CN105497976A - Preparation method of externally applied functional ointment for traumatic wounds - Google Patents
Preparation method of externally applied functional ointment for traumatic wounds Download PDFInfo
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- CN105497976A CN105497976A CN201610008247.7A CN201610008247A CN105497976A CN 105497976 A CN105497976 A CN 105497976A CN 201610008247 A CN201610008247 A CN 201610008247A CN 105497976 A CN105497976 A CN 105497976A
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- sodium alginate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0004—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/45—Mixtures of two or more drugs, e.g. synergistic mixtures
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
Abstract
The invention discloses a preparation method of an externally applied functional ointment for traumatic wounds. The method comprises the steps that marine biological resource sodium alginate and modified chitin are taken as the raw materials, an ionic bonding reaction, a silver ion complexing reaction and a nano-silver reaction are conducted, a natural antibacterial enhancer capable of moistening and repairing skin injuries and a nano-silver anti-photosensitivity agent are added, and the externally applied functional ointment, namely, a silver liquid band-aid product with a golden appearance, for the traumatic wounds is prepared, wherein the functional ointment is capable of meeting an emergency, convenient to use, rapid in film formation, rapid in hemostasis and sterilization and capable of bonding, relieving itching and moistening and repairing the skin injuries. The prepared product can serve as a liquid band-aid, has the effects of stopping bleeding, relieving itching, repairing skin, promoting healing and reducing scars and has the advantages of being rapid in film formation, easy to remove, free of off-flavors, suitable for hemostasis and sterilization of skin traumas and suitable for an adjuvant therapy of skin itching relieving or dermatophytosis or eczema and an adjuvant therapy of skin injury surfaces of scalds, burns and the like.
Description
Technical field
The present invention relates to the preparation method of the functional ointment of a kind of trauma wound external application, belong to Marine Biopharmaceutics field.
Background technology
People is in work and life, in motor process, it is unavoidable occurrence that limbs are subject to minor trauma.As processed not in time or when dealing with improperly, can occurring infect and leave obvious scar.So just there are numerous inventions of weapons medicine, vulnerary from ancient times, also have common people oneself to invent on wound by soil, by the emergent art such as plant ash, cuttlefish bone meal, certainly, success rate is low, how self-evident sequela is.
20 beginnings of the century, company has invented binder adhesive bandage Band-Aid (Bandaid) in Johnson & Johnson of the U.S. (J & J).Band refers to binder, and Aid is the meaning helping first aid.The adhesive bandage prototype in Here it is the current fashionable world.In Chinese market, this convenient and practical gizmo, is accustomed to the nursing of minor cut or wound owing to meeting Chinese, occupies more than half market of China at one stroke, and to calendar year 2001, Flexibi fabric bandages cumulative sale is more than 1,000 hundred million, and year creates market value 500,000,000 RMB.In adhesive bandage year slight at present, 1,000,000,000 yuans are reached in the market demand of China.
In China, pastille adhesive bandage has been invented on the basis of Bandaid by YUNNAN BAIYAO group, by 2007, the sales volume of Wound plaster of yannan Baiyao soars to nearly 200,000,000 yuan from 1,000 ten thousand of calendar year 2001 yuan, win the market of Chinese nearly half, create market value current year more than 400,000,000 yuans, run neck and neck with Bandaid.
Flexibi fabric bandages is actually by the strip adhesive plaster having elastic textile and rubber plaster adhesive and form, and has antiinflammatory, hemostasis, easy-to-use advantage.The pastille adhesive bandage of YUNNAN BAIYAO group invention adds the YUNNAN BAIYAO with century-brand, thus receives the accreditation in market.But they all belong to adhesive plaster adhesive bandage, textile material used, Polypropylence Sheet, binding agent are difficult to recycle, and contaminated environment, adhesive plaster easily causes skin allergy etc. to be all have the apparent shortcoming of binder adhesive bandage.
In recent years; liquid adhesive bandage has successively been invented by Octenisept company of Germany, Japanese holt company; can be used for scald, the emergency processing of skin Wicresoft wound that burn, cold injury, incised wound etc. cause; there is sterilization, stop blooding, relieve the pain, the effect such as promoting healing; also there is film forming water-proof function, use more convenient, be more conducive to environmental conservation; compensate for the deficiency of adhesive plaster adhesive bandage, also become the indispensable little medicine-chest member of welcome family at present.
But external liquid bandage contains the non-natural volatility medical adhesives such as a-cyanoacrylate class, not only there is the pungent taste of opaquing fluid, nial polish, and waterproof membrane is not easy to come off, need to soften with ethanol equal solvent, pain can be produced when down taking off, even hemorrhagely cause secondary injury.Secondly, the film of formation is because of airtight, and wound healing time is long, even occurs situation about infecting.
As Chinese patent application CN201110200972.1 disclose " a kind of liquid bandage and preparation method thereof ", its raw material comprises polyvinyl alcohol, dioctyl phthalate, glycerol, laurocapram etc., because of the polyvinyl alcohol containing difficult for biological degradation and toxic property volatility plasticiser dioctyl phthalate and become obvious defect.
Chinese patent application CN201310695850.3 discloses " a kind of self-crosslinking gel and its preparation method and application ", because making degradation under biocompatibility not enough containing poly(ethylene oxide), polyvinyl alcohol and aluminum salt Alumen.
Summary of the invention
The object of the invention is, the preparation method of the functional ointment of a kind of trauma wound external application is provided, it utilizes marine biomass source Sargassum polysaccharides and modification chitin polysaccharide, prepare nano silver gel, and add the natural medicinal ingredients such as hemostasis, antipruritic, antiinflammatory, skin modification, preparation has the liquid adhesive bandage of gold appearance feature.
The technical scheme that the present invention adopts for achieving the above object is, a kind of trauma wound external application preparation method of functional ointment, is characterized in that, comprise the following steps:
The first step is 1-5:1-10 in mass ratio, takes sodium alginate and modification chitin respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution that mass percent concentration is 1-5%; Modification chitin is placed in container, and adding distil water is mixed with the modification chitin aqueous solution that mass percent concentration is 1-10%;
Then, while stirring above-mentioned modification chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 60-90min, to make agreeing with and react completely therebetween, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 1-5 ︰ 1000, adds soluble silver salt in hygroscopicity supermolecule GCP, stirs 30-60min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent, control reaction temperature is 10-85 DEG C, and under stirring condition, reaction 20-60min; After question response is complete, then add the reducing agent of 0.1 mole, to make this reducing agent excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
Above-mentioned reducing agent is natural ascorbic acid, vanillin, or their mixture;
4th step, the ratio of 2%, 6% and 3% of antioxidation supermolecule nano silver colloid GCPAg quality is respectively in the quality of rose polyphenol, angelica keiskei koidzumi chalcone and squalene, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 1% rose wate alcohol extract, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion;
Golden yellow nanometer colloid base material is obtained after homogenizing; After golden yellow nanometer colloid base material packaging, product.
Be preferably, above-mentioned sodium alginate and modification chitin are food stage or pharmaceutical grade; Described modification chitin is carboxy methylation chitin.
Further preferably, above-mentioned soluble silver salt is silver nitrate or silver acetate.
Further preferably, above-mentioned reducing agent is natural ascorbic acid, vanillin or their mixture.
The technique effect that technique scheme is directly brought is, obtained trauma wound external application includes nanometer silver with functional toothpaste product, outward appearance is golden yellow gel, and it is applicable to the reparation in the hemostasis of skin injury, sterilizing, the antipruritic and skin injury face such as beriberi, eczema, knock damage, scratch, scald, burn.
The preparation method of technique scheme, for raw material with living marine resources sodium alginate and modification chitin, agree with reaction, silver ion complexation reaction, nanometer silver reaction by ion, add natural antibacterial moist reparation skin injury hardening agent and the anti-photosensitizer of nanometer silver, prepared can meet that Emergency use is convenient, film forming fast, hemostasis sterilizing is fast, there is bonding, the functional ointment of a kind of trauma wound external application of antipruritic, moist and reparation skin injury, that is a kind of liquid adhesive bandage product of silver colden visual appearance.Obtained product can be used as liquid bandage, and it has hemostasis, antipruritic, reparation skin, Promotive union, the effect of minimizing cicatrix; And there is the features such as film forming is fast, easy-clear, free from extraneous odour.Be applicable to the hemostasis of skin injury, sterilizing, and be applicable to that skin is antipruritic, the auxiliary treatment of beriberi or eczema, and the auxiliary treatment in the skin injury face such as scald, burn and reparation.
In sum, preparation method of the present invention, relative to prior art, has following beneficial effect:
1, by adopting carboxy methylation chitin, solve anionic polymer sodium alginate and can not prepare the technical barrier of water dispersible gel with cation high molecular modification chitin, do not agree with reaction by means of only macromole to have prepared simultaneously containing vegetalitas and animality ocean polysaccharide macromole, and the supermolecular gel of netted interpenetrating structure is the basic material that marine organisms nano-antibacterial gelation liquid adhesive bandage establishes bonding, film forming, macromole anion clotting mechanism; Because sodium alginate is only modification chitin price about 1/3rd, marine organisms nano-antibacterial gelation liquid adhesive bandage product is also made to reduce cost, marketing of being more convenient for.
2, nanometer silver has not only increased substantially the antibiotic property of gel, compensate for single sodium alginate and the weak defect of modification chitin antibiotic property, and plays crosslinked action, and network molecule is larger, and film property is better, and gel is more stable.
3, prepared product has two characteristic absorption peaks at 300nm place, ultra-violet (UV) band and visible ray 430nm place, and outward appearance is golden yellow gel, and silver gold paste becomes peculiar outward appearance mark and the item property of commodity.And the present invention with the addition of the silver-colored anti-light sensitive ingredients such as angelica keiskei koidzumi chalcone, can make silver gold paste in use and put procedure, black can not be become because of photosensitivity reaction, reduce or lose product usefulness.
4, rose polyphenol and Oleum Rosae Rugosae not only have nourishes rough skin, abnormal smells from the patient pleasant, promote wound healing effect, simultaneously, modification chitin mass-energy activating cell, promotion cell neogenesis are used, alginic acid chitin complex silver has hemostasis sterilization effect, its water absorbing properties keeps wound face to keep moist environment, supramolecular is more conducive to film forming, larger with the affinity of skin, difficult drop-off.Angelica keiskei koidzumi chalcone has sterilization, convergence, anti-silver-colored photosensitivity reaction, keeps the effect of product silver gold paste external appearance characteristic; Deep-sea dogfish alkene has nourishing, whitening, reparation skin, Promotive union, the effect of minimizing cicatrix.
So, the synergism of multifunctional dot, the like product cost performance that marine organisms nano-antibacterial gelation liquid adhesive bandage of the present invention is done than alginate or the chitosan of single component is higher, more can meet instant hemostasis, anti-infection, promoting healing, few cicatrix, ventilative aseptic wound face repair medical material requirement.
5, all material of the present invention is good biocompatibility, biodegradable natural material, not containing synthetic binding agent and volatile organic plasticiser, the adhesive plaster material not using difficult degradation, air-locked synthetic macromolecule membrane and easily cause allergic reaction, overcomes some defect of prior art.
Accompanying drawing explanation
The trauma wound external application of Fig. 1 obtained by embodiment 1 ultraviolet, the visible region characteristic absorption spectrum figure of functional toothpaste product;
Fig. 2 is the staphylococcus aureus inhibition zone test photo of the functional ointment embodiment of trauma wound external application;
The trauma wound external application of Fig. 3 obtained by embodiment 1 electromicroscopic photograph (amplification is 50,000 times) of functional ointment;
The trauma wound external application of Fig. 4 obtained by embodiment 10 electromicroscopic photograph (amplification is 50,000 times) of functional ointment.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described in detail.
Embodiment 1
Preparation method is as follows:
The first step, takes sodium alginate 5Kg and carboxy methylation chitin 50Kg respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution 500Kg that mass percent concentration is 1%; Carboxy methylation chitin matter is placed in container, and adding distil water is mixed with the modification chitin aqueous solution 500Kg that mass percent concentration is 10%;
Then, while stirring above-mentioned modification chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 60min, to make agreeing with and react completely therebetween, after concentrated, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 3 ︰ 1000, adds silver nitrate solution in hygroscopicity supermolecule GCP, stirs 30-60min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent ascorbic acid 296.5g, controlling reaction temperature is 10 DEG C, and under stirring condition, reaction 20min; After question response is complete, then add the reducing agent ascorbic acid of 0.1 mole, to make ascorbic acid excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
4th step, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 0.1% rose wate alcohol extract 1100.4g, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction 3310.2g and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion 1656g;
Golden yellow nanometer colloid base material is obtained after homogenizing; After golden yellow nanometer colloid base material packaging, product.
Embodiment 2
Replace to except silver acetate except by silver nitrate; All the other are all with embodiment 1.
Embodiment 3
Except by ascorbic acid 296.5g, replace to outside vanillin 256.1g; All the other are all with embodiment 1.
Embodiment 4
Except by ascorbic acid 296.5g, outside the mixture 276.3g replacing to ascorbic acid that 1:1 is in mass ratio mixed with and vanillin; All the other are all with embodiment 1.
Embodiment 5
Except the response time of agreeing with in the first step is replaced to except 90min by 60min; All the other are all with embodiment 1.
Embodiment 6
Except changing the 3rd step complex reaction temperature into 85 DEG C, the response time changes into outside 20min; All the other are all with embodiment 1.
Embodiment 7
Except the complex reaction temperature in the 3rd step being changed into 45 DEG C, the response time changes into except 40min; All the other are all with embodiment 1.
Embodiment 8
Preparation method is as follows:
The first step, takes sodium alginate 25Kg and carboxy methylation chitin 5Kg respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution 500Kg that mass percent concentration is 5%; Carboxy methylation chitin is placed in container, and adding distil water is mixed with the carboxy methylation chitin aqueous solution 500Kg that mass percent concentration is 1%;
Then, while stirring above-mentioned carboxy methylation chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 75min, to make agreeing with and react completely therebetween, after concentrated, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 2 ︰ 1000, adds silver nitrate solution in hygroscopicity supermolecule GCP, stirs 30-60min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent vanillin 93.1g, controlling reaction temperature is 25 DEG C, and under stirring condition, reaction 50min; After question response is complete, then add the reducing agent vanillin of 0.1 mole, to make vanillin excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
4th step, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 0.1% rose wate alcohol extract 600g, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction 1800g and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion 900g; Obtain golden yellow nanometer colloid base material after homogenizing, after packaging, product.
Embodiment 9
Preparation method is as follows:
The first step, takes sodium alginate 5Kg and carboxy methylation chitin 25Kg respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution 500Kg that mass percent concentration is 1%; Carboxy methylation chitin is placed in container, and adding distil water is mixed with the carboxy methylation chitin aqueous solution 500Kg that mass percent concentration is 5%;
Then, while stirring above-mentioned carboxy methylation chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 90min, to make agreeing with and react completely therebetween, after concentrated, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 1 ︰ 1000, adds silver nitrate solution in hygroscopicity supermolecule GCP, stirs 45min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent vanillin 46.6g, controlling reaction temperature is 50 DEG C, and under stirring condition, reaction 40min; After question response is complete, then add the reducing agent vanillin of 0.1 mole, to make vanillin excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
4th step, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 0.1% rose wate alcohol extract 600g, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction 1800g and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion 900g; Obtain golden yellow nanometer colloid base material after homogenizing, after packaging, product.
Embodiment 10
Preparation method is as follows:
The first step, takes sodium alginate 15Kg and carboxy methylation chitin 35Kg respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution 500Kg that mass percent concentration is 3%; Carboxy methylation chitin is placed in container, and adding distil water is mixed with the carboxy methylation chitin aqueous solution 500Kg that mass percent concentration is 7%;
Then, while stirring above-mentioned carboxy methylation chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 70min, to make agreeing with and react completely therebetween, after concentrated, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 1 ︰ 1000, adds the silver nitrate solution that mass percent concentration is 0.5% in hygroscopicity supermolecule GCP, stirs 35min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent ascorbic acid 448.9g, controlling reaction temperature is 60 DEG C, and under stirring condition, reaction 35min; After question response is complete, then add the reducing agent vanillin of 0.1 mole, to make vanillin excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
4th step, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 0.1% rose wate alcohol extract 1000g, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction 3000g and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion 1500g; Obtain golden yellow nanometer colloid base material after homogenizing, after packaging, product.
The inspection of product and detection:
The trauma wound external application chosen obtained by embodiment 1 carries out spectrum analysis with functional toothpaste product;
Choosing embodiment 1 and the functional toothpaste product of the trauma wound external application obtained by embodiment 10 respectively, to carry out the test of staphylococcus aureus inhibition zone and amplification be electron microscopy observation under 50,000 times.
Result is as follows:
The trauma wound external application of Fig. 1 obtained by embodiment 1 ultraviolet, the visible region characteristic absorption spectrum figure of functional toothpaste product;
As shown in Figure 1, product has maximum absorption band at 430nm place, visible region, and also has time high maximum absorption band at ultraviolet region 300nm place.This shows: obtained trauma wound external application argentiferous in functional toothpaste product, and outward appearance is golden yellow.
Fig. 2 is that embodiment 1 tests photo with the staphylococcus aureus inhibition zone of the respective obtained functional ointment of trauma wound external application of embodiment 10; In figure, numeral 1 is embodiment 1, numeral 2 is embodiment 10.
As shown in Figure 2: the trauma wound external application obtained by embodiment 1 is 20mm with the staphylococcus aureus inhibition zone radius of functional ointment; Trauma wound external application obtained by the embodiment 10 staphylococcus aureus inhibition zone radius 22mm of functional ointment.
Above-mentioned bacteriostatic experiment testing result shows: embodiment 1 and the respective obtained trauma wound external application of embodiment 10 all have significant antibacterial effect with functional ointment.
The trauma wound external application of Fig. 3 obtained by embodiment 1 electromicroscopic photograph of functional ointment, amplification is 50,000 times; The trauma wound external application of Fig. 4 obtained by embodiment 10 electromicroscopic photograph of functional ointment, amplification is 50,000 times.
As shown in Figure 3, Figure 4, from Fig. 3 and Fig. 4, all identical result can be seen: the mean diameter of nanometer silver is about 7nm.
This shows, the functional toothpaste product of trauma wound external application obtained by preparation method of the present invention, its nano effect is remarkable.
Product respectively obtained by Example 1-10 carries out antibacterial and haemostatic effect inspection, and result shows: this product 30min bacteriostasis rate all >=99.9%, the equal <10s of bleeding stopping period.
Claims (4)
1. a trauma wound external application preparation method for functional ointment, is characterized in that, comprise the following steps:
The first step is 1-5:1-10 in mass ratio, takes sodium alginate and modification chitin respectively, for subsequent use;
Sodium alginate is placed in container, and adding distil water is mixed with the sodium alginate aqueous solution that mass percent concentration is 1-5%; Modification chitin is placed in container, and adding distil water is mixed with the modification chitin aqueous solution that mass percent concentration is 1-10%;
Then, while stirring above-mentioned modification chitin aqueous solution is joined in above-mentioned sodium alginate aqueous solution; And Keep agitation 60-90min, to make agreeing with and react completely therebetween, obtain hygroscopicity supermolecule GCP;
Second step, by Ag
+ratio with the mass ratio of GCP is 1-5 ︰ 1000, adds soluble silver salt in hygroscopicity supermolecule GCP, stirs 30-60min, to make the two that complex reaction occur, obtains GCPAg
+supramolecular complex system;
3rd step, to GCPAg
+in supramolecular complex system, add molal quantity and Ag
+identical reducing agent, control reaction temperature is 10-85 DEG C, and under stirring condition, reaction 20-60min; After question response is complete, then add the reducing agent of 0.1 mole, to make this reducing agent excessive, obtain antioxidation supermolecule nano silver colloid GCPAg;
Above-mentioned reducing agent is natural ascorbic acid, vanillin or their mixture;
4th step, the ratio of 2%, 6% and 3% of antioxidation supermolecule nano silver colloid GCPAg quality is respectively in the quality of rose polyphenol, angelica keiskei koidzumi chalcone and squalene, in above-mentioned antioxidation supermolecule nano silver colloid GCPAg, to add effective ingredient be respectively the mass percent concentration of rose polyphenol be 1% rose wate alcohol extract, effective ingredient to be the mass percent concentration of angelica keiskei koidzumi chalcone be 30% angelica keiskei koidzumi chalcone water-alcohol extraction and effective ingredient to be the mass percent concentration of squalene be 10% squalene ethanol glycerol emulsion;
Golden yellow nanometer colloid base material is obtained after homogenizing; After golden yellow nanometer colloid base material packaging, product.
2. the trauma wound external application preparation method of functional ointment according to claim 1, is characterized in that, described sodium alginate and modification chitin are food stage or pharmaceutical grade; Described modification chitin is carboxy methylation chitin.
3. the trauma wound external application preparation method of functional ointment according to claim 1, is characterized in that, described soluble silver salt is silver nitrate or silver acetate.
4. the trauma wound external application preparation method of functional ointment according to claim 1, is characterized in that, described reducing agent is natural ascorbic acid, vanillin, or their mixture.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020054910A1 (en) * | 2000-08-08 | 2002-05-09 | Monteresearch S.R.L. | Topical composition for covering a skin lesion |
| CN1422671A (en) * | 2001-11-23 | 2003-06-11 | 吴天祥 | Medical paste film |
| CN103446621A (en) * | 2013-05-07 | 2013-12-18 | 哈尔滨工程大学 | Nano-silver-containing sodium alginate based antibacterial medical dressing and preparation method thereof |
| CN104257639A (en) * | 2014-10-15 | 2015-01-07 | 广东璞石医药科技有限公司 | Film coating agent for wound surface treatment and preparation method of film coating agent |
-
2016
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020054910A1 (en) * | 2000-08-08 | 2002-05-09 | Monteresearch S.R.L. | Topical composition for covering a skin lesion |
| CN1422671A (en) * | 2001-11-23 | 2003-06-11 | 吴天祥 | Medical paste film |
| CN103446621A (en) * | 2013-05-07 | 2013-12-18 | 哈尔滨工程大学 | Nano-silver-containing sodium alginate based antibacterial medical dressing and preparation method thereof |
| CN104257639A (en) * | 2014-10-15 | 2015-01-07 | 广东璞石医药科技有限公司 | Film coating agent for wound surface treatment and preparation method of film coating agent |
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