CN105395836A - Application of traditional Chinese medicine composition to preparation of medicine for treating wind-warm lung-heat disease - Google Patents

Application of traditional Chinese medicine composition to preparation of medicine for treating wind-warm lung-heat disease Download PDF

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CN105395836A
CN105395836A CN201511003652.1A CN201511003652A CN105395836A CN 105395836 A CN105395836 A CN 105395836A CN 201511003652 A CN201511003652 A CN 201511003652A CN 105395836 A CN105395836 A CN 105395836A
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parts
radix
chinese medicine
medicine composition
lung
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刘学键
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Qingdao Huazhicao Medical Technology Co Ltd
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Qingdao Huazhicao Medical Technology Co Ltd
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8968Ophiopogon (Lilyturf)
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56Materials from animals other than mammals
    • A61K35/618Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/19Acanthaceae (Acanthus family)
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    • A61K36/23Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/31Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A61K36/315Isatis, e.g. Dyer's woad
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/34Campanulaceae (Bellflower family)
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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    • A61K36/34Campanulaceae (Bellflower family)
    • A61K36/342Adenophora
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    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484Glycyrrhiza (licorice)
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    • A61K36/53Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/539Scutellaria (skullcap)
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    • A61K36/18Magnoliophyta (angiosperms)
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    • A61K36/57Magnoliaceae (Magnolia family)
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    • A61K36/60Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605Morus (mulberry)
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    • A61K36/70Polygonaceae (Buckwheat family), e.g. spineflower or dock
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
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    • A61K36/708Rheum (rhubarb)
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/80Scrophulariaceae (Figwort family)
    • A61K36/804Rehmannia
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    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/85Verbenaceae (Verbena family)
    • A61K36/855Clerodendrum, e.g. glorybower
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8964Anemarrhena
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    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/51Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Abstract

The invention discloses application of a traditional Chinese medicine composition to preparation of medicine for treating wind-warm lung-heat disease and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition is prepared from folium mori, rhizoma anemarrhenae, folium isatidis, scutellaria baicalensis, rheum officinale, straight ladybell roots, dried scallop, commelina communis, ophiopogon roots, Chinese magnoliavine fruits, prepared rehmannia roots and licorice roots. According to the traditional Chinese medicine composition, natural traditional Chinese medicine is adopted, the composition is simple in component and reasonable in matching and has the effects of clearing away the lung-heat, relieving exterior syndromes, ventilating the lungs, resolving phlegm, strengthening body resistance and inhibiting pathogenic factors, the cure rate of treating wind-warm lung-heat disease is extremely high, safety is achieved, no toxic or side effect exists, and good clinic application and popularization prospect is achieved.

Description

The purposes of a kind of Chinese medicine composition in preparation treatment wind-warm lung-heat medicine
Technical field
The invention belongs to technical field of Chinese medicines, particularly relate to the purposes of a kind of Chinese medicine composition in preparation treatment wind-warm lung-heat medicine.
Background technology
Wind-warm lung-heat disease is being collectively referred to as of pyretic pulmonary disease and wind-warm syndrome, be generate heat, cough, exogenous diseases that chest pain etc. is main clinical manifestation.The visible high fever of sick severe one, Blushing, dysphoria, the diseases such as the quiet language of coma or extreme cold of the limbs.Winter and spring is more.Have the advantages that onset is anxious, the change of disease is fast, the course of disease is short.This disease belongs to tcm internal medicine exogenous disease research field, is commonly encountered diseases and the frequently-occurring disease of tcm internal medicine.The acute pneumonia of western medicine, the acute pulmonary infection illness such as peribronchitis and acute bronchitis, all can refer to primary disease determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs.
Current western medicine there is no specific treatment method clinically, still mainly adopt second and third cephalosporin in generation, beta-lactam class, fluoroquinolones is treated, though there is certain curative effect, along with the transition of pathogenic microorganism, drug resistance phenomenon is on the rise, and side effect is comparatively large, some drugs is expensive, therefore is difficult to popularity.
The traditional Chinese medical science thinks that primary disease is because of the positive QI-insufficiency of body, nutrient QI failing to keep internally, and outside cannot not defending imperially, resistance against diseases is low, cruelly feels the heresy of wind heat and sends out.Its route of infection enters from mouth and nose, first violates upper and middle portions of the body cavity lung and defend, stand in the breach, " lung governing qi belong to defend ", so wind-heat invading the lung, outer and heresy just disaccords, interior and lung qi is unclear.Pathological changes emphasis is all the time at lung.
Chinese patent application 201410821866.9 relates to a kind of pharmaceutical composition for the treatment of wind-warm lung-heat disease, and this pharmaceutical composition comprises the Chinese crude drug of following parts by weight: Radix Isatidis, the Rhizoma Anemarrhenae, Artemisia annua L., Fructus Forsythiae, Cortex Mori, Bombyx Batryticatus, Rhizoma Coptidis, Fructus Crataegi, Rhizoma Phragmitis and Radix Glycyrrhizae.Clinically to the treatment of this disease, generally based on the traditional Chinese medical science, but most of prescription drug effect does not show, and the therapeutic effect for wind-warm lung-heat disease is not remarkable.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Chinese medicine composition for the treatment of wind-warm lung-heat disease, and this Chinese medicine composition has clearing away heat and nourishing YIN, promotes the production of body fluid and moisturizes, and the effects such as promoting diuresis to eliminate damp pathogen also have good effect, the advantages such as side effect is low, taking convenience.In addition, the present invention also provides corresponding manufacturing process to be made and facilitate easy-to-use preparation under the prerequisite ensureing curative effect by this Chinese medicine composition.
The object of the invention is to be achieved through the following technical solutions:
The purposes of Chinese medicine composition in preparation treatment wind-warm lung-heat medicine, described Chinese medicine composition is made up of the crude drug of following parts by weight: Folium Mori 15-30 part, Rhizoma Anemarrhenae 10-25 part, Folium Isatidis 9-20 part, Radix Scutellariae 9-15 part, Radix Et Rhizoma Rhei 8-12 part, Radix Adenophorae (Radix Glehniae) 8-12 part, dry scallop 6-12 part, Herba Murdanniae Divergentis 6-12 part, Radix Ophiopogonis 3-10 part, Fructus Schisandrae Chinensis 3-10 part, Radix Rehmanniae Preparata 3-8 part and Radix Glycyrrhizae 1-10 part.
Preferably, described Chinese medicine composition is made up of the crude drug of following parts by weight: 15 parts, Folium Mori, the Rhizoma Anemarrhenae 10 parts, Folium Isatidis 9 parts, Radix Scutellariae 9 parts, Radix Et Rhizoma Rhei 8 parts, Radix Adenophorae (Radix Glehniae) 8 parts, 6 parts, dry scallop, Herba Murdanniae Divergentis 6 parts, Radix Ophiopogonis 3 parts, Fructus Schisandrae Chinensis 3 parts, Radix Rehmanniae Preparata 3 parts and 1 part, Radix Glycyrrhizae.
Preferably, described Chinese medicine composition is made up of the crude drug of following parts by weight: 25 parts, Folium Mori, the Rhizoma Anemarrhenae 20 parts, Folium Isatidis 15 parts, Radix Scutellariae 12 parts, Radix Et Rhizoma Rhei 10 parts, Radix Adenophorae (Radix Glehniae) 10 parts, 9 parts, dry scallop, Herba Murdanniae Divergentis 9 parts, Radix Ophiopogonis 6 parts, Fructus Schisandrae Chinensis 6 parts, Radix Rehmanniae Preparata 5 parts and 5 parts, Radix Glycyrrhizae.
Preferably, described Chinese medicine composition is made up of the crude drug of following parts by weight: 30 parts, Folium Mori, the Rhizoma Anemarrhenae 25 parts, Folium Isatidis 20 parts, Radix Scutellariae 15 parts, Radix Et Rhizoma Rhei 12 parts, Radix Adenophorae (Radix Glehniae) 12 parts, 12 parts, dry scallop, Herba Murdanniae Divergentis 12 parts, Radix Ophiopogonis 10 parts, Fructus Schisandrae Chinensis 10 parts, Radix Rehmanniae Preparata 8 parts and 10 parts, Radix Glycyrrhizae.
Correspondingly, present invention also offers a kind of preparation method of described Chinese medicine composition, comprise the following steps:
(1): get Folium Mori, impurity is washed away, 50 DEG C of hot air dryings 5 hours, control moisture is 3-8%, pulverizes, and crosses 40-60 mesh sieve, obtain coarse powder, coarse powder is sucked air-stream type ultrafine pulverizer and carry out micronizing, pulverize pressure 0.5MPa, charging rate is 30mg/s, micronizing is carried out respectively after drying, cross 800 mesh sieves, obtain Ultra-fine Powder of Mulberry Leaves, for subsequent use;
(2): get the Rhizoma Anemarrhenae, Folium Isatidis, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Adenophorae (Radix Glehniae), dry scallop, Herba Murdanniae Divergentis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, Radix Rehmanniae Preparata and Radix Glycyrrhizae, add the distilled water immersion 2-5 hour of medical material total amount 3-8 times weight, boil 1-3 hour, filter, obtain filtrate and filtering residue, in filtering residue, add the purified water of initial medical material 3-5 times weight, boil 1-2 hour, filter and obtain filtrate and preserve filtering residue, merge twice filtrate, concentrated, obtaining detecting relative density at 65 DEG C is the extractum A of 1.10-1.20, for subsequent use;
(3): the ethanol adding the concentration 60-75% of 3-6 times of weight in the filtering residue of (2), reflux, extract, 1-2 time, each 3-6 hour, filters, merging filtrate, and distilling under reduced pressure removing ethanol is also concentrated, obtains the extractum B that 65 DEG C of relative densities are 1.10-1.20, for subsequent use;
(4): described extractum A mixed homogeneously with described extractum B, drying under reduced pressure, pulverizes, and crosses 100-200 mesh sieve, mixes with Ultra-fine Powder of Mulberry Leaves, adds adjuvant, utilizes modern general Chinese medicine preparation technology to make the finished product preparation of clinical needs.
Preferably, described Chinese medicine composition is made into capsule, tablet, powder or decoction.
Source, the nature and flavor of Chinese prescription of the present invention Chinese crude drug used, return through and effect:
Folium Mori: this product is the dried leaves of moraceae plants Mulberry; Nature and flavor are sweet, bitter, cold; Return lung, Liver Channel; Dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, liver heat removing and eyesight improving.
The Rhizoma Anemarrhenae: this product is the dry rhizome of the liliaceous plant Rhizoma Anemarrhenae; Its bitter in the mouth, sweet, cold in nature; Return lung, stomach, kidney channel; Clearing away heat-fire, promotes the production of body fluid and moisturizes.
Folium Isatidis: this product is the dried leaves of cruciferae isatis; Bitter in the mouth, cold in nature; GUIXIN, stomach warp; Heat-clearing and toxic substances removing, blood cooling and ecchymoses removing.
Radix Scutellariae: this product is the dry root of labiate Radix Scutellariae; Bitter in the mouth, cold in nature; Return lung, gallbladder, spleen, large intestine, small intestine meridian; Heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive.
Radix Et Rhizoma Rhei: this product is the rhizome of polygonum rheum palmatum, Rheum tanguticum or Rheum officinale; Bitter in the mouth, cold in nature; Return spleen, stomach, large intestine, liver, pericardium channel; Purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation.
Radix Adenophorae (Radix Glehniae): this product is campanulaceae Lady bell plant four leaf sand, Folium Pruni Radix Adenophorae (Radix Glehniae) or its congener, is used as medicine with root; Sweet in the mouth, cool in nature; Return lung; Stomach warp; Clearing away heat and nourishing YIN, nourishing the lung to arrest cough.
Dry scallop: this product is the closed shell flesh of Pectenidae animal Chlamys farreri, Chlamys nobilis and flower magpie Chlamys farreri; Sweet in the mouth, salty, slightly warm in nature; Return kidney, spleen channel; YIN nourishing; Nourish blood; The kidney invigorating; In tune.
Herba Murdanniae Divergentis: this product is root or the Herb of Herba Commelinae kind of plant purple back of the body Herba Pyrolae; Sweet in the mouth, micro-hardship, property is put down; GUIXIN, all warps of liver,spleen,kidney, intestine and small intestine; Clearing away lung-heat to relieve cough, lung kidney tonifying, regulating menstruation, hemostasis.
Radix Ophiopogonis: this product is the tuber of liliaceous plant dwarf lilyturf; Sweet in the mouth, property is put down; Return lung, stomach, heart channel; Nourishing YIN and moistening the lung, clear away heart-fire relieving restlessness, reinforcing stomach reg fluid.
Fructus Schisandrae Chinensis: this product is the dry mature fruit of magnoliaceae schisandra or schisandra chinensis; Its sour in the mouth, sweet, warm in nature; Return lung, the heart, kidney channel; Restrain astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, kidney calming.
Radix Rehmanniae Preparata: this product is the Preparation process product of Radix Rehmanniae; Sweet in the mouth, slightly warm in nature; Return liver, kidney channel; Nourishing YIN and supplementing blood, beneficial essence fills out marrow.
Radix Glycyrrhizae: this product is the dry root of glycyrrhizic legume, Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L.; Sweet in the mouth, property is put down; GUIXIN, lung, spleen, stomach warp; Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, relieving spasm to stop pain, coordinating the actions of various ingredients in a prescription.
The compatibility analysis of Chinese prescription of the present invention:
Chinese prescription of the present invention to the dialectical cognition of eczema, follows the compatibility theory of Chinese medicine according to the traditional Chinese medical science, facilitating the easy-to-use and preparation of stable curative effect, enhancing the use amount of medical material to make.This prescription is that heat-clearing and toxic substances removing, promotes the production of body fluid and moisturize with Folium Mori and the Rhizoma Anemarrhenae for monarch drug; With Folium Isatidis, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Adenophorae (Radix Glehniae), dry scallop for ministerial drug, tonifying kidney and nourishing yin, nourishing the lung to arrest cough; With Herba Murdanniae Divergentis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, Radix Rehmanniae Preparata for adjuvant drug, marrow is filled out in the relieving restlessness that clears away heart-fire, beneficial essence; With Radix Glycyrrhizae for making medicine, the coordinating the actions of various ingredients in a prescription property of medicine, all medicines coordinate, and complement each other, synergism, plays clearing away heat and nourishing YIN altogether, promotes the production of body fluid and moisturizes, the effects such as promoting diuresis to eliminate damp pathogen, has very significant curative effect to eczema.
Compared with prior art, beneficial effect of the present invention is summarized as follows:
(1) Chinese medicine composition of the present invention has that lung heat clearing is induced sweat, effect of Fuzheng Yiliu heresy, and not only lung heat clearing is induced sweat, and receives the merit that eliminating pathogenic factor for supporting vital QI consolidates, and has transferred the resistance against diseases of body inherence.Results of animal proves, the high and safe without toxic side effect for the treatment of wind-warm lung-heat disease cure rate.
(2) Chinese medicine composition of the present invention selects high-quality Chinese crude drug to make, and selected medical material compatibility is suitable, meets Chinese medicine and modern medicine study theory, reaches the object for the treatment of both the principal and secondary aspects of a disease, have good potential applicability in clinical practice.
Detailed description of the invention
Below will the present invention is described further by specific embodiment, it will be understood by those skilled in the art that the present invention also can make other similar changes and obtains similar result and do not depart from content of the present invention and scope except following examples.
embodiment 1
The embodiment of the present invention 1 Chinese medicine composition is made up of the crude drug of following parts by weight: 15 parts, Folium Mori, the Rhizoma Anemarrhenae 10 parts, Folium Isatidis 9 parts, Radix Scutellariae 9 parts, Radix Et Rhizoma Rhei 8 parts, Radix Adenophorae (Radix Glehniae) 8 parts, 6 parts, dry scallop, Herba Murdanniae Divergentis 6 parts, Radix Ophiopogonis 3 parts, Fructus Schisandrae Chinensis 3 parts, Radix Rehmanniae Preparata 3 parts and 1 part, Radix Glycyrrhizae.
Preparation method:
(1): get Folium Mori, impurity is washed away, 50 DEG C of hot air dryings 5 hours, controlling moisture is 3%, pulverizes, and crosses 40 mesh sieves, obtain coarse powder, coarse powder is sucked air-stream type ultrafine pulverizer and carry out micronizing, pulverize pressure 0.5MPa, charging rate is 30mg/s, micronizing is carried out respectively after drying, cross 800 mesh sieves, obtain Ultra-fine Powder of Mulberry Leaves, for subsequent use;
(2): get the Rhizoma Anemarrhenae, Folium Isatidis, Radix Scutellariae, Radix Et Rhizoma Rhei, Radix Adenophorae (Radix Glehniae), dry scallop, Herba Murdanniae Divergentis, Radix Ophiopogonis, Fructus Schisandrae Chinensis, Radix Rehmanniae Preparata and Radix Glycyrrhizae, add the distilled water immersion 4 hours of medical material total amount 5 times of weight, boil 2 hours, filter, obtain filtrate and filtering residue, in filtering residue, add the purified water of initial medical material 4 times of weight, boil 1.5 hours, filter and obtain filtrate and preserve filtering residue, merge twice filtrate, concentrated, obtaining detecting relative density at 65 DEG C is the extractum A of 1.15, for subsequent use;
(3): the ethanol adding the concentration 75% of 4 times of weight in the filtering residue of (2), reflux, extract, 1 time, each 4 hours, filters, merging filtrate, and distilling under reduced pressure removing ethanol is also concentrated, obtains the extractum B that 65 DEG C of relative densities are 1.15, for subsequent use;
(4): described extractum A mixed homogeneously with described extractum B, drying under reduced pressure, pulverizes, and crosses 100 mesh sieves, mixes with Ultra-fine Powder of Mulberry Leaves, adds adjuvant, utilizes modern general Chinese medicine preparation technology to make the capsule of clinical needs.
embodiment 2
The embodiment of the present invention 2 Chinese medicine composition is made up of the crude drug of following parts by weight: 25 parts, Folium Mori, the Rhizoma Anemarrhenae 20 parts, Folium Isatidis 15 parts, Radix Scutellariae 12 parts, Radix Et Rhizoma Rhei 10 parts, Radix Adenophorae (Radix Glehniae) 10 parts, 9 parts, dry scallop, Herba Murdanniae Divergentis 9 parts, Radix Ophiopogonis 6 parts, Fructus Schisandrae Chinensis 6 parts, Radix Rehmanniae Preparata 5 parts and 5 parts, Radix Glycyrrhizae.
Preparation method is as embodiment 1.
embodiment 3
The embodiment of the present invention 3 Chinese medicine composition is made up of the crude drug of following parts by weight: 30 parts, Folium Mori, the Rhizoma Anemarrhenae 25 parts, Folium Isatidis 20 parts, Radix Scutellariae 15 parts, Radix Et Rhizoma Rhei 12 parts, Radix Adenophorae (Radix Glehniae) 12 parts, 12 parts, dry scallop, Herba Murdanniae Divergentis 12 parts, Radix Ophiopogonis 10 parts, Fructus Schisandrae Chinensis 10 parts, Radix Rehmanniae Preparata 8 parts and 10 parts, Radix Glycyrrhizae.
Preparation method is as embodiment 1.
chinese medicine composition pharmacodynamic study of the present invention:
test example: the experimentation that Chinese medicine composition of the present invention affects wind-warm lung-heat disease animal model
1, experiment purpose and method
Experiment purpose: according to the requirement of " new Chinese medicine pharmaceutical research guide ", studies the Pharmacodynamics of pharmaceutical composition of the present invention at treatment wind-warm lung-heat disease.Take the embodiment of the present invention 1 to 3 gained pharmaceutical composition, by lung tissue's morphological observation and inflammatory cell count, probe into its high, medium and low dosage to the impact of wind-warm lung-heat disease rat model.
Experimental technique: (Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center provides to choose 120 SPF level SD rats; Body weight is 175-225g).Random selecting is 110 rats wherein, are placed in by rat in special 1m × 1m × 1m smoke-box, with wood shavings, sawdust, each 50g of Nicotiana tabacum L., light fumigation, 30min/ time, 2/d, continue 30d, set up wind-warm lung-heat disease animal model.Other 10 mices are Normal group.
The wind-warm lung-heat disease rat model established is divided into 11 groups at random, is respectively the high, medium and low dosage group of embodiment 1, the high, medium and low dosage group of embodiment 2, the high, medium and low dosage group of embodiment 3, positive drug group and model group, often organize 10.Embodiment of the present invention 1-3 capsule is got capsule shells, gets content and to add water furnishing pasty state; Positive drug group gives Cefixime Capsules, and (Tianjin Medicine Group Jinkang Pharmaceutical Co., Ltd produces; The accurate word H20060932 of traditional Chinese medicines), each group dosage is as shown in table 1 below, and model group and Normal group give the process of isometric(al) normal saline, above-mentioned each group every day gavage 2 times, continuous use 45 days.
Medication, after 45 days, is got in the formalin of left lung 10% fixing, Gradient elution using ethanol, dimethylbenzene transparent specimen, waxdip, embedding, section, HE dyeing, tissues observed morphological change under optical microscope.Get the optical microphotograph Microscopic observation that each sample slice is placed in 400 times, randomly draw 5 visuals field, use Image-ProPlus6.0 software manually to carry out lung tissue inflammatory cell count.
With the chloral hydrate anesthesia mice that 5% dosage is 0.1mL/10g, skin is cut in trachea front, and blunt separation exposes gas
Pipe, the capable bronchoalveolar lavage of phosphate buffer 1 .5mL is used (to divide 3 times, each 0.5mL), reclaim bronchoalveolar lavage fluid about 1ml altogether, 4 DEG C of centrifugal 10min, 1500r/min, reclaim supernatant-20 DEG C preservation, contain the PBS re-suspended cell precipitation of 1%BSA with 1mL, get 10 μ l re-suspension liquid and carry out numeration of leukocyte in blood cell counting plate.Collection of specimens, at duration of test, is weighed in weekly once.
Statistical procedures test data all adopts mean ± standard deviation to represent, group difference significance t inspection is compared, and P<0.05 is for there being significant difference.SPSS19.0 statistical software is adopted to carry out date processing.
2, experimental result is as shown in table 1.
Table 1 Chinese medicine composition of the present invention is on the impact of bronchoalveolar lavage fluid in rats leukocyte and lung tissue inflammatory cell
Note: △ △ compare with Normal group, p<0.01; compare with model group, p<0.05, ▲ ▲compare with model group, p<0.01; #compare with positive drug group, p<0.05, ##compare with positive drug group, p<0.01.
3, conclusion
This experiment judges the curative effect of pharmaceutical composition of the present invention in wind-warm lung-heat disease by the impact of the research high, medium and low dosage of embodiment of the present invention 1-3 gained pharmaceutical composition on bronchoalveolar lavage fluid in rats leukocyte and lung tissue inflammatory cell.First, this experiment makes experimental animal with 120 rats, administration in continuous 45 days, and none is dead for rat, and put to death each internal organs of rat anatomic observation and do not find toxic reaction, rat body weight is also without significant change.Administration group and control group mice are without any difference.Embodiment 1 to 3 high, medium and low dosage group leukocyte count meansigma methods compares with model group statistical significance.
In embodiment 1-3, the lung tissue inflammatory cell number of the high, medium and low dosage group of gained pharmaceutical composition is compared with model group, and each group significantly can be reduced lung tissue inflammatory cell number, and wherein high dose group almost reaches levels found in normal controls.Positive drug group treatment level is far below Chinese medicine composition low dose group of the present invention.Visible, pharmaceutical composition of the present invention can effectively suppress pneumonia to occur, especially better with embodiment 2 effect, has the effect of significant treatment wind-warm lung-heat disease.

Claims (5)

1. the purposes of a Chinese medicine composition in preparation treatment wind-warm lung-heat medicine, it is characterized in that, described Chinese medicine composition is made up of the crude drug of following parts by weight: Folium Mori 15-30 part, Rhizoma Anemarrhenae 10-25 part, Folium Isatidis 9-20 part, Radix Scutellariae 9-15 part, Radix Et Rhizoma Rhei 8-12 part, Radix Adenophorae (Radix Glehniae) 8-12 part, dry scallop 6-12 part, Herba Murdanniae Divergentis 6-12 part, Radix Ophiopogonis 3-10 part, Fructus Schisandrae Chinensis 3-10 part, Radix Rehmanniae Preparata 3-8 part and Radix Glycyrrhizae 1-10 part.
2. the purposes of Chinese medicine composition as claimed in claim 1 in preparation treatment wind-warm lung-heat medicine, it is characterized in that, described Chinese medicine composition is made up of the crude drug of following parts by weight: 15 parts, Folium Mori, the Rhizoma Anemarrhenae 10 parts, Folium Isatidis 9 parts, Radix Scutellariae 9 parts, Radix Et Rhizoma Rhei 8 parts, Radix Adenophorae (Radix Glehniae) 8 parts, 6 parts, dry scallop, Herba Murdanniae Divergentis 6 parts, Radix Ophiopogonis 3 parts, Fructus Schisandrae Chinensis 3 parts, Radix Rehmanniae Preparata 3 parts and 1 part, Radix Glycyrrhizae.
3. the purposes of Chinese medicine composition as claimed in claim 1 in preparation treatment wind-warm lung-heat medicine, it is characterized in that, described Chinese medicine composition is made up of the crude drug of following parts by weight: 25 parts, Folium Mori, the Rhizoma Anemarrhenae 20 parts, Folium Isatidis 15 parts, Radix Scutellariae 12 parts, Radix Et Rhizoma Rhei 10 parts, Radix Adenophorae (Radix Glehniae) 10 parts, 9 parts, dry scallop, Herba Murdanniae Divergentis 9 parts, Radix Ophiopogonis 6 parts, Fructus Schisandrae Chinensis 6 parts, Radix Rehmanniae Preparata 5 parts and 5 parts, Radix Glycyrrhizae.
4. the purposes of Chinese medicine composition as claimed in claim 1 in preparation treatment wind-warm lung-heat medicine, it is characterized in that, described Chinese medicine composition is made up of the crude drug of following parts by weight: 30 parts, Folium Mori, the Rhizoma Anemarrhenae 25 parts, Folium Isatidis 20 parts, Radix Scutellariae 15 parts, Radix Et Rhizoma Rhei 12 parts, Radix Adenophorae (Radix Glehniae) 12 parts, 12 parts, dry scallop, Herba Murdanniae Divergentis 12 parts, Radix Ophiopogonis 10 parts, Fructus Schisandrae Chinensis 10 parts, Radix Rehmanniae Preparata 8 parts and 10 parts, Radix Glycyrrhizae.
5. the purposes of the Chinese medicine composition as described in any one of claim 1-4 in preparation treatment wind-warm lung-heat medicine, is characterized in that: described Chinese medicine composition is made into capsule, tablet, powder or decoction.
CN201511003652.1A 2015-12-29 2015-12-29 Application of traditional Chinese medicine composition to preparation of medicine for treating wind-warm lung-heat disease Withdrawn CN105395836A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105770393A (en) * 2016-03-22 2016-07-20 广州暨南生物医药研究开发基地有限公司 Application of traditional Chinese medicine preparation containing Folium artemisiae argyi in preparing medicine for treating wind-warm lung-heat disease

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101062266A (en) * 2007-05-23 2007-10-31 陈江 Powder for treating cough and removing external heat in body

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101062266A (en) * 2007-05-23 2007-10-31 陈江 Powder for treating cough and removing external heat in body

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105770393A (en) * 2016-03-22 2016-07-20 广州暨南生物医药研究开发基地有限公司 Application of traditional Chinese medicine preparation containing Folium artemisiae argyi in preparing medicine for treating wind-warm lung-heat disease

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