CN105475144B - Cherish Cornmarigold tissue culture medium (TCM) - Google Patents
Cherish Cornmarigold tissue culture medium (TCM) Download PDFInfo
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- CN105475144B CN105475144B CN201610106437.2A CN201610106437A CN105475144B CN 105475144 B CN105475144 B CN 105475144B CN 201610106437 A CN201610106437 A CN 201610106437A CN 105475144 B CN105475144 B CN 105475144B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
Cherish Cornmarigold tissue culture medium (TCM) the invention discloses one kind, bud inducement cultivation base is 50 g/l~70 g/l 6 benzyl aminoadenine of addition and 10 g/l~25 g/l heteroauxins using 2/3MS culture mediums as minimal medium；Root media is 15 g/l~30 g/l gibberellin of addition, 60 g/l~100 g/l glycine and 30 g/l~50 g/l peptones using MS culture mediums as minimal medium；Strong seedling culture base is 8 g/l~20 g/l methyl α-naphthyl acetates of addition, 5 g/l~10 g/l heteroauxins, 3 g/l~10 g/l Organic Seleniums and 10 g/l~20 g/l indolebutyric acids using 1/2MS culture mediums as minimal medium；Culture medium of the present invention can largely shorten the breeding cycle of bosom Cornmarigold, and gained seedling early growth is healthy and strong, and growing way is preferable.
Description
Technical field
The present invention relates to technical field of tissue culture, especially a kind of bosom Cornmarigold tissue culture medium (TCM).
Background technology
Bosom Cornmarigold is the Huai Chrysanthemi kind that Wen County Institute of agricultural sciences uses systematic breeding method to cultivate, perennial grass
This plant, stem band purple uprightly, grows thickly, high 85-110 centimetres, branch is less without rib, and whole body is close by white-colored hairs, base portion length wood
Matter, single leaf alternate, ovum shape lanceolar, blade celadon, leaf splits relatively deeply, and 4.9 centimetres of leaf length is wide 3.3 centimetres, 1.6 lis of handle length
Rice, capitulum, basidixed or axillary.Flower is in oblate spheroid, and 2.5 centimetres of diameter, inflorescence periphery ligulate flower number layer is arranged in many colyliforms,
It is rear in vain at the beginning of color and luster to turn pink, reddened through frost, centre is yellow tubular flower, mid or late October at florescence.Bosom Cornmarigold " color,
Shape " claims four absolutely, is used as medicine with flower bud or made tea, Long Drinks is clearing heat and detoxicating, nourishes the liver to improve visual acuity, sharp blood vessels；After bubbling open, delicate fragrance is assailed the nostrils, and is to occupy
Family, tourism, the treasure of present.Cherish Cornmarigold cold nature, micro-sweet, return lung, Liver Channel, purine-containing, choline, little Su alkali, amino acid etc.
It is a variety of to human body beneficiating ingredient.
At present, bosom Cornmarigold seedling is bred mainly by traditional offshoot, causes reproduction speed slow, and bosom Cornmarigold length
Gesture is bad, and axillary seedling is irregular, and tissue cultures can realize the quick breeding of bosom Cornmarigold, and the domestic research to cherishing Cornmarigold
It is less.
The content of the invention
Cherish Cornmarigold tissue culture medium (TCM) it is an object of the invention to provide one kind, this bosom Cornmarigold tissue culture medium (TCM) can be carried
For a kind of new propagation method.
In order to solve the above problems, the technical solution adopted by the present invention is：
This bosom Cornmarigold tissue culture medium (TCM), including bud inducement cultivation base, root media and strong seedling culture base：
Bud inducement cultivation base is, using 2/3MS culture mediums as minimal medium, to add 50 g/l~70 g/l 6- benzyl amino
Adenine and 10 g/l~25 g/l heteroauxins；
Root media is 15 g/l~30 g/l gibberellin of addition, 60 g/l using MS culture mediums as minimal medium
~100 g/l of glycine and 30 g/l~50 g/l peptones；
Strong seedling culture base be using 1/2MS culture mediums as minimal medium, 8 g/l~20 g/l methyl α-naphthyl acetates of addition, 5 grams/
Rise~10 g/l of heteroauxins, 3 g/l~10 g/l Organic Seleniums and 10 g/l~20 g/l indolebutyric acids.
In above-mentioned technical proposal, more specifically technical scheme can also be：Organic Selenium is seleno egg glycine or selenium
For cystamine hydrochloride.
MS medium components are：1.9 g/l of potassium nitrate, 1.65 g/l of ammonium nitrate, the mg/litre of potassium dihydrogen phosphate 170, sulphur
The sour mg/litre of magnesium 370, the mg/litre of calcium chloride 440, the mg/litre of KI 0.83, the mg/litre of boric acid 6.2, the milli of manganese sulfate 22.3
G/l, the mg/litre of zinc sulfate 8.6, the mg/litre of sodium molybdate 0.25, the mg/litre of copper sulphate 0.25,0.025 milligram of cobalt chloride/
Liter, the mg/litre of disodium ethylene diamine tetraacetate 37.25, the mg/litre of ferrous sulfate 27.85, the mg/litre of inositol 100, the milli of glycine 2
G/l, the mg/litre of thiamine hydrochloride 0.1, the mg/litre of puridoxine hydrochloride 0.5, the mg/litre of nicotinic acid 0.5, the mg/litre of sucrose 30,
The mg/litre of agar 7.
By adopting the above-described technical solution, the present invention has the advantages that compared with prior art：
Culture medium of the present invention can largely shorten the breeding cycle of bosom Cornmarigold, and gained seedling early growth is healthy and strong, growing way
Preferably, inhereditary feature is more consistent, is that the breeding for cherishing Cornmarigold is not limited by season, can be widely applied.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
The present embodiment cherishes Cornmarigold tissue culture medium (TCM), including bud inducement cultivation base, root media and strong seedling culture base：
Bud inducement cultivation base is, using 2/3MS culture mediums as minimal medium, to add 50 g/l of 6- benzyls aminoadenines and 25
G/l heteroauxin.
Root media be using MS culture mediums as minimal medium, addition 15 g/l of gibberellin, 80 g/l of glycine and
30 g/l of peptones.
Strong seedling culture base is, using 1/2MS culture mediums as minimal medium, to add 20 g/l of methyl α-naphthyl acetates, 10 g/l of indoles second
Acid, 10 g/l of seleno egg glycines and 20 g/l of indolebutyric acids.
MS medium components are：1.9 g/l of potassium nitrate, 1.65 g/l of ammonium nitrate, the mg/litre of potassium dihydrogen phosphate 170, sulphur
The sour mg/litre of magnesium 370, the mg/litre of calcium chloride 440, the mg/litre of KI 0.83, the mg/litre of boric acid 6.2, the milli of manganese sulfate 22.3
G/l, the mg/litre of zinc sulfate 8.6, the mg/litre of sodium molybdate 0.25, the mg/litre of copper sulphate 0.25,0.025 milligram of cobalt chloride/
Liter, the mg/litre of disodium ethylene diamine tetraacetate 37.25, the mg/litre of ferrous sulfate 27.85, the mg/litre of inositol 100, the milli of glycine 2
G/l, the mg/litre of thiamine hydrochloride 0.1, the mg/litre of puridoxine hydrochloride 0.5, the mg/litre of nicotinic acid 0.5, the mg/litre of sucrose 30,
The mg/litre of agar 7.
Embodiment 2
The present embodiment cherishes Cornmarigold tissue culture medium (TCM), including bud inducement cultivation base, root media and strong seedling culture base：
Bud inducement cultivation base is, using 2/3MS culture mediums as minimal medium, to add 60 g/l of 6- benzyls aminoadenines and 20
G/l heteroauxin.
Root media be using MS culture mediums as minimal medium, addition 20 g/l of gibberellin, 100 g/l of glycine and
40 g/l of peptones.
Strong seedling culture base is, using 1/2MS culture mediums as minimal medium, to add 8 g/l of methyl α-naphthyl acetates, 5 g/l of indoles second
Acid, 3 g/l of selenocystamine hydrochlorides and 10 g/l of indolebutyric acids.
MS medium components are：1.9 g/l of potassium nitrate, 1.65 g/l of ammonium nitrate, the mg/litre of potassium dihydrogen phosphate 170, sulphur
The sour mg/litre of magnesium 370, the mg/litre of calcium chloride 440, the mg/litre of KI 0.83, the mg/litre of boric acid 6.2, the milli of manganese sulfate 22.3
G/l, the mg/litre of zinc sulfate 8.6, the mg/litre of sodium molybdate 0.25, the mg/litre of copper sulphate 0.25,0.025 milligram of cobalt chloride/
Liter, the mg/litre of disodium ethylene diamine tetraacetate 37.25, the mg/litre of ferrous sulfate 27.85, the mg/litre of inositol 100, the milli of glycine 2
G/l, the mg/litre of thiamine hydrochloride 0.1, the mg/litre of puridoxine hydrochloride 0.5, the mg/litre of nicotinic acid 0.5, the mg/litre of sucrose 30,
The mg/litre of agar 7.
Embodiment 3
The present embodiment cherishes Cornmarigold tissue culture medium (TCM), including bud inducement cultivation base, root media and strong seedling culture base：
Bud inducement cultivation base is, using 2/3MS culture mediums as minimal medium, to add 70 g/l of 6- benzyls aminoadenines and 10
G/l heteroauxin.
Root media is 30 g/l of gibberellin of addition, 60 g/l and 50 g/l using MS culture mediums as minimal medium
Peptone.
Strong seedling culture base is, using 1/2MS culture mediums as minimal medium, to add 15 g/l of methyl α-naphthyl acetates, 7.5 g/l of indoles
Acetic acid, 7 g/l of selenocystamine hydrochlorides and 15 g/l of indolebutyric acids.
MS medium components are：1.9 g/l of potassium nitrate, 1.65 g/l of ammonium nitrate, the mg/litre of potassium dihydrogen phosphate 170, sulphur
The sour mg/litre of magnesium 370, the mg/litre of calcium chloride 440, the mg/litre of KI 0.83, the mg/litre of boric acid 6.2, the milli of manganese sulfate 22.3
G/l, the mg/litre of zinc sulfate 8.6, the mg/litre of sodium molybdate 0.25, the mg/litre of copper sulphate 0.25,0.025 milligram of cobalt chloride/
Liter, the mg/litre of disodium ethylene diamine tetraacetate 37.25, the mg/litre of ferrous sulfate 27.85, the mg/litre of inositol 100, the milli of glycine 2
G/l, the mg/litre of thiamine hydrochloride 0.1, the mg/litre of puridoxine hydrochloride 0.5, the mg/litre of nicotinic acid 0.5, the mg/litre of sucrose 30,
The mg/litre of agar 7.
Claims (1)
1. one kind bosom Cornmarigold tissue culture medium (TCM), including bud inducement cultivation base, root media and strong seedling culture base, its feature exist
In：
Bud inducement cultivation base is that, using 2/3MS culture mediums as minimal medium, 50 g/l~70 g/l 6- benzyl amino glands of addition are fast
Purine and 10 g/l~25 g/l heteroauxins；
Root media be using MS culture mediums as minimal medium, 15 g/l~30 g/l gibberellin of addition, 60 g/l~
100 g/l of glycine and 30 g/l~50 g/l peptones；
Strong seedling culture base be using 1/2MS culture mediums as minimal medium, 8 g/l~20 g/l methyl α-naphthyl acetates of addition, 5 g/l~
10 g/l of heteroauxins, 3 g/l~10 g/l Organic Seleniums and 10 g/l~20 g/l indolebutyric acids；The Organic Selenium is
Seleno egg glycine or selenocystamine hydrochloride.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610106437.2A CN105475144B (en) | 2016-02-26 | 2016-02-26 | Cherish Cornmarigold tissue culture medium (TCM) |
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| CN201610106437.2A CN105475144B (en) | 2016-02-26 | 2016-02-26 | Cherish Cornmarigold tissue culture medium (TCM) |
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| CN105475144B true CN105475144B (en) | 2017-09-29 |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1076161C (en) * | 1997-10-10 | 2001-12-19 | 中国科学院昆明植物研究所 | Technology for germ plasma preservation and quick breeding by group culture of pyrethrum |
| CA2267012A1 (en) * | 1999-03-25 | 2000-09-25 | University Of Guelph | Micropropagation of phytopharmaceutical plants |
| CN102342247A (en) * | 2011-09-28 | 2012-02-08 | 金色种业有限公司 | Selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium |
| RU2479983C1 (en) * | 2011-10-27 | 2013-04-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Горский государственный аграрный университет" | Method of increasing net reproduction of meristematic potato tubers |
| CN103975851B (en) * | 2014-03-31 | 2016-01-27 | 中国计量学院 | A kind of method of FLOS CHRYSANTHEMI ALBA from Haizhou of China tissue cultures and breeding |
| CN104094845B (en) * | 2014-06-26 | 2016-08-24 | 三峡大学 | A kind of in-vitro culture method of Dendranthema indicum |
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