CN105409749A - Water culturing method for rice root systems in seedling stage - Google Patents
Water culturing method for rice root systems in seedling stage Download PDFInfo
- Publication number
- CN105409749A CN105409749A CN201510908337.7A CN201510908337A CN105409749A CN 105409749 A CN105409749 A CN 105409749A CN 201510908337 A CN201510908337 A CN 201510908337A CN 105409749 A CN105409749 A CN 105409749A
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- Prior art keywords
- seed
- culture dish
- toilet paper
- water
- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention discloses a water culturing method for rice root systems in a seedling stage. According to the method, toilet paper is used as a support medium, growth of rice in soil is imitated, thus good rice root systems for root system study can be obtained by the method. By the method, disadvantages of a supporting medium for culturing root systems in the traditional water culturing method are overcome; the method is simple and speedy, has incomparable advantages on the study of the rice root systems in the seedling stage, and has important practical significance on the study of the rice root systems.
Description
Technical field
The present invention relates to a kind of rice seedling root system ciltivating process.
Background technology
Root system of plant comparatively acrial part is compared, and structure is relatively simple, but it has very important meaning to plant, is providing support effect of plant growth, needed for the confession plant forms such as the moisture of simultaneously stability underground, mineral salt and organic matter build up.But root system of plant research is at present less, traces it to its cause, be because the Measures compare of root system research falls behind.Current root system research has the methods such as husky training, water planting.Root is long, the quantity of root and distribution, root/shoot ratio, root system development etc. to require the index obtained mostly to be to the research of root system.When studying these indexs, Sha Pei etc. have the cultural method of medium, and often root system is very difficult to obtain, and the index recorded is difficult to accurately.And the advantage that water culture has other method incomparable on root system is cultivated, but also there is complex operation etc., various shortcoming due to water culture, be difficult to the simple and quick required root system of acquisition experiment.Cultivate the shortcoming of root system based on water culture, the present invention improves, and makes the cultivation of its applicable rice seedling root system.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of rice seedling root system ciltivating process.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is, a kind of rice seedling root system ciltivating process, comprises the following steps:
(1) select full seed, healthy seed, uses 10% hypochlorite disinfectant, and clear water rinses 4 ~ 5 times, after 33 DEG C of vernalization show money or valuables one carries unintentionally, is seeded in culture dish; Spread at least five layers of circular toilet paper identical with culture dish diameter bottom culture dish, add water to all toilet papers completely moistening, seed is placed on toilet paper uniformly;
(2) after planting at the toilet paper of seed upper cover and bottom same diameter, object is moisturizing and imitate the environment that seed grows in soil in the process of seed growth;
(3) after planting seed, little watering can is used to add water above culture dish, to water did not have seed;
(4) be placed in illumination box by culture dish and cultivate, the intensity of illumination is at 80 ~ 120 μm of ol/m
2between s, temperature 25 DEG C, humidity 70% ~ 80%, every day irradiation 16h; Use pH value be 6.5 Kimura's nutrient solution cultivate, every 3d changes a culture fluid;
(5) water planting basis of time experiment purpose determines, when getting root, mentions paddy rice stem, puts into clear water and rinse, can obtain complete rice root.
As preferably, in step (1), at diameter 20cm, the circular toilet paper that bottom the culture dish of high 5cm, paving 10 ~ 15 layers is identical with culture dish diameter, add water to all toilet papers completely moistening, be placed in uniformly on toilet paper by seed, 20 seeds sowed by each culture dish.
As preferably, in step (2), after planting at the toilet paper 1 ~ 2 layer of seed upper cover and bottom same diameter.
The invention has the beneficial effects as follows:
1, utilize toilet paper as medium, can provide support for paddy growth, ensure that Rooted Cuttings normal upright grows.
2, employing all spreads toilet paper up and down, has imitated the state that paddy rice grows in soil, has served the effect of moisturizing simultaneously.
3, toilet paper easily absorbs water and frangible feature after meeting water, can provide good environment, ensure that root system normal growth for root growth and lower bundle, and the root table face not easily lignification of simultaneously cultivating like this, is conducive to root system research.
4, complete root system can be obtained by simple clear water flushing, for the accuracy of rice root research provides guarantee.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is three kinds of ciltivating process comparative results of rice seedling root system ciltivating process embodiment of the present invention.
Embodiment
In an embodiment, ciltivating process of the present invention (i.e. toilet paper method) and other ciltivating process of two kinds (i.e. vermiculite method and blotting paper method) are compared, result is as follows:
1, the difference of three kinds of ciltivating process: toilet paper method lays toilet paper in culture dish, and vermiculite method and blotting paper rule lay vermiculite and blotting paper respectively in culture dish.
2, after material water planting of participating in the experiment proceeded to for 3 leaf 1 heart stages, vermiculite method and blotting paper method and toilet paper ciltivating process of the present invention are compared, ciltivating process of the present invention, can obtain good root system.
As shown in Figure 1, toilet paper ciltivating process of the present invention toilet paper method in white root, the longest root length, root quantity etc. all has good performance, and all exceedes vermiculite method and blotting paper method.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.
Claims (3)
1. a rice seedling root system ciltivating process, comprises the following steps:
(1) select full seed, healthy seed, uses 10% hypochlorite disinfectant, and clear water rinses 4 ~ 5 times, after 33 DEG C of vernalization show money or valuables one carries unintentionally, is seeded in culture dish; Spread at least five layers of circular toilet paper identical with culture dish diameter bottom culture dish, add water to all toilet papers completely moistening, seed is placed on toilet paper uniformly;
(2) after planting at the toilet paper of seed upper cover and bottom same diameter, object is moisturizing and imitate the environment that seed grows in soil in the process of seed growth;
(3) after planting seed, little watering can is used to add water above culture dish, to water did not have seed;
(4) be placed in illumination box by culture dish and cultivate, the intensity of illumination is at 80 ~ 120 μm of ol/m
2between s, temperature 25 DEG C, humidity 70% ~ 80%, every day irradiation 16h; Use pH value be 6.5 Kimura's nutrient solution cultivate, every 3d changes a culture fluid;
(5) water planting basis of time experiment purpose determines, when getting root, mentions paddy rice stem, puts into clear water and rinse, can obtain complete rice root.
2. rice seedling root system ciltivating process according to claim 1, it is characterized in that: in step (1), at diameter 20cm, the circular toilet paper that bottom the culture dish of high 5cm, paving 10 ~ 15 layers is identical with culture dish diameter, add water to all toilet papers completely moistening, be placed in uniformly on toilet paper by seed, 20 seeds sowed by each culture dish.
3. rice seedling root system ciltivating process according to claim 1, is characterized in that: in step (2), after planting at the toilet paper 1 ~ 2 layer of seed upper cover and bottom same diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510908337.7A CN105409749A (en) | 2015-12-10 | 2015-12-10 | Water culturing method for rice root systems in seedling stage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510908337.7A CN105409749A (en) | 2015-12-10 | 2015-12-10 | Water culturing method for rice root systems in seedling stage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105409749A true CN105409749A (en) | 2016-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510908337.7A Pending CN105409749A (en) | 2015-12-10 | 2015-12-10 | Water culturing method for rice root systems in seedling stage |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718777A (en) * | 2016-11-15 | 2017-05-31 | 江西省红壤研究所 | A kind of rice at whole growth periods hydroponics System and method for |
| CN108782202A (en) * | 2018-06-22 | 2018-11-13 | 湖南农业大学 | A method of adjusting rice leaf Determination of Chlorophyll content |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511365A (en) * | 2011-12-15 | 2012-06-27 | 上海市农业生物基因中心 | Method for measuring configuration and morphology of rice seedling root |
| CN102860199A (en) * | 2012-09-03 | 2013-01-09 | 安徽省农业科学院水稻研究所 | Rapid screening method for drought-enduring variety of rice at seedling stage |
| CN102907306A (en) * | 2012-11-20 | 2013-02-06 | 天津农学院 | Method for producing trigonella foenum sprouting vegetable |
-
2015
- 2015-12-10 CN CN201510908337.7A patent/CN105409749A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511365A (en) * | 2011-12-15 | 2012-06-27 | 上海市农业生物基因中心 | Method for measuring configuration and morphology of rice seedling root |
| CN102860199A (en) * | 2012-09-03 | 2013-01-09 | 安徽省农业科学院水稻研究所 | Rapid screening method for drought-enduring variety of rice at seedling stage |
| CN102907306A (en) * | 2012-11-20 | 2013-02-06 | 天津农学院 | Method for producing trigonella foenum sprouting vegetable |
Non-Patent Citations (6)
| Title |
|---|
| 庞莉萍等: "《丰乐辣椒新品种栽培技术》", 31 May 2001 * |
| 廖建良等: "CCFL 补光对四季豆栽培影响研究", 《惠州学院学报（自然科学版）》 * |
| 张显波: "几种处理方法对火麻种子萌芽率的影响", 《种业导刊》 * |
| 李志宇: "干旱胁迫对不同抗旱性陆稻幼苗根系活力的影响", 《中国农业信息》 * |
| 陈勇等: "萝卜芽苗菜水培比较试验", 《上海蔬菜》 * |
| 马廷臣等: "PEG-6000模拟干旱对水稻苗期根系形态和部分生理指标影响的研究", 《中国农学通报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718777A (en) * | 2016-11-15 | 2017-05-31 | 江西省红壤研究所 | A kind of rice at whole growth periods hydroponics System and method for |
| CN106718777B (en) * | 2016-11-15 | 2020-06-05 | 江西省红壤研究所 | Soilless planting system and method for rice in whole growth period |
| CN108782202A (en) * | 2018-06-22 | 2018-11-13 | 湖南农业大学 | A method of adjusting rice leaf Determination of Chlorophyll content |
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Application publication date: 20160323 |