CN105394011A - Rice moth egg double-sided embryo killing method - Google Patents
Rice moth egg double-sided embryo killing method Download PDFInfo
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- CN105394011A CN105394011A CN201510715037.7A CN201510715037A CN105394011A CN 105394011 A CN105394011 A CN 105394011A CN 201510715037 A CN201510715037 A CN 201510715037A CN 105394011 A CN105394011 A CN 105394011A
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- rice moth
- moth egg
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/22—Killing insects by electric means
- A01M1/226—Killing insects by electric means by using waves, fields or rays, e.g. sound waves, microwaves, electric waves, magnetic fields, light rays
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Cereal-Derived Products (AREA)
Abstract
The invention discloses a rice moth egg double-sided embryo killing method, comprising a rice moth egg double-sided embryo killing cabinet. The method is characterized by also comprising steps of freezing egg carrying boards, forming a basal plate water film, making egg carrying boards, and irradiating to kill embryos. The method makes density of a rice moth egg formed on the egg carrying board reach 300 ml/m<2>, and rice moth egg handling capacity is 3.75 times of density of an artificial spawning method. Since double surfaces of the basal plate can be used, coated rice moth eggs are 7 times of an original method on a same-area basal plate, and coating operation time is reduced by 70%, so productivity is greatly improved. The method is simple and rapid in rice moth egg coating operation, and stable in effect. Rice moth egg coating density is large, close to theoretical density. An ultraviolet lamp irradiation device is simple in structure, and the method is suitable for different production scales. The method advantaged by high embryo killing efficiency, and is suitable for present requirement of trichogramma expanding propagation production.
Description
Technical field
The present invention relates to a kind of method of breeding production oophagous trichogrammae, rice moth egg kills embryo method.
Background technology
South China area extensively grows cane as the raw material of industry sugaring.In cane planting process, sugarcane yield and quality have the greatest impact because sugarcane moth borer encroaches on, and serious insect pest can make the sugarcane yield underproduction 10 ~ 15%, and the sugary part of sugarcane declines 3 ~ 5%.For obtaining stable yields good harvest, current large-scale production oophagous trichogrammae control sugarcane moth borer is progressively launched in south China area.Application oophagous trichogrammae control sugarcane moth borer, being because biological insect protected has better pest control effect than using chemical insecticide, also having no adverse effects to natural environment.
Oophagous trichogrammae is the parasitic wasp that a class bodily form is small, and usual body is long less than 1 millimeter, gains the name because having red simple eye and compound eye.Its ovum produces host by queen bee, as snout moth's larva in the ovum that produces, and develop into bee pupa wherein, make the ovum of snout moth's larva can not normal development and lose breeding effect, thus reach and indirectly kill snout moth's larva effect.Sugarcane production early stage season, natural world oophagous trichogrammae quantity is few, and deficiency effectively kills a large amount of sugarcane moth borer.Therefore, a large amount of oophagous trichogrammae bee pupas need be brought out by artificial breeding, and bee pupa is seated in cane-growing region.Bee pupa then develops into oophagous trichogrammae naturally, reaches and prevents and treats sugarcane moth borer object by honeybee.
For large-scale breeding produces oophagous trichogrammae, need first mass rearing rice moth in its production process, and obtain rice moth egg.Rice moth egg is a kind of host egg for Trichogramma, is usually used in factorial praluction oophagous trichogrammae.Shown in oophagous trichogrammae Reproduction technological process Fig. 1, as can be known from Fig. 1, it is the important bottleneck point that impact improves oophagous trichogrammae production efficiency that operation 4 rice moth egg kills embryo, kills rice moth egg embryo, in order to avoid go out worm.Rice moth egg kills embryo method and usually adopts ultraviolet light one side irradiation, and this kills embryo operating procedure is be laid on a flat face, as shown in Figure 2 with Fig. 3 by needing the rice moth egg carrying out killing embryo process.Because ultraviolet penetration capacity is weak, between the rice moth egg on flat spreading part, can not overlapping phenomenon be there is, for more than 2 overlapping rice moth eggs because of ultraviolet light not transmissive, non-illumination to moth ovum just lose " killing embryo " effect.Within about 20 ~ 30 minutes, namely complete " killing embryo " process with ultraviolet lamp certain distance internal radiation rice moth egg above rice moth egg, the rice moth egg being only subject to ultraviolet light direct irradiation just produces " killing embryo " effect.Thus kill embryo technological requirement rice moth egg can only single tiling to be subject to the irradiation of ultraviolet light, thus, kill the maximum difficult point of embryo technique and be with manually rice moth egg being laid in the operation of " plane ".
" rice moth egg tiling " method normally at present: by manually being sowed on multiple " flat stainless steel pallet " by rice moth egg, the non-overlapping effect thus acquisition moth ovum tiles.Its shortcoming is that rice moth egg tiling density is loose uneven, or also has overlapping, be generally 80 milliliters of rice moth eggs/square metre, as shown in Figure 1.As according to above-mentioned " rice moth egg tiling " method, everyone can only kill embryo process 20000 milliliters of rice moth eggs usually every day.Not only labour cost is high, wholely kills embryo inefficiency and can not meet production requirement.
Summary of the invention
The object of the invention is to provide one and can completes fast, in a large number and kill embryo to rice moth egg, and can reach that the stable rice moth egg killing embryo effect is two-sided kills embryo method.
For reaching above-mentioned purpose, technical scheme of the present invention is:
Kill embryo technique to be made up of UV-irradiation case and rice moth egg coating process, process is as follows:
UV-irradiation case making → substrate manufacture → substrate is freezing → and substrate moisture film generation → base plate coating rice moth egg (carrying ovum plate) → ultraviolet light double-sided illumination → inactivation rice moth egg gathers.
Concrete as being:
A kind of rice moth egg is two-sided kills embryo case, and it includes the airtight casing of door, characterized by further comprising:
Primarily of some Ultraviolet radiation layers that substrate and quartz burner are formed, wherein, every layer of Ultraviolet radiation layer comprises some block gaps level and is all placed in substrate in casing, and quartz burner interval is also horizontally placed on above and below substrate;
The bracket be made up of case inner column and needle holder, wherein, needle holder is that L shape, one end and column or chamber interior wall are fixedly connected, and below one jiao that other end needle point holds substrate upward, the needle holder at every 4 intervals supports one piece of substrate.
Described substrate, for comprising two surfacing, smooth and clean, has the glass plate of good hydrophilic characteristic, plank or rigid plastic sheet.
A kind of rice moth egg is two-sided kills embryo method, and it comprises, and rice moth egg is two-sided kills embryo case, characterized by further comprising following steps:
(1) carry ovum plate freezing, ovum plate will be carried and put into temperature for-15 DEG C of refrigerators, and place on 5 ~ 15 minutes;
(2) substrate moisture film generates, ovum plate will be carried take out from refrigerator, and be exposed to 25 DEG C, under the room temperature of humidity >=80%RH, make substrate two sides form the thin and even frost face of one deck immediately owing to carrying the heat absorption of ovum plate, frost face heat absorption subsequently and become the thin and uniform moisture film of one deck fast;
(3) carry ovum plate to make, during the two-sided formation moisture film of substrate, manually ready rice moth egg is sowed rapidly on the two-sided moisture film of substrate, make on the two sides of rice moth egg covered substrate, meanwhile, manually continuous above collection ovum dish upset be loaded with the substrate of rice moth egg, make the uniform viscous of rice moth egg on the two sides of substrate, form a year ovum plate;
(4) embryo is killed in irradiation, being neatly placed horizontally on the two-sided needle holder killed in embryo case of rice moth egg, closing upper chamber door by carrying an ovum plate; Carry ovum plate 20 ~ 30 minute after to carrying ovum plate from upper and lower two-way irradiation through ultraviolet light, rice moth egg kills embryo and completes;
(5) inactivation rice moth egg is gathered, and carries the rice moth egg of ovum plate after irradiating and killing embryo, embryo lose grow active, workman with hairbrush by inactivation rice moth egg from support plate brush, collect and refrigerate, preparation enters next production process.
Described refrigerator comprises refrigerator, freezer and refrigerator-freezer.
Adopt the rice moth egg layer density that said method gained carries the formation of ovum plate to reach 300 milliliters/square metre, rice moth egg treating capacity is 3.75 times that adopt artificial broadcast's method density.Utilized because substrate is two-sided, same substrate area coating rice moth egg is former method 7 times, and painting work time decreased 70%, improve productivity ratio greatly.And have the following advantages:
1, rice moth egg painting work method simply and fast, effect stability;
2, rice moth egg coating density is large, close to solid density;
3, ultra violet lamp apparatus structure is simple, adapts to different production scales;
4, kill embryo efficiency high, adapt to current oophagous trichogrammae and expand numerous production requirement.
Accompanying drawing explanation
Fig. 1 is existing oophagous trichogrammae Reproduction process chart;
Fig. 2 is that existing pallet tiling rice moth egg one side uviol lamp is according to killing embryo schematic diagram;
Fig. 3 is the structural representation that Fig. 2 analyses and observe along A-A line;
To be that rice moth egg is two-sided kill embryo box structure schematic diagram to Fig. 4;
Fig. 5 is the structural representation that Fig. 4 analyses and observe along B-B line;
Fig. 6 is the two-sided principle schematic of killing embryo of rice moth egg of the present invention;
In the drawings, casing 1, quartz burner 2, column 3, needle holder 4, substrate 5, chamber door 6.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
Shown in Fig. 1, be existing oophagous trichogrammae Reproduction process chart, existing oophagous trichogrammae Reproduction needs to kill embryo through comprising operation 4 rice moth egg, it is the important bottleneck point that impact improves oophagous trichogrammae production efficiency, kill rice moth egg embryo, in order to avoid go out worm, oophagous trichogrammae just can be made to expand numerous at rice moth egg parasitism.
Shown in Fig. 2, that existing pallet tiling rice moth egg one side uviol lamp is according to killing embryo schematic diagram, Fig. 3 is the structural representation that Fig. 2 analyses and observe along A-A line, two figure combine known, it is to tile rice moth egg with stainless steel pallet that existing rice moth egg kills embryo method, kill embryo by the ultra violet lamp be located at above rice moth egg, rice moth egg is wherein sprawled density is loose uneven, or also has overlapping.
Shown in Fig. 4, being that rice moth egg is two-sided kills embryo box structure schematic diagram, and the main casing 1 of this case, ultraviolet lamp tube 2 and substrate 5 bracket three part form.It is characterized in that casing 1 is made up of plank, iron plate, plastic plate or other plate, high by 200 × wide by 130 × dark 45cm also has chamber door 6 to be opened/closed, or replace with gobo.The bracket of quartz burner 2 and substrate 5 and supporting substrates 5 is provided with in casing 1.Wherein, bracket is made up of column 3 and needle holder 4, and composition graphs 4 comes together to see along structural representation Fig. 5 that B-B line is analysed and observe, totally four, column 3, and perpendicular separation is fixed in casing 1 respectively; Every bar column 3 is fixed with ten needle points needle holder 4 upward, all the other 20 needle holder 4 correspondences are fixed in case lateral plates.Every 4 needle holders can support a substrate 5.Make ultraviolet light can from upper, up and down two the plate faces of lower direction to substrate be carried out shadow-free illumination and are penetrated.Needle holder 4 is L shape, and its one end and column 3 or chamber interior wall are fixedly connected, and other end needle point is held below substrate 5 one jiaos upward, and the needle holder 4 at every four intervals supports one piece of substrate 5.Open chamber door 6, can conveniently put/be taken into substrate 5; Close chamber door 6, can prevent the outer light of bavin from leaking.In casing 1, spaced horizontal places 12 quartz burners 2, is divided into 6 layers.Every layer of 2 quartz burner 2, from being about 25cm between level.Every layer of quartz burner about 2 is at a distance of=40cm.Whole fluorescent tube is carried out time switch by electronic switch and is controlled, and has quartz burner fault alarm function.
Substrate 6 is made up of one block of thin glass or rigid plastic sheet, and " substrate " feature is: shape is rectangle, two surfacing, smooth, clean, has good water-wet behavior; Size is 300 × 400mm about, thick 2mm, and edge polishes cunning, and size can be how many and change according to coating rice moth egg amount.
15 pieces of substrates, 5 pairs of rice moth eggs can be loaded in a casing 1 carry out illumination and kill embryo process simultaneously.According to this structure, according to technological requirement, different size casing can be made, adapt to different treating capacity requirement of killing embryo.Above-mentioned casing 1, quartz burner 2, column 3, needle holder 4, substrate 5 and chamber door 6 form that rice moth egg is two-sided kills embryo case.
Utilize that rice moth egg is two-sided to kill the method that embryo case carries out killing embryo and be:
1, process by producing the requirement that rice moth egg kills embryo aequum, according to the two-sided embryo case that kills of rice moth egg that the quantity of structure fabrication shown in Fig. 4 and Fig. 5 does not wait, stand-by;
2, process the requirement that rice moth egg kills embryo aequum, the substrate 5 do not waited by the quantity of structure fabrication shown in Fig. 4 and Fig. 5 by producing, and clean; Then whole substrate 5 is put into temperature for-15 DEG C of freezers, place 5 ~ 15 minutes, stand-by;
3, coating rice moth egg operation outfit and material is manually carried out: as rice moth egg, collect ovum pallet, building paper, hairbrush etc.Then, manually substrate 5 is taken out one piece from freezer, and be exposed to 25 DEG C, under the room temperature of humidity >=80%RH, make substrate two sides form the thin and even frost face of one deck immediately owing to carrying the heat absorption of ovum plate, wait a moment, frost face heat absorption and become the thin and uniform moisture film of one deck fast, by ready rice moth egg rapidly by sow substrate 5 two-sided on, and by areas whole for rice moth egg overlay face, meanwhile, manually constantly above collection ovum dish, upset is loaded with the substrate 5 of rice moth egg; After eight seconds, rice moth egg just inhale by the moisture film on substrate 5 and be bonded on plate face, when many and tiny rice moth egg contacts with substrate 5, form goo after rice moth egg surface protein contacts with water rice moth egg to be tightly bonded on the two sides of substrate 5, whole substrate 5 two-sided formation respectively one deck is smooth and the rice moth egg layer of densification forms a year ovum plate, complete rice moth egg fast to tile in the coating that substrate 5 is two-sided, complete the rice moth egg painting work process of a substrate 5.So analogize, operate base plate coating one by one, until all required rice moth egg is applied complete till.The rice moth egg do not contacted with plate face also nature drops on the collection ovum dish of below from substrate 5, the coating again for same way after being collected.The substrate 5 being coated with rice moth egg is called a year ovum plate;
4, being manually neatly placed horizontally on the needle holder of UV-irradiation case by carrying ovum plate block-by-block, closing upper chamber door 6.Switch on power, ultraviolet lamp tube 2 is worked, light carries ovum plate after 20 ~ 30 minutes to carrying ovum plate from upper and lower two-way irradiation, and rice moth egg kills embryo process and completes;
The coating of above-mentioned rice moth egg with kill embryo operating efficiency and improve more than 5 than existing technique, and reduce quartz burner number 40%.Embryo production efficiency is killed in very big raising, eliminates oophagous trichogrammae scale production process bottleneck;
5, inactivation rice moth egg is gathered, and closes ultraviolet lamp tube power supply, is taken out by the ovum plate block-by-block that carries in UV-irradiation case by workman, then with hairbrush by inactivation rice moth egg from support plate block-by-block brush, collect and refrigerate, the whole embryo operating procedure that kills completes.
Claims (5)
1. rice moth egg is two-sided kills an embryo case, and it includes the airtight casing of door, characterized by further comprising:
Primarily of some Ultraviolet radiation layers that substrate and quartz burner are formed, wherein, every layer of Ultraviolet radiation layer comprises some block gaps level and is all placed in substrate in casing, and quartz burner interval is also horizontally placed on above and below substrate;
The bracket be made up of case inner column and needle holder, wherein, needle holder is that L shape, one end and column or chamber interior wall are fixedly connected, and below one jiao that other end needle point holds substrate upward, the needle holder at every 4 intervals supports one piece of substrate.
2. according to claim 1ly kill embryo case, it is characterized in that:
Described substrate, for comprising two surfacing, smooth and clean, has the glass plate of good hydrophilic characteristic, plank or rigid plastic sheet.
3. according to claim 1ly kill embryo case, it is characterized in that:
In casing, spaced horizontal places 12 quartz burners, is divided into 6 layers; Every layer of 2 quartz burner, from being about 25cm between level; Every layer of quartz burner apart=40cm up and down; Whole fluorescent tube is carried out time switch by electronic switch and is controlled, and has quartz burner fault alarm function.
4. rice moth egg is two-sided kills an embryo method, and it comprises, and rice moth egg is two-sided kills embryo case, characterized by further comprising following steps:
(1) carry ovum plate freezing, ovum plate will be carried and put into temperature for-15 DEG C of refrigerators, and place 5 ~ 15 minutes;
(2) substrate moisture film generates, ovum plate will be carried take out from refrigerator, and be exposed to 25 DEG C, under the room temperature of humidity >=80%RH, make substrate two sides form the thin and even frost face of one deck immediately owing to carrying the heat absorption of ovum plate, frost face heat absorption subsequently and become the thin and uniform moisture film of one deck fast;
(3) carry ovum plate to make, during the two-sided formation moisture film of substrate, manually ready rice moth egg is sowed rapidly on the two-sided moisture film of substrate, make on the two sides of rice moth egg covered substrate, meanwhile, manually continuous above collection ovum dish upset be loaded with the substrate of rice moth egg, make the uniform viscous of rice moth egg on substrate, form a year ovum plate;
(4) embryo is killed in irradiation, being neatly placed horizontally on the two-sided needle holder killed in embryo case of rice moth egg, closing upper chamber door by carrying an ovum plate; Carry ovum plate 20 ~ 30 minute after to carrying ovum plate from upper and lower two-way irradiation through ultraviolet light, rice moth egg kills embryo and completes;
(5) inactivation rice moth egg is gathered, and carries the rice moth egg of ovum plate after irradiating and killing embryo, embryo lose grow active, workman with hairbrush by inactivation rice moth egg from support plate brush, collect and refrigerate, preparation enters next production process.
5. method according to claim 4, is characterized in that:
Described refrigerator comprises refrigerator, freezer and refrigerator-freezer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510715037.7A CN105394011A (en) | 2015-10-29 | 2015-10-29 | Rice moth egg double-sided embryo killing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510715037.7A CN105394011A (en) | 2015-10-29 | 2015-10-29 | Rice moth egg double-sided embryo killing method |
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| CN105394011A true CN105394011A (en) | 2016-03-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122629A (en) * | 2018-08-15 | 2019-01-04 | 溧阳益植生物技术有限公司 | A kind of insect ovum efficiently kills embryo case and its kills embryo method |
| CN110720444A (en) * | 2019-11-19 | 2020-01-24 | 吉林农业大学 | Method and device for dedusting and embryo killing of rice moth eggs through low-frequency vibration transmission |
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| CN204630237U (en) * | 2015-03-27 | 2015-09-09 | 四川农业大学 | Multiaspect ultraviolet irradiation sterilizing unit |
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2015
- 2015-10-29 CN CN201510715037.7A patent/CN105394011A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN204630237U (en) * | 2015-03-27 | 2015-09-09 | 四川农业大学 | Multiaspect ultraviolet irradiation sterilizing unit |
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| 叶正楚等: "用紫外线处理的米蛾卵饲养草蛉", 《生物防治通报》 * |
| 杨丽文等: "米蛾卵饲养东亚小花蝽的关键点研究", 《环境昆虫学报》 * |
| 袁曦等: "紫外线处理米蛾卵对赤眼蜂繁殖的影响", 《广东农业科学》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109122629A (en) * | 2018-08-15 | 2019-01-04 | 溧阳益植生物技术有限公司 | A kind of insect ovum efficiently kills embryo case and its kills embryo method |
| CN110720444A (en) * | 2019-11-19 | 2020-01-24 | 吉林农业大学 | Method and device for dedusting and embryo killing of rice moth eggs through low-frequency vibration transmission |
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