CN105455855B - Lactic acid measuring equipment and the method for training adjustment - Google Patents
Lactic acid measuring equipment and the method for training adjustment Download PDFInfo
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- CN105455855B CN105455855B CN201410448944.5A CN201410448944A CN105455855B CN 105455855 B CN105455855 B CN 105455855B CN 201410448944 A CN201410448944 A CN 201410448944A CN 105455855 B CN105455855 B CN 105455855B
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Abstract
A kind of method that the present invention discloses lactic acid measuring equipment and training adjustment, the method for training adjustment measure lactic acid concn in human body by the lactic acid measuring equipment comprising a continuous percutaneous micropin sensor, and the method for this training adjustment comprises the following steps：The lactic acid concn value in user's tissue is measured using the continuous percutaneous micropin sensor；Compare lactic acid concn value and a benchmark preset value；And if lactic acid concn value higher than benchmark preset value then notify user reduce exercise intensity, if otherwise lactic acid concn value less than benchmark preset value then notify user increase exercise intensity.The present invention also provides a kind of lactic acid measuring equipment.
Description
Technical field
It is more particularly to a kind of to measure people by percutaneous micropin sensor the present invention relates to a kind of method of training adjustment
The method that lactic acid concn adjusts for training in body.
Background technology
Carbohydrate is mainly used in movement, and as energy source, carbohydrate generates energy and is divided into aerobic and anaerobic two ways, candy
Class is decomposed under anaerobic situation, in addition to it can generate energy, can also generate lactic acid.When exercise intensity is relatively low, main use has
The mode of oxygen generates energy, and anaerobic participation is less, therefore the speed that lactic acid generates is not high, and human body can be easy to metabolism and fall, institute
It will not be accumulated in body and blood with lactic acid.With the increase of exercise intensity, no oxygen energy usage rate improves, and lactic acid generates
Speed also speed therewith, the speed that human body removes lactic acid can gradually be unable to catch up with the speed of lactic acid generation, therefore lactic acid will be opened
Beginning slowly accumulates in the body, and under this exercise intensity, the Lactate in human body is referred to as lactic acid threshold value (Lactate
Threshold).On the time point of lactic acid threshold value between aerobic metabolism and anaerobic metabolism, the lactate level in human body can be kept
Balance.If the lactic acid threshold value of sportsman and relevant cardiac rate are it is known that can be according to data above optimization to the movement
The training of member.
Lactic acid is tissue oxygenation (oxygenation) most important biomarker, therefore to physical culture, the application of health care
It is particularly important.Lactic acid concn provides the information of anaerobic threshold, this information is very important to working out endurance exercise training program.Mesh
The commercial interest driving of preceding lactic acid measuring equipment comes from movement, body-building, dairy produce and national defense industry.
The normal blood lactate concentration of static rest reaches anaerobic threshold in the range of 0.5-2mM in strenuous exercise
Or in the case of injured initiation haemorrhagic shock, when cell oxygen-supplying amount becomes limited, lactic acid can increase sharply.Measurement lactic acid helps
In identification fatigue reaction (to sportsman, other physical culture personage and soldier are even more important) and the personalized trained flow of design to fortune
It mobilizes.Lactic acid also to many Intensive Care Therapies in the case of have clinical meaning, for example, lactic acidosis, especially embodies
In shock state.
Have physiology of exercise scholar think movement in and movement after blood lactase acid variation, be in the tissues such as skeletal muscle lactic acid generate
Rate, the performance balanced into the rate of blood and blood between disappearance of lactic acid rate.Therefore, exercise intensity, duration,
Metabolism between each histoorgan, it is all related with the concentration of blood lactase acid, during exercise with after movement, can continuously detect blood breast
The influence of human body and its rule when sour Come understands movement.Utilization of the test of blood lactase acid on movement instruction Practice, instructing the content of Practice is
One considerably complicated work is planned to provide a more perfect instruction Practice course and instruction Practice, and religion Practice usually requires to find out
One can be used as in blood level, rest value about 1mM to the optimal amount of exercise of sportsman and stimulation, lactic acid and judge movement
The foundation of intensity when lactic acid concn reaches 4.0mM in blood, can be used as and judge anaerobic threshold.Prior document is pointed out, does dynamic fierceness
During movement, lactic acid concn has up to 30 μM/g in blood.Separately there is document to point out, when intramuscular lactic acid concn reaches 20-25 μM/g
When, it makes one meeting fatigue completely and can not move.
There is the principal element that document points out that lactic acid generates in movement to be again：First, the load intensity of movement；2nd, Ginseng and movement
Muscle mass；3rd, the time of motion continuation.Lactic acid generate concentration number, then with (aerobic) aerobic in motion process and nothing
The percentage of oxygen (anaerobic) system capacity metabolism is related.Different energy system outputs, blood lactase acid during for human motion
Value influences the variation of its concentration.Blood lactate concentration is less when energy source is supplied based on phosphoric acid system, does not in general surpass
Cross 4mM；If during using glycolysis system as main supplying energy source, 15mM can be up to；Using aerobic energy system as mainly for
In 4mM when should be able to measure.Therefore after the training of varying strength, the accumulation situation of blood lactase acid has significant otherness.Lactic acid is accumulated
For tool there are two types of meaning, a kind of is because accelerating the effect of glycolysis, generates substantial amounts of energy so that contraction of muscle uses；It is another to be
Represent that the degree being acidified in muscle improves, original aerobic metabolism system function is not applied and used, and bad anaerobic glycolysis effect entirely comes
Energy is provided.In addition, there is physiology of exercise scholar to think in the case where stablizing Like states, the situation of blood lactase acid accumulation, which can be used as, judges movement
One of biological parameter of load.
Lactic acid is accumulated most common the reason is that anoxic (hypoxia) (low tissue and blood oxygenation).It is measured outside laboratory
The standard method of lactic acid concn is discrete blood sampling and measures through lactic acid test paper.However, this is to dynamic change or pre-
Measurement information helps less, and may cause to lack an accurate concentration reestablishing.Discrete sampling is actually not convenient for endurance work
It is carried out in the case of dynamic.In addition, it can not directly lay down a definition to result.
The lactic acid measuring equipment for being implanted directly into blood vessel is not a practical selection, the reason is that thrombus and embolism can be caused
Risk triggers through the interaction between blood/lactic acid measuring equipment, and allows and generate dynamics change by local blood flow
Change.The measurement of percutaneous tissue liquid provides a safer option, and any adverse effect can localize.And the lactic acid in organizing
Measurement also can provide more relevant informations, so as to detect local tissue hypoxia rather than pass through to local oxygen supply
Blood analysis obtains the average value of body.
Lactate is measured using lactic acid measuring equipment, is when sportsman is in different motion intensity, blood sampling is analyzed.Medical care
The method of personnel's sampling blood adopts and has an acupuncture treatment and wear out cuticula mostly, to extract blood to carry out analysis detection, however it is such broken
The sampling method of bad skin surface except easily making sportsman's feels pain, and then germinates outside rejection feeling, skin surface it is a large amount of
Microorganism, it is also easily destroyed in skin surface, into human body and then infection.In order to reduce the risk of infection, need
High sanitary standard is wanted, thus the method is made to become complicated and expensive.
In addition, the method that general non-intrusion type measures lactic acid is gradually to be in fashion to test using the Kang Keni in sports medical science
(Conconi test).In the method, on 400 meters of athletic tracks, first 200 meters are run testee with a predetermined speed
Step, 200 meters of testee's segmentations afterwards increase rhythm, such as 0.5km/h respectively.In the every one 200 meters of marks of athletic tracks,
Testee pays attention to his cardiac rate at that time, and barks out data to assistant after circumduction runway respectively.Testee is moving
It runs on runway, until he has arrived at strength limits, it is meant that he can not be further added by speed.
For the estimation of test, cardiac rate is mapped against relevant speed of running with two-dimentional (X-Y).Thereby find result：
Aerobic scope, provides relatively low strength, and cardiac rate is almost in a linear relationship with race speed.This means that cardiac rate is produced with testee
Raw strength equal proportion increases.This rule is broken at threshold value of the conversion to anaerobic metabolism.In the anaerobic scope of high output, the heart
Jump rate only slightly increases with further increased strength or race speed.Cardiac rate be relevant to run speed relation thus show from it is low go out
The aerobic scope of power transits to clear, the sharp discontinuity of the anaerobic scope of high output, thereby determines lactic acid threshold value.For breast
Sour threshold value and the related cardiac rate feature for running speed simply can be schemed to read from X-Y.
However, Kang Keni test and comparisons are complicated, and can not almost be performed in the case of not having assistant.Also, due to weather and
Testee controls the capacity of his race speed, and the lactic acid threshold value comparison that Kang Keni tests measure is inaccurate.
In summary, it is necessary to which a kind of low invasive lactic acid measuring equipment available in continuous percutaneous carbon dioside monitoring tissue, provides
To movement, body-building and national defense industry are used, particularly sportsman and movement hobby personage, are made it that muscle be kept not ache, and are adjusted
Its whole exercise intensity reaches the fitness goals not injured effectively.
The content of the invention
The purpose of the present invention is to provide a kind of method of training adjustment, by the percutaneous sensing with micropin
Device carries out skin penetrating, and low invasive puncture can effectively mitigate the feeling of pain of user, and reach sampling tissue fluid simultaneously
To measure the purpose of lactic acid concn value in human body.According to the comparison of lactic acid concn value and a benchmark preset value, help sportsman or
The personage that is keen on sports adjusts exercise intensity and frequency, and then the training of most effective fruit.
In order to reach above-mentioned purpose, the present invention provides a kind of method of training adjustment, is continuously passed through by including one
The lactic acid measuring equipment of skin micropin sensor measures lactic acid concn in human body, and the method for this training adjustment includes following step
Suddenly：The lactic acid concn value in user's tissue is measured using continuous percutaneous micropin sensor；Compare lactic acid concn value and one
Benchmark preset value；And if lactic acid concn value higher than benchmark preset value then notify user reduce exercise intensity, if otherwise breast
Acid concentration value then notifies user to increase exercise intensity less than benchmark preset value, it is ensured that user's muscle is not ached, it is sufficient to complete complete
Journey is competed.The distance further completed according to estimations such as GPS or pedometers and the distance for waiting to complete, can effectively adjust
The lactic acid concn value and its safe change rate of concentration of safety, to improve the achievement of athlete's.Likewise, for various
Ball match or the match of various team sports, personal physical ability situation, if it needs to rest or sustainable completion, lactic acid concn value
All being with its change rate of concentration can be as the foundation effectively referred to.
It is another object of the present invention to provide a kind of lactic acid measuring equipment, by the percutaneous sensor with micropin into
Row skin penetrating, low invasive puncture can effectively mitigate the feeling of pain of user, and reach sampling tissue fluid simultaneously to measure
Survey the purpose of lactic acid concn value in human body.
In order to reach above-mentioned purpose, the present invention also provides for a kind of lactic acid measuring equipment, lactic acid measuring equipment includes one
Continuous percutaneous micropin sensor and a comparator, comparator are used to compare the signal processing list from continuous percutaneous micropin sensor
The lactic acid measuring value and a benchmark preset value of member.The running of the lactic acid measuring equipment of the present invention is based on lactic acid sensing ferment generation
Electroactive hydrogen peroxide, and then the platinum electrode generation Ampere currents that polarizes at one, selectivity and group are realized dependent on interior outer membrane
Knit biocompatibility.The lactic acid measuring equipment of the present invention can be fully calibrated, from chaff interferent (such as ascorbic acid common in vivo
And paracetamol) influence.It is wide with the range of linearity, entire physiological range (up to 25mM) is covered, with the height to lactic acid
Degree selectivity.
Blood lactase acid accumulation initial value (the onset of blood lactate accumulation, OBLA) is corresponding
In the exercise intensity of 4mM lactic acid concns, it represents the maximum working load of lactic acid concn under stable state, and corresponding to from allowing
Load is transitioned into more serious exercise intensity.Different researchs show importance of the OBLA in long-distance running performance, it is also considered as
Sensitive identification beacon between the sensitive indicator adapted to caused by training and elite and outstanding sportsman.
The method of training or the match adjustment of the present invention, adjusts the strategy of exercise intensity substantially with lactic acid threshold value
(lactate threshold, LT) or anaerobic threshold, maximum lactic acid steady-state value (maximal lactate steady state,
), MLSS or blood lactase acid accumulates initial value (the onset of blood lactate accumulation, OBLA) conduct
Benchmark preset value, when lactic acid measuring value be more than this benchmark preset value, and lactic acid changing value increase, then exercise intensity can drop
It is low.To observe whether lactic acid changing value is reduced, until lactic acid measuring value returns to benchmark preset value.If being more than benchmark preset value,
But still exercise intensity is maintained, and and lactic acid changing value is close to zero, then it represents that, the physical ability situation of sportsman today is good, Ke Yiwei
Hold exercise intensity at this time.Further, sportsman can slightly increase exercise intensity, and observe whether lactic acid measuring value persistently increases
Add, still increase to an equalization point, that is, remain unchanged.By so effective training, sportsman in identical lactic acid preset value,
Previous exercise intensity can be broken through, such as is run, then its speed can be accelerated；Such as swim, then its swimming rate can be accelerated, because
This games results can all improve.
Another embodiment is for one with personal anaerobic threshold (individual anaerobic threshold, IAT)
Preset reference value, IAT are defined as having a net increase of long 1.5mM's than lactic acid threshold value (lactate threshold, LT) in lactic acid concn
When velocity.
Continuous percutaneous micropin sensor according to the present invention, this micropin sensor include：Substrate, microneedle unit, at signal
Manage unit and power supply unit.Microneedle unit includes at least the first micropin group being arranged on substrate as working electrode, Yi Jizuo
For the second micropin group of reference electrode, each micropin group includes at least a micropin, and the first micropin group includes an at least thin slice, each
One perforation is at least set on thin slice, and perforated edge is provided with a bur, wherein the perforation on a thin slice is on remaining thin slice
The bur of the perforated edge of relative position passes through, and those burs are disconnected from each other.
Compared to the prior art, the present invention measures the breast in user's tissue using a continuous percutaneous micropin sensor
Acid concentration value, low invasive puncture can reduce the risk of infection.Moreover, micropin sensor used in the method for the present invention can
Lactic acid concn value in simplicity wearing, accurate measurement tissue is conducive to user and more accurately knows the physiology ginseng of oneself
Number, help adjust exercise intensity and frequency, and then the training of most effective fruit.
Below in conjunction with the drawings and specific embodiments, the present invention will be described in detail, but not as a limitation of the invention.
Description of the drawings
The step flow chart of the method for Fig. 1 one embodiment of the invention training adjustment.
The explosive decomposition figure of the continuous percutaneous micropin sensor of Fig. 2 one embodiment of the invention；
The explosive decomposition figure of the continuous percutaneous micropin sensor of one embodiment of the invention of Fig. 3 and Fig. 2 difference view directions；
The schematic diagram of Fig. 4 one embodiment of the invention microneedle units；
The structure partial top view of Fig. 5 one embodiment of the invention working electrode micropin groups；
The structure partial top view of Fig. 6 another embodiment of the present invention working electrode micropin groups；
The structure partial top view of Fig. 7 another embodiment of the present invention working electrode micropin groups；
The structure partial top view of the still another embodiment working electrode micropin group of Fig. 8 present invention；
The combination schematic appearance of the continuous percutaneous micropin sensor of Fig. 9 one embodiment of the invention；
The combination schematic cross-sectional view of the continuous percutaneous micropin sensor of Figure 10 one embodiment of the invention；
Figure 11 is the partial schematic sectional view of Figure 11, and wherein lactic acid sensing ferment is coated on the bur of micropin；
Figure 12 is the close-up schematic view of bur in Figure 11；
Figure 13 is the partial schematic sectional view of Figure 10, and wherein lactic acid sensing ferment is coated on test-paper；And
Figure 14 is the close-up schematic view of test piece paper in Figure 13.
Specific embodiment
Detailed description for the present invention and technology contents, cooperation description of the drawings is as follows, however appended attached drawing only provides
With reference to illustrating to use, not it is used for being any limitation as the present invention.
It refer to Fig. 1, the method for Fig. 1 one embodiment of the invention training adjustment, by continuous percutaneous micro- comprising one
The lactic acid measuring equipment of pin sensor measures lactic acid concn in human body, and the method for this training adjustment comprises the following steps：Step
Rapid S10 measures the lactic acid concn value in user's tissue using a continuous percutaneous micropin sensor；Step S12, it is more newborn
Acid concentration value and a benchmark preset value；And step S14, if lactic acid concn value then notifies user to reduce higher than benchmark preset value
Exercise intensity, if otherwise lactic acid concn value then notifies user to increase exercise intensity less than benchmark preset value.In step S12
Benchmark preset value is the basic physiological data with reference to common people, such as age, gender, height and weight etc., the lactic acid of acquisition are dense
Angle value.
In one embodiment of the invention, lactic acid measuring equipment includes a continuous percutaneous micropin sensor and a comparator (figure
Do not show), comparator is for comparing the lactic acid measuring value and a benchmark preset value from the signal processing unit.
Fig. 2 and Fig. 3 are refer to, Fig. 2 and Fig. 3 watch one embodiment of the invention continuously percutaneous micropin by different directions respectively
The explosive decomposition figure of sensor.The continuous percutaneous micropin sensor of the present invention includes：Substrate 10, microneedle unit 20, flexible pad
Piece 30, signal processing unit 41, power supply unit 43 and outer cover 50, wherein signal processing unit 41 and power supply unit 43 are disposed on
On circuit board 40.
An embodiment according to the present invention, microneedle unit 20, which includes to be arranged on substrate 10, covers sulfonated polyether ether sulfone/poly-
Ether sulfone film and lactic acid sensing ferment as working electrode the first micropin group 22, cover yttrium oxide or the compatible conduction of other biological
Material is as the 3rd micropin group 26 of the second micropin group 24 with reference to electrode and covering platinum as counterelectrode.First micropin
Those micropins of group 22 may, for example, be array format and be arranged on substrate 10.There is an opening 32 for micro- on flexible gasket 30
Pin unit 20 is by the way that and microneedle unit 20 is electrically connected with conductive column 21,23,25 and the electric contact 42,44,46 on circuit board 40
It connects.Since the present invention has a flexible gasket 30, when operation, can be in close contact with the muscle profile syntype of user.Work as micropin
Group is inserted into subcutaneous tissue, and applies polarizing voltage between electrode, and lactic acid sensing ferment is generated with the lactic acid reaction in tissue fluid
Electroactive hydrogen peroxide (H2O2), and then generate Ampere currents, electric current relation proportional to lactic acid concn in working electrode.
Signal processing unit 41 is electrically connected to receive the concentration of target molecules of micropin sensing with microneedle unit 20, through computing
After judgement, a sensing signal and a kind of signal that can reflect the physiological status of user instantly are converted the information into.Power supply
Unit 43 is to supply power for operation to the continuous percutaneous micropin sensor of the present invention.
It refer to Fig. 4, the schematic diagram of Fig. 4 one embodiment of the invention microneedle units.First micropin group, 22 system is thin by first
222 and second thin slice 224 of piece is stacked to be formed, and one first perforation 2222 is at least set on the first thin slice 222, first perforation 2222
Edge, which is provided on one first bur 2224 and the second thin slice 224, at least sets one second perforation 2242, and the second perforated edge is set
One second bur 2244 is equipped with, the second bur 2244 passes through the first perforation 2222 and first of relative position on the first thin slice 222
Bur 2224 is opposite.In addition, barb 2246 and the hole on substrate 10 can be set on 224 edge of the second thin slice of first micropin group 22
Cave 102 engages.It can be set in an embodiment, 224 edge of the second thin slice of the first micropin group 22 and lead the insertion substrate 10 of handle 2248
On slot 104, be electrically connected by circuit and conductive column 21.
Similarly, the second micropin group 24 also has the first thin slice 242, and one first perforation is at least set on the first thin slice 242
2422, the first perforated edge is provided with one first bur 2424.It in addition, can on 242 edge of the first thin slice of the second micropin group 24
Barb 2426 is set to engage with the hole 102 on substrate 10.At an embodiment, 242 edge of the first thin slice of the second micropin group 24
On can set lead handle 2428 be inserted into substrate 10 on slot 104, be electrically connected by circuit and conductive column 23.
Similarly, the 3rd micropin group 26 also has the first thin slice 262, and one first perforation is at least set on the first thin slice 262
2622, the first 2622 edges of perforation are provided with one first bur 2624.In addition, 262 edge of the first thin slice of the 3rd micropin group 26
On barb 2626 can be set to engage with the hole 102 on substrate 10.In an embodiment, the first thin slice 262 of the 3rd micropin group 26
It can be set on edge and lead the slot 104 that handle 2628 is inserted on substrate 10, be electrically connected by circuit and conductive column 25.
One embodiment of the invention, the micropin of the first micropin group 22, the second micropin group 24 and the 3rd micropin group 26 be by
Punching press or etch process are formed.The material of those burs be selected from stainless steel, nickel, nickel alloy, titanium, titanium alloy, carbon nanotubes or
Silicon materials.The material of those burs can also be that resin is, for example, makrolon, polymethacrylic acid copolymer, ethylene/acetic acid
Vinyl ester copolymers, Teflon or polyesters, and in metal of the surface deposition with biocompatibility.The height of those burs is
300-600 microns, base widths be 150-450 microns.The point of those burs at intervals of 500-3000 microns.
Fig. 5 be refer to Fig. 8.The structure partial top view of Fig. 5 one embodiment of the invention working electrode micropin groups.First
Micropin group 22 is formed by the first thin slice 222 and the second thin slice 224 are stacked, and one first perforation is at least set on the first thin slice 222
2222, the first 2222 edges of perforation, which are provided on one first bur 2224 and the second thin slice 224, at least sets one second perforation
2242, the second perforated edge is provided with one second bur 2244, and the second bur 2244 passes through relative position on the first thin slice 222
First perforation 2222 and the first bur 2224 are opposite.
The structure partial top view of Fig. 6 another embodiment of the present invention working electrode micropin groups.First micropin group 22 be by
First thin slice 222, the second thin slice 224 and the 3rd thin slice 226 are stacked to be formed, and the first perforation is at least set on the first thin slice 222
2222, the first 2222 edges of perforation are provided with one first bur 2224, and one second perforation is at least set on the second thin slice 224
2242, the second 2242 edges of perforation, which are provided on one second bur 2244 and the 3rd thin slice 226, at least sets one the 3rd perforation
2262, the 3rd 2262 edges of perforation are provided with one the 3rd bur 2264, and the second bur 2244 and the 3rd bur 2264 pass through first
The first perforation 2222 and the first bur 2224 on thin slice 222 are in positive triangle taper.
The structure partial top view of Fig. 7 another embodiment of the present invention working electrode micropin groups.First micropin group 22 be by
First thin slice 222, the second thin slice 224, the 3rd thin slice 226 are stacked to be formed, wherein at least setting one first to wear on the first thin slice 222
Hole 2222, the first 2222 edges of perforation are provided with one first bur 2224；One second perforation is at least set on the second thin slice 224
2242, the second 2242 edges of perforation are provided with one second bur 2244；And the 3rd at least set one the 3rd perforation on thin slice 226
2262, the 3rd 2262 edges of perforation are provided with one the 3rd bur 2264, by the second bur 2244 and the 3rd bur 2264 through the
The first perforation 2222 and 2224 arranged adjacent of the first bur on one thin slice 222, in isosceles right angle trigonometry taper.
The structure partial top view of the still another embodiment working electrode micropin group of Fig. 8 present invention.First micropin group 22 is
It is formed by the first thin slice 222, the second thin slice 224, the 3rd thin slice 226 and the 4th thin slice 228 are stacked, on the first thin slice 222 at least
One first perforation 2222 is set, and the first 2222 edges of perforation are provided with one first bur 2224, are at least set on the second thin slice 224
One second perforation 2242, the second 2242 edges of perforation are provided with one second bur 2244, one the are at least set on the 3rd thin slice 226
Three perforation 2262, the 3rd 2262 edges of perforation, which are provided on one the 3rd bur 2264 and the 4th thin slice 228, at least sets one the 4th
Perforation 2282, the 4th 2282 edges of perforation are provided with one the 4th bur 2284, the second bur 2244, the 3rd bur 2264 and the 4th
Bur 2284 is through the first perforation 2222 on the first thin slice 222 and the first bur 2224 in corner taper.
For Fig. 5 into four embodiments shown in Fig. 8, each bur 2224 of the first micropin group 22 includes a point 2221
And a substrate 2223, wherein the shape after the bur of the perforated edge of relative position on remaining thin slice passes through of the perforation on a thin slice
Into the micropin those points top not in sustained height.Alternatively, the order that can be overlapped according to those thin slices, in advance
The height of its bur is designed, wears bur of the perforation through the perforated edge of relative position on remaining thin slice on a wherein thin slice
The top of those points of the micropin formed later has sustained height.
Then, Fig. 9 and Figure 10 be refer to.The combination appearance of the continuous percutaneous micropin sensor of Fig. 9 one embodiment of the invention
Schematic diagram.The combination schematic cross-sectional view of the continuous percutaneous micropin sensor of Figure 10 one embodiment of the invention.In the present embodiment
One micropin group 22 is formed by the first thin slice 222 and the second thin slice 224 are stacked, can for example apply a stamping press in the first thin slice
222 and second thin slice 224 surrounding with combine both.Second micropin group 24 has the first thin slice 242.3rd micropin group 26
Only there is the first thin slice 262.Since the present invention has a flexible gasket 30, during operation can with the muscle profile syntype of user,
It is in close contact.
Then, please refer to Fig.1 1, Figure 11 be Figure 10 partial schematic sectional view, wherein lactic acid sensing ferment be coated on it is micro-
To form a lactic acid ferment layer on the bur of pin.Specifically, lactic acid sensing ferment is coated on the inner surface of bur, bur
Outer surface on be coated with the drug of anti-skin allergy.One surface scribbles the continuous percutaneous micropin of the micropin of lactic acid sensing ferment
Sensor can be used to the lactic acid concn in detection skin surface, and this concentration can be as one of index for judging physiological status.
Please refer to Fig.1 the close-up schematic view that 2, Figure 12 is bur in Figure 11.Since ampere meter electrochemical method is general
Than less selective, many common chaff interferents are present in blood plasma, are significantly most ascorbic acid (ascorbic acid),
It can import among signal.In order to realize high lactic acid selectivity, in being initially formed a such as sulfonation of semi-permeable membrane 941 on the surface of electrode
Polyether ethersulfone/polyether sulfone (SPEES/PES) film forms lactic acid ferment layer 943 on semi-permeable membrane 941.Negatively charged
The impermeable ascorbic acid of SPEES/PES height, while very small neutral molecule such as hydrogen peroxide is easily allowed to permeate again.Also, in order to
The drug of lactic acid sensing ferment or anti-skin allergy is avoided to be subject to environmental pollution, it can be in 943 or anti-skin allergy of lactic acid ferment layer
Drug surface on form a protective layer 945, protective layer 945 is, for example, epoxy resin-Polyurethane formyl resin (Epoxy-
PU) film.The epoxy resin of outer layer-Polyurethane formic acid base resin film has multiple functions：(i) protect lactic acid ferment layer from measurement
The influence of environment, (II) prevent the leaching of lactic acid sensing ferment and (iii) from providing biofacies between lactic acid measuring equipment and tissue
The interface of appearance.In addition, epoxy resin-Polyurethane formic acid base resin film limits lactic acid simultaneously diffuses to sensing surface, but allow to allow
Oxygen molecule free diffusing contributes to enzymatic reaction, is possibly realized so that the range of linearity is widened, and is limited in order to avoid being reacted by enzyme kinetics
System.
Figure 13 is the partial schematic sectional view of Figure 10, and wherein lactic acid sensing ferment is coated on test-paper.The present embodiment
It is with the difference of embodiment illustrated in fig. 10, the first micropin group 22 of the present embodiment is as the instrument for extracting interstitial fluid, bur
It goes up and is not coated with lactic acid sensing ferment, lactic acid sensing ferment is the surface for being coated on the test-paper positioned at 22 lower section of the first micropin group
On.In the present embodiment, test-paper is placed between the first micropin group 22 and substrate 10, and test-paper includes a conductive layer 92 and position
In being coating lactic acid sensing ferment on multiple test zones 94 on conductive layer 92, those test zones 94 to form a lactic acid ferment
Plain layer, and align with the perforation 2222 in the first micropin group 22.The present embodiment is to define those thereon using resin sheet 96
Test zone 94.In addition, the first micropin group 22 is by an adhesion layer 98 and test paper chip bonding.
Then, the close-up schematic view that 4, Figure 14 is test piece paper in Figure 13 is please referred to Fig.1.Similarly, it is high in order to realize
Lactic acid selectivity, in being initially formed a semi-permeable membrane 941 such as sulfonated polyether ether sulfone/polyether sulfone (SPEES/PES) on the surface of electrode
Film forms lactic acid ferment layer 943 on semi-permeable membrane 941.The impermeable ascorbic acid of SPEES/PES height, while easily allow again non-
Often small neutral molecule such as hydrogen peroxide permeates.Also, in order to avoid the drug that lactic acid senses ferment or anti-skin allergy is subject to ring
Border is polluted, and a protective layer 945, protective layer 945 can be formed on the surface of the drug of 943 or anti-skin allergy of lactic acid ferment layer
Epoxy resin-Polyurethane formyl resin (Epoxy-PU) film in this way.Lactic acid senses ferment in addition to lactate oxidase, also may be used
Selected from lactic dehydrogenase (lactate dehydrogenase), cytochrome b 2 (Cytochrome b2), lactate mono-oxygenase
(lactate monooxygenase) or catalase (hydrogen peroxidase) etc..
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding changes and deformation, but these corresponding changes and change in accordance with the present invention
Shape should all belong to the protection domain of appended claims of the invention.
Claims (14)
1. a kind of method of training adjustment measures people by the lactic acid measuring equipment comprising a continuous percutaneous micropin sensor
Lactic acid concn in body, which is characterized in that the method for training adjustment comprises the following steps：
The lactic acid concn value in user's tissue is measured using a continuous percutaneous micropin sensor；
Compare the lactic acid concn value and a benchmark preset value；And
If the lactic acid concn value then notifies user to reduce exercise intensity higher than the benchmark preset value, if otherwise the lactic acid is dense
Angle value then notifies user to increase exercise intensity less than the benchmark preset value；
The continuous percutaneous micropin sensor includes：
One substrate；
One microneedle unit, including at least the one first micropin group and one second micropin group being arranged on the substrate, first micropin
Group is as working electrode, which is used as with reference to electrode, and each micropin group includes at least a micropin, the first micropin group
Comprising multiple thin slices, a perforation is at least set, which is provided with a bur, wherein wearing on a thin slice on each thin slice
Hole is passed through for the bur of the perforated edge of relative position on remaining thin slice, and those burs are disconnected from each other；
One signal processing unit is arranged on the substrate and is electrically connected with the first and second micropins group；And
One power supply unit, supply working power give a monitoring system.
2. the method for training adjustment according to claim 1, which is characterized in that the first micropin group is thin by one first
Piece and one second thin slice are stacked, and one first perforation is at least set on first thin slice, which is provided with one
One second perforation is at least set on the first bur and second thin slice, which is provided with one second bur, this
First perforation of two burs through relative position on first thin slice is opposite with first bur.
3. the method for training adjustment according to claim 1, which is characterized in that the first micropin group is thin by one first
Piece, one second thin slice and one the 3rd thin slice are stacked, and one first perforation, first punching edge are at least set on first thin slice
Edge is provided with one first bur, and one second perforation is at least set on second thin slice, which is provided with one second
One the 3rd perforation is at least set on bur and the 3rd thin slice, and the 3rd perforated edge is provided with one the 3rd bur, this is second prominent
Thorn and the 3rd bur pass through first perforation on first thin slice with first bur in triangular pyramidal.
4. the method for training adjustment according to claim 1, which is characterized in that the first micropin group is thin by one first
Piece, one second thin slice, one the 3rd thin slice and one the 4th thin slice are stacked, and one first perforation is at least set on first thin slice,
First perforated edge is provided with one first bur, and one second perforation, second perforated edge are at least set on second thin slice
One second bur is provided with, one the 3rd perforation is at least set on the 3rd thin slice, and it is prominent that the 3rd perforated edge is provided with one the 3rd
One the 4th perforation is at least set in thorn and the 4th thin slice, the 4th perforated edge is provided with one the 4th bur, second bur,
3rd bur and the 4th bur pass through first perforation on first thin slice with first bur in corner taper.
5. the method for training according to claim 1 adjustment, which is characterized in that the first and second micropins group it is micro-
Pin is formed by punching press or etch process.
6. the method for training adjustment according to claim 1, which is characterized in that scribbled on the inner surface of those burs
Lactic acid senses ferment.
7. the method for training adjustment according to claim 1, which is characterized in that the continuous percutaneous micropin sensor is more
It comprising a test-paper, is placed between the first micropin group and the substrate, which includes a conductive layer and positioned at the conduction
Multiple test zones on layer, are coated with lactic acid sensing ferment on those test zones, and with it is each thin in the first micropin group
The perforation alignment of on piece.
8. the method for training adjustment according to claim 1, which is characterized in that the material of those burs is selected from not
Become rusty steel, nickel, nickel alloy, titanium, titanium alloy, carbon nanotubes or silicon materials.
9. the method for training adjustment according to claim 1, which is characterized in that the material of those burs is resin,
And in metal of the surface deposition with biocompatibility.
10. the method for training adjustment according to claim 1, which is characterized in that the benchmark preset value is Lactate Threshold
Value, maximum lactic acid steady-state value, blood lactase acid accumulation initial value.
11. the method for training adjustment according to claim 1, which is characterized in that the benchmark preset value is user
Personal anaerobic threshold.
12. a kind of lactic acid measuring equipment, which is characterized in that include：
One substrate；
One microneedle unit, including at least the one first micropin group and one second micropin group being arranged on the substrate, first micropin
Group is as working electrode, which is used as with reference to electrode, and each micropin group includes at least a micropin, the first micropin group
Comprising multiple thin slices, a perforation is at least set, which is provided with a bur, wherein wearing on a thin slice on each thin slice
Hole is passed through for the bur of the perforated edge of relative position on remaining thin slice, and those burs are disconnected from each other；
One signal processing unit is arranged on the substrate and is electrically connected with the first and second micropins group；
One comparator, for comparing the lactic acid measuring value and a benchmark preset value from the signal processing unit；And
One power supply unit, supply working power give a monitoring system.
13. lactic acid measuring equipment according to claim 12, which is characterized in that the micropin of the first and second micropins group by
It is formed by punching press or etch process.
14. lactic acid measuring equipment according to claim 12, which is characterized in that further include a test-paper, be placed in this
The test-paper includes a conductive layer and multiple test zones on the conductive layer between one micropin group and the substrate, those surveys
It tries to be coated with lactic acid sensing ferment on region, and aligns with the perforation in the first micropin group.
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| CN109688923B (en) * | 2016-09-09 | 2022-04-19 | 日本电信电话株式会社 | Lactic acid operation threshold estimation device and lactic acid operation threshold estimation method |
| TWI666034B (en) * | 2017-03-31 | 2019-07-21 | 全康科技股份有限公司 | Transdermal microneedle array patch |
| TWI634528B (en) * | 2017-08-29 | 2018-09-01 | 國立臺北商業大學 | Computer program product for assessing cognitive ability |
| TWI635469B (en) * | 2017-08-29 | 2018-09-11 | 國立臺北商業大學 | Computer program product for reflex training |
| TWI730504B (en) * | 2019-11-19 | 2021-06-11 | 奇異平台股份有限公司 | Percutaneous microneedle monitoring system |
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