CN105431536B - 用于提取和储存核酸的方法和组合物 - Google Patents

用于提取和储存核酸的方法和组合物 Download PDF

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Publication number
CN105431536B
CN105431536B CN201480045324.5A CN201480045324A CN105431536B CN 105431536 B CN105431536 B CN 105431536B CN 201480045324 A CN201480045324 A CN 201480045324A CN 105431536 B CN105431536 B CN 105431536B
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China
Prior art keywords
matrix
acid
rna
nucleic acids
sample
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Chinese (zh)
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CN105431536A (zh
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E.L.克瓦姆
B.李
B.C.贝尔斯
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GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
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General Electric Co
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Priority claimed from US13/968,497 external-priority patent/US9040679B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
CN201480045324.5A 2013-08-16 2014-08-14 用于提取和储存核酸的方法和组合物 Active CN105431536B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/968,497 US9040679B2 (en) 2012-04-30 2013-08-16 Methods and compositions for extraction and storage of nucleic acids
US13/968497 2013-08-16
PCT/EP2014/067453 WO2015022410A1 (en) 2013-08-16 2014-08-14 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CN105431536A CN105431536A (zh) 2016-03-23
CN105431536B true CN105431536B (zh) 2021-01-01

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CN201480045324.5A Active CN105431536B (zh) 2013-08-16 2014-08-14 用于提取和储存核酸的方法和组合物

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EP (1) EP3033421B1 (enExample)
JP (1) JP6704621B2 (enExample)
KR (1) KR102214211B1 (enExample)
CN (1) CN105431536B (enExample)
AU (1) AU2014307875B2 (enExample)
CA (1) CA2921286C (enExample)
ES (1) ES2777220T3 (enExample)
IL (1) IL244033B (enExample)
MX (1) MX371517B (enExample)
SG (1) SG11201601114PA (enExample)
WO (1) WO2015022410A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107430114B (zh) 2015-03-31 2020-07-24 豪夫迈·罗氏有限公司 血浆分离卡
ES2912965T3 (es) * 2015-09-09 2022-05-30 Drawbridge Health Inc Dispositivos para la recopilación, estabilización y conservación de muestras
EP3528950A4 (en) * 2016-10-24 2020-05-27 Biomatrica, INC. STABILIZATION OF NUCLEIC ACIDS ON PAPER
CN106754881A (zh) * 2016-12-26 2017-05-31 广州和实生物技术有限公司 一种血液病毒rna保护剂及其制备方法与应用
CN210383905U (zh) 2017-01-10 2020-04-24 集联健康有限公司 一种用于从受试者收集流体样品的装置以及运输套筒
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
WO2023023076A1 (en) * 2021-08-16 2023-02-23 Bruce Johnson Sample encapsulation system
CN117467657A (zh) * 2022-07-22 2024-01-30 友康厚德生物科技(北京)有限公司 一种裂解组合物

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE96915910T1 (de) * 1995-06-07 2004-04-22 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
KR100738086B1 (ko) * 2005-12-21 2007-07-12 삼성전자주식회사 금속 산화물이 증착된 고체 지지체를 이용한 핵산 정제방법 및 장치
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
CN102177237B (zh) * 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
WO2010132508A2 (en) * 2009-05-11 2010-11-18 Biomatrica, Inc. Compositions and methods for biological sample storage
EA022842B1 (ru) * 2010-01-07 2016-03-31 Бигтек Прайвит Лимитед Способ выделения нуклеиновой кислоты и набор для этого
WO2012018638A2 (en) * 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CN103068979B (zh) * 2010-08-18 2015-03-11 东丽株式会社 Rna提取用溶液
US9044738B2 (en) * 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids

Also Published As

Publication number Publication date
EP3033421B1 (en) 2020-02-12
CA2921286A1 (en) 2015-02-19
JP6704621B2 (ja) 2020-06-03
CA2921286C (en) 2022-11-22
IL244033A0 (en) 2016-04-21
CN105431536A (zh) 2016-03-23
SG11201601114PA (en) 2016-03-30
ES2777220T3 (es) 2020-08-04
EP3033421A1 (en) 2016-06-22
KR102214211B1 (ko) 2021-02-10
MX371517B (es) 2020-01-31
IL244033B (en) 2020-07-30
JP2016527897A (ja) 2016-09-15
AU2014307875B2 (en) 2020-07-02
KR20160034947A (ko) 2016-03-30
MX2016001843A (es) 2016-05-16
WO2015022410A1 (en) 2015-02-19
AU2014307875A1 (en) 2016-01-28

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Effective date of registration: 20210825

Address after: England

Patentee after: Global life science solutions UK Ltd.

Address before: Buckinghamshire

Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20210825

Address after: Buckinghamshire

Patentee after: GE HEALTHCARE UK Ltd.

Address before: New York State, USA

Patentee before: General Electric Co.