CN105441359B - 一株地衣芽孢杆菌及其应用 - Google Patents
一株地衣芽孢杆菌及其应用 Download PDFInfo
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- CN105441359B CN105441359B CN201510956636.8A CN201510956636A CN105441359B CN 105441359 B CN105441359 B CN 105441359B CN 201510956636 A CN201510956636 A CN 201510956636A CN 105441359 B CN105441359 B CN 105441359B
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- China
- Prior art keywords
- bacillus licheniformis
- denitrification
- bacterial strain
- application according
- flue gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 12
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/56—Nitrogen oxides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/40—Nitrogen compounds
- B01D2257/402—Dinitrogen oxide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/40—Nitrogen compounds
- B01D2257/404—Nitrogen oxides other than dinitrogen oxide
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02C—CAPTURE, STORAGE, SEQUESTRATION OR DISPOSAL OF GREENHOUSE GASES [GHG]
- Y02C20/00—Capture or disposal of greenhouse gases
- Y02C20/10—Capture or disposal of greenhouse gases of nitrous oxide (N2O)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
本发明公开了一株地衣芽孢杆菌及其应用,所述地衣芽孢杆菌(Bacillus licheniformis)B2,由中国典型培养物保藏中心保藏,其保藏编号是CCTCC NO:M 2015590,保藏日期为2015年10月8日。本发明提供的地衣芽孢杆菌生长温度范围广,且具有高效好氧反硝化能力,能应用于处理硝酸盐废水或含NOx的烟气。该菌株可在30~50℃下在24小时内对初始浓度为140mg/L硝酸盐氮去除率达99%以上,并且没有亚硝酸盐积累。该菌株的发现可解决温度波动对生物脱氮脱硝影响大的难题。
Description
技术领域
本发明属于环境工程、生物领域,涉及一株具有好氧反硝化性能的地衣芽孢杆菌(Bacillus licheniformis)B2菌株及其在高温环境下脱除废水中脱除水中的硝酸根与亚硝酸根的应用。
背景技术
农业生产方式发生了重大改变,农药、化肥大量使用且与日俱增。农药化肥施于农作物上,其中10%~20%附着在植物体上,其余80%~90%散落在土壤、水体、大气中。农药化肥通过地表径流或降水带入等方式进入地表水体,造成富营养化,甚至污染地下水。印染和纺织废水、炼油废水、肉类加工和饲料生产废水等都含有高浓度的氨氮和硝酸盐。另外,由于能源需求日益强劲,矿物燃料的燃烧、汽车尾气的排放量不断增加,大气中活性氮化合物含量近年来迅速增长。氮污染不仅破坏生态系统,也严重危害到人体健康。
在众多处理硝酸氮废水的研究中生物脱除法具有对环境影响程度轻、工艺简单等优点,作为一种绿色高效的处理技术而越来越被重视。而在废气脱氮治理方法中,生物过滤法作为一种低成本、高效率、无二次污染的方法,已成为大气污染控制领域热点之一。
最早提出好氧反硝化(aerobic denitrification)的是Robertson和Kuenen,他们在实验室中观察到在氧气存在的条件下发生了反硝化现象。国内外学者在好氧反硝化领域进行了广泛深入的研究,证明好氧反硝化较之传统反硝化技术存在明显优势。近年来,不断有好氧反硝化菌被分离出来,存在于副球菌属、假单胞菌属、产碱菌属、芽孢杆菌属等50多个属。然而这些报道的好氧反硝化菌大多生长于常温环境下(25~35℃),关于高温条件下(40℃以上)的好氧反硝化菌报道较少,由于目前很多工业废水都超过40℃,高温菌可直接处理高温废水,节省成本。另外,在30~50℃均能保持高效反硝化效能的菌种更鲜有报道。生物法的其中一个缺点是容易受温度波动的影响,因此,筛选分离在较广温度范围下(包括高温)具有高效性能的好氧反硝化细菌的研究具有实际意义。
发明内容
本发明之目的是提供一种在较广温度范围下(包括高温)具有高效好氧反硝化性能的菌株及其用途。
本发明的目的通过以下技术方案实现:
本发明较广温度范围下具有高效好氧反硝化性能的菌株是地衣芽孢杆菌(Bacillus licheniformis)B2,该菌株具有以下特征:
(1)菌落特征:菌落表面不光滑不透明,边缘不整齐;杆菌,菌体单个,成对或链状排列;革兰氏染色阳性;芽孢椭圆形,中生;
(2)糖、醇类发酵试验显示为发酵型,接触酶、厌氧生长、V-P测定、硝酸盐还原、明胶液化、淀粉水解、酪素水解等实验均为阳性,氧化酶、卵磷脂酶、吲哚、酪氨酸、苯丙氨酸脱氨酶等实验均为阴性,可进行反硝化作用,能利用硝态氮或亚硝态氮生长;
(3)该菌株的16S rDNA基因序列特征:其16S rDNA具有序列表所示的核苷酸序列,其16S rDNA具有序列的片段长度为1272bp,在GenBank中的登录号为No.KT777466,与GenBank数据库中已知细菌的16SrDNA进行BLAST比对,结果表明该菌株与地衣芽孢杆菌(Bacillus licheniformis)的同源性为99%,其中与菌株Bacillus licheniformisstrain AV12最相似,同源性达99%。
应用MEGA软件采用Neighbor-Joining法构建16S rDNA系统发育树,确定其进化地位,结合其形态特征和生理生化特征,该菌株最有可能是地衣芽孢杆菌属(Bacilluslicheniformis),并命名为Bacillus licheniformis B2。
所述地衣芽孢杆菌用于含氮废水的脱氮处理,或用于含NOx烟气脱硝处理。地衣芽孢杆菌B2对温度耐受程度强,在30~50℃条件下生长且具有高效的好氧反硝化能力,硝酸盐去除率均≥99%,并且没有亚硝酸盐积累。此外,地衣芽孢杆菌B2对氧气的耐受性强,在11.2mg/L溶解氧浓度下,硝酸盐去除率仍达98.7%,且没有亚硝酸盐积累。以上特性表明该菌株能应用于温度波动大、氧气含量高的实际高温废水或烟气中,将含氮污染物还原为氮气,达到脱氮的目的。
优选地,所述地衣芽孢杆菌B2用于30~50℃含氮废水的高效好氧反硝化脱氮处理。
优选地,所述地衣芽孢杆菌B2用于30~50℃烟气波动情况下的高效好氧反硝化脱硝处理。
所述地衣芽孢杆菌B2脱氮、脱硝处理的条件为:利用葡萄糖、蔗糖或丁二酸钠为碳源,控制C/N为6~15,pH为7~9,振荡转速为120~200rpm。
优选地,所述C/N为9,pH为8,振荡转速为160rpm。
与现有技术相比,本发明具有如下有益效果:
(1)本发明提供的地衣芽孢杆菌生长温度范围广,且具有高效好氧反硝化能力,能应用于处理硝酸盐废水或含NOx的烟气。
(2)该菌株可在30~50℃下在24小时内对初始浓度为140mg/L硝酸盐氮去除率达99%以上,并且没有亚硝酸盐积累。
(3)该菌株的发现可解决温度波动对生物脱氮脱硝影响大的难题。
本发明所述地衣芽孢杆菌(Bacillus licheniformis)B2,由中国典型培养物保藏中心保藏(简称CCTCC),其保藏编号是CCTCC NO:M 2015590,保藏日期为2015年10月8日,保藏地址为中国武汉大学。
附图说明
图1为Bacillus licheniformis B2的系统发育树图。
图2不同碳源下菌Bacillus licheniformis B2的反硝化性能比较图。
图3不同C/N下菌Bacillus licheniformis B2的反硝化性能比较图。
图4不同转速下菌Bacillus licheniformis B2的反硝化性能比较图。
图5不同pH下菌Bacillus licheniformis B2的反硝化性能比较图。
图6不同温度下菌Bacillus licheniformis B2的反硝化性能比较图。
图7为烟气脱硝生物过滤装置示意图,进气口1、雾化器增湿器2、温度控制器3,生物滴滤塔4、恒温水浴锅5、质量流量计6、出气口7。
图8为在真实烟气环境下培养菌Bacillus licheniformis B2的反硝化性能图。
具体实施方式
下面结合具体实施例对本发明作进一步具体详细描述,但本发明的实施方式不限于此,对于未特别注明的工艺参数,可参照常规技术进行。
实施例1:菌种的分离、纯化及鉴定
本研究向玉米叶水解液中接入不含碳源的反硝化培养基,初始温度为40℃,160rpm的振荡培养箱中。培养24h后,以体积比为10%的接种量接入90mL无菌的含反硝化培养基的玉米叶水解液中。重复上述步骤3天后发现菌液生长良好,并且脱氮率达到了85%以上。经过富集培养后的菌液通过倍比稀释法后涂布平板以分离出单菌落。将菌液取1mL至装有9mL无菌水的试管中,充分混合后得到10-1梯度的菌悬液,以此类推制备10-2~10-8不同梯度的菌悬液,为了获得可以清晰观察的菌落形态,选取10-4、10-5、10-6三个梯度的菌悬液,各取0.1mL涂布至已制备好的带有溴百里酚蓝(BTB)显色的反硝化固体培养基上,倒置放入40℃恒温恒湿培养箱中。培养2天后,可见平板中长有单菌落,由于BTB指示剂的作用,发生反硝化作用的菌落周围产生蓝色晕圈。从平板中挑取形态不同且周围有蓝色晕圈的单菌落,在无菌的反硝化固体培养基中划线后放置在40℃恒温恒湿培养箱中培养,如此重复三次得到纯菌落。
在复筛阶段将上述分离得到后的不同形态的单菌落分别接种于装有90ml的无菌反硝化培养基中,在40℃,160rpm条件下振荡培养24小时,取培养后的菌液测量OD600值以及培养液中的硝态氮浓度包括硝酸盐氮和亚硝酸盐氮,考察菌株在24h内的生长状况和硝态氮去除效果,最后筛选出一株优势菌株编号为B2,其24h内脱氮率大于95%。
将上述分离纯化得到的菌株进行生理生化鉴定及16S rDNA测序。PCR引物为通用引物16SF:5′-AGAGTTTGATCATGGCTCAG-3′(上游引物)和16SR:5′-GGTACCTTGTTACGAC TT-3′(下游引物)。PCR扩增反应条件为:94℃预变性1min,经过30个循环,56℃退火1min,72℃扩增2min,最后72℃延伸7min。得到的产物进行16S rDNA测序(上海英俊生物技术有限公司),片段长度为1272bp。将测序结果在NCBI网站上进行BLAST,结果得出B2与Bacilluslicheniformis同源性达99%,命名为Bacillus licheniformis B2(GenBank登录号为KT777466)。利用MEGA软件进行多序列比,运用Neighbor-Joining法构建系统发育树,详见图1。为了进一步确定鉴定结果的可靠性,请广东省微生物分析测试中心对该菌进行生理生化鉴定,最终确定该菌为地衣芽孢杆菌Bacillus licheniformis。
实施例2:地衣芽孢杆菌(Bacillus licheniformis)B2反硝化性能测定
1、不同碳源下Bacillus licheniformis B2的反硝化性能
考察菌株B2对不同碳源的利用情况,包括葡萄糖、蔗糖、乙酸钠、丁二酸钠、柠檬酸钠这5种碳源。具体操作步骤如下:
将10mL菌悬液接种于90mL反硝化培养基中,反硝化培养基成分如下(g/L):KNO31;Na2HPO4·7H2O 0.8;KH2PO4 1.5;MgSO4·7H2O 0.2;微量元素溶液2mL/L;pH 7.0。微量元素溶液成份(g/L):EDTA 50.0;ZnSO4 2.2;CaCl2 5.5;MnCl2·4H2O 5.06;FeSO4·7H2O 5.0;(NH4)6Mo7O2·4H2O 1.1;CuSO4·5H2O 1.57;CoCl2·6H2O 1.61。分别加入上述5种碳源,使C/N为12,于40℃、160rpm条件下振荡培养。
取0h和24h的样检测硝酸氮、亚硝酸氮、OD600,结果如图2所示,菌株对葡萄糖、蔗糖、丁二酸钠的利用率都比较高,硝酸根去除率均超过98%,但丁二酸钠为碳源时培养液中有轻微亚硝酸盐积累(1.5mg/L)。而乙酸钠作为碳源时,硝酸根去除率仅为1.09%。葡萄糖为碳源时,细菌生长量最大,OD600达1.99。这可能是由于玉米叶水解液含有大量糖类尤其是葡萄糖,B2更倾向于利用葡萄糖生长。
2、不同碳氮比下Bacillus licheniformis B2的反硝化性能
将10mL菌悬液接种于90mL反硝化培养基中,反硝化培养基中KNO3含量固定为1g/L,改变葡萄糖含量使C/N为3、6、9、12、15,其他组分如上述实验,于160rpm、40℃条件下振荡培养24h,以确定最优碳氮比。
取0h和24h的培养液检测硝酸氮、亚硝酸氮、OD600,结果如图3所示,最佳C/N为9,菌株此条件下硝酸根去除率达到100%,OD600达2.08。进一步加大葡萄糖含量并没有带来积极作用。
3、不同转速下Bacillus licheniformis B2的反硝化性能
接种10mL菌液进入90mL的反硝化液体培养基中,反硝化培养基中其他组分不变,葡萄糖为3.12g/L,调节振荡培养箱转速分别为0、60、120、160、180、200rpm,分别对应了缺氧以及氧含量逐步增加的过程,温度均为40℃,培养24h,以考察在不同氧含量情况下,B2的反硝化效果及生长状况。
测定0h和24h的硝酸氮、亚硝酸氮、OD600,结果如图4所示,OD600在160rpm下取得最大值2.05。菌株在120~200rpm条件下,反硝化率均超过99%,且没有亚硝酸盐积累,表明在高氧含量的情况,B2也能进行高效脱氮。因此,B2在实际废水或废气治理中有广泛的应用前景。
4、不同pH条件下Bacillus licheniformis B2的反硝化性能
接种10mL菌液进入90mL的反硝化液体培养基中,分别调节初始pH至6、7、8、9、10,均以160rpm的转速振荡培养24h,以考察在不同pH条件下,菌株B2的反硝化效果及生长状况。
测定0h和24h的硝酸氮、亚硝酸氮、OD600,结果如图5所示,菌株在pH为7~9条件下,反硝化率均≥99.5%,表明B2在pH波动下也能进行高效好氧反硝化。在pH为8时,菌液浓度最高。由于利用葡萄糖为碳源时,菌株会进一步分解葡萄糖导致pH的下降,因此偏碱性有利于菌株的生长。
5、不同温度Bacillus licheniformis B2的反硝化性能
接种10mL菌液进入90mL的反硝化液体培养基中,分别在30℃、35℃、40℃、45℃、50℃和55℃条件下,均以160rpm的转速振荡培养24h,以考察在不同温度下,菌株B2的反硝化效果及生长状况。
测定0h和24h的硝酸氮、亚硝酸氮、OD600,结果如图6所示,虽然菌株在30℃下菌液浓度最高,但在30~50℃的条件下,反硝化效率均达到99%及以上且没有亚硝酸盐积累,表明在上述温度范围内,菌株体内的硝酸盐还原酶和亚硝酸盐还原酶不受温度的影响,这种情况是极为罕见的。B2可在较广的温度范围内(包括高温)进行高效脱氮,这对应用于温度波动大的实际含氮废水或烟气具有深远意义。
实施例3:地衣芽孢杆菌(Bacillus licheniformis)B2的实际烟气脱硝实验
利用生物滴滤塔进行高温环境下脱除NOx的实验。生物过滤系统如图7所示,进气口1、雾化器增湿器2、温度控制器3,生物滴滤塔4、恒温水浴锅5、质量流量计6、出气口7。生物滤塔挂膜期储液池内营养组分同实施例2,考察系统在实际烟气波动的情况下运行的稳定性。
烟气流速为1L·min-1,停留时间为1min。为使B2始终占优势地位,每三天更换三分之二含10%新鲜B2的营养液。硝酸盐去除效率逐渐提高,达到80%后,逐渐降低营养液中硝酸盐的浓度并通入烟气,最终营养液中不加入硝酸盐。由于烟气波动较大,每天测定一次NOx含量,7天计算一次平均值,结果如图8所示,NOx去除率由第一周的47.5%上升至第6周的92%,然后在第7周稍有下降,为88.6%,第8周又稍有上升。另外,从第5周开始系统基本稳定运行,去除率维持在88~92%之间,说明此生物滴滤塔在有氧和烟气波动条件下能有效去除NOx。
该实例证明在实际烟气波动条件下,地衣芽胞杆菌Bacillus licheniformis B2具有高效的好氧反硝化性能,对NOx有较好的去除效果。
实施例4:地衣芽孢杆菌(Bacillus licheniformis)B2用于水耕废水脱氮
将该菌用于某温室水耕废水的脱氮处理。废水流量为155m3/d,NO3 --N325mg/L,pH6.45,水温在30℃左右,处理工艺为生物曝气滤池。
调节废水pH至8左右,将B2按0.5%的接种量添加到曝气生物池中,处理后平均NO3 --N为9.8mg/L,去除率为97.0%,除去在缩短水力停留时间的时候出现短暂的去除率降低之外,并没有发现过大的波动,并且没有亚硝酸盐积累。以投加地衣芽孢杆菌JH8(申请号为201410137313.1)做对照组,其NO3 --N去除率仅83.2%,出水亚硝酸盐浓度为0.5mg/L。由于JH8适宜温度范围为45~55℃,JH8投加至处理温度为30℃的生物曝气滤池系统中并不能提高其处理能力。相反,B2的投加明显提高了系统的脱氮性能,该菌能应用于高硝氮废水的处理。
实施例5:地衣芽孢杆菌(Bacillus licheniformis)B2用于地下水脱氮处理
将该菌用于某区域地下水的脱氮处理。水质中NO3 --N浓度为20.50mg/L,NO2 --N0.52mg/L,NH4 +-N 3.83mg/L,各项氮指标相对比较低;将该菌按0.5%的接种量添加至逆流式生物滤池中,稳定后测得地下水经处理后的NO3 --N与NO2 --N完全去除,NH4 +-N为0.07mg/L。以投加地衣芽孢杆菌JH8(申请号为201410137313.1)作对照组,其处理后水质(NO3 --N0.5mg/L,NO2 --N 0.11mg/L,NH4 +-N 0.25mg/L)。经比较可见,B2在较低浓度氨氮、硝氮以及亚硝氮的同步硝化反硝化速率要高于投加JH8的处理系统。因此,该菌对低浓度的氨氮与亚硝酸氮也能起到较好脱除效果,可以用于地下水氮污染的治理。
上述实施例为本发明较佳的实施方式,但本发明的实施方式并不受上述实施例的限制,其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化,均应为等效的置换方式,都包含在本发明的保护范围之内。
Claims (9)
1.一株地衣芽孢杆菌,其特征在于,其为地衣芽孢杆菌(Bacillus licheniformis)B2,由中国典型培养物保藏中心保藏,其保藏编号是CCTCC NO:M 2015590,保藏日期为2015年10月8日。
2.权利要求1所述地衣芽孢杆菌的应用,其特征在于,所述地衣芽孢杆菌B2用于含氮废水的脱氮处理,或用于含NOx烟气脱硝处理。
3.根据权利要求2所述的应用,其特征在于,所述地衣芽孢杆菌B2用于30~50℃含氮废水的好氧反硝化脱氮处理。
4.根据权利要求2所述的应用,其特征在于,所述地衣芽孢杆菌B2用于30~50℃烟气波动情况下的好氧反硝化脱硝处理。
5.根据权利要求2或3或4所述的应用,其特征在于,所述地衣芽孢杆菌B2脱氮、脱硝处理的条件为:利用葡萄糖、蔗糖或丁二酸钠为碳源,控制C/N为6~15,pH为7~9,振荡转速为120~200rpm。
6.根据权利要求5所述的应用,其特征在于,所述C/N为9。
7.根据权利要求5所述的应用,其特征在于,所述pH为8。
8.根据权利要求5所述的应用,其特征在于,所述振荡转速为160rpm。
9.根据权利要求7所述的应用,其特征在于,所述振荡转速为160rpm。
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