CN105409703A - Method for enhancing cold resistance of Kandelia obovata - Google Patents
Method for enhancing cold resistance of Kandelia obovata Download PDFInfo
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Abstract
The invention discloses a method for enhancing the cold resistance of Kandelia obovata. According to the method, an artificial accurate phased cooling method is used for enhancing the cold resistance of the Kandelia obovata, so that the survival rate of the Kandelia obovata in a low-temperature environment can be increased, and a transplanting process of the Kandelia obovata to the high latitude is promoted. The core concept of the technical scheme is as follows: the environment temperature is reduced continuously in a seedling culture process while the seedling culture effect of the Kandelia obovata is guaranteed, the adaptability of the Kandelia obovata to the low temperature is improved gradually, and the cold resistance of Kandelia obovata is improved. According to the method, Kandelia obovata seedlings are promoted to perform vigorous photosynthesis by means of a high temperature close to the outside before the environment temperature is adjusted to be low, and nutrients, namely, osmotic adjustment substances including sugar and amino acid facilitating relief of low-temperature stress are generated; then through phased gradient cooling, osmotic substance accumulation can be promoted, the ratio of bound water to free water is increased, the content of unsaturated fatty acid in membrane lipid is increased, phase change of a biofilm is prevented effectively, and the cold resistance of the Kandelia obovata is enhanced.
Description
The application is to Chinese invention number of patent application: 201410505663.9, the applying date: on September 27th, 2014, title: the divisional application improving the cold resistant method of autumn eggplant.
Technical field
The present invention relates to a kind of method strengthening autumn eggplant cold resistance, is exactly utilize the mode of control temperature to strengthen the method for autumn eggplant tolerance to cold.
Background technology
Mangrove forest is the special wetlands ecosystems being in the torrid zone, land, subtropics and ocean intermediate zone, is the ecologically criticalarea of littoral zone, has biometric safeguard diversity, opposing tsunami and the important ecological functions such as typhoon, region climate comfort.Under Global climate change condition, frequency and the ever-increasing extreme low temperature weather of intensity cause mangrove ecosystem mechanism to be degenerated, and cause that mangrove forest is withered and yellow, fallen leaves, even dead, exacerbate the atrophy of Mangrove Wetlands.Therefore, the research of strengthening improving mangrove forest tolerance to cold is badly in need of, to promote China's southeastern coast shelterbelt construction and littoral zone ecological system degradation.
In recent years, due to the impact that mankind's activity and climatic variation bring, China's mangrove forest area is still in continuous minimizing.Compared with the 1950's, China's mangrove forest area loses 73%.Along with global warming is more and more obvious, mangrove forest distribution also creates change, and artificial graft woods is to more high latitude expansion, and this occurs mangrove forest by making the original area that cannot grow mangrove forest because of temperature limiting.Therefore, under the background of global warming, expand mangrove forest and also will become one of increase China mangrove forest area trend to the plantation of more high latitude.
Autumn eggplant (Kandeliaobovata) is China's not only common but also the widest mangrove that distributes, and its artificial planting grows into forest the most northern to island, Yueqing bay Zhejiang Province west gate, has extremely strong adaptive capacity, has become one of Major Tree Species Planted of China's southeastern coast.But the extreme low temperature that Wintering Period occurs still affects introduces a fine variety surviving and growing of autumn eggplant, the Mangrove seedling major part that cold damage as occurred at the beginning of 2011 causes Wenzhou to transplant for 2008 is later dead, becomes China's Kandelia candelforest to more high latitude manually one of main limiting factor of introducing a fine variety success.Therefore, how to strengthen autumn eggplant tolerance to cold, the survival ability of maintenance cryogenic conditions lower autumn eggplant just becomes the bottleneck problem to more high latitude artificial afforestration.
Summary of the invention
Technical problem to be solved by this invention is: utilize the method for artificially accurately interim cooling to strengthen the tolerance to cold of autumn eggplant, improve autumn eggplant survival rate at low ambient temperatures, thus promotion autumn eggplant is to the transplanting process of high latitude with this.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
(a), on the beach of river mouth or littoral zone, take ooze, removing ooze in rubble and root system of plant after, after natural air drying is broken into pieces, be loaded on be convenient to carrying and in the watertight cultivating container in bottom, getting harmless ripe autumn eggplant plumular axis inserts in cultivating container, pouring NaCl concentration is the nutrient solution of 10 ‰, keeps inner bucket water layer 1-2cm;
(b), the cultivating container being inserted with autumn eggplant plumular axis is put into climatic cabinate, adjustment climatic cabinate parameter be: temperature 13 ~ 15 DEG C of (daytime)/9 ~ 10 DEG C (night), relative moisture control to be more than or equal to 600 μm of olm in 50%-70%, intensity of illumination
-2s
-1, every day, daylight was according to 11 ~ 13h, cultivated 2 ~ 3 days,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), light application time, intensity and relative moisture are all constant;
(c), when autumn eggplant plumular axis grow two leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), cultivate 2 ~ 3 days, illumination and relative moisture are all constant,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), and illumination and relative moisture are still all constant;
(d), when autumn eggplant plumular axis grow to four leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture are all constant, cultivate 2 ~ 3 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), and illumination and relative moisture are all constant, cultivates 3 ~ 4 days,
Continue adjustment climatic cabinate parameter be: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), illumination and relative moisture constant;
(e), when autumn eggplant plumular axis grow to six leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 5 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 5 days,
Continue adjustment climatic cabinate parameter be: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), illumination and relative moisture constant;
(f), when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 6 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 6 days,
Continue adjustment climatic cabinate parameter be: temperature 9 ~ 11 DEG C of (daytime)/5 ~ 6 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 ~ 6 days,
Wherein the management process of (b), (c), (d), (e), (f) each step respectively: supplement not containing the nutrient solution of NaCl in time, keep water layer 1-2cm, period also ensures that cultivating container dries 2 ~ 3 days.
After above step terminates, the tolerance to cold of Kandelia candel is enhanced, and directly can carry out the experimental demonstration under low temperature environment, be simply referred to as low temperature stress, to observe the impact of cryogenic conditions on Kandelia candel.
Technical scheme core idea of the present invention is: while guarantee autumn eggplant nursery effect, constantly reduce environmental temperature in seedling raising process, progressively improve autumn eggplant to the adaptive capacity of low temperature, improve the tolerance to cold of autumn eggplant with this.
Using beach ooze as culture medium, main two aspects are considered: the first, and ooze close to the condition of autumn eggplant self-sow, and is convenient to cultivate and test; The second, time low temperature stress (zubzero temperature), other type of rearing easily ties thicker ice sheet, especially water planting, and this often exacerbates the gap growing actual natural environment with mangrove forest, has lacked practice significance, hinders the real world applications of this technology.
So-called climatic cabinate be namely can Artificial Control temperature, relative moisture, intensity of illumination factor airtight xegregating unit, also known as controllable environment laboratory.All the other parameters of this phytotron arbitrarily can arrange environment, and the restriction not by natural conditions such as geography, seasons also can shorten the cycle studied.
The technical program is before being adjusted to low temperature environment, promotes that Kandelia candel carries out vigorous photosynthesis by the high temperature close with the external world, produces the nutriment (osmotic adjustment) being conducive to alleviating low temperature stress, as sugar and amino acid.And then by interim gradient cooling, the accumulation of permeate substance can be promoted, increase irreducible water/free water ratio, increase the content of unsaturated fatty acid in film fat, effectively prevent biomembranous phase transformation, strengthen the tolerance to cold of autumn eggplant.In addition, the method is easy to operation, can transplant research provide reliable method and approach for later Kandelia candel mangrove to more high latitude.
Be described in further detail below in conjunction with accompanying drawing and concrete illustration.
Illustration
Test material: autumn eggplant plumular axis, from separating test nearer region obtain, and be divided into three process, be respectively TP, TY, WY:
TP be from plumular axis, carry out cold resistant training until the autumn eggplant grow to eight leaves wholeheartedly;
TY be nursery early stage do not carry out cold resistant training, autumn eggplant plumular axis grow to eight leaves wholeheartedly time carry out cold resistant training;
WY is that whole nursery stage does not all carry out cold resistant training;
Until TP, TY and WY grow to eight leaves wholeheartedly after, carry out the low temperature stress of same time, then under appropriate temperature conditions, carry out same time recovery, carry out photosynthetic parameters measure and collected specimens measure photosynthetic index of correlation.
The prior step of TP, TY, WY is all identical:
The beach of river mouth or littoral zone takes ooze, after the rubble in removing ooze and root system of plant, after natural air drying is broken into pieces, is loaded on and is convenient to carrying and in the watertight cultivating container in bottom, the volume of soil is 2/3 of container volume.Get harmless ripe autumn eggplant plumular axis to insert in container, and unsuitable dense, pouring NaCl concentration is the nutrient solution of 10 ‰, keeps inner bucket water layer 1-2cm.
Wherein, the collocation method of nutrient solution is:
(A), pH value is the calcium nitrate solution of 6.0, concentration 82.07g/L or the four water-calcium nitrate solution of concentration 118.08g/L;
(B), pH value is the Adlerika of the potassium nitrate solution of 8.0, concentration 50.55g/L or the potassium dihydrogen phosphate of concentration 13.61g/L or concentration 24.07g/L or the epsom salt solution of concentration 49.03g/L;
(C), pH value is the Nat-EDTA solution of 5.5, concentration 7.46g/L or the ferrous sulfate heptahydrate solution of concentration 5.56g/L;
(D), the molybdenum acid solution of the white vitriol solution of the BAS of concentration 2.86g/L or four aqueous magnesium chloride solutions of concentration 1.81g/L or concentration 0.22g/L or the cupric sulfate pentahydrate solution of concentration 0.08g/L or concentration 0.02g/L;
Then mixing is settled to 1L, (A), (B), (C), (D) and water is mixed in the ratio of 10ml, 10ml, 1ml, 1ml, 978ml respectively specifically, is pancebrin.
The proportioning of nutrient solution also can be with following methods, to replace the first nutrient solution above-mentioned to use, and the nursery not impact on TP, TY, WY:
Ca (the NO of 1.18g/L
3)
24H
2o solution, the KNO of 0.51g/L
3solution, the MgSO of 0.49g/L
47H
2o solution, the KH of 0.14g/L
2pO
4solution, the H of 2.86mg/L
3bO
3solution, the MnCl of 0.22mg/L
24H
2o solution, the ZnSO of 0.22mg/L
47H
2o solution, the CuSO of 0.08mg/L
45H
2o solution, the H of 0.02mg/L
2moO
4h
2o solution, the FeEDTA solution of 2mL/L.
(1), the test procedure of TP is:
(1), the cultivating container being inserted with autumn eggplant plumular axis put into climatic cabinate, the parameter of adjustment climatic cabinate is: 15 DEG C of (daytime)/10 DEG C (night), relative moisture control to equal 600 μm of olm in 50%-70%, intensity of illumination
-2s
-1, every day, daylight was according to 12h, and cultivate 2 days, the parameter then adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), light application time, intensity and relative moisture are all constant;
(2), when autumn eggplant plumular axis grow two leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 20 DEG C of (daytime)/15 DEG C (night), cultivate 2 days, illumination and relative moisture are all constant,
Then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), and illumination and relative moisture are still all constant;
(3), when autumn eggplant plumular axis grow to four leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture are all constant, cultivate 3 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture are all constant, cultivate 3 days, then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant;
(4), when autumn eggplant plumular axis grow to six leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant;
(5), when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 10 DEG C of (daytime)/5 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 days
Wherein the management process of (1) to (5) each step all: supplement not containing the nutrient solution of NaCl in time, keep water layer 1-2cm, namely the water layer that nutrient solution is formed exceeds soil 1-2cm all the time, but (1) to (5) each step entirety during need to ensure that cultivating container dries 2 ~ 3 days, namely it is the nurturing period of each step, except keeping the date of nutrient solution water layer 1-2cm, need additionally to make cultivating container dry 2 ~ 3 days, be good with 3 days, prevent the long time to be submerged in nutrient solution and injury is produced to autumn eggplant.
(2), the test procedure of TY is:
(1), the cultivating container being inserted with autumn eggplant plumular axis put into climatic cabinate, the parameter of adjustment climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), it is 600 μm of olm that relative moisture controls in 50%-70%, intensity of illumination
-2s
-1, every day, daylight was according to 12h.Period, pouring NaCl concentration is the nutrient solution of 10 ‰, and keep inner bucket water layer 1-2cm, all the other times supplement not containing the nutrient solution of NaCl, and during ensureing often to grow a pair blade, cultivating container dries 2 ~ 3 days.
(2) when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, take the measure identical with the step of TP (5), namely the parameter adjusting climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 10 DEG C of (daytime)/5 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 days.
(3), the test procedure of WY is:
The cultivating container being inserted with autumn eggplant plumular axis is put into climatic cabinate, and the parameter of adjustment climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), relative moisture control to equal 750 μm of olm in 50%-70%, intensity of illumination
-2s
-1, daylight was according to 12h, until grow into eight leaves wholeheartedly every day.Period, pouring NaCl concentration is the nutrient solution of 10 ‰, and keep inner bucket water layer 1-2cm, all the other times supplement not containing the nutrient solution of NaCl, and during ensureing often to grow a pair blade, cultivating container dries 2 ~ 3 days.
Then low temperature stress is carried out respectively by growing to eight leaves TP, TY and WY wholeheartedly, to be placed on temperature be specifically 5 DEG C (48h under the condition in)/– in daytime 2 DEG C (nights), and the intensity of illumination parameters of three process remain original constant.Then (recover 72h under 30 DEG C of (daytime)/25 DEG C (night) conditions carry out photosynthetic parameters mensuration in preference temperature, and collected specimens measures photosynthetic index of correlation.
1 measures project and method
1.1, the observation of leaf color
Record low temperature 48h respectively processes the change of leaf color.
1.2, the mensuration of photosynthetic parameters
Autumn eggplant is recovered 30min under natural lighting condition, measures photosynthetic parameters.The portable photosynthetic instrument of Li-6400 type is used to measure Net Photosynthetic Rate (Pn), stomatal conductance (Gs), the transpiration rate (Tr) of the 3rd pair of blade selecting the number downwards from top in 10:00-11:30.
1.3, the mensuration of Contents of Photosynthetic Pigments
Take 0.1g blade and be cut into fragment, be placed in 25ml volumetric flask, add extract (absolute ethyl alcohol of 1:1 and acetone) constant volume, be placed on dark condition lower 24 hours, then extract is isolated, measure its light absorption value under 470nm, 649nm and 665nm wavelength respectively, chlorophyllous content is according to formulae discovery.
1.4, the assay method of malonaldehyde (MDA) content
Take the examination material 1g shredded, add 2mll0%TCA and a small amount of quartz sand, be ground to homogenate, then add 8mlTCA grinding, homogenate is at the centrifugal 10min of 4000rmin-1, and supernatant is sample extracting solution.Draw centrifugal supernatant 2ml (contrast adds 2ml distilled water), add 2ml0.6%TBA solution, homomixture reacts 15min on boiling water bath, centrifugal again after cooling rapidly.Get the dullness under supernatant mensuration 532,600 and 450nm wavelength.According to formulae discovery below:
MDA content (μm ol/g)=[MDA concentration (μm ol/L) × extracting liquid volume (mL)]/[example weight (g) × 1000].
1.5, data processing
Adopt SPSS21 software to carry out variance analysis and LSD test of significance to experimental data, and use EXCEL2013 to make histogram.
The result obtained after said determination is as follows:
Accompanying drawing explanation
Fig. 1 is the impact of Different treatments on Kandelia candel leaf Net Photosynthetic Rate.
Fig. 2 is the impact of Different treatments on Kandelia candel leaf stomatal conductance.
Fig. 3 is the impact of Different treatments on Kandelia candel leaf transpiration rate.
Fig. 4 is the impact of Different treatments on Kandelia candel leaf chlorophyll content.
Fig. 5 is the impact of Different treatments on Kandelia candel leaf MDA content.
2, results and analysis
2.1 leaf color changes
Under low temperature stress, seedling (TP) blade performed physical exercise from plumular axis is in green, and seedling (TY) blade that performs physical exercise from seedling is light green color, is micro-brown without any exercise seedling (WY) blade.Show that three kinds of process tolerances to cold are followed successively by: TP>TY>WY.
2.2, on the impact of Kandelia candel leaf Net Photosynthetic Rate
With reference to figure 1, when low temperature stress 48h, from plumular axis, carry out the seedling (TP) that processes and from seedling, process 2.0 times and 1.54 times that (TY) net photosynthetic rate is untreated (WY) respectively, compare between three process, all there is significant difference (P<0.05).Recover 72h under preference temperature after, TP and TY process is still significantly higher than WY (P<0.05), and TP process is significantly higher than TY process (P<0.05).
2.3, on the impact of Kandelia candel leaf stomatal conductance
With reference to figure 2, with compared with any process plant, under low temperature stress 48h, TP and TY process improves seedling leaves stomatal conductance, and increases by 87.8% and 35.7% respectively, all there is significant difference (P<0.05).After preference temperature recovers 72h, three kinds for the treatment of conditions lower blade stomatal conductances all increase, but TP and TY process is still significantly higher than without any process plant (WY, P<0.05), and TP process is significantly higher than TY process (P<0.05).
2.4, on the impact of Kandelia candel leaf transpiration rate
With reference to figure 3, during low temperature stress 48h, the lower seedling leaves transpiration rate of TP and TY process is enhanced, and is 1.85 times and 1.33 times without any process plant (WY) respectively, all reaches significant difference (P<0.05).After preference temperature recovers 72h, seedling leaves transpiration rate all increases, but TP and TY process is still significantly higher than without any process plant (P<0.05), and TP process is significantly higher than TY process (P<0.05).
2.5, on the impact of Kandelia candel leaf chlorophyll content
With reference to figure 4, during low temperature stress 48h, TP and TY process improves seedling leaves chlorophyll content, is 1.68 times and 1.28 times without any process plant (WY) respectively, all reaches significant difference (P<0.05).After preference temperature recovers 72h, seedling leaves chlorophyll content all increases, but TP and TY process is still significantly higher than without any process plant (P<0.05), and TP process is significantly higher than TY process (P<0.05).
2.6, on the impact of Kandelia candel leaf MDA content
With reference to figure 5, during low temperature stress 48h, TP and TY process reduces seedling leaves MDA content, decreases 33.91% and 21.78% respectively, all reach significant difference (P<0.05) than without any process plant (WY).After preference temperature recovers 72h, seedling leaves MDA content all reduces, but TP and TY process is still remarkable in without any process plant (P<0.05), and TP process is significantly lower than TY process (P<0.05).
3, conclusion
Net Photosynthetic Rate, stomatal conductance, transpiration rate and chlorophyll content are photosynthetic major parameters, and the larger photosynthetic capacity of its numerical value is stronger.Malonaldehyde (MDA) is one of end-product of Lipid peroxidation metabolism, and its content height can as one of checking cell index being forced the order of severity, and its main injury causes Lipid peroxidation metabolism.From test data, from plumular axis, carry out cold acclimation (TP) and all can improve seedling photosynthetic capacity cryogenic conditions from seedling cold acclimation (TY), reduce low temperature stress to the injury of Lipid peroxidation metabolism, closely strengthen the tolerance to cold of low temperature stress lower autumn eggplant, and the tolerance to cold of carrying out cold acclimation (TP) from plumular axis is higher than from seedling cold acclimation plant (TY), and from the tolerance to cold of seedling cold acclimation plant (TY) higher than without any exercise seedling (WY).
Claims (7)
1. strengthen a method for autumn eggplant cold resistance, comprise following step:
(a), on the beach of river mouth or littoral zone, take ooze, removing ooze in rubble and root system of plant after, after natural air drying is broken into pieces, be loaded on be convenient to carrying and in the watertight cultivating container in bottom, getting harmless ripe autumn eggplant plumular axis inserts in cultivating container, pouring NaCl concentration is the nutrient solution of 10 ‰, keeps inner bucket water layer 1-2cm;
(b), the cultivating container being inserted with autumn eggplant plumular axis is put into climatic cabinate, adjustment climatic cabinate parameter be: temperature 13 ~ 15 DEG C of (daytime)/9 ~ 10 DEG C (night), relative moisture control to be more than or equal to 600 μm of olm in 50%-70%, intensity of illumination
-2s
-1, every day, daylight was according to 11 ~ 13h, cultivated 2 ~ 3 days,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), light application time, intensity and relative moisture are all constant;
(c), when autumn eggplant plumular axis grow two leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), cultivate 2 ~ 3 days, illumination and relative moisture are all constant,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), and illumination and relative moisture are still all constant;
(d), when autumn eggplant plumular axis grow to four leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture are all constant, cultivate 2 ~ 3 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), and illumination and relative moisture are all constant, cultivates 3 ~ 4 days,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), illumination and relative moisture constant;
(e), when autumn eggplant plumular axis grow to six leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 5 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 5 days,
Then the parameter adjusting climatic cabinate is: temperature 28 ~ 30 DEG C of (daytime)/24 ~ 26 DEG C (night), illumination and relative moisture constant;
(f), when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 18 ~ 21 DEG C of (daytime)/13 ~ 16 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 6 days,
Then the parameter adjusting climatic cabinate is: temperature 14 ~ 16 DEG C of (daytime)/9 ~ 10 DEG C (night), illumination and relative moisture constant, cultivate 4 ~ 6 days,
Then the parameter adjusting climatic cabinate is: temperature 9 ~ 11 DEG C of (daytime)/5 ~ 6 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 ~ 6 days,
Wherein the management process of (b), (c), (d), (e), (f) each step all: supplement not containing the nutrient solution of NaCl in time, keep water layer 1-2cm, period also ensures that cultivating container dries 2 ~ 3 days.
Nutrient solution wherein comprises:
(A), pH value is the calcium nitrate solution of 6.0, concentration 82.07g/L or the four water-calcium nitrate solution of concentration 118.08g/L;
(B), pH value is the Adlerika of the potassium nitrate solution of 8.0, concentration 50.55g/L or the potassium dihydrogen phosphate of concentration 13.61g/L or concentration 24.07g/L or the epsom salt solution of concentration 49.03g/L;
(C), pH value is the EDTA-2Na solution of 5.5, concentration 7.46g/L or the ferrous sulfate heptahydrate solution of concentration 5.56g/L;
(D), the molybdenum acid solution of the white vitriol solution of the BAS of concentration 2.86g/L or four aqueous magnesium chloride solutions of concentration 1.81g/L or concentration 0.22g/L or the cupric sulfate pentahydrate solution of concentration 0.08g/L or concentration 0.02g/L;
Then (A), (B), (C), (D) and water are mixed in the ratio of 10ml, 10ml, 1ml, 1ml, 978ml respectively.
2. a kind of method strengthening autumn eggplant cold resistance according to claim 1, is characterized in that:
Described step (b), the cultivating container being inserted with autumn eggplant plumular axis is put into climatic cabinate, the parameter of adjustment climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), relative moisture control 60%, intensity of illumination is more than or equal to 600 μm of olm
-2s
-1, every day, daylight was according to 12h, cultivated 2 days,
Then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), light application time, intensity and relative moisture are all constant.
3. a kind of method strengthening autumn eggplant cold resistance according to claim 1 and 2, is characterized in that:
Described step (c), when autumn eggplant plumular axis grow two leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 20 DEG C of (daytime)/15 DEG C (night), cultivate 2 days, illumination and relative moisture are all constant,
Then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture are still all constant.
4. a kind of method strengthening autumn eggplant cold resistance according to claim 1 and 2, is characterized in that:
Described step (d), when autumn eggplant plumular axis grow to four leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture are all constant, cultivate 3 days,
Then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), and illumination and relative moisture are all constant, cultivates 3 days,
Then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant.
5. a kind of method strengthening autumn eggplant cold resistance according to claim 1 and 2, is characterized in that:
Described step (e), when autumn eggplant plumular axis grow to six leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days,
Then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days,
Then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant.
6. a kind of method strengthening autumn eggplant cold resistance according to claim 1 and 2, is characterized in that:
Described step (f), when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, adjustment climatic cabinate parameter be: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days,
Then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days,
Then the parameter adjusting climatic cabinate is: temperature 10 DEG C of (daytime)/5 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 days.
7. a kind of method strengthening autumn eggplant cold resistance according to claim 1, is characterized in that:
Described step (b), the cultivating container being inserted with autumn eggplant plumular axis is put into climatic cabinate, the parameter of adjustment climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), relative moisture control 60%, intensity of illumination is more than or equal to 600 μm of olm
-2s
-1, every day, daylight was according to 12h, and cultivate 2 days, the parameter then adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), light application time, intensity and relative moisture are all constant;
Described step (c), when autumn eggplant plumular axis grow two leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), cultivate 2 days, illumination and relative moisture are all constant, then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), and illumination and relative moisture are still all constant;
Described step (d), when autumn eggplant plumular axis grow to four leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture are all constant, cultivate 3 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture are all constant, cultivate 3 days, then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant;
Described step (e), when autumn eggplant plumular axis grow to six leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 30 DEG C of (daytime)/25 DEG C (night), illumination and relative moisture constant;
Described step (f), when autumn eggplant plumular axis grow to eight leaves wholeheartedly time, the parameter of adjustment climatic cabinate is: temperature 20 DEG C of (daytime)/15 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 15 DEG C of (daytime)/10 DEG C (night), illumination and relative moisture constant, cultivate 4 days, then the parameter adjusting climatic cabinate is: temperature 10 DEG C of (daytime)/5 DEG C (night), period intensity of illumination and relative moisture all constant, cultivate 4 days.
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| CN106797819A (en) * | 2017-01-05 | 2017-06-06 | 南通大学 | A kind of mangrove seedling fostering system and method to the north of mangrove distributing edge |
| CN107873370A (en) * | 2017-11-28 | 2018-04-06 | 舟山赛莱特海洋科技有限公司 | A kind of mangrove moves northward acclimation method |
| CN107960261A (en) * | 2017-11-28 | 2018-04-27 | 舟山赛莱特海洋科技有限公司 | A kind of potted plant cold-resistant acclimation method of autumn eggplant seedling for high latitude plantation |
| CN112544371A (en) * | 2020-11-30 | 2021-03-26 | 吉林省农业科学院 | Method for identifying low temperature resistance of maize inbred line in germination period |
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| CN104982281A (en) * | 2015-06-10 | 2015-10-21 | 南通安立可农产品科技有限公司 | Planting method of kandelia candel at southern area of 37 degree north latitude |
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| CN112544371B (en) * | 2020-11-30 | 2022-04-15 | 吉林省农业科学院 | Method for identifying low temperature resistance of maize inbred line in germination period |
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