CN105961175A - Arabidopsis sterile water culture device and method - Google Patents
Arabidopsis sterile water culture device and method Download PDFInfo
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- CN105961175A CN105961175A CN201610516251.4A CN201610516251A CN105961175A CN 105961175 A CN105961175 A CN 105961175A CN 201610516251 A CN201610516251 A CN 201610516251A CN 105961175 A CN105961175 A CN 105961175A
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- arabidopsis
- hydroponic device
- aseptic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses an arabidopsis sterile water culture device and method, and belongs to the technical field of plant culture. The device comprises a culture box and a box cover. The culture box is transparent and contains a nutrient solution. A groove is formed in the inner wall of the culture box. An iron gauze is arranged on the groove and supported by the groove. A set of gauze holes are evenly formed in the iron gauze. The hole diameter of the gauze holes is smaller than that of arabidopsis seeds. The arabidopsis seeds are fixed to the gauze holes. The device is simple in structure, convenient to operate, low in cost, low in material consumption and high in using flexibility; a water culture system with the nutrient solution and sterile equipment combined is adopted for the water culture device to be used for culturing arabidopsis seeds, the arabidopsis seeds are fixed to the iron gauze and then make sufficient contact with the nutrient solution, the advantages of water culture planting are reserved, the defect that experiment equipment is tedious and prone to contamination is overcome, sterile growth environment of arabidopsis can be ensured, and fungus and algal pollution is greatly reduced.
Description
Technical field
The invention belongs to field of plant growing technology, a kind of aseptic hydroponic device of arabidopsis being applicable to direct sowing method and aseptic culture and ciltivating process thereof.
Background technology
Arabidopsis has easily cultivation, and inexpensive, the advantages such as the breeding cycle is short, frequently as model organism important in hereditism and molecular biology research.Owing to arabidopsis gene group minimum, high homogenous, and genome sequencing in known plants have worked, in plant gene function, regulatory mechanism and environmental toxicology research, play irreplaceable effect.Along with the development of omics technology, Plant Physiology research has been enter into big data age, and this seedling that rapid, high volume of arabidopsis is cultivated high-quality is had higher requirement.
Arabidopsis seed is little, and Seedling is weak, and growing environment is had strict demand, has higher requirement to training method.The training method of Arabidopsis thaliana Seedlings mainly has water culture, earth culture method (Nutrition Soil or medium mixed culture) and tissue culture method at present.Earth culture and group training method plant are more thin and weak, and plant bolting is unstable, lodging phenomenon easily occur, and can not be strict the nutritional labeling controlling culture medium, be unfavorable for observation and the research of root system of the whole growth cycle of plant, cause many obstructions to research work.
Water culture is the most simple to operate, can accurate manual control nutrition condition of culture, fast culture goes out neat seedling, and each organ of plant easily obtains.Therefore, water culture is that plant nutrient coerces the important means studied with plant metabolism etc..
The ciltivating process of arabidopsis goes back some deficiency on motility and condition control at present, and equipment is more complicated, and necessary for growth is transplanted, thus causes certain physical damnification.It addition, traditional live water culture seed is difficult to fixing and experimental provision open design is vulnerable to many pollutions such as algae, fungus.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to provide a kind of aseptic hydroponic device of arabidopsis and ciltivating process thereof.It uses the hydroponic system that nutritional solution combines with sterile equipment, the advantage not only remaining water planting plantation, and overcomes the defect of the loaded down with trivial details easy microbiological contamination of experimental facilities.
The described aseptic hydroponic device of a kind of arabidopsis, including the cultivation box being equipped with and lid, cultivation box is transparent cartridge, cultivate box built with nutritional solution, it is characterized in that cultivating box inwall is provided with groove, wire gauze is arranged on groove and is supported by groove, and wire gauze is evenly arranged with a networking hole, mesh aperture is less than arabidopsis seed diameter, and arabidopsis seed is fixed on mesh.
The described aseptic hydroponic device of a kind of arabidopsis, it is characterised in that lid is provided with 0.45 μm microporous filter membrane, in the entrance hydroponic device by microporous filter membrane isolation microbes in air.
The described aseptic hydroponic device of a kind of arabidopsis, it is characterised in that described 0.45 μm microporous filter membrane area and lid area ratio are 1:4-6.
The described aseptic hydroponic device of a kind of arabidopsis, it is characterized in that described cultivation box is variable-diameter structure, being connected and composed by upper cartridge body and lower box body, upper cartridge body and lower box body are post cone table structure, and groove is arranged on the junction of the small-bore one end of upper cartridge body and lower box body heavy caliber one end.
The described aseptic hydroponic device of a kind of arabidopsis, it is characterised in that described lid area is area matched with upper cartridge body heavy caliber one end, lid height is less than or equal to the height to groove suitable for reading of upper cartridge body.
The arabidopsis sterilized water culture method of the aseptic hydroponic device of described arabidopsis, it is characterised in that comprise the following steps:
1) assembling hydroponic device, pour the culture medium of sterilizing in lower box body into, interpolation to liquid level is fully contacted with wire gauze, in order to arabidopsis after planting can contact with nutritional solution at once, it is thus achieved that the nutrition required for growth；
2) drawing the arabidopsis seed after sterilizing with liquid-transfering gun, plantation point as required, in the mesh of wire gauze, keeps seed to be fully contacted with nutritional solution, fixing lid；Subsequently hydroponic device is put into temperature and is 24-30 DEG C, relative humidity be the dark culturing case one day of 60-80% after it germinates, take temperature and be 22-25 DEG C, cultivate between the cultivation of relative humidity 50-80%, until arabidopsis grows into required size, for experiment.
Described arabidopsis sterilized water culture method, it is characterized in that described culture medium is containing 10mL a great number of elements A1 mother solution in every liter of culture fluid, 10mL a great number of elements A2 mother solution, 0.1mL trace element B1 mother solution, 0.1mL trace element B2 mother solution and iron salt 5mL mother solution, surplus is deionized water, and its pH value is 5.60.
Described arabidopsis sterilized water culture method, it is characterised in that described a great number of elements A1 mother solution contains KNO3 60.66 g/L, (NH4)H2PO4
1.1503 g/L, MgSO4·7H2O 24.647 g/L；Described a great number of elements A2 mother solution contains Ca (NO3)2·4 H2O 94.46 g/L；Described trace element B1 mother solution contains KCl 18.6375 g/L；Described trace element B2 mother solution contains H3BO3 1.9323
G/L, MnSO4·H2O 0.4226 g/L, ZnSO4•7 H2O 0.7189
G/L, CuSO4•5 H2O 0.3121
G/L, H2MoO4 0.0405
G/L, NiSO4•6 H2O 0.0657
g/L；Described mother liquid of iron salt contains EDTA 2Na 2 H2O 3.7224
G/L, FeCl3·6 H2O 2.703 g/L。
Described arabidopsis sterilized water culture method, it is characterised in that arabidopsis seed to be sowed after 3 days at 4-6 DEG C in first vernalization again.
Described arabidopsis sterilized water culture method, it is characterised in that the periodicity of illumination between described cultivation is that 16-18h illumination/6-8h is dark, its intensity of illumination 3000-5000lux.
By using above-mentioned technology, compared with prior art, the invention have the advantages that and effect:
1) hydroponic device of the present invention uses the wire gauze with many mesh to be used for fixing arabidopsis seed, its cultivation is the most efficient, different densities plantation can be met, obtain the Arabidopsis thaliana Seedlings that a large amount of growing way is consistent at short notice, it is provided without the fixing material of the auxiliary such as hollow pipe or sponge, its simple in construction, easy to operate, low cost, consumptive material is few, use motility is high, it is by fixing arabidopsis seed with wire gauze, fully contact with nutritional solution, and the repeatable utilization of wire gauze, high temperature resistant can sterilizing, quality is hard to be unlikely to deform, Arabidopsis thaliana Seedlings can be met cultivate, it is suitable for the plantation of large-scale water planting, meet especially and scientific research requiring, obtaining aseptic Arabidopsis thaliana Seedlings cultivates, it is equally applicable to the plant that other seeds are tiny；
2) present invention is by using this hydroponic device to use the hydroponic system that nutritional solution combines with sterile equipment, for cultivating arabidopsis seed, its arabidopsis seed is fixed on wire gauze, directly it is fully contacted with nutritional solution again, the advantage not only remaining water planting plantation, and overcome the defect of the loaded down with trivial details easy microbiological contamination of experimental facilities, it is ensured that and the sterile growth environment of arabidopsis, greatly reduce the pollution of mushroom and algae；
3) present invention defines 0.45 μm microporous filter membrane is set on lid, and to further define 0.45 μm microporous filter membrane area with lid area ratio be 1:4-6, effectively completely cut off the entrance of microbes in air.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the cultivation box structural representation of the present invention；
Fig. 3 is the wire gauze structural representation of the present invention.
In figure: 1-cultivates box, 101-groove, 102-upper cartridge body, 103-lower box body, 2-wire gauze, 201-mesh, 3-lid.
Detailed description of the invention
Below in conjunction with Figure of description, the invention will be further described, but protection scope of the present invention is not limited to that.
As Figure 1-3, the aseptic hydroponic device of a kind of arabidopsis of the present invention, use for cultivating Arabidopsis thaliana Seedlings, it includes cultivation box 1 and the lid 3 being equipped with, and cultivating box 1 is transparent cartridge, and this cultivation box 1 is variable-diameter structure, connected and composed by the upper cartridge body 102 and lower box body 103 being post cone table structure, wherein the small-bore one end of upper cartridge body 102 is connected with lower box body 103 heavy caliber one end, and groove 101 is arranged on junction, and lower box body 103 is used for containing nutritional solution；Lid 3 area is area matched with upper cartridge body 102 heavy caliber one end, lid 3 height is less than or equal to cultivate the height to groove 101 suitable for reading of the upper cartridge body 102 of box 1 to ensure to seal, wire gauze 2 is arranged on groove 101 and is supported by groove 101, a networking hole 201 it is evenly arranged with on wire gauze 2, mesh 201 aperture is less than or equal to arabidopsis seed diameter, arabidopsis seed is fixed on mesh 201, mesh 201 without all of, can according to experiment the live density of Demand Design.
Having through hole on the lid 3 of the present invention, through hole is provided with 0.45 μm microporous filter membrane, and in the entrance hydroponic device by microporous filter membrane isolation microbes in air, described 0.45 μm microporous filter membrane area and lid 3 area ratio are 1:4-6, preferably 1:5.
The present invention passes through the wire gauze 2 supporter as arabidopsis seed of recoverable, cost and consumptive material are saved, and can flexible operating, utilize aseptic lid 3, can the substantial amounts of aseptic Arabidopsis thaliana Seedlings of fast culture, the shortcoming solving the interference of the easy algal suspension of existing hydroponic device and microorganism, and cultivation box 1 is made of clear material, the convenient change observing plant root, its simple in construction, motility is high, easy to operate, low cost consumptive material is few, quick and substantial amounts of can provide aseptic vegetable material for scientific research.
Embodiment 1
As it can be seen, a kind of arabidopsis ciltivating process of the aseptic hydroponic device of arabidopsis limited based on the present invention of the present invention, specifically include following steps:
1) assembling hydroponic device, and add the culture medium that sterilizing is good in lower box body 103, culture medium liquid level is fully contacted with wire gauze 2；
2) arabidopsis seed, first vernalization 3 days under 4-6 DEG C (with 4 DEG C in embodiment), (arabidopsis seed is initially charged in a small amount of culture medium to draw arabidopsis seed with 10mL liquid-transfering gun, it is easy to it be drawn), program request is in the mesh 201 of wire gauze 2, it is made to be fully contacted with the nutritional solution in lower box body in step 1) 103, it is 24-30 DEG C (with 25 DEG C in embodiment) that hydroponic device is put into temperature subsequently, relative humidity be the dark culturing case one day of 60-80% after it germinates, then this hydroponic device is put into and cultivates between cultivation；Condition of culture between described cultivation is: periodicity of illumination 16-18h illumination/6-8h is dark, temperature is about 22-25 DEG C, and (the periodicity of illumination 16h illumination in embodiment/8h is dark, temperature is about 22-25, relative humidity is 50-80%), by using this experimental provision to cultivate arabidopsis, its germination percentage can reach more than 90%, and planting percent more than 80%, within pollution rate can be controlled in 5%.
Above-described embodiment is the embodiment that the present invention is optimal; but embodiments of the present invention are also not restricted to the described embodiments; the change made under other any spirit and principle without departing from invention, modify, substitute, combine, simplify; all should be the substitute mode of equivalence, be included in protection scope of the present invention.
Claims (10)
1. the aseptic hydroponic device of arabidopsis, including the cultivation box (1) being equipped with and lid (3), cultivating box (1) is transparent cartridge, cultivate box (1) built with nutritional solution, it is characterized in that cultivating box (1) inwall is provided with groove (101), wire gauze (2) is arranged on groove (101) and above and is supported by groove (101), a networking hole (201) it is evenly arranged with on wire gauze (2), mesh (201) aperture is less than arabidopsis seed diameter, and arabidopsis seed is fixed on mesh (201).
A kind of aseptic hydroponic device of arabidopsis the most according to claim 1, it is characterised in that lid (3) is provided with 0.45 μm microporous filter membrane, in the entrance hydroponic device by microporous filter membrane isolation microbes in air.
A kind of aseptic hydroponic device of arabidopsis the most according to claim 2, it is characterised in that described 0.45 μm microporous filter membrane area and lid (3) area ratio are 1:4-6.
A kind of aseptic hydroponic device of arabidopsis the most according to claim 1, it is characterized in that described cultivation box (1) is variable-diameter structure, connected and composed by upper cartridge body (102) and lower box body (103), upper cartridge body (102) and lower box body (103) are post cone table structure, and groove (101) is arranged on the junction of upper cartridge body (102) small-bore one end and lower box body (103) heavy caliber one end.
A kind of aseptic hydroponic device of arabidopsis the most according to claim 4, it is characterized in that the area matched of described lid (3) area and upper cartridge body (102) heavy caliber one end, lid (3) height is less than or equal to the height to groove (101) suitable for reading of upper cartridge body (102).
6. an arabidopsis sterilized water culture method based on the aseptic hydroponic device of the arabidopsis described in claim 1, it is characterised in that comprise the following steps:
1) assembling hydroponic device, pour the culture medium of sterilizing in lower box body (103) into, interpolation to liquid level is fully contacted with wire gauze (2), in order to arabidopsis after planting can contact with nutritional solution at once, it is thus achieved that the nutrition required for growth；
2) drawing the arabidopsis seed after sterilizing with liquid-transfering gun, plantation point as required, in the mesh (201) of wire gauze (2), keeps seed to be fully contacted with nutritional solution, fixing lid (3)；Subsequently hydroponic device is put into temperature and is 24-30 DEG C, relative humidity be the dark culturing case one day of 60-80% after it germinates, take temperature and be 22-25 DEG C, cultivate between the cultivation of relative humidity 50-80%, until arabidopsis grows into required size, for experiment.
Arabidopsis sterilized water culture method the most according to claim 6, it is characterized in that described culture medium is containing 10mL a great number of elements A1 mother solution in every liter of culture fluid, 10mL a great number of elements A2 mother solution, 0.1mL trace element B1 mother solution, 0.1mL trace element B2 mother solution and iron salt 5mL mother solution, surplus is deionized water, and its pH value is 5.60.
Arabidopsis sterilized water culture method the most according to claim 7, it is characterised in that described a great number of elements A1 mother solution contains KNO3 60.66 g/L, (NH4)H2PO4
1.1503 g/L, MgSO4·7H2O 24.647 g/L；Described a great number of elements A2 mother solution contains Ca (NO3)2·4 H2O 94.46 g/L；Described trace element B1 mother solution contains KCl 18.6375 g/L；Described trace element B2 mother solution contains H3BO3 1.9323
G/L, MnSO4·H2O 0.4226 g/L, ZnSO4•7 H2O 0.7189
G/L, CuSO4•5 H2O 0.3121
G/L, H2MoO4 0.0405
G/L, NiSO4•6 H2O 0.0657
g/L；Described mother liquid of iron salt contains EDTA 2Na 2 H2O 3.7224
G/L, FeCl3·6 H2O 2.703 g/L。
Arabidopsis sterilized water culture method the most according to claim 6, it is characterised in that arabidopsis seed to be sowed after 3 days at 4-6 DEG C in first vernalization again.
Arabidopsis sterilized water culture method the most according to claim 6, it is characterised in that the periodicity of illumination between described cultivation is that 16-18h illumination/6-8h is dark, its intensity of illumination 3000-5000lux.
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| CN201610516251.4A CN105961175A (en) | 2016-07-04 | 2016-07-04 | Arabidopsis sterile water culture device and method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106973772A (en) * | 2017-05-11 | 2017-07-25 | 武汉大学 | A kind of transgenic arabidopsis hydroponic device and its ciltivating process that can be recycled |
| CN107360958A (en) * | 2017-09-05 | 2017-11-21 | 中国农业科学院麻类研究所 | A kind of method of hemp fast-propagation |
| CN107637491A (en) * | 2017-09-22 | 2018-01-30 | 浙江省林业科学研究院 | Suitable for physiology and the arabidopsis cultural method and culture apparatus special of genetic research |
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