CN105455855A - Lactic acid measuring device and exercise training adjustment method - Google Patents
Lactic acid measuring device and exercise training adjustment method Download PDFInfo
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- CN105455855A CN105455855A CN201410448944.5A CN201410448944A CN105455855A CN 105455855 A CN105455855 A CN 105455855A CN 201410448944 A CN201410448944 A CN 201410448944A CN 105455855 A CN105455855 A CN 105455855A
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Abstract
The invention discloses a lactic acid measuring device and an exercise training adjustment method. According to the exercise training adjustment method, lactic acid concentration in a human body is measured through the lactic acid measuring device containing a continuous percutaneous microneedle sensor, and the exercise training adjustment method comprises the following steps that a lactic acid concentration value in human tissue of a user is measured by means of the continuous percutaneous microneedle sensor; the lactic acid concentration value and a base preset value are compared; if the lactic acid concentration value is larger than the base preset value, the user is informed to lower the exercise intensity, and if the lactic acid concentration value is smaller than the base preset value, the user is informed to enhance the exercise intensity. The invention further provides the lactic acid measuring device.
Description
Technical field
The present invention relates to the method for a kind of training adjustment, particularly a kind of method adjusted for training by lactic acid concn in percutaneous micropin sensor measurement human body.
Background technology
In motion, the main carbohydrate that uses is as energy source, and carbohydrate produce power is divided into aerobic and anaerobic two kinds of modes, and carbohydrate decomposes under anaerobic situation, except meeting produce power, also can produce lactic acid.When exercise intensity is lower, the main mode produce power using aerobic, anaerobic participates in less, and therefore the speed of lactic acid generation is not high, and human body can be easy to metabolism and fall, so lactic acid can not be accumulated in health and blood.Along with the increase of exercise intensity, improve without oxygen energy usage rate, the speed that lactic acid produces also speeds thereupon, the speed of human body removing lactic acid can be unable to catch up with the speed of lactic acid generation gradually, therefore lactic acid will start slowly to pile up in the body, under this exercise intensity, the Lactate in human body is called as lactic acid threshold value (LactateThreshold).On the time point of the lactic acid threshold value between aerobic metabolism and anaerobic metabolism, the lactate level in human body can keep balance.If athletic lactic acid threshold value and relevant cardiac rate are known, can according to above data optimization to this athletic training.
Lactic acid is tissue oxygenation (oxygenation) most important biomarker, and therefore to physical culture, the application of health care is very important.Lactic acid concn provides the information of anaerobic threshold, and this information is very important to formulation endurance exercise training program.The commercial interest of current lactic acid measuring equipment drives and comes from motion, body-building, milk product and national defense industry.
The normal blood lactate concentration of static rest is in the scope of 0.5-2mM, but when reaching anaerobic threshold or injured initiation hemorrhagic shock when strenuous exercise, cell oxygen-supplying amount has become in limited time, and lactic acid can increase sharply.Measure lactic acid to contribute to identifying that fatigue reaction (to athlete, other physical culture personage and soldier are even more important) and the personalized training flow process of design are to athlete.Lactic acid also has clinical meaning to when many Intensive Care Therapies, and such as, lactic acidosis, is especially embodied in shock state.
Having physiology of exercise scholar to think in motion and the change of blood lactase acid after motion, is lactic acid generating rate in the tissues such as skeletal muscle, enters the performance balanced between disappearance of lactic acid speed in the speed of blood and blood.Therefore, exercise intensity, persistent period, the metabolism between each histoorgan, all relevant with the concentration of blood lactase acid, at the volley with motion after, can continuous detecting blood lactase acid the Come impact of human body and rule thereof when understanding motion.The utilization of test on motion instruction Practice of blood lactase acid, the content of instruction Practice is a quite complicated job, plan in order to a more perfect instruction Practice course and instruction Practice will be provided, religion Practice needs to find out one to the optimal quantity of motion of athlete and stimulation usually, lactic acid is at blood level, and rest value is about 1mM, can be used as the foundation judging exercise intensity, when lactic acid concn reaches 4.0mM in blood, can be used as judgement anaerobic threshold.Prior document is pointed out, when doing dynamically fierce motion, in blood, lactic acid concn has high to 30 μMs/g.Separately there is document to point out, when intramuscular lactic acid concn reaches 20-25 μM/g, make people's meeting completely tired and cannot move.
The principal element that in motion, lactic acid produces is to have again document to point out: one, the load intensity of motion; Two, the muscle quantities of Ginseng and motion; Three, the time of motion continuation.The concentration number that lactic acid produces, then relevant with the percentage ratio of aerobic in motor process (aerobic) and the metabolism of anaerobic (anaerobic) system capacity.Different energy systems exports, and affects the change of its concentration for blood lactase acid value during human motion.When being main supply energy source with phosphoric acid system, blood lactate concentration is less, is in general no more than 4mM; If when being main supplying energy source with glycolysis system, can up to 15mM; At 4mM when being main supplying energy with aerobic energy system.Therefore, after the training of varying strength, the accumulation situation of blood lactase acid has significant diversity.Lactic acid is piled up and is had two kinds of meanings, and a kind of is because accelerate the effect of glycolysis, produces a large amount of energy and uses for muscle contraction; Another represents that the degree of acidify in muscle improves, and original aerobic metabolism systemic-function is not applied and used, and the full anaerobic glycolysis that relies is used to provide energy.In addition, have physiology of exercise scholar to think under stable Like state, the situation that blood lactase acid is piled up can as one of biological parameter judging sports load.
The modal reason that lactic acid is piled up is anoxia (hypoxia) (low tissue and blood oxygenation).The standard method that laboratory measures lactic acid concn is outward discrete blood sampling and measures through lactic acid reagent paper.But this helps little to dynamic change or information of forecasting, and may cause lacking an accurate concentration reestablishing.Carry out when in fact discrete sampling is not convenient for endurance activities.In addition, cannot directly lay down a definition to result.
The lactic acid measuring equipment of direct implantable intravascular is not a practical selection, and reason to cause the risk of thrombosis and thromboembolism, the interaction between causing by blood/lactic acid measuring equipment, and allows and produce dynamic variation by the blood flow of local.The measurement of percutaneous tissue liquid provides a safer option, any adverse effect can be localized.And the lactic acid in tissue, also can provide more relevant information to the oxygen supply of local, thus detect local tissue hypoxia, instead of by hemanalysis obtain the meansigma methods of health.
Use lactic acid measuring equipment to measure Lactate, be in athlete in different motion intensity time, blood sampling is analyzed.The method of medical personnel's sampling blood, mostly adopt and have an acupuncture treatment and wear out horny layer, extract blood to detect to carry out analysis, but this kind destroys the sampling method of skin surface, except easily making athlete's feels pain, and then germinating is repelled outside sense, a large amount of microorganisms of skin surface, also easy in the destroyed situation of skin surface, enter human body and then infection.In order to reduce the risk of infection, needing high sanitary standard, thus making the method become complicated and expensive.
In addition, general non-intrusion type measures the method for lactic acid is be in fashion Kang Keni test (Conconitest) being used in sports medical science gradually.In the method, testee is on the athletic tracks of 400 meters, and run with a predetermined speed for first 200 meters, after 200 meters, testee segmentation increases rhythm, such as, distinguish 0.5km/h.In each 200 meters of mark of athletic tracks, testee, after respectively circus movement runway, is noted his cardiac rate at that time, and is barked out data to assistant.Testee is run on athletic tracks, until he arrives strength limits, means that he cannot gather way again.
For the estimation of test, cardiac rate is mapped with two dimension (X-Y) facing to relevant running velocity.Find result by this: in aerobic scope, provide lower strength, cardiac rate is almost linear with running velocity.This means that the strength equal proportion that cardiac rate produces along with testee increases.The threshold value place that this rule is being converted to anaerobic metabolism is broken.In the anaerobic scope of high output, cardiac rate only increases a little along with the strength increased further or running velocity.Thus the relation that cardiac rate is relevant to running velocity shows clear, the sharp-pointed discontinuity transitting to the anaerobic scope of high output from low aerobic scope of exerting oneself, and determines lactic acid threshold value by this.Cardiac rate feature for lactic acid threshold value and relevant running velocity can simply read from X-Y figure.
But Kang Keni test and comparison is complicated, and almost cannot perform when not having assistant.Again, control the capacity of his running velocity due to weather and testee, the lactic acid threshold value that Kang Keni test measures is more inaccurate.
In sum, need the lactic acid measuring equipment that a kind of low invasive can be used in continuous percutaneous carbon dioside monitoring tissue, be supplied to motion, body-building, like personage with the use of national defense industry, particularly athlete and motion, make it keep muscle not ache, and adjust its exercise intensity, reach the fitness goals effectively do not injured.
Summary of the invention
An object of the present invention, be a kind of method providing training to adjust, carry out skin penetrating by the percutaneous sensor with micropin, low invasive puncture effectively can alleviate the pain of user, reaches again sampling tissue fluid to measure the object of lactic acid concn value in human body simultaneously.According to comparing of lactic acid concn value and a benchmark preset value, help athlete or the personage that is keen on sports to adjust exercise intensity and frequency, and then reach the most effective training.
In order to reach above-mentioned object, the invention provides the method for a kind of training adjustment, measure lactic acid concn in human body by the lactic acid measuring equipment comprising a continuous percutaneous micropin sensor, the method for this training adjustment comprises the following steps: use the lactic acid concn value in continuous percutaneous micropin sensor measurement user tissue; Relatively lactic acid concn value and a benchmark preset value; And if higher than benchmark preset value, lactic acid concn value then notifies that user reduces exercise intensity, otherwise if lower than benchmark preset value, lactic acid concn value then notifies that user increases exercise intensity, guarantee that user's muscle is not ached, be enough to omnidistance match.Estimate the distance completed and the distance waited according to GPS or pedometer etc. further, effectively can adjust safe lactic acid concn value and the change rate of concentration of its safety, to improve the achievement of athlete's.Same, for various ball match or the match of various team sport, the physical ability situation of individual, the need of rest, or sustainablely to complete, lactic acid concn value and its change rate of concentration are all can as the foundation of effective reference.
Another object of the present invention, be to provide a kind of lactic acid measuring equipment, carry out skin penetrating by the percutaneous sensor with micropin, low invasive puncture effectively can alleviate the pain of user, reaches again sampling tissue fluid to measure the object of lactic acid concn value in human body simultaneously.
In order to reach above-mentioned object, the present invention is also provided as a kind of lactic acid measuring equipment, lactic acid measuring equipment comprises continuous percutaneous micropin sensor and a comparator, and comparator is for comparing lactic acid production measured value from the signal processing unit of continuous percutaneous micropin sensor and a benchmark preset value.The running of lactic acid measuring equipment of the present invention generates electroactive hydrogen peroxide based on lactic acid sensing ferment, and then produce Ampere currents at a polarization platinum electrode, depends on interior adventitia and realize selectivity and tissue biocompatibility.Lactic acid measuring equipment of the present invention can be fully calibrated, uncommon in receptor chaff interference (as ascorbic acid and acetaminophen) impact.There is the range of linearity wide, cover whole physiological range (can 25mM be reached), with the high selectivity to lactic acid.
Blood lactase acid heap initial value (theonsetofbloodlactateaccumulation, OBLA) be the exercise intensity corresponding to 4mM lactic acid concn, it represents the maximum working load of lactic acid concn under steady statue, and corresponds to and be transitioned into more serious exercise intensity from allowable load.Different research shows the importance of OBLA in long-distance running performance, and it is also considered to a sensitive indicator of training the adaptation caused, and the responsive identification beacon between elite and outstanding athlete.
The method of training of the present invention or match adjustment, the strategy of adjustment exercise intensity is with lactic acid threshold value (lactatethreshold substantially, or anaerobic threshold LT), maximum lactic acid steady-state value (maximallactatesteadystate, MLSS), or blood lactase acid heap initial value (theonsetofbloodlactateaccumulation, OBLA) as benchmark preset value, when lactic acid production measured value exceedes this benchmark preset value, and lactic acid changing value increases, then exercise intensity can reduce.Whether reduce to observe lactic acid changing value, until lactic acid production measured value gets back to benchmark preset value.If exceed benchmark preset value, but still maintain exercise intensity, and lactic acid changing value is close to zero, then represent, the physical ability situation of athlete today is good, can maintain exercise intensity now.Whether further, athlete slightly can increase exercise intensity, and observe lactic acid production measured value and continue to increase, and is still increased to an equilibrium point, namely remains unchanged.Through so effective training, athlete, at identical lactic acid preset value, can break through exercise intensity in the past, such as, run, then its speed can be accelerated; Such as swim, then its swimming rate can be accelerated, and therefore games results all can improve.
Another embodiment, with individual anaerobic threshold (individualanaerobicthreshold, IAT) be a preset reference value, IAT be defined as lactic acid concn than lactic acid threshold value (lactatethreshold, LT) have a net increase of long 1.5mM time velocity.
According to continuous percutaneous micropin sensor of the present invention, this micropin sensor comprises: substrate, microneedle unit, signal processing unit and power subsystem.Microneedle unit at least comprises the first micropin group be arranged in as working electrode on substrate, and as the second micropin group with reference to electrode, each micropin group at least comprises a micropin, first micropin group comprises at least one thin slice, each thin slice at least arranges a perforation, perforated edge is provided with a bur, and the perforation wherein on a thin slice supplies the bur of the perforated edge of relative position on remaining thin slice to pass, and those burs are disconnected from each other.
Compared to prior art, the present invention uses the lactic acid concn value in a continuous percutaneous micropin sensor measurement user tissue, and low invasive puncture can reduce the risk of infection.And, the micropin sensor that method of the present invention uses can easyly be dressed, lactic acid concn value in accurate measurement tissue, be conducive to the physiological parameter that user knows oneself more accurately, help adjustment exercise intensity and frequency, and then reach the most effective training.
Describe the present invention below in conjunction with the drawings and specific embodiments, but not as a limitation of the invention.
Accompanying drawing explanation
The flow chart of steps of the method for Fig. 1 one embodiment of the invention training adjustment.
The explosive decomposition figure of Fig. 2 one embodiment of the invention continuous percutaneous micropin sensor;
The explosive decomposition figure of one embodiment of the invention continuous percutaneous micropin sensor of the different view direction of Fig. 3 and Fig. 2;
The schematic diagram of Fig. 4 one embodiment of the invention microneedle unit;
The structure partial top view of Fig. 5 one embodiment of the invention working electrode micropin group;
The structure partial top view of Fig. 6 another embodiment of the present invention working electrode micropin group;
The structure partial top view of Fig. 7 another embodiment of the present invention working electrode micropin group;
The structure partial top view of Fig. 8 another embodiment working electrode micropin group again of the present invention;
The combination schematic appearance of Fig. 9 one embodiment of the invention continuous percutaneous micropin sensor;
The combination cross-sectional schematic of Figure 10 one embodiment of the invention continuous percutaneous micropin sensor;
Figure 11 is the partial schematic sectional view of Figure 11, and wherein lactic acid sensing ferment is coated on the bur of micropin;
Figure 12 is the close-up schematic view of bur in Figure 11;
Figure 13 is the partial schematic sectional view of Figure 10, and wherein lactic acid sensing ferment is coated on test-paper; And
Figure 14 is the close-up schematic view of test piece paper in Figure 13.
Detailed description of the invention
Detailed description for the present invention and technology contents, coordinate accompanying drawing to be described as follows, but appended accompanying drawing only provides reference and explanation use, is not used for being limited the present invention.
Please refer to Fig. 1, the method of Fig. 1 one embodiment of the invention training adjustment, lactic acid concn in human body is measured by the lactic acid measuring equipment comprising a continuous percutaneous micropin sensor, the method of this training adjustment, comprise the following steps: step S10, use the lactic acid concn value in a continuous percutaneous micropin sensor measurement user tissue; Step S12, compares lactic acid concn value and a benchmark preset value; And step S14, if higher than benchmark preset value, lactic acid concn value then notifies that user reduces exercise intensity, otherwise if lower than benchmark preset value, lactic acid concn value then notifies that user increases exercise intensity.Benchmark preset value in step S12 is the basic physiological data with reference to common people, such as age, sex, height and body weight etc., the lactic acid concn value of acquisition.
In one embodiment of the invention, lactic acid measuring equipment comprises continuous percutaneous micropin sensor and a comparator (not shown), and comparator is for comparing lactic acid production measured value from this signal processing unit and a benchmark preset value.
Please refer to Fig. 2 and Fig. 3, Fig. 2 and Fig. 3 is watched the explosive decomposition figure of one embodiment of the invention continuous percutaneous micropin sensor respectively by different directions.Continuous percutaneous micropin sensor of the present invention comprises: substrate 10, microneedle unit 20, pliability pad 30, signal processing unit 41, power subsystem 43 and enclosing cover 50, wherein signal processing unit 41 and power subsystem 43 are arranged on circuit board 40.
According to one embodiment of the invention, microneedle unit 20 comprises to be arranged in and substrate 10 covers sulfonated polyether ether sulfone/poly (ether sulfone) film and lactic acid sensing ferment as the compatible conductive material of the first micropin group 22 of working electrode, capping oxidation iridium or other biological as the second micropin group 24 with reference to electrode, and covers the three micropin group 26 of platinum as antielectrode.Those micropins of first micropin group 22 can be such as that array format is on substrate 10.Pliability pad 30 has an opening 32 to pass through for microneedle unit 20, and microneedle unit 20 is electrically connected with the electric contact 42,44,46 on conductive pole 21,23,25 and circuit board 40.Because the present invention has pliability pad 30, during operation can with the muscle profile syntype of user, close contact.When micropin group inserts subcutaneous tissue, and polarizing voltage is applied between electrode, the lactic acid lactic acid sensed in ferment and tissue fluid reacts and generates electroactive hydrogen peroxide (H2O2), and then produces Ampere currents, this electric current and the proportional relation of lactic acid concn at working electrode.
Signal processing unit 41 and microneedle unit 20 are electrically connected to receive the concentration of target molecules that micropin sense, and after computing judgement, information being converted to a sensing signal, is also a kind of signal that can reflect user physiological status instantly.Power subsystem 43 is that supply power for operation is to continuous percutaneous micropin sensor of the present invention.
Please refer to Fig. 4, the schematic diagram of Fig. 4 one embodiment of the invention microneedle unit.First micropin group 22 is by the first thin slice 222 and the second thin slice 224 is stacked forms, first thin slice 222 is at least arranged one first perforation 2222, these the first perforation 2222 edges are provided with one first bur 2224, and second thin slice 224 is at least arranged one second perforation 2242, it is relative with the first bur 2224 through the first perforation 2222 of relative position on the first thin slice 222 that second perforated edge is provided with one second bur 2244, second bur 2244.In addition, the second thin slice 224 edge of the first micropin group 22 can arrange barb 2246 engage with the hole 102 on substrate 10.In an embodiment, the second thin slice 224 edge of the first micropin group 22 can be arranged and lead handle 2248 and insert slot 104 on substrate 10, be electrically connected by circuit and conductive pole 21.
In like manner, the second micropin group 24 also has and the first thin slice 242, first thin slice 242 at least arranges one first perforation the 2422, first perforated edge and be provided with one first bur 2424.In addition, the first thin slice 242 edge of the second micropin group 24 can arrange barb 2426 engage with the hole 102 on substrate 10.In an embodiment, the first thin slice 242 edge of the second micropin group 24 can be arranged and lead handle 2428 and insert slot 104 on substrate 10, be electrically connected by circuit and conductive pole 23.
In like manner, the 3rd micropin group 26 also has and the first thin slice 262, first thin slice 262 at least arranges one first perforation 2622, first perforation 2622 edges and be provided with one first bur 2624.In addition, the first thin slice 262 edge of the 3rd micropin group 26 can arrange barb 2626 engage with the hole 102 on substrate 10.In an embodiment, the first thin slice 262 edge of the 3rd micropin group 26 can be arranged and lead handle 2628 and insert slot 104 on substrate 10, be electrically connected by circuit and conductive pole 25.
One embodiment of the invention, the micropin of the first micropin group 22, second micropin group 24 and the 3rd micropin group 26 is formed by punching press or etch process.The material of those burs is selected from rustless steel, nickel, nickel alloy, titanium, titanium alloy, CNT or silicon materials.The material of those burs also can be resin is such as Merlon, polymethacrylic acid copolymer, ethylene/vinyl acetate copolymer, Teflon or polyesters, and has the metal of biocompatibility in surface deposition.The height of those burs is 300-600 micron, base widths is 150-450 micron.The point of those burs be spaced apart 500-3000 micron.
Please refer to Fig. 5 to Fig. 8.The structure partial top view of Fig. 5 one embodiment of the invention working electrode micropin group.First micropin group 22 is by the first thin slice 222 and the second thin slice 224 is stacked forms, first thin slice 222 is at least arranged one first perforation 2222, first perforation 2222 edges are provided with one first bur 2224, and second thin slice 224 is at least arranged one second perforation 2242, it is relative with the first bur 2224 through the first perforation 2222 of relative position on the first thin slice 222 that second perforated edge is provided with one second bur 2244, second bur 2244.
The structure partial top view of Fig. 6 another embodiment of the present invention working electrode micropin group.First micropin group 22 is by the first thin slice 222, second thin slice 224 and the 3rd thin slice 226 is stacked forms, first thin slice 222 is at least arranged the first perforation 2222, first perforation 2222 edges are provided with one first bur 2224, second thin slice 224 is at least arranged one second perforation 2242, second perforation 2242 edges are provided with one second bur 2244, and the 3rd thin slice 226 is at least arranged one the 3rd perforation 2262, 3rd perforation 2262 edges are provided with one the 3rd bur 2264, second bur 2244 and the 3rd bur 2264 are positive triangular pyramidal through the first perforation 2222 on the first thin slice 222 and the first bur 2224.
The structure partial top view of Fig. 7 another embodiment of the present invention working electrode micropin group.First micropin group 22 be by the first thin slice 222, second thin slice 224, the 3rd thin slice 226 is stacked forms, wherein the first thin slice 222 is at least arranged one first perforation 2222, first perforation 2222 edges and is provided with one first bur 2224; Second thin slice 224 is at least arranged one second perforation 2242, second perforation 2242 edges and be provided with one second bur 2244; And the 3rd thin slice 226 is at least arranged one the 3rd perforation 2262,3rd perforation 2262 edges are provided with one the 3rd bur 2264, second bur 2244 and the 3rd bur 2264 are passed the first perforation 2222 and the first bur 2224 arranged adjacent on the first thin slice 222, in the taper of isosceles right angle trigonometry.
The structure partial top view of Fig. 8 another embodiment working electrode micropin group again of the present invention.First micropin group 22 is by the first thin slice 222, second thin slice 224, 3rd thin slice 226 and the 4th thin slice 228 is stacked forms, first thin slice 222 is at least arranged one first perforation 2222, first perforation 2222 edges are provided with one first bur 2224, second thin slice 224 is at least arranged one second perforation 2242, second perforation 2242 edges are provided with one second bur 2244, 3rd thin slice 226 is at least arranged one the 3rd perforation 2262, 3rd perforation 2262 edges are provided with on one the 3rd bur 2264 and the 4th thin slice 228 and at least arrange one the 4th perforation 2282, 4th perforation 2282 edges are provided with one the 4th bur 2284, second bur 2244, 3rd bur 2264 and the 4th bur 2284 are corner taper through the first perforation 2222 on the first thin slice 222 and the first bur 2224.
In shown in Fig. 5 to Fig. 8 four embodiment, each bur 2224 of first micropin group 22 comprises point 2221 and a substrate 2223, and the top of those points of this micropin that the perforation wherein on a thin slice is formed after the bur of the perforated edge of relative position on remaining thin slice passes is not at sustained height.Or, can according to the order of those thin slice overlaps, design the height of its bur in advance, the top of those points of this micropin that the perforation on a wherein thin slice is formed after the bur of the perforated edge of relative position on remaining thin slice passes has sustained height.
Then, please refer to Fig. 9 and Figure 10.The combination schematic appearance of Fig. 9 one embodiment of the invention continuous percutaneous micropin sensor.The combination cross-sectional schematic of Figure 10 one embodiment of the invention continuous percutaneous micropin sensor.The first micropin group 22 in the present embodiment is by the first thin slice 222 and the second thin slice 224 is stacked forms, and such as can apply the surrounding of a stamping press in the first thin slice 222 and the second thin slice 224 with both combining.Second micropin group 24 only has the first thin slice 242.3rd micropin group 26 also only has the first thin slice 262.Because the present invention has pliability pad 30, during operation can with the muscle profile syntype of user, close contact.
Then, please refer to Figure 11, Figure 11 is the partial schematic sectional view of Figure 10, wherein lactic acid sensing ferment be coated on micropin bur on to form a lactic acid ferment layer.Specifically, lactic acid sensing ferment is coated on the inner surface of bur, the outer surface of bur is coated with the medicine of anti-skin allergy.Surface scribbles the continuous percutaneous micropin sensor of the micropin of lactic acid sensing ferment, can in order to the lactic acid concn in detection of skin top layer, and this concentration can be used as one of index judging physiological status.
Please refer to Figure 12, Figure 12 is the close-up schematic view of bur in Figure 11.Because ammeter electrochemical method is generally than less selectivity, many common chaff interferences are present in blood plasma, are significantly ascorbic acid (ascorbicacid), among meeting lead-in signal.In order to realize high lactic acid selectivity, on the surface of electrode, first forming a semipermeable membrane 941 such as sulfonated polyether ether sulfone/polyether sulfone (SPEES/PES) film, then on semipermeable membrane 941, form lactic acid ferment layer 943.The highly not saturating ascorbic acid of electronegative SPEES/PES, easily allows again very little neutral molecule such as hydrogen peroxide permeate simultaneously.Again; in order to avoid the medicine of lactic acid sensing ferment or anti-skin allergy is subject to environmental pollution; can on the surface of the medicine of lactic acid ferment layer 943 or anti-skin allergy formation one protective layer 945, protective layer 945 is such as epoxy resin-Polyurethane formyl resin (Epoxy-PU) film.Outer field epoxy resin-Polyurethane formyl resin molding has several functions: (i) protects lactic acid ferment layer from the impact of measurement environment; (II) prevents lactic acid from sensing the leaching of ferment, and (iii) provides biocompatible interface between lactic acid measuring equipment and tissue.In addition, epoxy resin-Polyurethane formyl resin molding diffuses to sensitive surface with limit lactic acid, but allows to allow oxygen molecule free diffusing contribute to enzymatic reaction, thus the range of linearity is widened become possibility, in order to avoid by enzyme kinetics reaction restriction.
Figure 13 is the partial schematic sectional view of Figure 10, and wherein lactic acid sensing ferment is coated on test-paper.The present embodiment and difference embodiment illustrated in fig. 10 are, the first micropin group 22 of the present embodiment is the instrument as extraction interstitial fluid, bur is not coated with lactic acid sensing ferment, lactic acid sensing ferment is coated on the surface of the test-paper be positioned at below the first micropin group 22.In the present embodiment, test-paper is placed between the first micropin group 22 and substrate 10, multiple test zones 94 that test-paper comprises a conductive layer 92 and is positioned on conductive layer 92, those test zones 94 are coating lactic acid senses ferment to form a lactic acid ferment layer, and align with the perforation 2222 in the first micropin group 22.The present embodiment uses resin sheet 96 to define those test zones 94 thereon.In addition, the first micropin group 22 engages with test-paper by an adhesion layer 98.
Then, please refer to Figure 14, Figure 14 is the close-up schematic view of test piece paper in Figure 13.In like manner, in order to realize high lactic acid selectivity, on the surface of electrode, first forming a semipermeable membrane 941 such as sulfonated polyether ether sulfone/polyether sulfone (SPEES/PES) film, then on semipermeable membrane 941, form lactic acid ferment layer 943.The highly not saturating ascorbic acid of SPEES/PES, easily allows again very little neutral molecule such as hydrogen peroxide permeate simultaneously.Again; in order to avoid the medicine of lactic acid sensing ferment or anti-skin allergy is subject to environmental pollution; can on the surface of the medicine of lactic acid ferment layer 943 or anti-skin allergy formation one protective layer 945, protective layer 945 is such as epoxy resin-Polyurethane formyl resin (Epoxy-PU) film.Lactic acid sensing ferment, except Lactate Oxidase, also can be selected from lactic acid dehydrogenase (lactatedehydrogenase), cytochrome b 2 (Cytochromeb2), lactate mono-oxygenase (lactatemonooxygenase) or catalase (hydrogenperoxidase) etc.
Certainly; the present invention also can have other various embodiments; when not deviating from the present invention's spirit and essence thereof; those of ordinary skill in the art are when making various corresponding change and distortion according to the present invention, but these change accordingly and are out of shape the protection domain that all should belong to the claim appended by the present invention.
Claims (15)
1. a method for training adjustment, measures lactic acid concn in human body by the lactic acid measuring equipment comprising a continuous percutaneous micropin sensor, it is characterized in that, the method for this training adjustment comprises the following steps:
Use the lactic acid concn value in a continuous percutaneous micropin sensor measurement user tissue;
Relatively this lactic acid concn value and a benchmark preset value; And
If higher than this benchmark preset value, this lactic acid concn value then notifies that user reduces exercise intensity, otherwise if lower than this benchmark preset value, this lactic acid concn value then notifies that user increases exercise intensity.
2. the method for training adjustment according to claim 1, it is characterized in that, this continuous percutaneous micropin sensor comprises:
One substrate;
One microneedle unit, at least comprise and be arranged in one first micropin group on this substrate and one second micropin group, this the first micropin group is as working electrode, this the second micropin group is as reference electrode, and each micropin group at least comprises a micropin, and this first micropin group comprises at least one thin slice, each thin slice at least arranges a perforation, this perforated edge is provided with a bur, and the perforation wherein on a thin slice is passed for the bur of the perforated edge of relative position on remaining thin slice, and those burs are disconnected from each other;
One signal processing unit, to be arranged on this substrate and to be electrically connected with this first and second micropins group; And
One power subsystem, supply working power gives this monitoring system.
3. the method for training adjustment according to claim 2, it is characterized in that, this the first micropin group is by one first thin slice and one second thin slice is stacked forms, this first thin slice is at least arranged one first perforation, this first perforated edge is provided with one first bur, and this second thin slice at least being arranged one second perforation, this second perforated edge is provided with one second bur, and this second bur is relative with this first bur through this first perforation of relative position on this first thin slice.
4. the method for training adjustment according to claim 2, it is characterized in that, this the first micropin group is by one first thin slice, one second thin slice and one the 3rd thin slice is stacked forms, this first thin slice is at least arranged one first perforation, this first perforated edge is provided with one first bur, this second thin slice is at least arranged one second perforation, this second perforated edge is provided with one second bur, and the 3rd thin slice at least arranges one the 3rd perforation, 3rd perforated edge is provided with one the 3rd bur, this second bur and the 3rd bur are triangular pyramidal through this first perforation on this first thin slice and this first bur.
5. the method for training adjustment according to claim 2, it is characterized in that, this the first micropin group is by one first thin slice, one second thin slice, one the 3rd thin slice and one the 4th thin slice is stacked forms, this first thin slice is at least arranged one first perforation, this first perforated edge is provided with one first bur, this second thin slice is at least arranged one second perforation, this second perforated edge is provided with one second bur, 3rd thin slice is at least arranged one the 3rd perforation, 3rd perforated edge is provided with on one the 3rd bur and the 4th thin slice and at least arranges one the 4th perforation, 4th perforated edge is provided with one the 4th bur, this second bur, 3rd bur and the 4th bur are corner taper through this first perforation on this first thin slice and this first bur.
6. the method for training adjustment according to claim 2, is characterized in that, the micropin of this first and second micropins group is formed by punching press or etch process.
7. the method for training adjustment according to claim 2, is characterized in that, the inner surface of those burs scribbles lactic acid sensing ferment.
8. the method for training adjustment according to claim 2, it is characterized in that, this continuous percutaneous micropin sensor more comprises a test-paper, be placed between this first micropin group and this substrate, this test-paper comprises a conductive layer and is positioned at the multiple test zones on this conductive layer, those test zones are coated with lactic acid sensing ferment, and align with this perforation in this first micropin group.
9. the method for training adjustment according to claim 2, it is characterized in that, the material of those burs is selected from rustless steel, nickel, nickel alloy, titanium, titanium alloy, CNT or silicon materials.
10. the method for training adjustment according to claim 2, it is characterized in that, the material of those burs is resin, and has the metal of biocompatibility in surface deposition.
The method of 11. training adjustment according to claim 1, it is characterized in that, this benchmark preset value is lactic acid threshold value, maximum lactic acid steady-state value, blood lactase acid heap initial value.
The method of 12. training adjustment according to claim 1, it is characterized in that, this benchmark preset value is the anaerobic threshold of user individual.
13. 1 kinds of lactic acid measuring equipments, is characterized in that, comprise:
One substrate;
One microneedle unit, at least comprise and be arranged in one first micropin group on this substrate and one second micropin group, this the first micropin group is as working electrode, this the second micropin group is as reference electrode, and each micropin group at least comprises a micropin, and this first micropin group comprises at least one thin slice, each thin slice at least arranges a perforation, this perforated edge is provided with a bur, and the perforation wherein on a thin slice is passed for the bur of the perforated edge of relative position on remaining thin slice, and those burs are disconnected from each other;
One signal processing unit, to be arranged on this substrate and to be electrically connected with this first and second micropins group;
One comparator, for comparing a lactic acid production measured value from this signal processing unit and a benchmark preset value; And
One power subsystem, supply working power gives this monitoring system.
14. lactic acid measuring equipments according to claim 13, is characterized in that, the micropin of this first and second micropins group is formed by punching press or etch process.
15. lactic acid measuring equipments according to claim 13, it is characterized in that, more comprise a test-paper, be placed in this test-paper between this first micropin group and this substrate to comprise a conductive layer and be positioned at the multiple test zones on this conductive layer, those test zones are coated with lactic acid sensing ferment, and align with this perforation in this first micropin group.
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