CN105395862B - A kind of application of the pharmaceutical composition containing radix achyranthis bidentatae - Google Patents
A kind of application of the pharmaceutical composition containing radix achyranthis bidentatae Download PDFInfo
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- CN105395862B CN105395862B CN201510824290.6A CN201510824290A CN105395862B CN 105395862 B CN105395862 B CN 105395862B CN 201510824290 A CN201510824290 A CN 201510824290A CN 105395862 B CN105395862 B CN 105395862B
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention discloses a kind of pharmaceutical compositions containing radix achyranthis bidentatae, contain the medicinal materials such as radix achyranthis bidentatae, radix achyranthis bidentatae carries out Processing methods using electric heating constant-temperature blowing drying box, microwave and infrared heating device etc., and discloses application of the pharmaceutical composition containing radix achyranthis bidentatae in preparation treatment rheumatoid arthritis agents.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine preparation, and in particular to a kind of pharmaceutical composition and application containing radix achyranthis bidentatae.
Background technique
The conventional drying process of radix achyranthis bidentatae is sulfur fumigation, and sulfur fumigation is with a long history, drying, the storage etc. to radix achyranthis bidentatae medicinal material
There is significant role, but there is also very big problems for radix achyranthis bidentatae sulfur fumigation, it is not only possible to change the chemical component and then shadow of radix achyranthis bidentatae
It rings and arrives drug effect, and sulfur fumigation can pollute medicinal material, aggravate toxicity, there is certain harm to human body and environment.The prior art
In, there has been no the pharmaceutical compositions containing radix achyranthis bidentatae using microwave, the report of infrared technique in terms of extracting preparation, and water is used to decoct
Method, technique is coarse, falls behind, and impurity is more, causes patient's dosage excessive, it has not been convenient to take, seriously affect this product in clinic
Upper application.
Summary of the invention
Goal of the invention: to solve the above-mentioned problems, the purpose of the present invention is to provide a kind of pharmaceutical compositions containing radix achyranthis bidentatae
And application.
Technical solution: the purpose of the present invention is what is realized by following scheme:
A kind of pharmaceutical composition containing radix achyranthis bidentatae, pharmaceutical composition hang 30 parts of rock wind, hard common calanthe herb 10 by 20 parts of radix achyranthis bidentatae
Part, 20 part, mao vane rings rattan 10g of redback chrismashush root and leaf, 20 parts of Caulis Spatholobi, 50 parts of ramulus cinnamomi, 15 parts of ramulus mori, 20 parts of the root bark of Chinese wolf-berry, 5 parts of Radix Glycyrrhizae works
It is made of bulk pharmaceutical chemicals, preparation method is made of the following steps: under the conditions of taking radix achyranthis bidentatae to be placed in 60 DEG C in electric heating constant-temperature blowing drying box
It is dried to taking-up when medicinal material moisture content is 40%, then is dried to medicinal material moisture content under the conditions of 380V, 20-30mA with microwave and is
It is taken out when 20%, then is placed in drying medicinal material moisture in infrared heating device and is cooled 10% hereinafter, taking out, closed with other medicinal materials
And the water of 10 times of weight of dosing material, 0.5h is impregnated, is decocted 1 hour, filtering, the dregs of a decoction add the water of 8 times of weight of medicinal material, decoct 1
Hour, medical fluid filters out, and merges decoction liquor twice, and being concentrated under reduced pressure into 60 DEG C of relative densities is 1.05, and ethyl alcohol is added to make the body of alcohol content
Product percentage is stirred up to 75%, is stood, filtering, and relative density is 1.25 when filtrate decompression is concentrated into 65 DEG C, and recycles ethyl alcohol,
Concentrate is obtained, concentrate is spray-dried, spray drying condition are as follows: inlet air temperature is 100 DEG C, and leaving air temp is 90 DEG C, material
Temperature is 70 DEG C, and atomizing pressure is 0.2 megapascal, and spray velocity 10ml/s adds auxiliary material and capsule, tablet, granule is made.
The pharmaceutical composition containing radix achyranthis bidentatae, in preparation method infrared heating device be Guangdong beauty micro-wave oven manufacture it is limited
EG238-AH (F) infra-red furnace of company's production.
Application of the pharmaceutical composition containing radix achyranthis bidentatae in preparation treatment rheumatoid arthritis agents.
The utility model has the advantages that Chinese medicine thinks, rheumatic arthritis refers to since the exopathogens such as wind, cold, wet, heat invade human body, impatency
Channels and collaterals, being ached with muscle, muscles and bones, joint, be numb, weighed, joint stuffiness caused by QI-blood circulation is unsmooth, even swollen joint
The big scorching hot disease waited as main clinical manifestation.Radix achyranthis bidentatae of the present invention hangs rock wind, hard common calanthe herb, redback chrismashush root and leaf, hair vane rings rattan
Dredging the channel, activating microcirculation and removing stasis medicinal are monarch drug in a prescription, and Caulis Spatholobi, ramulus cinnamomi, ramulus mori, the root bark of Chinese wolf-berry are clearing heat and detoxicating, reinforcing spleen and kidney is ministerial drug, and Radix Glycyrrhizae is
Adjuvant, the complete mutual compatibility in side, dredging the channel, activating microcirculation and removing stasis medicinal, treatment rheumatic arthritis effect are obvious.
Specific embodiment
Form by the following examples is described in further detail above content of the invention again, but should not be by this
The range for being interpreted as the above-mentioned theme of the present invention is only limitted to example below, and all technologies realized based on above content of the present invention are equal
Belong to the scope of the present invention.
Embodiment 1
Radix achyranthis bidentatae 20g is taken, rock wind 30g, hard common calanthe herb 10g, redback chrismashush root and leaf 20g, hair vane rings rattan 10g, Caulis Spatholobi are hung
20g, ramulus cinnamomi 50g, ramulus mori 15g, root bark of Chinese wolf-berry 20g, Radix Glycyrrhizae 5g, electric heating constant-temperature blowing drying box under the conditions of first taking radix achyranthis bidentatae to be placed in 60 DEG C
In be dried to taking-up when medicinal material moisture content is 40%, then be dried to medicinal material moisture content under the conditions of 380V, 20-30mA with microwave and be
It is taken out when 20%, then is placed in drying medicinal material moisture in infrared heating device and is cooled 10% hereinafter, taking out, closed with other medicinal materials
And the water of 10 times of weight of dosing material, 0.5h is impregnated, is decocted 1 hour, filtering, the dregs of a decoction add the water of 8 times of weight of medicinal material, decoct 1
Hour, medical fluid filters out, and merges decoction liquor twice, and being concentrated under reduced pressure into 60 DEG C of relative densities is 1.05, and ethyl alcohol is added to make the body of alcohol content
Product percentage is stirred up to 75%, is stood, filtering, and relative density is 1.25 when filtrate decompression is concentrated into 65 DEG C, and recycles ethyl alcohol,
Concentrate is obtained, concentrate is spray-dried, spray drying condition are as follows: inlet air temperature is 100 DEG C, and leaving air temp is 90 DEG C, material
Temperature is 70 DEG C, and atomizing pressure is 0.2 megapascal, and spray velocity 10ml/s adds customary adjuvant to be prepared into tablet, every 0.4g.
Infrared heating device is EG238-AH (F) infra-red furnace that the micro-wave oven Manufacturing Co., Ltd of Guangdong beauty produces.
Embodiment 2
Radix achyranthis bidentatae 20g is taken, rock wind 30g, hard common calanthe herb 10g, redback chrismashush root and leaf 20g, hair vane rings rattan 10g, Caulis Spatholobi are hung
20g, ramulus cinnamomi 50g, ramulus mori 15g, root bark of Chinese wolf-berry 20g, Radix Glycyrrhizae 5g, electric heating constant-temperature blowing drying box under the conditions of first taking radix achyranthis bidentatae to be placed in 60 DEG C
In be dried to taking-up when medicinal material moisture content is 40%, then be dried to medicinal material moisture content under the conditions of 380V, 20-30mA with microwave and be
It is taken out when 20%, then is placed in drying medicinal material moisture in infrared heating device and is cooled 10% hereinafter, taking out, closed with other medicinal materials
And the water of 10 times of weight of dosing material, 0.5h is impregnated, is decocted 1 hour, filtering, the dregs of a decoction add the water of 8 times of weight of medicinal material, decoct 1
Hour, medical fluid filters out, and merges decoction liquor twice, and being concentrated under reduced pressure into 60 DEG C of relative densities is 1.05, and ethyl alcohol is added to make the body of alcohol content
Product percentage is stirred up to 75%, is stood, filtering, and relative density is 1.25 when filtrate decompression is concentrated into 65 DEG C, and recycles ethyl alcohol,
Concentrate is obtained, concentrate is spray-dried, spray drying condition are as follows: inlet air temperature is 100 DEG C, and leaving air temp is 90 DEG C, material
Temperature is 70 DEG C, and atomizing pressure is 0.2 megapascal, and spray velocity 10ml/s adds customary adjuvant to be prepared into capsule.Infrared heating
Device is EG238-AH (F) infra-red furnace that the micro-wave oven Manufacturing Co., Ltd of Guangdong beauty produces.
Embodiment 3
Radix achyranthis bidentatae 20g is taken, rock wind 30g, hard common calanthe herb 10g, redback chrismashush root and leaf 20g, hair vane rings rattan 10g, Caulis Spatholobi are hung
20g, ramulus cinnamomi 50g, ramulus mori 15g, root bark of Chinese wolf-berry 20g, Radix Glycyrrhizae 5g, electric heating constant-temperature blowing drying box under the conditions of first taking radix achyranthis bidentatae to be placed in 60 DEG C
In be dried to taking-up when medicinal material moisture content is 40%, then be dried to medicinal material moisture content under the conditions of 380V, 20-30mA with microwave and be
It is taken out when 20%, then is placed in drying medicinal material moisture in infrared heating device and is cooled 10% hereinafter, taking out, closed with other medicinal materials
And the water of 10 times of weight of dosing material, 0.5h is impregnated, is decocted 1 hour, filtering, the dregs of a decoction add the water of 8 times of weight of medicinal material, decoct 1
Hour, medical fluid filters out, and merges decoction liquor twice, and being concentrated under reduced pressure into 60 DEG C of relative densities is 1.05, and ethyl alcohol is added to make the body of alcohol content
Product percentage is stirred up to 75%, is stood, filtering, and relative density is 1.25 when filtrate decompression is concentrated into 65 DEG C, and recycles ethyl alcohol,
Concentrate is obtained, concentrate is spray-dried, spray drying condition are as follows: inlet air temperature is 100 DEG C, and leaving air temp is 90 DEG C, material
Temperature is 70 DEG C, and atomizing pressure is 0.2 megapascal, and spray velocity 10ml/s adds customary adjuvant to be prepared into granule.It is infrared to add
Hot charging is set to EG238-AH (F) infra-red furnace of the micro-wave oven Manufacturing Co., Ltd production of Guangdong beauty.
Hanging rock wind is the different leaf Boston ivy Parthenocissus heterophyllus of Vitaceae Parthenocissus plant
(Blume) Merr. is used as medicine with root, stem.Autumn adopts, and cleans slice, dries.Dispelling, collateral-activating, promoting blood circulation and stopping pain.For rheumatism muscles and bones
Bitterly, leukorrhea with reddish discharge, postpartum abdominal pain；Fracture, treating swelling and pain by traumatic injury, boil are controlled in external application.The present invention is used as medicine with root.
Hard common calanthe herb is orchid family Calanthe plant calanthe Calanthe discolor Lindl., with herb and
Rhizomes is used as medicine.The four seasons can adopt, and dry.Activating microcirculation and removing stasis medicinal, carbuncle that disappears dissipating bind.For scrofula, ulcerative carbuncle pyogenic infections, traumatic injury,
Treating rheumatic ostealgia.The present invention uses all herbal medicine.
Redback chrismashush root and leaf is Euphorbiaceae mountain waste of flax platymiscium red back mountain hemp rod Alchornea trewioides (Benth.)
Muell.-Arg., it is used as medicine with root, leaf.The root four seasons can adopt, and summer and autumn picking leaves is dried.Clearing heat and promoting diuresis, dissipating stasis and stanching bleeding.For dysentery,
Difficult urination, blood urine, lithangiuria is red to collapse, leukorrhea, pain in waist and lower extremities, treating swelling and pain by traumatic injury；Traumatic hemorrhage, nettle rash, eczema are controlled in external application.
The present invention is used as medicine with root.
Hair vane rings rattan is Menispermaceae Cyclea vegetable hair vane rings rattan Cyclea barbata (Wall.) Miers, with
Root is used as medicine.Whole year can adopt, and remove, heat dissipation removing toxic substances, scattered silt analgesic.For acute tonsillitis, sphagitis is had a toothache, stomachache, abdomen
Bitterly, gastroenteritis, malaria, traumatic injury.Other Chinese medicines of the invention are pharmacopeia kind.
Embodiment 4: the pharmacodynamic study of present invention treatment rheumatic arthritis
1 experimental material
1.1 experimental drugs: it plant composition of the present invention containing radix achyranthis bidentatae: is prepared by 1 method of above-described embodiment.
1.2 experimental animals: ICR mouse (cleaning grade) 150, female mice 40, male mouse 110,20 ± 2g of weight.By raising
State University Hospital Correlation Centre provides, animal quality certification number: NO.201501647.Experimental mouse enters behind laboratory according to experiment
Scheme divides cage, raises 3 days to adapt to laboratory environment, freely ingests, drinks water, and 22~25 DEG C of raising temperature, humidity 60%, feed
It is provided by Jiangning county Qinglongshan animal reproduction field.In experimentation, animal ethics standard is met to the disposition of animal.
1.3 experiment reagents: physiological saline (it is produced by Jiangsu Huaian Co., Ltd of Double-Crane Pharmaceutical Co., Ltd, product batch number are as follows:
140814).Dimethylbenzene (is produced, product batch number are as follows: 20150116) by Guangdong Guanghua Science and Technology Co., Ltd..0.01% ground plug
The loose suspension of rice: dexamethasone acetate tablets (are provided, product batch number are as follows: 015140803) by Shanghai Sine Pharmaceutical Co., Ltd.
It grinds, weighs 10mg medicinal powder, add distilled water to 100ml constant volume to get dexamethasone suspension.2% aspirin suspension: will
Aspirin enteric coated tablet (being provided by Bayer HealthCare Co, product batch number are as follows: BJ18018) is ground, and weighs 1g medicinal powder,
Add distilled water to 50ml constant volume to get aspirin suspension.1.2% glacial acetic acid: precision measures glacial acetic acid (by Guangdong brilliance section
The production of skill limited liability company, product batch number are as follows: 20141120) 1.2ml adds distilled water to 100ml to get glacial acetic acid solution.
And 0.5% (yellow is provided 5% picric acid by Sinopharm Chemical Reagent Co., Ltd., product batch number are as follows: 71067250)
Acid fuchsin (it is red, it is provided by Sinopharm Chemical Reagent Co., Ltd., product batch number are as follows: 71019360), with two kinds of differences
The reagent of color marks mouse.
1.4 experiment equipments: TD electronic balance (e=10d, d=0.1g, the production of Yuyao City Jin Nuo balance Co., Ltd, production
Lot number are as follows: 2015b), (e=0.001g, d=0.0001g, upper current chart level instruments and meters Co., Ltd are raw for FA1104 electronic balance
Produce, product batch number are as follows: 13D101-1000), (the upper macro experimental facilities Co., Ltd of Nereid is raw for DHG-9140A electric drying oven with forced convection
Produce), punch (9mm), stopwatch etc..
2 experimental methods and result
2.1 mouse acetic acid twistings experiment --- analgesia
ICR mouse 60 are taken, weight 18-22g, half male and half female is randomly divided into 5 groups.Respectively, 1. blank group:
NS10ml/kg, 2. positive controls (aspirin group) dosage 0.2g/kg, 3. low dose group 2g/kg of the present invention, 4. of the invention
Middle dose group 4g/kg, 5. high dose group 8g/kg of the present invention.Each group pressed gastric infusion 7 days of 10ml/kg, in the 7th day last
After 45min is administered, 1.2% glacial acetic acid of intraperitoneal injection (newly matches) 0.2ml/ only.Mouse web portion indent, trunk and four limbs are extended back,
The behavior reactions such as hips up are known as writhing response, i.e., in this, as pain index, record: 1. for the first time the writhing response time (if
Do not occur in 15min, then in terms of 15min) writhing response number of animals that 2. 15min mouse occurs and writhing response number, and it counts
It calculates writhing response and inhibits percentage [(blank control group writhing mean-administration group writhing mean)/blank control group writhing mean] *
100%.SPSS software is finally used, t is examined, and compares the conspicuousness of the difference between administration group and blank control group.Analgesic effect
It is shown in Table 1.
1 present invention of table causes the comparison (X ± S) of mice pain reaction to acetic acid
Note: * * p < 0.01, * p < 0.05 is compared with blank control group
As seen from Table 1, the writhing response number of aspirin group and blank control group has significant difference, illustrates positive drug
Really effectively, animal model success.The writhing response number of each dosage group of the present invention and blank control group has significant difference, can
See that analgesic activity of the present invention is obvious.
The experiment of 2.2 mouse dimethylbenzene ear expandings --- it is anti-inflammatory
Take male ICR mouse, 27-29g of weight.Divide 5 groups at random by weight.Successively are as follows: 1. blank group: physiological saline
10ml/kg, 2. positive controls (dexamethasone tablet) dosage 1mg/kg, 3. low dose group 2g/kg of the present invention, 4. agent in the present invention
Amount group 4g/kg, 5. high dose group 8g/kg of the present invention.Each group pressed gastric infusion 7 days of 10ml/kg, in the 7th day last dose
After 45min, dimethylbenzene 40ul is taken uniformly to be applied to the left auricular concha two sides of mouse.Cervical dislocation puts to death mouse after causing inflammation 1h, along auricle base
Line cuts two ears of left and right, removes round auricle, electronic balance precise weighing, meter at same position respectively with punch (diameter 9mm)
Auricular concha weight is recorded, each group mouse ear swelling degree (left auricular concha weight-auris dextra shell weight) average value is calculated and ear swelling inhibits
Percentage [(blank control group swelling mean-administration group swelling mean)/blank control group swelling mean] * 100%.
Using SPSS16.0 software, t is examined, and compares the conspicuousness of the difference between administration group and blank control group.Antiphlogistic effects are shown in Table
2。
The paraxylene of the present invention of table 2 causes the comparison (X ± S) of mouse ear swelling reaction
Note: * * p < 0.01, * p < 0.01, compared with blank control group.
As can be seen from Table 2, the mouse ear swelling degree of Dexamethasone group and blank control group has significant difference, illustrate positive drug
Really effectively, animal model success.The ear swelling of each dosage group of the present invention and blank control group is substantially reduced, and illustrates the present invention
There is anti-inflammatory effect.
Claims (2)
1. a kind of pharmaceutical composition containing radix achyranthis bidentatae is preparing the application in anti-inflammation analgesis medicament, which is characterized in that the medicine containing radix achyranthis bidentatae
Compositions hang 30 parts of rock wind, 10 parts of hard common calanthe herb, 20 part, mao vane rings rattan 10g of redback chrismashush root and leaf, chicken blood by 20 parts of radix achyranthis bidentatae
20 parts of rattan, 50 parts of ramulus cinnamomi, 15 parts of ramulus mori, 20 parts of the root bark of Chinese wolf-berry, 5 parts of Radix Glycyrrhizae conduct bulk pharmaceutical chemicals are made, and preparation method is by the following steps
Composition: being dried to taking-up when medicinal material moisture content is 40% in electric heating constant-temperature blowing drying box under the conditions of taking radix achyranthis bidentatae to be placed in 60 DEG C, then
With microwave taking-up when being dried to medicinal material moisture content under the conditions of 380V, 20-30mA and being 20%, then it is placed in infrared heating device and dries
Dry medicinal material moisture is cooled 10% hereinafter, taking out, and is merged with other medicinal materials, the water of 10 times of weight of dosing material, and 0.5h is impregnated, and is decocted
1 hour, filtering, the dregs of a decoction added the water of 8 times of weight of medicinal material, decocted 1 hour, and medical fluid filters out, and merged decoction liquor twice, depressurized dense
Being reduced to 60 DEG C of relative densities is 1.05, adds ethyl alcohol to make the percent by volume of alcohol content up to 75%, stirs, stand, filtering, filtrate subtracts
Relative density is 1.25 when pressure is concentrated into 65 DEG C, and recycles ethyl alcohol, obtains concentrate, concentrate is spray-dried, and is spray-dried item
Part are as follows: inlet air temperature is 100 DEG C, and leaving air temp is 90 DEG C, and temperature of charge is 70 DEG C, and atomizing pressure is 0.2 megapascal, spray velocity
For 10ml/s, adds auxiliary material and capsule, tablet, granule is made.
2. the pharmaceutical composition containing radix achyranthis bidentatae is preparing the application in anti-inflammation analgesis medicament as described in claim 1, it is characterised in that
Infrared heating device is that the micro-wave oven Manufacturing Co., Ltd of Guangdong beauty produces in pharmaceutical composition preparation method containing radix achyranthis bidentatae
EG238-AH (F) infra-red furnace.
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