CN105395614A - Lotus leaf alkaloid extracting method and application thereof - Google Patents
Lotus leaf alkaloid extracting method and application thereof Download PDFInfo
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- CN105395614A CN105395614A CN201510781970.4A CN201510781970A CN105395614A CN 105395614 A CN105395614 A CN 105395614A CN 201510781970 A CN201510781970 A CN 201510781970A CN 105395614 A CN105395614 A CN 105395614A
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- leaf alkaloid
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Abstract
The invention discloses a lotus leaf alkaloid extracting method and application thereof. Lotus leaves are smashed and extracted for 3 times through ethyl alcohol with different concentrations, extraction solutions are mixed, and acid is added into the mixture to convert lotus leaf alkaloid into lotus leaf alkaloid salt. The method is simple, operation is easy, requirements for equipment are low, the cost is saved, environmental pollution is avoided, and no harm is produced to the human body, and safety and health are achieved. The lotus leaf alkaloid insoluble in water is converted into lotus leaf alkaloid salt soluble in water, and the application range of the lotus leaf alkaloid is enlarged; the extracted lotus leaf alkaloid is applied in weight-losing health-care products, digestive absorption of the alkaloid is facilitated, and the health care functions of reducing blood fat, losing the weight, resisting bacteria and viruses, resisting oxidation and preventing cardiovascular and cerebrovascular diseases are better achieved in the body. The extraction rate and purity of the lotus leaf alkaloid is obviously improved, the extraction rate reaches 2.08%, the purity reaches 98.7%, and industrial production of the lotus leaf alkaloid is facilitated.
Description
Technical field
The present invention relates to natural drug and extract field, specifically, relate to a kind of lotus leaf alkaloid extracting method and application thereof.
Background technology
Folium Nelumbinis, nature and flavor are pained and put down, the heat clearing away of function expelling summer-heat, sending up the lucid YANG, hemostasis, diuretic.Folium Nelumbinis is the medicine-food two-purpose kind that health ministry is determined.History tree document and modern clinic be all on the books or checking to its dietary function.As " book on Chinese herbal medicine is new again " is called its " cooling and heatstroke-eliminating, slakes thirst and help produce saliva, and controls dysentery, separates burning hot ".Compendium of Material Medica is called its " hair growth promoting vigour, benefit helps taste, and arresting seminal emission is turbid, dissipating blood stasis blood, and detumescence pain, sends out variola ".Clinically be mainly used in summer-heat excessive thirst, diarrhea due to summer heat and dampness, aqueous vapor edema, it is hemorrhage to tell nosebleed, metrorrhagia hematochezia, puerperal blood faints etc.Folium Nelumbinis is put among the list of the 2nd batch " being food and medicine ".Show the chemical constitution study of Folium Nelumbinis, the chemical composition of Folium Nelumbinis mainly contains alkaloids, flavonoid, volatile oil and organic acid.Show the research of the modern pharmacology of Folium Nelumbinis, Folium Nelumbinis has the effect of antioxidation in vitro, antibacterial, antiviral and suppression lipase, and activity in vivo mainly contains blood fat reducing and effect of weight reducing.
Research finds that lotus leaf alkaloid constituents is one of Folium Nelumbinis major physiological active substance, the alkaloid kind had been found that in Folium Nelumbinis is more than 20 kinds, but alkaloid is insoluble in water in Folium Nelumbinis, its application is very restricted, and digest and assimilate slow, lotus leaf alkaloid is extracted in a salt form, there is good water solublity, can be used widely, and be conducive to human body and better absorb.
Summary of the invention
In order to make up the defect of prior art, the object of this invention is to provide a kind of lotus leaf alkaloid extracting method and application thereof.
A kind of lotus leaf alkaloid extracting method, concrete comprises the following steps:
(1) Folium Nelumbinis is cleaned, 40 ~ 50 DEG C of oven dry, pulverize, cross 60 ~ 80 mesh sieves, obtain Folium Nelumbinis powder;
(2) in Folium Nelumbinis powder, add the water of Folium Nelumbinis powder weight 2 ~ 4 times amount, stir, add the mixing enzyme preparation of Folium Nelumbinis powder weight 0.4 ~ 0.6%, 40 ~ 45 DEG C of water-baths, stir enzymolysis 3 ~ 4 hours, obtain enzymolysis Folium Nelumbinis slurry;
(3) in enzymolysis Folium Nelumbinis slurry, add enzymolysis Folium Nelumbinis slurry weight 3 ~ 4 times amount volumetric concentration is the alcoholic solution of 55%, 40 ~ 45 DEG C of supersound extraction 20 ~ 30 minutes, 6000 ~ 8000 revs/min, centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 ~ 3 times amount volumetric concentration is the alcoholic solution of 70%, 40 ~ 45 DEG C of supersound extraction 20 ~ 30 minutes, 7000 ~ 9000 revs/min, centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 ~ 3 times amount volumetric concentration is the alcoholic solution of 90%, supersound extraction 20 ~ 30 minutes, 8000 ~ 10000 revs/min, centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, merging filtrate, vacuum rotating is concentrated into 1/3 ~ 1/2 of original volume, ethanol reclaims, recycling, obtain lotus leaf alkaloid crude extract,
(4) in alkaloid crude extract, add alkaloid crude extract weight 2 ~ 3 times amount mass concentration is the aqueous solution of malic acid of 0.2 ~ 0.25%, and stir, vacuum rotating is concentrated into dry, obtains lotus leaf alkaloid extract, i.e. lotus leaf alkaloid salt.
Described step 2) mixing enzyme preparation be made up of the enzyme preparation of following weight ratio: cellulase: hemicellulase: lipase=7 ~ 8:4 ~ 5:1 ~ 2.
The application of a kind of lotus leaf alkaloid of the present invention, is using described lotus leaf alkaloid as blood fat reducing composition, in slimming health product, is made into oral liquid, every bottle of 5ml, and lotus leaf alkaloid content is 0.5mg/ml.
Advantage of the present invention is: a kind of lotus leaf alkaloid extracting method provided by the invention and application thereof, and method is simple, is easy to operation, low for equipment requirements, cost-saving, environmentally safe, to human body without harm, and safety and Health; The lotus leaf alkaloid being insoluble in water is converted into lotus leaf alkaloid salt soluble in water, expand the range of application of lotus leaf alkaloid, the lotus leaf alkaloid of extraction is applied in slimming health product, be conducive to alkaloidal digesting and assimilating, better play blood fat reducing in vivo, fat-reducing, antibacterial, antiviral, antioxidation, the health care of prevention cardiovascular and cerebrovascular disease; Extraction ratio and the purity of lotus leaf alkaloid are improved significantly, and extraction rate reached is to 2.08%, and purity reaches 98.7%, and the industrialization contributing to lotus leaf alkaloid is produced.
Detailed description of the invention
With specific embodiment, the present invention is described below, instead of restriction the present invention.
Embodiment 1
A kind of lotus leaf alkaloid extracting method, concrete comprises the following steps:
(1) Folium Nelumbinis is cleaned, 40 DEG C of oven dry, pulverize, cross 60 mesh sieves, obtain Folium Nelumbinis powder;
(2) in Folium Nelumbinis powder, add the water of Folium Nelumbinis powder weight 2 times amount, stir, add the mixing enzyme preparation of Folium Nelumbinis powder weight 0.4%, 40 DEG C of water-baths, stir enzymolysis 3 hours, obtain enzymolysis Folium Nelumbinis slurry;
(3) in enzymolysis Folium Nelumbinis slurry, add enzymolysis Folium Nelumbinis slurry weight 3 times amount volumetric concentration is the alcoholic solution of 55%, 40 DEG C of supersound extraction 20 minutes, 6000 revs/min, centrifugal 10 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 times amount volumetric concentration is the alcoholic solution of 70%, 40 DEG C of supersound extraction 20 minutes, 7000 revs/min, centrifugal 10 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 times amount volumetric concentration is the alcoholic solution of 90%, supersound extraction 20 minutes, 8000 revs/min, centrifugal 10 minutes, obtain filtering residue and filtrate, merging filtrate, vacuum rotating is concentrated into 1/3 of original volume, ethanol reclaims, recycling, obtain lotus leaf alkaloid crude extract,
(4) in alkaloid crude extract, add alkaloid crude extract weight 2 times amount mass concentration is the aqueous solution of malic acid of 0.2%, and stir, vacuum rotating is concentrated into dry, obtain lotus leaf alkaloid extract, i.e. lotus leaf alkaloid salt, extraction ratio is 1.97%, and purity is 98.4%.
Described step 2) mixing enzyme preparation be made up of the enzyme preparation of following weight ratio: cellulase: hemicellulase: lipase=7:4:1.
The application of a kind of lotus leaf alkaloid of the present invention, is using described lotus leaf alkaloid as blood fat reducing composition, in slimming health product, is made into oral liquid, every bottle of 5ml, and lotus leaf alkaloid content is 0.5mg/ml.
Embodiment 2
A kind of lotus leaf alkaloid extracting method, concrete comprises the following steps:
(1) Folium Nelumbinis is cleaned, 45 DEG C of oven dry, pulverize, cross 70 mesh sieves, obtain Folium Nelumbinis powder;
(2) in Folium Nelumbinis powder, add the water of Folium Nelumbinis powder weight 3 times amount, stir, add the mixing enzyme preparation of Folium Nelumbinis powder weight 0.5%, 43 DEG C of water-baths, stir enzymolysis 3.5 hours, obtain enzymolysis Folium Nelumbinis slurry;
(3) in enzymolysis Folium Nelumbinis slurry, add enzymolysis Folium Nelumbinis slurry weight 3.5 times amount volumetric concentration is the alcoholic solution of 55%, 43 DEG C of supersound extraction 25 minutes, 7000 revs/min, centrifugal 13 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2.5 times amount volumetric concentration is the alcoholic solution of 70%, 43 DEG C of supersound extraction 25 minutes, 8000 revs/min, centrifugal 13 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2.5 times amount volumetric concentration is the alcoholic solution of 90%, supersound extraction 25 minutes, 90000 revs/min, centrifugal 13 minutes, obtain filtering residue and filtrate, merging filtrate, vacuum rotating is concentrated into 1/2 of original volume, ethanol reclaims, recycling, obtain lotus leaf alkaloid crude extract,
(4) in alkaloid crude extract, add alkaloid crude extract weight 2.5 times amount mass concentration is the aqueous solution of malic acid of 0.23%, and stir, vacuum rotating is concentrated into dry, obtain lotus leaf alkaloid extract, i.e. lotus leaf alkaloid salt, extraction ratio is 2.04%, and purity is 98.6%.
Described step 2) mixing enzyme preparation be made up of the enzyme preparation of following weight ratio: cellulase: hemicellulase: lipase=7:5:2.
The application of a kind of lotus leaf alkaloid of the present invention, is using described lotus leaf alkaloid as blood fat reducing composition, in slimming health product, is made into oral liquid, every bottle of 5ml, and lotus leaf alkaloid content is 0.5mg/ml.
Embodiment 3
A kind of lotus leaf alkaloid extracting method, concrete comprises the following steps:
(1) Folium Nelumbinis is cleaned, 50 DEG C of oven dry, pulverize, cross 80 mesh sieves, obtain Folium Nelumbinis powder;
(2) in Folium Nelumbinis powder, add the water of Folium Nelumbinis powder weight 4 times amount, stir, add the mixing enzyme preparation of Folium Nelumbinis powder weight 0.6%, 45 DEG C of water-baths, stir enzymolysis 4 hours, obtain enzymolysis Folium Nelumbinis slurry;
(3) in enzymolysis Folium Nelumbinis slurry, add enzymolysis Folium Nelumbinis slurry weight 4 times amount volumetric concentration is the alcoholic solution of 55%, 45 DEG C of supersound extraction 30 minutes, 8000 revs/min, centrifugal 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 3 times amount volumetric concentration is the alcoholic solution of 70%, 45 DEG C of supersound extraction 30 minutes, 9000 revs/min, centrifugal 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 3 times amount volumetric concentration is the alcoholic solution of 90%, supersound extraction 30 minutes, 10000 revs/min, centrifugal 15 minutes, obtain filtering residue and filtrate, merging filtrate, vacuum rotating is concentrated into 1/2 of original volume, ethanol reclaims, recycling, obtain lotus leaf alkaloid crude extract,
(4) in alkaloid crude extract, add alkaloid crude extract weight 3 times amount mass concentration is the aqueous solution of malic acid of 0.25%, and stir, vacuum rotating is concentrated into dry, obtain lotus leaf alkaloid extract, i.e. lotus leaf alkaloid salt, extraction ratio is 2.08%, and purity is 98.7%.
Described step 2) mixing enzyme preparation be made up of the enzyme preparation of following weight ratio: cellulase: hemicellulase: lipase=8:5:2.
The application of a kind of lotus leaf alkaloid of the present invention, is using described lotus leaf alkaloid as blood fat reducing composition, in slimming health product, is made into oral liquid, every bottle of 5ml, and lotus leaf alkaloid content is 0.5mg/ml.
Comparative example
Commercially available common slimming health product.
The Function detection of lotus leaf alkaloid oral liquid:
Usage and dosage:
Every day 2 times, each 1 bottle of oral liquid 10 days is a course for the treatment of.
Curative effect determinate standard:
More than weight loss 1Kg, is effectively;
Body weight is constant, even higher, be then invalid;
Test case:
Stochastic choice patient 400 example, all meets obesity standard, continues fat more than 1 year.At random above-mentioned case is divided into treatment group and test group, each 100 people.All patients do not take other fat-reducing medicine, and do not carry out Weight reducing behavior deliberately, the tested time is a course for the treatment of.The fat-reducing effect of lotus leaf alkaloid is in table 1.
Table 1: the fat-reducing effect of lotus leaf alkaloid
| Project | Patient's number | Effectively | Invalid |
| Embodiment 1 | 100 | 94（94%） | 6（6%） |
| Embodiment 2 | 100 | 96（96%） | 4（4%） |
| Embodiment 3 | 100 | 97（97%） | 3（3%） |
| Comparative example | 100 | 67（67%） | 33（33%） |
The result of table 1 shows, the fat-reducing effective percentage of embodiment lotus leaf alkaloid, apparently higher than the slimming health product of comparative example, has obvious fat-reducing effect.
Model case:
(1) patient Lee, female, 38 years old, height 162cm, body weight 75Kg, continued fat 3 years.Take slimming health product provided by the invention, weight loss 5.2Kg after 10 days.
(2) Guo patient, man, 48 years old, height 172cm, body weight 82Kg, continued fat 6 years.Take slimming health product provided by the invention, weight loss 6.5Kg after 10 days.
(3) patient Zhao, female, 53 years old, height 167cm, body weight 79Kg, continued fat 8 years.Take slimming health product provided by the invention, weight loss 7.3Kg after 10 days.
Claims (3)
1. a lotus leaf alkaloid extracting method, is characterized in that, comprises the following steps:
(1) Folium Nelumbinis is cleaned, 40 ~ 50 DEG C of oven dry, pulverize, cross 60 ~ 80 mesh sieves, obtain Folium Nelumbinis powder;
(2) in Folium Nelumbinis powder, add the water of Folium Nelumbinis powder weight 2 ~ 4 times amount, stir, add the mixing enzyme preparation of Folium Nelumbinis powder weight 0.4 ~ 0.6%, 40 ~ 45 DEG C of water-baths, stir enzymolysis 3 ~ 4 hours, obtain enzymolysis Folium Nelumbinis slurry;
(3) in enzymolysis Folium Nelumbinis slurry, add enzymolysis Folium Nelumbinis slurry weight 3 ~ 4 times amount volumetric concentration is the alcoholic solution of 55%, 40 ~ 45 DEG C of supersound extraction 20 ~ 30 minutes, under 6000 ~ 8000 revs/min centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 ~ 3 times amount volumetric concentration is the alcoholic solution of 70%, 40 ~ 45 DEG C of supersound extraction 20 ~ 30 minutes, under 7000 ~ 9000 revs/min centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, in filtering residue, add filtering residue weight 2 ~ 3 times amount volumetric concentration is the alcoholic solution of 90%, supersound extraction 20 ~ 30 minutes, under 8000 ~ 10000 revs/min centrifugal 10 ~ 15 minutes, obtain filtering residue and filtrate, merging filtrate, vacuum rotating is concentrated into 1/3 ~ 1/2 of original volume, ethanol reclaims, recycling, obtain lotus leaf alkaloid crude extract,
(4) in alkaloid crude extract, add alkaloid crude extract weight 2 ~ 3 times amount mass concentration is the aqueous solution of malic acid of 0.2 ~ 0.25%, and stir, vacuum rotating is concentrated into dry, obtains lotus leaf alkaloid extract, i.e. lotus leaf alkaloid salt.
2. a kind of lotus leaf alkaloid extracting method according to claim 1, is characterized in that, described step 2) mixing enzyme preparation be made up of the enzyme preparation of following weight ratio: cellulase: hemicellulase: lipase=7 ~ 8:4 ~ 5:1 ~ 2.
3. an application for lotus leaf alkaloid according to claim 1, is characterized in that, is using described lotus leaf alkaloid as blood fat reducing composition, in slimming health product, is made into oral liquid, every bottle of 5ml, and lotus leaf alkaloid content is 0.5mg/ml.
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| CN201510781970.4A CN105395614A (en) | 2015-11-16 | 2015-11-16 | Lotus leaf alkaloid extracting method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108094696A (en) * | 2017-12-27 | 2018-06-01 | 福建光阳蛋业股份有限公司 | A kind of laying hen lotus leaf feed and preparation method thereof |
| CN108271976A (en) * | 2018-01-22 | 2018-07-13 | 郑州木村农业科技有限公司 | One kind subtracting body fat and promotes immunity Lotus Leafextract health beverages and its manufacturing method |
| CN113773131A (en) * | 2021-07-05 | 2021-12-10 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
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| CN101007064A (en) * | 2006-09-09 | 2007-08-01 | 福州大学 | Isolation and purification method of lotus leaf extract by macroporous resin and its application thereof |
| CN101007063A (en) * | 2006-09-09 | 2007-08-01 | 福州大学 | Preparation method of lotus leaf extract and use thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094696A (en) * | 2017-12-27 | 2018-06-01 | 福建光阳蛋业股份有限公司 | A kind of laying hen lotus leaf feed and preparation method thereof |
| CN108271976A (en) * | 2018-01-22 | 2018-07-13 | 郑州木村农业科技有限公司 | One kind subtracting body fat and promotes immunity Lotus Leafextract health beverages and its manufacturing method |
| CN113773131A (en) * | 2021-07-05 | 2021-12-10 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
| CN113773131B (en) * | 2021-07-05 | 2022-08-05 | 河南正达作物保护有限公司 | Preparation and application method of fertilizer synergist |
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Application publication date: 20160316 |