0.9% normal saline (Pharmaceutical Group Co., Ltd of Nanning Baihui), fenofibrate (SIGMA company), automatic clinical chemistry analyzer (manufacturer: step auspicious medical international corporation, model: BS-190).
2. experimental technique
Select healthy kunming mice 80, male and female half and half, be divided into 8 groups at random: blank, model comparison, positive controls, embodiment 1-5 group, often organize 10.
Each group of continuous gavage gives tested material 11d, gavage volume is 0.2ml/10g, positive controls gives fenofibrate, dosage is 100mg/kg, embodiment 1-6 group dosage is 150mg/kg, test 10d except blank group, all the other are respectively organized lumbar injection 75% egg yolk Emulsion (75% egg yolk normal saline suspension) 0.1mL/10g and carry out modeling.Before last gavage, water 12h is can't help in animal fasting, and after last gavage, 1h mice is plucked eyeball and gets blood, and the centrifugal 4min separation of serum of 3000r/min measures TC, TG value.
3. experimental result
The each tested material of table 1 on the impact of hyperlipidemia model lipid of mice level caused by Ovum Gallus domesticus album Emulsion (
n=10)
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with model control group, #P < 0.05, ##P < 0.01.
Table 1 result shows: acute hyperlipidemia model group mice serum T-CHOL and triglyceride are higher than blank group, and difference very significantly (P < 0.01); Positive controls, embodiment group are compared with hyperlipidemia model group, blood lipid level obviously reduces (P < 0.01 or P < 0.05), wherein the present invention 50% ethanol extract, 65% ethanol extract difference very remarkable (P < 0.01) compared with hyperlipidemia model group.Illustrate that Rhizoma Polliae Japonicae water extract and ethanol extract have certain effect for reducing blood fat, wherein 50% ethanol extraction, 65% ethanol extraction lipid-lowering effect optimum.
Two, Rhizoma Polliae Japonicae extract is on the impact of Diet hyperlipemia blood lipid level.
1, experiment material
(1) animal
SD rat, male, body weight 180 ~ 210g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
(2) medicine and instrument
Embodiment of the present invention 1-5 group, 0.9% normal saline (Pharmaceutical Group Co., Ltd of Nanning Baihui), XUEZHIKANG JIAONANG (Beijing WBL Peking University Biotech Co., Ltd), automatic clinical chemistry analyzer (manufacturer: step auspicious medical international corporation, model: BS-190).
2, experimental technique
(1) the modeling phase: select healthy SD rat 80, male, rat is divided at random blank group (10), all the other are high fat group, two groups of rat body weight there was no significant difference (t=0.1132, P > 0.05), blank group gives normal diet, and high fat group gives high lipid food (79% normal feedstuff+1% cholesterol+10% yolk powder+10% Adeps Sus domestica).After 4 weeks, blank group and high fat group are randomly drawed 10 animals and are got orbital vein hematometry serum TC, TG, HDL, LDL, and as testing result height fat group is significantly higher than blank group, then rat hyperlipidemia model is formed.The results are shown in Table 2.
(2) experiment periods: be divided at random by 70 of high lipid food of feeding rats: model group, embodiment 1-6 group and comparative example group, often organizes 10 animals.The weight differences not statistically significant (F=0.2681, P > 0.05) of 7 groups of rats.
Within 5 weeks, be experiment periods after experiment, except blank group, all the other 7 treated animals continue high lipid food of feeding, embodiment 1-6 group and comparative example group press scheme gavage every day, embodiment 1-6 group dosage is 150mg/kg, and comparative example group gives Xuezhikang, and dosage is 250mg/kg, model group and the isopyknic distilled water of blank group gavage, gavage volume is 10ml/kg.At the end of experiment, Rat Fast can't help water 12h.Broken end gets blood, surveys the content of serum lipids.The results are shown in Table 3.
3, experimental result
Table 2 blank group and high fat group rat modeling phase lipids detection result
Note: compare with blank group, * * P < 0.01.
Table 2 result shows: TC, TG, HDL, LDL value of high fat group is all higher than blank group, and significant difference (P < 0.01), illustrates rat hyperlipidemia model modeling success.
The each tested material of table 3 on the impact of rat fat (
n=10)
Note: compare with blank group, * P < 0.05, * * P < 0.01; Compare with model control group, #P < 0.05, ##P < 0.01.
Table 2 result show: TC, TG, LDL value of high fat group all higher than blank group, HDL value lower than blank group, significant difference (P < 0.01).Compared with hyperlipidemia model group, comparative example group and embodiment group all significantly reduce TC, TG, LDL level in rat blood serum, raising HDL levels (P < 0.05 or P < 0.01), wherein Rhizoma Polliae Japonicae 50% ethanol extraction, Rhizoma Polliae Japonicae 65% ethanol extraction difference very remarkable (P < 0.01) compared with hyperlipidemia model group.Illustrate that Rhizoma Polliae Japonicae extract has significant effect for reducing blood fat, wherein 50% ethanol extraction, 65% ethanol extraction lipid-lowering effect optimum.
Experimental result shows: Rhizoma Polliae Japonicae water extract of the present invention and ethanol extract effectively can reduce the blood lipid level of hyperlipidemia model animal, wherein 50% ethanol extraction, 65% ethanol extraction lipid-lowering effect optimum.
4, discuss
Serum total cholesterol (TC), triglyceride (TG), low density lipoprotein, LDL (LDL) is higher all can cause hyperlipemia, and high density lipoprotein (HDL) is the base substance of blood lipid metabolism, it is a kind of antiatherogenic plasma lipoprotein, there is the effect of removing the unnecessary blood fat of Ink vessel transfusing, removing blood dirt, cleaning blood vessel, be commonly called as " blood vessel street cleaner ".Animal data shows, medicine of the present invention is compared with hyperlipidemia model group, blood lipid level obviously reduces (P < 0.01 or P < 0.05), and significantly reduce TC, TG, LDL level in rat blood serum, raising HDL levels (P < 0.05 or P < 0.01), wherein Rhizoma Polliae Japonicae 50% ethanol extract, Rhizoma Polliae Japonicae 65% ethanol extract difference very remarkable (P < 0.01) compared with hyperlipidemia model group.Illustrate that Rhizoma Polliae Japonicae water extract and ethanol extract all have effect for reducing blood fat, wherein 50% ethanol extraction, 65% ethanol extraction lipid-lowering effect optimum.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (9)
1. Rhizoma Polliae Japonicae is preparing the novelty teabag treated and/or prevented in hyperlipidemia.
2. Rhizoma Polliae Japonicae water extract is preparing the novelty teabag treated and/or prevented in hyperlipidemia.
3. purposes according to claim 2, it is characterized in that described Rhizoma Polliae Japonicae water extract is prepared by following steps and obtains: get Rhizoma Polliae Japonicae, add medical material weight 5-7 times water gaging, boil, under agitation slowly add lime cream to PH8-9, under the condition of PH8-9, keep micro-30min that boils, sucking filtration while hot, residue adds a medical material weight 3-5 times decocting again and carries 1-3 time, each decoction 1-3 hour, sucking filtration while hot, merge extractive liquid, under 60-70 DEG C of condition, filtrate PH to 5.0-5.5 is adjusted with the hydrochloric acid of 36%-38%, stir evenly rear standing 24h, sucking filtration, it is 6.5-7.5 that precipitate is washed to PH, 55 DEG C of-75 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae water extract.
4. purposes according to claim 3, it is characterized in that described Rhizoma Polliae Japonicae water extract is prepared by following steps and obtains: get Rhizoma Polliae Japonicae, add medical material weight 6 times of water gagings, boil, under agitation slowly add lime cream to PH8.5, under the condition of PH8.5, keep micro-30min that boils, sucking filtration while hot, residue adds medical material weight 4 times of decoctings again and carries 2 times, each decoction 1.5 hours, sucking filtration while hot, merge extracted twice liquid, under 65 DEG C of conditions, 37% hydrochloric acid adjusts filtrate PH to 5.3, stir evenly rear standing 24h, sucking filtration, precipitate washing PH is 7.0, 60 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae water extract.
5. Rhizoma Polliae Japonicae ethanol extract is preparing the novelty teabag treated and/or prevented in hyperlipidemia.
6. purposes according to claim 5, it is characterized in that described Rhizoma Polliae Japonicae ethanol extract is prepared by following steps and obtains: get Rhizoma Polliae Japonicae, adding concentration is 45%-65% ethanol extraction 3 times, add medical material weight 4-9 45%-65% alcohol heating reflux doubly to extract, each extraction 1-3 hour, merge extractive liquid, at every turn, filter, filtrate concentrates, and 55 DEG C of-75 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
7. purposes according to claim 5, it is characterized in that described Rhizoma Polliae Japonicae ethanol extract is prepared by following steps and obtains: get Rhizoma Polliae Japonicae, adding concentration is 50% ethanol extraction 3 times, and 50% alcohol heating reflux that first time adds medical material weight 8 times extracts 2 hours, and second time adds 50% ethanol extraction 2 hours of medical material weight 7 times, third time adds 50% ethanol extraction 1 hour of medical material weight 4 times, merge extractive liquid, filters, and filtrate concentrates, 60 DEG C of vacuum dryings, obtain Rhizoma Polliae Japonicae ethanol extract.
8. Rhizoma Polliae Japonicae water extract and pharmaceutically acceptable carrier combination are prepared into the novelty teabag treating and/or preventing hyperlipidemia aspect.
9. Rhizoma Polliae Japonicae ethanol extract and pharmaceutically acceptable carrier combination are prepared into the novelty teabag treating and/or preventing hyperlipidemia aspect.
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Application publication date: 20160427 |