CN105410024B - Compound seed soaking agent of Trifolium repense and preparation method thereof, application method and application - Google Patents
Compound seed soaking agent of Trifolium repense and preparation method thereof, application method and application Download PDFInfo
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- CN105410024B CN105410024B CN201510808563.8A CN201510808563A CN105410024B CN 105410024 B CN105410024 B CN 105410024B CN 201510808563 A CN201510808563 A CN 201510808563A CN 105410024 B CN105410024 B CN 105410024B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses compound seed soaking agent of Trifolium repense and preparation method thereof, application method and applications, belong to Trifolium repense seed soaking agent field.The compound seed soaking agent of Trifolium repense disclosed in this invention includes mainly gibberellin, sodium lignin sulfonate and vitamin K3.The invention also discloses the preparation method of the compound seed soaking agent of the Trifolium repense, application method and its applications.The present invention is it has furthermore been found that by gibberellin, sodium lignin sulfonate and vitamin K3There is the effect of extremely significant synergistic after being compounded with dodder extract, the deficiency of single component can more effectively be eliminated, Trifolium repense seed is handled with the seed soaking agent after compounding, the seed sprouting period significantly shortens, seedling after sprouting is sturdy, well developed root system, and the anti-adversity including drought resistance is significantly enhanced.
Description
Technical field
The present invention relates to being grown with various plants for seed soaking agent more particularly to a kind of suitable Trifolium repense (Leguminosae)
Conditioning agent and the compound seed soaking agent that dodder extract is active ingredient, the invention further relates to the compound seed soaking agents to shorten
Trifolium repense growing-seedling period, raising emergence rate, promoting root growth promote the application of strong or raising anti-adversity etc., belong to Trifolium repense seed soaking agent
Field.
Background technology
Trifolium repense is pulse family Trifolium perennial plant, is distributed widely in temperate zone and subtropical zone high altitude localities.China
There is a wild distribution on the ground such as Yunnan, Guizhou, Sichuan, Hunan, Hubei, Xinjiang, and there is commerial growing in each province on the south the Changjiang river.Trifolium repense
It is used as pasture growing always in China, in recent years since gardening and afforestation is by pay attention to day by day, Trifolium repense is in afforestation and water
Play the role of in terms of native holding more and more.Because its breeding is easy and extensive management, there is very strong competitiveness with weeds, thus
It is widely applied in the soil and slope protections greenings such as airport, highway, river dike loke shore, mine reparation, plays good ground
Cover and afforest and beautify effect.
Trifolium repense seed is tiny, and after planting seedling stage and the slow-growing of growth period, duration in seedling stage are long, and weeds emerge
It is early, growth is fast, to space to occupy ability strong, influence the normal growth and development of Trifolium repense, especially in Trifolium repense seed-breeding field, often
The appearance of some weeds of Chang Yinwei so that the more pure Trifolium repense seed of production becomes difficult.Meanwhile Trifolium repense root system is not sent out
It reaches, it is very sensitive to arid and salt stress.Trifolium repense needs to shorten growing-seedling period in seedling stage, improves resistance, enhancing and weeds
Competitive advantage.
To solve the above-mentioned problems, other than field management measure, common method is seed pelleting, seed dressing and seed soaking.
Trifolium repense seed is handled using seed soaking agent, simple production process, using easy, treating capacity is big can not only allow seed to fully absorb
The effect that various active ingredients, also vernalization are early sent out.
CN104012534A discloses a kind of Trifolium repense drought resisting conditioning agent, and involved plant growth regulator is salicylic acid
And brassin lactones, but the effect that the conditioning agent promotees salt tolerance that is strong, increasing Trifolium repense etc. in promoting root growth is poor, needs to be changed
Into.CN101473722B discloses Trifolium repense seed-pelleting coating method, although pest and disease damage can be effectively reduced, is carrying
There is no effect substantially in terms of its high drought resistance.
In order to overcome many defects present in existing Trifolium repense seed soaking agent, it would be highly desirable to need development one kind that can significantly contract
Short growing-seedling period effectively improves emergence rate or improves the Trifolium repense seed soaking agent of its anti-adversity.
Invention content
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide one kind to effectively shorten nursery
Period improves emergence rate, increases salt tolerance or improves the compound seed soaking agent of Trifolium repense of drought resistance.
The technical problem to be solved by the present invention is to what is be achieved through the following technical solutions：
A kind of compound seed soaking agent of Trifolium repense, by following at being grouped as：Gibberellin, sodium lignin sulfonate, vitamin K3With
Ethyl alcohol.
Preferably, by mass percentage, the dosage of each ingredient is：Gibberellin 0.5%-1.5%, sodium lignin sulfonate
1.2%-1.6%, vitamin K30.4%-0.6%, surplus are ethyl alcohol；
It is furthermore preferred that the dosage of each ingredient is：Gibberellin 1%, sodium lignin sulfonate 1.4%, vitamin K30.5%.
Gibberellin is a kind of plant hormone that can promote plant growth, have gibberellane ring structure, can promote the life of stem, leaf
It is long, but gibberellin is administered alone, plant is susceptible to excessive growth phenomenon.
Sodium lignin sulfonate is a kind of anionic surfactant, is had to heavy metal, especially copper, iron, stannous ion
Preferable sequestering power is effective chelating agent.
Higher plant is vitamin autotrophic type, but seed period and early growth period can not synthesize enough vitamin.
External source vitamin can enhance the quality of the vigor of seed, the growth of promotion seedling root, increase root and cauline leaf, improve photosynthetic effect
Rate significantly improves total absorption area of root system and enlivens absorption area.
The present invention is by gibberellin and methyl α-naphthyl acetate, calcium lignosulfonate, sodium lignin sulfonate, vitamin C, vitamin K3Deng tool
Have hestening rooting germinate ingredient it is compatible, it is expected that screening obtain one kind can not only effectively shorten growing-seedling period, improve emergence rate,
The compound seed soaking agent of Trifolium repense of anti-adversity can also be effectively improved.The present invention is had found by screening test, by gibberellin and wood
Quality sodium sulfonate, vitamin K3It is compounded, the deficiency using single component can be effectively eliminated, gibberellin makees the promotion of height of seedling
With not failing, sodium lignin sulfonate and vitamin K3Total absorption area of root system is significantly improved again and enlivens absorption area,
Root activity accordingly is improved, three's compatibility significantly enhances the resistance of Trifolium repense together, and there is extremely significant collaboration to increase
Imitate effect.
Influence between plant growth regulator is extremely complex, it is understood that there may be a variety of influences such as synergy, antagonism, it can so that having
There can be the case where binary built is better than ternary built.In addition, from the aspect of cost and preparation processing, medicament kind is reduced
With obvious advantage；The effect of ternary built is made further in consideration of it, the present invention further designs binary built experiment
Inspection.Test result is as it can be seen that gibberellin, sodium lignin sulfonate and vitamin K3The significant effect for carrying out ternary built is better than it
Its binary built scheme (difference reaches the pole level of signifiance)；Composite score is as it can be seen that gibberellin, sodium lignin sulfonate and Wei Sheng
Plain K3Compounding effect be intended to be substantially better than other compound schemes, confirmation is by gibberellin and sodium lignin sulfonate and vitamin K3
Carrying out compounding has effects that significant synergy.
Determining Trifolium repense seed soaking agent by gibberellin, sodium lignin sulfonate and vitamin K3It forms with synergy
On the basis of, the present invention is it has furthermore been found that gibberellin, sodium lignin sulfonate and vitamin K3Different amounts proportioning, for seed soaking
The influence of effect also highly significant.The present invention had found by a large amount of screening test, three kinds of ingredients according to following amount ranges into
Row compatibility has preferable synergy：
By mass percentage, gibberellin 0.5%-1.5%, sodium lignin sulfonate 1.2%-1.6%, vitamin
K30.4%-0.6%, surplus are ethyl alcohol；
Wherein, three kinds of ingredients carry out compatibility according to following dosage has best synergy：
Gibberellin 1%, sodium lignin sulfonate 1.4%, vitamin K30.5%, surplus is ethyl alcohol.
Invention further provides a kind of method preparing the Trifolium repense seed soaking agent, this method includes：By gibberellin,
Sodium lignin sulfonate and vitamin K3Be added in ethyl alcohol, stir evenly to get.
In order to reach more preferably technique effect, the ethyl alcohol is preferably the ethyl alcohol of content >=95%.
The present invention still further provides the compound seed soaking agent of the Trifolium repense and is shortening Trifolium repense growing-seedling period, improving it
Application in seedling rate, increase anti-adversity (including salt tolerance and drought resistance) etc., including：By the compound seed soaking of the Trifolium repense
Agent is diluted with water, the Trifolium repense seed soaking agent after being diluted；By Trifolium repense seed dilute after the compound seed soaking agent of Trifolium repense into
Row seed soaking.
Wherein, 500-1500 times, preferably 800-1200 times is diluted with water in the compound seed soaking agent of the Trifolium repense, more preferably
It is 1000 times；
According to mass ratio meter, Trifolium repense seed and the ratio of the compound seed soaking agent liquid of Trifolium repense after dilution are preferably 1:3；Institute
The immersion stated is preferably soak seed at a temperature of 20 DEG C -30 DEG C 1-8 hours, more preferably 6 hours.
The present invention is found surprisingly that very much：By gibberellin, sodium lignin sulfonate, vitamin K3With Semen Cuscutae (Cuscuta
Chinensis Lam) there is the effect of extremely significant synergistic after leaching liquor is compatible, it can more effectively eliminate using single
The deficiency of ingredient handles Trifolium repense seed with it, and the seed sprouting period significantly shortens, and the seedling after sprouting is sturdy, well developed root system,
Resistance including drought resistance is significantly enhanced.
Semen Cuscutae is the malignant weed for seeking parasitic life, the cauline leaf of host plant is wound with very thin vines, by haustorium
Nutriment is drawn out of plant, causes host plant growth weak or even whole strain is dead.But the mature seed of Semen Cuscutae
Contain the compounds such as flavonoids, polysaccharide, alkaloids, terpene, steroid, volatile oil and lignin.In addition, also containing big
The trace elements such as the glucosides of amount, 16 kinds of amino acid and calcium, magnesium, iron, manganese, copper, zinc, have higher medical value.
In order to screen gibberellin, sodium lignin sulfonate, vitamin K3It is matched with the optimum amount of dodder extract, this hair
The bright consumption proportion to four kinds of constituents screens, and more different dosages shortens growing-seedling period for Trifolium repense, improves
The influence of emergence rate, raising drought resistance, increase salt tolerance etc.；Test result shows that the preferable compound scheme of effect is " red
Mycin 0.5%-1.5%+ sodium lignin sulfonate 1.2%-1.6%+ vitamin Ks30.4%-0.6%, with dodder extract (Tu
The sub- stoste of silk dilutes 3 times) 1000 times of dilution ", shortening growing-seedling period using the consumption proportion and improving resistance and other effects side
Face will be significantly better than other consumption proportions (difference reaches the level of signifiance), wherein " gibberellin 1%+ sodium lignin sulfonates 1.4%+
Vitamin K30.5%, with 1000 times of dodder extract (Semen Cuscutae stoste dilute 3 times) dilution " compounding effect be all multiple
Other consumption proportions best with effect in scheme, being significantly better than in the compound scheme (difference reaches the pole level of signifiance).
Therefore, it the present invention also provides the compound seed soaking agent of another Trifolium repense, is made of ingredient I and ingredient II；According to
Mass ratio meter, ingredient I:Ingredient II=1:500-1500；Preferably, ingredient I:Ingredient II=1:750-1250；Most preferably, at
Divide I:Ingredient II=1:1000.
The ingredient I is by gibberellin, sodium lignin sulfonate, vitamin K3It is formed with ethyl alcohol；By mass percentage, ingredient
The dosage of each ingredient is in I：Gibberellin 0.5%-1.5%, sodium lignin sulfonate 1.2%-1.6%, vitamin K30.4%-
0.6%, surplus is ethyl alcohol；Preferably, the dosage of each ingredient is：Gibberellin 1%, sodium lignin sulfonate 1.4%, vitamin
K30.5%；
The ingredient II is dodder extract.
The dodder extract is obtained after Semen Cuscutae stoste is diluted with water, it is preferred that the Semen Cuscutae leaching
Extract is obtained after Semen Cuscutae stoste is diluted with water 1-5 times；It is furthermore preferred that the dodder extract is by Semen Cuscutae original
Liquid obtains after being diluted with water 2-4 times；Most preferably, the dodder extract is after Semen Cuscutae stoste is diluted with water 3 times
It obtains.
As a preferred solution, the Semen Cuscutae stoste is prepared in accordance with the following methods：
(1) it is decocted again after Semen Cuscutae mature seed being soaked in water；Decoction liquor is filtered, supernatant is taken, obtains filtrate 1；Filter
Slag is decocted with water again, and decoction liquor is filtered, and takes supernatant, obtains filtrate 2；(2) filtrate 1 and filtrate 2 are merged, concentrate to get；
Preferably, it is soaked in water after cleaning Semen Cuscutae mature seed in step (1) after 30min, boils rear slow boiling by intense fire
30min is boiled again, is filtered；Wherein, according to g:Ml is counted, and the ratio of Semen Cuscutae mature seed and water is 0.5-2:1-8, more preferably 1:
2。
In step (1), according to g:Ml is counted, and the ratio of filter residue and water is preferably 0.5-2:1-8, more preferably 1:2；
It is concentrated again after carrying out 80-90 DEG C of water-bath after merging filtrate 1 and filtrate 2 in step (2).
The present invention provides a kind of methods preparing the compound seed soaking agent of the Trifolium repense, including：Ingredient I and ingredient II are mixed
Close uniformly to get.
Correspondingly, the present invention provides the compound seed soaking agents of the Trifolium repense to shorten Trifolium repense growing-seedling period, improve its emergence
Rate increases salt tolerance or improves the application in drought resistance aspect；The application includes：By the Trifolium repense seed Trifolium repense
Compound seed soaking agent is soaked seed；Wherein, according to mass ratio meter, the ratio of Trifolium repense seed and the compound seed soaking agent liquid of Trifolium repense is excellent
It is selected as 1:3；The immersion is preferably soak seed at a temperature of 20 DEG C -30 DEG C 1-8 hours, more preferably 6 hours.
The compound seed soaking agent of Trifolium repense of the present invention is by gibberellin and sodium lignin sulfonate, vitamin K3It is compounded, then is coordinated
Dodder extract uses, and can effectively eliminate the deficiency using single medicament, and four compatibilities have extremely significant collaboration together
Synergy effect, after handling Trifolium repense seed with it, compared with the control, germinating energy improves 50%-70%, germination percentage improves 15%-
25%, height of seedling increases 40%-50%, root long increases 40%-50%；Drought resistance enhances 1-2 times, 1-2 times of salt tolerance enhancing, finally
Achieve the purpose that shorten growing-seedling period, improve germination percentage, enhancing resistance.
Specific implementation mode
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.It should be understood that described, examples are merely exemplary, does not constitute any restrictions to the scope of the present invention.This field
Technical staff should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and
Form is modified or is replaced, but these modifications or substitutions each fall within protection scope of the present invention.
The preparation of 1 Semen Cuscutae of Preparative Example (Cuscuta chinensis Lam) leaching liquor
It weighs after Semen Cuscutae mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersion 30min is added,
It boils rear slow boiling by intense fire and boils 30min again, filtered through gauze stays supernatant, and filter residue adds 200ml distilled water equally to handle 1 time again, after filtering
Residue is abandoned, 2 filtrates 80 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and Semen Cuscutae is made
Extract stoste；Semen Cuscutae extraction stoste is diluted with water 3 times, dodder extract is made.
The preparation of 2 Semen Cuscutae of Preparative Example (Cuscuta chinensis Lam) leaching liquor
It weighs after Semen Cuscutae mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersion 30min is added,
It boils rear slow boiling by intense fire and boils 15min again, filtered through gauze stays supernatant, and filter residue adds 200ml distilled water equally to handle 1 time again, after filtering
Residue is abandoned, 2 filtrates 80 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and Semen Cuscutae is made
Extract stoste；Semen Cuscutae extraction stoste water proof is diluted 4 times, dodder extract is made.
The preparation of 3 Semen Cuscutae of Preparative Example (Cuscuta chinensis Lam) leaching liquor
It weighs after Semen Cuscutae mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersion 30min is added,
It boils rear slow boiling by intense fire and boils 30min again, filtered through gauze stays supernatant, and filter residue adds 200ml distilled water equally to handle 1 time again, after filtering
Residue is abandoned, 2 filtrates 90 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and Semen Cuscutae is made
Extract stoste；Semen Cuscutae extraction stoste is diluted with water 5 times, dodder extract is made.
The preparation of 4 Cuscuta australis of Preparative Example (Cuscuta australis R.Br) leaching liquor
It weighs after Cuscuta australis mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersions is added
30min boils rear slow boiling by intense fire and boils 30min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again,
Residue is abandoned after filtering, 2 filtrates 90 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and are made
Cuscuta australis extracts stoste；Cuscuta australis extraction stoste is diluted with water 3 times, Cuscuta australis leaching liquor is made.
The preparation of 5 Cuscuta australis of Preparative Example (Cuscuta australis R.Br) leaching liquor
It weighs after Cuscuta australis mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersions is added
30min boils rear slow boiling by intense fire and boils 15min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again,
Residue is abandoned after filtering, 2 filtrates 80 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and are made
Cuscuta australis extracts stoste；Cuscuta australis extraction stoste is diluted with water 4 times, Cuscuta australis leaching liquor is made.
The preparation of 6 Cuscuta australis of Preparative Example (Cuscuta australis R.Br) leaching liquor
It weighs after Cuscuta australis mature seed 100g cleans 3 times with appropriate distilled water, 200ml distilled water immersions is added
30min boils rear slow boiling by intense fire and boils 30min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again,
Residue is abandoned after filtering, 2 filtrates 90 DEG C of water-baths of progress of merging are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and are made
Cuscuta australis extracts stoste；Cuscuta australis extraction stoste is diluted 5 times, Cuscuta australis leaching liquor is made.
The preparation of 7 argy wormwood leaching liquor of Preparative Example
The argy wormwood cauline leaf 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, after boiling by intense fire
Slow boiling boils 30min again, and filtered through gauze is stayed supernatant, filter residue to add 200ml distilled water equally to handle 1 time again, residue is abandoned after filtering, merged
2 filtrates 90 DEG C of water-baths of progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and argy wormwood extraction stoste are made；By institute
Argy wormwood extraction stoste is diluted with water 3 times argy wormwood leaching liquor is made.
The preparation of 8 argy wormwood leaching liquor of Preparative Example
The argy wormwood cauline leaf 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, after boiling by intense fire
Slow boiling boils 15min again, and filtered through gauze is stayed supernatant, filter residue to add 200ml distilled water equally to handle 1 time again, residue is abandoned after filtering, merged
2 filtrates 80 DEG C of water-baths of progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and argy wormwood extraction stoste are made；By institute
Argy wormwood extraction stoste is diluted with water 4 times argy wormwood leaching liquor is made.
The preparation of 9 argy wormwood leaching liquor of Preparative Example
The argy wormwood cauline leaf 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, after boiling by intense fire
Slow boiling boils 30min again, and filtered through gauze is stayed supernatant, filter residue to add 200ml distilled water equally to handle 1 time again, residue is abandoned after filtering, merged
2 filtrates 90 DEG C of water-baths of progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and argy wormwood extraction stoste are made；By institute
Argy wormwood extraction stoste is diluted with water 5 times argy wormwood leaching liquor is made.
The preparation of 10 granatum leaching liquor of Preparative Example
The granatum 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, are boiled by intense fire hereinafter
Fire boils 30min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again, residue is abandoned after filtering, merges 2
90 DEG C of water-baths of secondary filtrate progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and granatum extraction stoste are made；By institute
Granatum extraction stoste is diluted with water 3 times granatum leaching liquor is made.
The preparation of 11 granatum leaching liquor of Preparative Example
The granatum 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, are boiled by intense fire hereinafter
Fire boils 15min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again, residue is abandoned after filtering, merges 2
80 DEG C of water-baths of secondary filtrate progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and granatum extraction stoste are made；By institute
It obtains 4 times of granatum extraction stoste water proof dilution and granatum leaching liquor is made.
The preparation of 12 granatum leaching liquor of Preparative Example
The granatum 100g addition 200ml distilled water immersion 30min that drying and crushing crosses 100 mesh are weighed, are boiled by intense fire hereinafter
Fire boils 30min again, and filtered through gauze stays supernatant, filter residue that 200ml distilled water is added equally to handle 1 time again, residue is abandoned after filtering, merges 2
90 DEG C of water-baths of secondary filtrate progress are finally concentrated to 100ml (mass concentration is crude drug 1g/ml) and granatum extraction stoste are made；By institute
Granatum extraction stoste is diluted with water 5 times granatum leaching liquor is made.
The preparation of the compound seed soaking agent of 1 Trifolium repense of embodiment
Each ingredient (unit is weighed by following quality：g)：Gibberellin 1, sodium lignin sulfonate 1.4, vitamin K30.5；It will be upper
State each ingredient and be added in ethyl alcohol (content >=95%) and supply ethyl alcohol to 100g, stir to get.
The preparation of the compound seed soaking agent of 2 Trifolium repense of embodiment
Each ingredient (unit is weighed by following quality：g)：Gibberellin 0.5, sodium lignin sulfonate 1.2, vitamin K30.5；It will
Above-mentioned each ingredient, which is added in ethyl alcohol, supplies ethyl alcohol (content >=95%) to 100g, stir to get.
The preparation of the compound seed soaking agent of 3 Trifolium repense of embodiment
Each ingredient (unit is weighed by following quality：g)：Gibberellin 1.5, sodium lignin sulfonate 1.4, vitamin K30.6；It will
Above-mentioned each ingredient, which is added in ethyl alcohol, supplies ethyl alcohol (content >=95%) to 100g, stir to get.
The preparation of the compound seed soaking agent of 4 Trifolium repense of embodiment
Each ingredient (unit is weighed by following quality：g)：Gibberellin 0.8, sodium lignin sulfonate 1.4, vitamin K30.6；It will
Above-mentioned each ingredient, which is added in ethyl alcohol, supplies ethyl alcohol (content >=95%) to 100g, stir to get.
The preparation of the compound seed soaking agent of 5 Trifolium repense of embodiment
Each ingredient (unit is weighed by following quality：g)：Gibberellin 0.6, sodium lignin sulfonate 1.2, vitamin K30.5；It will
Above-mentioned each ingredient, which is added in ethyl alcohol, supplies ethyl alcohol (content >=95%) to 100g, stir to get.
The preparation of the compound seed soaking agent of 6 Trifolium repense of embodiment
The preparation of ingredient I：Each ingredient (unit is weighed by following quality：g)：Gibberellin 1, sodium lignin sulfonate 1.4, dimension life
Plain K30.5；Above-mentioned each ingredient is added in ethyl alcohol (content >=95%) and supplies ethyl alcohol to 100g, stir to get.
The preparation of ingredient II：Semen Cuscutae stoste prepared by Preparative Example 1 is diluted with water 3 times, obtains Semen Cuscutae extraction
Liquid, i.e. ingredient II.
By ingredient I and ingredient II according to 1:1000 mass ratio be uniformly mixed to get.
The preparation of the compound seed soaking agent of 7 Trifolium repense of embodiment
The preparation of ingredient I：Each ingredient (unit is weighed by following quality：g)：Gibberellin 0.8, sodium lignin sulfonate 1.2, dimension
Raw element K30.5；Above-mentioned each ingredient is added in ethyl alcohol and supplies ethyl alcohol (content >=95%) to 100g, is stirred, i.e.,
.
The preparation of ingredient II：Semen Cuscutae stoste prepared by Preparative Example 2 is diluted with water 4 times, obtains Semen Cuscutae extraction
Liquid, i.e. ingredient II.
By ingredient I and ingredient II according to 1:750 mass ratio be uniformly mixed to get.
The preparation of the compound seed soaking agent of 8 Trifolium repense of embodiment
The preparation of ingredient I：Each ingredient (unit is weighed by following quality：g)：Gibberellin 1, sodium lignin sulfonate 1.4, dimension life
Plain K30.6；Above-mentioned each ingredient is added in ethyl alcohol and supplies ethyl alcohol (content >=95%) to 100g, stir to get.
The preparation of ingredient II：Semen Cuscutae stoste prepared by Preparative Example 3 is diluted with water 4 times, obtains Semen Cuscutae extraction
Liquid, i.e. ingredient II.
By ingredient I and ingredient II according to 1:1250 mass ratio be uniformly mixed to get.
The screening test of the constituent of the compound seed soaking agent of 1 Trifolium repense of test example
(1) formula composition of compound seed soaking agent is respectively handled
According to primary screening test, find gibberellin, methyl α-naphthyl acetate, sodium lignin sulfonate, calcium lignosulfonate, vitamin C,
Vitamin K3Equal plant growth regulator and dodder extract have effects that hestening rooting germination；This experiment by gibberellin with
Methyl α-naphthyl acetate, sodium lignin sulfonate, calcium lignosulfonate, vitamin C, vitamin K3, the ingredients such as dodder extract are according to table 1
Mode carries out compatibility and obtains the Trifolium repense seed soaking agent of different disposal, and each processing is 1000 times of dilutions.
Table 1 respectively handles the formula composition and preparation and extension rate of compound seed soaking agent
| Processing | The preparation method of seed soaking agent | Dilution |
| 1 | Gibberellin 1g, sodium lignin sulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Semen Cuscutae stoste dilutes 3 times |
| 2 | Gibberellin 1g, calcium lignosulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Semen Cuscutae stoste dilutes 3 times |
| 3 | Gibberellin 1g, sodium lignin sulfonate 1.4g, vitamin C 0.5g, ethyl alcohol supply 100g | Semen Cuscutae stoste dilutes 3 times |
| 4 | Gibberellin 1g, sodium lignin sulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Cuscuta australis stoste dilutes 3 times |
| 5 | Gibberellin 1g, calcium lignosulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Cuscuta australis stoste dilutes 3 times |
| 6 | Gibberellin 1g, sodium lignin sulfonate 1.4g, vitamin C 0.5g, ethyl alcohol supply 100g | Cuscuta australis stoste dilutes 3 times |
| 7 | Gibberellin 1g, sodium lignin sulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Argy wormwood stoste dilutes 3 times |
| 8 | Gibberellin 1g, calcium lignosulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Argy wormwood stoste dilutes 3 times |
| 9 | Gibberellin 1g, sodium lignin sulfonate 1.4g, vitamin C 0.5g, ethyl alcohol supply 100g | Argy wormwood stoste dilutes 3 times |
| 10 | Gibberellin 1g, sodium lignin sulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Granatum stoste dilutes 3 times |
| 11 | Gibberellin 1g, calcium lignosulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Granatum stoste dilutes 3 times |
| 12 | Gibberellin 1g, sodium lignin sulfonate 1.4g, vitamin C 0.5g, ethyl alcohol supply 100g | Granatum stoste dilutes 3 times |
| 13 | Gibberellin 1g, sodium lignin sulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Tap water |
| 14 | Gibberellin 1g, calcium lignosulfonate 1.4g, Vitamin K3 0.5g, ethyl alcohol supply 100g | Tap water |
| 15 | Gibberellin 1g, sodium lignin sulfonate 1.4g, vitamin C 0.5g, ethyl alcohol supply 100g | Tap water |
| 16 | Blank (ethyl alcohol) | Tap water |
(2) it is tested on paper
The pretreatment of seed：It is the in the same size, full without incomplete Trifolium repense seed of Rui Wende to choose kind, is first used
0.1% disinfecting solution of potassium permanganate 10min, then with distilled water flushing 5-6 times, blotted with filter paper rear spare.By a diameter of 9cm
Culture dish cleaned 3 times with 75% alcohol, spread two layers of filter paper, be placed in 80 DEG C of baking oven baking 12h.Culture is added in distilled water 5ml
In ware, filter paper is made to reach saturation.Random that seed is taken uniformly to set bed, 100 seeds of each culture dish each handle 4 repetitions.It will
Culture dish is placed in illumination box, and condition of culture is：20 ± 1 DEG C of temperature, illumination 12h/d.
A same day start recording is set from seed, kind of skin 2mm is ejected as germination standard using plumule.Record germinative number daily,
The germinating energy of 7d statistics seeds is cultivated, 10d counts the germination percentage of seed.10d is sprouted, each processing takes 50 to sprout at random
The seed of hair, measures height of seedling and young root is long.
(3) Drought resistant experiment
Trifolium repense seed is selected to carry out Drought resistant experiment.Experiment handles (being shown in Table 1) for totally 16, and ethyl alcohol is blank control, each
Seed soaking agent carries out unified seed soaking (with being tested on paper).15% PEG-6000 solution 5ml is added in culture dish, is made
Filter paper solution reaches saturation.Random that seed is taken uniformly to set bed, 100 seeds of each culture dish each handle 4 repetitions.It will training
Foster ware is placed in illumination box, and condition of culture is：20 ± 1 DEG C of temperature, illumination 12h/d.10d is sprouted, each processing is random
The seed of 50 sproutings is taken, height of seedling is measured and young root is long.
(4) Salt tolerance
Trifolium repense seed is selected to carry out Salt tolerance.Experiment handles (being shown in Table 1) for totally 16, and ethyl alcohol is blank control, each
Seed soaking agent carries out unified seed soaking (with being tested on paper).0.2% NaCl solution 5ml is added in culture dish, filter is made
Paper solution reaches saturation.Random that seed is taken uniformly to set bed, 100 seeds of each culture dish each handle 4 repetitions.It will culture
Ware is placed in illumination box, and condition of culture is：20 ± 1 DEG C of temperature, illumination 12h/d.
A same day start recording is set from seed, kind of skin 2mm is ejected as germination standard using plumule.Record germinative number daily,
The germinating energy of 7d statistics seeds is cultivated, 10d counts the germination percentage of seed.10d is sprouted, each processing takes 50 to sprout at random
The seed of hair, measures height of seedling and young root is long.
Two, test result
Test result is shown in Table 2- tables 6 on paper, and resistance experiment (drought resisting, salt tolerant) the results are shown in Table 7- tables 12.
Test result shows gibberellin and sodium lignin sulfonate, vitamin K3With the leaching after dodder extract compatibility
Kind of agent (processing 1), compared to control (processing 16), Trifolium repense seedling germinating energy improve 61.24%, germination percentage improve 19.64%,
Drought resistance enhancing (root long increases by 76.32%), salt tolerance enhancing (root long increases by 55.12%).Gibberellin does not have the promotion of height of seedling
There are failure, sodium lignin sulfonate to be also not affected by influence to the promotion of root.According to test result as it can be seen that by gibberellin and lignin
Sodium sulfonate, vitamin K3Compound seed soaking agent collocation dodder extract after compatibility uses, and promotees to strengthen, increase promoting height of seedling, promoting root growth
Add the significant effect of salt tolerance and resistance etc. to be formed better than other compoundings and (handles 2-16, difference reaches extremely notable water
It is flat), this explanation, by gibberellin and sodium lignin sulfonate, vitamin K3It is compatible with extremely significant association with dodder extract
With synergy effect.
Test result on 2 paper of table
| Processing | Germinating energy (d) | Germination percentage (%) | Height of seedling (cm) | Young root grows (cm) |
| 1 | 69.22±2.68Aa | 97.93±2.63Aa | 3.02±0.1Aa | 2.76±0.08Aa |
| 2 | 53.05±0.93DEbcd | 88.4±3.25Bb | 2.52±0.11Bb | 2.15±0.04BCDbcd |
| 3 | 57.23±1.18BCDbc | 87.29±1.67Bbc | 2.47±0.05BCbc | 2.16±0.03BCDbcd |
| 4 | 56.94±0.43BCDbc | 82.75±2.29Bbc | 2.33±0.03Ccd | 2.18±0.05BCbc |
| 5 | 53.28±2.02DEbcd | 84.14±3.3Bbc | 2.3±0.04CDd | 2.14±0.06BCDEbcde |
| 6 | 56.08±1.53BCDEbc | 86.54±3.08Bbc | 2.39±0.07BCbcd | 2.19±0.08Bbc |
| 7 | 58.14±1.86BCb | 87.98±2.57Bbc | 2.41±0.02BCbcd | 2.21±0.08Bb |
| 8 | 56.6±1.33BCDbc | 85.98±1.09Bbc | 2.11±0.04Ee | 2.04±0.08BCDEcdef |
| 9 | 57.65±1.62BCDb | 86.97±2.15Bbc | 2.08±0.05Ee | 2.04±0.08BCDEcdef |
| 10 | 58.76±1.17Bb | 86.99±3.19Bbc | 2.31±0.06CDd | 2.1±0.05BCDEbcdef |
| 11 | 55.8±2.15BCDEbc | 83.72±1.6Bbc | 2.12±0.04Ee | 2.05±0.07BCDEcdef |
| 12 | 53.52±1.24DEcd | 84.76±2.64Bbc | 2.14±0.06DEe | 2.04±0.07BCDEcdef |
| 13 | 53.77±2.01CDEcd | 84.98±2.69Bbc | 2.04±0.06Ee | 1.98±0.06DEef |
| 14 | 51.85±0.82Ed | 81.52±2.37Bc | 2.01±0.03Ee | 1.99±0.06CDEdef |
| 15 | 53.54±1.55DEcd | 83.45±3.23Bbc | 2.03±0.04Ee | 1.98±0.07DEef |
| 16 | 42.93±1.4Fe | 82.69±2.29Bbc | 2.03±0.06Ee | 1.96±0.04Ef |
Germinating energy analysis of variance table is tested on 3 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 19.2168 | 3 | 6.4056 | 2.821 | 0.0495 |
| Between processing | 1393.4917 | 15 | 92.8994 | 40.912 | 0.0000 |
| Error | 102.1832 | 45 | 2.2707 | ||
| Total variation | 1514.8916 | 63 |
Germination percentage analysis of variance table is tested on 4 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 8.1403 | 3 | 2.7134 | 0.392 | 0.7592 |
| Between processing | 769.5561 | 15 | 51.3037 | 7.416 | 0.0000 |
| Error | 311.3095 | 45 | 6.918 | ||
| Total variation | 1089.0059 | 63 |
Height of seedling analysis of variance table is tested on 5 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0092 | 3 | 0.0031 | 0.836 | 0.4812 |
| Between processing | 4.1917 | 15 | 0.2794 | 76.244 | 0.0000 |
| Error | 0.1649 | 45 | 0.0037 | ||
| Total variation | 4.3658 | 63 |
The long analysis of variance table of young root is tested on 6 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0155 | 3 | 0.0052 | 1.244 | 0.305 |
| Between processing | 2.1415 | 15 | 0.1428 | 34.289 | 0.0000 |
| Error | 0.1874 | 45 | 0.0042 | ||
| Total variation | 2.3444 | 63 |
7 drought resisting of table, Salt tolerance result
| Processing | Drought resistant experiment height of seedling (cm) | Salt tolerance height of seedling (cm) |
| 1 | 2.49±0.09Aa | 2.62±0.08Aa |
| 2 | 2.07±0.04Bb | 2.1±0.05BCb |
| 3 | 2.02±0.07BCbc | 2.1±0.08Bb |
| 4 | 1.78±0.06DEd | 1.9±0.04Ed |
| 5 | 1.81±0.03Dd | 1.91±0.05DEd |
| 6 | 1.89±0.04CDcd | 1.95±0.06BCDEcd |
| 7 | 1.85±0.04CDd | 2.07±0.03BCDbc |
| 8 | 1.6±0.07Fe | 1.71±0.07Fe |
| 9 | 1.58±0.06Fe | 1.72±0.04Fe |
| 10 | 1.8±0.04Dd | 1.94±0.06CDEcd |
| 11 | 1.62±0.06EFe | 1.67±0.05Fef |
| 12 | 1.62±0.07EFe | 1.71±0.05Fe |
| 13 | 1.51±0.04Fe | 1.57±0.03Ff |
| 14 | 1.51±0.06Fe | 1.56±0.03Ff |
| 15 | 1.54±0.04Fe | 1.58±0.03Fef |
| 16 | 1.5±0.06Fe | 1.59±0.06Fef |
8 drought resisting of table, Salt tolerance result
| Processing | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) |
| 1 | 1.75±0.03Aa | 2.31±0.05Aa |
| 2 | 1.19±0.01BCbc | 1.71±0.04Bb |
| 3 | 1.19±0.03BCbc | 1.7±0.04BCb |
| 4 | 1.13±0.03CDcd | 1.68±0.04BCDEbcd |
| 5 | 1.19±0.03BCbc | 1.68±0.05BCDEbc |
| 6 | 1.21±0.04Bb | 1.69±0.03BCDb |
| 7 | 1.21±0.03Bb | 1.67±0.06BCDEFbcde |
| 8 | 1.05±0.01DEFef | 1.56±0.06CDEFGcdef |
| 9 | 1.06±0.02DEFdef | 1.59±0.02BCDEFGbcdef |
| 10 | 1.02±0.03EFef | 1.53±0.04FGf |
| 11 | 1.05±0.02DEFef | 1.55±0.07EFGef |
| 12 | 1.08±0.03DEde | 1.56±0.03DEFGdef |
| 13 | 1.01±0.03EFef | 1.49±0.05Gf |
| 14 | 1.01±0.03EFef | 1.51±0.03Gf |
| 15 | 0.99±0.02Ff | 1.5±0.04Gf |
| 16 | 1±0.04EFf | 1.49±0.06Gf |
9 Drought resistant experiment height of seedling analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0162 | 3 | 0.0054 | 1.769 | 0.1666 |
| Between processing | 4.1891 | 15 | 0.2793 | 91.707 | 0.0000 |
| Error | 0.137 | 45 | 0.003 | ||
| Total variation | 2.0573 | 63 |
10 Salt tolerance height of seedling analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0042 | 3 | 0.0014 | 0.486 | 0.6936 |
| Between processing | 4.7044 | 15 | 0.3136 | 108.573 | 0.0000 |
| Error | 0.13 | 45 | 0.0029 | ||
| Total variation | 4.8386 | 63 |
11 Drought resistant experiment root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0042 | 3 | 0.0014 | 1.994 | 0.1283 |
| Between processing | 2.0213 | 15 | 0.1348 | 191.106 | 0.0000 |
| Error | 0.0317 | 45 | 0.0007 | ||
| Total variation | 2.0573 | 63 |
12 Salt tolerance root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.002 | 3 | 0.0007 | 0.307 | 0.82 |
| Between processing | 2.3113 | 15 | 0.1541 | 69.92 | 0.0000 |
| Error | 0.0992 | 45 | 0.0022 | ||
| Total variation | 2.4125 | 63 |
The inspection test of the constituent of the compound seed soaking agent of 2 Trifolium repense of test example
One, test method
(1) formula composition of compound seed soaking agent is respectively handled
The constituent screening test of test example 1 the result shows that, sodium lignin sulfonate, vitamin K3It is extracted with Semen Cuscutae
It can promote the growth of young shoot after liquid compounding and there is no inhibiting effect to root, difference reaches extremely notable water compared with other processing
It is flat.It is extremely complex in view of the influence between plant growth regulator, it is understood that there may be a variety of influences such as synergy, antagonism, it is understood that there may be
Binary built is better than the case where ternary built.In addition, from the aspect of cost and preparation processing, reduce medicament kind also and have compared with
Apparent advantage is set so according to binary built and ternary built experiment is designed the case where test example 1 with reference to the dosage of table 13
Meter.
Table 13 respectively handles the formula composition and preparation and extension rate of compound seed soaking agent
(2) it is tested on paper
For specific method with test example 1, measurement index is root long.
(3) Drought resistant experiment
Specific method is the same as test example 1.
(4) Salt tolerance
Specific method is the same as test example 1.
Two, test result
Test result is shown in Table 14- tables 17.
According to test result as it can be seen that gibberellin, sodium lignin sulfonate, vitamin K3With the effect of dodder extract compounding
Other compound schemes (handling 2-7, difference reaches the pole level of signifiance) are significantly better than, are considered in conjunction with test example 1, it is red mould
Element, sodium lignin sulfonate and vitamin K3Compounding effect to be substantially better than other compound schemes, this shows the compound scheme
In three plant growth regulators and dodder extract between have apparent synergistic effect.
14 constituent inspection test result of table
| Processing | Root long (cm) is tested on paper | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) |
| 1 | 2.6±0.01Aa | 1.68±0Aa | 2.21±0.01Aa |
| 2 | 2.33±0.01Bb | 1.37±0.01Bb | 1.72±0.01Bb |
| 3 | 2.23±0.01Cc | 1.27±0.01Cc | 1.71±0Bb |
| 4 | 2.05±0.01Dd | 1.15±0.01Dd | 1.63±0.01Cc |
| 5 | 1.99±0.01Ff | 1.1±0Ee | 1.53±0.01Dd |
| 6 | 1.96±0Gg | 1.1±0Ee | 1.53±0Dd |
| 7 | 2.01±0.01Ee | 1.15±0.01Dd | 1.34±0.01Ff |
| 8 | 1.91±0.01Hh | 0.99±0.01Ff | 1.38±0.01Ee |
Root long analysis of variance table is tested on 15 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0002 | 3 | 0.0001 | 1.046 | 0.3929 |
| Between processing | 1.5598 | 7 | 0.2228 | 4303.007 | 0.0000 |
| Error | 0.0011 | 21 | 0.0001 | ||
| Total variation | 1.5611 | 31 |
16 Drought resistant experiment root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0001 | 3 | 0 | 1.296 | 0.3018 |
| Between processing | 1.3048 | 7 | 0.1864 | 11598.131 | 0.0000 |
| Error | 0.0003 | 21 | 0 | ||
| Total variation | 1.3052 | 31 |
17 Salt tolerance root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0001 | 3 | 0 | 1.15 | 0.3521 |
| Between processing | 2.0642 | 7 | 0.2949 | 9303.329 | 0.0000 |
| Error | 0.0007 | 21 | 0 | ||
| Total variation | 2.0649 | 31 |
The dosage screening test of the constituent of the compound seed soaking agent of 3 Trifolium repense of test example
One, test method
(1) the dosage design of the constituent of Trifolium repense seed soaking agent
According to the dosage 15 processing groups of design of table 18.
Table 18 respectively handles the preparation method of seed soaking agent
(2) it is tested on paper
For specific method with test example 1, measurement index is root long.
(3) Drought resistant experiment
Specific method is the same as test example 1.
(4) Salt tolerance
Specific method is the same as test example 1.
Two, test result
Test result is shown in Table 19- tables 22.
According to test result as it can be seen that the preferable compound scheme of effect is：Gibberellin 0.5%-1.5%+ sodium lignin sulfonates
1.2%-1.6%+ vitamin Ks30.4%-0.6% collocation dodder extract (Semen Cuscutae stoste dilutes 5 times), the compound scheme
It is better than other schemes outside the compound scheme range promoting root growth, resistance significant effect (difference reaches the level of signifiance)；
Wherein, " gibberellin 1%+ sodium lignin sulfonate 1.4%+ vitamin Ks3(Semen Cuscutae stoste is dilute for 0.5% collocation dodder extract
Release 3 times) " effect be best in compound scheme, be significantly better than in the compound scheme other formulas (difference reach extremely notable water
It is flat).
19 constituent dosage test result of table
| Processing | Root long (cm) is tested on paper | Drought resistant experiment root long (cm) | Salt tolerance root long (cm) |
| 1 | 2.73±0.05Aa | 1.85±0.07Aa | 2.29±0.07Aa |
| 2 | 2.43±0.06BCDbc | 1.56±0.04Bb | 2.01±0.08BCDbcd |
| 3 | 2.48±0.03Bb | 1.59±0.02Bb | 2.04±0.08BCbc |
| 4 | 2.36±0.06BCDEbcd | 1.5±0.06BCbcd | 2±0.06BCDbcde |
| 5 | 2.27±0.05CDEFcde | 1.44±0.05Ccd | 1.9±0.05BCDEFGcdef |
| 6 | 2.38±0.07BCDEbcd | 1.43±0.03CDcd | 1.9±0.05BCDEFcdef |
| 7 | 2.38±0.04BCDEbcd | 1.5±0.05BCbcd | 1.97±0.09BCDEbcde |
| 8 | 2.22±0.05EFde | 1.32±0.03DEef | 1.85±0.02DEFGefg |
| 9 | 2.16±0.06Fe | 1.28±0.05Ef | 1.81±0.04EFGfg |
| 10 | 2.27±0.06CDEFcde | 1.32±0.02DEef | 1.87±0.03CDEFGdefg |
| 11 | 2.12±0.05Fe | 1.22±0.01Ef | 1.73±0.03Gg |
| 12 | 2.14±0.09Fe | 1.25±0.03Ef | 1.78±0.06FGfg |
| 13 | 2.24±0.05DEFde | 1.42±0.03CDde | 1.88±0.05CDEFGdef |
| 14 | 2.16±0.09Fe | 1.25±0.04Ef | 1.77±0.06FGfg |
| 15 | 2.45±0.1BCb | 1.53±0.04BCbc | 2.07±0.05Bb |
Root long analysis of variance table is tested on 20 paper of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0072 | 3 | 0.0024 | 0.584 | 0.6286 |
| Between processing | 1.4966 | 14 | 0.1069 | 25.956 | 0.0000 |
| Error | 0.173 | 42 | 0.0041 |
| Total variation | 1.6768 | 59 |
21 Drought resistant experiment root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0051 | 3 | 0.0017 | 1.053 | 0.3794 |
| Between processing | 1.5711 | 14 | 0.1122 | 69.876 | 0.0000 |
| Error | 0.0675 | 42 | 0.0016 | ||
| Total variation | 1.6437 | 59 |
22 Salt tolerance root long analysis of variance table of table
| Source of variation | Quadratic sum | Degree of freedom | It is square | F values | The level of signifiance |
| Between district's groups | 0.0066 | 3 | 0.0022 | 0.65 | 0.5874 |
| Between processing | 1.1554 | 14 | 0.0825 | 24.218 | 0.0000 |
| Error | 0.1431 | 42 | 0.0034 | ||
| Total variation | 1.3052 | 59 |
Claims (14)
1. a kind of compound seed soaking agent of Trifolium repense, it is characterised in that：It is made of ingredient I and ingredient II；Wherein, the ingredient I is by red
Mycin, sodium lignin sulfonate, vitamin K3It is formed with ethyl alcohol；The ingredient II is dodder extract；According to mass ratio meter, at
Divide I:Ingredient II=1:500-1500；
By mass percentage, the dosage of each ingredient is in ingredient I：Gibberellin 0.5%-1.5%, sodium lignin sulfonate 1.2%-
1.6%, vitamin K30.4%-0.6%, surplus are ethyl alcohol；
The dodder extract is obtained after Semen Cuscutae stoste is diluted with water 1-5 times.
2. the compound seed soaking agent of Trifolium repense described in accordance with the claim 1, it is characterised in that：
Ingredient I:Ingredient II=1:750-1250；
The dosage of each ingredient is in ingredient I：Gibberellin 1%, sodium lignin sulfonate 1.4%, vitamin K30.5%；
The ethyl alcohol is the ethyl alcohol of content >=95%.
3. the compound seed soaking agent of Trifolium repense according to claim 2, it is characterised in that：Ingredient I:Ingredient II=1:1000.
4. the compound seed soaking agent of Trifolium repense described in accordance with the claim 1, it is characterised in that：The dodder extract is by Tu
The sub- stoste of silk obtains after being diluted with water 2-4 times.
5. the compound seed soaking agent of Trifolium repense according to claim 4, it is characterised in that：The dodder extract is by Tu
The sub- stoste of silk obtains after being diluted with water 3 times.
6. the compound seed soaking agent of Trifolium repense described in accordance with the claim 1, it is characterised in that：
The Semen Cuscutae stoste is prepared in accordance with the following methods：By Semen Cuscutae seed flooding to get Semen Cuscutae stoste.
7. the compound seed soaking agent of Trifolium repense according to claim 6, it is characterised in that：The Semen Cuscutae stoste is according to lower section
Method is prepared：
(1) it is decocted again after Semen Cuscutae mature seed being soaked in water；Decoction liquor is filtered, supernatant is taken, obtains filtrate 1；Filter residue is again
It is decocted with water, decoction liquor filtering takes supernatant, obtains filtrate 2；(2) filtrate 1 and filtrate 2 are merged, concentrate to get.
8. the compound seed soaking agent of Trifolium repense according to claim 7, it is characterised in that：In step (1), according to g：Ml is counted, Tu
The ratio of the sub- mature seed of silk and water is 0.5-2:1-8；In step (1), according to g：Ml is counted, and the ratio of filter residue and water is 0.5-2:
1-8；
It is concentrated again after carrying out 80-90 DEG C of water-bath after merging filtrate 1 and filtrate 2 in step (2).
9. the compound seed soaking agent of Trifolium repense according to claim 8, it is characterised in that：Semen Cuscutae mature seed in step (1)
Ratio with water is 1:2；The ratio of filter residue and water is 1 in step (1):2.
10. a kind of method preparing the compound seed soaking agent of Trifolium repense described in claim 1, which is characterized in that including：By ingredient I
With ingredient II be uniformly mixed to get.
11. the compound seed soaking agent of Trifolium repense described in claim 1-8 any one is shortening Trifolium repense growing-seedling period, is improving Trifolium repense
Emergence rate, promoting root growth promote the application in strong or raising Trifolium repense resistance aspect.
12. applying according to claim 11, which is characterized in that including：Trifolium repense seed is answered with the Trifolium repense
Seed soaking agent is closed to soak seed；Wherein, according to mass ratio meter, the ratio of Trifolium repense seed and the compound seed soaking agent of Trifolium repense is 1:3.
13. applying according to claim 12, it is characterised in that：The seed soaking is soaked seed at a temperature of 20 DEG C -30 DEG C
1-8 hours.
14. applying according to claim 13, it is characterised in that：The seed soaking is to soak seed 6 at a temperature of 20 DEG C -30 DEG C
Hour.
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| CN108323509A (en) * | 2018-01-22 | 2018-07-27 | 马鞍山市全润农业科技有限公司 | A kind of seed soaking agent and preparation method thereof for vegetable seeds |
| CN112567925A (en) * | 2020-12-11 | 2021-03-30 | 菏泽学院 | Method for improving salt stress resistance of lawn grass germination by gibberellin seed soaking |
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