CN105435093A - Turmeric composition, turmeric gel as well as preparation method and application of turmeric gel - Google Patents
Turmeric composition, turmeric gel as well as preparation method and application of turmeric gel Download PDFInfo
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- CN105435093A CN105435093A CN201510831658.1A CN201510831658A CN105435093A CN 105435093 A CN105435093 A CN 105435093A CN 201510831658 A CN201510831658 A CN 201510831658A CN 105435093 A CN105435093 A CN 105435093A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/67—Piperaceae (Pepper family), e.g. Jamaican pepper or kava
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The invention relates to turmeric composition. The composition comprises components in parts by weight as follows: 5-20 parts of turmeric oil, 0.05-0.5 parts of pepper oil and 0.1-1 part of dementholized peppermint oil. The invention further provides turmeric gel containing the composition and a preparation of the turmeric gel. The turmeric composition and the turmeric gel can be used for relieving various painful symptoms such as inflammatory pain, muscle pain, soft tissue pain and the like. According to the composition, the turmeric oil, the pepper oil and the dementholized peppermint oil are reasonably proportioned and have a synergistic function, can realize an excellent analgesic effect, also has excellent transdermal absorption performance and is low in toxicity and safe to use; the turmeric gel is good in stability and can be stored for a long time.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to a kind of for lenitive Rhizoma Curcumae Longae compositions and the Rhizoma Curcumae Longae gel containing described compositions.
Background technology
Traditional Chinese medical theory mainly thinks the diseases such as rheumatic arthritis, rheumatoid arthritis, periarticular inflammation.Belong to the traditional Chinese medical science " arthromyodynia " category.Numbness person, close and obstructed meaning also, " stagnation of QI and blood may bring about pain " take pain as primary symptom.Whole-body muscle, joint, skin is all or part of all can suffer from, and especially with extremities joint, local skin is suffered from as many.The traditional Chinese medical science thinks that its cause of disease is all gas miscellaneous diseases that wind and cold is wet, combined into numbness.Because position, evil resistance part is different, therefore painful area is different, no matter which kind of arthromyodynia, all with " bitterly, acid, fiber crops, heavy " for main clinical characteristics.Modern medicine then thinks that it is a kind of chronic pro-inflammatory disease, with paraplasm fibroblastic in synovial fluid for feature.
Research shows, Turmeric has certain rheumatism and antarthritic effect.But effect is remarkable when being used alone Rhizoma Curcumae Longae, and orally takes and be unfavorable for giving full play to of Rhizoma Curcumae Longae therapeutic effect.
Summary of the invention
The object of the invention is the defect overcoming prior art, provide a kind of safe, effectively and the Chinese medicine composition of Transdermal absorption performance excellence.
Compositions provided by the invention, using turmeric oil as Main Ingredients and Appearance, is aided with oil of pepper and Oleum menthae; Wherein, the major function of turmeric oil is removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, can be used for alleviating rheumatism shoulder arm pain, treating swelling and pain by traumatic injury, costa sternales twinge etc., modern medicine is studied further and is shown, Rhizoma Curcumae Longae plays rheumatism and antarthritic effect by lowering the inflammatory factors such as COX-2, TNF; The major function of oil of pepper is warming spleen and stomach for dispelling cold, the therapeutic method to keep the adverse QI flowing downwards, antiinflammatory; Oleum menthae has the effect of dispelling wind-heat, refresh oneself, pain relieving; Turmeric oil, oil of pepper and Oleum menthae use jointly with rational ratio, can realize synergistic function, and oil of pepper and Oleum menthae have promotion osmosis, can promote the Transdermal absorption of active ingredient in turmeric oil, thus improve the therapeutic effect of compositions.
Specifically, the present composition comprises the composition of following weight portion: turmeric oil 5 ~ 20 parts, oil of pepper 0.05 ~ 0.5 part, Oleum menthae 0.1 ~ 1 part.
Preferably, described compositions comprises the composition of following weight portion: turmeric oil 8 ~ 12 parts, oil of pepper 0.1 ~ 0.3 part, Oleum menthae 0.4 ~ 0.6 part.
Wherein, described turmeric oil is prepared from by the method comprised the following steps: get Rhizoma Curcumae Longae medical material, soaks post-heating to boiling, in reflux, extract, collects oil reservoir, to obtain final product.Be preferably: get Rhizoma Curcumae Longae medical material, add the water soaking 10 ~ 15 hours accounting for its weight 5 ~ 6 times, be heated to boiling, reflux, extract, 30 ~ 35 hours under 0.3 ~ 0.5kg pressure, collect oil reservoir, to obtain final product.
Described oil of pepper is prepared from by the method comprised the following steps: get fresh pepper fruits, pulverizes, carries out high-pressure saturated water vapor distillation, carry out oil-water separation after condensation, collects oil reservoir, to obtain final product.
Described Oleum menthae is prepared from by the method comprised the following steps: get Herba Menthae herb, carries out high-pressure saturated water vapor distillation, and cold postpone is filtered, and collects oil reservoir, to obtain final product.Described cold temperature of putting is preferably 0 ~ 5 DEG C.
Invention further provides a kind of Rhizoma Curcumae Longae gel containing described compositions, described Rhizoma Curcumae Longae gel is the gel-type external preparation adopting outer targeting percutaneous technique to prepare.
Specifically, described Rhizoma Curcumae Longae gel pack is containing the composition of following weight portion: turmeric oil 5 ~ 20 parts, oil of pepper 0.05 ~ 0.5 part, Oleum menthae 0.1 ~ 1 part, gel-type vehicle 1 ~ 5 part, wetting agent 10 ~ 30 parts, dispersant 0.5 ~ 5 part, 50 ~ 90 parts, water.
Described gel-type vehicle be selected from carbomer, sodium alginate, sodium carboxymethyl cellulose, tragacanth, guar gum one or more; Be preferably carbomer.
Described wetting agent be selected from glycerol, propylene glycol, butanediol, sorbitol, Polyethylene Glycol one or more; Be preferably glycerol.
Described dispersant be selected from soybean phospholipid, sucrose fatty acid ester, Polysorbate, fatty glyceride one or more; Be preferably soybean phospholipid.
For the ease of long term storage, in described gel, appropriate antiseptic can also be comprised, be preferably the antiseptic of interpolation 0.1 ~ 0.5 part.Described antiseptic can select the conventional composition of this area, is preferably disodiumedetate.
As preferred version of the present invention, described Rhizoma Curcumae Longae gel comprises the composition of following weight portion: turmeric oil 8 ~ 12 parts, oil of pepper 0.1 ~ 0.3 part, Oleum menthae 0.4 ~ 0.6 part, carbomer 1.5 ~ 2.5 parts, glycerol 15 ~ 25 parts, soybean phospholipid 1 ~ 2 part, disodiumedetate 0.2 ~ 0.4 part, 65 ~ 75 parts, water.
Invention further provides the preparation method of described Rhizoma Curcumae Longae gel, comprise following concrete steps:
(1) get Rhizoma Curcumae Longae compositions and dispersant, be fully mixed to formation homogeneous phase solution at 55 ~ 65 DEG C, obtain oil phase, for subsequent use;
(2) get all the other compositions, mix homogeneously, be swelling to formation homogeneous phase solution 65 ~ 75 DEG C of insulations, obtain aqueous phase, for subsequent use;
(3) under 65 ~ 75 DEG C of conditions, while stirring described oil phase is added in aqueous phase, mix homogeneously, emulsifying, de-bubble, be cooled to room temperature, to obtain final product.
Step (3) described emulsifying is preferably carried out in high shear microemulsified tank.
The Rhizoma Curcumae Longae gel be prepared from according to the method described above, after quality inspection, fill, can put preservation in storage.Described gel preferably uses medicinal polyethylene/aluminum/polyethylene compound ointment tube packaging.In order to ensure its stability, described gel should be preserved in In Shade sealing.
The present invention protects described Rhizoma Curcumae Longae gel preparing the application in alleviating pain medicine further.Described pain comprises inflammatory pain, myalgia, soft tissue pain etc.
Specifically, described Rhizoma Curcumae Longae gel can removing blood stasis circulation of qi promoting, inducing menstruation to relieve menalgia, specifically be applicable to the symptomatic treatment of the caused inflammatory pain such as osteoarthritis, degeneration rheumatism, as the arthralgia that osteoarthritis, rheumatic arthritis, rheumatoid arthritis, acute and chronic ankylosing spondylitis, spondylarthritis, spondylalgia syndrome etc. cause; Also can be used for the mild to moderate pain alleviating various muscle, soft tissue, as scapulohumeral periarthritis, bursitis, tendinitis, tenosynovitis, myalgia, shoulder pain, lumbago and backache, and sprain, pull, dampen, equivalent damage pain of straining.
Described compound turmeric gel is external, is preferably and uses 3 ~ 4 times every day.In use, desirable gel is appropriate, and uniform application, in painful area, is gently rubbed and made gel infiltration skin.
In compositions provided by the invention, turmeric oil, oil of pepper and Oleum menthae, with rational proportioning synergism, have excellent Transdermal absorption performance, and toxicity are low, use safety while realizing excellent analgesic effect; Rhizoma Curcumae Longae gel stability containing described compositions is good, can preserve for a long time.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Present embodiments provide a kind of Rhizoma Curcumae Longae compositions, it consists of: turmeric oil 10g, oil of pepper 0.2g, Oleum menthae 0.5g.
Embodiment 2
Present embodiments provide a kind of Rhizoma Curcumae Longae compositions, it consists of: turmeric oil 12g, oil of pepper 0.3g, Oleum menthae 0.6g.
Embodiment 3
Present embodiments provide a kind of Rhizoma Curcumae Longae compositions, it consists of: turmeric oil 8g, oil of pepper 0.1g, Oleum menthae 0.4g.
Embodiment 4
Present embodiments provide a kind of Rhizoma Curcumae Longae compositions, it consists of: turmeric oil 20g, oil of pepper 0.5g, Oleum menthae 1g.
Embodiment 5
Present embodiments provide a kind of Rhizoma Curcumae Longae compositions, it consists of: turmeric oil 5g, oil of pepper 0.05g, Oleum menthae 0.1g.
Embodiment 6
Present embodiments provide a kind of Rhizoma Curcumae Longae gel, on the basis of Rhizoma Curcumae Longae compositions described in embodiment 1, add carbomer 2g further, glycerol 20g, soybean phospholipid 1.5g, disodiumedetate 0.3g, water 70g.
Embodiment 7
Present embodiments provide a kind of Rhizoma Curcumae Longae gel, on the basis of Rhizoma Curcumae Longae compositions described in embodiment 2, add carbomer 1.5g further, glycerol 15g, soybean phospholipid 1g, disodiumedetate 0.2g, water 65g.
Embodiment 8
Present embodiments provide a kind of Rhizoma Curcumae Longae gel, on the basis of Rhizoma Curcumae Longae compositions described in embodiment 3, add carbomer 2.5g further, glycerol 25g, soybean phospholipid 2g, disodiumedetate 0.4g, water 75g.
Embodiment 9
Present embodiments provide a kind of Rhizoma Curcumae Longae gel, on the basis of Rhizoma Curcumae Longae compositions described in embodiment 4, with the addition of carbomer 5g further, glycerol 30g, soybean phospholipid 5g, disodiumedetate 0.5g, water 90g.
Embodiment 10
Present embodiments provide a kind of Rhizoma Curcumae Longae gel, on the basis of Rhizoma Curcumae Longae compositions described in embodiment 5, add carbomer 2g further, glycerol 20g, soybean phospholipid 1.5g, disodiumedetate 0.3g, water 70g.
Embodiment 11
Compared with embodiment 6, difference is only: replace carbomer with sodium alginate.
Embodiment 12
Compared with embodiment 6, difference is only: replace glycerol with propylene glycol.
Embodiment 13
Compared with embodiment 6, difference is only: replace soybean phospholipid with Polysorbate T80.
Embodiment 14
The present embodiment provides the preparation method of Rhizoma Curcumae Longae gel, is specially:
(1) get Rhizoma Curcumae Longae compositions and dispersant, be fully mixed to formation homogeneous phase solution at 60 DEG C, obtain oil phase, for subsequent use;
(2) get all the other compositions, mix homogeneously, be swelling to formation homogeneous phase solution 70 DEG C of insulations, obtain aqueous phase, for subsequent use;
(3) under 70 DEG C of conditions, add in aqueous phase while stirring, mix homogeneously by described oil phase, at high shear microemulsified tank internal emulsification, de-bubble, is cooled to room temperature, to obtain final product.
Gained Rhizoma Curcumae Longae gel, after quality inspection, fill, can put preservation in storage.
Rhizoma Curcumae Longae gel described in embodiment 6 ~ 13 all can adopt above method to prepare.
Experimental example 1: stability experiment
This experimental example detects the stability of Rhizoma Curcumae Longae gel under different tests condition that embodiment 6 ~ 13 provides.Described stability do as one likes shape, discriminating, uniformity, volatile oil content four indexs judge.Concrete determination methods is:
Character: judge the color of gel, state and abnormal smells from the patient.
Differentiate: according to the regulation about effective ingredient in Rhizoma Curcumae Longae in " Chinese Pharmacopoeia " 2010 version one " active ingredient of Chinese herbs analysis handbook ", adopt the photograph thin layer chromatography that " Chinese Pharmacopoeia " 2010 editions annex VIB specify, detect the curcumin content in gel, meet States Pharmacopoeia specifications be qualified;
Uniformity: observe gel and whether keep homogeneous phase, whether layering.
Volatile oil content: the determination of volatile oil method adopting " Chinese Pharmacopoeia " 2010 editions annex XD to specify detects the content of volatile oil in Rhizoma Curcumae Longae gel.
1.1, accelerated test
Rhizoma Curcumae Longae gel pack embodiment 6 ~ 13 provided is in medicinal polyethylene/aluminum/polyethylene compound ointment tube, 30 DEG C ± 2 DEG C, under the condition of relative humidity 65% ± 5%, place 6 months, respectively at the 0th, sampling in 1,2,3,6 month, carry out every inspection to measure, testing result is as shown in table 1.
Table 1: Rhizoma Curcumae Longae gel accelerated test result
Accelerated test result shows, in 6 months, every inspection target of the Rhizoma Curcumae Longae gel that embodiment 6 ~ 8 provides has no significant change, has good stability; The Rhizoma Curcumae Longae gel stability that embodiment 9 ~ 13 provides slightly is worse than embodiment 6 ~ 8.In each embodiment, in 6 months, volatile oil content is all stabilized in the scope of 2.30 ~ 2.55ml/g.
1.2, long term test
Rhizoma Curcumae Longae gel pack embodiment 6 ~ 8 provided is in medicinal polyethylene/aluminum/polyethylene compound ointment tube, 25 DEG C ± 2 DEG C, place 24 months under relative humidity 60% ± 10% condition, respectively at the 0th, sampling in 3,6,9,12,18,24 months, detect indices.
After testing, in 24 months, the character of the Rhizoma Curcumae Longae gel that embodiment 6 ~ 8 provides, discriminating, uniformity, volatile oil content four indexs are showed no significant change, have good stability.
1.3, high wet test
Rhizoma Curcumae Longae gel embodiment 6 ~ 8 provided is placed in the airtight clean container of constant humidity, in 25 DEG C, place 10 days under RH92.5% condition, respectively at sampling in the 0th, 5,10 day, detect indices.
After testing, under conditions of high humidity, the character of the Rhizoma Curcumae Longae gel that embodiment 6 ~ 8 provides, discriminating, uniformity, volatile oil content four indexs are showed no significant change, have good stability.
1.4, strong illumination test
Rhizoma Curcumae Longae gel embodiment 6 ~ 8 provided is placed 10 days under being placed in illumination 4500Lx illuminance, respectively at sampling in the the 0th, 5,10 day, respectively at sampling in the 0th, 5,10 day, detects indices.
After testing, under strong illumination condition, the character of the Rhizoma Curcumae Longae gel that embodiment 6 ~ 8 provides, discriminating, uniformity, volatile oil content four indexs are showed no significant change, have good stability.
Experimental example 2: effect experiment
For the product that embodiment 6 provides, detect the therapeutic effect of compound recipe Fructus Evodiae gel.
In this experimental example, be divided into 5 experimental grouies, comprise:
Model group a: injecting normal saline;
Diclofenac group: use diclofenac (diclofenac diethylammonium ointment) purchased from Novartis Vaccines & Diagnostic of Switzerland;
Rhizoma Curcumae Longae gel low dose group: compared with embodiment 6, difference is only: the consumption of turmeric oil is 5g;
Dosage group in Rhizoma Curcumae Longae gel: i.e. embodiment 6, the consumption of turmeric oil is 10g;
Rhizoma Curcumae Longae gel high dose group: compared with embodiment 6, difference is only: the consumption of turmeric oil is 20g.
2.1, the effect of Rhizoma Curcumae Longae gel on adjuvant arthritis in rats secondary affection
(1) laboratory animal: SD rat, body weight (140 ± 20g), Wuhan University's Experimental Animal Center provides, the laboratory animal quality certification number: 42000600001376, use certificate number: 00120333;
(2) experimental technique: SD rat is divided into 5 groups at random, often organizes 10; Every Mus left back sufficient sole of the foot intradermal injection Freund's complete adjuvant (purchased from Wuhan Ya Fa Bioisystech Co., Ltd) 0.1ml causes inflammation; Start percutaneous drug delivery in injection adjuvant 19d, every day 1 time, each each group dosage is only 0.5g/, and successive administration 20 days, to 38d.Periodic measurement rat offside (right side) metapedes sole of the foot volume (ml), average in group (n=10); Testing result is as shown in table 2-1.
Table 2-1: the therapeutical effect of Rhizoma Curcumae Longae gel on adjuvant arthritis in rats secondary affection
From experimental result, Rhizoma Curcumae Longae gel provided by the invention has obvious therapeutical effect to adjuvant arthritis secondary affection.
2.2, Rhizoma Curcumae Longae gel is to the antiinflammatory action of mice ear model
(1) laboratory animal: Kunming mouse, body weight (20 ± 2g), Wuhan University's Experimental Animal Center provides, the laboratory animal quality certification number: 42000600001376, use certificate number: 00120332;
(2) experimental technique: Kunming mouse is divided into 5 groups at random, often organizes 10; Carry out abdominal part depilation, respectively at abdominal part depilation place topical administration 7 days, once a day, respectively organize dosage every day and be only 0.5g/, weigh every three days once; 1h after last administration, use etherization animal, before and after auris dextra, two sides each even spread dimethylbenzene 0.02ml causes inflammation, left ear with corresponding vehicle treated in contrast, after 4 hours, mice is taken off neck put to death, two ears are cut along auricle baseline, lay round auricle at the same position of left and right ear respectively with the card punch of diameter 6mm, weigh respectively, calculate the weightening finish ratio of auris dextra relative to left ear, as swelling, average in group (n=10); Testing result is as shown in table 2-2.
Table 2-2: Rhizoma Curcumae Longae gel is on impact inflammation being caused to mice ear degree
From table 2-2 result, Rhizoma Curcumae Longae gel provided by the invention causes mice ear to inflammation and has obvious mitigation.
2.3, Rhizoma Curcumae Longae gel Dichlorodiphenyl Acetate causes the analgesic activity of mice inflammatory
(1) laboratory animal: Kunming mouse, body weight (20 ± 2g), Wuhan University's Experimental Animal Center provides, the laboratory animal quality certification number: 42000600001376, use certificate number: 00120332;
(2) experimental technique: Kunming mouse is divided into 5 groups at random, often organizes 6; Carry out abdominal part depilation, respectively at abdominal part depilation place topical administration 7 days, once a day, respectively organize dosage every day and be only 0.5g/, weigh every three days once; 1h after last administration, lumbar injection 1% acetic acid (analytical pure) solution 0.2ml/ is only respectively, observes the writhing number of times in writhing situation and 30min, and calculate its writhing response analgesia suppression ratio, average in group (n=6); Testing result is as shown in table 2-3.
Table 2-3: Rhizoma Curcumae Longae gel Dichlorodiphenyl Acetate causes the analgesic activity of mice inflammatory pain
| Group | Dosage | Writhing number of times (secondary/30min) | Suppression ratio (%) |
| Model group | 0.5g | 36.67±6.74 | / |
| Diclofenac group | 0.5g | 26.01±2.83 | 29.1 |
| Rhizoma Curcumae Longae gel (low) | 0.5g | 33.33±9.46 | 9.1 |
| Rhizoma Curcumae Longae gel (in) | 0.5g | 23.17±6.71 | 36.8 |
| Rhizoma Curcumae Longae gel (height) | 0.5g | 22.50±14.42 | 38.6 |
From table 2-3 result, Rhizoma Curcumae Longae gel Dichlorodiphenyl Acetate provided by the invention causes mice inflammatory pain and has obvious inhibitory action.
Detect according to above method, the Rhizoma Curcumae Longae gel that various embodiments of the present invention provide has effective inhibitory action to arthritis secondary affection, inflammatory pain, swelling.
Experimental example 3: safety experiment
The safety of the Rhizoma Curcumae Longae gel that various embodiments of the present invention provide is detected respectively by the test of anxious poison, long term toxication, anaphylaxis experiment and skin irritation test.
3.1, anxious poison experiment
Laboratory animal: SD rat, body weight 190 ~ 210g, Wuhan University's Experimental Animal Center provides, the laboratory animal quality certification number: 42000600001376, use certificate number: 00120333; Male and female sub-cage rearing in SPF environment.
(1) intact skin experimental technique: rat back spinal column diamond wool is shaved off, every side depilation area is about 10% of body surface area; After 24 hours, confirm that the skin of tested mouse is not injured because of unhairing, and depilation regional administration 1 time, dosage be 0.5g/ only; After administration, cover with one deck preservative film, two layers of gauze, then with the altex glove cutting off fingerstall, whole back and abdominal part are fixed successively; After sticking 24h, removing tested material, and clean medicine-feeding part with 0.9% physiological sodium chloride solution.
(2) compromised skin experimental technique: rat back spinal column diamond wool is shaved off, every side depilation area is about 10% of body surface area; After 24 hours, confirm that the skin of tested mouse is injured because of unhairing, cause scratch with fine sandpaper (400#) at depilation position, after making skin occur intensive petechia, immediately scratch site administration 1 time, dosage is 0.5g/; After administration, cover with one deck preservative film, two layers of gauze, then with the altex glove cutting off fingerstall, whole back and abdominal part are fixed successively; After sticking 24h, removing tested material, and clean medicine-feeding part with 0.9% physiological sodium chloride solution.
Raise tested rat according to a conventional method, after removing medicine 1h be the 1st observing time point, afterwards in removing medicine 7h, 24h, 48h, 72h by the 7th day, Continuous Observation record 1 week, records physical signs and the sign of animal.
After testing, after the Rhizoma Curcumae Longae gel using various embodiments of the present invention to provide in 1 week, intact skin treated animal and compromised skin treated animal are all movable normal, and every physical signs and sign, without significant change, have no overt toxicity reaction and occur.As can be seen here, Rhizoma Curcumae Longae gel provided by the invention external is without skin sensibiligen effect.
3.2, long poison experiment
Laboratory animal: SD rat, body weight 190 ~ 210g, Wuhan University's Experimental Animal Center provides, the laboratory animal quality certification number: 42000600001376, use certificate number: 00120333; Male and female sub-cage rearing in SPF environment.
Experimental technique: rat back spinal column diamond wool is shaved off, every side depilation area is about 10% of body surface area; After 24 hours, confirm that the skin of tested mouse is not injured because of unhairing, and depilation regional administration 1 time, dosage be 0.5g/ only; After administration, cover with one deck preservative film, two layers of gauze, then with the altex glove cutting off fingerstall, whole back and abdominal part are fixed successively; After sticking 24h, removing tested material, and clean up with normal saline, then with 0.5g/ dosed administration only, successive administration 30 days; Continue to raise two weeks after drug withdrawal and carry out convalescent period observation.Within administration phase and convalescent period, the physical signs of every day entry animal and sign.
After testing, after the Rhizoma Curcumae Longae gel using various embodiments of the present invention to provide, tested rat every physical signs and sign within administration phase and convalescent period, without significant change, have no overt toxicity reaction and occur.As can be seen here, Rhizoma Curcumae Longae gel provided by the invention external is without skin chronic toxic effect.
3.3, anaphylaxis experiment
Using healthy albino guinea-pig as animal subject; Shaved off by the hair of guinea pig back spinal column both sides, every side depilation area is about 10% of body surface area; After 24 hours, confirm that skin is not injured because of unhairing, and Rhizoma Curcumae Longae gel is applied in priming dose on the skin of the epilating area in left side; After administration, cover with one deck preservative film, two layers of gauze, then with the altex glove cutting off fingerstall, whole back and abdominal part are fixed successively; After sticking 6h, removing tested material, and clean up with normal saline; Again in the 7th day and the 14th day respectively in the same way and dosed administration; After last sensitization contact the 14th day, by the Rhizoma Curcumae Longae gel uniform application of booster dose on depilation district, right side skin, after 6 hours, removing tested material, and clean up with normal saline, observe Cavia porcellus depilation district skin immediately, and after 24h, 48h, 72h, repeatedly observe Cavia porcellus depilation district skin respectively.
After testing, after the Rhizoma Curcumae Longae gel using various embodiments of the present invention to provide, all there is not erythema, edema or other abnormal response in the skin of tested Cavia porcellus, as can be seen here, Rhizoma Curcumae Longae gel provided by the invention external is without skin allergy.
3.4, skin irritation test
Using healthy new zealand white rabbit as animal subject; Shaved off by the hair of spinal column both sides, white rabbit back, every side depilation area is about 10% of body surface area; After 24 hours, confirmation skin is injured because of unhairing, adopts No. 16 syringe needles after sterilization, and the skin after losing hair or feathers and sterilize in both sides draws " # " font, scratching epidermis, do not hinder corium, slight oozing of blood is degree, smears Rhizoma Curcumae Longae gel immediately at damaged skin position; After administration, cover with one deck preservative film, two layers of gauze, then with the altex glove cutting off fingerstall, whole back and abdominal part are fixed successively; After sticking 6h, removing tested material, and clean up with normal saline; Successive administration 7 days.After last administration 30 ~ 60 minutes, 24,48 and 72 hours perusal coating parts, with or without the situation such as erythema and edema.
After testing, after the Rhizoma Curcumae Longae gel using various embodiments of the present invention to provide, all there is not erythema, edema or other abnormal response in the skin of tested white rabbit, as can be seen here, Rhizoma Curcumae Longae gel provided by the invention external is without skin irritation.
Although above with general explanation, detailed description of the invention and test, the present invention is described in detail, and on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a Rhizoma Curcumae Longae compositions, is characterized in that, comprises the composition of following weight portion: turmeric oil 5 ~ 20 parts, oil of pepper 0.05 ~ 0.5 part, Oleum menthae 0.1 ~ 1 part.
2. Rhizoma Curcumae Longae compositions according to claim 1, is characterized in that, comprises the composition of following weight portion: turmeric oil 8 ~ 12 parts, oil of pepper 0.1 ~ 0.3 part, Oleum menthae 0.4 ~ 0.6 part.
3. Rhizoma Curcumae Longae compositions according to claim 1 and 2, is characterized in that, described turmeric oil is prepared from by the method comprised the following steps: get Rhizoma Curcumae Longae medical material, soaks post-heating to boiling, in reflux, extract, collects oil reservoir, to obtain final product;
And/or described oil of pepper is prepared from by the method comprised the following steps: get fresh pepper fruits, pulverizes, carry out high-pressure saturated water vapor distillation, after condensation, carry out oil-water separation, collect oil reservoir, to obtain final product;
And/or described Oleum menthae is prepared from by the method comprised the following steps: get Herba Menthae herb, carry out high-pressure saturated water vapor distillation, cold postpone is filtered, and collects oil reservoir, to obtain final product.
4. a Rhizoma Curcumae Longae gel, is characterized in that, comprises the composition of following weight portion: turmeric oil 5 ~ 20 parts, oil of pepper 0.05 ~ 0.5 part, Oleum menthae 0.1 ~ 1 part, gel-type vehicle 1 ~ 5 part, wetting agent 10 ~ 30 parts, dispersant 0.5 ~ 5 part, 50 ~ 90 parts, water.
5. Rhizoma Curcumae Longae gel according to claim 4, is characterized in that, described gel-type vehicle be selected from carbomer, sodium alginate, sodium carboxymethyl cellulose, tragacanth, guar gum one or more;
And/or, described wetting agent be selected from glycerol, propylene glycol, butanediol, sorbitol, Polyethylene Glycol one or more;
And/or, described dispersant be selected from soybean phospholipid, sucrose fatty acid ester, Polysorbate T80, fatty glyceride one or more.
6. the Rhizoma Curcumae Longae gel according to claim 4 or 5, is characterized in that, also comprises antiseptic 0.1 ~ 0.5 part.
7. Rhizoma Curcumae Longae gel according to claim 4, it is characterized in that, comprise the composition of following weight portion: turmeric oil 8 ~ 12 parts, oil of pepper 0.1 ~ 0.3 part, Oleum menthae 0.4 ~ 0.6 part, carbomer 1.5 ~ 2.5 parts, glycerol 15 ~ 25 parts, soybean phospholipid 1 ~ 2 part, disodiumedetate 0.2 ~ 0.4 part, 65 ~ 75 parts, water.
8. the preparation method of Rhizoma Curcumae Longae gel described in claim 4 ~ 7 any one, is characterized in that, comprise the following steps:
(1) get Rhizoma Curcumae Longae compositions and dispersant, be fully mixed to formation homogeneous phase solution at 55 ~ 65 DEG C, obtain oil phase, for subsequent use;
(2) get all the other compositions, mix homogeneously, be swelling to formation homogeneous phase solution 65 ~ 75 DEG C of insulations, obtain aqueous phase, for subsequent use;
(3) under 65 ~ 75 DEG C of conditions, while stirring described oil phase is added in aqueous phase, mix homogeneously, emulsifying, de-bubble, be cooled to room temperature, to obtain final product.
9. the application in alleviating pain medicine prepared by Rhizoma Curcumae Longae gel described in Rhizoma Curcumae Longae compositions described in claims 1 to 3 any one or claim 4 ~ 7 any one.
10. application according to claim 9, is characterized in that, described pain is inflammatory pain, myalgia or soft tissue pain.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125733A (en) * | 2018-10-30 | 2019-01-04 | 成都先手生物科技有限公司 | A kind of composition for the treatment of of arthritis and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269201A (en) * | 2007-12-29 | 2008-09-24 | 天津市中宝制药有限公司 | Chinese medicine compound percutaneous absorption patch for treating menorrhalgia and preparation method thereof |
-
2015
- 2015-11-25 CN CN201510831658.1A patent/CN105435093A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101269201A (en) * | 2007-12-29 | 2008-09-24 | 天津市中宝制药有限公司 | Chinese medicine compound percutaneous absorption patch for treating menorrhalgia and preparation method thereof |
Non-Patent Citations (8)
| Title |
|---|
| 刘红等: "胡椒根油树脂化学成分的研究", 《中国农学通报》 * |
| 吴斌: "姜黄油的提取、抑菌活性及姜黄色素的纯化研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 孙建宁: "《中药药理学》", 31 January 2014, 中国中医药出版社 * |
| 张仲源等: "中药促进透皮吸收剂薄荷醇的多效性", 《中医外治杂志》 * |
| 沈梅芳等: "薄荷化学成分与药理作用研究新进展", 《中华中医药学刊》 * |
| 艾铁民: "《中国药用植物志 第12卷》", 31 January 2013, 北京大学医学出版社 * |
| 邢旭等: "胡椒油的研究进展", 《农产品加工业》 * |
| 陈智坤等: "薄荷属植物挥发性成分及药理作用研究进展", 《天然产物研究与开发》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109125733A (en) * | 2018-10-30 | 2019-01-04 | 成都先手生物科技有限公司 | A kind of composition for the treatment of of arthritis and its application |
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